Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_3-s36-1
Ito Minako, R. Kaneko
In neurodegenerative diseases such as Alzheimer's disease (AD), the appearance and accumulation of aggregates of Amyloid beta (Aβ) and phosphorylated Tau is triggered by some stimulus such as an immune response. Inflammatory bowel disease was also reported to be a risk factor for dementia in humans, but the mechanism remains unclear. The aim of this study is to develop novel therapeutic strategies by clarifying the immune responses that regulate the expansion of cells expressing Aβ and phosphorylated Tau. We found that colitis enhanced the pathogenesis of AD, and immune cells were infiltrated in the brains and dura maters of mice models of AD by using scRNAseq, suggesting the involvement of immune responses in the development of neurological diseases. Symposium 36
{"title":"Involvement in the development of Alzheimer's disease through activation of systemic immune response.","authors":"Ito Minako, R. Kaneko","doi":"10.1254/jpssuppl.95.0_3-s36-1","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_3-s36-1","url":null,"abstract":"In neurodegenerative diseases such as Alzheimer's disease (AD), the appearance and accumulation of aggregates of Amyloid beta (Aβ) and phosphorylated Tau is triggered by some stimulus such as an immune response. Inflammatory bowel disease was also reported to be a risk factor for dementia in humans, but the mechanism remains unclear. The aim of this study is to develop novel therapeutic strategies by clarifying the immune responses that regulate the expansion of cells expressing Aβ and phosphorylated Tau. We found that colitis enhanced the pathogenesis of AD, and immune cells were infiltrated in the brains and dura maters of mice models of AD by using scRNAseq, suggesting the involvement of immune responses in the development of neurological diseases. Symposium 36","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76800858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.96.0_3-b-p-230
S. Dohgu, Miki Yokoya, F. Takata, T. Iwao, Junichi Matsumoto, Kazunori Sano
{"title":"Characteristics of α-synuclein uptake at the blood-brain barrier","authors":"S. Dohgu, Miki Yokoya, F. Takata, T. Iwao, Junichi Matsumoto, Kazunori Sano","doi":"10.1254/jpssuppl.96.0_3-b-p-230","DOIUrl":"https://doi.org/10.1254/jpssuppl.96.0_3-b-p-230","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82152156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_1-ss-67
Shinichiro Aiba, H. Okamoto, A. Tomita, T. Kusakizako, O. Nureki
{"title":"Structural analysis of the ABC transporter which transports long chain fatty acid CoA.","authors":"Shinichiro Aiba, H. Okamoto, A. Tomita, T. Kusakizako, O. Nureki","doi":"10.1254/jpssuppl.95.0_1-ss-67","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_1-ss-67","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81402271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.96.0_2-b-ss02-6
Qianli Zhang, Akitoshi Miyamoto, T. Arimori, J. Takagi, Naoki Watanabe
{"title":"Development of fast-dissociating recombinant antibody probes for multiplexed super-resolution molecular mapping","authors":"Qianli Zhang, Akitoshi Miyamoto, T. Arimori, J. Takagi, Naoki Watanabe","doi":"10.1254/jpssuppl.96.0_2-b-ss02-6","DOIUrl":"https://doi.org/10.1254/jpssuppl.96.0_2-b-ss02-6","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81772643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.96.0_4-b-s40-2
Y. Mori, Akito Nakao, Kenneth Liu, N. Takahashi
{"title":"TRPA channel plays ubiquitous roles in O2 sensing","authors":"Y. Mori, Akito Nakao, Kenneth Liu, N. Takahashi","doi":"10.1254/jpssuppl.96.0_4-b-s40-2","DOIUrl":"https://doi.org/10.1254/jpssuppl.96.0_4-b-s40-2","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81784841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_2-p-180
Uchida Kazuhiko, Nobuya Sakai, K. Shibata
{"title":"Identification of weak-interacting proteins with Sav1 using BioID2 method","authors":"Uchida Kazuhiko, Nobuya Sakai, K. Shibata","doi":"10.1254/jpssuppl.95.0_2-p-180","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_2-p-180","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82322103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.96.0_3-b-p-196
T. Kondo, Erika Sugisawa, Kenta Maruyama
{"title":"Prevention of endotoxic death by a nociceptor-derived anti-microbial peptide.","authors":"T. Kondo, Erika Sugisawa, Kenta Maruyama","doi":"10.1254/jpssuppl.96.0_3-b-p-196","DOIUrl":"https://doi.org/10.1254/jpssuppl.96.0_3-b-p-196","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82496963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.96.0_4-b-p-274
Mihoka Kojima, Daiki Asano, Akane Morita, T. Kashihara, T. Nakahara
{"title":"The interaction between MMP-9 and TNF-α contributes to the exacerbation of capillary degeneration in a rat model of NMDA-induced retinal injury","authors":"Mihoka Kojima, Daiki Asano, Akane Morita, T. Kashihara, T. Nakahara","doi":"10.1254/jpssuppl.96.0_4-b-p-274","DOIUrl":"https://doi.org/10.1254/jpssuppl.96.0_4-b-p-274","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78870645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.96.0_1-b-s02-1
Hideyuki Hakui
{"title":"Identification of a novel causative gene for dilated cardiomyopathy requiring heart transplantation and elucidation of the mechanism of protein homeostasis in cardiomyocytes","authors":"Hideyuki Hakui","doi":"10.1254/jpssuppl.96.0_1-b-s02-1","DOIUrl":"https://doi.org/10.1254/jpssuppl.96.0_1-b-s02-1","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76327135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_3-p-204
Yasuharu Shinoda, Yudai Haga, Takuya Sasaki, K. Fukunaga
Sigma-1 receptor (σ1R) is a chaperone protein localized in ER membrane. Intrinsic molecules like cholesterol and some clinical drugs are known to bind and regulate the receptor. In this decade, twelve mutations of σ1R were discovered in motor neuron diseases like ALS and dHMN. We previously reported that the mutant (E102Q) which was identified in ALS shows abnormal resistant to detergents, intracellular aggregation, and toxicity in NSC-34 cells, a motor neuron-like cultured cells. However, it is fully unknown how the mutation alters the feature of σ1R. We transfected the cells with wildtype and the mutant and performed pulldown assay using cholesterol-beads. Cells were treated with the agonist SA4503 to investigate if the agonist changes the formation of σ1R and lipid complex. Moreover, cells were treated with BODIPY-Cholesterol to analyze cellular distribution of the lipid. As a result, the mutant but not wildtype σ1R binds with cholesterol in NSC-34 cells. SA4503 inhibited the binding of the mutant and cholesterol. BODIPY-Cholesterol was accumulated in the mutant aggregation. These results suggest that ALS-related mutation causes σ1R to bind with cholesterol, leading to aberrant aggregations and toxicity. Moreover, modulations by the agonist can inhibit the binding with the lipid and aggregation. Poster Session
{"title":"The effect of ALS-related mutation on lipid binding of sigma-1 receptor and its relationship with agonist action","authors":"Yasuharu Shinoda, Yudai Haga, Takuya Sasaki, K. Fukunaga","doi":"10.1254/jpssuppl.95.0_3-p-204","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_3-p-204","url":null,"abstract":"Sigma-1 receptor (σ1R) is a chaperone protein localized in ER membrane. Intrinsic molecules like cholesterol and some clinical drugs are known to bind and regulate the receptor. In this decade, twelve mutations of σ1R were discovered in motor neuron diseases like ALS and dHMN. We previously reported that the mutant (E102Q) which was identified in ALS shows abnormal resistant to detergents, intracellular aggregation, and toxicity in NSC-34 cells, a motor neuron-like cultured cells. However, it is fully unknown how the mutation alters the feature of σ1R. We transfected the cells with wildtype and the mutant and performed pulldown assay using cholesterol-beads. Cells were treated with the agonist SA4503 to investigate if the agonist changes the formation of σ1R and lipid complex. Moreover, cells were treated with BODIPY-Cholesterol to analyze cellular distribution of the lipid. As a result, the mutant but not wildtype σ1R binds with cholesterol in NSC-34 cells. SA4503 inhibited the binding of the mutant and cholesterol. BODIPY-Cholesterol was accumulated in the mutant aggregation. These results suggest that ALS-related mutation causes σ1R to bind with cholesterol, leading to aberrant aggregations and toxicity. Moreover, modulations by the agonist can inhibit the binding with the lipid and aggregation. Poster Session","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76425888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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