Plant Science最新文献_第2页

GmEXPA11 facilitates nodule enlargement and nitrogen fixation via interaction with GmNOD20 under regulation of GmPTF1 in soybean

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-03-10 DOI: 10.1016/j.plantsci.2025.112469

Xinzhu Xing , Hui Du , Zhanwu Yang , Hua Zhang , Na Li , Zhenqi Shao , Wenlong Li , Youbin Kong , Xihuan Li , Caiying Zhang

Biological nitrogen fixation (BNF) provides 50–60 % of the nitrogen for plant growth and development, while its application is restricted for the deficiency of functional gene in biological breeding. Expansin can enlarge the plant cells through loosening the cell wall, which has a great breeding potential for legumes BNF improvement. In the present study, a cell wall α-subfamily expansin, GmEXPA11, was isolated and analyzed in soybean nodule growth and nitrogen fixation process. GmEXPA11 was highly induced by rhizobial infection and appeared high expressions in the whole process of soybean nodulation and nitrogen fixation. The overexpression of GmEXPA11 facilitated nodule cell enlargement and generated much more big nodules, with an increase of 37.6 % on nodule cell length, 14.7 % on cell width, 25.8 % on big nodule number, 25.6 % on nodule weight, while the RNAi nodules were opposite. Moreover, GmEXPA11 overexpression enhanced nodule nitrogen fixation ability, with the increases of 22.9 %, 6.7 % and 11.7 % on nitrogenase activity, nitrogen content and hairy root nitrogen content, while the RNAi decreased by 11.9 %, 10.7 % and 7.8 %, respectively. Further analysis demonstrated that GmEXPA11 affected nodules enlargement and nitrogen fixation via interacting with nodulin GmNOD20 under the regulation of transcription factor GmPTF1. The expression of GmEXPA11 was significantly increased in the transgenic nodules with GmPTF1 over-expressed. In addition, by analyzing soybean resequencing accessions, four upstream SNPs were found in the promoter of GmEXPA11 and formed two haplotypes with significantly different soybean nodulation and nitrogen fixation characters, which demonstrated the close relationship between GmEXPA11-SNPs and BNF.

{"title":"GmEXPA11 facilitates nodule enlargement and nitrogen fixation via interaction with GmNOD20 under regulation of GmPTF1 in soybean","authors":"Xinzhu Xing , Hui Du , Zhanwu Yang , Hua Zhang , Na Li , Zhenqi Shao , Wenlong Li , Youbin Kong , Xihuan Li , Caiying Zhang","doi":"10.1016/j.plantsci.2025.112469","DOIUrl":"10.1016/j.plantsci.2025.112469","url":null,"abstract":"<div><div>Biological nitrogen fixation (BNF) provides 50–60 % of the nitrogen for plant growth and development, while its application is restricted for the deficiency of functional gene in biological breeding. Expansin can enlarge the plant cells through loosening the cell wall, which has a great breeding potential for legumes BNF improvement. In the present study, a cell wall α-subfamily expansin, GmEXPA11, was isolated and analyzed in soybean nodule growth and nitrogen fixation process. GmEXPA11 was highly induced by rhizobial infection and appeared high expressions in the whole process of soybean nodulation and nitrogen fixation. The overexpression of GmEXPA11 facilitated nodule cell enlargement and generated much more big nodules, with an increase of 37.6 % on nodule cell length, 14.7 % on cell width, 25.8 % on big nodule number, 25.6 % on nodule weight, while the RNAi nodules were opposite. Moreover, GmEXPA11 overexpression enhanced nodule nitrogen fixation ability, with the increases of 22.9 %, 6.7 % and 11.7 % on nitrogenase activity, nitrogen content and hairy root nitrogen content, while the RNAi decreased by 11.9 %, 10.7 % and 7.8 %, respectively. Further analysis demonstrated that GmEXPA11 affected nodules enlargement and nitrogen fixation via interacting with nodulin GmNOD20 under the regulation of transcription factor GmPTF1. The expression of GmEXPA11 was significantly increased in the transgenic nodules with GmPTF1 over-expressed. In addition, by analyzing soybean resequencing accessions, four upstream SNPs were found in the promoter of GmEXPA11 and formed two haplotypes with significantly different soybean nodulation and nitrogen fixation characters, which demonstrated the close relationship between GmEXPA11-SNPs and BNF.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112469"},"PeriodicalIF":4.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional identification of mango MiGID1A and MiGID1B genes confers early flowering and stress tolerance

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-03-10 DOI: 10.1016/j.plantsci.2025.112468

Ruoyan Li , Cong Luo , Junjie Zhong, Yuan Liu, Huibao Wen, Fang Xu, Zhixi He, Chuting Huang, Xinhua He

The GIBBERELLIN INSENSITIVE DWARF1 (GID1) gene encodes a receptor integral to Gibberellic acid (GA) signaling, which is pivotal for plant growth, development, and stress responses. Until now, GID1 genes have not been documented in mango. In this research, the mango (Mangifera indica) genome yielded four GID1 homologous genes, and this study focuses on the research of MiGID1A and MiGID1B genes. Expression analysis indicated that MiGID1A is mainly expressed in leaves, while MiGID1B is predominantly found in flowers and buds. Both genes exhibited a significant upsurge in expression under salt and drought stress conditions. Moreover, the overexpression of these genes significantly advanced early flowering under long-day conditions. MiGID1A and MiGID1B transgenic plants showed significantly higher root length and survival rate than WT plants under drought and salt stress treatment. In addition, under drought and salt stress treatment, the contents of malonaldehyde (MAD) and hydrogen peroxide (H₂O₂) decreased significantly, and the levels of proline (Pro) and superoxide dismutase (SOD) notably increased in the MiGID1A-OE and MiGID1B-OE transgenic plants. GA₃ treatment significantly improved germination rates, root elongation, and early flowering in both MiGID1A-OE and MiGID1B-OE lines. At the same time, ABA treatment alleviated the inhibition of seed germination, root growth, and flowering in transgenic Arabidopsis. Moreover, yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays demonstrated that MiGID1A and MiGID1B were capable of interacting with DELLA family proteins. Existing reports have demonstrated that GID1 participates in various regulatory processes by promoting the degradation of DELLA proteins. SQUAMOSA promoter binding protein-like (SPL3a/b) and WRKY12a/b. The findings imply a significant regulatory function for the MiGID1A and MiGID1B genes in the processes of flowering, stress management, and gibberellin response.

Key message

MiGID1 as a GA receptor plays a critical role in the function of hormones, stress response, and promoting plant flowering.

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引用次数: 0

Effect of the overexpression of the GGP1 gene on cell wall remodelling and redox state in the tomato fruit

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-03-10 DOI: 10.1016/j.plantsci.2025.112470

Nataliia Kutyrieva-Nowak , Ana Pantelić , Stefan Isaković , Angelos K. Kanellis , Marija Vidović , Agata Leszczuk

Tomato fruit ripening is a complex physiological process that involves morphological, anatomical, biochemical, and molecular alterations. One of these changes occurring during ripening is the softening of the fruit, which is attributed to modifications in the biosynthesis and degradation of individual cell wall components, i.e. polysaccharides and proteoglycans. In addition, ripening is affected by redox processes, and interplay of the reactive oxygen species (ROS) and specific antioxidants, enzymes, ascorbate, and phenolic compounds. The present study aims to determine the impact of the overexpression of the GDP-l-galactose phosphorylase (GGP1) gene under the control of two fruit-specific promoters, namely PPC - phosphoenolpyruvate carboxylase and PG - polygalacturonase on cell wall properties, activities of H₂O₂-regulating enzymes and the abundance of phenolic compounds. PPC-GGP1 and PG-GGP1 transgenic lines revealed significant structural changes in fruit parenchyma, compared to wild type fruit, followed by a disturbance in the spatial distribution and molecular & chemical composition of homogalacturonans. In addition, cell wall-bound monolignol, p-coumaryl alcohol was higher in transgenic fruit compared with wild type ones. Lastly, the catalase and ascorbate peroxidase activities were lower in PPC-GGP1 fruits, indicating changes in the regulation of antioxidative defense during the ripening process of this line. These results suggest that overexpression of the GGP1 gene affects the cell wall remodelling and redox state in the red ripe tomato fruits.

{"title":"Effect of the overexpression of the GGP1 gene on cell wall remodelling and redox state in the tomato fruit","authors":"Nataliia Kutyrieva-Nowak , Ana Pantelić , Stefan Isaković , Angelos K. Kanellis , Marija Vidović , Agata Leszczuk","doi":"10.1016/j.plantsci.2025.112470","DOIUrl":"10.1016/j.plantsci.2025.112470","url":null,"abstract":"<div><div>Tomato fruit ripening is a complex physiological process that involves morphological, anatomical, biochemical, and molecular alterations. One of these changes occurring during ripening is the softening of the fruit, which is attributed to modifications in the biosynthesis and degradation of individual cell wall components, i.e. polysaccharides and proteoglycans. In addition, ripening is affected by redox processes, and interplay of the reactive oxygen species (ROS) and specific antioxidants, enzymes, ascorbate, and phenolic compounds. The present study aims to determine the impact of the overexpression of the GDP-l-galactose phosphorylase (GGP1) gene under the control of two fruit-specific promoters, namely PPC - phosphoenolpyruvate carboxylase and PG - polygalacturonase on cell wall properties, activities of H2O2-regulating enzymes and the abundance of phenolic compounds. PPC-GGP1 and PG-GGP1 transgenic lines revealed significant structural changes in fruit parenchyma, compared to wild type fruit, followed by a disturbance in the spatial distribution and molecular & chemical composition of homogalacturonans. In addition, cell wall-bound monolignol, p-coumaryl alcohol was higher in transgenic fruit compared with wild type ones. Lastly, the catalase and ascorbate peroxidase activities were lower in PPC-GGP1 fruits, indicating changes in the regulation of antioxidative defense during the ripening process of this line. These results suggest that overexpression of the GGP1 gene affects the cell wall remodelling and redox state in the red ripe tomato fruits.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112470"},"PeriodicalIF":4.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the agronomic performance of high-amylose durum wheat

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-03-08 DOI: 10.1016/j.plantsci.2025.112459

Arianna Frittelli , Ermelinda Botticella , Samuela Palombieri , Giulio Metelli , Stefania Masci , Marco Silvestri , Domenico Lafiandra , Francesco Sestili

High-amylose wheat has garnered significant attention from the food industry for its potential to produce low-glycaemic food products. It is well-established that there is a direct correlation between the amylose content in flour and the amount of resistant starch (RS) in foods.

Recently, some research initiatives have successfully produced high-amylose durum wheat by targeting key enzymes in the amylopectin biosynthesis pathway, though this has resulted in a reduction in seed weight. This study aimed to develop durum wheat genotypes with enhanced nutritional and agronomic traits by pyramiding mutations in the SSIIa genes and the GW2-A1 null allele. A cross between Svevo SSIIa^- and Kronos GW2-A1^- was performed, and marker-assisted selection (MAS) strategies were employed to identify ten sister lines (GW2-A1^-/SSIIa^-). Biochemical analyses revealed that the GW2-A1^-/SSIIa^- genotypes exhibited significantly higher amylose and resistant starch (5–10-fold) levels compared to Svevo and GW2-A1^- controls. Phenotypic analyses highlighted that GW2-A1^-/SSIIa^- lines showed a 50 % increase in hundred-grain weight (HGW) and improved grain size parameters compared to Svevo SSIIa^-, though these values remained lower than Svevo and Kronos GW2-A1^-. Yield per plot increased by 67 % compared to Svevo SSIIa^- but was 30–40 % lower than Svevo and Kronos GW2-A1^-. Gene expression analysis revealed upregulation of key starch biosynthesis genes (Susy2, UGPase) in GW2-A1^-/SSIIa^- lines, suggesting compensatory mechanisms for reduced starch content. Downregulation of TPS7 indicated potential limitations in trehalose-6-phosphate biosynthesis, which may influence starch accumulation. This study demonstrates that combining SSIIa and GW2-A1 null mutations can mitigate yield losses associated with high-amylose genotypes while maintaining elevated levels of resistant starch and dietary fiber.

{"title":"Improving the agronomic performance of high-amylose durum wheat","authors":"Arianna Frittelli , Ermelinda Botticella , Samuela Palombieri , Giulio Metelli , Stefania Masci , Marco Silvestri , Domenico Lafiandra , Francesco Sestili","doi":"10.1016/j.plantsci.2025.112459","DOIUrl":"10.1016/j.plantsci.2025.112459","url":null,"abstract":"<div><div>High-amylose wheat has garnered significant attention from the food industry for its potential to produce low-glycaemic food products. It is well-established that there is a direct correlation between the amylose content in flour and the amount of resistant starch (RS) in foods.</div><div>Recently, some research initiatives have successfully produced high-amylose durum wheat by targeting key enzymes in the amylopectin biosynthesis pathway, though this has resulted in a reduction in seed weight. This study aimed to develop durum wheat genotypes with enhanced nutritional and agronomic traits by pyramiding mutations in the SSIIa genes and the GW2-A1 null allele. A cross between Svevo SSIIa- and Kronos GW2-A1- was performed, and marker-assisted selection (MAS) strategies were employed to identify ten sister lines (GW2-A1-/SSIIa-). Biochemical analyses revealed that the GW2-A1-/SSIIa- genotypes exhibited significantly higher amylose and resistant starch (5–10-fold) levels compared to Svevo and GW2-A1- controls. Phenotypic analyses highlighted that GW2-A1-/SSIIa- lines showed a 50 % increase in hundred-grain weight (HGW) and improved grain size parameters compared to Svevo SSIIa-, though these values remained lower than Svevo and Kronos GW2-A1-. Yield per plot increased by 67 % compared to Svevo SSIIa- but was 30–40 % lower than Svevo and Kronos GW2-A1-. Gene expression analysis revealed upregulation of key starch biosynthesis genes (Susy2, UGPase) in GW2-A1-/SSIIa- lines, suggesting compensatory mechanisms for reduced starch content. Downregulation of TPS7 indicated potential limitations in trehalose-6-phosphate biosynthesis, which may influence starch accumulation. This study demonstrates that combining SSIIa and GW2-A1 null mutations can mitigate yield losses associated with high-amylose genotypes while maintaining elevated levels of resistant starch and dietary fiber.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112459"},"PeriodicalIF":4.2,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryptochromes (CRYs) in pepper: Genome-wide identification, evolution and functional analysis of the negative role of CaCRY1 under Phytophthora capsici infection

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-03-06 DOI: 10.1016/j.plantsci.2025.112460

Fei Sun , Yue Chen , Ying Luo , Feng Yang , Ting Yu , Huibin Han , Youxin Yang , Yong Zhou

Cryptochromes (CRYs) are ultraviolet-A (UV-A) and blue light photoreceptors that perceive UV-A and blue light to mediate a range of physiological processes including disease response in plants. However, there has been no report about the roles of CRY genes in pepper, which often suffers from Phytophthora blight caused by Phytophthora capsici. In this work, three pepper CRY genes were identified and their characteristics were examined by bioinformatics analysis. CaCRY1 is an ortholog of AtCRY1 located in the cytoplasm and nucleus, and expression analysis by RT-qPCR showed that its transcription was differentially regulated by jasmonic acid (JA) and salicylic acid (SA), as well as by P. capsici infection (PCI). Overexpression of CaCRY1 in pepper and Nicotiana benthamiana promoted the susceptibility of plants to PCI. Further virus-induced gene silencing (VIGS) analysis showed that silencing of CaCRY1 promoted the resistance of pepper plants to PCI with decreased disease index and transcripts of genes associated with SA biosynthesis. RNA-seq analysis showed that CaCRY1 silencing affected many genes in stress-related metabolic pathways. In summary, our findings show that CaCRY1 plays a negative role in the defense response of pepper to PCI, laying a foundation for studying the roles of CRYs in the future.

{"title":"Cryptochromes (CRYs) in pepper: Genome-wide identification, evolution and functional analysis of the negative role of CaCRY1 under Phytophthora capsici infection","authors":"Fei Sun , Yue Chen , Ying Luo , Feng Yang , Ting Yu , Huibin Han , Youxin Yang , Yong Zhou","doi":"10.1016/j.plantsci.2025.112460","DOIUrl":"10.1016/j.plantsci.2025.112460","url":null,"abstract":"<div><div>Cryptochromes (CRYs) are ultraviolet-A (UV-A) and blue light photoreceptors that perceive UV-A and blue light to mediate a range of physiological processes including disease response in plants. However, there has been no report about the roles of CRY genes in pepper, which often suffers from Phytophthora blight caused by Phytophthora capsici. In this work, three pepper CRY genes were identified and their characteristics were examined by bioinformatics analysis. CaCRY1 is an ortholog of AtCRY1 located in the cytoplasm and nucleus, and expression analysis by RT-qPCR showed that its transcription was differentially regulated by jasmonic acid (JA) and salicylic acid (SA), as well as by P. capsici infection (PCI). Overexpression of CaCRY1 in pepper and Nicotiana benthamiana promoted the susceptibility of plants to PCI. Further virus-induced gene silencing (VIGS) analysis showed that silencing of CaCRY1 promoted the resistance of pepper plants to PCI with decreased disease index and transcripts of genes associated with SA biosynthesis. RNA-seq analysis showed that CaCRY1 silencing affected many genes in stress-related metabolic pathways. In summary, our findings show that CaCRY1 plays a negative role in the defense response of pepper to PCI, laying a foundation for studying the roles of CRYs in the future.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112460"},"PeriodicalIF":4.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maize SERRATE 1B positively regulates seed germinability under low-temperature

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-03-06 DOI: 10.1016/j.plantsci.2025.112458

Qinghui Han , Zan Ren , Qingxiang Zhu , Yang Zhou , Minyi Zhu , Junguang He , Xiaomin Wang , Guangwu Zhao

Low temperature poses a significant threat to seedling emergence after maize sowing. While the impact of SERRATE (SE) on plant development via RNA processing has been extensively reported, its involvement in transcriptional regulation or the formation of low-temperature germination ability remains unclear. Our previous research revealed that ZmSE1B is located at the overlapping region of qLTGR4-1 or qLTPRL4-1, which has been associated with low-temperature germination by QTL analysis using IBM Syn4 RIL population. In the present study, we observed that maize seeds overexpressing ZmSE1B exhibited enhanced germination percentages, longer roots, and longer shoots when subjected to low-temperature conditions compared to the wildtype. Through an integrated analysis of RNA-Seq and CUT&Tag, we speculated that ZmGRXCC17, which encodes a GLUTAREDOXIN, may be upregulated by ZmSE1B in maize germinated seeds at low-temperature. Further, the regulation of ZmSE1B on transcription of ZmGRXCC17 was validated using dual-luciferase reporter system and CUT&Tag-qPCR. Finally, the positive effect of ZmGRXCC17 on low-temperature tolerance during seed germination was elucidated through its heterologous expression in rice. The results indicate that ZmSE1B enhances the seed germination ability under low temperature by regulating the transcription of ZmGRXCC17.

{"title":"Maize SERRATE 1B positively regulates seed germinability under low-temperature","authors":"Qinghui Han , Zan Ren , Qingxiang Zhu , Yang Zhou , Minyi Zhu , Junguang He , Xiaomin Wang , Guangwu Zhao","doi":"10.1016/j.plantsci.2025.112458","DOIUrl":"10.1016/j.plantsci.2025.112458","url":null,"abstract":"<div><div>Low temperature poses a significant threat to seedling emergence after maize sowing. While the impact of SERRATE (SE) on plant development via RNA processing has been extensively reported, its involvement in transcriptional regulation or the formation of low-temperature germination ability remains unclear. Our previous research revealed that ZmSE1B is located at the overlapping region of qLTGR4-1 or qLTPRL4-1, which has been associated with low-temperature germination by QTL analysis using IBM Syn4 RIL population. In the present study, we observed that maize seeds overexpressing ZmSE1B exhibited enhanced germination percentages, longer roots, and longer shoots when subjected to low-temperature conditions compared to the wildtype. Through an integrated analysis of RNA-Seq and CUT&Tag, we speculated that ZmGRXCC17, which encodes a GLUTAREDOXIN, may be upregulated by ZmSE1B in maize germinated seeds at low-temperature. Further, the regulation of ZmSE1B on transcription of ZmGRXCC17 was validated using dual-luciferase reporter system and CUT&Tag-qPCR. Finally, the positive effect of ZmGRXCC17 on low-temperature tolerance during seed germination was elucidated through its heterologous expression in rice. The results indicate that ZmSE1B enhances the seed germination ability under low temperature by regulating the transcription of ZmGRXCC17.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112458"},"PeriodicalIF":4.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allelopathic inhibitory of thymol on Arabidopsis thaliana primary root growth is mediated by ABA signaling pathway

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-03-06 DOI: 10.1016/j.plantsci.2025.112453

Liai Ma, Kai Yin, Wenhui Zhu, Yuanbo Wang, Lina Zhang, Ning Yang

Abscisic acid (ABA) is a sesquiterpenoid phytohormone involved in controlling plant root growth and development. Thymol, a monoterpene allelochemical, showed a potent phytotoxic effect in plants. It can rapidly inhibit seed germination and seedling growth. In this study, we employed a combination of transcriptome sequencing and validation methods from plant genetics and physiology to investigate the allelopathic inhibitory effects of thymol on the primary roots of Arabidopsis. We found that thymol affected the growth of Arabidopsis thaliana primary root in a dose-dependent manner, low concentration (10 μM) generally enhances, and high concentration (150 μM) inhibits. RNA sequencing analysis showed that a high concentration of thymol affected a series of biological processes and signaling transduction, including ABA biosynthesis, auxin polar transport, oxidative stress, root growth, and development. Exogenous ABA (10 μM) enhanced the inhibitory effect of thymol on the primary root and the application of the ABA biosynthesis inhibitor Na₂WO₄ rescued this inhibitory effect. During this process, the content and distribution of auxin in the roots were significantly altered. The lengths of primary root and meristem of mutant abi1, abi2, and abi1 abi2, showed that ABI1 and ABI2 positively regulate the process of thymol inhibition of root growth. In summary, the allelopathic inhibitory of thymol on Arabidopsis thaliana primary root growth is mediated by ABA signaling pathway.

{"title":"Allelopathic inhibitory of thymol on Arabidopsis thaliana primary root growth is mediated by ABA signaling pathway","authors":"Liai Ma, Kai Yin, Wenhui Zhu, Yuanbo Wang, Lina Zhang, Ning Yang","doi":"10.1016/j.plantsci.2025.112453","DOIUrl":"10.1016/j.plantsci.2025.112453","url":null,"abstract":"<div><div>Abscisic acid (ABA) is a sesquiterpenoid phytohormone involved in controlling plant root growth and development. Thymol, a monoterpene allelochemical, showed a potent phytotoxic effect in plants. It can rapidly inhibit seed germination and seedling growth. In this study, we employed a combination of transcriptome sequencing and validation methods from plant genetics and physiology to investigate the allelopathic inhibitory effects of thymol on the primary roots of Arabidopsis. We found that thymol affected the growth of Arabidopsis thaliana primary root in a dose-dependent manner, low concentration (10 μM) generally enhances, and high concentration (150 μM) inhibits. RNA sequencing analysis showed that a high concentration of thymol affected a series of biological processes and signaling transduction, including ABA biosynthesis, auxin polar transport, oxidative stress, root growth, and development. Exogenous ABA (10 μM) enhanced the inhibitory effect of thymol on the primary root and the application of the ABA biosynthesis inhibitor Na2WO4 rescued this inhibitory effect. During this process, the content and distribution of auxin in the roots were significantly altered. The lengths of primary root and meristem of mutant abi1, abi2, and abi1 abi2, showed that ABI1 and ABI2 positively regulate the process of thymol inhibition of root growth. In summary, the allelopathic inhibitory of thymol on Arabidopsis thaliana primary root growth is mediated by ABA signaling pathway.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112453"},"PeriodicalIF":4.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifaceted Role of Selenium in Plant Physiology and Stress Resilience: A Review.

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-03-04 DOI: 10.1016/j.plantsci.2025.112456

Abdullah, Kaiser Iqbal Wani, Kashif Hayat, M Naeem, Tariq Aftab

Selenium (Se) is a naturally occurring element in both seleniferous and non-seleniferous soils. Plants absorb Se in a variety of ways, mainly as selenate (SeO₄^2-), selenite (SeO₃^2-), and organic compounds such as selenomethionine (SeMet). Selenium significantly impacts plant growth, development, and stress responses. It is a trace element that regulates many physiological and biochemical functions in plants, acts as an antioxidant, and increases plant resistance to abiotic stresses such as heavy metal toxicity, salinity, drought, and severe temperatures. Its beneficial effects depend on the dose and vary depending on the plant species and the environmental conditions. Several functions of Se have been thoroughly discussed in this review, with special attention given to the mechanisms of Se uptake, transport, accumulation, and metabolism. Plants use Se through its assimilation into amino acids (mostly selenocysteine and selenomethionine) and integration into proteins. These processes might have different effects depending on the Se concentration. Furthermore, Se has the potential to be a useful tool in sustainable agriculture, especially in regions where environmental stress is common. This is demonstrated by its ability to increase plant tolerance to various environmental stressors. Recent research shows that Se supplementation not only boosts plant resistance but also enhances secondary metabolite accumulation. Overall, this review concludes that Se plays a dual role in plant systems, acting as both a nutrient and a stress mitigator, and provides opportunities to optimize its use in sustainable agriculture by tailoring Se supplementation to maximize plant tolerance and productivity.

{"title":"Multifaceted Role of Selenium in Plant Physiology and Stress Resilience: A Review.","authors":"Abdullah, Kaiser Iqbal Wani, Kashif Hayat, M Naeem, Tariq Aftab","doi":"10.1016/j.plantsci.2025.112456","DOIUrl":"https://doi.org/10.1016/j.plantsci.2025.112456","url":null,"abstract":"Selenium (Se) is a naturally occurring element in both seleniferous and non-seleniferous soils. Plants absorb Se in a variety of ways, mainly as selenate (SeO42-), selenite (SeO32-), and organic compounds such as selenomethionine (SeMet). Selenium significantly impacts plant growth, development, and stress responses. It is a trace element that regulates many physiological and biochemical functions in plants, acts as an antioxidant, and increases plant resistance to abiotic stresses such as heavy metal toxicity, salinity, drought, and severe temperatures. Its beneficial effects depend on the dose and vary depending on the plant species and the environmental conditions. Several functions of Se have been thoroughly discussed in this review, with special attention given to the mechanisms of Se uptake, transport, accumulation, and metabolism. Plants use Se through its assimilation into amino acids (mostly selenocysteine and selenomethionine) and integration into proteins. These processes might have different effects depending on the Se concentration. Furthermore, Se has the potential to be a useful tool in sustainable agriculture, especially in regions where environmental stress is common. This is demonstrated by its ability to increase plant tolerance to various environmental stressors. Recent research shows that Se supplementation not only boosts plant resistance but also enhances secondary metabolite accumulation. Overall, this review concludes that Se plays a dual role in plant systems, acting as both a nutrient and a stress mitigator, and provides opportunities to optimize its use in sustainable agriculture by tailoring Se supplementation to maximize plant tolerance and productivity.","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"112456"},"PeriodicalIF":4.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MdNAC2 enhances K+ deficiency stress tolerance by maintaining K+ homeostasis in apple

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-03-04 DOI: 10.1016/j.plantsci.2025.112455

Jianyu Li , Yaqi Cui , Tianchao Wang , Caihong Wang , Xiaodong Zheng , Zhijuan Sun , Qiang Zhao , Changqing Ma , Yi Lyu , Yike Tian

Potassium (K) is an essential nutrient for apple production, with its deficiency severely compromising yield and fruit quality. The development of K deficiency-resistant rootstocks represents an effective and promising approach to alleviating the adverse effects of K deficiency stress. However, the molecular mechanisms underlying apple resistance to K deficiency remain poorly understood. Here, we identified the transcription factor MdNAC2 as a critical regulator of apple tolerance to K deficiency through RNA-seq using Malus hupehensis as material. MdNAC2 enhanced apple tolerance to K deficiency by maintaining K⁺ homeostasis, primarily through directly suppressing the expression of the K⁺ efflux transporter MdGORK1. This regulatory mechanism reduces K⁺ efflux and stabilizes intracellular K⁺ levels under K deficiency stress. Collectively, our findings highlight the pivotal role of the MdNAC2-MdGORK1-K⁺ regulatory module in maintaining K⁺ and conferring apple resistance to K deficiency. This study provides new insights into the molecular mechanisms of K deficiency tolerance and establishes a theoretical foundation for breeding K deficiency-resistant apple rootstocks and cultivars.

钾（K）是苹果生产中不可或缺的养分，缺钾会严重影响产量和果实品质。开发抗缺钾砧木是减轻缺钾胁迫不利影响的一种有效且有前景的方法。然而，人们对苹果抗缺钾的分子机制仍然知之甚少。在这里，我们以Malus hupehensis为材料，通过RNA-seq鉴定出转录因子MdNAC2是苹果耐缺钾的关键调控因子。MdNAC2 主要通过直接抑制 K+ 外流转运体 MdGORK1 的表达，维持 K+ 的平衡，从而增强苹果对 K 缺乏的耐受性。这种调控机制减少了 K+ 外流，稳定了缺钾胁迫下的细胞内 K+ 水平。总之，我们的研究结果凸显了 MdNAC2-MdGORK1-K+ 调控模块在维持 K+ 和赋予苹果对 K 缺乏的抗性中的关键作用。这项研究为了解耐钾缺乏的分子机制提供了新的视角，并为培育耐钾缺乏的苹果砧木和栽培品种奠定了理论基础。

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引用次数: 0

The root-derived syringic acid and shoot-to-root phytohormone signaling pathways play a critical role in preventing apple scab disease

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-03-04 DOI: 10.1016/j.plantsci.2025.112457

Pratibha Demiwal , Parikshit Kumar Saini , Mukund Kumar , Partha Roy , Mahendra Kumar Verma , Javid Iqbal Mir , Debabrata Sircar

Apple scab is a serious disease that has a huge economic impact. While some cultivars of apple are scab-resistant, most are not. Growing research has suggested that root-derived metabolites play a vital role in conferring resistance to aboveground pathogens through the long-distance signaling system between shoot and root. In this work, leaves of scab-resistant cultivar ‘Prima’ (PRM) and scab-susceptible cultivar 'Red Delicious' (RD) were challenged by Venturia inaequalis, and the resulting metabolic reprogramming in root tissues was monitored using gas chromatography-mass spectrometry-based metabolomics in time-course fashion. Metabolomics has revealed that scab fungus causes metabolic reprogramming in underground root tissue when above-ground parts (leaves) are infected. After scab infection in the above-ground leaf tissue, syringic acid is synthesized in the root tissue and transported from the root to the aerial part through vascular tissue. The increased level of reactive oxygen species and jasmonic acid (JA) across roots suggests a signaling pathway from infected leaves triggered by hydrogen peroxide (H₂O₂). In this study, it was found that leaf infection with scab produces H₂O₂. In aerial parts infected with scab, H₂O₂ may act as a signaling molecule to trigger JA production. By travelling from the aerial part (shoot) to the root, H₂O₂ and JA act as long-distance signaling molecules, stimulating magnesium uptake, and eventually enhancing phenylalanine ammonia-lyase (PAL) activity. A metabolic reprogramming of the root tissue is initiated by H₂O₂, JA and PAL activity. Root metabolic reprograming results in the formation of syringic acid, which travels from the roots to the aerial part through vascular tissue and helps fight scab fungal infections. The present study demonstrated that scab infection in apple leaves is associated with long distance signaling from shoot to root, in which root-derived specialized metabolites make their way to aerial parts and confer resistance to scab.

苹果疮痂病是一种严重的病害，对经济影响巨大。虽然有些苹果栽培品种能抗疮痂病，但大多数品种却不能。越来越多的研究表明，根部衍生的代谢物通过芽和根之间的长距离信号系统在赋予地上部病原体抗性方面发挥着重要作用。在这项研究中，抗疮痂病的栽培品种 "Prima"（PRM）和易受疮痂病影响的栽培品种 "Red Delicious"（RD）的叶片受到 Venturia inaequalis 的侵袭，利用基于气相色谱-质谱联用技术的代谢组学对根部组织的代谢重编程进行了时程监测。代谢组学研究发现，疮痂病真菌感染地上部分（叶片）时，会导致地下根部组织的代谢重编程。疮痂病菌感染地上部分叶片组织后，会在根部组织中合成丁香酸，并通过维管组织从根部运输到气生部分。根部活性氧和茉莉酸（JA）水平的增加表明，过氧化氢（H2O2）是由受感染叶片引发的信号途径。本研究发现，叶片感染疮痂病会产生 H2O2。在感染疮痂病的气生部分，H2O2 可能是触发 JA 生成的信号分子。H2O2 和 JA 从气生部位（嫩枝）进入根部，成为远距离信号分子，刺激镁的吸收，最终增强苯丙氨酸氨解酶（PAL）的活性。根组织的新陈代谢重编程由 H2O2、JA 和 PAL 活性启动。根部代谢重编程导致丁香酸的形成，丁香酸通过维管组织从根部进入气生部分，有助于抵抗疮痂病真菌感染。本研究表明，苹果叶片上的疮痂病感染与从嫩枝到根的长距离信号传递有关，在这种传递过程中，根部产生的特殊代谢物会进入气生部分，并赋予气生部分对疮痂病的抗性。

{"title":"The root-derived syringic acid and shoot-to-root phytohormone signaling pathways play a critical role in preventing apple scab disease","authors":"Pratibha Demiwal , Parikshit Kumar Saini , Mukund Kumar , Partha Roy , Mahendra Kumar Verma , Javid Iqbal Mir , Debabrata Sircar","doi":"10.1016/j.plantsci.2025.112457","DOIUrl":"10.1016/j.plantsci.2025.112457","url":null,"abstract":"<div><div>Apple scab is a serious disease that has a huge economic impact. While some cultivars of apple are scab-resistant, most are not. Growing research has suggested that root-derived metabolites play a vital role in conferring resistance to aboveground pathogens through the long-distance signaling system between shoot and root. In this work, leaves of scab-resistant cultivar ‘Prima’ (PRM) and scab-susceptible cultivar 'Red Delicious' (RD) were challenged by Venturia inaequalis, and the resulting metabolic reprogramming in root tissues was monitored using gas chromatography-mass spectrometry-based metabolomics in time-course fashion. Metabolomics has revealed that scab fungus causes metabolic reprogramming in underground root tissue when above-ground parts (leaves) are infected. After scab infection in the above-ground leaf tissue, syringic acid is synthesized in the root tissue and transported from the root to the aerial part through vascular tissue. The increased level of reactive oxygen species and jasmonic acid (JA) across roots suggests a signaling pathway from infected leaves triggered by hydrogen peroxide (H2O2). In this study, it was found that leaf infection with scab produces H2O2. In aerial parts infected with scab, H2O2 may act as a signaling molecule to trigger JA production. By travelling from the aerial part (shoot) to the root, H2O2 and JA act as long-distance signaling molecules, stimulating magnesium uptake, and eventually enhancing phenylalanine ammonia-lyase (PAL) activity. A metabolic reprogramming of the root tissue is initiated by H2O2, JA and PAL activity. Root metabolic reprograming results in the formation of syringic acid, which travels from the roots to the aerial part through vascular tissue and helps fight scab fungal infections. The present study demonstrated that scab infection in apple leaves is associated with long distance signaling from shoot to root, in which root-derived specialized metabolites make their way to aerial parts and confer resistance to scab.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112457"},"PeriodicalIF":4.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0