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Synthesis, Characterization and Study Biological Activity of Substituted 4-Amino -3,5-Bis (2,4-dichloro phenoxy)-1,2,4-Triazole 取代型 4-氨基-3,5-双(2,4-二氯苯氧基)-1,2,4-三唑的合成、表征和生物活性研究
Pub Date : 2024-03-31 DOI: 10.33899/rjs.2024.182834
Omar Yahya
In this paper, the ester (2) was prepared by reacting 2,4-dichlorophenoxy acetic acid with methyl alcohol and using concentrated sulfuric acid as a catalyst in this reaction, which, when reacted with hydrazine hydrate, obtained acid hydrazide. (3), under reflux conditions with dimethyl sulfoxide and at solvent temperature, the compound 4-amino-3,5-bis-(2,4-dichlorophenoxy)-1,2,4-triazole was obtained (4). It was obtained and used to prepare a series of new Schiff bases (5a-e) through their reaction with different substitutes of benzaldehyde. The completeness and purity of all the prepared compounds were confirmed by thin-layer chromatography (TLC) and showed some physical and spectroscopic properties, namely (FT -IR 1H-NMR and 13C-NMR). In addition, the biological activity of the prepared compounds was studied, and their effectiveness against different types of bacteria such as Bacillus, Escherichia coli , and Staphylococcus aureus was discussed. Studies of mainly fungi and bacteria have shown good efficacy against the mentioned species using disc diffusion technology.
在本文中,酯(2)是通过 2,4-二氯苯氧基乙酸与甲醇反应制备的,在该反应中使用浓硫酸作为催化剂,当与水合肼反应时,得到酸肼。(3) 在溶剂温度下,用二甲亚砜进行回流,得到 4-氨基-3,5-双-(2,4-二氯苯氧基)-1,2,4-三唑化合物 (4)。通过与不同的苯甲醛替代物反应,得到了 4-氨基-3,5-双-(2,4-二氯苯氧基)-1,2,4-三唑(4)。通过薄层色谱法(TLC)确认了所有制备化合物的完整性和纯度,并显示了一些物理和光谱特性,即(傅立叶变换红外光谱 1H-NMR 和 13C-NMR)。此外,还研究了所制备化合物的生物活性,并讨论了它们对不同类型细菌(如芽孢杆菌、大肠杆菌和金黄色葡萄球菌)的有效性。利用圆盘扩散技术对真菌和细菌进行的研究表明,这些化合物对上述菌种具有良好的疗效。
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引用次数: 0
Theoretical Investigation on Transportance for Single-Walled Nanotubes, and Bi-Layer Graphene 单壁纳米管和双层石墨烯迁移率的理论研究
Pub Date : 2024-03-31 DOI: 10.33899/rjs.2024.182836
Maher Abdullah
The transport properties of several carbon nanoparticle derivatives, including single-layer nanotubes and bi-layer graphene, under an external constant electric field, are investigated. Results were analyzed and identified as a function of the quasi-classical approximation of relaxation time by using the Boltzmann kinetic equations. These approaches have been used to obtain the relationships between the electric conductivity σ( E ) and electrons diffusion coefficient D ( E ) in carbon nanostructures. This study proves that when the temperature rises the electrical conductivity σ( E ) decreases for both single-layer and bi-layer carbon nanostructures. For a wide range of temperatures, the electrical conductivity σ (E) behavior of the studied nanoparticles is nonlinearly dependent on the magnitude of the external electric field ( E ). In contrast, the electron diffusion coefficient D ( E ) is independent of temperature for both single-and bi-layers. It also shows that the intensity of an external electric field possesses a nonlinear influence on the electron diffusion coefficient.
研究了单层纳米管和双层石墨烯等几种碳纳米粒子衍生物在外部恒定电场下的传输特性。利用玻尔兹曼动力学方程分析并确定了结果与弛豫时间准经典近似值的函数关系。这些方法被用来获得碳纳米结构中电导率 σ( E ) 和电子扩散系数 D ( E ) 之间的关系。这项研究证明,当温度升高时,单层和双层碳纳米结构的导电率 σ( E ) 都会降低。在很宽的温度范围内,所研究纳米粒子的电导率σ(E)行为与外部电场(E)的大小呈非线性关系。相反,单层和双层的电子扩散系数 D ( E ) 与温度无关。这也表明外电场强度对电子扩散系数具有非线性影响。
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引用次数: 0
Studies on Kinetic Properties of Aspergillus Producing Peroxidase from Petroleum Hydrocarbon Spilled Soil 石油烃泄漏土壤中产生过氧化物酶的曲霉的动力学特性研究
Pub Date : 2024-03-31 DOI: 10.33899/rjs.2024.182828
Chijioke Ezenwelu, Kingsley Agu, Rita Ngwu, Joy Ogana, Emeka Oparaji
Peroxidases production was carried out from Aspergillus tamari isolated from petroleum hydrocarbon spilled soil. Physicochemical properties of the respective soil showed pH of 4.45 and 6.5 for soils from point 1 and II respectively and higher conductivity of 613 and 1013 (Ω -1 Cm -1) , respectively when compared with the control sample. Dissolved mineral of Cl - , SO 4 , K, Ca, Mg in the respective soil samples from the petroleum spilled sites were significantly high when compared with the control experiment except for soil sample I which showed a relative low phosphate concentration of 1.23 mg/g in the presence of the control experiment, respectively. TOC and TOM contents were 87.91, 119.04; 108.13 and 146.42 mg/g for soil sample I, and III, respectively. In all the tested parameters, the experimented soils were significantly high than the control soil sample. Molecular tests (18s rDNA.) was used to identify the pure isolate of fungus as Aspergillus tamarii. Studies on effect of incubation period on the production of peroxidase from strains of Aspergillus tamarrii sp. showed that the highest peroxidase activity was obtained on the day 6 th of the fermentation time; Peroxidase activity peaked at pH 5. Protein with highest peroxidase activity was peak precipitated at 60% saturation of the salt. The gel chroـmatogram showed single almost superimposed peaks of enzyme activity. Purified peroxidase activity peaked at pH 4.5. Optimum temperature for the enzyme activity was at 50 °C. Km and V max of 3.45mM and 280 μmole/min was extrapolated from the reciprocal curve of Lineweaver-burke at various concentrations of 2,6 DMP. Fe, Ca, Co and Mn selected as their notable impact in the active site of peroxidase guided the selected were assayed in the presence of the enzymes, respectively. The stability curve obtained for the peroxidases was single biphasic which represent the first order; the enzymes maintained greater than 50% of their activity after 30 min of incubation as activity progressively decreased upto to 40% after 60 min of incubation. Thermal stability of peroxidase at its optimum temperature (50) and at 70°C showed a biphasic stability curve. The enzymes maintained greater than 50% of their activity after 60 min of incubation.
从石油烃泄漏的土壤中分离出的 Tamari 曲霉产生了过氧化物酶。与对照样本相比,1 号点和 II 号点土壤的 pH 值分别为 4.45 和 6.5,电导率分别为 613 和 1013 (Ω -1 Cm -1) 。与对照实验相比,石油泄漏点各土壤样本中的 Cl -、SO 4、K、Ca、Mg 等溶解矿物质含量明显偏高,只有土壤样本 I 的磷酸盐含量相对较低,仅为 1.23 mg/g。土壤样品 I 和 III 的 TOC 和 TOM 含量分别为 87.91、119.04、108.13 和 146.42 mg/g。在所有测试参数中,实验土壤都明显高于对照土壤样本。通过分子检测(18s rDNA),确定纯分离的真菌为 Tamarii 曲霉。培养期对塔马瑞曲霉菌株产生过氧化物酶的影响研究表明,发酵第 6 天时过氧化物酶活性最高;过氧化物酶活性在 pH 值为 5 时达到峰值。凝胶络˙图显示酶活性的单峰几乎重叠。纯化的过氧化物酶活性在 pH 值为 4.5 时达到峰值。酶活性的最佳温度为 50 °C。在不同浓度的 2,6 DMP 下,根据 Lineweaver-burke 的倒数曲线推断,Km 和 V max 分别为 3.45mM 和 280 μmole/min。由于铁、钙、钴和锰对过氧化物酶活性位点有显著影响,因此分别在酶的存在下对所选的过氧化物酶进行了测定。过氧化物酶的稳定性曲线是单双相的,代表一阶;孵育 30 分钟后,酶的活性保持在 50%以上,而孵育 60 分钟后,活性逐渐下降到 40%。过氧化物酶在最适温度(50)和 70°C 下的热稳定性呈现双相稳定曲线。孵育 60 分钟后,酶的活性保持在 50%以上。
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引用次数: 0
Estimation of some Inflammatory Markers in Covid-19 Patients in Erbil / City 埃尔比勒市 Covid-19 患者某些炎症指标的估算
Pub Date : 2024-03-31 DOI: 10.33899/rjs.2024.182821
shayma Muhammed, S. Darogha
The 2019 pandemic coronavirus disease has affected millions of individuals globally. The objective of the present study is to assess the possible predictors of CoV-2 severity and to identify the possible correlation between patients' parameters and disease severity, Consequently, we aimed to measure the serum levels of some inflammatory markers, including CRP, D-dimer, Ferritin, and Procalcitonin, as a biomarker for disease severity in CoV-2 patients. A total of 280 nasopharyngeal swabs and whole blood specimens were collected from healthy individuals and individuals suspected with CoV-2 between June 2021 and December 2021 of both sexes, categorized into four main groups: 70 healthy individuals with an age range (23-70), 210 CoV-2 patients in which their ages were between (21-75), (70 patients per mild, moderate and severe patients). According to our findings CoV-2 patients’ groups had leukocytosis, with a significant increase in WBC and Granulocytes count, and a significant decrease in Lymphocyte and platelet. In regard to inflammatory parameters, CRP, D-dimer, ferritin and PCT showed significant differences between the CoV-2 patients groups compare to the control group, these inflammatory biomarkers were significantly elevated in CoV-2 patients group compared to healthy control group (P<0.005). The optimal cut-off values for CRP, D-dimer, Ferritin, and PCT were determined by Receiver Operating Characteristic (ROC) Curve Analysis in CoV-2 patients. In conclusion, Inflammation biomarkers are the best predictors of severe CoV-2, and the combination of clinical signs can further predict severe CoV-2.
2019年大流行的冠状病毒疾病已影响到全球数百万人。本研究旨在评估CoV-2严重程度的可能预测因素,并确定患者参数与疾病严重程度之间可能存在的相关性。因此,我们旨在测量一些炎症标志物的血清水平,包括CRP、D-二聚体、铁蛋白和降钙素原,作为CoV-2患者疾病严重程度的生物标志物。我们在 2021 年 6 月至 2021 年 12 月期间从健康人和 CoV-2 疑似患者中收集了 280 份鼻咽拭子和全血标本,将其分为四大类:70 名健康人,年龄在 23-70 岁之间;210 名 CoV-2 患者,年龄在 21-75 岁之间(轻度、中度和重度患者各 70 人)。根据我们的研究结果,CoV-2 患者组白细胞增多,白细胞和粒细胞计数显著增加,淋巴细胞和血小板显著减少。在炎症指标方面,CRP、D-二聚体、铁蛋白和 PCT 在 CoV-2 患者组与对照组之间存在显著差异,与健康对照组相比,CoV-2 患者组的这些炎症生物标志物明显升高(P<0.005)。CoV-2患者的CRP、D-二聚体、铁蛋白和PCT的最佳临界值是通过接收者操作特征曲线(ROC)分析确定的。总之,炎症生物标志物是预测重症CoV-2的最佳指标,结合临床症状可进一步预测重症CoV-2。
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引用次数: 0
Alizarin Red S as a Chromogenic Agent for the Determination of Meropenem in Pharmaceutical- Formulations 茜素红 S 作为致色剂测定药物制剂中的美罗培南
Pub Date : 2024-03-31 DOI: 10.33899/rjs.2024.182833
Wallada H. Ibrahim, Hana Mahmood
Alizarin red S (ALRS)reagent has been used for to determination of meropenem (MEP)by an easy, sensitive, and selective procedure based on an ion-pair formation reaction between ALRS and MEP in an aqueous: alcoholic medium in the ratio of 50:50 without adjustment of the acidity of the reaction medium. The linearity ranged from 1 to 50 µg /mL, molar absorptivity was 6.557 × 10 3 L.mol -1 .cm -1 ., index of Sandell’s, was 585584 µg.cm -2 , and the calculated LOD and LOQ were 0.02789 µg/mL, 0.0929 µg/mL respectively. The calculated range of relative error was from -1.022 to 0.34 % which indicates high accuracy, and the calculated range of the relative standard deviation was from 0.347 to 0.574% which indicates high precision. The method has been applied for the determination of MEP in dosage forms successfully in which the recovery ranged from 99.8 to 103.2%. The standard addition method proves no interfering effect caused by inactive ingredients involved in dosage forms of meropenem
茜素红 S(ALRS)试剂用于美罗培南(MEP)的测定,该方法简便、灵敏、选择性好,ALRS与MEP在水:醇介质中以50:50的比例发生离子对反应,无需调节反应介质的酸度。线性范围为 1 至 50 µg /mL,摩尔吸收率为 6.557 × 10 3 L.mol -1 .cm -1 .,桑德尔指数为 585584 µg.cm -2 ,计算的最低检测限和最低定量限分别为 0.02789 µg/mL 和 0.0929 µg/mL。相对误差的计算范围为-1.022%~0.34%,准确度较高;相对标准偏差的计算范围为 0.347%~0.574%,精密度较高。该方法被成功地用于测定剂型中的 MEP,回收率在 99.8%至 103.2%之间。标准添加法证明,美罗培南剂型中的非活性成分不会产生干扰效应。
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引用次数: 0
Trends Analysis of Mean Temperature in Iraq 伊拉克平均气温趋势分析
Pub Date : 2024-03-31 DOI: 10.33899/rjs.2024.182840
Mustafa Aljaff, Hani Mohammed Salih AL-JIRJEES
In this study, the trend of air temperatures is investigated by employing measured and predictive data of maximum and minimum temperatures for daily and annual means in three synoptic stations, geographically and climatically distributed in different regions, as follows: (Mosul, Baghdad and Basra), for time series (1982-2020) of measured means up to (2021-2050) for predictive data from Coupled Model Intercomparison Project Phase 6 (CMIP6). Mann-Kendall analysis, which is a statistical test widely used in climate series to determine the statistical significance of the monotonic trend of temperature means, showed that maximum, daily, and annual temperature means had a positive trend and were statistically significant at the significance level (  = 0.05) and a non-significant fluctuating trend in minimum daily and annual. A high increase in the annual differences with a range of (-1.47-7.25 ℃ ) between the beginning and end of the measured daily time series (1982–2020) that showed a high increase in Basra, followed by the Mosul station, with an increase of the range (5.37-1.24 ℃ ), and finally for the Baghdad station, the increase was recorded within the range (1.24-5.37 ℃ ). Overall, under the potential of global warming, the results concluded that trends of mean temperatures appeared to be in the best agreement with the extreme scenario (SSP5-8.5) compared to the scenario (SSP2-4.5) till the middle of the present century. Globally, the region topped the regions of extreme temperature risks under the governmental authorities' entire deficit to set optimal solutions for climate change and extreme events in Iraq.
在本研究中,通过使用分布在不同地区的三个同步站的最高和最低气温的日平均值和年平均值的实测和预测数据,对气温趋势进行了研究:(摩苏尔、巴格达和巴士拉)的时间序列(1982-2020 年)的实测平均气温,以及(2021-2050 年)来自耦合模式相互比较项目第 6 阶段(CMIP6)的预测数据。曼-肯德尔(Mann-Kendall)分析是气候序列中广泛使用的一种统计检验方法,用于确定气温平均值单调趋势的统计意义。该分析表明,最高气温、日平均气温和年平均气温呈正趋势,在显著性水平( = 0.05)上具有统计意义,而日最低气温和年最低气温的波动趋势不显著。在测量的日时间序列(1982-2020 年)的起点和终点之间,年较差的增幅范围为(-1.47-7.25 ℃),其中巴士拉的增幅较大,其次是摩苏尔站,增幅范围为(5.37-1.24 ℃),最后是巴格达站,增幅范围为(1.24-5.37 ℃)。总之,在全球变暖的潜在影响下,研究结果表明,到本世纪中叶,平均气温的变化趋势与极端情景(SSP5-8.5)相比,似乎与情景(SSP2-4.5)最为一致。在全球范围内,该地区在极端气温风险地区中名列前茅,而政府当局在为伊拉克的气候变 化和极端事件制定最佳解决方案方面存在全面缺陷。
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引用次数: 0
A review:: Viruses and their Relation with Breast Cancer 综述::病毒及其与乳腺癌的关系
Pub Date : 2023-12-01 DOI: 10.33899/rjs.2023.181499
Zeyad T. Al-Rassam, Watban Hameed, Ahed Aliawi
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引用次数: 0
The Effect of some Parameters of the Solar Wind on the Amounts of Clouds-Over Iraq for the Period (1988-2019) 1988-2019年太阳风参数对伊拉克云量的影响
Pub Date : 2023-09-01 DOI: 10.33899/rjs.2023.180293
Anfal ALJeboury, Imad Al-Ibrahimi, Igor Kopytin
It is known that the sun and its phenomena have an effect on the Earth's atmosphere in general and the troposphere in particular Especially from this pulpit and because of the importance of this topic and the studies that included it on Iraq, there were few studies to find the relationship between some The parameters of the solar wind are represented by (the radio flux of the sun, F10.7, the magnetic field DST and the electric field Ey) and their effect on the amounts of clouds above Iraq for the period 10/1/1988-31/5/2019. Solar wind parameters data were adopted from the official website of NASA (NOAA), at When clouds quantities were adopted from the Iraqi general authority for Meteorology and Seismic Monitoring for four different stations (Mosul and Kirkuk represent the northern region - Baghdad represents the central region - Basra represents the southern region. The data were statistically analyzed by the statistical program (Minitab 19.0), where the results showed that the effect of F10.7 is generally opposite, except for the phase descending from solar cycle 24 does not show an effect. DST is fluctuating in effect as it is reversed in the total duration and descending phase of the solar cycle 24 and is directed to the solar cycle 23 and does not show the effect of the solar cycle 22 While the electric field Ey has a direct effect for the total duration and an inverse effect with respect to the descending phase of the solar cycle 23.
众所周知,太阳及其现象一般对地球大气层,特别是对流层有影响,特别是在这个讲坛上,由于这一主题的重要性以及包括它在内的关于伊拉克的研究,很少有研究发现一些太阳风参数之间的关系,太阳的无线电通量,F10.7,1988年10月1日至2019年5月31日期间伊拉克上空的磁场DST和电场Ey及其对云量的影响。太阳风参数数据来自美国国家航空航天局(NOAA)的官方网站,而云量则来自伊拉克气象和地震监测总局的四个不同站点(摩苏尔和基尔库克代表北部地区,巴格达代表中部地区,巴士拉代表南部地区)。通过统计程序(Minitab 19.0)对数据进行统计分析,结果表明,除第24太阳周期下降的相位没有影响外,F10.7的影响总体相反。夏时制实际上是波动的,因为它在太阳周期24的总持续时间和下降阶段是相反的,它指向太阳周期23,不显示太阳周期22的影响,而电场Ey对总持续时间有直接影响,对太阳周期23的下降阶段有相反的影响。
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引用次数: 0
Temperature Calculation for Plasma Spectrum Analysis using Maxwell-Boltzmann Distribution 用麦克斯韦-玻尔兹曼分布计算等离子体光谱分析的温度
Pub Date : 2023-09-01 DOI: 10.33899/rjs.2023.180292
Atheel Alaqa, Yaser Al-jwaady, Raied Al-Wazzan
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引用次数: 0
Effect the Static Magnetic Field on some Hematological Parameters of Human AML Leukemia: in vitro 静磁场对人AML白血病血液学指标的影响
Pub Date : 2023-09-01 DOI: 10.33899/rjs.2023.180274
Shaima Haitham Zaki Al-Qadi, Qusay Al-dulamey, Mazin Abed, Ahmed Mehuaiden
In contrast to its relative effects on PLT and white blood cells, the percentage of the magnetic field's influence on red blood cells in this study is lower. The behavior of PLT and RBC are identical (decrease relativity after exposure) at low dose rates of 0.1 Tesla/ hr. and high dose rates of 0.7 Tesla/ hr., and they exhibit opposite behaviors to those of WBC. The ratio of PLT and RBC reaches the high ratio for both males and females at the dose rate of 0.4 Tesla/hr. So, the best exposure dose rate for PLT, WBC, and RBC is 0.4 Tesla/hr. Because these components are radiosensitive, the reversal of the AML leukemia blood components of the incident magnetic field changes. Additionally, cancer cells experience higher levels of ionization than healthy cells. Therefore, the ratio of PLT and RBC after exposure rose at the high-dose rate (0.7 Tesla/hr.). This is so because healthy cells are also included in the damage rate. It is obvious that leukemia blood samples exhibit distinct PLT, WBC, and RBC behaviors than healthy blood samples. This is a result of the increased rate of ionizations during the radiation treatment of blood samples with leukemia. This indicates that the rates of ionization for blood samples containing leukemia and healthy blood are different. The findings are consistent with the fundamental ideas underlying the phenomenon of biological radiation interaction.
与磁场对PLT和白细胞的相对影响相比,本研究中磁场对红细胞的影响百分比较低。在0.1特斯拉/小时的低剂量率下,PLT和RBC的行为是相同的(暴露后相关性降低)。高剂量率为0.7特斯拉/小时。,表现出与白细胞相反的行为。在0.4特斯拉/小时的剂量率下,男性和女性的PLT和RBC比值均达到较高。因此,血小板、白细胞和红细胞的最佳暴露剂量率为0.4特斯拉/小时。由于这些成分具有放射敏感性,逆转了AML白血病血液成分的入射磁场变化。此外,癌细胞比健康细胞经历更高水平的电离。因此,在高剂量率(0.7特斯拉/小时)下,暴露后PLT和RBC的比值升高。这是因为健康细胞也包括在损伤率中。很明显,白血病血液样本比健康血液样本表现出不同的PLT、WBC和RBC行为。这是白血病血液样本放射治疗期间电离率增加的结果。这表明含有白血病和健康血液样本的电离率是不同的。这些发现与生物辐射相互作用现象的基本观点是一致的。
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引用次数: 1
期刊
Rafidain journal of science
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