Pub Date : 2023-03-01DOI: 10.33899/rjs.2023.177285
M. Al-Allaf, A. AL-Rawi, Kossay AL-Ahmadi
Nanoparticles biosynthesis has gained great importance as an active eco-friendly method with economic benefit which overcame on other chemical and physical methods. This research involved green biosynthesis of iron oxide nanoparticles (IONPs) using Escherichia coli ( E.coli ) isolated from wastewater in Mosul city. Characterization of nanoparticles was performed by using many techniques which included UV-Vis Spectroscopy, Scanning Electron Microscope (SEM), Atomic Force Microscope (AFM), X-Ray Diffraction (XRD) and Fourier Transforms Spectroscopy (FTIR). Designing of a locally lab scale wastewater treatment plant was done by using IONPs adding to a dual water purification system, after tightly wrapping the filter with (1%) of IONPs solution up to saturation. Moreover, control filter was used. Sample of wastewater was passed through these filters to detect its effect on wastewater quality, the results showed that NPs filters improved physical, chemical and biological properties of wastewater including total plate count, coliform, fecal coliform and total fungi.
{"title":"Biosynthesis of Iron Oxide Nanoparticles and Using it to Support the Efficiency of Wastewater Filtration System","authors":"M. Al-Allaf, A. AL-Rawi, Kossay AL-Ahmadi","doi":"10.33899/rjs.2023.177285","DOIUrl":"https://doi.org/10.33899/rjs.2023.177285","url":null,"abstract":"Nanoparticles biosynthesis has gained great importance as an active eco-friendly method with economic benefit which overcame on other chemical and physical methods. This research involved green biosynthesis of iron oxide nanoparticles (IONPs) using Escherichia coli ( E.coli ) isolated from wastewater in Mosul city. Characterization of nanoparticles was performed by using many techniques which included UV-Vis Spectroscopy, Scanning Electron Microscope (SEM), Atomic Force Microscope (AFM), X-Ray Diffraction (XRD) and Fourier Transforms Spectroscopy (FTIR). Designing of a locally lab scale wastewater treatment plant was done by using IONPs adding to a dual water purification system, after tightly wrapping the filter with (1%) of IONPs solution up to saturation. Moreover, control filter was used. Sample of wastewater was passed through these filters to detect its effect on wastewater quality, the results showed that NPs filters improved physical, chemical and biological properties of wastewater including total plate count, coliform, fecal coliform and total fungi.","PeriodicalId":20803,"journal":{"name":"Rafidain journal of science","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76806435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-01DOI: 10.33899/rjs.2023.177286
Sanaa Ahmed, H. Khalaf, N. Jafar, B. Noomi
Toxoplasma gondii is one of the most common parasites among humans worldwide, as serological studies indicate that more than one third of the population of the world is infected with this parasite. This parasite is one of the main causes of abortion in pregnant women which mainly occur in the acute phase of infection and in early pregnancy. The present study aimed to detect toxoplasma among pregnant women and its relationship with some immunological markers. Fifty blood samples (5ml) were collected from aborted woman (within 15 days after abortion) and 10 blood samples collected from healthy woman as control. The serums were used to evaluate IL-8, IL-10, IL-17, and IFN-γ. The results showed that toxoplasma caused abortion in the first trimester in rate of 68%. The majority of aborted women (76%) were at age group 25-30 years. As for immunological parameters, IL-8, IL-10, IL-17, and IFN-γ significantly increased in woman aborted with toxoplasmosis in compare with healthy woman. The parameters were also higher among toxoplasma positive patients when compared with aborted women with other cause but these increases in the immunological parameters were not significant. The study conclude that toxoplasma is still the main causes of abortion in women and it associated with significant increase in immunological markers represented by IL-8, IL-10, IL-17, and IFN-γ.
{"title":"Estimation of the Levels of some Immunological Markers in Aborted Women Infected with Toxoplasma gondii at Baghdad City","authors":"Sanaa Ahmed, H. Khalaf, N. Jafar, B. Noomi","doi":"10.33899/rjs.2023.177286","DOIUrl":"https://doi.org/10.33899/rjs.2023.177286","url":null,"abstract":"Toxoplasma gondii is one of the most common parasites among humans worldwide, as serological studies indicate that more than one third of the population of the world is infected with this parasite. This parasite is one of the main causes of abortion in pregnant women which mainly occur in the acute phase of infection and in early pregnancy. The present study aimed to detect toxoplasma among pregnant women and its relationship with some immunological markers. Fifty blood samples (5ml) were collected from aborted woman (within 15 days after abortion) and 10 blood samples collected from healthy woman as control. The serums were used to evaluate IL-8, IL-10, IL-17, and IFN-γ. The results showed that toxoplasma caused abortion in the first trimester in rate of 68%. The majority of aborted women (76%) were at age group 25-30 years. As for immunological parameters, IL-8, IL-10, IL-17, and IFN-γ significantly increased in woman aborted with toxoplasmosis in compare with healthy woman. The parameters were also higher among toxoplasma positive patients when compared with aborted women with other cause but these increases in the immunological parameters were not significant. The study conclude that toxoplasma is still the main causes of abortion in women and it associated with significant increase in immunological markers represented by IL-8, IL-10, IL-17, and IFN-γ.","PeriodicalId":20803,"journal":{"name":"Rafidain journal of science","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86178206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-01DOI: 10.33899/rjs.2023.177296
R. Hassan, A. Al-Sabawi
This work includes the synthesis of some new pyrazole derivatives from the reaction of new - unsaturated carbonyl derivatives with hydrazine hydrate. - Unsaturated carbonyl derivatives which are prepared in several ways by reaction of hydrazide-hydrazone A 2 with substituted aromatic aldehydes in the presence of triethylamine as a base, and also prepared by reaction of hydrazide-hydrazone A 2 with phenylisothiocyanate and (ethyl chloro acetate or dimethyl sulfate) or A 2 with carbon disulfide and (ethyl chloro acetate or dimethyl sulfate) in the presence of potassium hydroxide in dry dimethyl formamide. The structures of these new synthesized compounds were confirmed by physical and spectroscopic methods (FT-IR, 1 H-NMR, 13 C-NMR)
{"title":"Synthesis and Spectral Study of some New α, β-Unsaturated Carbonyl Compounds and Pyrazole Derivatives","authors":"R. Hassan, A. Al-Sabawi","doi":"10.33899/rjs.2023.177296","DOIUrl":"https://doi.org/10.33899/rjs.2023.177296","url":null,"abstract":"This work includes the synthesis of some new pyrazole derivatives from the reaction of new - unsaturated carbonyl derivatives with hydrazine hydrate. - Unsaturated carbonyl derivatives which are prepared in several ways by reaction of hydrazide-hydrazone A 2 with substituted aromatic aldehydes in the presence of triethylamine as a base, and also prepared by reaction of hydrazide-hydrazone A 2 with phenylisothiocyanate and (ethyl chloro acetate or dimethyl sulfate) or A 2 with carbon disulfide and (ethyl chloro acetate or dimethyl sulfate) in the presence of potassium hydroxide in dry dimethyl formamide. The structures of these new synthesized compounds were confirmed by physical and spectroscopic methods (FT-IR, 1 H-NMR, 13 C-NMR)","PeriodicalId":20803,"journal":{"name":"Rafidain journal of science","volume":"219 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80096193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-01DOI: 10.33899/rjs.2023.177284
Maha Al-Taei, Badia Malla Obaeda
{"title":"Isolation and Identification of some Yeasts from some Plants","authors":"Maha Al-Taei, Badia Malla Obaeda","doi":"10.33899/rjs.2023.177284","DOIUrl":"https://doi.org/10.33899/rjs.2023.177284","url":null,"abstract":"","PeriodicalId":20803,"journal":{"name":"Rafidain journal of science","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84986749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-01DOI: 10.33899/rjs.2023.177292
Muhammed Z. Bakir, Khalid M. Ahmed, Ramadan Y. Mohamed
During spring of 2022, leaf scorch symptom was observed in Nerium oleander plant in Erbil city. Surveys were carried out in three main Erbil streets (60-meter, 120 meter and Qasmlo) to determine the occurrence and distribution of oleander leaf scorch. Oleander leaf scorch (OLS) is a disease caused by xylem limited bacterium Xylella fastidiosa . The percentage of plants with Oleander leaf scorch infection ranged usually between 23-91%, and the disease was assessed the highest disease rate based on disease rating scale was recorded in 60-meter street is 91.95%, Qasmlo street is 89.07% and the lowest disease rate was observed in 120-meter street is 23.17%. In this study the bacterium was detected after isolated in laboratory as Gram stain test was performed and the isolated bacterium was Gram negative, rod shaped, non-flagellate and non-motile also identified by PCR were performed with two primers forward primer 16S (AGAG TTTG ATCC TGGC TCAG) and reverse primer 16S (GGCT ACCT TGTT ACGA CTT), amplified a 1200 bp fragment from the isolated OLS. The Analysis of BLAST revealed that the highest identity number query sequences were (100%) identified and submitted in GenBank and have taken accession number Xylella fastidiosa subsp. Sandyi (ON131099) and Xylella fastidiosa subsp. Sandyi (ON131100) also the phylogenetic results show the each identified bacteria are grouped in one clade with other sequences were stored in NCBI GenBank. This is a first report of a „ Xylella fastidiosa .in Erbil city.
{"title":"Isolation and Identification of Xylella fastidiosa that Cause Oleander Leaf Scorch","authors":"Muhammed Z. Bakir, Khalid M. Ahmed, Ramadan Y. Mohamed","doi":"10.33899/rjs.2023.177292","DOIUrl":"https://doi.org/10.33899/rjs.2023.177292","url":null,"abstract":"During spring of 2022, leaf scorch symptom was observed in Nerium oleander plant in Erbil city. Surveys were carried out in three main Erbil streets (60-meter, 120 meter and Qasmlo) to determine the occurrence and distribution of oleander leaf scorch. Oleander leaf scorch (OLS) is a disease caused by xylem limited bacterium Xylella fastidiosa . The percentage of plants with Oleander leaf scorch infection ranged usually between 23-91%, and the disease was assessed the highest disease rate based on disease rating scale was recorded in 60-meter street is 91.95%, Qasmlo street is 89.07% and the lowest disease rate was observed in 120-meter street is 23.17%. In this study the bacterium was detected after isolated in laboratory as Gram stain test was performed and the isolated bacterium was Gram negative, rod shaped, non-flagellate and non-motile also identified by PCR were performed with two primers forward primer 16S (AGAG TTTG ATCC TGGC TCAG) and reverse primer 16S (GGCT ACCT TGTT ACGA CTT), amplified a 1200 bp fragment from the isolated OLS. The Analysis of BLAST revealed that the highest identity number query sequences were (100%) identified and submitted in GenBank and have taken accession number Xylella fastidiosa subsp. Sandyi (ON131099) and Xylella fastidiosa subsp. Sandyi (ON131100) also the phylogenetic results show the each identified bacteria are grouped in one clade with other sequences were stored in NCBI GenBank. This is a first report of a „ Xylella fastidiosa .in Erbil city.","PeriodicalId":20803,"journal":{"name":"Rafidain journal of science","volume":"97 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87227119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-01DOI: 10.33899/rjs.2023.177283
Z. Qaddawi, A. Mohammed, William O. Onu
Microbacterium sp. AJ-Z was isolated from fenugreek ( Trigonella foenum-graecum ) root nodules on Yeast Extract Mannitol (YEM) medium. Colonies of the bacteria isolates grown were rod shape, whitish, translucent, sticky nature. The results showed responded of the fenugreek seedlings grown on Nitrogen free medium (NF) to inoculated with Microbacterium sp. AJ-Z isolate at 30 min. and successful to form nodules its roots in the rate of 70% after 7 days with rate number of nodes/seedling was 2.9 superiority on the other which grown on Murashige and Skoog (MS) medium and 0.7% agar. Nodules produced have a spherical shape, pinkish and indeterminate in growth and after a few days, its shape becomes elongated.
{"title":"Detection of Family Specialty for Microbacterium sp. AJ-Z Isolated from Fenugreek (Trigonella foenum-graecum) Root Nodules","authors":"Z. Qaddawi, A. Mohammed, William O. Onu","doi":"10.33899/rjs.2023.177283","DOIUrl":"https://doi.org/10.33899/rjs.2023.177283","url":null,"abstract":"Microbacterium sp. AJ-Z was isolated from fenugreek ( Trigonella foenum-graecum ) root nodules on Yeast Extract Mannitol (YEM) medium. Colonies of the bacteria isolates grown were rod shape, whitish, translucent, sticky nature. The results showed responded of the fenugreek seedlings grown on Nitrogen free medium (NF) to inoculated with Microbacterium sp. AJ-Z isolate at 30 min. and successful to form nodules its roots in the rate of 70% after 7 days with rate number of nodes/seedling was 2.9 superiority on the other which grown on Murashige and Skoog (MS) medium and 0.7% agar. Nodules produced have a spherical shape, pinkish and indeterminate in growth and after a few days, its shape becomes elongated.","PeriodicalId":20803,"journal":{"name":"Rafidain journal of science","volume":"91 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79488050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-01DOI: 10.33899/rjs.2023.177293
Muhammed O. Muhammed
Starting from benzimidazole moiety, several heterocyclic compounds (1,3-Oxazepine, thiazolidin-4-one, azetidin-2-one) were synthesized, thus the Ethyl Benzimidazole acetate (1) was synthesized from the reaction of benzimidazole with ethylchloroacetate on treatment with hydrazine hydrate is giving the corresponding hydrazide (2). The reaction of this hydrazide with, 4-substituted acetophenone afforded hydrazones which on reaction with some reagents to produce synthesized compounds, (4a-c), (5a-c), (6a-c), (7a-c), (8a-c) and (9a-c). All the synthesized products were confirmed by physical and spectral methods.
{"title":"Synthesis, Characterization of Substituted 1,3-Oxazepine, Thiazolidine-4-one and Azetidine-2-one Using Benzimidazole as a Synthon","authors":"Muhammed O. Muhammed","doi":"10.33899/rjs.2023.177293","DOIUrl":"https://doi.org/10.33899/rjs.2023.177293","url":null,"abstract":"Starting from benzimidazole moiety, several heterocyclic compounds (1,3-Oxazepine, thiazolidin-4-one, azetidin-2-one) were synthesized, thus the Ethyl Benzimidazole acetate (1) was synthesized from the reaction of benzimidazole with ethylchloroacetate on treatment with hydrazine hydrate is giving the corresponding hydrazide (2). The reaction of this hydrazide with, 4-substituted acetophenone afforded hydrazones which on reaction with some reagents to produce synthesized compounds, (4a-c), (5a-c), (6a-c), (7a-c), (8a-c) and (9a-c). All the synthesized products were confirmed by physical and spectral methods.","PeriodicalId":20803,"journal":{"name":"Rafidain journal of science","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91426117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-01DOI: 10.33899/rjs.2023.177290
Sawsan J. A. AL-Allaf, Rehab Albaker
{"title":"Green Synthesis of Silver Nanoparticles Using Nigella sativa Callus and Cellular Suspension Cultures with Different pH Values","authors":"Sawsan J. A. AL-Allaf, Rehab Albaker","doi":"10.33899/rjs.2023.177290","DOIUrl":"https://doi.org/10.33899/rjs.2023.177290","url":null,"abstract":"","PeriodicalId":20803,"journal":{"name":"Rafidain journal of science","volume":"227 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76973913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-01DOI: 10.33899/rjs.2023.177291
Noor Al-healy, Warka Qassim
{"title":"Biodiversity of some Species of Alternaria Fungi Causing Spotting in Ornamental Plants","authors":"Noor Al-healy, Warka Qassim","doi":"10.33899/rjs.2023.177291","DOIUrl":"https://doi.org/10.33899/rjs.2023.177291","url":null,"abstract":"","PeriodicalId":20803,"journal":{"name":"Rafidain journal of science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90040325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.33899/rjs.2023.178571
E. G, E. I., Oparaji H.
The need for alternative and environmental friendly methods of waste clean-up has led to the use of enzymes in bioremediation. In this study, white rot fungi were isolated from decaying plant parts using standard microbiology and biochemical techniques. The isolated fungal mycelial were identified and screened using standard substrates (2, 6-DMP) to determine their capability for production of Manganese peroxidase. Pure manganese peroxidase was achieved after four distinct purification phases. Crude extract of the homogenate proteins obtained through optimized submerged fermentation system were precipitated using ammonium sulphate salt. Ammonium sulphate of 60% concentrated at pH 4.5 precipitated protein with highest Manganese peroxidase activity (322 U/mg). The precipitated proteins was desalted through dialysis for twelve hours (12hrs) with buffer exchange at interval of six hours (6 hrs) and activity of 343.91 U/mg was recorded afterwards. DEAE-cellulose was used for the ion exchange purification of the dialyzed protein, salt (NaCl) gradients of 0.3-0.6 M was found best in washing off the bound protein from the exchange resin and activity of 434.18 U/mg was recorded from the pooled fraction tubes. sephadex G-100 was used for separation of the proteins into molecular sizes and weights. 2.8 purification folds of the enzyme were gotten after ion exchange (DEAE-cellulose) and gel filtration (sephadex G-100) with percentage yield of 2.20%. Specific activity of the enzyme increased to 602.00% after gel filtration. The partial purified enzyme was further characterized for determination of optimal pH (4.5), temperature (40 °C) and kinetic properties K M of 3.4 mM and V MAX of 250 µmol/min.
由于需要替代和环境友好的废物清理方法，因此在生物修复中使用酶。本研究采用标准的微生物学和生化技术从腐烂的植物中分离出白腐菌。利用标准底物(2,6 - dmp)对分离的真菌菌丝体进行鉴定和筛选，以确定其生产过氧化物锰酶的能力。经过四个不同的纯化阶段，获得了纯锰过氧化物酶。将优化后的深层发酵体系得到的蛋白匀浆粗提物用硫酸铵进行沉淀。浓度为60%，pH为4.5的硫酸铵沉淀蛋白，锰过氧化物酶活性最高(322 U/mg)。沉淀蛋白经透析12小时(12hrs)脱盐，每隔6小时(6小时)交换缓冲液，测得活性为343.91 U/mg。采用deae -纤维素对透析蛋白进行离子交换纯化，在0.3 ~ 0.6 M的盐梯度范围内洗涤效果最好，池馏管的活性为434.18 U/mg。sephadex G-100用于分离蛋白质的分子量和分子量。经离子交换(DEAE-cellulose)和凝胶过滤(sephadex G-100)得到2.8个纯化褶，得率为2.20%。经凝胶过滤后，酶的比活性提高到602.00%。对部分纯化酶进行了进一步表征，确定了最佳pH(4.5)、温度(40°C)和动力学性质K M为3.4 mM, V MAX为250µmol/min。
{"title":"Screening of Conditions for Manganese Peroxidase Production from White Rot Fungi (Pleurotus djamor)","authors":"E. G, E. I., Oparaji H.","doi":"10.33899/rjs.2023.178571","DOIUrl":"https://doi.org/10.33899/rjs.2023.178571","url":null,"abstract":"The need for alternative and environmental friendly methods of waste clean-up has led to the use of enzymes in bioremediation. In this study, white rot fungi were isolated from decaying plant parts using standard microbiology and biochemical techniques. The isolated fungal mycelial were identified and screened using standard substrates (2, 6-DMP) to determine their capability for production of Manganese peroxidase. Pure manganese peroxidase was achieved after four distinct purification phases. Crude extract of the homogenate proteins obtained through optimized submerged fermentation system were precipitated using ammonium sulphate salt. Ammonium sulphate of 60% concentrated at pH 4.5 precipitated protein with highest Manganese peroxidase activity (322 U/mg). The precipitated proteins was desalted through dialysis for twelve hours (12hrs) with buffer exchange at interval of six hours (6 hrs) and activity of 343.91 U/mg was recorded afterwards. DEAE-cellulose was used for the ion exchange purification of the dialyzed protein, salt (NaCl) gradients of 0.3-0.6 M was found best in washing off the bound protein from the exchange resin and activity of 434.18 U/mg was recorded from the pooled fraction tubes. sephadex G-100 was used for separation of the proteins into molecular sizes and weights. 2.8 purification folds of the enzyme were gotten after ion exchange (DEAE-cellulose) and gel filtration (sephadex G-100) with percentage yield of 2.20%. Specific activity of the enzyme increased to 602.00% after gel filtration. The partial purified enzyme was further characterized for determination of optimal pH (4.5), temperature (40 °C) and kinetic properties K M of 3.4 mM and V MAX of 250 µmol/min.","PeriodicalId":20803,"journal":{"name":"Rafidain journal of science","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77254473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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