Pub Date : 2024-03-31DOI: 10.17576/jsm-2024-5303-13
F. R. Dewi, Ulie Vianissa Dwiutami Tambunan, Putri Amatul Bari, Muhammad Ardiansyah Farid, Nadira Aisha Anjani, Sri Puji Astuti Wahyuningsih, Amy Yi Hsan Saik, Y. Yong, Vuanghao Lim, Wen Nee Tan, Mohammed Abdullah Mahdi Alshawsh
Curcumin and tetrahydrocurcumin (THC) are known for their anticancer properties, but limited solubility in water hinders their effectiveness against cancer. In this study, we conducted an in silico exploration of β-cyclodextrin's potential to form inclusion complexes with curcumin or THC. The aim of this study was to assess the potential of curcumin and THC inclusion complexes to inhibit vascular endothelial growth factor (VEGF) signaling pathway, a key element in carcinogenesis. The in silico analysis involved multiple stages, such as bioactive compound preparation, biological activity prediction, 3D structure retrieval of VEGF and VEGFR, protein-ligand docking, and visualization. The results of the study demonstrated that both the curcumin- and THC-inclusion complexes exhibit a lower requirement for binding free energy to interact with VEGFR compared to curcumin or THC molecules alone. When VEGFR binds with curcumin, the curcumin-inclusion complex, or the THC-inclusion complex before interacting with VEGF, there is a notable increase in the binding free energy for the VEGF-VEGFR interaction. Specifically, the presence of THC-inclusion complex demonstrates the highest binding free energy for the VEGF-VEGFR interaction. The molecular dynamic simulation study shows that when VEGFR binds with curcumin, curcumin-inclusion complex, or THC-inclusion complex, the fluctuation of amino acid residues in VEGFR decreases compared to the VEGFR protein structure before binding with these molecules. In conclusion, this study suggests that the formation of inclusion complexes holds considerable promise for enhancing the anticancer potential of curcumin and THC by augmenting their anti-angiogenic activity.
{"title":"Formation of Inclusion Complex of Curcumin and Tetrahydrocurcumin Prevents Angiogenesis by Inhibiting VEGF Activity: An in-Silico Study","authors":"F. R. Dewi, Ulie Vianissa Dwiutami Tambunan, Putri Amatul Bari, Muhammad Ardiansyah Farid, Nadira Aisha Anjani, Sri Puji Astuti Wahyuningsih, Amy Yi Hsan Saik, Y. Yong, Vuanghao Lim, Wen Nee Tan, Mohammed Abdullah Mahdi Alshawsh","doi":"10.17576/jsm-2024-5303-13","DOIUrl":"https://doi.org/10.17576/jsm-2024-5303-13","url":null,"abstract":"Curcumin and tetrahydrocurcumin (THC) are known for their anticancer properties, but limited solubility in water hinders their effectiveness against cancer. In this study, we conducted an in silico exploration of β-cyclodextrin's potential to form inclusion complexes with curcumin or THC. The aim of this study was to assess the potential of curcumin and THC inclusion complexes to inhibit vascular endothelial growth factor (VEGF) signaling pathway, a key element in carcinogenesis. The in silico analysis involved multiple stages, such as bioactive compound preparation, biological activity prediction, 3D structure retrieval of VEGF and VEGFR, protein-ligand docking, and visualization. The results of the study demonstrated that both the curcumin- and THC-inclusion complexes exhibit a lower requirement for binding free energy to interact with VEGFR compared to curcumin or THC molecules alone. When VEGFR binds with curcumin, the curcumin-inclusion complex, or the THC-inclusion complex before interacting with VEGF, there is a notable increase in the binding free energy for the VEGF-VEGFR interaction. Specifically, the presence of THC-inclusion complex demonstrates the highest binding free energy for the VEGF-VEGFR interaction. The molecular dynamic simulation study shows that when VEGFR binds with curcumin, curcumin-inclusion complex, or THC-inclusion complex, the fluctuation of amino acid residues in VEGFR decreases compared to the VEGFR protein structure before binding with these molecules. In conclusion, this study suggests that the formation of inclusion complexes holds considerable promise for enhancing the anticancer potential of curcumin and THC by augmenting their anti-angiogenic activity.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140359767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.17576/jsm-2024-5303-17
Nur Farah Bazilah Wakhi Anuar, M. Z. Omar, M. S. Salleh, Wan Fathul Hakim W. Zamri, Afifah Md Ali
Aluminium metal matrix composites are increasingly being used in numerous industries due to their lightweight nature and high strength. The incorporation of graphene into aluminium metal matrix composites has garnered significant interest owing to graphene's capacity to enhance numerous properties concurrently. Hence, this study aimed to fabricate a 0.3 wt.% graphene nanoplatelets reinforced A356 alloy (GNP-A356) composite through stir casting followed by thixoforming and short T6 heat treatment processes. It also evaluated the microstructure and mechanical properties of GNP-A356 after the thixoforming process and short T6 heat treatment. The microstructure of alloy and composite was confirmed by optical microstructure, field emission scanning electron microscopy images, and X-ray diffraction. Microstructure investigations demonstrate the impact of the stirring process on the structural transformation of the 𝛼-Al phase from the dendritic into a rosette-like and globular-like structure. The result also indicated that there was a transformation of eutectic silicon from the shape of a needle-like to spheroid structure after a short heat treatment, showing the efficiency of T6 heat treatment with a shorter time of solution treatment and ageing. Moreover, the increase in relative density and addition of reinforcement led to a significant increment in hardness. The result shows the hardness improved by 35.77% and 77.8% for as-cast and heat-treated composites, respectively, compared to A356 alloy.
{"title":"Effect of Short Heat Treatments on the Microstructural Evolution and Hardness of Thixoformed Graphene Reinforced Aluminium Composites","authors":"Nur Farah Bazilah Wakhi Anuar, M. Z. Omar, M. S. Salleh, Wan Fathul Hakim W. Zamri, Afifah Md Ali","doi":"10.17576/jsm-2024-5303-17","DOIUrl":"https://doi.org/10.17576/jsm-2024-5303-17","url":null,"abstract":"Aluminium metal matrix composites are increasingly being used in numerous industries due to their lightweight nature and high strength. The incorporation of graphene into aluminium metal matrix composites has garnered significant interest owing to graphene's capacity to enhance numerous properties concurrently. Hence, this study aimed to fabricate a 0.3 wt.% graphene nanoplatelets reinforced A356 alloy (GNP-A356) composite through stir casting followed by thixoforming and short T6 heat treatment processes. It also evaluated the microstructure and mechanical properties of GNP-A356 after the thixoforming process and short T6 heat treatment. The microstructure of alloy and composite was confirmed by optical microstructure, field emission scanning electron microscopy images, and X-ray diffraction. Microstructure investigations demonstrate the impact of the stirring process on the structural transformation of the 𝛼-Al phase from the dendritic into a rosette-like and globular-like structure. The result also indicated that there was a transformation of eutectic silicon from the shape of a needle-like to spheroid structure after a short heat treatment, showing the efficiency of T6 heat treatment with a shorter time of solution treatment and ageing. Moreover, the increase in relative density and addition of reinforcement led to a significant increment in hardness. The result shows the hardness improved by 35.77% and 77.8% for as-cast and heat-treated composites, respectively, compared to A356 alloy.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140358365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.17576/jsm-2024-5303-07
Nur Lisa Mohd Yusoff, Durriyyah Sharifah Hasan Adli, S. Tiong
Toluene exposure has been associated with detrimental effects on the central nervous system. Discovering natural products that can offer neuroprotection following toluene exposure is an essential alternative. Nigella sativa (NS), a popular natural supplement, is a good candidate due to its antioxidant and neuroprotective properties. The study aimed to investigate the protective potentials of NS against toluene, on recognition memory performance in the Novel Object Recognition (NOR) test and cellular morphometric measurements of hippocampal CA1 pyramidal neurons. Adult male ICR mice (n=30) were randomly divided into five groups: Group 1 (G1) - corn oil (CO), G2 – Toluene (TOL), G3 - Toluene and NS seed suspension (TOL-NSS), G4 - Toluene and NS oil (TOL-NSO), G5 - Toluene and thymoquinone (TOL-TQ). NS supplementations were administered orally once daily for 14 consecutive days while 500 mg/kg b.w. toluene was administered intraperitoneally from day 8 until day 14. Behavioural NOR test was conducted. Subsequently, mice were intracardially perfused and harvested brains were histologically processed. The somatic size and shape of hippocampal CA1 pyramidal neurons were quantified according to specific morphometric parameters. Toluene reduced recognition memory performance of mice and somatic size of hippocampal CA1 pyramidal neurons. Contradictorily, TQ, NSO, and NSS improved mice recognition memory and somatic size of hippocampal CA1 pyramidal neurons. Somatic shape of hippocampal CA1 pyramidal was unaffected by the various treatments. Although non-significant differences were observed, the results indicate the tendency for toluene to cause impairment, while NS supplementations to improve mice recognition memory performance and brain hippocampal neuronal structure.
{"title":"Nigella Sativa Supplementations Attenuate Recognition Memory and Cellular Morphometric Impairments Induced by Toluene Administration in Mice","authors":"Nur Lisa Mohd Yusoff, Durriyyah Sharifah Hasan Adli, S. Tiong","doi":"10.17576/jsm-2024-5303-07","DOIUrl":"https://doi.org/10.17576/jsm-2024-5303-07","url":null,"abstract":"Toluene exposure has been associated with detrimental effects on the central nervous system. Discovering natural products that can offer neuroprotection following toluene exposure is an essential alternative. Nigella sativa (NS), a popular natural supplement, is a good candidate due to its antioxidant and neuroprotective properties. The study aimed to investigate the protective potentials of NS against toluene, on recognition memory performance in the Novel Object Recognition (NOR) test and cellular morphometric measurements of hippocampal CA1 pyramidal neurons. Adult male ICR mice (n=30) were randomly divided into five groups: Group 1 (G1) - corn oil (CO), G2 – Toluene (TOL), G3 - Toluene and NS seed suspension (TOL-NSS), G4 - Toluene and NS oil (TOL-NSO), G5 - Toluene and thymoquinone (TOL-TQ). NS supplementations were administered orally once daily for 14 consecutive days while 500 mg/kg b.w. toluene was administered intraperitoneally from day 8 until day 14. Behavioural NOR test was conducted. Subsequently, mice were intracardially perfused and harvested brains were histologically processed. The somatic size and shape of hippocampal CA1 pyramidal neurons were quantified according to specific morphometric parameters. Toluene reduced recognition memory performance of mice and somatic size of hippocampal CA1 pyramidal neurons. Contradictorily, TQ, NSO, and NSS improved mice recognition memory and somatic size of hippocampal CA1 pyramidal neurons. Somatic shape of hippocampal CA1 pyramidal was unaffected by the various treatments. Although non-significant differences were observed, the results indicate the tendency for toluene to cause impairment, while NS supplementations to improve mice recognition memory performance and brain hippocampal neuronal structure.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140358579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.17576/jsm-2024-5303-14
Faridah Ismail, Yusmazura Zakaria, M. L. MD ISA, Nik Fakhuruddin Nik Hassan, Suat Cheng Tan
Cancer stem cells (CSCs) are a small population of tumour cells that are responsible for tumour initiation, metastases, recurrence, and resistance to conventional therapy. Hence, targeting CSCs is crucial in the fight against cancer. SF1, a standardised fraction from Clinacanthus nutans leaf extract, has been reported to exhibit potent and selective antineoplastic effects against cervical cancer cells. In this study, the potential of SF1 to inhibit the stemness of cervical cancer stem-like cells has been evaluated. SF1 extraction was carried out using the dry column vacuum chromatography technique. SiHa cell lines were cultured as spheres in CSC-conditioned medium (cervospheres), and the IC50 of SF1 against cervospheres was determined using the OZBlue Cell Viability Kit. The effects of SF1 on the cervosphere’s stemness markers, including CD49f, CK17, SOX2, OCT4, and NANOG, were assessed using a flow cytometry assay. Self-renewal inhibition and anti-tumorigenesis effects of SF1 in cervospheres were evaluated using a sphere formation assay and a xenograft mouse model. The present study shows that SF1 treatment at an IC50 of 17.07 µg/mL inhibited the proliferation, self-renewal, and tumorigenic capacity of SiHa cervospheres in vitro and in vivo. A decrease in the expressions of CK17, SOX2, CD49f, and OCT4 in cervical CSCs indicated that SF1’s inhibitory effects were also associated with the suppression of stemness markers. These results suggest that SF1 possesses an antitumor effect against cervical CSCs and may be regarded as a promising approach to the development of targeted anticancer agents for cervical cancer.
{"title":"SF1: A Standardised Fraction of Clinacanthus nutans That Inhibits the Stemness Properties of Cancer Stem-Like Cells Derived from Cervical Cancer","authors":"Faridah Ismail, Yusmazura Zakaria, M. L. MD ISA, Nik Fakhuruddin Nik Hassan, Suat Cheng Tan","doi":"10.17576/jsm-2024-5303-14","DOIUrl":"https://doi.org/10.17576/jsm-2024-5303-14","url":null,"abstract":"Cancer stem cells (CSCs) are a small population of tumour cells that are responsible for tumour initiation, metastases, recurrence, and resistance to conventional therapy. Hence, targeting CSCs is crucial in the fight against cancer. SF1, a standardised fraction from Clinacanthus nutans leaf extract, has been reported to exhibit potent and selective antineoplastic effects against cervical cancer cells. In this study, the potential of SF1 to inhibit the stemness of cervical cancer stem-like cells has been evaluated. SF1 extraction was carried out using the dry column vacuum chromatography technique. SiHa cell lines were cultured as spheres in CSC-conditioned medium (cervospheres), and the IC50 of SF1 against cervospheres was determined using the OZBlue Cell Viability Kit. The effects of SF1 on the cervosphere’s stemness markers, including CD49f, CK17, SOX2, OCT4, and NANOG, were assessed using a flow cytometry assay. Self-renewal inhibition and anti-tumorigenesis effects of SF1 in cervospheres were evaluated using a sphere formation assay and a xenograft mouse model. The present study shows that SF1 treatment at an IC50 of 17.07 µg/mL inhibited the proliferation, self-renewal, and tumorigenic capacity of SiHa cervospheres in vitro and in vivo. A decrease in the expressions of CK17, SOX2, CD49f, and OCT4 in cervical CSCs indicated that SF1’s inhibitory effects were also associated with the suppression of stemness markers. These results suggest that SF1 possesses an antitumor effect against cervical CSCs and may be regarded as a promising approach to the development of targeted anticancer agents for cervical cancer.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140359888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.17576/jsm-2024-5303-06
Izyan Mohd Idris, F. Nordin, Nur Jannaim Muhamad, Siti Zuleha Idris, G. Tye, Wan Safwani Wan Kamarul Zaman, Min Hwei Ng
Lentiviruses are a highly robust gene delivery system capable of in vitro and in vivo gene transfer into multiple cell types. Recent fourth-generation lentiviral systems have been designed for enhanced safety, however, the increased recombination events required to produce infective lentiviral particles may reduce production efficiency. A set protocol for all types of target cells is not recommended and optimization of conditions for gene transfer into different target cells is required. In this study, we aim to evaluate the efficiency and reproducibility of lentiviral production using a fourth-generation lentiviral packaging system and identify optimal parameters for successful transduction in two different cell models, adherent and suspension cells. Lentiviral production, effect of viral volume, sustained gene expression and transduction adjuvants on adherent and suspension gene- cell models were evaluated. Transfection and transduction efficiency of lentiviruses was evaluated by fluorescence microscopy and flow cytometry. This study demonstrates that production of green fluorescent protein (GFP)- lentiviruses using the fourth-generation lentiviral packaging is consistent and reproducible. Optimal transduction of adherent cell types is achieved at lower multiplicity of infection (MOI) compared to suspension cells and produces GFP-expressing cells with higher intensity. Expression of GFP is sustained in all cell types over multiple passages. Polycation DEAE-dextran was determined to improve transduction in suspension cells, however, provides similar transduction efficiency as polybrene in adherent cells. In conclusion, fourth generation lentiviral system reproducibly generates high titre lentiviruses capable of infecting multiple cell types, however transduction protocols for different cell types require further optimization.
{"title":"Evaluation of Lentiviral Based Gene Delivery System in Adherent and Suspension in vitro Cell Models","authors":"Izyan Mohd Idris, F. Nordin, Nur Jannaim Muhamad, Siti Zuleha Idris, G. Tye, Wan Safwani Wan Kamarul Zaman, Min Hwei Ng","doi":"10.17576/jsm-2024-5303-06","DOIUrl":"https://doi.org/10.17576/jsm-2024-5303-06","url":null,"abstract":"Lentiviruses are a highly robust gene delivery system capable of in vitro and in vivo gene transfer into multiple cell types. Recent fourth-generation lentiviral systems have been designed for enhanced safety, however, the increased recombination events required to produce infective lentiviral particles may reduce production efficiency. A set protocol for all types of target cells is not recommended and optimization of conditions for gene transfer into different target cells is required. In this study, we aim to evaluate the efficiency and reproducibility of lentiviral production using a fourth-generation lentiviral packaging system and identify optimal parameters for successful transduction in two different cell models, adherent and suspension cells. Lentiviral production, effect of viral volume, sustained gene expression and transduction adjuvants on adherent and suspension gene- cell models were evaluated. Transfection and transduction efficiency of lentiviruses was evaluated by fluorescence microscopy and flow cytometry. This study demonstrates that production of green fluorescent protein (GFP)- lentiviruses using the fourth-generation lentiviral packaging is consistent and reproducible. Optimal transduction of adherent cell types is achieved at lower multiplicity of infection (MOI) compared to suspension cells and produces GFP-expressing cells with higher intensity. Expression of GFP is sustained in all cell types over multiple passages. Polycation DEAE-dextran was determined to improve transduction in suspension cells, however, provides similar transduction efficiency as polybrene in adherent cells. In conclusion, fourth generation lentiviral system reproducibly generates high titre lentiviruses capable of infecting multiple cell types, however transduction protocols for different cell types require further optimization.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140360512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.17576/jsm-2024-5303-18
Norhayati Rosli, Muhammad Fahmi Bin Ahmad Zuber, Ali Turab
With the advent of the internet, social media of Facebook and Twitter, as well as the communication technology of WhatsApp and Telegram, the speed and scope of the rumour dissemination has been expanded. Understanding the characterization of rumour dissemination and how it spreads can help in mitigation measures to avoid the spread of the rumour. Therefore, it is crucial to propose a mathematical model, and in particular this paper is concerned with the epidemic model to understand the dissemination of the rumour in social network. The mechanism of rumour propagation is behaving like infectious diseases spread; hence this research adopted the epidemiological model approach. In this network, the compartment is divided into susceptible, ignorant, propagation and stiflers. The basic influence number, the equilibrium points of rumour-free and the endemic equilibrium state were obtained and discussed. For the local stability, the Next Generation Matrix was used. Numerical simulation is performed to understand the dynamics of the spread of rumour in a population or social networks, its impact in a population, and adjusting mechanisms in curbing the spread of rumour.
{"title":"Mathematical Modelling of a Rumour Spreading with the Attitude of Adjusting Mechanisms","authors":"Norhayati Rosli, Muhammad Fahmi Bin Ahmad Zuber, Ali Turab","doi":"10.17576/jsm-2024-5303-18","DOIUrl":"https://doi.org/10.17576/jsm-2024-5303-18","url":null,"abstract":"With the advent of the internet, social media of Facebook and Twitter, as well as the communication technology of WhatsApp and Telegram, the speed and scope of the rumour dissemination has been expanded. Understanding the characterization of rumour dissemination and how it spreads can help in mitigation measures to avoid the spread of the rumour. Therefore, it is crucial to propose a mathematical model, and in particular this paper is concerned with the epidemic model to understand the dissemination of the rumour in social network. The mechanism of rumour propagation is behaving like infectious diseases spread; hence this research adopted the epidemiological model approach. In this network, the compartment is divided into susceptible, ignorant, propagation and stiflers. The basic influence number, the equilibrium points of rumour-free and the endemic equilibrium state were obtained and discussed. For the local stability, the Next Generation Matrix was used. Numerical simulation is performed to understand the dynamics of the spread of rumour in a population or social networks, its impact in a population, and adjusting mechanisms in curbing the spread of rumour.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140360691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.17576/jsm-2024-5303-08
Jing Wang, Tiejin Tong, Qiangjun Wu
This study was conducted to evaluate the effect of Paederia scandens on high-fat dietinduced non-alcoholic fatty liver disease (NAFLD) in a rat model and further show the therapeutic mechanisms of Paederia scandens. Thirty rats weighing 180 ± 12 g (6 weeks old) were randomly divided into three groups: A control group (CG), a high-fat diet model group (HF), and a Paederia scandens intervention group (PS). After 45 days, the rats' serum lipid metabolism, liver injury parameters, and liver proteomics were detected. The results indicated that dietary Paederia scandens significantly reduced the levels of triglycerides, total cholesterol, glucose, and low-density lipoprotein cholesterol in the NAFLD rats compared with those in the HF group. Meanwhile, decreased levels of alanine aminotransferase and aspartate transaminase were observed in rats of the PS group. In addition, 382 differentially abundant proteins were identified between the HF and PS groups. Protein-protein interaction network analysis identified 14 keystone proteins that might play critical roles in ameliorating NAFLD. In particular, Paederia scandens treatment significantly upregulated the levels of Hadh, Hadhb, Acadl, Acox1, Acox3, Cyp3a2, and Cyp1a1, which are involved in fatty acid β‑oxidation, PPAR, and cytochrome P450 signaling pathways. Hence, the data demonstrated that Paederia scandens ameliorates hepatic lipid accumulation and impairment by enhancing fatty acid β-oxidation and activating PPAR and cytochrome P450 signaling pathways. These data provide new insights into the treatment of NAFLD and suggest the potential of Paederia scandens as an effective therapy.
{"title":"Proteomics Reveals the Role of Paederia scandens in ameliorating Non-alcoholic Fatty Liver Disease in a Rat Model","authors":"Jing Wang, Tiejin Tong, Qiangjun Wu","doi":"10.17576/jsm-2024-5303-08","DOIUrl":"https://doi.org/10.17576/jsm-2024-5303-08","url":null,"abstract":"This study was conducted to evaluate the effect of Paederia scandens on high-fat dietinduced non-alcoholic fatty liver disease (NAFLD) in a rat model and further show the therapeutic mechanisms of Paederia scandens. Thirty rats weighing 180 ± 12 g (6 weeks old) were randomly divided into three groups: A control group (CG), a high-fat diet model group (HF), and a Paederia scandens intervention group (PS). After 45 days, the rats' serum lipid metabolism, liver injury parameters, and liver proteomics were detected. The results indicated that dietary Paederia scandens significantly reduced the levels of triglycerides, total cholesterol, glucose, and low-density lipoprotein cholesterol in the NAFLD rats compared with those in the HF group. Meanwhile, decreased levels of alanine aminotransferase and aspartate transaminase were observed in rats of the PS group. In addition, 382 differentially abundant proteins were identified between the HF and PS groups. Protein-protein interaction network analysis identified 14 keystone proteins that might play critical roles in ameliorating NAFLD. In particular, Paederia scandens treatment significantly upregulated the levels of Hadh, Hadhb, Acadl, Acox1, Acox3, Cyp3a2, and Cyp1a1, which are involved in fatty acid β‑oxidation, PPAR, and cytochrome P450 signaling pathways. Hence, the data demonstrated that Paederia scandens ameliorates hepatic lipid accumulation and impairment by enhancing fatty acid β-oxidation and activating PPAR and cytochrome P450 signaling pathways. These data provide new insights into the treatment of NAFLD and suggest the potential of Paederia scandens as an effective therapy.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140358449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.17576/jsm-2024-5303-02
Mohd Shahidi Alias, A. S. Mohd Rafie, Mohd Faisal Abdul Hamid, E. Gires, Khairul Dahri Mohd Aris
Understanding the atmospheric properties and patterns is crucial in empowering Malaysia's national aerospace blueprint, national space, and legislation. While various policies have been enacted and implemented, there is a lack of information on the wind profile, specifically the upper air across Peninsular Malaysia. Realising the need to establish a standard guideline for national reference, future research, space-aerospace application, and legislation, this study was performed to develop the first wind profile analysis of upper air in Peninsular Malaysia. Relevant data from the Malaysian Meteorology Department was collected for analysis and evaluation. Specifically, a meteorology balloon attached with a sounding radiosonde was used to record data at 0000UTC (0800 h LT) and 1200UTC (2000 h LT) on the 15th day of each month for 7 years (from 2015 to 2021) at the KLIA and Kuantan Meteorology Stations for the wind profiling analysis. The daily overall data collection was recorded accurately once the balloon's rising rate stabilises from vertical air current (katabatic or anabatic winds). Subsequently, the collected data were evaluated in terms of the minimum, maximum, and average wind speeds for each year and time. Finally, the average wind speed of each year and time were combined to generate the Peninsular Malaysia wind profile. Based on the results, the projected wind profile for both stations identified three peaks of discrete sine wave flow type with low-speed wind profile in Peninsular Malaysia. The three peaks amplified the highest air velocity, whereby the 1st and 2nd peaks were located at the troposphere layer from 9,000 m to 12,000 m altitude (average wind speed of 10.8 ms-1) and 12,000 m to 18,000 m altitude (average wind speed of 13.7 ms-1). The 3rd peak was located at the stratosphere layer from 18,000 m to 32,000 m altitude (average wind speed of 15.2 ms-1). Since East Malaysia is located on the same equatorial line, the wind profile is hypothetically the same and exhibits only slight differences. In short, the established wind profile of upper air in Peninsular Malaysia in this study would facilitate other future studies and assist long-term planning of Malaysia's airspace legislation.
{"title":"Wind Profiles in Peninsular Malaysia: A Comprehensive Upper Air Analysis","authors":"Mohd Shahidi Alias, A. S. Mohd Rafie, Mohd Faisal Abdul Hamid, E. Gires, Khairul Dahri Mohd Aris","doi":"10.17576/jsm-2024-5303-02","DOIUrl":"https://doi.org/10.17576/jsm-2024-5303-02","url":null,"abstract":"Understanding the atmospheric properties and patterns is crucial in empowering Malaysia's national aerospace blueprint, national space, and legislation. While various policies have been enacted and implemented, there is a lack of information on the wind profile, specifically the upper air across Peninsular Malaysia. Realising the need to establish a standard guideline for national reference, future research, space-aerospace application, and legislation, this study was performed to develop the first wind profile analysis of upper air in Peninsular Malaysia. Relevant data from the Malaysian Meteorology Department was collected for analysis and evaluation. Specifically, a meteorology balloon attached with a sounding radiosonde was used to record data at 0000UTC (0800 h LT) and 1200UTC (2000 h LT) on the 15th day of each month for 7 years (from 2015 to 2021) at the KLIA and Kuantan Meteorology Stations for the wind profiling analysis. The daily overall data collection was recorded accurately once the balloon's rising rate stabilises from vertical air current (katabatic or anabatic winds). Subsequently, the collected data were evaluated in terms of the minimum, maximum, and average wind speeds for each year and time. Finally, the average wind speed of each year and time were combined to generate the Peninsular Malaysia wind profile. Based on the results, the projected wind profile for both stations identified three peaks of discrete sine wave flow type with low-speed wind profile in Peninsular Malaysia. The three peaks amplified the highest air velocity, whereby the 1st and 2nd peaks were located at the troposphere layer from 9,000 m to 12,000 m altitude (average wind speed of 10.8 ms-1) and 12,000 m to 18,000 m altitude (average wind speed of 13.7 ms-1). The 3rd peak was located at the stratosphere layer from 18,000 m to 32,000 m altitude (average wind speed of 15.2 ms-1). Since East Malaysia is located on the same equatorial line, the wind profile is hypothetically the same and exhibits only slight differences. In short, the established wind profile of upper air in Peninsular Malaysia in this study would facilitate other future studies and assist long-term planning of Malaysia's airspace legislation.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140360256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.17576/jsm-2024-5303-04
Sri Wahyuni, Nurwulan Agustiani, I. A. Rumanti, Swisci Margaret, Selly Salma, S. D. Ardhiyanti
In an effort to address labor scarcity and enhance the cropping index in tidal swamp areas, farmers utilize direct seeding techniques. However, flooded conditions due to tides and ebb can hinder seed germination. This study aimed to obtain the best invigoration treatment for improving seedling establishment and growth in South Sumatra's tidal swamp hotspots. In the first part of the study, 19 different seed treatments were tested on the swamp rice variety, Inpara 9, in a greenhouse. These treatments included hardening, priming, thermal treatment, and controls. Soluble sugar content and α-amylase activities of treated seeds were analyzed in the lab. The results showed that the invigorated seeds outperformed the control group in terms of seedling growth and development when they were planted directly in the ground under both anaerobic germination (AG) and anaerobic germination with extra FeSO4 added to the soil (AG+Fe) conditions. All treated seeds also exhibited higher soluble sugar and α-amylase activities. A follow-up experiment in a hotspot area assessed the effectiveness of the top nine seed treatments from the first experiment under AG conditions. The flooded level was managed at around 10 cm during the experiment and Inpara-9 was the variety planted. All invigorated seeds exhibited superior seedling establishment and growth compared to the control on in-situ swampy land. Hardening using Trichoderma yielded the highest seedling establishment and performance, indicated by greater root and shoot dry weight than other treatments, followed by hardening with Methylobacterium td-J7 and Methylobacterium td-tpb3.
{"title":"Enhancing Seedling Performance Direct Seeded Rice Under Anaerobic Germination Condition by Seed Invigoration","authors":"Sri Wahyuni, Nurwulan Agustiani, I. A. Rumanti, Swisci Margaret, Selly Salma, S. D. Ardhiyanti","doi":"10.17576/jsm-2024-5303-04","DOIUrl":"https://doi.org/10.17576/jsm-2024-5303-04","url":null,"abstract":"In an effort to address labor scarcity and enhance the cropping index in tidal swamp areas, farmers utilize direct seeding techniques. However, flooded conditions due to tides and ebb can hinder seed germination. This study aimed to obtain the best invigoration treatment for improving seedling establishment and growth in South Sumatra's tidal swamp hotspots. In the first part of the study, 19 different seed treatments were tested on the swamp rice variety, Inpara 9, in a greenhouse. These treatments included hardening, priming, thermal treatment, and controls. Soluble sugar content and α-amylase activities of treated seeds were analyzed in the lab. The results showed that the invigorated seeds outperformed the control group in terms of seedling growth and development when they were planted directly in the ground under both anaerobic germination (AG) and anaerobic germination with extra FeSO4 added to the soil (AG+Fe) conditions. All treated seeds also exhibited higher soluble sugar and α-amylase activities. A follow-up experiment in a hotspot area assessed the effectiveness of the top nine seed treatments from the first experiment under AG conditions. The flooded level was managed at around 10 cm during the experiment and Inpara-9 was the variety planted. All invigorated seeds exhibited superior seedling establishment and growth compared to the control on in-situ swampy land. Hardening using Trichoderma yielded the highest seedling establishment and performance, indicated by greater root and shoot dry weight than other treatments, followed by hardening with Methylobacterium td-J7 and Methylobacterium td-tpb3.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140361083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.17576/jsm-2024-5303-05
Mara Jean Marielle Calapardo, B. Manigo
The present method of stevia propagation is still insufficient to meet its increasing demand. Moreover, the use of organic inputs as substitutes for inorganic fertilizers to improve soil quality and productivity is also desired. In this study, the effects of bio-organic nutrients and different growing media compositions on Stevia’s herbage growth, yield and quality were determined. A completely randomized design was used with 20 rooted stem cuttings per replication at one cutting per potting bag, laid in a 4 × 4 factorial arrangement. Growing Media (garden soil alone [GS]; GS + rice hull [RH]; GS + vermicompost [VC]; and GS+RH+VC) served as factor A while bio-organic nutrients (fermented fruit juice [FFJ]; vermi tea [VT]; seaweed tea [ST] and control) as factor B. There were 16 treatment combinations replicated four times. Results showed that GS+RH+VC consistently improved the survival rate, plant height, leaf and shoot count per plant, weight (fresh and dry), and herbage yield of Stevia. Furthermore, VT boosted the herbage yield and visual quality. In terms of interaction effects, plants grown in garden soil mixed with vermicompost (GS+VC) with VT application developed more leaves than other treatment combinations. Hence, these bio-nutrients and growing media composition significantly enhanced the production of Stevia.
目前的甜叶菊繁殖方法仍不足以满足日益增长的需求。此外，人们还希望使用有机肥料替代无机肥料，以提高土壤质量和生产力。本研究确定了生物有机养分和不同生长介质成分对甜叶菊草本植物生长、产量和质量的影响。采用完全随机设计，每个重复 20 根茎插条，每个盆栽袋一根插条，按 4 × 4 因式排列。生长介质（园土[GS]、GS + 稻壳[RH]、GS + 蛭石堆肥[VC]和 GS+RH+VC）为 A 因子，生物有机养分（发酵果汁[FFJ]、蚯蚓茶[VT]、海藻茶[ST]和对照）为 B 因子。结果表明，GS+RH+VC 能持续提高甜叶菊的成活率、株高、单株叶片和嫩枝数、重量（鲜重和干重）和草本产量。此外，VT 还提高了药草产量和视觉质量。在交互效应方面，与其他处理组合相比，在施用了 VT 的园土与蛭石堆肥（GS+VC）中生长的植物长出了更多的叶子。因此，这些生物营养素和生长介质成分显著提高了甜叶菊的产量。
{"title":"Enhancing Herbage Growth, Yield and Quality of Stevia (Stevia rebaudiana Bertoni) using Bio-Organic Nutrients in Varied Soil Media","authors":"Mara Jean Marielle Calapardo, B. Manigo","doi":"10.17576/jsm-2024-5303-05","DOIUrl":"https://doi.org/10.17576/jsm-2024-5303-05","url":null,"abstract":"The present method of stevia propagation is still insufficient to meet its increasing demand. Moreover, the use of organic inputs as substitutes for inorganic fertilizers to improve soil quality and productivity is also desired. In this study, the effects of bio-organic nutrients and different growing media compositions on Stevia’s herbage growth, yield and quality were determined. A completely randomized design was used with 20 rooted stem cuttings per replication at one cutting per potting bag, laid in a 4 × 4 factorial arrangement. Growing Media (garden soil alone [GS]; GS + rice hull [RH]; GS + vermicompost [VC]; and GS+RH+VC) served as factor A while bio-organic nutrients (fermented fruit juice [FFJ]; vermi tea [VT]; seaweed tea [ST] and control) as factor B. There were 16 treatment combinations replicated four times. Results showed that GS+RH+VC consistently improved the survival rate, plant height, leaf and shoot count per plant, weight (fresh and dry), and herbage yield of Stevia. Furthermore, VT boosted the herbage yield and visual quality. In terms of interaction effects, plants grown in garden soil mixed with vermicompost (GS+VC) with VT application developed more leaves than other treatment combinations. Hence, these bio-nutrients and growing media composition significantly enhanced the production of Stevia.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140358557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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