Preventive veterinary medicine最新文献

{"title":"Mapping risks: A value chain approach to brucellosis introduction in Zhijiang's Cattle Population, China","authors":"Zihan Tian ,&nbsp;Sheng Jiang ,&nbsp;Peng Yuan ,&nbsp;Xiaozhong Wang ,&nbsp;Jie Pei ,&nbsp;Hao Tang ,&nbsp;Shubo Li ,&nbsp;Ian D. Robertson ,&nbsp;Aizhen Guo ,&nbsp;Yingyu Chen","doi":"10.1016/j.prevetmed.2025.106467","DOIUrl":"10.1016/j.prevetmed.2025.106467","url":null,"abstract":"<div><div>Brucellosis, a zoonotic disease primarily transmitted through livestock, poses significant health and economic challenges globally. Its increasing incidence in China highlights critical gaps in current control measures, particularly in regions with high livestock movement, where domestic ruminants trade networks elevate the risk of disease transmission. Traditional value chain analyses often provide descriptive mappings of these networks but lack quantitative methods to assess specific risk levels. This study integrates value chain with quantitative risk assessment using Monte Carlo simulations to evaluate the risk of brucellosis introduction via cattle imports in Zhijiang. We comprehensively mapped the local cattle value chain, identifying key stakeholders, risk nodes, and critical pathways of infection. Utilizing a stochastic scenario tree model based on local data, we estimated the likelihood of infected cattle entering the region, revealing that unregulated brokers and inadequate quarantine measures significantly contribute to the risk. Our sensitivity analysis confirmed vulnerabilities in existing biosecurity protocols, emphasizing the need for enhanced quarantine measures and reliable testing at high-risk points in cattle trade. By combining value chain insights with quantitative analysis, this study effectively identifies critical vulnerabilities within livestock networks. Our findings lay the groundwork for developing targeted brucellosis prevention strategies in Zhijiang, underscoring the urgent need for strengthened biosecurity and risk management practices.</div></div>","PeriodicalId":20413,"journal":{"name":"Preventive veterinary medicine","volume":"238 ","pages":"Article 106467"},"PeriodicalIF":2.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global prevalence of Porcine Astrovirus: A systematic review and meta-analysis","authors":"Xiaoyu Ge ,&nbsp;Yize Li ,&nbsp;Feipeng Zhao ,&nbsp;Xin’ao Ma ,&nbsp;Jiaxuan Li ,&nbsp;Yanping Jiang ,&nbsp;Wen Cui ,&nbsp;Xiaona Wang ,&nbsp;Lijie Tang","doi":"10.1016/j.prevetmed.2025.106465","DOIUrl":"10.1016/j.prevetmed.2025.106465","url":null,"abstract":"<div><div><em>Porcine astrovirus</em> (PAstV) is a widespread enteric virus in pigs, often co-infecting with other enteric viruses and contributing to diarrhea, leading to significant economic losses in the global swine industry. This study aims to identify the factors influencing PAstV prevalence by conducting a statistical meta-analysis of global data and evaluating heterogeneity across various subgroups. A systematic literature search was performed across PubMed, Web of Science, CNKI, Wanfang, and VIP databases, covering studies from the inception of the databases up to December 2023. A random-effects model was employed to estimate the global pooled prevalence of PAstV infection, and subgroup analyses were performed to evaluate the impact of different continents, years, detection methods, and sample types on the prevalence. Publication bias was assessed using a funnel plot and Egger's test. A total of 45 studies from 10 countries across three continents, involving 376 articles, were included in the meta-analysis. The global pooled prevalence of PAstV infection was found to be 28.19 % (95 % CI, 21.94 %-34.89 %). Subgroup analysis indicated significant differences in PAstV prevalence across continents, with Asia at 26.25 % (95 % CI, 25.41 %-27.09 %), Europe at 36.19 % (95 % CI, 34.09 %-38.33 %), and North America at 63.24 %. The prevalence of PAstV was highest between 2012 and 2014 (49.86 %, 95 % CI, 47.21 %-52.51 %), followed by a decreasing trend that stabilized below 30 % from 2015 to 2023. The analysis showed no significant influence of detection methods on PAstV prevalence. However, the prevalence in non-fecal samples (43.09 %, 95 % CI: 41.05 %-45.15 %) was significantly higher than in fecal samples (22.92 %, 95 % CI: 21.87 %-23.99 %). Additionally, the prevalence of PAstV in asymptomatic pigs (36.71 %, 95 % CI, 34.97 %-38.48 %) exceeded that in diarrheic pigs (28.18 %, 95 % CI, 26.94 %-29.44 %). Among different age groups, nursery pigs(6–10 weeks) exhibited the highest infection rate (63.19 %, 95 % CI, 58.45 %-67.75 %), followed by weaning pigs(3–6 weeks) (60.00 %, 95 % CI, 56.48 %-63.45 %), finisher pigs(&gt;10 weeks) (49.89 %, 95 % CI, 46.59 %-53.19 %), sows (35.33 %, 95 % CI, 31.45 %-39.37 %), with suckling pigs(0–3 weeks) showing the lowest rate (31.93 %, 95 % CI, 30.23 %-33.68 %). This study highlights the widespread nature of PAstV infection in pig populations globally, with notable variations in prevalence across regions, years, and sample types. The high prevalence of asymptomatic infections underscores the need for enhanced PAstV surveillance and control measures.</div></div>","PeriodicalId":20413,"journal":{"name":"Preventive veterinary medicine","volume":"238 ","pages":"Article 106465"},"PeriodicalIF":2.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel characterisation of dairy herds in Wales: A description of principal herd typologies and antimicrobial use patterns","authors":"Caroline M. Best ,&nbsp;Lucy Vass ,&nbsp;Elliot Stanton ,&nbsp;Judy Bettridge ,&nbsp;Andrew W. Dowsey ,&nbsp;Kristen K. Reyher","doi":"10.1016/j.prevetmed.2025.106460","DOIUrl":"10.1016/j.prevetmed.2025.106460","url":null,"abstract":"<div><div>Antimicrobial resistance (AMR) is one of the top global public health concerns. Reducing and refining antimicrobial use (AMU) in farmed livestock is vital in slowing the development of AMR and preserving the efficacy of antimicrobials (AMs) in both humans and animals. Understanding the risk factors for AMU, however, is crucial to informing sustainable and effective farm AMU reduction and prudent use strategies. As a range of farm-level variables are likely to impact AMU, multidimensional exploratory analyses play a pivotal role in identifying direct and indirect factors influencing variation in AMU typically observed between dairy herds. This study used exploratory approaches to investigate whether typologies of dairy herds could be determined on the basis of farm characteristics, health parameters and management practices, and whether these herd types were differentiated by AMU. This study was conducted on 21 dairy herds in Wales, United Kingdom. Comprehensive surveys were administered and 224 usable variables regarding farm characteristics, herd health parameters and management practices were collated. AM sales data for each herd were used as a proxy for AMU. Multiple correspondence analysis (MCA) and hierarchical clustering on principal components (HCPC) were performed. The top 10 dimensions yielded by MCA explained 65.7 % of the total variance. Two data-driven typologies of dairy herds, produced from the first two cut-points of the HCPC dendrogram, were visualised and described. Here, five partitions of relatively homogeneous herds (herd types) were characterised and contrasted by 73 variable categories. Herd types were primarily constructed by variables focused on drying-off practices (and use of intramammary [IMM] AMs), herd size, stock purchasing and culling rates in addition to those concerning husbandry, disease management, grazing practices and veterinarian contact. Herd types characterised by performing blanket dry cow therapy (BDCT) used a higher mass of dry cow IMM AMs, EMA Category C and B AMs and had higher medium total AMU (mg/PCU) compared to herd types characterised by performing selective dry cow therapy (SDCT). From this study help untangle the myriad of factors influencing AMU at herd level and provide insight into the challenges of good AM stewardship. Strategies for sustainable reductions in AMU should be directed toward specific herd types identified, such as targeted interventions to implement SDCT. Multivariate exploratory approaches of dimensionality reduction and clustering are invaluable in elucidating the risk factors for AMU when utilising high-dimensional datasets. Future prospective studies are needed to validate herd types and confirm causality of findings.</div></div>","PeriodicalId":20413,"journal":{"name":"Preventive veterinary medicine","volume":"238 ","pages":"Article 106460"},"PeriodicalIF":2.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serosurvey of Leishmania infantum in equids in different European countries","authors":"Jesús Barbero-Moyano ,&nbsp;David Cano-Terriza ,&nbsp;Moisés Gonzálvez ,&nbsp;Inmaculada Moreno ,&nbsp;Eduard Jose-Cunilleras ,&nbsp;Francesco Buono ,&nbsp;Vincenzo Veneziano ,&nbsp;Eduardo Alguacil ,&nbsp;Jesús García ,&nbsp;Fabrizia Veronesi ,&nbsp;María A. Risalde ,&nbsp;Ignacio García-Bocanegra","doi":"10.1016/j.prevetmed.2025.106463","DOIUrl":"10.1016/j.prevetmed.2025.106463","url":null,"abstract":"<div><div>Leishmaniosis is a vector-borne and zoonotic disease with major sanitary implications at global scale. In Europe, <em>Leishmania infantum</em> is the only endemic etiologic agent, which has been previously reported in a wide range of mammal. However, the information about the role of equids in the <em>L. infantum</em> epidemiology is limited. We aimed to assess the seroprevalence and identify potential risk factors associated with <em>L. infantum</em> seropositivity in equine populations across several European countries. A total of 1364 equids, including 1005 horses, 240 donkeys and 119 mules/hinnies from Spain, Italy, United Kingdom and Ireland were sampled between 2011 and 2023. The overall seroprevalence of <em>L. infantum</em> was 9.8 % using indirect immunofluorescence test. A significant higher prevalence of anti-<em>L. infantum</em> antibodies was found in donkeys (27.9 %) and mules/hinnies (21.1 %) compared to horses (4.0 %). By country, significantly higher seropositivity was detected in equids from Italy (17.7 %; 70/395) compared to Spain (11.2 %; 63/561), while no positivity was found in animals from Ireland and UK. Our results indicate a moderate and heterogeneous spatial circulation of <em>L. infantum</em> in equids from southern Europe. The variable ‘species’ (donkeys and mules/hinnies) was identified as risk factor associated with <em>L. infantum</em> seropositivity. This survey constitutes the largest seroepidemiological study evaluating the circulation of <em>L. infantum</em> in equids at global scale. Additionally, we report for the first time <em>L. infantum</em> exposure in mules/hinnies in Europe. Our findings suggest the potential role of equids in the <em>L. infantum</em> epidemiological cycle in southern Europe and the need to implement specific surveillance programs in these species.</div></div>","PeriodicalId":20413,"journal":{"name":"Preventive veterinary medicine","volume":"238 ","pages":"Article 106463"},"PeriodicalIF":2.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human-animal interactions, occupational health and well-being in pig slaughterhouses of Colombia: Exploring worker perceptions and practices","authors":"Adriana P. Pastrana-Camacho ,&nbsp;Genaro C. Miranda-de la Lama ,&nbsp;Laura X. Estévez-Moreno","doi":"10.1016/j.prevetmed.2025.106462","DOIUrl":"10.1016/j.prevetmed.2025.106462","url":null,"abstract":"<div><div>Public concern about global health has forced the meat industry to make technological, operational and logistical improvements, but human-animal interactions are rarely considered from an animal welfare perspective. A total of 171 workers from 12 pig slaughterhouses were interviewed about their occupational perceptions and practices affecting their health and well-being, and human-pig interactions. Ninety percent of the workers interviewed were male, between 18 and 30 years old, with high school education, &lt; 7 years of experience, and learned their work in the slaughterhouse. In terms of human-animal interactions, we found that the nature of the tasks performed by each worker had an impact on their perceptions of pigs, particularly those involving work with live animals (outdoors) and stunning and bleeding (indoors). In addition, respondents show disapproval of cruel practices and a high level of awareness of pig pain and concern to alleviate it. However, there are neutral responses to statements that show some weakness in their feelings towards pigs. Forty per cent of respondents reported having at least one of the health problems and had suffered occupational accidents. The most common types of accidents were falls, cuts and animal aggression. These accidents were related to incorrect movements, especially in the morning, by personnel &gt; 40 years of age, with &gt; 7 years of experience, and the upper and/or lower extremities were most affected. Our results indicate that fatigue caused by factors such as line speed, uncoordinated operations, and risky behavior by workers, is the main cause of workplace accidents in slaughterhouses. Based on this evidence, we conclude that adjustments of work break/rest intervals and improvements of working conditions are necessary to reduce fatigue, improve safety, and enhance work efficiency.</div></div>","PeriodicalId":20413,"journal":{"name":"Preventive veterinary medicine","volume":"238 ","pages":"Article 106462"},"PeriodicalIF":2.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of early detection surveillance in PRRS-free regions","authors":"Mira Willkan ,&nbsp;Lorraine Chapot ,&nbsp;Angus Cameron ,&nbsp;Søren Kjærgaard Boldsen ,&nbsp;Mette Fertner","doi":"10.1016/j.prevetmed.2025.106461","DOIUrl":"10.1016/j.prevetmed.2025.106461","url":null,"abstract":"<div><div>In May 2022, a national control program targeting porcine reproductive and respiratory syndrome virus was launched in Denmark, and the first regions are approaching a PRRS-free status. Hence, the question now arises as to how the surveillance should be performed to identify new incursions of the PRRS virus in PRRS-free regions as early as possible. The aim of the present study was to quantify the early detection sensitivity of the current and alternative PRRS surveillance systems in Denmark at a regional level. The current PRRS surveillance system is composed of a notifiable surveillance component and an active serological surveillance component, consisting of monthly sampling (breeding and multiplier farms) or yearly sampling (production farms). The results from the present study estimated that the current surveillance system would have a 21.5 % [16.0 %;32.4 %]_min;max probability of detecting an incursion of PRRS into a PRRS-free region. If monthly serum samples were taken on all farms, the probability would be increased to 87.7 % [87.4 %;88.6 %]_min;max. Adding a syndromic surveillance component to the existing surveillance system resulted in an early detection sensitivity of 23.9 % [17.6 %;34.6 %]_min;max, while targeted risk-based sampling resulted in probabilities of 27.8 % [17.9 %;33.8 %]_min;max and 62.6 % [17.9 %;79.5 %]_min;max for quarterly or monthly sampling frequencies, respectively. Results from the present study indicate a relatively low chance of detecting newly infected farms within the first month in a PRRS-free region with the current surveillance system. The probability of early detection of PRRS can be increased either by increasing the sampling frequency on all farms or by using targeted risk-based sampling.</div></div>","PeriodicalId":20413,"journal":{"name":"Preventive veterinary medicine","volume":"238 ","pages":"Article 106461"},"PeriodicalIF":2.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SVEPM 2024, the annual conference of the Society for Veterinary Epidemiology and Preventive Medicine, Uppsala, Sweden","authors":"Fernanda C. Dórea,&nbsp;Carla Correia-Gomes,&nbsp;Luís Pedro Carmo,&nbsp;Egil A.J. Fischer,&nbsp;Locksley L. McV. Messam,&nbsp;Katja Schulz,&nbsp;Timothée Vergne,&nbsp;Dave C. Brodbelt","doi":"10.1016/j.prevetmed.2025.106458","DOIUrl":"10.1016/j.prevetmed.2025.106458","url":null,"abstract":"","PeriodicalId":20413,"journal":{"name":"Preventive veterinary medicine","volume":"237 ","pages":"Article 106458"},"PeriodicalIF":2.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue cyst-based quantitative risk assessment of human toxoplasmosis from consuming pork in Denmark: A farm-to-fork approach","authors":"Abbey Olsen ,&nbsp;Arno Swart ,&nbsp;Sara Monteiro Pires ,&nbsp;Sisse Fagt ,&nbsp;Vibeke Møgelmose ,&nbsp;Hans Houe ,&nbsp;Matthew Denwood ,&nbsp;Henrik Vedel Nielsen ,&nbsp;Lis Alban","doi":"10.1016/j.prevetmed.2025.106446","DOIUrl":"10.1016/j.prevetmed.2025.106446","url":null,"abstract":"<div><div><em>Toxoplasma gondii</em> is an important foodborne pathogen. The aim of this study was to develop a tissue cyst-based quantitative risk assessment model to estimate the probability of human infection with <em>T. gondii</em> from consuming pork in Denmark. A 'farm-to-fork' model was developed to trace the path of <em>T. gondii</em> infection, beginning with the true seroprevalence in pigs and proceeding to the estimation of tissue cysts in portions of pork. The model then accounted for the number of infectious portions post preparation and prior to consumption. Thereafter, the model estimated the probability of human infection from consumption of infectious portions using a dose-response model. The model predicted the prevalence of <em>T. gondii</em> infections in humans, with the assumption that infections did not equate clinical illness. The average estimated prevalence at the age of 75 years ranged from 58.8% to 88.8%, depending on specific model scenarios. Furthermore, the predicted prevalence was higher for portions originating from conventionally raised pigs compared to those from organic pigs. This was due to the significantly higher proportion of consumption of conventional pork compared to organic pork, and despite the per-portion risk being higher for organic pork. The model identified that undercooked pork, dry-cured sausages, and smoked products pose a risk of infection. The probabilities of human infection per portion consumed were estimated at 7.3 × 10⁻⁴, 4.7 × 10⁻⁴, and 1.2 × 10⁻⁵, respectively. However, the actual risk posed by dry-cured sausages remains uncertain due to limited data on the effectiveness of salting applied in dry-cured sausage processing. It was epidemiologically challenging to build the model due to the lack of comprehensive data on salting processes and the complexity of accurately reflecting industry standards. The age-specific prevalence offers limited value for public health decision-making; however, attribution of risk to specific pork products and in comparing organic and conventional pork provides more actionable insights for the future.</div></div>","PeriodicalId":20413,"journal":{"name":"Preventive veterinary medicine","volume":"238 ","pages":"Article 106446"},"PeriodicalIF":2.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Navigating the controversy: Public perspectives on free-roaming cat populations and control strategies in Israel","authors":"Idit Gunther,&nbsp;Doron Levin,&nbsp;Eyal Klement","doi":"10.1016/j.prevetmed.2025.106448","DOIUrl":"10.1016/j.prevetmed.2025.106448","url":null,"abstract":"<div><div>The debate surrounding the management of free-roaming cat populations is multifaceted, with conflicting views between conservationists and cat advocates. In Israel, which is characterized by densely populated free-roaming cat communities, cat numbers remain high despite extensive Trap-Neuter-Return (TNR) efforts. Recognizing the importance of public engagement for ensuring success of control campaigns, we aimed to gather public opinion on the free-roaming cat phenomenon and control strategies. Interviews were conducted with 700 randomly selected passersby at central bus and train stations nationwide. A notable fraction (223, 31.9 %) of the participants reported feeding free-roaming cats at least once during the month preceding the survey. Two sub-groups of dedicated feeders were identified: eighty feeders (11.4 %) who reported feeding on a daily basis, and forty-one (5.9 %) ‘heavy’ feeders who reported feeding more than ten cats. While feeders and non-feeders of free-roaming cats shared similar characteristics, daily feeders were generally older, and fewer were Israeli-born. 54 % of all participants and 45 % of the dedicated feeders considered free-roaming cats as a negative phenomenon. However, feeders typically regarded feeding free-roaming cats as beneficial (75 % strongly agreed with feeding free-roaming cats), while non-feeders exhibited a more ambivalent attitude (30 % disagreed and 35 % strongly agreed with feeding actions). Remarkably, both feeders and non-feeders agreed that the numbers of free-roaming cats should be reduced (77.3 % of all participants). In addition, most participants (62 % of all participants) agreed at least partially with the application of neutering as a cat population management tool, while the vast majority (90 % of all participants) rejected the idea of culling. In conclusion, the general public perceives free-roaming cats negatively, and as a consequence, there is widespread support for the necessity to reduce cat numbers. However, the strong association between abundance of vital resources, such as food provided by feeders, and the cat population dynamics, is not understood or disregarded by both feeder and non-feeders of free-roaming cats. Authorities should therefore consider combining cat control programs with public education campaigns to raise public awareness of this issue.</div></div>","PeriodicalId":20413,"journal":{"name":"Preventive veterinary medicine","volume":"237 ","pages":"Article 106448"},"PeriodicalIF":2.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of porcine epidemic diarrhea virus RNA contamination on swine industry transportation vehicles","authors":"Taylor B. Parker,&nbsp;Kelly A. Meiklejohn,&nbsp;Gustavo Machado,&nbsp;Michael Rahe,&nbsp;Bradford Sean Darrow,&nbsp;Juliana Bonin Ferreira","doi":"10.1016/j.prevetmed.2025.106447","DOIUrl":"10.1016/j.prevetmed.2025.106447","url":null,"abstract":"<div><div>Porcine epidemic diarrhea virus (PEDV) is one of the most devastating pathogens of global swine health and welfare. It is well known that contaminated fomites and vehicle movements play an important role in farm-to-farm PEDV spread, but the efficacy of cleaning and disinfection (C&amp;D) protocols on the reduction in dissemination risk via vehicles and trailers remains unclear. This study used swine industry data to determine how frequently vehicles and trailers were contaminated with PEDV RNA before and after C&amp;D. Environmental RNA samples were collected at three eastern North Carolina C&amp;D sites from four different vehicle types: crew trucks, feed trucks, pigs-to-farm trucks and trailers, and pigs-to-market trucks and trailers. A total of 2004 samples were collected from truck cabins, trailers, and tires before and after C&amp;D with two commercial disinfectants at two different concentrations. An in-house RT-qPCR assay was used to detect the presence of PEDV RNA only (not infectivity status). Results suggest that pigs-to-market trucks hauling live pigs were the most likely to be contaminated with PEDV (79.17 % of trailers tested positive before C&amp;D and 88.52 % tested positive after C&amp;D), while feed trucks were the least likely contaminated (8.19 % of tires testing positive before C&amp;D and 15.12 % testing positive after C&amp;D). Based on PEDV RNA detection, we demonstrated that quaternary ammonium and glutaraldehyde is a more effective disinfectant compared to advanced hydrogen peroxide in eliminating detectable PEDV RNA. Results also show that truck cabins are just as contaminated as the exterior of their vehicles. Based on these results, vehicle biosecurity measures should be evaluated and modified to prevent the spread of PEDV.</div></div>","PeriodicalId":20413,"journal":{"name":"Preventive veterinary medicine","volume":"237 ","pages":"Article 106447"},"PeriodicalIF":2.2,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143203153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}