Pub Date : 2022-01-01DOI: 10.15258/sst.2022.50.2.11
Marina Remião Dos Santos, G. Schlindwein, A. Tonietto, Juliano Morales de Oliveira
Butia yatay (Arecaceae) is a threatened palm that occurs naturally in native grasslands of southern Brazil. Its fruits are an alternative source of income for the local populations with potential for agro-industry. This generates a demand for seedlings that is limited by the difficult propagation due to the dormancy of the seeds. Thus, this work aimed to develop a method capable of accelerating germination by overcoming dormancy. Pyrenes from four inflorescences were harvested from different trees in the municipality of Três de Maio/Brazil. In the first experiment, the pyrenes were warm-stratified at 40°C for 0 (control), 1, 2, 3 or 4 weeks. In experiment 2, pyrenes scarified manually (removal of the endocarp germ pore) or using H2SO4 for 5 or 10 minutes were subsequently warm-stratified for four weeks. In experiment 3, pyrenes subjected to physical scarification were warm-stratified for 4, 5, 6 or 8 weeks. Analyses showed that there was a significant effect of the preheating and scarification treatments on the emergence of B. yatay seedlings. Pyrenes mechanically scarified (opening of the germ pore) and subsequently subjected to preheating at 40°C for five weeks produced the highest speed of emergence index, with about 28 seedlings emerged from 100 sown pyrenes.
Butia yatay(槟榔科)是一种受威胁的棕榈，自然生长在巴西南部的原生草原上。它的果实是当地人口的另一种收入来源，具有农业工业的潜力。这就产生了对幼苗的需求，但由于种子休眠而难以繁殖，因此对幼苗的需求受到限制。因此，这项工作旨在开发一种能够通过克服休眠来加速发芽的方法。从巴西Três de Maio市的不同树木上收获了四个花序的比利牛斯。在第一个实验中，将芘在40°C下进行0(对照)、1、2、3或4周的温分层。在实验2中，手工切割(去除内胚芽孔)或使用H2SO4加热5或10分钟的芘，然后进行4周的温分层。在实验3中，经物理划伤的芘分别进行4、5、6、8周的温分层处理。分析表明，预处理和刻蚀处理对青玉苗出苗有显著影响。机械绞杀(打开胚芽孔)和随后在40°C下预热5周的比利牛的出苗速度指数最高，100株播种的比利牛中约有28株出苗。
{"title":"Dormancy release in diaspores of Butia yatay (Arecaceae) using scarification and warm stratification","authors":"Marina Remião Dos Santos, G. Schlindwein, A. Tonietto, Juliano Morales de Oliveira","doi":"10.15258/sst.2022.50.2.11","DOIUrl":"https://doi.org/10.15258/sst.2022.50.2.11","url":null,"abstract":"Butia yatay (Arecaceae) is a threatened palm that occurs naturally in native grasslands of southern Brazil. Its fruits are an alternative source of income for the local populations with potential for agro-industry. This generates a demand for seedlings that is limited by the difficult propagation due to the dormancy of the seeds. Thus, this work aimed to develop a method capable of accelerating germination by overcoming dormancy. Pyrenes from four inflorescences were harvested from different trees in the municipality of Três de Maio/Brazil. In the first experiment, the pyrenes were warm-stratified at 40°C for 0 (control), 1, 2, 3 or 4 weeks. In experiment 2, pyrenes scarified manually (removal of the endocarp germ pore) or using H2SO4 for 5 or 10 minutes were subsequently warm-stratified for four weeks. In experiment 3, pyrenes subjected to physical scarification were warm-stratified for 4, 5, 6 or 8 weeks. Analyses showed that there was a significant effect of the preheating and scarification treatments on the emergence of B. yatay seedlings. Pyrenes mechanically scarified (opening of the germ pore) and subsequently subjected to preheating at 40°C for five weeks produced the highest speed of emergence index, with about 28 seedlings emerged from 100 sown pyrenes.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.15258/sst.2022.50.1.09
K. Whitehouse, S. Norton
Orthodox seed longevity systematically increases with reduction in temperature and moisture content, as described by the Ellis and Roberts viability equations. These equations have species-specific parameters which quantify the inherent longevity of seeds of a particular species and their sensitivity to change in moisture content. This research investigated the effect of moisture content on lentil (Lens culinaris) seed longevity during experimental storage. Seeds of an elite breeding line were stored under hermetic conditions at six different moisture contents (7.6, 8.1, 8.8, 10.1, 11.5, 12.6%) at 60°C. Ability to germinate was determined at scheduled intervals, and longevity (σ) was estimated for each treatment. The values of the constants KE (8.0008) and CW (3.746) were estimated using linear regression, by plotting log σ against log moisture content, combined with the universal temperature constants (CH = 0.0329, CQ = 0.000478). The application of these moisture constants (KE and CW) was tested by comparing the predicted viability loss of two lentil accessions with their observed longevity under hermetic storage at 12.0% moisture content and 45°C. The validity of these constants for predicting lentil seed longevity was proven, with an R2 = 0.912 (P < 0.001).
{"title":"Estimation of seed viability constants (KE, CW) for lentil (Lens culinaris)","authors":"K. Whitehouse, S. Norton","doi":"10.15258/sst.2022.50.1.09","DOIUrl":"https://doi.org/10.15258/sst.2022.50.1.09","url":null,"abstract":"Orthodox seed longevity systematically increases with reduction in temperature and moisture content, as described by the Ellis and Roberts viability equations. These equations have species-specific parameters which quantify the inherent longevity of seeds of a particular species and their sensitivity to change in moisture content. This research investigated the effect of moisture content on lentil (Lens culinaris) seed longevity during experimental storage. Seeds of an elite breeding line were stored under hermetic conditions at six different moisture contents (7.6, 8.1, 8.8, 10.1, 11.5, 12.6%) at 60°C. Ability to germinate was determined at scheduled intervals, and longevity (σ) was estimated for each treatment. The values of the constants KE (8.0008) and CW (3.746) were estimated using linear regression, by plotting log σ against log moisture content, combined with the universal temperature constants (CH = 0.0329, CQ = 0.000478). The application of these moisture constants (KE and CW) was tested by comparing the predicted viability loss of two lentil accessions with their observed longevity under hermetic storage at 12.0% moisture content and 45°C. The validity of these constants for predicting lentil seed longevity was proven, with an R2 = 0.912 (P < 0.001).","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67090753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-31DOI: 10.15258/sst.2021.49.2.04
Yingchun Su, Guorui Fan, Jiahui Sun, Linmao Zhao, Chunqing Zhang
On-farm seed priming, which refers to soaking seeds in water, is a pre-sowing seed enhancement technique in agriculture production. This study aimed to investigate the effects of on-farm priming on the germination performance of maize seeds with different vigour levels. Two maize hybrids and four inbred lines were used as experimental materials. Soaking treatment significantly decreased the final germination percentage of low- vigour seeds and had no beneficial effects on high-vigour seeds. With longer soaking times, the reduction in germination increased and there was a positive, negative or neutral effect of water soaking on the germination index. The responses of seeds to on-farm priming varied among the different hybrid / inbred lines used and depending on the length of the treatment. Reducing imbibition damage by PEG soaking had little impact on the poor germination induced by soaking in water. Removal of the seed pericarp alleviated the negative effects of soaking on germination. This study has demonstrated, for the first time, the differential effects of water soaking on maize seeds with different vigour levels. Our results suggested that all influencing factors, such as such as seed initial physiological quality and the technological process, need to be considered to realize the full potential of priming.
{"title":"Germination characteristics of maize seeds with high and low- vigour levels in response to on-farm seed priming","authors":"Yingchun Su, Guorui Fan, Jiahui Sun, Linmao Zhao, Chunqing Zhang","doi":"10.15258/sst.2021.49.2.04","DOIUrl":"https://doi.org/10.15258/sst.2021.49.2.04","url":null,"abstract":"On-farm seed priming, which refers to soaking seeds in water, is a pre-sowing seed enhancement technique in agriculture production. This study aimed to investigate the effects of on-farm priming on the germination performance of maize seeds with different vigour levels. Two maize hybrids\u0000 and four inbred lines were used as experimental materials. Soaking treatment significantly decreased the final germination percentage of low- vigour seeds and had no beneficial effects on high-vigour seeds. With longer soaking times, the reduction in germination increased and there was a positive,\u0000 negative or neutral effect of water soaking on the germination index. The responses of seeds to on-farm priming varied among the different hybrid / inbred lines used and depending on the length of the treatment. Reducing imbibition damage by PEG soaking had little impact on the poor germination\u0000 induced by soaking in water. Removal of the seed pericarp alleviated the negative effects of soaking on germination. This study has demonstrated, for the first time, the differential effects of water soaking on maize seeds with different vigour levels. Our results suggested that all influencing\u0000 factors, such as such as seed initial physiological quality and the technological process, need to be considered to realize the full potential of priming.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46988019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-31DOI: 10.15258/sst.2021.49.2.06
Kantima Thongsri, Kanokwan Teingtham, J. Duangpatra, J. Romkaew
Low temperature has negative effects on seed performance and seedling growth in soya bean, reducing stand establishment. It was hypothesised, that seed treatment with plant hormones could improve seed imbibition and performance at low temperature. Therefore, the effects of 24-epibrassinolide (EBL) and gibberellic acid (GA3) on soya bean seed germination were studied. Seeds of 'CM60' and 'CM0701-24' were imbibed with 0.25 or 0.50 ppm EBL, 50 or 100 ppm GA3, or distilled water at 10, 15, 20 and 25°C. The seed imbibition patterns of the two soya bean varieties followed a similar trend. Low temperature delayed seed imbibition resulting in longer time to reach the end of phases 1 and 2 of seed germination as compared with high temperature. Soya bean seeds treated with 50 or 100 ppm GA3 showed increased germination index, shoot and root length and reduced mean germination time. Thus, 50-100 ppm GA3 could be used as a pre-soaking seed treatment to improve soya bean seed and seedling performance under low temperature.
{"title":"Effects of brassinosteroids and gibberellin on water uptake and performance of soya bean seeds under different temperatures","authors":"Kantima Thongsri, Kanokwan Teingtham, J. Duangpatra, J. Romkaew","doi":"10.15258/sst.2021.49.2.06","DOIUrl":"https://doi.org/10.15258/sst.2021.49.2.06","url":null,"abstract":"Low temperature has negative effects on seed performance and seedling growth in soya bean, reducing stand establishment. It was hypothesised, that seed treatment with plant hormones could improve seed imbibition and performance at low temperature. Therefore, the effects of 24-epibrassinolide\u0000 (EBL) and gibberellic acid (GA3) on soya bean seed germination were studied. Seeds of 'CM60' and 'CM0701-24' were imbibed with 0.25 or 0.50 ppm EBL, 50 or 100 ppm GA3, or distilled water at 10, 15, 20 and 25°C. The seed imbibition patterns of the two soya bean varieties\u0000 followed a similar trend. Low temperature delayed seed imbibition resulting in longer time to reach the end of phases 1 and 2 of seed germination as compared with high temperature. Soya bean seeds treated with 50 or 100 ppm GA3 showed increased germination index, shoot and root\u0000 length and reduced mean germination time. Thus, 50-100 ppm GA3 could be used as a pre-soaking seed treatment to improve soya bean seed and seedling performance under low temperature.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42181804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-31DOI: 10.15258/sst.2021.49.2.07
Alessandro Romano, Romana Bravi
Water deficit is one of the main constraints of germination for many cultivated species. Among the techniques used to promote germination, seeds priming has been found to play a key role. In the present study, a set of different priming agents (KCl, KH2PO4, CaCl2 and PEG 8000) was tested on seeds of two leek (Allium ampeloprasum L.) cultivars ("Lungo della riviera" and "Monstrueux de Carentan") to assess the effects on germination performances both in optimal hydration condition (Ψw = 0 MPa) and under water deficit (–0.3 and –0.6 MPa). Germination percentage of "Lungo della riviera" was improved by PEG and KCl at –0.3 MPa and –0.6 MPa, respectively, whereas CaCl2 improved germination of "Monstrueux de Carentan" at –0.6 MPa. Osmopriming was beneficial in terms of germination rate although to different extent depending on genotype and priming agent. The hydrotime model showed that the effects of priming on germination rate was mostly due to a lowering of the predicted hydrotime constant (θH), whereas median base water potential Ψb (50) tended to increase following treatments albeit with some exception. In this study, hydrotime model proved to be a useful tool to test the effectiveness of different priming treatments on leek seeds and the intraspecific variability in germination capability and speed under water stress.
水分亏缺是许多栽培植物发芽的主要制约因素之一。在用于促进发芽的技术中，种子激发已被发现起着关键作用。以两种韭菜(Allium ampeloprasum L.)品种“Lungo della riviera”和“Monstrueux de Carentan”的种子为试验材料，采用KCl、KH2PO4、CaCl2和peg8000 4种不同的激发剂，研究了最佳水化条件(Ψw = 0 MPa)和水分亏缺条件(-0.3和-0.6 MPa)对种子萌发性能的影响。PEG和KCl分别在-0.3 MPa和-0.6 MPa下提高了“Lungo della riviera”的发芽率，而CaCl2在-0.6 MPa下提高了“Monstrueux de Carentan”的发芽率。浸渍对种子的发芽率有显著的促进作用，但因基因型和浸渍剂的不同而有不同的促进作用。水时间模型表明，淹水处理对发芽率的影响主要是由于预测水时间常数(θH)的降低，而中位碱水势Ψb(50)在处理后有增加的趋势，但也有例外。在本研究中，水时间模型被证明是一个有用的工具来测试不同的启动处理对韭菜种子的有效性和在水分胁迫下萌发能力和速度的种内变异性。
{"title":"Hydrotime model to evaluate the effects of a set of priming agents on seed germination of two leek cultivars under water stress","authors":"Alessandro Romano, Romana Bravi","doi":"10.15258/sst.2021.49.2.07","DOIUrl":"https://doi.org/10.15258/sst.2021.49.2.07","url":null,"abstract":"Water deficit is one of the main constraints of germination for many cultivated species. Among the techniques used to promote germination, seeds priming has been found to play a key role. In the present study, a set of different priming agents (KCl, KH2PO4, CaCl2\u0000 and PEG 8000) was tested on seeds of two leek (Allium ampeloprasum L.) cultivars (\"Lungo della riviera\" and \"Monstrueux de Carentan\") to assess the effects on germination performances both in optimal hydration condition (Ψw = 0 MPa) and under water deficit (–0.3\u0000 and –0.6 MPa). Germination percentage of \"Lungo della riviera\" was improved by PEG and KCl at –0.3 MPa and –0.6 MPa, respectively, whereas CaCl2 improved germination of \"Monstrueux de Carentan\" at –0.6 MPa. Osmopriming was beneficial in terms of germination\u0000 rate although to different extent depending on genotype and priming agent. The hydrotime model showed that the effects of priming on germination rate was mostly due to a lowering of the predicted hydrotime constant (θH), whereas median base water potential Ψb\u0000 (50) tended to increase following treatments albeit with some exception. In this study, hydrotime model proved to be a useful tool to test the effectiveness of different priming treatments on leek seeds and the intraspecific variability in germination capability and speed under water stress.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41876994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-31DOI: 10.15258/sst.2021.49.2.05
Botovao Auguste Ramiandrisoa, Cyrille Maharombaka, M. Vorontsova, Hery Lisy Tiana Ranarijaona
Madagascar is home to a diversity of aquatic grasses. One of these is a rare rice relative, Leersia perrieri (known as Ahitsiriry or Tsingirifiry in the Malagasy language), which is found in a restricted area around the Mahajanga wetlands. A germination test was carried out on two types of local substrate and a control with randomised selected seeds. Seed germination on the control and clay substrates reached 50% while on the organic substrate germination failed to reach 40% during 22 days of observation. The GLM analysis of the final germination showed significant differences between the three treatments. The germination percentage (GP%) was higher in the control and significantly decreased on the clay and organic substrates. Our results help explains our field observations where L. perrieri tends to form monodominant stands on clay substrate, and dispersed multispecies assemblages with fewer individuals on organic soils. This work will be of direct benefit to our panned conservation activities and eventually germplasm availability for crop improvement research.
{"title":"Seed germination of Leersia perrieri (Poaceae), a rare crop wild relative from Madagascar","authors":"Botovao Auguste Ramiandrisoa, Cyrille Maharombaka, M. Vorontsova, Hery Lisy Tiana Ranarijaona","doi":"10.15258/sst.2021.49.2.05","DOIUrl":"https://doi.org/10.15258/sst.2021.49.2.05","url":null,"abstract":"Madagascar is home to a diversity of aquatic grasses. One of these is a rare rice relative, Leersia perrieri (known as Ahitsiriry or Tsingirifiry in the Malagasy language), which is found in a restricted area around the Mahajanga wetlands. A germination test was carried out on\u0000 two types of local substrate and a control with randomised selected seeds. Seed germination on the control and clay substrates reached 50% while on the organic substrate germination failed to reach 40% during 22 days of observation. The GLM analysis of the final germination showed significant\u0000 differences between the three treatments. The germination percentage (GP%) was higher in the control and significantly decreased on the clay and organic substrates. Our results help explains our field observations where L. perrieri tends to form monodominant stands on clay substrate,\u0000 and dispersed multispecies assemblages with fewer individuals on organic soils. This work will be of direct benefit to our panned conservation activities and eventually germplasm availability for crop improvement research.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49037843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seeds of Impatiens hainanensis were stored in three different environments: ambient (25°C, 70-80% RH), dry (25°C, 2-5% RH) and cold (4°C, 40-50% RH). The seeds maintained their germination for 90 days in the three storage conditions, albeit at relatively low levels, particularly for seeds in cold storage. Seed vigour was maintained for 45 days for seeds in cold storage, 60 days for seeds in ambient storage, and 90 days for seeds in dry storage. A moisture content around 3.8% is suggested as appropriate for the storage of I. hainanensis seeds. During storage (up to 90 days) under ambient and cold storage conditions, soluble sugar content, SOD and CAT activity all decreased, and were lower than seeds in dry storage. Relative conductivity and TBARS content both increased during ambient and cold storage and were higher than seeds in dry storage after the same storage period. Dry storage better maintained the antioxidant enzyme system of I. hainanensis seeds.
{"title":"Storage behaviour of Impatiens hainanensis seeds stored under three conditions","authors":"Weixia Huang, Yunfang Zhong, Xinya Meng, Xiqiang Song, Ming-Xun Ren, Yanjun Du","doi":"10.15258/sst.2021.49.2.08","DOIUrl":"https://doi.org/10.15258/sst.2021.49.2.08","url":null,"abstract":"Seeds of Impatiens hainanensis were stored in three different environments: ambient (25°C, 70-80% RH), dry (25°C, 2-5% RH) and cold (4°C, 40-50% RH). The seeds maintained their germination for 90 days in the three storage conditions, albeit at relatively low levels,\u0000 particularly for seeds in cold storage. Seed vigour was maintained for 45 days for seeds in cold storage, 60 days for seeds in ambient storage, and 90 days for seeds in dry storage. A moisture content around 3.8% is suggested as appropriate for the storage of I. hainanensis seeds. During\u0000 storage (up to 90 days) under ambient and cold storage conditions, soluble sugar content, SOD and CAT activity all decreased, and were lower than seeds in dry storage. Relative conductivity and TBARS content both increased during ambient and cold storage and were higher than seeds in dry storage\u0000 after the same storage period. Dry storage better maintained the antioxidant enzyme system of I. hainanensis seeds.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46610433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-23DOI: 10.21203/rs.3.rs-810603/v1
Liwen Su, Lian-Ju Ma, Xiaochun Huang, Wenting Wu, Hai-Hua Lv, Zhikui Cheng, J. Gou, Yong Chen, Peng Wang, Zhengguo Liu
Bitter gourd (Momordica charantia L.) is one of the most important vegetable crops in many Asian and African countries. Here, InDel markers developed by comparing whole genome re-sequencing data were used to analyze the genetic diversity of a bitter gourd germplasm sample from various geographical origins. To verify the reliability of the set of InDel markers identified, 220 pairs of InDel primers were designed. The primers were preliminarily detected by 8% polyacrylamide gel electrophoresis, and 25 pairs of primers with better polymorphism were screened. Using the 25 primer combinations, the 53 bitter gourd accessions were effectively distinguished and the InDel fingerprint of DNA was constructed. Concomitantly, the degree of purity of different crosses was determined based on the differences in specific bands among genotypes. The unweighted pair-group method with arithmetic means showed that the 53 bitter gourd materials may be divided into three groups, with a similarity coefficient of 0.645 as the threshold. Therefore, this study can provide many InDel markers for genotypic identification, genetic relationship analysis, and genetic map construction of bitter gourd.
{"title":"Development of InDel Markers and Fingerprinting of Bitter Gourd (Momordica Charantia) Based on Whole Genome Re-sequencing","authors":"Liwen Su, Lian-Ju Ma, Xiaochun Huang, Wenting Wu, Hai-Hua Lv, Zhikui Cheng, J. Gou, Yong Chen, Peng Wang, Zhengguo Liu","doi":"10.21203/rs.3.rs-810603/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-810603/v1","url":null,"abstract":"\u0000 Bitter gourd (Momordica charantia L.) is one of the most important vegetable crops in many Asian and African countries. Here, InDel markers developed by comparing whole genome re-sequencing data were used to analyze the genetic diversity of a bitter gourd germplasm sample from various geographical origins. To verify the reliability of the set of InDel markers identified, 220 pairs of InDel primers were designed. The primers were preliminarily detected by 8% polyacrylamide gel electrophoresis, and 25 pairs of primers with better polymorphism were screened. Using the 25 primer combinations, the 53 bitter gourd accessions were effectively distinguished and the InDel fingerprint of DNA was constructed. Concomitantly, the degree of purity of different crosses was determined based on the differences in specific bands among genotypes. The unweighted pair-group method with arithmetic means showed that the 53 bitter gourd materials may be divided into three groups, with a similarity coefficient of 0.645 as the threshold. Therefore, this study can provide many InDel markers for genotypic identification, genetic relationship analysis, and genetic map construction of bitter gourd.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42429134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-20DOI: 10.15258/SST.2021.49.1.01
Eda Burcu Kucukhuseyin, N. Memi̇ş, Z. Gokdas, S. Groot, I. Demir
Ethanol release was tested in relation to seed vigour measured by seedling emergence and controlled deterioration tolerance in radish, pepper, watermelon, aubergine, leek and onion seed lots. Ethanol release, measured with six lots from each species after four and eight hours at 40°C and 30% seed moisture, was highly negatively correlated with controlled deterioration tolerance (45°C, 48 hours, 20% seed moisture) in radish (r = 0.05, P < 0.05), with seedling emergence in watermelon (r = 0.99, P < 0.01) and to both traits in pepper (r = 0.87 to 0.94, P < 0.05), but not with any variable for aubergine and leek. One single case was significant between ethanol release after eight hours and controlled deterioration for onion seeds. Results indicate that there is a relationship between ethanol release and seed vigour, but this appears to be species dependent.
以萝卜、辣椒、西瓜、茄子、韭菜和洋葱种子批为试验材料，研究了乙醇释放量与种子活力的关系。在40°C、30%种子水分条件下，分别用6个批次测定4 h和8 h的乙醇释放量，结果表明，乙醇释放量与萝卜(45°C、48 h、20%种子水分条件下)、西瓜(r = 0.99, P < 0.01)、辣椒(r = 0.87 ~ 0.94, P < 0.05)、茄子和韭菜(r = 0.87 ~ 0.94, P < 0.05)呈高度负相关(r = 0.05, P < 0.05)。洋葱种子8小时后乙醇释放与控制变质之间有显著性差异。结果表明，乙醇释放量与种子活力之间存在关系，但这似乎是种依赖的。
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Pub Date : 2021-01-01DOI: 10.15258/sst.2021.49.2.03
Kittiwan Klarod, A. Dongsansuk, H. Piepho, B. Siri
A lack of nutrients in the seed can be supplemented by seed coating and has the potential to improve germination and seedling growth. In this research, we studied the physiological effect of coating tomato seeds with plant nutrient formulations (PNF) on the dehydrogenase activity during germination and nutrient content of the seedlings. Two tomato seed lots were used, differing in physiological quality: the low quality seed lot had 76% germination and the medium quality seed lot had 87% germination. Seeds were coated with a complete PNF containing N, P, K, Ca, Mg, Fe, B, Mn, Cu and Zn. The PNF was applied at three concentrations, i.e., 1-fold (PF1), 4-fold (PF4) and 16-fold (PF16), and applied with 3% PVP-K90 + 1% PEG 6000 as a polymer. Coating seeds with PNF improved seed germination for both low- and medium-quality seeds. Accelerated ageing tests revealed that PF1 increased germination by 12 and 27% in low- and medium-quality seeds, respectively. All concentrations of PNF increased TDH activity after 48 hours of seed imbibition. Moreover, PF16 decreased root length but increased shoot length. All PNF treatments resulted in higher nutrient content in the seedlings.
{"title":"Seed coating with plant nutrients enhances germination and seedling growth, and promotes total dehydrogenase activity during seed germination in tomato (Lycopersicon esculentum)","authors":"Kittiwan Klarod, A. Dongsansuk, H. Piepho, B. Siri","doi":"10.15258/sst.2021.49.2.03","DOIUrl":"https://doi.org/10.15258/sst.2021.49.2.03","url":null,"abstract":"A lack of nutrients in the seed can be supplemented by seed coating and has the potential to improve germination and seedling growth. In this research, we studied the physiological effect of coating tomato seeds with plant nutrient formulations (PNF) on the dehydrogenase activity during germination and nutrient content of the seedlings. Two tomato seed lots were used, differing in physiological quality: the low quality seed lot had 76% germination and the medium quality seed lot had 87% germination. Seeds were coated with a complete PNF containing N, P, K, Ca, Mg, Fe, B, Mn, Cu and Zn. The PNF was applied at three concentrations, i.e., 1-fold (PF1), 4-fold (PF4) and 16-fold (PF16), and applied with 3% PVP-K90 + 1% PEG 6000 as a polymer. Coating seeds with PNF improved seed germination for both low- and medium-quality seeds. Accelerated ageing tests revealed that PF1 increased germination by 12 and 27% in low- and medium-quality seeds, respectively. All concentrations of PNF increased TDH activity after 48 hours of seed imbibition. Moreover, PF16 decreased root length but increased shoot length. All PNF treatments resulted in higher nutrient content in the seedlings.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67090535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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