Plant Science最新文献_第3页

Field-deployable CRISPR-cas variants for rapid on-site detection of plant pathogens 用于快速现场检测植物病原体的可现场部署的CRISPR-Cas变体。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-02-05 DOI: 10.1016/j.plantsci.2026.113028

Himanshu Saini , Jyoti Yadav , Sharad Pandey , Anand Kumar , Deepak Nanda , Sharad Sachan , Jeevan Jyoti Kaushik

Rapid, field-deployable diagnostics are essential for effective plant disease management. Although CRISPR-Cas systems offer high sensitivity and programmability, their use in on-site plant pathogen detection has been hindered by the lack of standardized, practical workflows. Here we present implementable CRISPR-Cas diagnostic protocols using Cas12a, Cas13a, and miniature Cas variants for rapid detection of major plant pathogens. Three field-ready assays are described: (i) an RPA-Cas12a lateral-flow test for DNA pathogens, (ii) a Cas13a RT-RPA assay for RNA viruses, and (iii) an amplification-free Cas12a electrochemical biosensor suited for portable laboratories. Each protocol includes sample preparation steps, reagent formulations, incubation conditions, and troubleshooting guidance. Across platforms, detection limits of 1–100 copies µL⁻¹ were achieved within 20–45 min, demonstrating analytical sensitivity comparable to conventional PCR-based diagnostics while offering substantially reduced assay time and improved field deployability. We also address practical constraints including sample inhibitors, reagent stability, and biosafety and propose solutions for field implementation. These standardized workflows translate recent advances in CRISPR diagnostics into reproducible, field-deployable tools for plant health surveillance and rapid disease detection.

快速、可现场部署的诊断对于有效的植物病害管理至关重要。尽管CRISPR-Cas系统具有高灵敏度和可编程性，但由于缺乏标准化、实用的工作流程，它们在现场植物病原体检测中的应用一直受到阻碍。在这里，我们提出了可实现的CRISPR-Cas诊断方案，使用Cas12a、Cas13a和微型Cas变体来快速检测主要植物病原体。本文描述了三种现成的现场检测方法：(i) DNA病原体的RPA-Cas12a横向流动检测，（ii） RNA病毒的Cas13a RT-RPA检测，以及（iii）适用于便携式实验室的无扩增Cas12a电化学生物传感器。每个方案包括样品制备步骤，试剂配方，培养条件和故障排除指导。在各个平台上，在20-45分钟内实现了1-100 μ L的检测限，证明了与传统pcr诊断相当的分析灵敏度，同时大大缩短了分析时间，提高了现场部署能力。我们还解决了包括样品抑制剂，试剂稳定性和生物安全性在内的实际限制，并提出了现场实施的解决方案。这些标准化工作流程将CRISPR诊断的最新进展转化为可重复、可现场部署的工具，用于植物健康监测和快速疾病检测。

{"title":"Field-deployable CRISPR-cas variants for rapid on-site detection of plant pathogens","authors":"Himanshu Saini , Jyoti Yadav , Sharad Pandey , Anand Kumar , Deepak Nanda , Sharad Sachan , Jeevan Jyoti Kaushik","doi":"10.1016/j.plantsci.2026.113028","DOIUrl":"10.1016/j.plantsci.2026.113028","url":null,"abstract":"<div><div>Rapid, field-deployable diagnostics are essential for effective plant disease management. Although CRISPR-Cas systems offer high sensitivity and programmability, their use in on-site plant pathogen detection has been hindered by the lack of standardized, practical workflows. Here we present implementable CRISPR-Cas diagnostic protocols using Cas12a, Cas13a, and miniature Cas variants for rapid detection of major plant pathogens. Three field-ready assays are described: (i) an RPA-Cas12a lateral-flow test for DNA pathogens, (ii) a Cas13a RT-RPA assay for RNA viruses, and (iii) an amplification-free Cas12a electrochemical biosensor suited for portable laboratories. Each protocol includes sample preparation steps, reagent formulations, incubation conditions, and troubleshooting guidance. Across platforms, detection limits of 1–100 copies µL⁻¹ were achieved within 20–45 min, demonstrating analytical sensitivity comparable to conventional PCR-based diagnostics while offering substantially reduced assay time and improved field deployability. We also address practical constraints including sample inhibitors, reagent stability, and biosafety and propose solutions for field implementation. These standardized workflows translate recent advances in CRISPR diagnostics into reproducible, field-deployable tools for plant health surveillance and rapid disease detection.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113028"},"PeriodicalIF":4.1,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146137837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

C2H2-type zinc finger proteins: Contributing regulators of plant tolerance to abiotic stress c2h2型锌指蛋白：植物抗非生物胁迫的重要调节因子。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-02-04 DOI: 10.1016/j.plantsci.2026.113023

Ying Qu, Yunxuan Feng, Pengguo Xia

C2H2-type zinc finger proteins, as a class of key transcription factors in plants, play a important regulatory role in plant response to a wide range of abiotic stresses, including salt stress, drought, temperature stress, osmotic stress, high light stress and oxidative stress. This paper presents a systematic review of the structural characterization and classification system of C2H2-type zinc finger proteins and their molecular mechanisms involved in abiotic stress response. Research indicates that C2H2-type zinc finger proteins enhance plant stress resistance indirectly by regulating ion homeostasis, promoting the synthesis of osmoregulatory substances, activating reactive oxygen species scavenging systems, and participating in both ABA-dependent and ABA-independent signaling pathways. In addition, these proteins can indirectly enhance plant adaptation to stressful environments by regulating physiological processes such as stomatal movement and photosynthetic efficiency. This study provides new theoretical basis and strategic ideas for addressing the challenges of global climate change and ensuring food security.

c2h2型锌指蛋白是植物体内的一类关键转录因子，在植物对盐胁迫、干旱胁迫、温度胁迫、渗透胁迫、强光胁迫和氧化胁迫等多种非生物胁迫的响应中发挥重要的调控作用。本文对c2h2型锌指蛋白的结构特征、分类体系及其参与非生物胁迫反应的分子机制进行了系统综述。研究表明，c2h2型锌指蛋白通过调节离子稳态、促进渗透调节物质的合成、激活活性氧清除系统以及参与aba依赖和aba不依赖的信号通路间接增强植物的抗逆性。此外，这些蛋白质可以通过调节气孔运动和光合效率等生理过程间接增强植物对逆境环境的适应能力。该研究为应对全球气候变化挑战、保障粮食安全提供了新的理论依据和战略思路。

{"title":"C2H2-type zinc finger proteins: Contributing regulators of plant tolerance to abiotic stress","authors":"Ying Qu, Yunxuan Feng, Pengguo Xia","doi":"10.1016/j.plantsci.2026.113023","DOIUrl":"10.1016/j.plantsci.2026.113023","url":null,"abstract":"<div><div><em>C2H2</em>-type zinc finger proteins, as a class of key transcription factors in plants, play a important regulatory role in plant response to a wide range of abiotic stresses, including salt stress, drought, temperature stress, osmotic stress, high light stress and oxidative stress. This paper presents a systematic review of the structural characterization and classification system of <em>C2H2-</em>type zinc finger proteins and their molecular mechanisms involved in abiotic stress response. Research indicates that <em>C2H2-</em>type zinc finger proteins enhance plant stress resistance indirectly by regulating ion homeostasis, promoting the synthesis of osmoregulatory substances, activating reactive oxygen species scavenging systems, and participating in both ABA-dependent and ABA-independent signaling pathways. In addition, these proteins can indirectly enhance plant adaptation to stressful environments by regulating physiological processes such as stomatal movement and photosynthetic efficiency. This study provides new theoretical basis and strategic ideas for addressing the challenges of global climate change and ensuring food security.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113023"},"PeriodicalIF":4.1,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146132913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A positive feedback loop between the lncRNA TaHTMAR and TaHGSNAT is essential for thermo-sensitive male fertility in wheat lncRNA TaHTMAR和tahtsnat之间的正反馈回路是小麦温敏雄性育性的必要条件。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-02-04 DOI: 10.1016/j.plantsci.2026.113022

Xiaoran Gao, Qiang Geng, Xin Che, Fuqiang Niu, Jinke Liu, Lingling Hu, Xiaoxia Wang, Xiyue Song, Lingli Zhang

Wheat thermo-sensitive male sterility with Aegilops kotschyi cytoplasm (K-TCMS) is a promising system for hybrid breeding, yet the molecular mechanisms governing fertility conversion remain elusive. In the K-TCMS line KTM3315A, we identified a gene pair: TaHGSNAT (encoding a heparin-α-glucosaminide N-acetyltransferase) and its antisense long non-coding RNA, TaHTMAR. These two transcripts synchronized co-expression patterns during pollen development. Distinct subcellular localizations (membrane for TaHGSNAT, cytoplasm for TaHTMAR) were also observed. Virus-induced gene silencing (VIGS) demonstrated that both TaHGSNAT and TaHTMAR are essential for normal pollen development and anther dehiscence. Mechanistic studies using dual-luciferase reporter assays and GUS staining revealed that TaHTMAR functionally upregulates TaHGSNAT expression, suggesting a bidirectional positive feedback loop. Collectively, our findings define a TaHTMAR-TaHGSNAT regulatory module and provide new molecular insights into the genetic control of thermo-sensitive male fertility in wheat.

小麦温敏雄性不育是一种很有前途的杂交育种系统，但其育性转化的分子机制尚不明确。在K-TCMS系KTM3315A中，我们发现了一个基因对：TaHGSNAT（编码肝素-α-氨基葡萄糖n-乙酰转移酶）及其反义长链非编码RNA TaHTMAR。这两个转录本在花粉发育过程中同步共表达模式。不同的亚细胞定位（膜为tagsnat，细胞质为TaHTMAR）也被观察到。病毒诱导的基因沉默（VIGS）表明，TaHTMAR和tagsnat对正常花粉发育和花药裂解都是必需的。利用双荧光素酶报告基因检测和GUS染色进行的机制研究显示，TaHTMAR在功能上上调了TaHTMAR的表达，表明这是一个双向的正反馈回路。总之，我们的研究结果定义了一个TaHTMAR-TaHGSNAT调控模块，并为小麦温敏雄性育性的遗传控制提供了新的分子见解。

{"title":"A positive feedback loop between the lncRNA TaHTMAR and TaHGSNAT is essential for thermo-sensitive male fertility in wheat","authors":"Xiaoran Gao, Qiang Geng, Xin Che, Fuqiang Niu, Jinke Liu, Lingling Hu, Xiaoxia Wang, Xiyue Song, Lingli Zhang","doi":"10.1016/j.plantsci.2026.113022","DOIUrl":"10.1016/j.plantsci.2026.113022","url":null,"abstract":"<div><div>Wheat thermo-sensitive male sterility with <em>Aegilops kotschyi</em> cytoplasm (K-TCMS) is a promising system for hybrid breeding, yet the molecular mechanisms governing fertility conversion remain elusive. In the K-TCMS line KTM3315A, we identified a gene pair: <em>TaHGSNAT</em> (encoding a heparin-α-glucosaminide N-acetyltransferase) and its antisense long non-coding RNA, TaHTMAR. These two transcripts synchronized co-expression patterns during pollen development. Distinct subcellular localizations (membrane for <em>TaHGSNAT</em>, cytoplasm for TaHTMAR) were also observed. Virus-induced gene silencing (VIGS) demonstrated that both <em>TaHGSNAT</em> and TaHTMAR are essential for normal pollen development and anther dehiscence. Mechanistic studies using dual-luciferase reporter assays and GUS staining revealed that TaHTMAR functionally upregulates <em>TaHGSNAT</em> expression, suggesting a bidirectional positive feedback loop. Collectively, our findings define a TaHTMAR-<em>TaHGSNAT</em> regulatory module and provide new molecular insights into the genetic control of thermo-sensitive male fertility in wheat.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113022"},"PeriodicalIF":4.1,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146132990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An AT-hook motif nuclear protein AHL13 interacts with Poly(ADP-ribose) to regulate Arabidopsis immunity AT-hook基序核蛋白AHL13与Poly（adp -核糖）相互作用调节拟南芥免疫。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-02-03 DOI: 10.1016/j.plantsci.2026.113021

Jia Liu , Zhuo Chen , Ling Sun , Tingyan Huang , Yu Chen , Xiaohui Li , Hu Liu , Xinyi Huang , Yan Peng , Baomin Feng

Poly(ADP-ribosyl)ation or PARylation is required for immune transcription and defense against microbes in plants. However, the mechanisms underlying the PARylation-mediated transcriptional regulation are largely unknown. In this study, an AT-hook motif nuclear localized transcription factor, AHL13, was identified as an interactor of poly (ADP-ribose), the polymer products of PARylation. The knock-out and over-expression experiments suggest that AHL13 functions as a negative regulator of Arabidopsis immunity. RNA seq results showed that a group of defense-related genes were repressed by AHL13 upon bacterial infection. AHL13 could directly bind to the AT-rich sequences in the promoters of the target genes in the EMSA and MST assays. The PAR polymers directly interact with AHL13 with high affinity and significantly suppress its interaction with the AT-rich DNA, suggesting that PARylation might promote immune transcription through a repressor-repelling mechanism. In summary, this study revealed that the PAR-AHL13 interaction plays significant roles in immune gene expression in Arabidopsis.

Poly（adp -核糖基）化或PARylation是植物免疫转录和防御微生物所必需的。然而，parylation介导的转录调控机制在很大程度上是未知的。在这项研究中，一个AT-hook基序核定位转录因子AHL13被鉴定为聚磷酸基化的聚合物产物poly （adp -核糖）的相互作用因子。敲除和过表达实验表明，AHL13是拟南芥免疫的负调控因子。RNA测序结果显示，在细菌感染时，一组防御相关基因被AHL13抑制。在EMSA和MST实验中，AHL13可以直接结合靶基因启动子中富含at的序列。PAR聚合物直接与AHL13高亲和力相互作用，并显著抑制其与富含at的DNA的相互作用，提示PARylation可能通过抑制因子排斥机制促进免疫转录。综上所述，本研究揭示了PAR-AHL13相互作用在拟南芥免疫基因表达中发挥重要作用。

{"title":"An AT-hook motif nuclear protein AHL13 interacts with Poly(ADP-ribose) to regulate Arabidopsis immunity","authors":"Jia Liu , Zhuo Chen , Ling Sun , Tingyan Huang , Yu Chen , Xiaohui Li , Hu Liu , Xinyi Huang , Yan Peng , Baomin Feng","doi":"10.1016/j.plantsci.2026.113021","DOIUrl":"10.1016/j.plantsci.2026.113021","url":null,"abstract":"<div><div>Poly(ADP-ribosyl)ation or PARylation is required for immune transcription and defense against microbes in plants. However, the mechanisms underlying the PARylation-mediated transcriptional regulation are largely unknown. In this study, an AT-hook motif nuclear localized transcription factor, AHL13, was identified as an interactor of poly (ADP-ribose), the polymer products of PARylation. The knock-out and over-expression experiments suggest that AHL13 functions as a negative regulator of <em>Arabidopsis</em> immunity. RNA seq results showed that a group of defense-related genes were repressed by AHL13 upon bacterial infection. AHL13 could directly bind to the AT-rich sequences in the promoters of the target genes in the EMSA and MST assays. The PAR polymers directly interact with AHL13 with high affinity and significantly suppress its interaction with the AT-rich DNA, suggesting that PARylation might promote immune transcription through a repressor-repelling mechanism. In summary, this study revealed that the PAR-AHL13 interaction plays significant roles in immune gene expression in <em>Arabidopsis</em>.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113021"},"PeriodicalIF":4.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146126229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jasmonic acid affects epidermal cell fate determination via influencing cell cycle related gene expression in mangrove Avicennia marina 茉莉酸通过影响红树细胞周期相关基因的表达影响表皮细胞命运的决定。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-02-03 DOI: 10.1016/j.plantsci.2026.113019

Xingyue Hong , Hanchen Tang , Hezi Huang , Mingyue Wei , Mengqi Wu , Zhaoyu Guo , Jiakun Liu , Lihan Zhuang , Ling Sun , Jicheng Wang , Hanxin Zheng , Hai-Lei Zheng

Avicennia marina, a pioneer mangrove species, has adapted to the intertidal habitat along the tropical and subtropical coasts by developing salt glands on its leaf epidermis. Jasmonic acid (JA) is known to regulate the development of various plant epidermis. However, its role in the development of salt glands in A. marina remains unclear. In this study, we treated A. marina seedling using exogenous methyl jasmonate (MeJA) to investigate the effect of JA on the development and cell fate determination of salt glands, stomata and trichomes in A. marina leaf. The results showed MeJA significantly increased both the density of salt glands and the Na⁺ secretion. Besides, MeJA treatment positively regulated the trichome initiation and negatively affected stomatal lineage ground cells, with a significant decrease in stomatal density but no significant change in trichome density, while it exhibited that salt gland cells may partially originate from trichomes or stomatal lineage cells. Moreover, qRT-PCR results indicated that MeJA affects salt gland development via influencing the process of cell cycle, like reducing endoreduplication. These findings clarify how salt glands contribute to A. marina adaptation to coastal intertidal habitat from a tissue development perspective.

红树是红树林的先驱物种，它通过在叶表皮上发育盐腺来适应热带和亚热带海岸的潮间带生境。众所周知，茉莉酸（Jasmonic acid， JA）可以调节多种植物表皮的发育。然而，其在盐腺发育中的作用尚不清楚。本研究采用外源茉莉酸甲酯（MeJA）处理金银花幼苗，研究了茉莉酸甲酯对金银花叶片盐腺、气孔和毛状体发育及细胞命运的影响。结果表明，MeJA显著增加了盐腺密度和Na⁺的分泌量。此外，MeJA处理对毛状体起始有正向调节作用，对气孔系地细胞有负向影响，气孔密度显著降低，毛状体密度无显著变化，说明盐腺细胞可能部分来源于毛状体或气孔系细胞。此外，qRT-PCR结果表明，MeJA通过影响细胞周期过程，如减少内复制，影响盐腺发育。这些发现从组织发育的角度阐明了盐腺如何促进沙蚕适应沿海潮间带生境。

{"title":"Jasmonic acid affects epidermal cell fate determination via influencing cell cycle related gene expression in mangrove Avicennia marina","authors":"Xingyue Hong , Hanchen Tang , Hezi Huang , Mingyue Wei , Mengqi Wu , Zhaoyu Guo , Jiakun Liu , Lihan Zhuang , Ling Sun , Jicheng Wang , Hanxin Zheng , Hai-Lei Zheng","doi":"10.1016/j.plantsci.2026.113019","DOIUrl":"10.1016/j.plantsci.2026.113019","url":null,"abstract":"<div><div><em>Avicennia marina</em>, a pioneer mangrove species, has adapted to the intertidal habitat along the tropical and subtropical coasts by developing salt glands on its leaf epidermis. Jasmonic acid (JA) is known to regulate the development of various plant epidermis. However, its role in the development of salt glands in <em>A. marina</em> remains unclear. In this study, we treated <em>A. marina</em> seedling using exogenous methyl jasmonate (MeJA) to investigate the effect of JA on the development and cell fate determination of salt glands, stomata and trichomes in <em>A. marina</em> leaf. The results showed MeJA significantly increased both the density of salt glands and the Na⁺ secretion. Besides, MeJA treatment positively regulated the trichome initiation and negatively affected stomatal lineage ground cells, with a significant decrease in stomatal density but no significant change in trichome density, while it exhibited that salt gland cells may partially originate from trichomes or stomatal lineage cells. Moreover, qRT-PCR results indicated that MeJA affects salt gland development via influencing the process of cell cycle, like reducing endoreduplication. These findings clarify how salt glands contribute to <em>A. marina</em> adaptation to coastal intertidal habitat from a tissue development perspective.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113019"},"PeriodicalIF":4.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146126369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous plant growth-promoting rhizobacteria boosting photosynthetic efficiency via thylakoid lipid restructuring in potato under low-potassium conditions 低钾条件下外源植物促生根瘤菌通过类囊体脂质重组提高马铃薯光合效率

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-02-03 DOI: 10.1016/j.plantsci.2026.113012

Xin Zhou , Xin Cheng , Baoyu Fu , Meiling Li , Chao Luo , Haoyang Gong , Hongkun Yang , Jingye Fu , Chengcheng Cai , Kaiqin Zhang , Shunlin Zheng

To investigate novel pathways by which exogenous plant growth-promoting rhizobacteria (PGPR) alleviate potassium deficiency in crops, this study employed potato plants cultivated under a 50 % reduction in potassium fertilization as a model system. We elucidate the physiological and molecular mechanisms through which Enterobacter asburiae S13 optimizes photosynthetic performance via non-stomatal pathways. Application of E. asburiae S13 suspension significantly enhanced net photosynthetic rate and Rubisco activity while reducing intercellular CO₂ concentration without affecting stomatal conductance, confirming that photosynthetic enhancement is mediated through non-stomatal regulation. Chlorophyll a fluorescence kinetics (OJIP) and energy flux modeling revealed that bacterial treatment increased light-harvesting efficiency in photosystem II (PSII) while reducing energy dissipation. Integrated multi-omics analyses further identified glycerophospholipid metabolism as the core pathway, driving thylakoid membrane restructuring through upregulation of thylakoid structure-related genes and lipid metabolites. This ultimately restored tuber yield to levels comparable to conventional potassium fertilization under low-potassium conditions. Overall, this study proposes a novel paradigm in which exogenous PGPR enhances crop photosynthetic efficiency through a “membrane lipid metabolism → thylakoid optimization → PSII functional enhancement” cascade, providing a theoretical foundation for developing sustainable potassium-reduction technologies based on precision regulation of photosynthetic machinery.

为了探索外源植物促生根瘤菌（PGPR）缓解作物缺钾的新途径，本研究以钾肥减量50%条件下栽培的马铃薯为模型系统。我们阐明了asburiae肠杆菌S13通过非气孔途径优化光合性能的生理和分子机制。施用沙蚕S13悬浮液显著提高了净光合速率和Rubisco活性，降低了细胞间CO2浓度，但不影响气孔导度，证实了光合增强是通过非气孔调节介导的。叶绿素a荧光动力学（OJIP）和能量通量模型显示，细菌处理提高了光系统II （PSII）的光收集效率，同时减少了能量耗散。综合多组学分析进一步发现，甘油磷脂代谢是核心途径，通过上调类囊体结构相关基因和脂质代谢物，驱动类囊体膜重组。这最终使块茎产量恢复到与低钾条件下常规钾肥相当的水平。总体而言，本研究提出了外源PGPR通过“膜脂代谢→类囊体优化→PSII功能增强”级联提高作物光合效率的新范式，为开发基于光合机制精确调控的可持续钾还原技术提供了理论基础。

{"title":"Exogenous plant growth-promoting rhizobacteria boosting photosynthetic efficiency via thylakoid lipid restructuring in potato under low-potassium conditions","authors":"Xin Zhou , Xin Cheng , Baoyu Fu , Meiling Li , Chao Luo , Haoyang Gong , Hongkun Yang , Jingye Fu , Chengcheng Cai , Kaiqin Zhang , Shunlin Zheng","doi":"10.1016/j.plantsci.2026.113012","DOIUrl":"10.1016/j.plantsci.2026.113012","url":null,"abstract":"<div><div>To investigate novel pathways by which exogenous plant growth-promoting rhizobacteria (PGPR) alleviate potassium deficiency in crops, this study employed potato plants cultivated under a 50 % reduction in potassium fertilization as a model system. We elucidate the physiological and molecular mechanisms through which <em>Enterobacter asburiae</em> S13 optimizes photosynthetic performance via non-stomatal pathways. Application of <em>E. asburiae</em> S13 suspension significantly enhanced net photosynthetic rate and Rubisco activity while reducing intercellular CO<sub>2</sub> concentration without affecting stomatal conductance, confirming that photosynthetic enhancement is mediated through non-stomatal regulation. Chlorophyll a fluorescence kinetics (OJIP) and energy flux modeling revealed that bacterial treatment increased light-harvesting efficiency in photosystem II (PSII) while reducing energy dissipation. Integrated multi-omics analyses further identified glycerophospholipid metabolism as the core pathway, driving thylakoid membrane restructuring through upregulation of thylakoid structure-related genes and lipid metabolites. This ultimately restored tuber yield to levels comparable to conventional potassium fertilization under low-potassium conditions. Overall, this study proposes a novel paradigm in which exogenous PGPR enhances crop photosynthetic efficiency through a “membrane lipid metabolism → thylakoid optimization → PSII functional enhancement” cascade, providing a theoretical foundation for developing sustainable potassium-reduction technologies based on precision regulation of photosynthetic machinery.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113012"},"PeriodicalIF":4.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146126220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing the rhizosphere to enhance photosynthesis and functional traits of Cyperus esculentus in saline-alkali soils: A comparison between biochemical amendments and salt-tolerant plant intercropping 盐碱土壤根际优化提高沙柏光合和功能性状：生化改良与耐盐作物间作的比较

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-02-03 DOI: 10.1016/j.plantsci.2026.113018

Xin Shen , Weiyi Zhou , Xiangyi Li , Yalan Liu , Bixia Nie

Soil salinization is a critical barrier to agriculture, especially in arid regions like Xinjiang, where it restricts crop growth. Cyperus esculentus, a moderately salt-tolerant oilseed crop with ecological and economic value, faces cultivation challenges under high saline-alkali soils. Although biological strategies have been proposed to alleviate salt stress, their effects on the crop’s photosynthetic performance remain insufficiently studied. This study evaluated two approaches—biochemical amendments and intercropping with salt-tolerant plants—to assess their impact on soil properties and functional traits of C. esculentus. Microbial agents and humic acid improved the rhizosphere by reducing soil pH by 10–14 % and increasing available nitrogen by 29–51 %, thereby enhancing chlorophyll a synthesis and leaf nitrogen content. By lowering the salt content of the soil and possibly boosting beneficial microbial activity, intercropping enhanced the root environment. It increased net photosynthetic rates by 20–22 % and suggested better electron transfer. Through root interactions with companion plants, nutrient uptake was further enhanced. In comparison to CK, both treatments greatly boosted biomass, with leaf biomass increasing by 18–27 % at maturity. Although our data point to distinct primary pathways, intercropping was mainly linked to synergistic optimization of morphology and photosystem electron transport, whereas biochemical amendments were linked to improved nutrient status and photosynthetic efficiency, suggesting potential physiological stability. These findings provide insight into the physiological adaptation of C. esculentus under salinity and offer practical guidance for its sustainable cultivation in saline-alkali soils.

土壤盐碱化是农业的一个重要障碍，特别是在新疆等干旱地区，它限制了作物的生长。沙菖蒲是一种具有生态经济价值的中等耐盐油料作物，在高盐碱土壤条件下面临着栽培挑战。虽然已经提出了减轻盐胁迫的生物策略，但它们对作物光合性能的影响仍未得到充分研究。本文评价了生化改良和耐盐植物间作两种改良方法对甜瓜土壤性质和功能性状的影响。微生物剂和腐植酸改善根际土壤，使土壤pH降低10-14%，速效氮增加29-51%，从而提高叶绿素a合成和叶片氮含量。间作通过降低土壤含盐量并可能提高有益微生物的活性，改善了根系环境。它使净光合速率提高了20-22%，并显示出更好的电子转移。通过根系与伴生植物的相互作用，进一步促进了养分的吸收。与对照相比，两种处理均显著提高了叶片生物量，成熟期叶片生物量提高了18-27%。虽然我们的数据指向不同的主要途径，但间作主要与形态和光系统电子传递的协同优化有关，而生化修饰与营养状况和光合效率的改善有关，表明潜在的生理稳定性。这些研究结果有助于深入了解青花在盐碱地的生理适应性，为青花在盐碱地的可持续栽培提供实践指导。

{"title":"Optimizing the rhizosphere to enhance photosynthesis and functional traits of Cyperus esculentus in saline-alkali soils: A comparison between biochemical amendments and salt-tolerant plant intercropping","authors":"Xin Shen , Weiyi Zhou , Xiangyi Li , Yalan Liu , Bixia Nie","doi":"10.1016/j.plantsci.2026.113018","DOIUrl":"10.1016/j.plantsci.2026.113018","url":null,"abstract":"<div><div>Soil salinization is a critical barrier to agriculture, especially in arid regions like Xinjiang, where it restricts crop growth. <em>Cyperus esculentus</em>, a moderately salt-tolerant oilseed crop with ecological and economic value, faces cultivation challenges under high saline-alkali soils. Although biological strategies have been proposed to alleviate salt stress, their effects on the crop’s photosynthetic performance remain insufficiently studied. This study evaluated two approaches—biochemical amendments and intercropping with salt-tolerant plants—to assess their impact on soil properties and functional traits of <em>C. esculentus</em>. Microbial agents and humic acid improved the rhizosphere by reducing soil pH by 10–14 % and increasing available nitrogen by 29–51 %, thereby enhancing chlorophyll a synthesis and leaf nitrogen content. By lowering the salt content of the soil and possibly boosting beneficial microbial activity, intercropping enhanced the root environment. It increased net photosynthetic rates by 20–22 % and suggested better electron transfer. Through root interactions with companion plants, nutrient uptake was further enhanced. In comparison to CK, both treatments greatly boosted biomass, with leaf biomass increasing by 18–27 % at maturity. Although our data point to distinct primary pathways, intercropping was mainly linked to synergistic optimization of morphology and photosystem electron transport, whereas biochemical amendments were linked to improved nutrient status and photosynthetic efficiency, suggesting potential physiological stability. These findings provide insight into the physiological adaptation of <em>C. esculentus</em> under salinity and offer practical guidance for its sustainable cultivation in saline-alkali soils.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113018"},"PeriodicalIF":4.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146126445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyploidization disrupts drought response and epigenetic patterns in the desert wild potato species Solanum kurtzianum 沙漠野生马铃薯的多倍体化破坏了干旱响应和表观遗传模式。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-02-02 DOI: 10.1016/j.plantsci.2026.113017

Damián N. Jerez , Carina V. González , Perla C. Kozub , Verónica N. Ibañez , Federico Berli , Ricardo W. Masuelli , Carlos F. Marfil

Polyploidy, the possession of more than two sets of chromosomes in a cell, is often linked to enhanced adaptability and stress resilience in plants, though it may introduce genomic instability and fitness costs. Here, we examined the interplay between ploidy level and epigenetic responses to drought stress in Solanum kurtzianum, a drought-tolerant wild relative of potato. We subjected diploid and newly oryzalin-induced autotetraploid lines to three different watering regimes and assessed their morphological, physiological, biochemical, and epigenetic responses. Diploids consistently outperformed autotetraploids in drought tolerance and tuber yield under moderate water stress, exhibiting lower stomatal conductance, yet maintaining comparable photochemical efficiency. Epigenetic analyses revealed significant interplay between the ploidy level and drought conditions on methylation patterns, with autotetraploids displaying higher methylation variability and greater genomic instability under severe drought. These findings are consistent with the predominance of diploid wild potatoes in arid regions and suggest that genomic instability caused by polyploidy may compromise drought resilience. The study emphasizes the adaptive potential of diploid wild potatoes in arid environments and the role of epigenetic mechanisms in stress responses. Our results have implications for potato breeding strategies, highlighting the potential of diploids for developing drought-resilient cultivars to cope with climate change challenges.

多倍体，即在一个细胞中拥有两组以上的染色体，通常与植物增强的适应性和抗逆性有关，尽管它可能会带来基因组的不稳定性和适应性成本。本研究研究了马铃薯的耐旱野生亲缘植物茄（Solanum kurtzianum）的倍性水平与表观遗传对干旱胁迫的反应之间的相互作用。我们将二倍体和新稻杂素诱导的同源四倍体系置于三种不同的浇水制度下，并评估了它们的形态、生理、生化和表观遗传反应。在中等水分胁迫下，二倍体在抗旱性和块茎产量方面始终优于同源四倍体，表现出较低的气孔导度，但保持相当的光化学效率。表观遗传分析显示，倍性水平和干旱条件对甲基化模式有显著的相互作用，同源四倍体在严重干旱条件下表现出更高的甲基化变异性和更大的基因组不稳定性。这些发现与二倍体野生马铃薯在干旱地区的优势一致，表明多倍体引起的基因组不稳定性可能会损害抗旱能力。本研究强调了二倍体野生马铃薯在干旱环境中的适应潜力以及表观遗传机制在逆境响应中的作用。我们的研究结果对马铃薯育种策略具有启示意义，突出了二倍体在开发抗旱品种以应对气候变化挑战方面的潜力。

{"title":"Polyploidization disrupts drought response and epigenetic patterns in the desert wild potato species Solanum kurtzianum","authors":"Damián N. Jerez , Carina V. González , Perla C. Kozub , Verónica N. Ibañez , Federico Berli , Ricardo W. Masuelli , Carlos F. Marfil","doi":"10.1016/j.plantsci.2026.113017","DOIUrl":"10.1016/j.plantsci.2026.113017","url":null,"abstract":"<div><div>Polyploidy, the possession of more than two sets of chromosomes in a cell, is often linked to enhanced adaptability and stress resilience in plants, though it may introduce genomic instability and fitness costs. Here, we examined the interplay between ploidy level and epigenetic responses to drought stress in <em>Solanum kurtzianum</em>, a drought-tolerant wild relative of potato. We subjected diploid and newly oryzalin-induced autotetraploid lines to three different watering regimes and assessed their morphological, physiological, biochemical, and epigenetic responses. Diploids consistently outperformed autotetraploids in drought tolerance and tuber yield under moderate water stress, exhibiting lower stomatal conductance, yet maintaining comparable photochemical efficiency. Epigenetic analyses revealed significant interplay between the ploidy level and drought conditions on methylation patterns, with autotetraploids displaying higher methylation variability and greater genomic instability under severe drought. These findings are consistent with the predominance of diploid wild potatoes in arid regions and suggest that genomic instability caused by polyploidy may compromise drought resilience. The study emphasizes the adaptive potential of diploid wild potatoes in arid environments and the role of epigenetic mechanisms in stress responses. Our results have implications for potato breeding strategies, highlighting the potential of diploids for developing drought-resilient cultivars to cope with climate change challenges.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113017"},"PeriodicalIF":4.1,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146119616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of Kongyu 131 mutant library provides genetic resources for rice functional genomics and germplasm improvement 空育131突变文库的构建为水稻功能基因组学和种质改良提供遗传资源

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-01-31 DOI: 10.1016/j.plantsci.2026.113016

Tingting Xu , Qun Yang , Ran Li , Jianping Wang , Zhishan Luo , Daoyong Gong , Leihao Weng , Xiaoshan Huang , Debao Fu , Jing Li

Kongyu 131 (Oryza sativa ssp. japonica) is an elite rice cultivar widely planted in Heilongjiang Province, China. Here, we subjected Kongyu 131 to ethyl methanesulfonate (EMS)-induced mutagenesis to establish a genome-wide mutant library. On this basis, we generated 8770 M2 lines, and systematically screened 2215 lines with 42 distinct phenotypic variations across all developmental stages. Then, we employed a three-dimensional (3D) pooling strategy to combine 6144 M3 DNA samples into 224 multiplexed pools. By using the TILLING-seq (Targeting Induced Local Lesions in Genomes sequencing) approach, we identified 84 mutants across five key genes (GS3, Pi21, CSA, OsRR22, and OsaTRZ2), and validated their allelic variations. Phenotypic screening generated 18 lines with superior plant architecture, including a novel OsSPL14 allele identified via MutMap+ analysis. Whole-genome sequencing (WGS) of three M2 lines and TILLING-seq of mutants of the five key genes demonstrated a high mutation density (1 mutation per 121–288 kb). This 3D-pooled and genome-saturated EMS mutant library represents a robust resource for advancing functional genomic studies and precision breeding in rice.

空玉131 （Oryza sativa ssp）粳稻（japonica）是中国黑龙江省广泛种植的优良水稻品种。在此，我们对空玉131进行了甲基磺酸乙酯（EMS）诱变，建立了全基因组突变文库。在此基础上，我们获得了8770个M2株系，并系统筛选了2215个株系，在所有发育阶段有42个不同的表型变异。然后，我们采用三维（3D）池策略将6144 M3 DNA样本组合到224个多路池中。通过TILLING-seq （Targeting Induced Local lesion in Genomes sequencing）方法，我们鉴定了5个关键基因（GS3、Pi21、CSA、OsRR22和OsaTRZ2）中的84个突变体，并验证了它们的等位基因变异。表型筛选产生了18个具有优良植株结构的株系，其中包括一个通过MutMap+分析鉴定出的新的OsSPL14等位基因。3个M2系的全基因组测序和5个关键基因突变体的tillling -seq结果显示，突变密度较高（每121-288kb有1个突变）。这个3d池和基因组饱和的EMS突变文库为推进水稻功能基因组研究和精确育种提供了强大的资源。

{"title":"Construction of Kongyu 131 mutant library provides genetic resources for rice functional genomics and germplasm improvement","authors":"Tingting Xu , Qun Yang , Ran Li , Jianping Wang , Zhishan Luo , Daoyong Gong , Leihao Weng , Xiaoshan Huang , Debao Fu , Jing Li","doi":"10.1016/j.plantsci.2026.113016","DOIUrl":"10.1016/j.plantsci.2026.113016","url":null,"abstract":"<div><div>Kongyu 131 (<em>Oryza sativa</em> ssp. <em>japonica</em>) is an elite rice cultivar widely planted in Heilongjiang Province, China. Here, we subjected Kongyu 131 to ethyl methanesulfonate (EMS)-induced mutagenesis to establish a genome-wide mutant library. On this basis, we generated 8770 M2 lines, and systematically screened 2215 lines with 42 distinct phenotypic variations across all developmental stages. Then, we employed a three-dimensional (3D) pooling strategy to combine 6144 M3 DNA samples into 224 multiplexed pools. By using the TILLING-seq (Targeting Induced Local Lesions in Genomes sequencing) approach, we identified 84 mutants across five key genes (<em>GS3</em>, <em>Pi21</em>, <em>CSA</em>, <em>OsRR22</em>, and <em>OsaTRZ2</em>), and validated their allelic variations. Phenotypic screening generated 18 lines with superior plant architecture, including a novel <em>OsSPL14</em> allele identified via MutMap+ analysis. Whole-genome sequencing (WGS) of three M2 lines and TILLING-seq of mutants of the five key genes demonstrated a high mutation density (1 mutation per 121–288 kb). This 3D-pooled and genome-saturated EMS mutant library represents a robust resource for advancing functional genomic studies and precision breeding in rice.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113016"},"PeriodicalIF":4.1,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146107015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic analysis identifies VvMYB90 and VvNAC92 as key regulators mediating anthocyanin biosynthesis and fruit color differentiation in three grape cultivars 转录组学分析发现，VvMYB90和VvNAC92是3个葡萄品种花青素生物合成和果实颜色分化的关键调控因子。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-01-30 DOI: 10.1016/j.plantsci.2026.113009

Han Lin , Kaili Mao , Shilong Chai, Jiangmin Lan, Hongbo Xiao, Zhixiong Guo, Tengfei Pan, Wenqin She

‘Shine Muscat’ (YG), ‘Gaoqiansui’ (GQS), and ‘Moldova’ (ME) are three grape cultivars with distinct phenotypic differences in fruits, serving as ideal materials for deciphering the mechanism of grape color differentiation. Currently, the specific mechanisms underlying the regulation of anthocyanin synthesis and color differentiation during grape fruit development require further investigation. To understand these, transcriptomic analysis was performed in this study to compare the different developmental stages of the three grape cultivars. Transcriptomic analysis displayed significant enrichment of differentially expressed genes (DEGs) in the phenylpropanoid and flavonoid biosynthesis pathways. These pathway-related genes exhibited a significant upregulation in expression during the veraison and maturation stages in the GQS and ME groups, with the most prominent upregulation observed in the ME group. Meanwhile, WGCNA and correlation network heatmap were employed to construct a TF-structural gene regulatory network associated with grape anthocyanin synthesis. VvMYB90 may mediate the regulation of gene-UFGT, whereas VvNAC92 may engage in the regulation of seven structural genes (gene-CHS, gene-GST4, gene-LOC100250360, gene-LOC100250579, gene-LOC100255217, gene-LOC100261962, and gene-UFGT). Collectively, these findings reveal the molecular basis of anthocyanin biosynthesis and color differentiation in grape fruits, providing meaningful insights into the accurate regulation of grape peel color.

“Shine Muscat”（YG）、“高干穗”（GQS）和“Moldova”（ME）是三个果实表型差异明显的葡萄品种，是研究葡萄颜色分化机制的理想材料。目前，葡萄果实发育过程中花青素合成和颜色分化调控的具体机制有待进一步研究。为了了解这些，本研究进行了转录组学分析，比较了三个葡萄品种的不同发育阶段。转录组学分析显示，在苯丙素和类黄酮生物合成途径中，差异表达基因（DEGs）显著富集。这些通路相关基因在GQS组和ME组的变异和成熟阶段均有显著的表达上调，其中ME组的表达上调最为显著。同时，利用WGCNA和相关网络热图构建葡萄花青素合成相关tf结构基因调控网络。VvMYB90可能介导- ufgt基因的调控，而VvNAC92可能参与7个结构基因（基因- chs、基因- gst4、基因- loc100250360、基因- loc100250579、基因- loc100255217、基因- loc100261962和基因- ufgt）的调控。总之，这些发现揭示了葡萄果实花青素生物合成和颜色分化的分子基础，为葡萄果皮颜色的准确调控提供了有意义的见解。

{"title":"Transcriptomic analysis identifies VvMYB90 and VvNAC92 as key regulators mediating anthocyanin biosynthesis and fruit color differentiation in three grape cultivars","authors":"Han Lin , Kaili Mao , Shilong Chai, Jiangmin Lan, Hongbo Xiao, Zhixiong Guo, Tengfei Pan, Wenqin She","doi":"10.1016/j.plantsci.2026.113009","DOIUrl":"10.1016/j.plantsci.2026.113009","url":null,"abstract":"<div><div>‘Shine Muscat’ (YG), ‘Gaoqiansui’ (GQS), and ‘Moldova’ (ME) are three grape cultivars with distinct phenotypic differences in fruits, serving as ideal materials for deciphering the mechanism of grape color differentiation. Currently, the specific mechanisms underlying the regulation of anthocyanin synthesis and color differentiation during grape fruit development require further investigation. To understand these, transcriptomic analysis was performed in this study to compare the different developmental stages of the three grape cultivars. Transcriptomic analysis displayed significant enrichment of differentially expressed genes (DEGs) in the phenylpropanoid and flavonoid biosynthesis pathways. These pathway-related genes exhibited a significant upregulation in expression during the veraison and maturation stages in the GQS and ME groups, with the most prominent upregulation observed in the ME group. Meanwhile, WGCNA and correlation network heatmap were employed to construct a TF-structural gene regulatory network associated with grape anthocyanin synthesis. VvMYB90 may mediate the regulation of gene-UFGT, whereas VvNAC92 may engage in the regulation of seven structural genes (gene-CHS, gene-GST4, gene-LOC100250360, gene-LOC100250579, gene-LOC100255217, gene-LOC100261962, and gene-UFGT). Collectively, these findings reveal the molecular basis of anthocyanin biosynthesis and color differentiation in grape fruits, providing meaningful insights into the accurate regulation of grape peel color.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113009"},"PeriodicalIF":4.1,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0