Plant Science最新文献_第2页

Tolerance of Oryza sativa to low phosphate is associated with adaptive changes in root architecture and metabolic exudates

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-02-03 DOI: 10.1016/j.plantsci.2025.112415

Akanksha Srivastava , Amber Gupta , Sujit K. Bishi , Pole Akhila , P.C. Latha , D. Subrahmanyam , P. Brajendra , M.S. Anantha , Suvarna Rani Ch , Akshay S. Sakhare , Vijai Pal Bhadana , Jitender Giri , C.N. Neeraja , R.M. Sundaram , Satendra K. Mangrauthia

The optimum usage of fertilizers is key for the sustainable agriculture. Among nutrients, phosphorus (P) is critical for plant growth and development. Due to complete reliance on natural resources (rock phosphate) for P, the availability of P fertilizers is emerging as a global challenge for crop cultivation. Moreover, the excess application of P fertilizers in rice, mostly grown under flooded conditions, leads to water pollution called eutrophication. In this study, we employed a mutagenesis approach for developing and characterizing rice EMS (ethyl methanesulfonate) mutants with better adaptation to low soil P conditions. One such mutant of rice cultivar Nagina 22, named NH4824, was characterized comprehensively at seedling and reproductive growth stages under hydroponic and field conditions. The mutant exhibits low soil P tolerance due to combined adaptive changes in root system architecture, anatomy, organic acid exudates, plasma membrane (PM) H⁺-ATPase activity, induced expression of P transporter genes, and efficient mobilization and partitioning of P in different plant tissues. The activity of antioxidant enzymes and better photosynthesis suggested relatively less stress experienced by NH4824 than N22 under low soil P conditions. These insights are highly useful to develop P use efficient crop cultivars through breeding or genome editing approaches.

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引用次数: 0

A critical review of the importance of Far-Related Sequence (FRS)- FRS-Related Factor (FRF) transcription factors in plants

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-02-01 DOI: 10.1016/j.plantsci.2025.112410

Fereshteh Jafari , Aria Dolatabadian

Transposable elements have long been recognised as critical drivers of genetic diversity and evolution in plant genomes, influencing various physiological and developmental processes. The transcription factor family FAR-RED ELONGATED HYPOCOTYLS3 (FHY3), and its homologue FAR-RED IMPAIRED RESPONSE1 (FAR1), initially identified as key components of phytochrome A (phyA)-mediated far-red (FR) light signalling in Arabidopsis thaliana, are derived from transposases and are essential for light signal transduction, plant growth, and development. FHY3 and FAR1 are also the founding members of the FAR1-RELATED SEQUENCE (FRS) family, which is conserved across terrestrial plants. While the coding sequences of many putative FRS and FAR1-RELATED FACTOR (FRF) orthologs have been identified in various angiosperm clades, their physiological functions remain largely unexplored. The FRF genes are considered truncated forms of FRS proteins that compete with FRS for DNA binding sites, thereby regulating gene expression.

This review highlights recent advances in characterising the molecular mechanisms of FHY3, FAR1, and other members of the FRS-FRF protein family. We examine their roles in key processes such as regulating flowering time, controlling branching, integrating leaf aging and senescence, modulating the circadian clock, maintaining meristem function, starch synthesis, seed germination, and responding to Starch synthesis and carbon starvation. Additionally, we explore their contributions to plant immunity under biotic and abiotic stresses. Finally, we suggest future directions for functional characterising other FRS-FRF family proteins in plants, which could provide deeper insights into their regulatory roles in plant biology.

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引用次数: 0

Sugar and anthocyanins: A scientific exploration of sweet signals and natural pigments

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-31 DOI: 10.1016/j.plantsci.2025.112409

Ting Zhao , Qian Li , Ting Yan , Boping Yu , Qi Wang , Delu Wang

The presence of anthocyanins imparts vibrant hues to plants, whose biosynthesis and accumulation is a complex process and are influenced by numerous factors. In plants, sugar acts as a primary energy source and signaling molecule regulating anthocyanins biosynthesis. In this review, we provides a comprehensive overview of the relationship between sugar and anthocyanin. We delved into the intricate biosynthetic pathway of anthocyanins, outlining the key structural genes involved and their functions. Furthermore, we summarized how various environmental factors such as sugar, light, abiotic stresses, etc., affect anthocyanin biosynthesis. Notably, Most notably, we emphasized that sugars can independently regulate anthocyanin biosynthesis by modulating the expression of the MBW complex or structural genes, as well as through cascades involving hormones. These findings offer valuable insights into understanding the molecular mechanisms underlying anthocyanin accumulation and present potential avenues for enhancing anthocyanin content in plants through targeted manipulations that could have applications in agriculture and nutrition.

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引用次数: 0

A review of the interaction mechanisms between jasmonic acid (JA) and various plant hormones, as well as the core regulatory role of MYC2

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-31 DOI: 10.1016/j.plantsci.2025.112407

Tingting Mu , Shilei Luo , Long Li , Rongrong Zhang , Peng Wang , Guobin Zhang

Jasmonic acid (JA), as a defensive plant hormone, can synergistically or antagonistically interact with common hormones such as gibberellin (GA), abscisic acid (ABA), indole-3-acetic hormone acid (IAA), and ethylene (ETH) during the plant growth process, as well as interact with hormones such as melatonin (MT), brassinolide (BR), and resveratrol to regulate plant growth and development processes such as metabolite synthesis, pest and disease defense, and organ growth. The core regulatory factor MYC2 of JA mainly mediates the signal transduction pathways of these hormone interactions by interacting with other genes or regulating transcription. This article reviews the mechanism of cross-talk between JA and hormones such as ABA, GA, and salicylic acid (SA), and discusses the role of MYC2 in hormone interactions.

引用次数: 0

Unveiling the novel role of spermidine in leaf senescence: A study of eukaryotic translation factor 5A-independent and dependent mechanisms

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-31 DOI: 10.1016/j.plantsci.2025.112408

Ewelina Paluch-Lubawa , Kinga Popławska , Magdalena Arasimowicz-Jelonek , Ewa Sobieszczuk-Nowicka

Senescence is a crucial and highly active process in plants, optimising resource allocation and promoting phenotypic plasticity under restricted conditions. It involves global metabolic reprogramming for the organised disintegration and remobilization of resources. Polyamines (PAs) are polycationic biogenic amines prevalent in all eukaryotes and are necessary for cell survival. The commonly used PAs in plants include putrescine, spermidine, and spermine. Notably, the leaf's expression of S-adenosylmethionine decarboxylase and spermidine synthase gene family transcripts significantly changes during senescence. This suggests these genes are critical in spermidine metabolism and may condition metabolic reprogramming. One key role of spermidine in eukaryotes is to provide the 4-aminobutyl group for the posttranslational modification of lysine in eukaryotic translation factor 5A (eIF5A). This modification is catalysed by two sequential enzymatic steps leading to the activation of eIF5A by converting lysine to the unusual amino acid hypusine. Although eIF5A is well characterised to be involved in the translation of proline-rich repeat proteins and other hard-to-read motifs, the biological role of eIF5A has recently been clarified only in mammals. It could be better described at the plant functional level. The expression patterns of eIF5A isoforms and genes encoding machinery responsible for hypusination, differ between induced and developmental leaf senescence. In this paper, we summarise the existing knowledge on spermidine-dependent senescence control mechanisms in plants, raising the possibility that spermidine could be an element of a biological switch controlling the onset of a different type of senescence in an eIF5A-independent and dependent manner.

{"title":"Unveiling the novel role of spermidine in leaf senescence: A study of eukaryotic translation factor 5A-independent and dependent mechanisms","authors":"Ewelina Paluch-Lubawa , Kinga Popławska , Magdalena Arasimowicz-Jelonek , Ewa Sobieszczuk-Nowicka","doi":"10.1016/j.plantsci.2025.112408","DOIUrl":"10.1016/j.plantsci.2025.112408","url":null,"abstract":"<div><div>Senescence is a crucial and highly active process in plants, optimising resource allocation and promoting phenotypic plasticity under restricted conditions. It involves global metabolic reprogramming for the organised disintegration and remobilization of resources. Polyamines (PAs) are polycationic biogenic amines prevalent in all eukaryotes and are necessary for cell survival. The commonly used PAs in plants include putrescine, spermidine, and spermine. Notably, the leaf's expression of S-adenosylmethionine decarboxylase and spermidine synthase gene family transcripts significantly changes during senescence. This suggests these genes are critical in spermidine metabolism and may condition metabolic reprogramming. One key role of spermidine in eukaryotes is to provide the 4-aminobutyl group for the posttranslational modification of lysine in eukaryotic translation factor 5A (eIF5A). This modification is catalysed by two sequential enzymatic steps leading to the activation of eIF5A by converting lysine to the unusual amino acid hypusine. Although eIF5A is well characterised to be involved in the translation of proline-rich repeat proteins and other hard-to-read motifs, the biological role of eIF5A has recently been clarified only in mammals. It could be better described at the plant functional level. The expression patterns of <em>eIF5A</em> isoforms and genes encoding machinery responsible for hypusination, differ between induced and developmental leaf senescence. In this paper, we summarise the existing knowledge on spermidine-dependent senescence control mechanisms in plants, raising the possibility that spermidine could be an element of a biological switch controlling the onset of a different type of senescence in an eIF5A-independent and dependent manner.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112408"},"PeriodicalIF":4.2,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accumulation of theanine in tea plant (Camellia sinensis (L.) O. Kuntze): Biosynthesis, transportation and strategy for improvement

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-31 DOI: 10.1016/j.plantsci.2025.112406

Qianting Luo , Hua-Feng He

Theanine, specifically biosynthesized and accumulated in Camellia sinensis (L.) O. Kuntze, is widely recognized as the most positive ingredient related to the quality of tea. Therefore, genetic factors related to the biosynthesis of theanine in tea plant, CsAlaDC, CsGGTs and CsMYBs, etc., were elaborated and proved to be influential. Oppositely, TFs acting on the growth and development of tea plants, CsPIF, CsHO as well as CsGDH were demonstrated to be negative for biosynthesis of theanine. Since root is the original assembly site, transportation is indispensable for the accumulation of theanine in leaf. CsAAP7.2 was elucidated to be involved in the transportation of theanine crossing the vascular system to vegetative tissues. In order to promote the accumulation of theanine, strategies were proposed in aspect of processing, cultivation, fertilizer as well as germplasm innovation. Appropriate processing technology, scientific planting manner and fertilizer application, coupling with domestication of excellent varieties portrayed out the future orientation of theanine. Purpose of the review was to summarize advantages achieved in related to metabolism of theanine, and to motivate more intensive and more effective means to promote the accumulation of theanine in tea plant.

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引用次数: 0

An assay for assessing 1-aminocyclopropane-1-carboxylate malonyl (MACC) transferase (AMT) activity and its regulation by ethylene

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-30 DOI: 10.1016/j.plantsci.2025.112401

J. Pattyn , M. Geerts Danau , D. De Ruysscher , S. Roden , T. Snoekx , J. Masschelein , J. Vaughan-Hirsch , B. Van de Poel

Background

N-malonyl 1-aminocyclopropane-1-carboxylic acid (MACC) is a major conjugate of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and may therefore play an important role in regulating ethylene production, as well as ethylene-independent ACC signalling. While the enzyme responsible for this derivatization, ACC malonyltransferase (AMT), has been studied in the past, its identity remains unknown. Methods to assay AMT activity are not well established, and no standardized assay has been described.

Results

We optimized an AMT activity assay and investigated the biological implications of AMT. This assay can be divided into three parts: total protein extraction, in vitro AMT activity assay, and MACC detection. For these three parts, different parameters were optimized and combined into an integrated and robust protocol. We used gas chromatography for the indirect detection of MACC, which was compared to a direct LC-MS approach, indicating that the GC-based method is a good alternative readily available to most labs studying ethylene. Next, we used this in vitro AMT activity assay to study the biological function of MACC formation. We observed an ontogenetic, tissue-specific and an ethylene-mediated feedback effect on AMT activity in tomato and Arabidopsis. The feedback of ethylene on AMT activity seems to be important to regulate ethylene production levels.

Conclusions

The optimized and robust AMT activity assay presented here will enable other plant researchers to investigate the biochemistry of the ethylene biosynthesis pathway through ACC conjugation into MACC. Our AMT activity method was deployed both in tomato and Arabidopsis, and revealed that AMT activity is tightly controlled by ethylene itself in a tissue-specific way.

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引用次数: 0

The MdWRKY17 positively regulates nitrate uptake by promoting MdNRT2.5 expression under long-term low N stress in apple

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-30 DOI: 10.1016/j.plantsci.2025.112402

Zehui Hu, Dongqian Shan, Chanyu Wang, Yixue Bai, Tianci Yan, Tong Zhang, Handong Song, Ruoxue Li, Yixuan Zhao, Qian Deng, Changjian Dai, Peiyun Xiao, Silong Dong, Jin Kong

Nitrogen (N) supply is critical for apple yield and quality. Improving nitrogen use efficiency (NUE) could reduce fertilizer application for maintaining apple yield at the cost of environmental pollution in infertile soil. The molecular mechanisms underlying nitrate (NO₃^-) uptake are foundational for breeding high NUE cultivars. The two-month low N treatment mimicking infertile soil dramatically induced the accumulation of transcription factor MdWRKY17 in apple. Overexpression of MdWRKY17 conferred enhanced long-term low nitrogen tolerance in transgenic apple plants and calli, while RNA interference of MdWRKY17 reduced this tolerance. MdNRT2.5 encoding a high-affinity nitrate transporter was identified by chromatin immunoprecipitation sequencing (ChIP-seq) as the direct target of MdWRKY17. This is confirmed by in vitro EMSA and in vivo ChIP-qPCR assay. Notably, overexpression of MdNRT2.5 increased NO₃^- uptake under long-term N-deficiency conditions. RNA interference of MdNRT2.5 in roots decreased NO₃^- uptake efficiency of MdWRKY17-OE transgenic apple plants, indicating that MdWRKY17 improves NO₃^- uptake mainly by activating MdNRT2.5 expression. Our study identified an important MdWRKY17-MdNRT2.5 module in response to long-term low N stress, which will contribute to the molecular breeding of high NUE apple cultivars.

{"title":"The MdWRKY17 positively regulates nitrate uptake by promoting MdNRT2.5 expression under long-term low N stress in apple","authors":"Zehui Hu, Dongqian Shan, Chanyu Wang, Yixue Bai, Tianci Yan, Tong Zhang, Handong Song, Ruoxue Li, Yixuan Zhao, Qian Deng, Changjian Dai, Peiyun Xiao, Silong Dong, Jin Kong","doi":"10.1016/j.plantsci.2025.112402","DOIUrl":"10.1016/j.plantsci.2025.112402","url":null,"abstract":"<div><div>Nitrogen (N) supply is critical for apple yield and quality. Improving nitrogen use efficiency (NUE) could reduce fertilizer application for maintaining apple yield at the cost of environmental pollution in infertile soil. The molecular mechanisms underlying nitrate (NO<sub>3</sub><sup>-</sup>) uptake are foundational for breeding high NUE cultivars. The two-month low N treatment mimicking infertile soil dramatically induced the accumulation of transcription factor MdWRKY17 in apple. Overexpression of <em>MdWRKY17</em> conferred enhanced long-term low nitrogen tolerance in transgenic apple plants and calli, while RNA interference of <em>MdWRKY17</em> reduced this tolerance. <em>MdNRT2.5</em> encoding a high-affinity nitrate transporter was identified by chromatin immunoprecipitation sequencing (ChIP-seq) as the direct target of MdWRKY17. This is confirmed by <em>in vitro</em> EMSA and <em>in vivo</em> ChIP-qPCR assay. Notably, overexpression of <em>MdNRT2.5</em> increased NO<sub>3</sub><sup>-</sup> uptake under long-term N-deficiency conditions. RNA interference of <em>MdNRT2.5</em> in roots decreased NO<sub>3</sub><sup>-</sup> uptake efficiency of <em>MdWRKY17</em>-OE transgenic apple plants, indicating that MdWRKY17 improves NO<sub>3</sub><sup>-</sup> uptake mainly by activating <em>MdNRT2.5</em> expression. Our study identified an important <em>MdWRKY17-MdNRT2.5</em> module in response to long-term low N stress, which will contribute to the molecular breeding of high NUE apple cultivars.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112402"},"PeriodicalIF":4.2,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Volatile signaling in weed plant Ageratina adenophora: Understanding the key emissions influencing Procecidochares utilis attraction to gall formation

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-30 DOI: 10.1016/j.plantsci.2025.112404

Nipapan Kanjana , Yuyan Li , Muhammad Afaq Ahmed , Lin Ma , Lisheng Zhang

The stem gall fly (Procecidochares utilis) significantly impacts host–plant biology by inhabiting specific parts of stem tissue, ensuring its own survival. Despite this, comprehensive identification of the primary bioactive compounds within host plants that are involved in gall formation remains elusive. This study aims to elucidate the crucial volatile compounds utilized by gall flies to alter host–plant defenses, either through direct or indirect manipulation via the release of an enticing volatile compound attractive to the fly. Employing Y-tube olfactometer assays, we examined the response of Procecidochares utilis to host plants from three Asteraceae weed species—Ageratina adenophora, Ageratum conyzoides, and Praxelis clematidea. Volatile compounds were extracted using headspace solid-phase microextraction (HS-SPME) and SPME-FIBER. Subsequently, gas chromatography-electroantennography and electroantennography were employed to analyze the antennal responses to individual odorants. The analysis revealed that the primary bioactive compound varied among the three weed species. Out of a total of 805 known volatiles, 65 main active compounds were exclusive to Ageratina adenophora (host plant). Remarkably, only 8 bioactive compounds were identified to elicit an antennal response from Procecidochares utilis. Notably, caryophyllene, β-bisabolene, and 4-thujen-2-α-yl acetate exhibited the remarkable ability to elicit an attraction response from both sexes of Procecidochares utilis. Among these, β-bisabolene emerged as the key compound, eliciting the most significant response from the gall fly antenna. Our findings offer novel insights into the specific attraction of the stem gall fly to Ageratina adenophora, utilizing key odorants as unique cues for initiating gall formation on its host plant. This discovery highlights how these cues enable the gall fly to exert direct or indirect control over its host. Additionally, these findings underscore the potential of this approach in the development of sustainable pest management strategies in the context of field trials.

{"title":"Volatile signaling in weed plant Ageratina adenophora: Understanding the key emissions influencing Procecidochares utilis attraction to gall formation","authors":"Nipapan Kanjana , Yuyan Li , Muhammad Afaq Ahmed , Lin Ma , Lisheng Zhang","doi":"10.1016/j.plantsci.2025.112404","DOIUrl":"10.1016/j.plantsci.2025.112404","url":null,"abstract":"<div><div>The stem gall fly (<em>Procecidochares utilis</em>) significantly impacts host–plant biology by inhabiting specific parts of stem tissue, ensuring its own survival. Despite this, comprehensive identification of the primary bioactive compounds within host plants that are involved in gall formation remains elusive. This study aims to elucidate the crucial volatile compounds utilized by gall flies to alter host–plant defenses, either through direct or indirect manipulation via the release of an enticing volatile compound attractive to the fly. Employing Y-tube olfactometer assays, we examined the response of <em>Procecidochares utilis</em> to host plants from three Asteraceae weed species—<em>Ageratina adenophora</em>, <em>Ageratum conyzoides</em>, and <em>Praxelis clematidea</em>. Volatile compounds were extracted using headspace solid-phase microextraction (HS-SPME) and SPME-FIBER. Subsequently, gas chromatography-electroantennography and electroantennography were employed to analyze the antennal responses to individual odorants. The analysis revealed that the primary bioactive compound varied among the three weed species. Out of a total of 805 known volatiles, 65 main active compounds were exclusive to <em>Ageratina adenophora</em> (host plant). Remarkably, only 8 bioactive compounds were identified to elicit an antennal response from <em>Procecidochares utilis</em>. Notably, caryophyllene, β-bisabolene, and 4-thujen-2-α-yl acetate exhibited the remarkable ability to elicit an attraction response from both sexes of <em>Procecidochares utilis</em>. Among these, β-bisabolene emerged as the key compound, eliciting the most significant response from the gall fly antenna. Our findings offer novel insights into the specific attraction of the stem gall fly to <em>Ageratina adenophora</em>, utilizing key odorants as unique cues for initiating gall formation on its host plant. This discovery highlights how these cues enable the gall fly to exert direct or indirect control over its host. Additionally, these findings underscore the potential of this approach in the development of sustainable pest management strategies in the context of field trials.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112404"},"PeriodicalIF":4.2,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The 14–3–3 gene AaGRF1 positively regulates cold tolerance in kiwifruit

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-29 DOI: 10.1016/j.plantsci.2025.112403

Zhang Zhenzhen , Li Sumei , Sun Shihang , Li Hongli , Zhang Qina , Li Yihang , Li Yukuo , Liu Mingyu , Li Congcong , Sun Leiming , Lin Miaomiao , Qi Xiujuan

Low temperatures severely threaten the growth and development of kiwifruit. Research has demonstrated that proteins belonging to the 14–3–3 family play a pivotal regulatory function in the ability of plants to resist stress. However, this specific roles of the genes in kiwifruit cold tolerance remain unclear. It had been identified that β-amylase gene, AaBAM3.1, exhibits a positive regulatory effect on kiwifruit's tolerance to low temperature. In our research, we obtained the Actinidia arguta 14–3–3 gene general regulatory factor 1 (AaGRF1) from yeast one-hybrid (Y1H) screening library of the AaBAM3.1 promoter; the expression level of AaGRF1 was enhanced by low-temperature stress. Subcellular localization, Y1H and dual-LUC assay indicated that the AaGRF1 protein resides within the nucleus and possesses the ability to interact with the AaBAM3.1 promoter. Moreover, we also studied the role of AaGRF1 gene in cold resistance of kiwifruit. When AaGRF1 was overexpressed in kiwifruit, the transgenic plants exhibited enhanced cold tolerance. The level of antioxidants and soluble sugars in these plants were elevated compared to wild-type (WT) lines. RNA-seq of the transgenic and WT lines revealed that AaGRF1 might interact with genes in the ‘ascorbate-glutathione’ and ‘starch and sucrose’ pathways, thereby enhancing the cold resistance of kiwifruit. In summary, we hypothesize that the 14–3–3 gene AaGRF1 may positively modulate the cold resistance in kiwifruit by accumulating more antioxidants and soluble sugars.

{"title":"The 14–3–3 gene AaGRF1 positively regulates cold tolerance in kiwifruit","authors":"Zhang Zhenzhen , Li Sumei , Sun Shihang , Li Hongli , Zhang Qina , Li Yihang , Li Yukuo , Liu Mingyu , Li Congcong , Sun Leiming , Lin Miaomiao , Qi Xiujuan","doi":"10.1016/j.plantsci.2025.112403","DOIUrl":"10.1016/j.plantsci.2025.112403","url":null,"abstract":"<div><div>Low temperatures severely threaten the growth and development of kiwifruit. Research has demonstrated that proteins belonging to the 14–3–3 family play a pivotal regulatory function in the ability of plants to resist stress. However, this specific roles of the genes in kiwifruit cold tolerance remain unclear. It had been identified that β-amylase gene, <em>AaBAM3.1</em>, exhibits a positive regulatory effect on kiwifruit's tolerance to low temperature. In our research, we obtained the <em>Actinidia arguta</em> 14–3–3 gene general regulatory factor 1 (<em>AaGRF1</em>) from yeast one-hybrid (Y1H) screening library of the <em>AaBAM3.1</em> promoter; the expression level of <em>AaGRF1</em> was enhanced by low-temperature stress. Subcellular localization, Y1H and dual-LUC assay indicated that the AaGRF1 protein resides within the nucleus and possesses the ability to interact with the <em>AaBAM3.1</em> promoter. Moreover, we also studied the role of <em>AaGRF1</em> gene in cold resistance of kiwifruit. When <em>AaGRF1</em> was overexpressed in kiwifruit, the transgenic plants exhibited enhanced cold tolerance. The level of antioxidants and soluble sugars in these plants were elevated compared to wild-type (WT) lines. RNA-seq of the transgenic and WT lines revealed that <em>AaGRF1</em> might interact with genes in the ‘ascorbate-glutathione’ and ‘starch and sucrose’ pathways, thereby enhancing the cold resistance of kiwifruit. In summary, we hypothesize that the 14–3–3 gene <em>AaGRF1</em> may positively modulate the cold resistance in kiwifruit by accumulating more antioxidants and soluble sugars.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112403"},"PeriodicalIF":4.2,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0