Pub Date : 1982-06-01DOI: 10.3181/00379727-170-41415
J B Marsh, C E Sparks
Abstract Rat livers from animals fed ad lib or fasted 24 hr were perfused by the single pass technique with 3H-amino acids and label incorporation into lipoprotein apoproteins was measured. The lipoproteins were separated into very low density lipoproteins (d < 1.006), low-density lipoproteins (1.006 < d < 1.06), and high-density lipoproteins (1.06 < d < 1.21) by ultracentrifugation and the apoproteins of each density fraction were analyzed by gel filtration column chromatography in the presence of sodium dodecyl sulfate. The apoprotein fractions included apo Bh (higher-molecular-weight apo B), apo B1 (lower-molecular-weight apo B), apo E, and apo C. Fasting decreased total hepatic apoprotein secretion from 75 to 53 μg·g−1·hr−1 and label incorporation into the apoproteins of very low density lipoproteins was reduced by 48% with the greatest reduction in both apo B fractions. There was a redistribution of apo Bh label into the low-density lipoproteins with fasting resulting in secretion of lipoproteins enriched in apo Bh. The high-density lipoprotein fraction contained 27 and 41% of the apo B1 label isolated from liver perfusates of fasted and fed animals, respectively. The nascent high-density lipoprotein was enriched in labeled apo B1 compared to low-density lipoproteins indicating that apo B in this density range represents a distinct metabolic fraction. With fasting the apo Bh and apo B1 apoproteins behave independently in terms of the distribution of label incorporation into nascent hepatic lipoproteins.
{"title":"The effect of fasting on the secretion of lipoproteins and two forms of apo B by perfused rat liver.","authors":"J B Marsh, C E Sparks","doi":"10.3181/00379727-170-41415","DOIUrl":"https://doi.org/10.3181/00379727-170-41415","url":null,"abstract":"Abstract Rat livers from animals fed ad lib or fasted 24 hr were perfused by the single pass technique with 3H-amino acids and label incorporation into lipoprotein apoproteins was measured. The lipoproteins were separated into very low density lipoproteins (d < 1.006), low-density lipoproteins (1.006 < d < 1.06), and high-density lipoproteins (1.06 < d < 1.21) by ultracentrifugation and the apoproteins of each density fraction were analyzed by gel filtration column chromatography in the presence of sodium dodecyl sulfate. The apoprotein fractions included apo Bh (higher-molecular-weight apo B), apo B1 (lower-molecular-weight apo B), apo E, and apo C. Fasting decreased total hepatic apoprotein secretion from 75 to 53 μg·g−1·hr−1 and label incorporation into the apoproteins of very low density lipoproteins was reduced by 48% with the greatest reduction in both apo B fractions. There was a redistribution of apo Bh label into the low-density lipoproteins with fasting resulting in secretion of lipoproteins enriched in apo Bh. The high-density lipoprotein fraction contained 27 and 41% of the apo B1 label isolated from liver perfusates of fasted and fed animals, respectively. The nascent high-density lipoprotein was enriched in labeled apo B1 compared to low-density lipoproteins indicating that apo B in this density range represents a distinct metabolic fraction. With fasting the apo Bh and apo B1 apoproteins behave independently in terms of the distribution of label incorporation into nascent hepatic lipoproteins.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"178-81"},"PeriodicalIF":0.0,"publicationDate":"1982-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-170-41415","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35262358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1982-06-01DOI: 10.3181/00379727-170-41420
M F Reilly, M K Bradley, S G Bradley
Abstract The capability of 154 serum samples from pediatric outpatients and 101 samples from adults to agglutinate amoebae of Naegleria fowleri nN68 was assessed. Sera from all 19 infants tested had an agglutination titer of 1:4 or less; sera of toddlers had a median agglutination titer of 1:8 and those of adults, 1:16. Only 13 of 154 serum samples from children had titers of 1:32 or 1:64; 7 of these high titered sera were from the 38 asthmatic children. Selected sera were found to give comparable titers when assayed against eight other strains of N. fowleri. Agglutination activity was removed by absorption with N. fowleri but not by absorption with N. gruberi. The agglutinating activity was specific for the human pathogenic N. fowleri. The ubiquitous free-living N. gruberi was not the immunogen responsible for the agglutinating activity in human serum.
{"title":"Agglutination of Naegleria fowleri by human serum.","authors":"M F Reilly, M K Bradley, S G Bradley","doi":"10.3181/00379727-170-41420","DOIUrl":"https://doi.org/10.3181/00379727-170-41420","url":null,"abstract":"Abstract The capability of 154 serum samples from pediatric outpatients and 101 samples from adults to agglutinate amoebae of Naegleria fowleri nN68 was assessed. Sera from all 19 infants tested had an agglutination titer of 1:4 or less; sera of toddlers had a median agglutination titer of 1:8 and those of adults, 1:16. Only 13 of 154 serum samples from children had titers of 1:32 or 1:64; 7 of these high titered sera were from the 38 asthmatic children. Selected sera were found to give comparable titers when assayed against eight other strains of N. fowleri. Agglutination activity was removed by absorption with N. fowleri but not by absorption with N. gruberi. The agglutinating activity was specific for the human pathogenic N. fowleri. The ubiquitous free-living N. gruberi was not the immunogen responsible for the agglutinating activity in human serum.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"209-12"},"PeriodicalIF":0.0,"publicationDate":"1982-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-170-41420","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35262360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1982-06-01DOI: 10.3181/00379727-170-41419
D R Garris
Abstract The ability of estradiol and prolactin to substitute for LH as luteotrophins was investigated in Day 5 (Day 1 = ovulation)-hysterectomized pseudopregnant (PSP) rats. All animals received either a sc injection of oil, 1 μg estradiol (E-1), or 150 μg prolactin (PRL) on Day 9 in combination with either a normal horse serum (NHS) or LH antiserum (LHAS) injection. The ability of oil, E-1, or PRL to maintain luteal function was assessed by monitoring serum progesterone levels through Day 12 and their direct effects on the ovary monitored by measuring luteal and extra-luteal ovarian tissue concentrations of estradiol and progesterone on Day 12. NHS treatment in combination with either oil, E-1, or PRL maintained elevated serum progesterone levels through Day 12 of PSP. LHAS/oil-treated rats underwent luteolysis while E-1 and PRL effectively maintained luteal function in LHAS-treated animals. PRL raised luteal and extraluteal ovarian estradiol concentrations compared to oil/NHS-treated controls and LHAS/oil-treated rats. E-1 induced an intraluteal rise in estradiol levels in LHAS-treated rats. While luteal progesterone concentrations fell following oil/LHAS treatment, E-1 and PRL effectively counteracted this LHAS-induced fall. The results of these studies indicate that (i) E-1 and PRL can effectively replace LH as luteotrophins during PSP in the rat and (ii) that PRL and E-1 effectively maintain luteal levels of estradiol and progesterone following LH deprivation. It is suggested that E-1 and PRL may exert their luteotrophic actions by either an E-1-induced increase in PRL which, in turn, acts on the luteal cells to increase intraluteal estradiol concentrations, or by a direct effect of E on the corpus luteum to bypass the requirement for LH.
{"title":"The luteotrophic effects of estradiol and prolactin in the absence of LH in the hysterectomized, pseudopregnant rat.","authors":"D R Garris","doi":"10.3181/00379727-170-41419","DOIUrl":"https://doi.org/10.3181/00379727-170-41419","url":null,"abstract":"Abstract The ability of estradiol and prolactin to substitute for LH as luteotrophins was investigated in Day 5 (Day 1 = ovulation)-hysterectomized pseudopregnant (PSP) rats. All animals received either a sc injection of oil, 1 μg estradiol (E-1), or 150 μg prolactin (PRL) on Day 9 in combination with either a normal horse serum (NHS) or LH antiserum (LHAS) injection. The ability of oil, E-1, or PRL to maintain luteal function was assessed by monitoring serum progesterone levels through Day 12 and their direct effects on the ovary monitored by measuring luteal and extra-luteal ovarian tissue concentrations of estradiol and progesterone on Day 12. NHS treatment in combination with either oil, E-1, or PRL maintained elevated serum progesterone levels through Day 12 of PSP. LHAS/oil-treated rats underwent luteolysis while E-1 and PRL effectively maintained luteal function in LHAS-treated animals. PRL raised luteal and extraluteal ovarian estradiol concentrations compared to oil/NHS-treated controls and LHAS/oil-treated rats. E-1 induced an intraluteal rise in estradiol levels in LHAS-treated rats. While luteal progesterone concentrations fell following oil/LHAS treatment, E-1 and PRL effectively counteracted this LHAS-induced fall. The results of these studies indicate that (i) E-1 and PRL can effectively replace LH as luteotrophins during PSP in the rat and (ii) that PRL and E-1 effectively maintain luteal levels of estradiol and progesterone following LH deprivation. It is suggested that E-1 and PRL may exert their luteotrophic actions by either an E-1-induced increase in PRL which, in turn, acts on the luteal cells to increase intraluteal estradiol concentrations, or by a direct effect of E on the corpus luteum to bypass the requirement for LH.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"203-8"},"PeriodicalIF":0.0,"publicationDate":"1982-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-170-41419","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35262359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1982-06-01DOI: 10.3181/00379727-170-41421
G D Kalmaz, T P McDonald
Abstract A sensitive assay for a thrombocytopoiesis-stimulating factor (TSF or thrombopoietin) that utilizes measurement of the small acetylcholinesterase-positive (SAChE+) cell in the marrow of mice is described. The results of this assay were compared with a previously published procedure that measures 35S incorporation into platelets of immuno-thrombocythemic mice. In the rebound-thrombocytotic mouse assay, TSF from kidney cell culture medium caused a dose-related increase in the amount of 35S incorporation into platelets; the minimum detectable dose of TSF was 15 mg of protein/mouse. For the SAChE+ cell assay, mice received single intraperitoneal injections of TSF or platelet-specific antiserum. Other mice were injected with plasma from thrombocytopenic donor mice. For controls, groups of mice were given saline or normal mouse plasma. Bone marrow from the mice (killed at 10 hr after injection) was taken for smears and stained for acetylcholinesterase. The results show that both TSF and rabbit anti-mouse platelet serum (RAMPS) caused a highly significant dose-dependent increase in the percentage of SAChE+ cells and that plasma from thrombocytopenic mice stimulated an increase (P < 0.0005) in the percentage of SAChE+ cells. The minimum detectable dose of TSF in the SAChE+ cell assay was 1.875 mg protein/mouse. Therefore, SAChE+ cells in the marrow of mice can detect smaller doses of TSF than the rebound-thrombocytotic mouse assay.
{"title":"Assay for thrombopoietin: a new, more sensitive method based on measurement of the small acetylcholinesterase-positive cell.","authors":"G D Kalmaz, T P McDonald","doi":"10.3181/00379727-170-41421","DOIUrl":"https://doi.org/10.3181/00379727-170-41421","url":null,"abstract":"Abstract A sensitive assay for a thrombocytopoiesis-stimulating factor (TSF or thrombopoietin) that utilizes measurement of the small acetylcholinesterase-positive (SAChE+) cell in the marrow of mice is described. The results of this assay were compared with a previously published procedure that measures 35S incorporation into platelets of immuno-thrombocythemic mice. In the rebound-thrombocytotic mouse assay, TSF from kidney cell culture medium caused a dose-related increase in the amount of 35S incorporation into platelets; the minimum detectable dose of TSF was 15 mg of protein/mouse. For the SAChE+ cell assay, mice received single intraperitoneal injections of TSF or platelet-specific antiserum. Other mice were injected with plasma from thrombocytopenic donor mice. For controls, groups of mice were given saline or normal mouse plasma. Bone marrow from the mice (killed at 10 hr after injection) was taken for smears and stained for acetylcholinesterase. The results show that both TSF and rabbit anti-mouse platelet serum (RAMPS) caused a highly significant dose-dependent increase in the percentage of SAChE+ cells and that plasma from thrombocytopenic mice stimulated an increase (P < 0.0005) in the percentage of SAChE+ cells. The minimum detectable dose of TSF in the SAChE+ cell assay was 1.875 mg protein/mouse. Therefore, SAChE+ cells in the marrow of mice can detect smaller doses of TSF than the rebound-thrombocytotic mouse assay.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"213-9"},"PeriodicalIF":0.0,"publicationDate":"1982-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-170-41421","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35262361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1965-05-01DOI: 10.3181/00379727-119-30099
E R FISHER, B FISHER
Summary Tensile strength and rate of contraction of standard wounds were comparable in neonatally thymectomized and intact control Sprague-Dawley rats. Quantitative and qualitative histochemical and histological estimation of fibrillar, substrate and exudative stages of wounding were also similar. It is concluded that lymphocytes exert no thymus-dependent immunologic influence on this type of inflammatory response.
{"title":"LACK OF THYMIC EFFECT ON WOUND HEALING.","authors":"E R FISHER, B FISHER","doi":"10.3181/00379727-119-30099","DOIUrl":"https://doi.org/10.3181/00379727-119-30099","url":null,"abstract":"Summary Tensile strength and rate of contraction of standard wounds were comparable in neonatally thymectomized and intact control Sprague-Dawley rats. Quantitative and qualitative histochemical and histological estimation of fibrillar, substrate and exudative stages of wounding were also similar. It is concluded that lymphocytes exert no thymus-dependent immunologic influence on this type of inflammatory response.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"61-3"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40796577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1965-05-01DOI: 10.3181/00379727-119-30087
J J SULLIVAN, A C MENGE
Summary The uptake of triiodothyronine-I-131 by washed bull spermatozoa was found to have a positive association with sperm cell concentration and period of incubation with the labeled T3. Spermatozoa suspended in a simple salt solution (2.9% sodium citrate) had a higher uptake of T3 131 than spermatozoa diluted in a more complex ionic medium (N-J solution). The T3-I131 uptake by the sperm cells suspended in the 2 diluents increased at different rates with an increase in cell concentration. Within the range studied, addition of glucose to the 2 diluents had no effect on uptake of T3-I131 by spermatozoa. Sperm cell viability greatly influenced their uptake of T3-I131. Cells killed by continuous incubation at 38 °C or by treatment with acetic acid or sodium hydroxide had similar isotope uptake values which were significantly less than that of live cells. Spermatozoa killed by cold shock treatment, however, had the lowest uptake of T3-I131. Whereas, formaldehyde treatment of spermatozoa resulted in uptake values significantly greater than that of live cells. A positive association between uptake of T3-I131 and motility occurred with spermatozoa stored up to 18 days in different diluents and temperatures.
{"title":"FACTORS INFLUENCING UPTAKE OF TRIIODOTHYRONINE-I-131 BY BULL SPERMATOZOA.","authors":"J J SULLIVAN, A C MENGE","doi":"10.3181/00379727-119-30087","DOIUrl":"https://doi.org/10.3181/00379727-119-30087","url":null,"abstract":"Summary The uptake of triiodothyronine-I-131 by washed bull spermatozoa was found to have a positive association with sperm cell concentration and period of incubation with the labeled T3. Spermatozoa suspended in a simple salt solution (2.9% sodium citrate) had a higher uptake of T3 131 than spermatozoa diluted in a more complex ionic medium (N-J solution). The T3-I131 uptake by the sperm cells suspended in the 2 diluents increased at different rates with an increase in cell concentration. Within the range studied, addition of glucose to the 2 diluents had no effect on uptake of T3-I131 by spermatozoa. Sperm cell viability greatly influenced their uptake of T3-I131. Cells killed by continuous incubation at 38 °C or by treatment with acetic acid or sodium hydroxide had similar isotope uptake values which were significantly less than that of live cells. Spermatozoa killed by cold shock treatment, however, had the lowest uptake of T3-I131. Whereas, formaldehyde treatment of spermatozoa resulted in uptake values significantly greater than that of live cells. A positive association between uptake of T3-I131 and motility occurred with spermatozoa stored up to 18 days in different diluents and temperatures.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"20-4"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40796822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1965-05-01DOI: 10.3181/00379727-119-30142
C V ROBINSON, S L COMMERFORD, J L BATEMAN
Summary In experiments in which the tails of mice were shielded during exposure to X-rays, colonization of the spleen occurred in excess of that found in whole-body irradiated control animals. This was the case whether the measure of effect was post-irradiative gain in spleen weight, uptake of IDU (5-iodo-2′-deoxyuridine), or nodule count. The degree of colonization was found to be a function of the length of time which elapsed between the original irradiation (with tail shielded) and a subsequent, suppressive irradiation of the tail. Histological examination showed that the spleen nodules were hemopoietic, and that normal tail vertebrae contained hemopoietic tissue.
{"title":"EVIDENCE FOR THE PRESENCE OF STEM CELLS IN THE TAIL OF THE MOUSE.","authors":"C V ROBINSON, S L COMMERFORD, J L BATEMAN","doi":"10.3181/00379727-119-30142","DOIUrl":"https://doi.org/10.3181/00379727-119-30142","url":null,"abstract":"Summary In experiments in which the tails of mice were shielded during exposure to X-rays, colonization of the spleen occurred in excess of that found in whole-body irradiated control animals. This was the case whether the measure of effect was post-irradiative gain in spleen weight, uptake of IDU (5-iodo-2′-deoxyuridine), or nodule count. The degree of colonization was found to be a function of the length of time which elapsed between the original irradiation (with tail shielded) and a subsequent, suppressive irradiation of the tail. Histological examination showed that the spleen nodules were hemopoietic, and that normal tail vertebrae contained hemopoietic tissue.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"222-6"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40796826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1965-05-01DOI: 10.3181/00379727-119-30143
F I LOTTSFELDT, R F LABBE
Summary The treatment of rats with allylisopropylacetamide resulted in a doubling of liver size within 3 days. Microscopic examination showed an increase in cell size and less increase in cell number per liver. By chemical analyses, there were found net gains in RNA and DNA per liver which paralleled the size and number of cells respectively. In per cent composition, lipids doubled while other components examined remained unchanged. It was concluded that the cytologic effect of the drug may be 2-fold, resulting primarily in cellular hypertrophy, but also in cellular hyperplasia.
{"title":"SOME CYTOLOGIC CHANGES OF RAT LIVER IN EXPERIMENTAL PORPHYRIA.","authors":"F I LOTTSFELDT, R F LABBE","doi":"10.3181/00379727-119-30143","DOIUrl":"https://doi.org/10.3181/00379727-119-30143","url":null,"abstract":"Summary The treatment of rats with allylisopropylacetamide resulted in a doubling of liver size within 3 days. Microscopic examination showed an increase in cell size and less increase in cell number per liver. By chemical analyses, there were found net gains in RNA and DNA per liver which paralleled the size and number of cells respectively. In per cent composition, lipids doubled while other components examined remained unchanged. It was concluded that the cytologic effect of the drug may be 2-fold, resulting primarily in cellular hypertrophy, but also in cellular hyperplasia.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"226-9"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40796827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1965-05-01DOI: 10.3181/00379727-119-30144
R R FOX, E J BABINO
Summary Pathological and preclinical stages in the buphthalmic rabbit's eye can be distinguished by the degree of corneal cornification. This is determined by enumeration of cells removed from the intact anesthetized eye. Five normal strains and 2 buphthalmic strains were investigated. The buphthalmics had cell counts significantly higher than those of genetically normal animals. No significant differences were observed either between any of the control strains or between buphthalmic groups.
{"title":"BUPHTHALMIA IN THE RABBIT: A TEST FOR EARLY DIAGNOSIS.","authors":"R R FOX, E J BABINO","doi":"10.3181/00379727-119-30144","DOIUrl":"https://doi.org/10.3181/00379727-119-30144","url":null,"abstract":"Summary Pathological and preclinical stages in the buphthalmic rabbit's eye can be distinguished by the degree of corneal cornification. This is determined by enumeration of cells removed from the intact anesthetized eye. Five normal strains and 2 buphthalmic strains were investigated. The buphthalmics had cell counts significantly higher than those of genetically normal animals. No significant differences were observed either between any of the control strains or between buphthalmic groups.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"229-33"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30144","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40796828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1965-05-01DOI: 10.3181/00379727-119-30147
I C WELLS, J M BUCKLEY
Summary The results obtained by the analyses of bile, collected from normal and choline deficient male rats during the first 24 hours after cannulation of the bile duct, indicate that the volume of bile produced is not changed but the concentrations of lipid components are generally decreased. Esterified cholesterol was absent and free cholesterol was in the same concentration as normal in the deficient bile. These findings may explain the previously observed accumulation of cholesterol esters in the livers of choline deficient rats.
{"title":"LIPID COMPONENTS OF BILE FROM CHOLINE DEFICIENT RATS.","authors":"I C WELLS, J M BUCKLEY","doi":"10.3181/00379727-119-30147","DOIUrl":"https://doi.org/10.3181/00379727-119-30147","url":null,"abstract":"Summary The results obtained by the analyses of bile, collected from normal and choline deficient male rats during the first 24 hours after cannulation of the bile duct, indicate that the volume of bile produced is not changed but the concentrations of lipid components are generally decreased. Esterified cholesterol was absent and free cholesterol was in the same concentration as normal in the deficient bile. These findings may explain the previously observed accumulation of cholesterol esters in the livers of choline deficient rats.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"242-3"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40796831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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