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Mechanisms of anti-stress action of low-intensive mm radiation on microcirculation of animals under stress conditions 低强度mm辐射对应激条件下动物微循环的抗应激作用机制
M. Ravaeva, Anastasia Furkovskaya, E. Chuyan, E. Birukova, Olga Kornilenko
— animal studies with laser Doppler flowmetry showed, that there were local and systemic changes in the microcirculation caused by hypokinetic stress, what allowed us to expand our understanding of the pathophysiological processes caused by the influence of a stressing factor on the body. A study of the isolated EHF influence on the microcirculation and its combination with stress-modeling helped us to establish and scientifically substantiate the possibility of using extremely high frequency electromagnetic radiation for correction body disorders caused by stress.
-用激光多普勒血流仪进行的动物研究表明,低运动应激引起的微循环存在局部和全身变化,这使我们能够扩大对应激因素对身体影响所引起的病理生理过程的理解。孤立的EHF对微循环的影响及其与应力模拟相结合的研究帮助我们建立并科学地证实了利用极高频率电磁辐射校正应力引起的身体紊乱的可能性。
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引用次数: 0
New Ingredient in Bakery, Technological and Nutritional Effects of Buttermilk 烘焙新原料、工艺及酪乳的营养作用
M. Ognean, C. Ognean, Simona-Ştefania Hogea
—Buttermilk is a by-product obtained during butter preparation, has a nutritional composition similar to skimmed milk but with an increased proportion of phospholipids. Phospholipids was proved to have functional properties. Buttermilk is produced in large amounts due the preparation of butter and could be considered for use in various food product to obtained functional, sensorial and nutritional improved product. To increase the sustainability we used fresh, unprocessed buttermilk. The aim of this study was to investigate the effects of water replacement in bread formulation with fresh, unpasteurized buttermilk. Dough rheology was improved especially at 50% buttermilk addition and at higher levels did not put on advantage. Bread with added buttermilk showed small improvement of specific volume; denser crumb; pleasant smell and taste; yellowish crumb; darker crust colour. The nutritional value was improved, especially mineral and protein content and amino acid balance. White bread showed more significant improvement of nutritional value.
-脱脂乳是黄油制备过程中产生的副产品,其营养成分与脱脂乳相似,但磷脂的比例增加。磷脂被证明具有功能特性。由于黄油的制备,酪乳可以大量生产,可以考虑在各种食品中使用,以获得功能,感官和营养的改良产品。为了增加可持续性,我们使用了新鲜的、未经加工的酪乳。本研究的目的是研究用新鲜的、未经巴氏消毒的酪乳代替面包配方中的水的影响。酪乳添加量为50%时,面团流变学有明显改善,添加量再高则无明显改善。添加酪乳的面包比容略有改善;密集的碎屑;宜人的气味和味道;黄瓤;外壳颜色较深。营养价值显著提高,尤其是矿物质、蛋白质含量和氨基酸平衡。白面包的营养价值改善更为显著。
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引用次数: 1
Dynamics of the Accumulation of the Biologically Active Substances Sanguisorba Officinalis L. Depending on Vertical Zonal 地榆生物活性物质在垂直地带性上的积累动态
B. Tsugkiev, L. Gagieva, V. Cherniavskih, E. Dumacheva, S. Korolkova
The species Sanguisorba officinalis L. is widespread in the Republic of North Ossetia-Alania and is promising for the study and practical use of the pharmacological and food industry. The aim of the work is to analyze the dynamics of biologically active substances in the phytomass S. officinalis depending on the altitude gradient under conditions of RNO-Alania. Studies were carried out in 2012-2016. Samples of vegetable raw materials were taken at 5 stationary points located in various high-altitude belts: 1079, 1480, 1760, 1810 and 2300 m above the sea level. Studies were conducted using standard methods. The air-dry yield of S. officinalis above-ground phytomass in an averaged between 0.669 to 1.05 kg/m over the years of research. The content of the studied biologically active substances in the above-ground phytomass of S. officinalis was found to change with increasing growth height. At a height of 1079 m, a high content of nitrogen-free extractive substances, hydroxycoric acids (in terms of chlorogenic), leukoantocyans, catechins, tanning substances was found in tissues. At a height of 1480 m, the accumulation of crude protein and routine was observed. At an altitude of 1810 m, fiber, ash elements, ascorbic acid, saponins (in terms of ursolic acid), and carotenes accumulate in grass. At a height of 2300 m, plant tissues show maximum levels of crude fat and flavonoids. The obtained data make it possible to estimate antioxidant and P-vitamin properties of S. officinalis and to recommend collection of vegetable raw materials of S. officinalis at different height to obtain certain biologically active substances. Effective and low-toxic natural polyphenolic antioxidants of S. officinalis can be used in the food, cosmetic and pharmaceutical industries. Keywords—Sanguisorba officinalis L., biological resources Caucasus, biologically active substances, antioxidants
山血草(Sanguisorba officinalis L.)广泛分布于北奥塞梯-阿拉尼亚共和国,在药理学和食品工业的研究和实际应用方面具有广阔的前景。本研究的目的是分析在RNO-Alania条件下,植物中生物活性物质随海拔梯度的变化规律。研究于2012年至2016年进行。在海拔1079、1480、1760、1810和2300 m不同海拔带的5个静止点采集蔬菜原料样品。研究采用标准方法进行。历年研究结果表明,山茱萸地上生物量的风干产量平均在0.669 ~ 1.05 kg/m之间。研究发现,随着生长高度的增加,山茱萸地上生物量中生物活性物质的含量发生了变化。在1079 m的高度,组织中发现了大量的无氮提取物质、羟基酸(以绿原计)、白细胞青素、儿茶素、鞣制物质。在海拔1480 m处,观察了粗蛋白质和常规蛋白的积累。在海拔1810米的地方,纤维、灰分元素、抗坏血酸、皂苷(以熊果酸计算)和胡萝卜素在草中积累。在海拔2300 m处,植物组织中粗脂肪和类黄酮含量最高。所获得的数据可用于估计马蹄草的抗氧化性能和p -维生素性能,并可为不同高度马蹄草蔬菜原料的采集提供建议,以获得一定的生物活性物质。officinalis是一种高效、低毒的天然多酚类抗氧化剂,可用于食品、化妆品和制药等行业。关键词:地榆,生物资源高加索,生物活性物质,抗氧化剂
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引用次数: 1
Experimental Models of Study and Prevention of Stress in Birds in Industrial Poultry Farming 工业化家禽养殖中鸟类应激的实验模型研究及预防
T. Pogrebnyak, E. Khorolskaya, S. Chernyavskikh, I. Sagalaeva
—In birds, acute stress (a three-day 12-hour inversion of the photomode) is associated with the dominant activity of the sympathetic hypothalamus and hippocampus, which suppress the tone of the parasympathetic section and the reticular formation of the midbrain. The effect of hyperglycemia on the background of a negative chronotropic effect indicates a mismatch of the functions of the autonomic sections of the hypothalamus on the 30 th day. Chronic stress in birds (30-days crowding) causes persistent pathological stress of the functions of the anterior hypothalamus and reticular formation of the middle brain with the suppression of papasympathetic tone of the posterior hypothalamus and high functional activity of the hippocampus, determining the switching of the ventricles of the heart to a more economical mode of functioning by the 30th day. having a cholinoblocking component in the mechanism of its influence during neurogenic stress and in the post-stress period is excluded, but it is possible to use stressprotektors.
在鸟类中,急性应激(三天12小时的光模倒置)与交感下丘脑和海马体的主要活动有关,这抑制了副交感神经部分的张力和中脑的网状形成。在负变时效应的背景下高血糖的影响表明在第30天下丘脑自主神经部分的功能不匹配。鸟类慢性应激(30天的拥挤)导致下丘脑前部功能和中脑网状结构的持续病理应激,下丘脑后部的pap交感神经张力受到抑制,海马的高功能活动,决定了心脏心室在第30天转向更经济的功能模式。排除了其在神经源性应激和应激后时期的影响机制中具有胆碱阻断成分,但可能使用应激保护因子。
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引用次数: 0
Rhizospheric Bacteria, Destructors of Toxic Aromatic Compounds 根际细菌:有毒芳香族化合物的破坏者
T. Anokhina, T. Esikova, V. Polivtseva, L. Iminova, I. Solyanikova
Bacteria capable of degradation of a number of toxic organic compounds (aromatic hydrocarbons, n-alkanes, phenol and its chlorinated derivatives) were isolated from the rhizosphere of plants growing on “clean” and oil-contaminated soil. The most active strain, Lysinibacillus sp. Fg1 was able to utilize more than 15 individual substrates. Six bacterial strains of the genus Pseudomonas decomposed polycyclic aromatic hydrocarbons, suppressed the growth of phytopathogenic fungi and bacteria, and synthesized phytohormones auxins. Keywords—bacteria-destructors, rhizospheric microorganisms, polycyclic aromatic hydrocarbons, phenol,
从生长在“干净”和受石油污染的土壤上的植物根际中分离出能够降解许多有毒有机化合物(芳烃、正烷烃、苯酚及其氯化衍生物)的细菌。活性最强的菌株Lysinibacillus sp. Fg1能够利用超过15个单独的底物。6株假单胞菌分解多环芳烃,抑制植物致病真菌和细菌的生长,合成植物激素生长素。关键词:细菌破坏者,根际微生物,多环芳烃,苯酚,
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引用次数: 0
Use of biologically active substances in feeding young animals 使用生物活性物质喂养幼畜
N. Ordina, S. Kontsevaya, N. Sorokina, K. Mezinova
An important problem of cattle breeding in Russia is the creation of a solid fodder base. Due to its absence, the genetic potential of dairy and beef cattle in our country is realized only by 30-50%. This work is devoted to the study of the influence of the belotine feed additive on the productivity of meat young animals. The studies have shown that in the case of the addition of 10% of the dry matter requirement, experimental groups surpass the control groups. Keywords—young animals, feeding, feed additives, biologically active substances, mixed fodder – starters, protein belotine
俄罗斯养牛的一个重要问题是建立一个坚实的饲料基地。由于它的缺失,我国乳牛和肉牛的遗传潜力只实现了30-50%。本工作致力于研究beltine饲料添加剂对肉用幼畜生产能力的影响。研究表明,在添加10%干物质需要量的情况下,试验组的干物质需要量超过对照组。关键词:幼龄动物;饲料;饲料添加剂;生物活性物质
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引用次数: 2
Taxonomical Structure of Black Soil Bacterial Community on the Level of Phyla 黑土细菌群落在门水平上的分类结构
A. Nechayeva, V. Iatsenko, K. Boyarshin, V. Klyueva, O. Bespalova, Yuliya Kurkina, I. Batlutskaya
—Soil microbial communities perform a number of important functions ensuring fertility. They depend on physical and chemical composition of soil and applied agricultural technology. To control the state of the soil, it is necessary to use methods that allow to quickly assess the dynamics of the structure of microbial communities. To develop convenient method for analysis of microbiota the set of taxon-specific primers for RT PCR, recognizing 16S rRNA genes of domain Bacteria , six bacterial phyla and one class was chosen. Calculation of the percentages of lower-order taxa in the upper-order taxon was carried out based on the number of amplification cycles required to achieve the threshold value of fluorescence intensity taking into account the values of amplification factors. Taxonomic structure of the bacterial component of the soil microbial community was analyzed using as an example a sample of the surface layer of arable black soil enriched with regular application of organic fertilizers. The compiled analysis protocol made it possible to obtain data on the percentages of phyla Firmicutes, Bacteroidetes, Actinobacteria, Verrucomicrobia , and of class Gammaproteobacteria belonging to phylum Proteobacteria
-土壤微生物群落在确保肥力方面发挥着许多重要作用。它们取决于土壤的物理和化学成分以及应用的农业技术。为了控制土壤的状态,有必要使用能够快速评估微生物群落结构动态的方法。为了建立简便的微生物区系分析方法,我们选择了一套分类特异的RT - PCR引物,用于识别域细菌(domain Bacteria)的16S rRNA基因。根据达到荧光强度阈值所需的扩增周期数,考虑扩增因子的值,计算低阶分类群在高阶分类群中的百分比。以经常施用有机肥的黑土耕层土壤为例,分析了土壤微生物群落细菌组成的分类结构。编制的分析方案可以获得厚壁菌门、拟杆菌门、放线菌门、Verrucomicrobia门和属于变形菌门的γ变形菌门的百分比数据
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引用次数: 0
Study and Modeling the Influence of a Solvent on Extraction of Eugenol from Laurus Nobilis L. Leaves 溶剂对月桂叶中丁香酚提取的影响及模拟研究
Valentina Radyukova, N. Boyko, E. Zhilyakova, Z. Tsvetkova, D. Martceva, A. Malyutina, Valentina Kasakova, D. Fadeeva, Ghadeer Balloul
this paper presents the results of a study and modeling of the influence of the solvent’s dielectric constant on the extraction of eugenol from the Laurus nobilis L leaves. The aim of this work is to study the effect of the solvent on the extraction of eugenol from the leaves of Laurus nobilis L., to propose a theoretical model for describing the results. Shredded plant material of Laurus nobilis L. leaves 0.1-0.5 mm. Aqueous solutions of ethanol (23, 39, 55, 73, 80, 96±1% v/v). The extracts were obtained by a ratio of plant materials extractant 1:5 (w/v), insisting for 24 hours at a temperature of 24±1°C. Analysis of the extracts was carried out using reverse phase high performance liquid chromatography and a standard sample of eugenol. The dependence of the concentration of eugenol in alcohol-water extracts on the concentration of ethanol in solution was studied. It was found that the maximum concentration of eugenol is observed in solutions with ethanol concentrations of 85±15 % vol. This range of ethanol concentrations corresponds to a dielectric constant of the solvent of 33.5±3.5 units. The regression equation of the dependence of the concentration of eugenol in the extract on the dielectric constant of alcohol-water solutions is obtained. It was found that the dielectric constant of the solvent has the most significant effect on the process of isolating the phytocomponent from plant materials. A mathematical model is theoretically grounded and tested to describe the dependence of the concentration of eugenol on the dielectric constant of water-ethanol solutions. The optimal range of solvent dielectric constant was found to achieve the maximum concentration of eugenol in the extract. Keywords—Laurus nobilis L. leaves, solvents, HPLC analysis, dielectric constant, eugenol
本文对溶剂介电常数对月桂叶中丁香酚提取的影响进行了研究并建立了模型。本研究旨在研究溶剂对月桂叶中丁香酚提取的影响,并提出描述提取结果的理论模型。月桂叶碎料0.1 ~ 0.5 mm。乙醇水溶液(23、39、55、73、80、96±1% v/v)。提取液按植物材料萃取剂1:5 (w/v)的比例,在24±1℃的温度下保存24小时。以丁香酚为标准品,采用反相高效液相色谱法对提取物进行分析。研究了醇水提取物中丁香酚的浓度与乙醇浓度的关系。研究发现,在乙醇浓度为85±15% vol的溶液中,丁香酚的最大浓度。乙醇浓度范围对应于溶剂的介电常数为33.5±3.5单位。得到了萃取液中丁香酚浓度与醇-水溶液介电常数关系的回归方程。研究发现,溶剂的介电常数对从植物材料中分离植物成分的过程影响最大。从理论上建立了一个数学模型,并进行了测试,以描述丁香酚浓度对水-乙醇溶液介电常数的依赖关系。确定了溶剂介电常数的最佳范围,使丁香酚在提取液中的浓度达到最大。关键词:月桂叶,溶剂,HPLC分析,介电常数,丁香酚
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引用次数: 0
Methods of Extraction of Medicinal Vegetable Raw Materials in Phytosubstances Technology 植物物质技术中药用植物原料的提取方法
I. Kaukhova, V. Weinstein, M. Burakova, M. Aroyan, E. Novikova
The article discusses the technology of obtaining phytosubstances using modern methods of extraction of medicinal plant materials: ultrasonic extraction, two-phase extraction. On the example of various types of plant materials, a comparative analysis was carried out and the advantages and disadvantages of the considered extraction methods were
本文论述了现代药用植物物质提取方法:超声提取、两相提取等获得植物物质的技术。以不同类型的植物材料为例,进行了比较分析,分析了所考虑的提取方法的优缺点
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引用次数: 1
Microbial Community of Biogas Plant Feeding with Complex Substrate: Archaea/Bacteria Ratio Dynamics by the Stages of Fermentation 复杂底物饲喂沼气植物的微生物群落:发酵阶段古菌/细菌比例的动态变化
V. Iatsenko, A. Nechayeva, K. Boyarshin, V. Klyueva, Dmitriy Ohrimchuk, V. Bredihin, I. Batlutskaya
The technology of anaerobic fermentation of organic substrates and especially of organic wastes with rich microbiota needs effective method to observe the microbial community on the different stages of substrate transformation, including its digestion, fermentation and conversion of its products to methane. The fast and cheap method based on taxon-specific RT PCR is developed to assess density of Archaea and Bacteria population in complex microbial communities. The data obtained on the samples from the industrial biogas plant on the different technological stages are consistent with processes that should evolve during
有机基质的厌氧发酵技术,特别是微生物群丰富的有机废弃物的厌氧发酵技术,需要有效的方法来观察基质转化不同阶段的微生物群落,包括消化、发酵和产物转化为甲烷。建立了一种快速、廉价的基于分类群特异性RT - PCR的方法来评估复杂微生物群落中古生菌和细菌的种群密度。在不同技术阶段从工业沼气厂获得的样品数据与在此期间应该发展的过程是一致的
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引用次数: 0
期刊
Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)
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