Pub Date : 2024-05-23DOI: 10.31830/2348-7542.2024.roc-1018
GAVIN GELDENHUYS
Plant research has recently concentrated on the molecular level, which requires reliable, inexpensive, and fast protocols for extracting DNA. Many researchers have identified Chelex-based DNA extraction methods as having the potential to reduce costs. However, Chelex can also prevent DNA degradation by chelating metal ions. Citrus is one of the top three crops grown globally, with oranges being a well-known species. Therefore, study on the molecular of C. sinensis is essential. This study was conducted in 2022 at the genetic lab of the University of Limpopo- to compare two different extraction methods for DNA from C. sinensis. The DNA from the C. sinensis leaves was extracted using Chelex "method 1" (overnight incubation at 56°C) and "method 2" (Ten-minute incubation at 95°C). A spectrophotometer was employed to measure the extracted DNA's qualitative and quantitative parameters accurately. Furthermore, all samples were subjected to PCR reactions with primers for 28S. The results indicated that "method 2" (1.59-1.84) proved to be the most effective method to obtain high-quality extracted DNA. Additionally, the quantitative and qualitative tests for PCR reaction demonstrated that "method 2" produced better results. In conclusion, although both methods yielded favourable outcomes, "method 2" was ultimately the more successful approach.
植物研究最近集中在分子水平，这就需要可靠、廉价和快速的 DNA 提取方案。许多研究人员发现，基于 Chelex 的 DNA 提取方法具有降低成本的潜力。不过，Chelex 还能通过螯合金属离子防止 DNA 降解。柑橘是全球三大作物之一，其中橙子是著名的品种。因此，对柑橘分子的研究至关重要。这项研究于 2022 年在林波波大学的基因实验室进行，目的是比较从中华橘中提取 DNA 的两种不同方法。采用Chelex "方法1"（56°C过夜孵育）和 "方法2"（95°C十分钟孵育）从中华皂苷叶片中提取DNA。使用分光光度计精确测量提取 DNA 的定性和定量参数。此外，所有样品都用 28S 引物进行了 PCR 反应。结果表明，"方法 2"（1.59-1.84）是获得高质量提取 DNA 的最有效方法。此外，PCR 反应的定量和定性测试表明，"方法 2 "的结果更好。总之，虽然两种方法都取得了良好的结果，但 "方法 2 "最终是更成功的方法。
{"title":"Evaluating the efficiency of Chelex 100 for DNA extraction in orange (Citrus sinensis)","authors":"GAVIN GELDENHUYS","doi":"10.31830/2348-7542.2024.roc-1018","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1018","url":null,"abstract":"Plant research has recently concentrated on the molecular level, which requires reliable, inexpensive, and fast protocols for extracting DNA. Many researchers have identified Chelex-based DNA extraction methods as having the potential to reduce costs. However, Chelex can also prevent DNA degradation by chelating metal ions. Citrus is one of the top three crops grown globally, with oranges being a well-known species. Therefore, study on the molecular of C. sinensis is essential. This study was conducted in 2022 at the genetic lab of the University of Limpopo- to compare two different extraction methods for DNA from C. sinensis. The DNA from the C. sinensis leaves was extracted using Chelex \"method 1\" (overnight incubation at 56°C) and \"method 2\" (Ten-minute incubation at 95°C). A spectrophotometer was employed to measure the extracted DNA's qualitative and quantitative parameters accurately. Furthermore, all samples were subjected to PCR reactions with primers for 28S. The results indicated that \"method 2\" (1.59-1.84) proved to be the most effective method to obtain high-quality extracted DNA. Additionally, the quantitative and qualitative tests for PCR reaction demonstrated that \"method 2\" produced better results. In conclusion, although both methods yielded favourable outcomes, \"method 2\" was ultimately the more successful approach.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"51 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141107298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-23DOI: 10.31830/2348-7542.2024.roc-1058
Cucu Suherman, Jajang Supriatna, A. Nuraini, Syariful Mubarok
The essential role of fertilization in the management of oil palm plantations cannot be overstated, with the understanding that effective strategies must be fine-tuned to accommodate the diversity of soil types present. The nuanced response of oil palm to fertilization under different soil conditions underscores the critical nature of this research. This study investigates the relationship between oil palm farming practices and agro-environmental performance of plantations. This research aims to understand the complex mechanisms influencing plantation productivity. Five agronomic field experiments were carried out on various soil types (Podzol, Inceptisol, Peat) during 2022. These experiments involved various types of soil and used different plant materials in a total land area of 33 hectares. The results of the peat soil trials revealed a positive effect of RP fertilizer on leaf phosphorus (P) content and a visible gradient in potassium (K) content with different doses of KCl. Average plantation yields peaked in 2022, showing the best yields ever recorded. On the contrary, experiments in podzolic soils showed that oil palm responded better to organic fertilizers than to mineral fertilizers in podzolic soils. The inceptisol soil revealed a significant increase in leaf potassium (K) content with KCl dose, confirming the positive effect of fresh oil palm empty fruit bunches (EFB) on potassium levels. In particular, EFB acts as a good K fertilizer, positively influencing the K content but negatively affecting the calcium content (Ca) due to K/Ca antagonism. These findings emphasize the need for diversified agricultural strategies to optimize oil palm cultivation, increase productivity, and reduce dependence on external input.
{"title":"Optimizing oil palm farming: Soil quality, fertilization and agro-environmental performance","authors":"Cucu Suherman, Jajang Supriatna, A. Nuraini, Syariful Mubarok","doi":"10.31830/2348-7542.2024.roc-1058","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1058","url":null,"abstract":"\u0000The essential role of fertilization in the management of oil palm plantations cannot be overstated, with the understanding that effective strategies must be fine-tuned to accommodate the diversity of soil types present. The nuanced response of oil palm to fertilization under different soil conditions underscores the critical nature of this research. This study investigates the relationship between oil palm farming practices and agro-environmental performance of plantations. This research aims to understand the complex mechanisms influencing plantation productivity. Five agronomic field experiments were carried out on various soil types (Podzol, Inceptisol, Peat) during 2022. These experiments involved various types of soil and used different plant materials in a total land area of 33 hectares. The results of the peat soil trials revealed a positive effect of RP fertilizer on leaf phosphorus (P) content and a visible gradient in potassium (K) content with different doses of KCl. Average plantation yields peaked in 2022, showing the best yields ever recorded. On the contrary, experiments in podzolic soils showed that oil palm responded better to organic fertilizers than to mineral fertilizers in podzolic soils. The inceptisol soil revealed a significant increase in leaf potassium (K) content with KCl dose, confirming the positive effect of fresh oil palm empty fruit bunches (EFB) on potassium levels. In particular, EFB acts as a good K fertilizer, positively influencing the K content but negatively affecting the calcium content (Ca) due to K/Ca antagonism. These findings emphasize the need for diversified agricultural strategies to optimize oil palm cultivation, increase productivity, and reduce dependence on external input.","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"24 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141106231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-23DOI: 10.31830/2348-7542.2024.roc-1079
SAGAR MAITRA, Masina Sairam, D. T. SANTOSH, DINKAR JAGANNATH GAIKWAD, Upasana Sahoo
In developing countries like India, rapid urbanisation was under way and the diet habits of the people have been changing day to day. Under this transformation, a section of the population is focused on nutritional security where vegetables play a major role in daily diet. Among different vegetables coloured bell pepper which is commonly known as capsicum, has importance due to its nutritional quality and market demand. The quality and productivity of coloured capsicum can be improved by choosing the best performing hybrids and exogenous application of plant growth regulators. Considering the above, the present study was conducted from November to April of 2020-21 and 2021-22 at the Protected Cultivation Unit of Centurion University of Technology and Management, Paralakhemundi, Odisha under controlled environment conditions. The experiment was laid out in factorial randomized block design consisting of two hybrids, namely, Orobelle (yellow colored) and Bomby (red colored); and eight treatments of growth regulators which were GA3 @ 25 ppm, GA3 @ 50 ppm, GA3 @ 75 ppm, GA3 @ 100 ppm, NAA @ 20 ppm, NAA @ 40 ppm, NAA @ 60 ppm and NAA @ 80 ppm. A total of 16 treatment combinations were worked out in the experiment which were replicated thrice. The results of the experiment revealed that there was no significant variation among the hybrids cultivated with respect to yield attributes, yield, quality parameters and growth attributes excluding plant height and number of leaves per plant at 60 DAS and 90 DAS. However, there was a significant impact of plant growth regulators on growth attributes, yield attributes, yield and quality parameters. The application of NAA @ 80 ppm resulted in early flowering (43.8 days after planting). Moreover, the maximum number of flowers per plant (32.5 flowers/plant), flower to fruit ratio (52.9), fruits per plant (19.5) and weight of the fruit (133.6 g) recorded in GA3 @ 100 ppm application. There was a significant interaction effect among treatment combinations in fruit yield where the highest value was recorded in Bomby hybrid with GA3 @ 100 PPM application. From the study it may be concluded that cultivation of both the hybrids can be considered as per the fruit colour and market demand along with the application of GA3 @ 100 ppm under controlled environment conditions.
{"title":"Growth, productivity and quality of colored capsicum (Capsicum annuum L.) as influenced by hybrids and plant growth regulators under controlled environment conditions ","authors":"SAGAR MAITRA, Masina Sairam, D. T. SANTOSH, DINKAR JAGANNATH GAIKWAD, Upasana Sahoo","doi":"10.31830/2348-7542.2024.roc-1079","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1079","url":null,"abstract":"In developing countries like India, rapid urbanisation was under way and the diet habits of the people have been changing day to day. Under this transformation, a section of the population is focused on nutritional security where vegetables play a major role in daily diet. Among different vegetables coloured bell pepper which is commonly known as capsicum, has importance due to its nutritional quality and market demand. The quality and productivity of coloured capsicum can be improved by choosing the best performing hybrids and exogenous application of plant growth regulators. Considering the above, the present study was conducted from November to April of 2020-21 and 2021-22 at the Protected Cultivation Unit of Centurion University of Technology and Management, Paralakhemundi, Odisha under controlled environment conditions. The experiment was laid out in factorial randomized block design consisting of two hybrids, namely, Orobelle (yellow colored) and Bomby (red colored); and eight treatments of growth regulators which were GA3 @ 25 ppm, GA3 @ 50 ppm, GA3 @ 75 ppm, GA3 @ 100 ppm, NAA @ 20 ppm, NAA @ 40 ppm, NAA @ 60 ppm and NAA @ 80 ppm. A total of 16 treatment combinations were worked out in the experiment which were replicated thrice. The results of the experiment revealed that there was no significant variation among the hybrids cultivated with respect to yield attributes, yield, quality parameters and growth attributes excluding plant height and number of leaves per plant at 60 DAS and 90 DAS. However, there was a significant impact of plant growth regulators on growth attributes, yield attributes, yield and quality parameters. The application of NAA @ 80 ppm resulted in early flowering (43.8 days after planting). Moreover, the maximum number of flowers per plant (32.5 flowers/plant), flower to fruit ratio (52.9), fruits per plant (19.5) and weight of the fruit (133.6 g) recorded in GA3 @ 100 ppm application. There was a significant interaction effect among treatment combinations in fruit yield where the highest value was recorded in Bomby hybrid with GA3 @ 100 PPM application. From the study it may be concluded that cultivation of both the hybrids can be considered as per the fruit colour and market demand along with the application of GA3 @ 100 ppm under controlled environment conditions.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"22 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141105246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-23DOI: 10.31830/2348-7542.2024.roc-1063
Maropeng Choshi, Yvonne Maila
The nutritive mineral element concentrations of most African leafy vegetables (ALVs) are influenced by the plant’s growth stages. Momordica balsamina is an ALV in the Cucurbitaceae family, rich with nutraceutical and pharmaceutical properties, available in the plant at different growth stages. A greenhouse study was conducted to determine the responses of the nutritional value of M. balsamina leafy vegetable harvested at different growth stages. Six growth stages, namely, vegetative (reference), bud development, flower initiation, fruit set, fruit development and physiological maturity, served as treatments, with 10 replicates and arranged in RCBD. Treatments had highly significant (P ≤ 0.01) effect on the tested nutritional quality, namely, potassium (K), calcium (Ca), magnesium (Mg), zinc (Zn), iron (Fe) and phosphorus (P). Relative to the reference, K was the highest (1206 mg/L) at flower initiation stage, whereas the highest Ca (127.70 mg/L) and Zn (2.21 mg/L) concentrations occurred at the fruit development stage. At the physiological maturity stage, the highest Mg (25.19 mg/L), P (16.54 mg/L) and Fe (5.49 mg/L) concentrations, were recorded. Noticeably, during early growth stages, P, Fe and Zn were negatively reduced, when compared to K, Ca and Mg, which were increasing at every growth stages. In conclusion, the greatest accumulation of the tested mineral elements was mainly observed as from when the leafy vegetable started setting fruit until physiological maturity stage.
{"title":"Influence of growth stages on the nutritional value of Momordica balsamina leaf powder ","authors":"Maropeng Choshi, Yvonne Maila","doi":"10.31830/2348-7542.2024.roc-1063","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1063","url":null,"abstract":"The nutritive mineral element concentrations of most African leafy vegetables (ALVs) are influenced by the plant’s growth stages. Momordica balsamina is an ALV in the Cucurbitaceae family, rich with nutraceutical and pharmaceutical properties, available in the plant at different growth stages. A greenhouse study was conducted to determine the responses of the nutritional value of M. balsamina leafy vegetable harvested at different growth stages. Six growth stages, namely, vegetative (reference), bud development, flower initiation, fruit set, fruit development and physiological maturity, served as treatments, with 10 replicates and arranged in RCBD. Treatments had highly significant (P ≤ 0.01) effect on the tested nutritional quality, namely, potassium (K), calcium (Ca), magnesium (Mg), zinc (Zn), iron (Fe) and phosphorus (P). Relative to the reference, K was the highest (1206 mg/L) at flower initiation stage, whereas the highest Ca (127.70 mg/L) and Zn (2.21 mg/L) concentrations occurred at the fruit development stage. At the physiological maturity stage, the highest Mg (25.19 mg/L), P (16.54 mg/L) and Fe (5.49 mg/L) concentrations, were recorded. Noticeably, during early growth stages, P, Fe and Zn were negatively reduced, when compared to K, Ca and Mg, which were increasing at every growth stages. In conclusion, the greatest accumulation of the tested mineral elements was mainly observed as from when the leafy vegetable started setting fruit until physiological maturity stage.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"20 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141105255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-23DOI: 10.31830/2348-7542.2024.roc-1017
GAVIN GELDENHUYS
Tomato (Solanum lycopersicum L.) is one of the premier vegetables in the world and is widely cultivated globally. As it is a relatively short-duration crop with a high yield, it is economically attractive, and the area under cultivation is increasing. Plant-parasitic nematodes are some of the most important plant pathogens in tomato farms. Helicotylenchus pseudorobustus is one of the most important plant-parasitic nematodes in tomato fields. Therefore, understanding the population dynamics of H. pseudorobustus on the tomato field assists the farmer in managing them and having better yields. In this study, the soil was sampled in 2022. Identification and determination of nematodes were made in the Aquaculture Research Unit, University of Limpopo. The properties of soils infected with this nematode, including pH and EC, were investigated during the investigation of this nematode in the Dalmatia region. The population of H. pseudorobustus was counted in 100 grams of soil in each season, and soil characteristics were also analyzed at the same time. The results in different seasons of the year showed that the range of pH changes during the years of investigation was between 7.5 and 7.7, and the range of EC changes was between 1.3 and 2.0 dS/m. A comparison of EC and pH changes with population changes showed that the population increased approximately in the months when soil salinity decreased. In addition, as the pH increases, the population of H. pseudorobustus also increases. However, no significant relationship was found between the values of pH, EC, and changes in the population of H. pseudorobustus nematode throughout the year.
{"title":"Investigation on the population dynamics of Helicotylenchus pseudorobustus and its relationship with soil factors in tomato fields","authors":"GAVIN GELDENHUYS","doi":"10.31830/2348-7542.2024.roc-1017","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1017","url":null,"abstract":"Tomato (Solanum lycopersicum L.) is one of the premier vegetables in the world and is widely cultivated globally. As it is a relatively short-duration crop with a high yield, it is economically attractive, and the area under cultivation is increasing. Plant-parasitic nematodes are some of the most important plant pathogens in tomato farms. Helicotylenchus pseudorobustus is one of the most important plant-parasitic nematodes in tomato fields. Therefore, understanding the population dynamics of H. pseudorobustus on the tomato field assists the farmer in managing them and having better yields. In this study, the soil was sampled in 2022. Identification and determination of nematodes were made in the Aquaculture Research Unit, University of Limpopo. The properties of soils infected with this nematode, including pH and EC, were investigated during the investigation of this nematode in the Dalmatia region. The population of H. pseudorobustus was counted in 100 grams of soil in each season, and soil characteristics were also analyzed at the same time. The results in different seasons of the year showed that the range of pH changes during the years of investigation was between 7.5 and 7.7, and the range of EC changes was between 1.3 and 2.0 dS/m. A comparison of EC and pH changes with population changes showed that the population increased approximately in the months when soil salinity decreased. In addition, as the pH increases, the population of H. pseudorobustus also increases. However, no significant relationship was found between the values of pH, EC, and changes in the population of H. pseudorobustus nematode throughout the year.","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"53 50","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141102936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-23DOI: 10.31830/2348-7542.2024.roc-1077
T. N. LE, D. D. Nguyen, D. T. Nguyen
The expansion of agricultural production and urbanization has led to the depletion of the global forest ecosystem, resulting in land cover changes (LCCs) that pose threats to the land environment. Therefore, it is imperative to identify these LCCs as an essential step toward resolution and mitigation. This study aims to evaluate temporal LCCs in Thai Nguyen province by utilizing GIS and remote sensing techniques (RST) to analyze high-resolution satellite imagery spanning from 2001 to 2023. LCCs were identified using semi-automatic classification plugin (SCP) techniques with Landsat-8 and Sentinel-2 images in ERVI software. The accuracy of the LCC maps was validated through post-classification comparisons, yielding high precision rates ranging from 87% to 96%. The results indicate a significant reduction in forest cover area, declining by 1018 ha from 226,18 ha in 2001 to 207,22 ha in 2023. This decline primarily occurred in urban areas, highlighting the intensification of urbanization processes. Cultivated and bare land experienced a minor decrease and a substantial decrease from 18,631 ha and 105,000 ha to 16,578 ha and 86,82 ha between 2001 and 2010, followed by a slight increase and a significant increase to 5,069 ha and 24,487 ha (1.4% and 6.9%) during the 2010-2023 period. These findings underscore the escalating risk of diminishing forest cover in the study area.
{"title":"Land cover change assessment in Thai Nguyen Province, Vietnam using GIS and remote sensing techniques ","authors":"T. N. LE, D. D. Nguyen, D. T. Nguyen","doi":"10.31830/2348-7542.2024.roc-1077","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1077","url":null,"abstract":"The expansion of agricultural production and urbanization has led to the depletion of the global forest ecosystem, resulting in land cover changes (LCCs) that pose threats to the land environment. Therefore, it is imperative to identify these LCCs as an essential step toward resolution and mitigation. This study aims to evaluate temporal LCCs in Thai Nguyen province by utilizing GIS and remote sensing techniques (RST) to analyze high-resolution satellite imagery spanning from 2001 to 2023. LCCs were identified using semi-automatic classification plugin (SCP) techniques with Landsat-8 and Sentinel-2 images in ERVI software. The accuracy of the LCC maps was validated through post-classification comparisons, yielding high precision rates ranging from 87% to 96%. The results indicate a significant reduction in forest cover area, declining by 1018 ha from 226,18 ha in 2001 to 207,22 ha in 2023. This decline primarily occurred in urban areas, highlighting the intensification of urbanization processes. Cultivated and bare land experienced a minor decrease and a substantial decrease from 18,631 ha and 105,000 ha to 16,578 ha and 86,82 ha between 2001 and 2010, followed by a slight increase and a significant increase to 5,069 ha and 24,487 ha (1.4% and 6.9%) during the 2010-2023 period. These findings underscore the escalating risk of diminishing forest cover in the study area.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"8 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141104703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-22DOI: 10.31830/2348-7542.2024.roc-1075
T. D. Phung
This study aims to assess the impacts of land use change (LUC) on soil properties and soil organic carbon content (SOCC) in the mountainous regions of Nghe An province, Vietnam. Soil samples were surveyed from forest plant area (FPA), annual plant area (APA), crop plant area (CPA), and bare hill area (BHA) at depths ranging from 0-50 cm during the period 2021-2023. The findings indicated that the conversion from FPA to BHA resulted in a significant decline in SOCC. In comparison to the topsoil layer, reductions of approximately 18.6-22.1% for FPA, 26.5-36.0% for CPA and APA, and up to 37.5% for BHA were observed in the soil profiles. These results underscore the sensitivity of SOCC to LUC across the study area. Moreover, the study contributes to our understanding of the implications of LUC on soil health and offers valuable insights for effective land management and conservation strategies. The conversion of FPA into various types of land use (TLU) has a detrimental impact on soil properties, highlighting the urgent necessity for sustainable land use practices.
{"title":"Assessing the impact of unsustainable exploitation and climate change on soil properties alterations in the mountainous regions, Vietnam","authors":"T. D. Phung","doi":"10.31830/2348-7542.2024.roc-1075","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1075","url":null,"abstract":"This study aims to assess the impacts of land use change (LUC) on soil properties and soil organic carbon content (SOCC) in the mountainous regions of Nghe An province, Vietnam. Soil samples were surveyed from forest plant area (FPA), annual plant area (APA), crop plant area (CPA), and bare hill area (BHA) at depths ranging from 0-50 cm during the period 2021-2023. The findings indicated that the conversion from FPA to BHA resulted in a significant decline in SOCC. In comparison to the topsoil layer, reductions of approximately 18.6-22.1% for FPA, 26.5-36.0% for CPA and APA, and up to 37.5% for BHA were observed in the soil profiles. These results underscore the sensitivity of SOCC to LUC across the study area. Moreover, the study contributes to our understanding of the implications of LUC on soil health and offers valuable insights for effective land management and conservation strategies. The conversion of FPA into various types of land use (TLU) has a detrimental impact on soil properties, highlighting the urgent necessity for sustainable land use practices.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"57 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141108511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-22DOI: 10.31830/2348-7542.2024.roc-1071
Mehrnoush Aminisarteshnizi
The genus Pandoraea comprises gram-negative, motile, non-spore-forming, rod-shaped, oxidase-positive, and obligate aerobes bacteria with a single polar flagellum. Several species belonging to this genus have been identified as potentially risky pathogenic bacteria, which can adversely impact the yield of white cabbage plants. A study was conducted at the University of Limpopo in 2023 to isolate and identify the Pandoraea bacterium from a white cabbage field and a free-living nematode, Acrobeles complexus, in South Africa. The molecular study used the 16S rDNA marker to identify the bacterium associated with nematode and white cabbage fields from South Africa's soils. The DNA was extracted using the Chelex method, and the bacterium was identified as Pandoraea. Specific primers were used to amplify the 16S rDNA to identify the nematode. The Nblast analysis showed that South African Pandoraea has a 99% similarity with a population from India (AB510957). Phylogenetic analysis using maximum likelihood placed this species with those molecularly identified as Pandoraea in the same clade with highly supported (100) bootstrap values. In conclusion, this study has demonstrated that the 16S rDNA marker can be effectively used to identify Pandoraea species. However, the use of additional DNA markers is recommended to achieve a better understanding of Pandoraea's phylogeny.
{"title":" Analysing Pandoraea associated with Brassica oleracea using 16S rDNA from Limpopo Province, South Africa","authors":"Mehrnoush Aminisarteshnizi","doi":"10.31830/2348-7542.2024.roc-1071","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1071","url":null,"abstract":"The genus Pandoraea comprises gram-negative, motile, non-spore-forming, rod-shaped, oxidase-positive, and obligate aerobes bacteria with a single polar flagellum. Several species belonging to this genus have been identified as potentially risky pathogenic bacteria, which can adversely impact the yield of white cabbage plants. A study was conducted at the University of Limpopo in 2023 to isolate and identify the Pandoraea bacterium from a white cabbage field and a free-living nematode, Acrobeles complexus, in South Africa. The molecular study used the 16S rDNA marker to identify the bacterium associated with nematode and white cabbage fields from South Africa's soils. The DNA was extracted using the Chelex method, and the bacterium was identified as Pandoraea. Specific primers were used to amplify the 16S rDNA to identify the nematode. The Nblast analysis showed that South African Pandoraea has a 99% similarity with a population from India (AB510957). Phylogenetic analysis using maximum likelihood placed this species with those molecularly identified as Pandoraea in the same clade with highly supported (100) bootstrap values. In conclusion, this study has demonstrated that the 16S rDNA marker can be effectively used to identify Pandoraea species. However, the use of additional DNA markers is recommended to achieve a better understanding of Pandoraea's phylogeny.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"58 46","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141108436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-22DOI: 10.31830/2348-7542.2024.roc-1062
Naseer Faraj Shachai, Ahmed Ali Hussein, B. A. Abraheem
Food security is a major challenge faced by countries around the world due to the large increases in population numbers, climate change, and the problems it causes in agricultural production, such as droughts, floods, and hurricanes. Most oat varieties are characterized by their long stems, and this makes them effective Sink for the deposition of dry matter that can be directed to the grain in the case of the use of growth inhibitors (such as Cycocel and ethylene) that suppress the longitudinal growth of the stems and increase its diameter, as well as increase the number of effective branches, reduces the risk of lodging, thus increasing the grain yield. This experiment was conducted in the fields of the College of Agricultural Engineering Sciences, University of Baghdad, during the winter season of 2020-2021, to assess the effects of growth inhibitors Cycocel )CCC( and calcium carbide CaC2 (which generates ethylene), used three replications of a randomized complete block design (RCBD), Eight treatments were tested, including spraying CCC at concentrations of 200, 400, and 600 mg/L and ground application of CaC2 at 2 g/plant individually and in combination. The findings revealed that all treatments led to an increase in grain yield and a decrease in plant height compared to the control treatment. Notably, the C600 + CaC2 treatment exhibited significant superiority over other treatments in grain yield (5.52 t/ha), total dry matter (20.14 t/ha), and Harvest Index (25.21). These results suggest that growth inhibitors can effectively modulate the source-sink relationship, thereby enhancing grain yield.
{"title":"Investigating the influence of growth inhibitors on the growth and yield of oat (Avena sativa)","authors":"Naseer Faraj Shachai, Ahmed Ali Hussein, B. A. Abraheem","doi":"10.31830/2348-7542.2024.roc-1062","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1062","url":null,"abstract":"Food security is a major challenge faced by countries around the world due to the large increases in population numbers, climate change, and the problems it causes in agricultural production, such as droughts, floods, and hurricanes. Most oat varieties are characterized by their long stems, and this makes them effective Sink for the deposition of dry matter that can be directed to the grain in the case of the use of growth inhibitors (such as Cycocel and ethylene) that suppress the longitudinal growth of the stems and increase its diameter, as well as increase the number of effective branches, reduces the risk of lodging, thus increasing the grain yield. This experiment was conducted in the fields of the College of Agricultural Engineering Sciences, University of Baghdad, during the winter season of 2020-2021, to assess the effects of growth inhibitors Cycocel )CCC( and calcium carbide CaC2 (which generates ethylene), used three replications of a randomized complete block design (RCBD), Eight treatments were tested, including spraying CCC at concentrations of 200, 400, and 600 mg/L and ground application of CaC2 at 2 g/plant individually and in combination. The findings revealed that all treatments led to an increase in grain yield and a decrease in plant height compared to the control treatment. Notably, the C600 + CaC2 treatment exhibited significant superiority over other treatments in grain yield (5.52 t/ha), total dry matter (20.14 t/ha), and Harvest Index (25.21). These results suggest that growth inhibitors can effectively modulate the source-sink relationship, thereby enhancing grain yield.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"56 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141110618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-22DOI: 10.31830/2348-7542.2024.roc-1069
T. X. P. TRAN, D. H. Tran
One way to effectively use rice straw without causing environmental pollution is to produce biochar and reuse it to improve rice yield and soil properties. Using rice straw biochar has increased rice productivity and can reduce chemical fertilizers. Therefore, this study aimed to evaluate the effects of rice straw biochar application with reduced chemical fertilizer on the growth and yield of rice at Quang Phu commune, Quang Dien district, Thua Thien Hue province, Vietnam with four treatments of the co-application of rice straw biochar doses of 5, 7.5 and 10 t/ha and chemical fertilizers of 75, 50 and 25% of the recommended dose. Growth parameters, yield components, yield of rice, and soil property were investigated. The results showed that the rice straw biochar had a positive effect on the growth, yield of rice, and soil properties. The co-application of rice straw biochar of the level of 10 t/ha and chemical fertilizer of 25% of the recommended dose was optimal levels produced the highest growth and yield (63 t/ ha). The co-application of rice straw biochar and chemical fertilizers increased organic matter, improved both soil physical and chemical properties, and reduced chemical fertilizer use. As a result, the rice straw biochar may be recommended to farmers to apply for rice production.
{"title":"Impact of rice straw biochar and reduced chemical fertilizer on the growth and yield of rice (Oryza sativa L.) in Central Vietnam","authors":"T. X. P. TRAN, D. H. Tran","doi":"10.31830/2348-7542.2024.roc-1069","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1069","url":null,"abstract":"One way to effectively use rice straw without causing environmental pollution is to produce biochar and reuse it to improve rice yield and soil properties. Using rice straw biochar has increased rice productivity and can reduce chemical fertilizers. Therefore, this study aimed to evaluate the effects of rice straw biochar application with reduced chemical fertilizer on the growth and yield of rice at Quang Phu commune, Quang Dien district, Thua Thien Hue province, Vietnam with four treatments of the co-application of rice straw biochar doses of 5, 7.5 and 10 t/ha and chemical fertilizers of 75, 50 and 25% of the recommended dose. Growth parameters, yield components, yield of rice, and soil property were investigated. The results showed that the rice straw biochar had a positive effect on the growth, yield of rice, and soil properties. The co-application of rice straw biochar of the level of 10 t/ha and chemical fertilizer of 25% of the recommended dose was optimal levels produced the highest growth and yield (63 t/ ha). The co-application of rice straw biochar and chemical fertilizers increased organic matter, improved both soil physical and chemical properties, and reduced chemical fertilizer use. As a result, the rice straw biochar may be recommended to farmers to apply for rice production.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"3 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141108507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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