Plant Science最新文献_第2页

Functional analysis of FveBZR1-2 reveals a potential auxin–BR regulatory module in early receptacle elongation of strawberry FveBZR1-2的功能分析揭示了草莓早期花托伸长中潜在的生长素- br调控模块。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-05-01 Epub Date: 2026-02-23 DOI: 10.1016/j.plantsci.2026.113072

Tingting Han , Yongqi Zhang , Yajun Tang , Xiaodan Zhao , Guoming Wang , Junhui Zhou

Brassinazole-resistant 1 (BZR1) transcription factors are central regulators of brassinosteroid (BR) signaling and play key roles in plant growth and development, often through crosstalk with other hormone pathways such as auxin. However, their functions in strawberry remain largely unexplored. In this study, we performed a genome-wide identification and characterization of BZR1 family genes in woodland strawberry (Fragaria vesca). Seven FveBZR1 genes encoding 11 transcript isoforms were identified and cloned. All the FveBZR1 family genes systematically analyzed with respect to their physicochemical properties, phylogeny, conserved motifs, gene structures, and promoter cis-elements. Expression profiling revealed divergent spatiotemporal patterns, among which FveBZR1–2 was predominantly expressed in early receptacle stages, suggesting a role in fruit development. Functional validation demonstrated that overexpression of FveBZR1–2 promoted receptacle elongation at early floral stages, whereas no significant effect was observed at later stages. Furthermore, LCI assays revealed that FveARF6 associates with multiple FveBZR1 proteins, including FveBZR1–2. The interaction between FveARF6 and FveBZR1–2 was further supported by Y2H and BiFC assays, while Co-IP assays provided additional biochemical evidence for the association between FveARF6 and another FveBZR1 member. Together, these findings provide the first comprehensive insight into the FveBZR1 gene family in strawberry and highlight FveBZR1–2 as a potential regulator of early receptacle elongation, advancing our understanding of hormone-mediated control of strawberry fruit development.

brassinazol -resistant 1 （BZR1）转录因子是油菜素内酯（brassinosteroids， BR）信号传导的中心调控因子，通常通过与生长素等其他激素通路的串扰，在植物生长发育中发挥关键作用。然而，它们在草莓中的作用在很大程度上仍未被探索。在本研究中，我们对林地草莓（Fragaria vesca） BZR1家族基因进行了全基因组鉴定和鉴定。鉴定并克隆了7个FveBZR1基因，共编码11个转录物亚型。系统分析了FveBZR1家族所有基因的理化性质、系统发育、保守基序、基因结构和启动子顺式元件。FveBZR1-2主要在花托早期表达，表明其在果实发育中起一定作用。功能验证表明，过表达FveBZR1-2在花期早期促进花托伸长，而在花期后期无显著影响。此外，LCI分析显示，FveARF6与多个FveBZR1蛋白结合，包括FveBZR1-2。Y2H和BiFC实验进一步证实了FveARF6与FveBZR1-2之间的相互作用，而Co-IP实验为FveARF6与另一个FveBZR1成员之间的关联提供了额外的生化证据。总之，这些发现首次全面了解了草莓中FveBZR1基因家族，并强调了FveBZR1-2作为早期花托伸长的潜在调节因子，促进了我们对激素介导的草莓果实发育控制的理解。

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引用次数: 0

RlTTG1 isolated from Rosa laevigata Michx. regulates trichome development and stress response in transgenic Arabidopsis 从月季中分离得到RlTTG1。调控转基因拟南芥毛状体发育和胁迫响应。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-05-01 Epub Date: 2026-02-26 DOI: 10.1016/j.plantsci.2026.113080

Sinian Wu , Lisha Song , Yixi Chen , Cong Luo , Lingyun Wan , Guojian Liao , Wenjing Liang , Shugen Wei , Cuihong Yang , Yujian Huang , Ying Wei , Shixin Feng , Jine Fu , Haixia Yu

Rosa laevigata Michx. (R. laevigata) is an important horticultural plant with strong drought resistance and ornamental value. Its fruit can be used as medicine, but its fruits and stems are covered with dense prickles, which leads to difficulty for cultivation. The molecular mechanism of prickle development in R. laevigata is still unknown. In the present study, eighty-one prickle-related genes were found by transcriptome sequencing. A gene closely related to prickle development was selected for functional research, which was named TRANSPARENT TESTA GLABRA 1 (TTG1). RlTTG1 (Rl corresponds to R. laevigata) is highly expressed in the prickles rich tissues. Overexpression of RlTTG1 in Arabidopsis (At represents the species of Arabidopsis), a model plant where epidermal protrusions are termed trichomes (unlike the prickles in R. laevigata), caused an increased number of leaf trichomes but reduced root hair growth. When exposed to abiotic stress, the roots of overexpressed RlTTG1 lines grew longer than the wild type. And increased the seed germination rate under drought and salt stress. Physiological and biochemical tests showed that the contents of proline (Pro) and superoxide dismutase (SOD) activity were increased, while the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2) were significantly reduced. In addition, Yeast two-hybrid (Y2H) and Bimolecular fluorescence complementation (BiFC) experiments found that RlTTG1 can interact with several trichome-related proteins, including ENHANCER OF TRY AND CPC 1 (RlETC1), ENHANCER OF TRY AND CPC 3 (RlETC3), Glabra 1a (RlGL1a), Glabra 1b (RlGL1b), and ENHANCER OF GLABRA 3 (RlEGL3). Overall, this study lays the foundation for the research on the molecular mechanism of prickle development and stress response in R. laevigata.

玫蔷薇。是一种具有较强抗旱性和观赏价值的重要园艺植物。它的果实可以作为药物使用，但它的果实和茎上覆盖着密集的刺，这导致种植困难。刺虫发育的分子机制尚不清楚。在本研究中，通过转录组测序发现了81个刺相关基因。选择一个与刺发育密切相关的基因进行功能研究，命名为TRANSPARENT TESTA GLABRA 1 （TTG1）。RlTTG1 （Rl对应于r.l laevigata）在多刺组织中高表达。在拟南芥（At表示拟南芥）中，RlTTG1的过表达导致叶片毛状体数量增加，而根毛生长减少。拟南芥是一种表皮突起被称为毛状体的模式植物（与R. laevigata中的皮刺不同）。当暴露于非生物胁迫时，过表达RlTTG1系的根比野生型长。在干旱和盐胁迫下提高种子发芽率。生理生化试验表明，脯氨酸（Pro）含量和超氧化物歧化酶（SOD）活性显著升高，丙二醛（MDA）和过氧化氢（H2O2）含量显著降低。此外，酵母双杂交（Y2H）和双分子荧光互补（BiFC）实验发现，RlTTG1可以与几种毛状体相关蛋白相互作用，包括TRY和CPC 1的ENHANCER （RlETC1）、TRY和CPC 3的ENHANCER （RlETC3）、Glabra 1a （RlGL1a）、Glabra 1b （RlGL1b）和Glabra 3的ENHANCER （RlEGL3）。本研究为深入研究刺刺发育和胁迫响应的分子机制奠定了基础。

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引用次数: 0

Overexpression of the Mentha canadensis McTTG2 gene positively regulates trichome development and proanthocyanidins biosynthesis in transgenic Arabidopsis 加拿大薄荷McTTG2基因的过表达正调控转基因拟南芥的毛状体发育和原花青素的生物合成。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-05-01 Epub Date: 2026-02-19 DOI: 10.1016/j.plantsci.2026.113065

Yang Bai , Yichuan Xu , Xiwu Qi , Li Li , Xu Yu , Hailing Fang , Dongmei Liu , Fei Chen , Chengyuan Liang

WRKY transcription factors play key roles in plant development and growth, as well as in response to stress and metabolism. Hence, WRKY members have been identified in many plant species; however, few reports are available on the WRKY family in mint (Mentha canadensis). Therefore, this study employed phylogenetic analyses to identify a mint WRKY Group I gene named McTTG2. McTTG2 is widely expressed in both vegetative and reproductive organs, encoding a nuclear protein with no transcriptional auto-activation activity in yeast cells. By a heterologous complementation study, McTTG2 was overexpressed in Arabidopsis thaliana ttg2-7 mutant plants, which significantly increased trichome number and proanthocyanidin (PA) accumulation. Consistent with these phenotypes in McTTG2-overexpressing lines, the expression of several key genes involved in trichome formation, such as AtGL2, AtTRY, and AtBRK1, as well as PA biosynthesis, including AtTT12 and AtTT13, was altered. Y1H and Dual-Luciferase assays revealed that McTTG2 could bind the promoters and activate the expression of these genes. These results suggest that McTTG2 positively controls trichome development and PA biosynthesis by regulating the expression of genes related to trichome development (AtGL2, AtTRY, and AtBRK1) and PA biosynthesis (AtTT12 and AtTT13) in transgenic plants.

WRKY转录因子在植物发育、生长、逆境响应和代谢等方面发挥着重要作用。因此，WRKY成员已在许多植物物种中被发现；然而，关于薄荷（加拿大薄荷）中WRKY家族的报道很少。因此，本研究通过系统发育分析鉴定了薄荷WRKY I组基因McTTG2。McTTG2在酵母细胞中广泛表达于营养器官和生殖器官，编码一种无转录自激活活性的核蛋白。通过异源互补研究，McTTG2在拟南芥ttg2-7突变体中过表达，显著增加毛状体数量和原花青素（PA）积累。与mcttg2过表达系的表型一致，参与毛状体形成的几个关键基因（如AtGL2、AtTRY和AtBRK1）以及PA生物合成的几个关键基因（包括AtTT12和AtTT13）的表达发生了改变。Y1H和Dual-Luciferase实验显示McTTG2可以结合启动子并激活这些基因的表达。上述结果表明，McTTG2通过调控转基因植物毛状体发育相关基因（AtGL2、AtTRY和AtBRK1）和PA生物合成相关基因（AtTT12和AtTT13）的表达，积极调控毛状体发育和PA生物合成。

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引用次数: 0

Overexpression of PbWRKY66 negatively regulate phosphate uptake and root-to-shoot distribution in Arabidopsis PbWRKY66的过表达对拟南芥的磷酸盐吸收和根到茎的分布有负调控作用。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-05-01 Epub Date: 2026-02-20 DOI: 10.1016/j.plantsci.2026.113069

Mengdan Li , Wenyu Sun , Mengjie Wu, Fangyan Li, Zhen Cao, Nian Liu, Longjun Cheng

Phoebe bournei is a valuable timber species endemic to southern China, renowned for its economic and ecological importance. However, the widespread low phosphorus level in the acidic soils of this region severely constrains the productivity of P. bournei plantations. Elucidating the molecular mechanisms governing phosphorus uptake, translocation, and homeostasis in P. bournei is therefore essential for breeding varieties with enhanced tolerance to low phosphorus conditions. The current study focuses on PbWRKY66, a group II WRKY transcription factor that was identified and characterized in Phoebe bournei. Under phosphorus deficient conditions, PbWRKY66 expression was markedly induced in leaves but strongly suppressed in roots during prolonged phosphorus deprivation. The PbWRKY66 protein was localized in the nucleus and exhibited transactivation activity. Expression analysis in different tissues revealed that PbWRKY66 transcripts accumulated predominantly in stems and leaves, while the assays of GUS reporter driven by promoter of PbWRKY66 demonstrated strong promoter activity in leaves vein, vascular tissues of stems and roots, flower stalk base, and lateral root tips. Transgenic Arabidopsis thaliana lines overexpressing PbWRKY66 displayed higher sensitivity to phosphorus deficiency, characterized by reduced root elongation, small aerial parts, and increased anthocyanin accumulation compared to the wild type. Phosphorus starvation also resulted in markedly lower total phosphorus in shoots and roots and reduced inorganic phosphate in leaves of the transgenic lines compared with those of the wild type plants. Moreover, the expression of AtPHT1;1, AtPHT1;4, and AtPHO1, which are central to phosphorus uptake and transport, was substantially repressed in PbWRKY66-OE roots, whereas AtSPX1, a negative regulator of the phosphate starvation response, was upregulated. Collectively, these findings point to PbWRKY66 functioning as a negative modulator in the control of phosphorus uptake, translocation from roots to shoots, and phosphate starvation signaling, thereby contributing to the modulation of phosphorus homeostasis under phosphorus deficiency stress in plants.

菲比是中国南方特有的珍贵木材树种，因其经济和生态重要性而闻名。然而，该地区酸性土壤普遍存在低磷现象，严重制约了柏树人工林的生产力。因此，阐明伯氏拟南芥对磷的吸收、转运和体内平衡的分子机制，对于培育对低磷条件具有更高耐受性的品种至关重要。目前的研究重点是PbWRKY66，这是一种II组WRKY转录因子，在Phoebe bournei中被鉴定和表征。缺磷条件下，PbWRKY66的表达在叶片中被显著诱导，而在根系中被强烈抑制。PbWRKY66蛋白定位于细胞核，并表现出反活化活性。在不同组织中的表达分析显示，PbWRKY66转录本主要在茎和叶中积累，而PbWRKY66启动子驱动的GUS报告子在叶脉、茎和根的维管组织、花茎基部和侧根尖中都有较强的启动子活性。与野生型相比，过表达PbWRKY66的转基因拟南芥对缺磷的敏感性更高，根系伸长减少，地上部分较小，花青素积累增加。与野生型植物相比，磷饥饿还导致转基因品系的茎、根总磷显著降低，叶片无机磷显著减少。此外，AtPHT1；1、AtPHT1;在PbWRKY66-OE根中，对磷的吸收和运输起核心作用的AtPHO1被显著抑制，而磷酸盐饥饿反应的负调节因子AtSPX1则被上调。总的来说，这些发现表明PbWRKY66在控制磷吸收、从根到芽的转运和磷酸盐饥饿信号中起负调节作用，从而有助于调节植物在缺磷胁迫下的磷稳态。

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引用次数: 0

Endosperm-specific overexpression of Opaque2 improves maize kernel texture 胚乳特异性过表达不透明蛋白2可改善玉米籽粒结构。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-05-01 Epub Date: 2026-02-28 DOI: 10.1016/j.plantsci.2026.113085

Zan Wu, Tao Yang

Kernel texture is a crucial agronomic trait that determines crop yield and nutrient quality, yet limited genes with breeding potentials have been identified for texture improvement. In maize, the endosperm-filling central regulator Opaque2 coordinately regulates starch and protein synthesis and plays pivotal roles in vitreous endosperm formation. In this study, we specifically overexpressed Opaque2 in the developing endosperm driven by the 27-kD γ zein promoter to investigate the effects on kernel texture, yield and nutrient quality. Notably, overexpression of Opaque2 enhanced test weight and 100-kernel weight despite reduced kernel dimensions. The overexpressed kernels exhibited improved kernel texture with expanded vitreous endosperm regions. Furthermore, the starch contents were increased, including elevated levels of amylose and amylopectin. The starch granules in the overexpressed endosperms were denser and more tightly packed. Zein accumulation was dramatically elevated in the overexpressed kernels in comparison with wild type, whereas nonzein levels were slightly decreased, resulting in elevated total protein synthesis. Further transcriptomic analysis comprehensively revealed that the Opaque2 overexpression positively influences a series of biological processes to precisely modulate vitreous endosperm formation and texture improvement. This work provides a novel insight into maize texture improvement through endosperm-specific overexpression of Opaque2.

籽粒质地是决定作物产量和营养品质的重要农艺性状，但目前发现的具有改良籽粒质地潜力的基因有限。在玉米中，胚乳填充中心调控蛋白不透明蛋白2协调调节淀粉和蛋白质的合成，并在玻璃体胚乳形成中起关键作用。在本研究中，我们在27-kD γ玉米蛋白启动子驱动的发育胚乳中特异性过表达不透明蛋白2，以研究其对籽粒质地、产量和营养品质的影响。值得注意的是，尽管降低了内核尺寸，但过表达Opaque2增强了测试权值和100核权值。过表达的籽粒结构得到改善，玻璃状胚乳区域扩大。此外，淀粉含量增加，包括直链淀粉和支链淀粉水平升高。过表达胚乳中淀粉颗粒密度更大，排列更紧密。与野生型相比，玉米蛋白积累显著增加，而非玉米蛋白水平略有下降，导致总蛋白合成升高。进一步的转录组学分析全面揭示了Opaque2过表达对一系列生物学过程产生积极影响，从而精确调节玻璃体胚乳的形成和质地改善。这项工作为通过胚乳特异性过表达Opaque2改善玉米质地提供了新的见解。

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引用次数: 0

Seed priming with silicon nanoparticles and nitric oxide optimizes barley growth in zinc-deficient condition: A crucial role of optimum level of endogenous nitric oxide 在缺锌条件下，用硅纳米颗粒和一氧化氮灌种可以优化大麦的生长：内源性一氧化氮的最佳水平起着至关重要的作用。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-05-01 Epub Date: 2026-01-31 DOI: 10.1016/j.plantsci.2026.112998

Nidhi Kandhol , Sangeeta Pandey , Santosh Kumar , Shivesh Sharma , Samiksha Singh , Prasanta K. Dash , Durgesh Kumar Tripathi

Zinc (Zn) deficiency significantly impacts plant growth and productivity in agriculture. Seed priming is a promising strategy to enhance plant tolerance to nutrient deficiencies. This study examines the effects of priming barley (Hordeum vulgare L.) seeds with silicon nanoparticles (SiNPs), nitric oxide (NO), and their combination on germination and growth under Zn-deficient conditions. Primed seedlings showed superior growth, and improved photosynthetic efficiency, antioxidant enzyme activities, the ascorbate-glutathione cycle function, nutrient-related gene expression, and sucrose metabolism compared to the un-primed seedlings. Among the priming methods, the combination of SiNPs and NO had the most significant positive effect on barley growth under Zn deficiency. Priming of seeds with SiNPs was more effective against Zn deficiency than external SiNPs application at the seedling stage. Exogenous SiNPs added to already SiNPs-primed seedlings further improved growth under Zn deficiency. Contrary to this, NO addition to NO-primed seedlings inhibited growth due to excessive endogenous NO accumulation. Co-application of SiNPs and NO to SiNPs+NO- primed seedlings led to severe growth retardation due to build-up of endogenous NO production. These findings highlight seed priming's potential, especially with SiNPs, to address nutrient deficiencies in agriculture and the complex interactions of endogenous NO in priming-mediated regulation of Zn deficiency in barley.

锌（Zn）缺乏严重影响农业植物的生长和生产力。种子启动是提高植物对营养缺乏的耐受性的一种很有前途的策略。在缺锌条件下，研究了纳米硅（SiNPs）和一氧化氮（NO）及其组合对大麦种子萌发和生长的影响。与未处理的幼苗相比，处理后的幼苗表现出更强的生长能力，光合效率、抗氧化酶活性、抗坏血酸-谷胱甘肽循环功能、营养相关基因表达和蔗糖代谢均有所提高。其中，SiNPs与NO组合对缺锌条件下大麦生长的正向影响最为显著。单独启动SiNPs比外用SiNPs更有效。在缺锌条件下，添加外源SiNPs进一步促进了幼苗的生长。与此相反，添加NO的幼苗由于内源NO积累过多而抑制生长。SiNPs和NO共同施用于SiNPs+NO引发的幼苗，由于内源性NO产量的增加，导致严重的生长迟缓。这些发现强调了种子启动的潜力，特别是与SiNPs一起，解决农业营养缺乏问题，以及内源NO在启动介导的大麦锌缺乏症调节中的复杂相互作用。

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引用次数: 0

Genome-wide characterization and functional analysis of ScALDH genes reveals their contribution to growth maintenance and drought tolerance in plants ScALDH基因的全基因组特征和功能分析揭示了它们在植物生长维持和抗旱方面的作用。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-05-01 Epub Date: 2026-02-18 DOI: 10.1016/j.plantsci.2026.113040

Haojie Fan , Aerguli Jiamahate , Jianwei Zhang , Honglan Yang , Tohir A. Bozorov , Xiaoshuan Li , Xiujin Liu , Jinbiao Ma , Jianbo Zhu , Daoyuan Zhang

Plants are often subjected to drought stress, which can significantly inhibit their growth and development. Previous studies have found that the aldehyde dehydrogenase (ALDH) gene family plays an important role in plant stress adaptation by detoxifying reactive aldehydes and mitigating oxidative damage. In this study, 18 ALDH genes were identified and systematically analyzed from the telomere-to-telomere (T2T) genome of the desiccation-tolerant Syntrichia caninervis, an excellent tolerance moss to drought, cold and radiation. Phylogenetic analysis classified these ScALDHs into 11 subfamilies, demonstrating high conservation across plant lineages from bryophytes to angiosperms. Promoter region analysis revealed an abundance of stress-responsive cis-acting elements with ABRE and ARE motifs. Transcriptomic profiling demonstrated significant upregulation of key ScALDH genes under dehydration, rehydration, and ABA treatments, including ScALDH2B2, ScALDH7B4, ScALDH11A2, and ScALDH21A2. Weighted Gene Co-expression Network Analysis (WGCNA) further implicated these genes are involved in pathways related to drought response, oxidoreductase activity, and photosynthesis regulation. Heterologous expression of these canditate genes in Arabidopsis thaliana enhanced drought tolerance by improved root architecture, elevated ROS scavenging capacity. The study reveals the evolutionary conservation and functional diversity of ALDH genes in S.caninervis, providing a theoretical foundation for their application in stress-resistant crop breeding.

植物经常遭受干旱胁迫，干旱胁迫会显著抑制植物的生长发育。已有研究发现，醛脱氢酶（ALDH）基因家族通过解毒活性醛和减轻氧化损伤在植物逆境适应中起重要作用。本研究从耐旱、耐寒、耐辐射苔藓犬齿藓（Syntrichia caninervis）的端粒到端粒（T2T）基因组中鉴定了18个ALDH基因，并对其进行了系统分析。系统发育分析将这些ScALDHs分为11个亚科，显示出从苔藓植物到被子植物的高度保守性。启动子区分析显示大量具有ABRE和ARE基序的应激响应顺式作用元件。转录组学分析显示，在脱水、补液和ABA处理下，ScALDH2B2、ScALDH7B4、ScALDH11A2和ScALDH21A2等关键ScALDH基因显著上调。加权基因共表达网络分析（WGCNA）进一步表明，这些基因参与了干旱响应、氧化还原酶活性和光合作用调控的相关途径。这些候选基因在拟南芥中的异源表达通过改善根结构和提高活性氧清除能力来增强耐旱性。该研究揭示了caninervis ALDH基因的进化保守性和功能多样性，为其在作物抗逆性育种中的应用提供了理论基础。

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引用次数: 0

MaMYB102 enhances salt tolerance in banana (Musa acuminata) by activating MaBADH-mediated glycine betaine biosynthesis MaMYB102通过激活mabadh介导的甘氨酸甜菜碱生物合成增强香蕉（Musa acuminata）的耐盐性。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-05-01 Epub Date: 2026-02-26 DOI: 10.1016/j.plantsci.2026.113081

Jiaxuan Yu , Jing He , Bowei Zhu , Muhammad Moaaz Ali , Juhua Liu , Xinguo Li

Salinity is a major abiotic stress that limits banana (Musa acuminata) growth and productivity. Transcription factors play crucial roles in regulating plant stress responses; however, the molecular mechanisms underlying salt tolerance in banana remain largely unexplored. In this study, we identified and functionally characterized MaMYB102, an R2R3-type MYB transcription factor that positively regulates salt tolerance in banana. Subcellular localization analysis confirmed that MaMYB102 is a nuclear protein. Transient overexpression of MaMYB102 in banana leaves enhanced salt tolerance, as evidenced by reduced chlorosis, elevated antioxidant capacity, and decreased hydrogen peroxide (H₂O₂) accumulation under 0.3 M NaCl treatment. Conversely, transient silencing of MaMYB102 increased salt sensitivity and oxidative damage. Yeast one-hybrid (Y1H) and electrophoretic mobility shift assays (EMSA) demonstrated that MaMYB102 binds directly to the promoter region of MaBADH, a key gene involved in glycine betaine (GB) biosynthesis. Dual-luciferase assays further confirmed that MaMYB102 activates MaBADH transcription. Overexpression of MaMYB102 increased MaBADH expression and GB accumulation, whereas silencing MaMYB102 suppressed both. Collectively, our results reveal that MaMYB102 enhances salt tolerance in banana by activating MaBADH-mediated glycine betaine biosynthesis, thereby improving redox homeostasis under saline conditions. This study provides novel insights into the MYB-regulated salt stress response and offers a potential target for developing salt-tolerant banana cultivars.

盐度是限制香蕉（Musa acuminata）生长和产量的主要非生物胁迫。转录因子在调控植物逆境反应中发挥重要作用；然而，香蕉耐盐性的分子机制在很大程度上仍未被探索。在这项研究中，我们鉴定并功能表征了一个正调控香蕉耐盐性的r2r3型MYB转录因子MaMYB102。亚细胞定位分析证实MaMYB102是一个核蛋白。在0.3M NaCl处理下，香蕉叶片短暂过表达MaMYB102增强了耐盐性，表现为黄化程度降低、抗氧化能力增强、过氧化氢积累减少。相反，短暂的沉默会增加MaMYB102的盐敏感性和氧化损伤。酵母单杂交（Y1H）和电泳迁移转移（EMSA）实验表明，MaMYB102直接结合到参与甘氨酸甜菜碱（GB）生物合成的关键基因MaBADH的启动子区域。双荧光素酶实验进一步证实，MaMYB102激活MaBADH转录。过表达MaMYB102增加了MaBADH的表达和GB的积累，而沉默MaMYB102则抑制了两者的表达。总之，我们的研究结果表明，MaMYB102通过激活mabadh介导的甘氨酸甜菜碱生物合成来增强香蕉的耐盐性，从而改善盐条件下的氧化还原稳态。该研究为研究myb调控的盐胁迫反应提供了新的见解，并为培育耐盐香蕉品种提供了潜在的靶点。

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引用次数: 0

VvTPS14 is a linalool synthase activated by VvbZIP3 and repressed by VvMADS4 VvTPS14是一种由VvbZIP3激活、VvMADS4抑制的芳樟醇合成酶。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-04-01 Epub Date: 2026-02-09 DOI: 10.1016/j.plantsci.2026.113048

Ziye Nie , Xiaochen Wang , Xianju Liu , Yi Wang , Tingting Zhang , Zhenchang Liang , Peige Fan

Linalool is a key aromatic terpene that imparts the characteristic Muscat flavor in grape berries. However, the upstream transcriptional mechanisms regulating its biosynthesis remain largely unknown. Here, integrated GC-MS and transcriptomic analyses across berry development in Muscat and non-Muscat cultivars identified VvTPS14 as a terpene synthase gene whose expression strongly correlated with the linalool accumulation pattern. Functional characterization confirmed that VvTPS14–2 encodes a chloroplast-localized enzyme that catalyzes linalool biosynthesis from GPP in vitro. Transient and stable overexpression of VvTPS14–2 in Nicotiana benthamiana leaves, grape berries, and Vitis amurensis callus consistently enhanced linalool production, establishing VvTPS14 as a key linalool synthase. Upstream regulatory mechanisms of VvTPS14 were elucidated through yeast one-hybrid screening, which identified VvbZIP3 and VvMADS4 as direct binders of the VvTPS14 promoter. Dual-luciferase and overexpression assays demonstrated that VvbZIP3–4 (a splice variant) activates, whereas VvMADS4 represses, the expression of VvTPS14. This regulatory pattern was conserved across heterologous and homologous systems. These findings reveal a complete transcriptional regulatory module controlling linalool biosynthesis and provide potential targets for molecular breeding of flavor traits in grapes.

芳樟醇是一种重要的芳香萜，赋予葡萄浆果特有的马斯喀特风味。然而，调控其生物合成的上游转录机制在很大程度上仍然未知。通过对马斯喀特和非马斯喀特品种浆果发育的综合GC-MS和转录组学分析，发现VvTPS14是一个萜烯合成酶基因，其表达与芳樟醇积累模式密切相关。功能鉴定证实，VvTPS14-2编码叶绿体定位酶，催化GPP体外生物合成芳樟醇。VvTPS14-2在烟叶、葡萄果实和葡萄愈伤组织中的瞬时和稳定过表达，持续提高了芳樟醇的产量，证实了VvTPS14是关键的芳樟醇合成酶。通过酵母单杂交筛选，明确了VvTPS14的上游调控机制，发现VvbZIP3和VvMADS4是VvTPS14启动子的直接结合物。双荧光素酶和过表达实验表明，VvbZIP3-4（一个剪接变体）激活了VvTPS14的表达，而VvMADS4抑制了VvTPS14的表达。这种调控模式在异源和同源系统中都是保守的。这些发现揭示了控制芳樟醇生物合成的完整转录调控模块，为葡萄风味性状的分子育种提供了潜在靶点。

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引用次数: 0

Genome-wide characterization of the TCP gene family in red clover (Trifolium pratense) and functional analysis of TpTCP18 in branching regulation 红三叶草TCP基因家族的全基因组特征及TpTCP18在分支调控中的功能分析

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-04-01 Epub Date: 2026-02-12 DOI: 10.1016/j.plantsci.2026.113051

Dandan Su , Wenhua Du

The TCP (Teosinte Branched1/Cycloidea/Proliferating Cell Factor) gene family encodes plant-specific transcription factors that play essential roles in regulating plant architecture, hormone signaling, and stress responses. While TCP genes have been characterized in model species, their genome-wide identification and functional roles in red clover (Trifolium pratense), a globally significant perennial forage legume, remain largely unexplored. In this study, 25 TpTCP genes were identified in the red clover genome and classified into three subfamilies: PCF, CYC/TB1, and CIN. Comparative genomic analysis suggests that the expansion of the TpTCP family was primarily driven by polyploidy levels and specific duplication events, rather than genome size. Members within each clade exhibited consistent exon-intron organization and conserved motif compositions. Structural analysis confirmed that these proteins possess a characteristic basic-helix I-loop-helix II domain. Promoter analysis revealed abundant cis-elements related to growth, hormonal regulation, and abiotic stress. Integrating phylogenetic analysis with transcriptome profiling, we identified TpTCP18, a member of the CYC/TB1 subclade, as a primary candidate due to its high tissue-specific expression in axillary buds and lateral branches (up to 34-fold higher than in roots). Subcellular localization confirmed that TpTCP18 is a nuclear-localized protein. Heterologous overexpression of TpTCP18 in Arabidopsis thaliana significantly reduced shoot branching, delayed bolting, and modified leaf morphology. Molecular characterization revealed that TpTCP18 integrates into the endogenous regulatory network of A. thaliana by significantly up-regulating the transcript levels of the branching repressor AtHB21 in lateral organs. This study provides the first comprehensive analysis of the TCP gene family in red clover and identifies TpTCP18 as a conserved negative regulator of axillary bud outgrowth. These findings demonstrate that TpTCP18 modulates plant architecture through the TCP-HB signaling module, offering a valuable molecular target for the genetic improvement of forage yield and quality in red clover.

TCP （Teosinte Branched1/Cycloidea/ proliferation Cell Factor）基因家族编码植物特异性转录因子，在调节植物结构、激素信号和逆境反应中发挥重要作用。虽然TCP基因已经在模式物种中被表征，但它们在红三叶草（Trifolium pratense）中的全基因组鉴定和功能作用仍未得到充分研究。红三叶草是一种全球重要的多年生饲草豆科植物。本研究在红三叶草基因组中鉴定出25个TpTCP基因，并将其划分为PCF、CYC/TB1和CIN三个亚家族。比较基因组分析表明，TpTCP家族的扩展主要是由多倍体水平和特定的重复事件驱动的，而不是基因组大小。每个分支的成员都表现出一致的外显子-内含子组织和保守的基序组成。结构分析证实这些蛋白具有典型的碱基-螺旋i环-螺旋II结构域。启动子分析揭示了大量与生长、激素调节和非生物胁迫相关的顺式元件。结合系统发育分析和转录组分析，我们确定了CYC/TB1亚枝的成员TpTCP18作为主要候选者，因为它在腋芽和侧枝中的组织特异性表达高（比根高34倍）。亚细胞定位证实TpTCP18是一个核定位蛋白。TpTCP18在拟南芥中异源过表达可显著减少芽分枝、延迟抽苔和改变叶片形态。分子表征表明，TpTCP18通过显著上调侧壁器官分支抑制因子AtHB21的转录水平，整合到拟南芥的内源性调控网络中。本研究首次对红三叶草中TCP基因家族进行了全面分析，并鉴定出TpTCP18是一个保守的腋芽生长负调控因子。这些发现表明，TpTCP18通过TCP-HB信号模块调控植物结构，为红三叶草产量和品质的遗传改良提供了有价值的分子靶点。

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引用次数: 0