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Zbornik radova / XXVII savetovanje o biotehnologiji sa međunarodnim učešćem最新文献

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CHARACTERISTICS OF OCTADECYLAMINE-STABILIZED LIPOSOMES CONTAINING SOY PROTEIN HYDROLYSATES 含有大豆蛋白水解物的十八胺稳定脂质体的特性
Neda Pavlović, Jelena Mijalković, Verica B. Đorđević, B. Bugarski, Zorica D. Knežević Jugović
Liposomes have been researched as carriers for encapsulation of bioactive protein hydrolysate, despite low stability which makes them difficult to use. Using cholesterol or octadecylamine as lipid membrane stabilizers, soy protein hydrolysate is effectively encapsulated in liposomes created with phospholipon. Presence of both stabilizers concurrently resulted the highest efficiency 49.7%, while increasing the phospholipon mass had no effect. Particle size was raised (265 to 335 nm) as a result of the octadecylamine addition, and zeta potential was positive (36.7 mV), as compared to the cholesterol addition (-25.1 mV). Liposomes enabled extended hydrolysate release with retained its bioactivity.
脂质体作为生物活性蛋白水解产物的包封载体已被研究,尽管其稳定性较低,使其难以使用。使用胆固醇或十八乙胺作为脂质膜稳定剂,大豆蛋白水解物被有效地包裹在由磷脂产生的脂质体中。两种稳定剂同时存在时,效率最高(49.7%),而增加磷脂质量则没有效果。与胆固醇添加量(-25.1 mV)相比,十八胺添加量提高了颗粒尺寸(265 ~ 335 nm), zeta电位为正(36.7 mV)。脂质体可以延长水解产物的释放时间,同时保持其生物活性。
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引用次数: 0
UTICAJ ODGAJIVAČKIH USLOVA NA VARIJABILNOST MIKROKLIMATSKIH PARAMETARA U OBJEKTIMA ZA DRŽANJE MLEČNIH KRAVA U REPUBLICI SRBIJI
Nikolija Gligović, V. Bogdanović, R. Đedović, D. Stanojević, K. Zeljić
The aim of this study was to examine the breeding and housing conditions and their impact on the manifestation of variability of microclimatic parameters in facilities for keeping dairy cows. The systematic measurement of microclimate parameters was performed in buildings, that is temperature ( ) and relative humidity of air (RV%) in the period from 2014 to 2020 was performed on 12 farms from 6 administrative districts in the Republic of Serbia. A total of 478328 individual hourly measurements of temperature and relative humidity of air were analyzed. The fixed model, which included factors of farm size, month, hour, and farm for the manifestation of THI with a statistically high influence of all factors involved. All surveyed farms had a high potential for the development of heat stress in June, July and August. The greatest variability of THI was between small and large accommodation farms. Medium-capacity farms had consistently the highest average THI values and potential for heat stress development.
本研究的目的是考察奶牛饲养和饲养条件及其对奶牛饲养设施小气候参数变异性表现的影响。在塞尔维亚共和国6个行政区的12个农场进行了2014 - 2020年建筑小气候参数的系统测量,即温度()和空气相对湿度(RV%)。共分析了478328个单独的每小时空气温度和相对湿度测量值。固定模型包括农场规模、月份、小时和农场等因素,所有因素对THI的影响在统计上都很高。所有被调查猪场在6月、7月和8月发生热应激的可能性较大。THI在小型和大型住宿农场之间变化最大。中等容量农场始终具有最高的平均THI值和热应激发展潜力。
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引用次数: 0
PRIMENA OPTIMALNE METODE ZA OBRAČUN AMORTIZACIJE TRAKTORA I KOMBAJNA
Ranko Koprivica, Biljana Veljković, Marijana Gavrilović, Almir Muhović, Dragan Terzić, Dragoslav Đokić
The paper presents depreciation calculation of new MTZ 952 tractor and John Deere T 550 combine. Two time (linear and degressive) and functional methods for depreciation calculation were applied. After exploitation period of use, by calculating the annual depreciation of fixed assets, farmers can more easily plan the purchase of new machines. Also, the results of depreciation costs help farmers in making rational decisions on purchase of new machinery, or possible services engagement machines of other farmers. Taking into account costs of depreciation, prices of services provided by mechanization can be realistically determined, so based on that, farmers will choose what is more profitable.
本文介绍了新型MTZ 952拖拉机和约翰迪尔t550联合收割机的折旧计算方法。采用两种时间(线性和递减)和函数法进行折旧计算。经过开发使用期后,通过计算固定资产的年折旧,农民可以更容易地计划购买新机器。此外,折旧成本的结果有助于农民在购买新机器或可能服务其他农民的机器时做出理性决策。考虑到折旧成本,机械化提供的服务价格可以实际确定,因此农民将在此基础上选择更有利可图的服务。
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引用次数: 0
SADRŽAJ VITAMINA C U EKSTRAKTIMA ŠIPURKA
Danijela Stojković, Verica Jevtić, Maja Đukić, Đorđe Petrović, Sandra Jovičić Milić, Marijana Kasalović
Rosehip is rich in vitamin C. It also contains provitamin A, B vitamins, vitamin K, as well as many minerals. Rosehip is most often used to produce jams and marmalades. It can also be used to produce drinks because its specific taste and aroma significantly contributes to the appearance and taste of juices. Tea is also the most famous way to use rosehips. Due to the high content of vitamin C and pectin, it can be used for canning food in cooking and for preparing winter food. Medicinal wine can also be made from rose hips. In this paper, extracts of the plant Rosehip obtained by different extraction methods, were examined. The content of extracted substances was determined, as well as the content of vitamin C in order to determine which method will give the best yields of extracts.
玫瑰果富含维生素c,还含有维生素原A、B族维生素、维生素K以及许多矿物质。玫瑰果最常用于制作果酱和果酱。它也可以用来生产饮料,因为它的特殊味道和香气对果汁的外观和味道有很大的影响。茶也是最著名的使用玫瑰果的方法。由于维生素C和果胶含量高,可用于烹调和制作冬季食品的罐头。药酒也可以用玫瑰果酿造。本文研究了不同提取方法对玫瑰果提取物的影响。测定了提取物质的含量和维生素C的含量,以确定哪种提取方法的提取率最高。
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引用次数: 0
ANOTHER LOOK AT THE BIOLOGICAL ROLES OF A PLANT ALKALOID-BERBERINE 另一种植物生物碱——小檗碱的生物学作用
Marko R Antonijević, Žiko B. Milanović, Edina H. Avdović, Dušica M Simijonović, Z. Marković
For millennia, berberine extracts or berberine itself has been the effective traditional drug with wide application due to its broad spectrum of antibiotic activity. A significant aspect of the berberine’s physiological activity that is often overlooked is the ability to go through the blood-brain barrier and has an impact on different processes and irregularities in the brain such as dementia and Alzheimer’s disease. Potential inhibitory activity towards enzymes for which is believed to be involved in these diseases, in this paper is confirmed by molecular docking simulations. Binding energies suggest that berberine exhibits good potential inhibitory activity and confirms that one of the aspects of suppression of Alzheimer’s disease and dementia is the inhibition of cholinesterase enzymes.
几千年来,小檗碱提取物或小檗碱本身由于具有广谱的抗生素活性,一直是有效的传统药物,具有广泛的应用。小檗碱生理活动的一个重要方面经常被忽视,即通过血脑屏障的能力,并对大脑中的不同过程和不规则性产生影响,如痴呆和阿尔茨海默病。本文通过分子对接模拟证实了对被认为与这些疾病有关的酶的潜在抑制活性。结合能提示小檗碱具有良好的潜在抑制活性,证实了抑制阿尔茨海默病和痴呆的一个方面是抑制胆碱酯酶。
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引用次数: 0
UTICAJ INOKULACIJE SEMENA NS NITRAGINOM NA PRINOS SOJE
Gorica Cvijanović, Vojin Đukić, Marija Bajagić, Vesna Stepić, Vojin Cvijanović, Gordana Dozet
Soybeans are legumes capable of absorbing elemental nitrogen from the air thanks to symbiosis with symbiotic bacteria. Inoculation of soybean seeds with highly effective bacteria increases nitrogen uptake and has a positive effect on the achieved yield. The aim of this paper is to consider the effect of NS Nitragine application on the yield of six soybean cultivars of different maturation groups. Inoculation statistically significantly increased soybean yield, and the increase ranged from 6.13% in the cultivar NS Zoja to 8.49% in the cultivar Sava.
大豆是一种豆科植物,由于与共生细菌的共生关系,它能够从空气中吸收元素氮。用高效菌接种大豆种子可提高氮素吸收量,对大豆产量有积极影响。研究了施用硝酸根碱对6个不同成熟期大豆品种产量的影响。接种大豆可显著提高大豆产量,增产幅度从NS Zoja的6.13%到Sava的8.49%不等。
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引用次数: 0
PROCENA OKSIDATIVNOG STRESA KUKURUZA I SUNCOKRETA TRETIRANIH HOLNOM KISELINOM U RAZLIČITIM MEDIJUMIMA – SKRINING TEST
Marina Crnković, Jovana Šućur, Đorđe Malenčić, Suzana Jovanović-Šanta
The effect of cholic acid (CA) dissolved in two different media, namely Hogland nutrient solution and distilled water, on the oxidative stress of maize (Zea mays, L.) and sunflower (Helianthus annuus, L.) was assessed by measuring the intensity of lipid peroxidation. The aim of this screening is to observe possible differences in the results obtained from plants treated with an aqueous solution of cholic acid and those treated with a solution of cholic acid in Hogland's solution. Oxidative stress was assessed on the basis of the estimated intensity of lipid peroxidation (LP), and on the basis of the measured amount of the resulting LP final product, malondialdehyde (MDA). The results showed faster production of MDA in plants treated with cholic acid in Hogland's solution, which indicates that nutrients affect the action of CA.
通过测定玉米(Zea mays, L.)和向日葵(Helianthus annuus, L.)的脂质过氧化强度,研究了胆酸(CA)溶解于Hogland营养液和蒸馏水两种不同培养基中对玉米(Zea mays, L.)和向日葵(Helianthus annuus, L.)氧化应激的影响。筛选的目的是观察用胆酸水溶液处理的植物和用Hogland溶液中胆酸溶液处理的植物所获得的结果可能存在的差异。根据脂质过氧化(LP)的估计强度和测量得到的LP最终产物丙二醛(MDA)的量来评估氧化应激。结果表明,在Hogland’s溶液中使用胆酸处理的植株产生MDA的速度更快,说明营养物质影响了CA的作用。
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引用次数: 0
INFLUENCE OF INDIGENOUS AND COMMERCIAL YEASTS ON THE PRODUCTION OF RED WINE FROM VRANEC, MERLOT AND FRANKOVKA IN VINICA WINE REGION 本地酵母和商业酵母对维尼察葡萄酒产区弗拉内克、梅洛和弗兰科夫卡红葡萄酒生产的影响
Antonio Petrov, Fidanka Ilieva, Sanja Velichkovich Kostadinovska, V. Dimovska
The aim of this study was the influence of yeasts on the production of red wines from the wine region of Vinica. The research was conducted on three grape varieties, cultivated in 3 different micro locations, each of which has its own unique characteristics and different altitudes. The three grape varieties were included: Vranec (cultivated in the area of Krshla, Vinica, at an altitude of 400m), Frankovka (cultivated in the area of Baltaci, Vinica, at an altitude of 520m) and Merlot (cultivated in Dragobrashte, Vinica, at an altitude of 540m ). The main goal of the research is to define the effect of the yeast on the quality of young wines. The indigenous types of yeast already present in the grapes (wild yeasts) and the commercial types from the Saccharomyces cerevisiae family which were isolated for use in international production of red wines of average tannin value (SELECTYS® LA DÉLICIEUSE) were applied. The research of the wines was carried out in the Wine Institute at the State Phytosanitary Laboratory in Skopje(North Macedonia) and involved examination of the wine quality parameters such micro and macro wine elements with ICP/MS and FOSS WINESCAN, parameters for alcohol, sugar, total acids, volatile acids, pH, total/volatile acids. This research gave insight of the correlation between samples of the wine and their parameters. It was further expanded with parameters for micro and macro wine elements which also shows the correlation of the wine samples. The wine region of Vinica is a developing region of great potential and this is the first step to presenting a vision for wine development in the region.
本研究的目的是酵母对葡萄产区红葡萄酒生产的影响。研究对象是三个葡萄品种,分别在3个不同的微位置种植,每个微位置都有自己独特的特征和不同的海拔高度。这三个葡萄品种包括:Vranec(种植在Vinica Krshla地区,海拔400米),Frankovka(种植在Vinica Baltaci地区,海拔520米)和Merlot(种植在Vinica Dragobrashte地区,海拔540米)。研究的主要目的是确定酵母对年轻葡萄酒质量的影响。已经存在于葡萄中的本地酵母类型(野生酵母)和酿酒酵母家族的商业类型,这些类型被分离出来用于国际生产平均单宁值的红葡萄酒(SELECTYS®LA DÉLICIEUSE)。葡萄酒的研究是在斯科普里(北马其顿)国家植物检疫实验室的葡萄酒研究所进行的,包括用ICP/MS和FOSS WINESCAN检查葡萄酒的质量参数,如微观和宏观葡萄酒元素,酒精,糖,总酸,挥发酸,pH值,总/挥发酸的参数。这项研究使人们了解了葡萄酒样品与其参数之间的相关性。进一步扩展了微观和宏观葡萄酒元素参数,也显示了葡萄酒样品的相关性。维尼察葡萄酒产区是一个具有巨大潜力的发展中地区,这是展示该地区葡萄酒发展愿景的第一步。
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引用次数: 1
UTICAJ APLIKACIJE NIACINA NA VREDNOST NJEGOVIH VITAMERA U KRVI KRAVA U RANOJ LAKTACIJI 实用的烟酸和维生素以及对人体健康的益处
Talija Hristovska, Kosta Petrović, Marko Cincović, Branislava Belić, Maja Došenović Marinković, Radojica Đoković, Miloš Petrović, Dražen Kovačević
Vitamin niacin is of great importance for energy metabolism. Physiological niacin is incorporated into the coenzyme nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). The aim of this study was to determine the concentration of NAD and NADP in the blood of cows during the application of niacin in the peripartum period. The value of these vitamins depends on the peripartum week, regardless of the constant exogenous source of niacin.
维生素烟酸对能量代谢非常重要。生理性烟酸被纳入辅酶烟酰胺腺嘌呤二核苷酸(NAD)和烟酰胺腺嘌呤二核苷酸磷酸(NADP)。本研究旨在测定围产期应用烟酸对奶牛血液中NAD和NADP浓度的影响。这些维生素的价值取决于围产周,而不考虑烟酸的恒定外源。
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引用次数: 0
ESSENTIAL OILS AS POTENTIAL BIOCONTROL PRODUCTS AGAINST PLANT PATHOGENS AND WEEDS: IN VITRO CULTURE APPROACH 精油作为潜在的植物病原体和杂草生物防治产品:离体培养方法
B. Uzelac, D. Stojičić, S. Budimir, Svetlana Tošić, B. Zlatković, Saša Blagojević, Branislav Manić, Mirjana Janjanin, V. Slavkovska
Secondary metabolism in plant plays a major role in the survival of the plant in its ecosystem, mediating the interaction of the plant with its environment. Plant bioactive compounds are biosynthesized as a defensive strategy of plants in response to natural perturbations. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these prospective biocontrol products is in vitro plant tissue culture. Our data suggest that the metabolic potential of in vitro shoot cultures of selected species can be manipulated by varying in vitro culture conditions.
植物次生代谢是植物与环境相互作用的中介,对植物在生态系统中的生存起着重要作用。植物生物活性化合物是植物应对自然干扰的一种防御策略。从所研究的小麦冬属(Micromeria)和羊角草属(Clinopodium)精油中检测到的主要单萜类化合物具有多种生物学效应。生产这些有前景的生物防治产品的一种替代方法是离体植物组织培养。我们的数据表明,选定物种的体外芽培养的代谢势可以通过改变体外培养条件来操纵。
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引用次数: 0
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Zbornik radova / XXVII savetovanje o biotehnologiji sa međunarodnim učešćem
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