Pub Date : 2019-11-01DOI: 10.2991/isils-19.2019.45
Yulia N. Kurkina, A. Sirotin, K. Boyarshin, I. Batlutskaya
The effect of antibiotics and vitamin-mineral supplements on Saccharomyces cerevisiae strains CNCM I-1077 (feed additive Lewisel SC Titan Plus for cattle, horses, goats, and sheep) and CNCM I-1079 (feed additive Lewisel SB Titan Plus for poultry, pigs) studied. The yeast contained in the feed additive sown on nutrient medium No. 2 (Saburo agar) with the addition of chloramphenicol. The number of yeast colonies grown with the addition of antibiotics Enroflon, Florfenicol, Intecol, Klindaspectin, Komplicid, Kvinocyclinum, Macradox 200, Pulmokit, Soladoxy 500, Solamox, Spelink-44, Spelink-660, Sulteprim, Tilmipul, Tilmozin and vitamin-mineral complexes Ciprogen, Floram, Lybecrim, Productive Acid, Productive Forte, Rumisol was recorded VitAmMin. The control happened medium without the addition of veterinary drugs. Veterinary drug solutions added to the nutrient medium at concentrations recommended by the manufacturer for use. It found that the number of colonies of S. cerevisiae in the Petri dishes did not change Klindaspectin, Soladoxy 500, Solamox, Spelink-44, Sulteprim, Tilmipul and the acidifier Komplicid; Florfenicol and Kvinocyclinum increased the number of yeast colonies by 14%, and Tilmipul and Lybecrim by 27%. Keywords—yeast, Saccharomyces cerevisiae, antibiotics, vitamin-mineral supplements, feed additive, effect of supplements on yeast strains
{"title":"Effect of Antibiotics and Vitamin-Mineral Supplements on the Saccharomyces Cerevisia Strains of Feed Additives for Poultry and Cattle","authors":"Yulia N. Kurkina, A. Sirotin, K. Boyarshin, I. Batlutskaya","doi":"10.2991/isils-19.2019.45","DOIUrl":"https://doi.org/10.2991/isils-19.2019.45","url":null,"abstract":"The effect of antibiotics and vitamin-mineral supplements on Saccharomyces cerevisiae strains CNCM I-1077 (feed additive Lewisel SC Titan Plus for cattle, horses, goats, and sheep) and CNCM I-1079 (feed additive Lewisel SB Titan Plus for poultry, pigs) studied. The yeast contained in the feed additive sown on nutrient medium No. 2 (Saburo agar) with the addition of chloramphenicol. The number of yeast colonies grown with the addition of antibiotics Enroflon, Florfenicol, Intecol, Klindaspectin, Komplicid, Kvinocyclinum, Macradox 200, Pulmokit, Soladoxy 500, Solamox, Spelink-44, Spelink-660, Sulteprim, Tilmipul, Tilmozin and vitamin-mineral complexes Ciprogen, Floram, Lybecrim, Productive Acid, Productive Forte, Rumisol was recorded VitAmMin. The control happened medium without the addition of veterinary drugs. Veterinary drug solutions added to the nutrient medium at concentrations recommended by the manufacturer for use. It found that the number of colonies of S. cerevisiae in the Petri dishes did not change Klindaspectin, Soladoxy 500, Solamox, Spelink-44, Sulteprim, Tilmipul and the acidifier Komplicid; Florfenicol and Kvinocyclinum increased the number of yeast colonies by 14%, and Tilmipul and Lybecrim by 27%. Keywords—yeast, Saccharomyces cerevisiae, antibiotics, vitamin-mineral supplements, feed additive, effect of supplements on yeast strains","PeriodicalId":20489,"journal":{"name":"Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75398313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.2991/isils-19.2019.18
Andrey Bursakov, N. Abdrazakova, E. Kryazheva, O. Knysh, E. Vaseva, I. Glukharev
During the drugs production, one of the most important requirements of good manufacturing practice (GMP) is the equipment cleaning from active pharmaceutical substance residues, which has to be carried out to prevent cross-contamination in case of switching from the production of one drug to another. The use of a sufficiently sensitive, rapid and simple UV spectrophotomery technique for determining trace amounts of repaglinide, carried out to control the quality of cleaning and cleaning validation in pharmaceutical production, is described. This technique is validated for specificity, linearity, detection limit and quantification limit. The calibration plot is linear in the concentration range of 0.1420 0.2130 mg/ml. The detection limit is 9.87·10, the limit of quantification is 2.96·10. Keywords—validation, UV spectrophotometry, validation of analytical methods, pharmaceutical equipment cleaning, residual amount determination, repaglinide
{"title":"Validation of the analytical method for Repaglinide residual amount determination on the surfaces of cleanrooms and pharmaceutical equipment by means of UV spectrophotometry","authors":"Andrey Bursakov, N. Abdrazakova, E. Kryazheva, O. Knysh, E. Vaseva, I. Glukharev","doi":"10.2991/isils-19.2019.18","DOIUrl":"https://doi.org/10.2991/isils-19.2019.18","url":null,"abstract":"During the drugs production, one of the most important requirements of good manufacturing practice (GMP) is the equipment cleaning from active pharmaceutical substance residues, which has to be carried out to prevent cross-contamination in case of switching from the production of one drug to another. The use of a sufficiently sensitive, rapid and simple UV spectrophotomery technique for determining trace amounts of repaglinide, carried out to control the quality of cleaning and cleaning validation in pharmaceutical production, is described. This technique is validated for specificity, linearity, detection limit and quantification limit. The calibration plot is linear in the concentration range of 0.1420 0.2130 mg/ml. The detection limit is 9.87·10, the limit of quantification is 2.96·10. Keywords—validation, UV spectrophotometry, validation of analytical methods, pharmaceutical equipment cleaning, residual amount determination, repaglinide","PeriodicalId":20489,"journal":{"name":"Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)","volume":"98 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80264353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Early blight (Alternaria solani) is a potential disease of tomato that reduces its production globally both in conventional and tunnel cultivations. Due to variability in pathogenic isolates, prolonged active disease cycle phase and broad host range early blight is very difficult to manage. 8 microorganisms as a bioagent exhibiting inhibitory affects against Alternaria solani, were screened for their activity towards A. solani by a dual culture in vitro assay and in vivo (whole plant) test in vitro studies indicated that the microorganism’s strains strongly inhibited the mycelial growth of the pathogen. The effect of microorganism’s strains on the mycelial growth (mm) of the pathogen proved to be highest with Trichoderma sp. (0.55) followed by Pseudomonas brassicacearum (0.74) and Pseudomonas jessenii (0.81) on the high concentration (106 cells ml-1) compared to the control (2.30). On the other hand, Bacillus mycoides (2.14) in vivo studies 9 microorganism’s strains were applied in two different application (foliar – soil) and two different varieties (Labella – Romano). The results showed significant reductions in the disease severity (%) with the treatment by Trichoderma sp (2%) followed by Bacillus thuringiensis (3%) and Bacillus mycoides (5%) compared with the control (46%) of Romano variety, while there were a less significant reductions in the disease severity (%) with the treatments compared with the control (16%) of Labella variety . The efficacy of antagonists to suppress the early blight disease varied in respect to the time and type of application. Keywords—Altrnaria solani, biocontrol, Eearly blight, microorganisms, potato
{"title":"Biological Control of Early Blight on Potato Caused by Alternaria Solani by Some Bioagents","authors":"A. Aldiba, Ivan Dmitrievich Escov","doi":"10.2991/isils-19.2019.1","DOIUrl":"https://doi.org/10.2991/isils-19.2019.1","url":null,"abstract":"Early blight (Alternaria solani) is a potential disease of tomato that reduces its production globally both in conventional and tunnel cultivations. Due to variability in pathogenic isolates, prolonged active disease cycle phase and broad host range early blight is very difficult to manage. 8 microorganisms as a bioagent exhibiting inhibitory affects against Alternaria solani, were screened for their activity towards A. solani by a dual culture in vitro assay and in vivo (whole plant) test in vitro studies indicated that the microorganism’s strains strongly inhibited the mycelial growth of the pathogen. The effect of microorganism’s strains on the mycelial growth (mm) of the pathogen proved to be highest with Trichoderma sp. (0.55) followed by Pseudomonas brassicacearum (0.74) and Pseudomonas jessenii (0.81) on the high concentration (106 cells ml-1) compared to the control (2.30). On the other hand, Bacillus mycoides (2.14) in vivo studies 9 microorganism’s strains were applied in two different application (foliar – soil) and two different varieties (Labella – Romano). The results showed significant reductions in the disease severity (%) with the treatment by Trichoderma sp (2%) followed by Bacillus thuringiensis (3%) and Bacillus mycoides (5%) compared with the control (46%) of Romano variety, while there were a less significant reductions in the disease severity (%) with the treatments compared with the control (16%) of Labella variety . The efficacy of antagonists to suppress the early blight disease varied in respect to the time and type of application. Keywords—Altrnaria solani, biocontrol, Eearly blight, microorganisms, potato","PeriodicalId":20489,"journal":{"name":"Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)","volume":"375 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80558169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.2991/isils-19.2019.66
O. Schuklina, Ekaterina Enzekrei, S. Zavgorodniy, O. Ermolenko, I. Voronchihina, Fedor Klimenkov, I. Klimenkova, Alexandr Polkhovskiy
—The article presents the results of breeding in the department of remote hybridization of the Main Botanical Garden n.a. N.V. Tsitsin (MBG RAS) from 1946 to 2019. The aim of this work is to create high-yielding varieties of spring wheat of an intensive type that most fully meet the peculiar conditions in a changing climate. During this time, next varieties were created and passed the State variety test: Hybrid 56, Vostok, Grekum 114, Botanicheskaya 2, Botanicheskaya 3, Botanicheskaya 4, Botanicheskaya 5 and Botanicheskaya 81.
{"title":"Retrospective of Spring Wheat-couch Hybrids Breeding in the Department of Remote Hybridization MBG RAS","authors":"O. Schuklina, Ekaterina Enzekrei, S. Zavgorodniy, O. Ermolenko, I. Voronchihina, Fedor Klimenkov, I. Klimenkova, Alexandr Polkhovskiy","doi":"10.2991/isils-19.2019.66","DOIUrl":"https://doi.org/10.2991/isils-19.2019.66","url":null,"abstract":"—The article presents the results of breeding in the department of remote hybridization of the Main Botanical Garden n.a. N.V. Tsitsin (MBG RAS) from 1946 to 2019. The aim of this work is to create high-yielding varieties of spring wheat of an intensive type that most fully meet the peculiar conditions in a changing climate. During this time, next varieties were created and passed the State variety test: Hybrid 56, Vostok, Grekum 114, Botanicheskaya 2, Botanicheskaya 3, Botanicheskaya 4, Botanicheskaya 5 and Botanicheskaya 81.","PeriodicalId":20489,"journal":{"name":"Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85275618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.2991/isils-19.2019.79
V. Strizhevskaya, V. Salautin, I. Simakova, E. Volf, Maksim Maradudin
The aim of this paper was to study the influence on morpho-functional indicators of the organism of experimental animals by regular consumption of jelly (kissel) concentrates in industrial production in comparison with the jelly (kissel) prepared according to the set of technical standards. The impact evaluation of jelly (kissel) concentrates was carried out using biological methods in animal experiments using morphological research of organs and tissues obtained after dissection of animals of experimental and control groups. The results of the study showed that regular consumption of jelly (kissel) from concentrates may have an adverse effect on the body. This fact must be considered when formulating rations, and the preparation of cold sweet dishes according to the traditional technology is preferred. Keywords—jelly (kissel) concentrates, in vivo experiments, influence on the body
{"title":"Safety study of jelly (kissel) concentrates in the in vivo experiments","authors":"V. Strizhevskaya, V. Salautin, I. Simakova, E. Volf, Maksim Maradudin","doi":"10.2991/isils-19.2019.79","DOIUrl":"https://doi.org/10.2991/isils-19.2019.79","url":null,"abstract":"The aim of this paper was to study the influence on morpho-functional indicators of the organism of experimental animals by regular consumption of jelly (kissel) concentrates in industrial production in comparison with the jelly (kissel) prepared according to the set of technical standards. The impact evaluation of jelly (kissel) concentrates was carried out using biological methods in animal experiments using morphological research of organs and tissues obtained after dissection of animals of experimental and control groups. The results of the study showed that regular consumption of jelly (kissel) from concentrates may have an adverse effect on the body. This fact must be considered when formulating rations, and the preparation of cold sweet dishes according to the traditional technology is preferred. Keywords—jelly (kissel) concentrates, in vivo experiments, influence on the body","PeriodicalId":20489,"journal":{"name":"Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)","volume":"508 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77003270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.2991/isils-19.2019.33
O. Khaeva, B. Tsugkiev, L. Ikoeva
Bacteria of the genus Propionibacteruim are the microorganisms with great biotechnological potential. The article deals with studies of biological properties of new propionic acid bacteria strains isolated from raw milk in different regions of Republic of North Ossetia-Alania. According to morphological characteristics, the cells of the isolated strains are motionless sticks with rounded ends that do not form spores and are gram-positive. The ability of strains to ferment carbohydrates in biochemical tests was determined. The research results showed that the studied bacterial strains were referred to Propionibacterium freudenreichii. Resistance of microorganism strains to low pH values and gastrointestinal tract secretions was studied. Keywords—propionic acid bacteria; genus Propionibacterium; Propionibacterium freudenreichii; identification; acid resistance; bile resistance
{"title":"Isolation and Selection of Propionic Acid Bacteria Promising for Biotechnological Production","authors":"O. Khaeva, B. Tsugkiev, L. Ikoeva","doi":"10.2991/isils-19.2019.33","DOIUrl":"https://doi.org/10.2991/isils-19.2019.33","url":null,"abstract":"Bacteria of the genus Propionibacteruim are the microorganisms with great biotechnological potential. The article deals with studies of biological properties of new propionic acid bacteria strains isolated from raw milk in different regions of Republic of North Ossetia-Alania. According to morphological characteristics, the cells of the isolated strains are motionless sticks with rounded ends that do not form spores and are gram-positive. The ability of strains to ferment carbohydrates in biochemical tests was determined. The research results showed that the studied bacterial strains were referred to Propionibacterium freudenreichii. Resistance of microorganism strains to low pH values and gastrointestinal tract secretions was studied. Keywords—propionic acid bacteria; genus Propionibacterium; Propionibacterium freudenreichii; identification; acid resistance; bile resistance","PeriodicalId":20489,"journal":{"name":"Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80507498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.2991/isils-19.2019.42
A. Kovrigin, G. Pokhodnya, N. Shvetsov, V. Kotarev
The automatic adaptive feeder for fish feeding in recirculation plants has been developed and tested. It was found that the use of this feeder allowed to increase the yield of fry per 1 m3 by 35% and 13.6%; the yield of fingerling – by 45.9% and 18.5%; in the final period of cultivation – by 52.3% and 21.9% in comparison with hand-feeding and traditional auto-feeding without the adaptation function of the autofeeder. Accordingly, using an automatic adaptive feeder, it was possible to obtain by 71.7 kg and 37.6 kg more fish per 1 m3 of recirculating aquaculture system (RAS) during one production cycle than using the existing technology. It helps to make an additional clear profit from the sale of products for 6,702 rubles 88 kopecks from 1 m3 of RAS in comparison with handfeeding and 3,503 rubles 09 kopecks in comparison with automatic feeding without the adaptation system. Keywords—recycling plants for fish farming, RAS, fish automatic feeding, automatic feeder for fish, feeding behavior of fish, sharp-tooth catfish
{"title":"Productivity of Sharp-Tooth Catfish Depending on Feeding Methods","authors":"A. Kovrigin, G. Pokhodnya, N. Shvetsov, V. Kotarev","doi":"10.2991/isils-19.2019.42","DOIUrl":"https://doi.org/10.2991/isils-19.2019.42","url":null,"abstract":"The automatic adaptive feeder for fish feeding in recirculation plants has been developed and tested. It was found that the use of this feeder allowed to increase the yield of fry per 1 m3 by 35% and 13.6%; the yield of fingerling – by 45.9% and 18.5%; in the final period of cultivation – by 52.3% and 21.9% in comparison with hand-feeding and traditional auto-feeding without the adaptation function of the autofeeder. Accordingly, using an automatic adaptive feeder, it was possible to obtain by 71.7 kg and 37.6 kg more fish per 1 m3 of recirculating aquaculture system (RAS) during one production cycle than using the existing technology. It helps to make an additional clear profit from the sale of products for 6,702 rubles 88 kopecks from 1 m3 of RAS in comparison with handfeeding and 3,503 rubles 09 kopecks in comparison with automatic feeding without the adaptation system. Keywords—recycling plants for fish farming, RAS, fish automatic feeding, automatic feeder for fish, feeding behavior of fish, sharp-tooth catfish","PeriodicalId":20489,"journal":{"name":"Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79947474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.2991/isils-19.2019.12
N. Boyko, E. Zhilyakova, O. Novikov, D. Pisarev, A. Bondarev, D. Naplekov, D. Martceva
Aim of this study is the theoretical justification and experimental confirmation of new approach to description of equilibrium state in extraction system vegetal raw materialextractant. In framework of this study the milled raw material was used containing the particles sized 0,1-0,5 mm: roots and rhyzomes of Glycyrrhiza glabra or uralensis, leaves of Uvaurzi, leaves of Eucalyptus viminalis, flowers of Helichrysum arenarium, flowers of Calendula officinalis and etc. The process of equilibrium establishment was studied at temperature levels such as 4, 20, 40 and 60±1oС, wherein the method of simple maceration was implemented for 24 hours of decoction. Interphase distribution of BAS was studied using the proportion of raw material weight / extractant volume as 1:5, 1:10, 1:20, 1:40. The watery ethanol solution of 70 or 80±1 % vol. was chosen as an extractant for the purpose of this study. Assay test of BAS in raw material was carried out with the help of RP HPLC instrumentation method. Experimental points for all the studied BAS from various types of vegetal raw materials are well-approximated by regression lines built up in theoretically predicted coordinates, which is confirmed by the high value of determination coefficient R2≥0.99. The new approach has been proposed to description of equilibrium state in extraction system vegetal raw material-extractant, which is based on the promotion of hypothesis, according to which the mechanism of interphase molecular distribution of BAS in extraction system is explained and described by classic Boltzmann distribution for discrete values of molecular energy (or Fermi-Dirac quantum distribution). The developed and experimentally confirmed mathematical model was based on the promoted hypothesis, and it successfully described the relation of the main parameters of extraction system, which allows to develop the rational technology of tincture or extracts manufacturing. Keywords—extraction, vegetal raw material, biologically active substances, equilibrium, Boltzmann distribution for discrete values of molecular energy, Fermi-Dirac quantum distribution.
{"title":"A New Approach To Description Of Equilibrium State In The Extraction System Of Vegetal Raw Material-Extractant","authors":"N. Boyko, E. Zhilyakova, O. Novikov, D. Pisarev, A. Bondarev, D. Naplekov, D. Martceva","doi":"10.2991/isils-19.2019.12","DOIUrl":"https://doi.org/10.2991/isils-19.2019.12","url":null,"abstract":"Aim of this study is the theoretical justification and experimental confirmation of new approach to description of equilibrium state in extraction system vegetal raw materialextractant. In framework of this study the milled raw material was used containing the particles sized 0,1-0,5 mm: roots and rhyzomes of Glycyrrhiza glabra or uralensis, leaves of Uvaurzi, leaves of Eucalyptus viminalis, flowers of Helichrysum arenarium, flowers of Calendula officinalis and etc. The process of equilibrium establishment was studied at temperature levels such as 4, 20, 40 and 60±1oС, wherein the method of simple maceration was implemented for 24 hours of decoction. Interphase distribution of BAS was studied using the proportion of raw material weight / extractant volume as 1:5, 1:10, 1:20, 1:40. The watery ethanol solution of 70 or 80±1 % vol. was chosen as an extractant for the purpose of this study. Assay test of BAS in raw material was carried out with the help of RP HPLC instrumentation method. Experimental points for all the studied BAS from various types of vegetal raw materials are well-approximated by regression lines built up in theoretically predicted coordinates, which is confirmed by the high value of determination coefficient R2≥0.99. The new approach has been proposed to description of equilibrium state in extraction system vegetal raw material-extractant, which is based on the promotion of hypothesis, according to which the mechanism of interphase molecular distribution of BAS in extraction system is explained and described by classic Boltzmann distribution for discrete values of molecular energy (or Fermi-Dirac quantum distribution). The developed and experimentally confirmed mathematical model was based on the promoted hypothesis, and it successfully described the relation of the main parameters of extraction system, which allows to develop the rational technology of tincture or extracts manufacturing. Keywords—extraction, vegetal raw material, biologically active substances, equilibrium, Boltzmann distribution for discrete values of molecular energy, Fermi-Dirac quantum distribution.","PeriodicalId":20489,"journal":{"name":"Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86279243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the work was to diagnose diseases through autopsy and additional examinations and to determine the mortality structure and statistics in pigs of fattening groups on pig farms of industrial type. The objects of the study were 626 pigs from 15 pig «farrow-to-finish» farms in a number of regions of the European part of Russia. All animals were autopsied by the authors together with farm specialists in 20092019. Autopsy results in many cases are supplemented by the results of histological, bacteriological studies, ELISA studies, PCR. Over the entire period of the study of pigs in the fattening groups, streptococcosis, hypovitaminosis E mycotoxicosis and actinobacillosis pleuropneumonia were most commonly diagnosed; intestinal inversion, enzootic pneumonia and gastric ulcer were somewhat less often. The absence of the viral causes of death, apart from the isolated cases of circovirus is indicative. When comparing the results of the study in the periods 2009-2015 and 2016-2019, attention is drawn to the increase in the frequency of cases of streptococcosis, intestinal inversion, cystitis and a sharp decrease in the frequency of cases of actinobacillosis pleuropneumonia. Changing indicators of the structure and mortality statistics for a number of years indicate the feasibility of pathological monitoring on pig farms, bearing in mind the constantly changing situation in diseases, depending on numerous factors affecting the number of animals. Keywords—pig, statistics of mortality
{"title":"Structure and Statistics of Mortality in Pig Feeding Groups on Industrial Type Farms in 2009-2019","authors":"V. Balabanova, A. Kudriashov","doi":"10.2991/isils-19.2019.4","DOIUrl":"https://doi.org/10.2991/isils-19.2019.4","url":null,"abstract":"The aim of the work was to diagnose diseases through autopsy and additional examinations and to determine the mortality structure and statistics in pigs of fattening groups on pig farms of industrial type. The objects of the study were 626 pigs from 15 pig «farrow-to-finish» farms in a number of regions of the European part of Russia. All animals were autopsied by the authors together with farm specialists in 20092019. Autopsy results in many cases are supplemented by the results of histological, bacteriological studies, ELISA studies, PCR. Over the entire period of the study of pigs in the fattening groups, streptococcosis, hypovitaminosis E mycotoxicosis and actinobacillosis pleuropneumonia were most commonly diagnosed; intestinal inversion, enzootic pneumonia and gastric ulcer were somewhat less often. The absence of the viral causes of death, apart from the isolated cases of circovirus is indicative. When comparing the results of the study in the periods 2009-2015 and 2016-2019, attention is drawn to the increase in the frequency of cases of streptococcosis, intestinal inversion, cystitis and a sharp decrease in the frequency of cases of actinobacillosis pleuropneumonia. Changing indicators of the structure and mortality statistics for a number of years indicate the feasibility of pathological monitoring on pig farms, bearing in mind the constantly changing situation in diseases, depending on numerous factors affecting the number of animals. Keywords—pig, statistics of mortality","PeriodicalId":20489,"journal":{"name":"Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84377406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.2991/isils-19.2019.49
E. Maslova, Tatyana Glodik, Valeria Semykina, Natalya Gulya, T. Perelygina
—In this paper, we studied the antimicrobial activity of callus tissue Salvia pratensis L. obtained in vitro against Escherichia coli and Staphylococcus aureus . The most effective sterilizing agents for disinfection of plant explants with their introduction into in vitro culture were selected. The composition of the nutrient medium for the cultivation of callus tissues of S. pratensis has been optimized. A comparative analysis for the antibacterial activity of plant extracts obtained from callus tissues and intact plants was carried out, and as a result it was found that extracts from callus tissue demonstrate the most pronounced antibacterial properties in concentrations of 100% and at a dilution of 1:10, in contrast to extracts from leaves and flowers of an intact plant.
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