Pub Date : 2026-03-18DOI: 10.1016/j.scienta.2026.114753
Shuanggui Geng, Xiaoyang Chen, Gaoyin Wu, Yunjie Hu, Qian Huang, Guanglin Tao, Jing An, Fang Ming, Yang Yang, Yingliang Liu
{"title":"Physiological and transcriptome analyses for revealing the rooting mechanism of Mussaenda anomala, a species with an extremely small population","authors":"Shuanggui Geng, Xiaoyang Chen, Gaoyin Wu, Yunjie Hu, Qian Huang, Guanglin Tao, Jing An, Fang Ming, Yang Yang, Yingliang Liu","doi":"10.1016/j.scienta.2026.114753","DOIUrl":"https://doi.org/10.1016/j.scienta.2026.114753","url":null,"abstract":"","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"60 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147496083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-17DOI: 10.1016/j.scienta.2026.114746
Faisal Zulfiqar, Anam Moosa, Antonio Ferrante, Anastasios Darras, Temoor Ahmed, Sanaullah Jalil, Ibrahim Al-Ashkar, Ayman El Sabagh
{"title":"Retraction notice to “Melatonin seed priming improves growth and physio-biochemical aspects of Zinnia elegans under salt stress” [HORTI 323C (2023) 1–2/112495]","authors":"Faisal Zulfiqar, Anam Moosa, Antonio Ferrante, Anastasios Darras, Temoor Ahmed, Sanaullah Jalil, Ibrahim Al-Ashkar, Ayman El Sabagh","doi":"10.1016/j.scienta.2026.114746","DOIUrl":"https://doi.org/10.1016/j.scienta.2026.114746","url":null,"abstract":"","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"273 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147465873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-17DOI: 10.1016/j.scienta.2026.114739
P. Mignard, C.M. Cantín, L. Castel, A. Pina, J. Gonzalez, P. Errea
Local apple accessions are vital for their adaptability to specific climates and unique organoleptic characteristics, playing a key role in biodiversity conservation and agricultural resilience. This study examined 36 apple genotypes from the CITA collection, including 25 diploid and 11 triploid varieties distributed across five altitudes in northeastern (NE) Spain. The collection encompassed both autochthonous landraces and non-Spanish commercial cultivars. The analysis focused on physicochemical attributes such as fruit firmness, total phenolic content (TPC), and individual sugars. Among these six genotypes (five autochthonous landraces and one commercial cultivar used as reference [‘Gala’]), were present at two different altitudes within the CITA collection and were further evaluated to assess the influence of altitude elevation on these traits. Significant variability in fruit quality was observed, with notable correlations between physicochemical traits. Principal component analysis (PCA) explained 50.2% of the total variance, highlighting genetic variability among the accessions and its impact on fruit traits. The results showed that ploidy (diploid/triploid) and origin (autochthonous/commercial - non-Spanish) are key factors shaping apple characteristics. Higher altitudes were associated with increased TPC in specific accessions, likely due to cooler temperatures and greater UV and sun exposure enhancing phenolic biosynthesis. Fructose content also increased with altitude, reflecting slower fruit development and ripening, while variations in glucose and sucrose indicated the complexity of metabolic responses to both altitude and genetic factors. These findings demonstrate the high biodiversity of apples from NE Spain, particularly in terms of their organoleptic and nutritional qualities. Preserving autochthonous apple resources is essential, and breeding programs could benefit from selecting accessions with desirable biochemical traits for local climatic conditions. Additional research is needed to explore how environmental factors influence the health benefits of apples, especially their bioactive compounds, as well as their agronomic performance.
{"title":"From valley to mountain: Organoleptic and nutritional properties of apple accessions from Northeast Spain","authors":"P. Mignard, C.M. Cantín, L. Castel, A. Pina, J. Gonzalez, P. Errea","doi":"10.1016/j.scienta.2026.114739","DOIUrl":"https://doi.org/10.1016/j.scienta.2026.114739","url":null,"abstract":"Local apple accessions are vital for their adaptability to specific climates and unique organoleptic characteristics, playing a key role in biodiversity conservation and agricultural resilience. This study examined 36 apple genotypes from the CITA collection, including 25 diploid and 11 triploid varieties distributed across five altitudes in northeastern (NE) Spain. The collection encompassed both autochthonous landraces and non-Spanish commercial cultivars. The analysis focused on physicochemical attributes such as fruit firmness, total phenolic content (TPC), and individual sugars. Among these six genotypes (five autochthonous landraces and one commercial cultivar used as reference [‘Gala’]), were present at two different altitudes within the CITA collection and were further evaluated to assess the influence of altitude elevation on these traits. Significant variability in fruit quality was observed, with notable correlations between physicochemical traits. Principal component analysis (PCA) explained 50.2% of the total variance, highlighting genetic variability among the accessions and its impact on fruit traits. The results showed that ploidy (diploid/triploid) and origin (autochthonous/commercial - non-Spanish) are key factors shaping apple characteristics. Higher altitudes were associated with increased TPC in specific accessions, likely due to cooler temperatures and greater UV and sun exposure enhancing phenolic biosynthesis. Fructose content also increased with altitude, reflecting slower fruit development and ripening, while variations in glucose and sucrose indicated the complexity of metabolic responses to both altitude and genetic factors. These findings demonstrate the high biodiversity of apples from NE Spain, particularly in terms of their organoleptic and nutritional qualities. Preserving autochthonous apple resources is essential, and breeding programs could benefit from selecting accessions with desirable biochemical traits for local climatic conditions. Additional research is needed to explore how environmental factors influence the health benefits of apples, especially their bioactive compounds, as well as their agronomic performance.","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"57 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147465874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-16DOI: 10.1016/j.scienta.2026.114748
Tilen Zamljen, Robert Veberic, Mariana Cecilia Grohar
{"title":"Corrigendum to “Impact of salinity and harvest date on the yield, phenolic and volatile contents of thyme (Thymus vulgaris L.), coriander (Coriandrum sativum L.) and dill (Anethum graveolens L.)” [Scientia Horticulturae Volume 352, October 2025, 114445]","authors":"Tilen Zamljen, Robert Veberic, Mariana Cecilia Grohar","doi":"10.1016/j.scienta.2026.114748","DOIUrl":"https://doi.org/10.1016/j.scienta.2026.114748","url":null,"abstract":"","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"234 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147465878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-16DOI: 10.1016/j.scienta.2026.114734
Pengqian Lu, Keke Zhao, Houshan Wang, Weitao Jiang, Yusong Liu, Gongshuai Wang, Mei Wang, Yanfang Wang, Chengmiao Yin, Zhiquan Mao
This study aims to investigate the efficacy of calcium peroxide (CaO₂) at varying concentrations in controlling apple replant disease (ARD) and elucidate its regulatory mechanisms on the soil microenvironment and the growth of M. hupehensis Rehd. seedlings. Through plate antibacterial tests, measurements of mycelial substance metabolism and antioxidant enzyme activity, as well as electron microscopy observations, we evaluated the inhibitory effect of calcium peroxide on F. proliferatum, the main pathogenic fungus causing ARD. Systematic analysis of relevant indicators of replant soil and replant seedlings was conducted using methods such as plate coating counting, fluorescence quantitative analysis, and high-throughput sequencing. The results demonstrate that CaO₂ treatment significantly inhibits the growth of F. proliferatum mycelium, reduces the abundance of harmful fungi such as Fusarium species in soil, and effectively degrades accumulated phenolic compounds. Additionally, CaO₂ treatment significantly enhanced the antioxidant capacity of M. hupehensis Rehd. seedlings, increased superoxide dismutase (SOD) and peroxidase (POD) activity, and promoted the accumulation of both shoot and root biomass. In summary, the appropriate application of calcium peroxide can effectively mitigate the occurrence of ARD, with its mechanism involving improvements in soil microecology and regulation of plant physiological metabolism. This study provides new theoretical support for the use of oxidizing agents to prevent and control fruit tree replant problems, and offers a feasible technical strategy for sustainable soil utilization and ecological restoration during the renewal process of old orchards.
{"title":"The study of calcium peroxide mitigating apple replant disease (ARD)","authors":"Pengqian Lu, Keke Zhao, Houshan Wang, Weitao Jiang, Yusong Liu, Gongshuai Wang, Mei Wang, Yanfang Wang, Chengmiao Yin, Zhiquan Mao","doi":"10.1016/j.scienta.2026.114734","DOIUrl":"https://doi.org/10.1016/j.scienta.2026.114734","url":null,"abstract":"This study aims to investigate the efficacy of calcium peroxide (CaO₂) at varying concentrations in controlling apple replant disease (ARD) and elucidate its regulatory mechanisms on the soil microenvironment and the growth of <ce:italic>M. hupehensis</ce:italic> Rehd. seedlings. Through plate antibacterial tests, measurements of mycelial substance metabolism and antioxidant enzyme activity, as well as electron microscopy observations, we evaluated the inhibitory effect of calcium peroxide on <ce:italic>F. proliferatum</ce:italic>, the main pathogenic fungus causing ARD. Systematic analysis of relevant indicators of replant soil and replant seedlings was conducted using methods such as plate coating counting, fluorescence quantitative analysis, and high-throughput sequencing. The results demonstrate that CaO₂ treatment significantly inhibits the growth of <ce:italic>F. proliferatum</ce:italic> mycelium, reduces the abundance of harmful fungi such as <ce:italic>Fusarium</ce:italic> species in soil, and effectively degrades accumulated phenolic compounds. Additionally, CaO₂ treatment significantly enhanced the antioxidant capacity of <ce:italic>M. hupehensis</ce:italic> Rehd. seedlings, increased superoxide dismutase (SOD) and peroxidase (POD) activity, and promoted the accumulation of both shoot and root biomass. In summary, the appropriate application of calcium peroxide can effectively mitigate the occurrence of ARD, with its mechanism involving improvements in soil microecology and regulation of plant physiological metabolism. This study provides new theoretical support for the use of oxidizing agents to prevent and control fruit tree replant problems, and offers a feasible technical strategy for sustainable soil utilization and ecological restoration during the renewal process of old orchards.","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"6 2 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147465881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-14DOI: 10.1016/j.scienta.2026.114751
Qiuyu Zhao, Bing Hu, Guiyan Chen, Ye Chen, Changming Yang, Lifang Zhang, Bin Yang, Fazhong Yang, Yunxian Li
Rose (Rosa chinensis), as the most popular cut flower in the world, is frequently attacked by rose powdery mildew (Podosphaera pannosa) and beet armyworm (Spodoptera exigua). Indirect plant-mediated interactions between these two pests consistently occur in the ternary system of these three organisms, but little is known about the functions of rose genes of triterpenoid-related key enzymes for the interactions. In this study, we investigated the functional characteristics of the squalene epoxidase (SE) gene, which was upregulated in roses after infection with the mildew. The two SE genes (LOC112173431 and LOC112174075) were cloned from healthy (control) and infected rose leaves, and real-time PCR results showed that these genes were upregulated 29.1-fold and 46.8-fold, respectively, when compared to the controls. Bioinformatic analysis revealed that the conserved domains are critical for squalene binding and catalytic activity, while molecular docking identified key residues involved in substrate interaction (e.g., VAL67, TYR78). Heterologous expression of RcSE1 (LOC112173431) in Escherichia coli confirmed its enzymatic activity with a specific activity of 15.43 IU/L. In Saccharomyces cerevisiae, overexpression of RcSE1 led to a 45% reduction in squalene accumulation, and alongside an increase in the sterol contents. The transgenic Arabidopsis thaliana plants with overexpression ofRcSE1 showed significant increases in the contents of the S.exigua-resistant triterpenoids, including maslinic acid by 46.87 times, betulonic acid by 8.12 times, ganoderic acid B by 1.07 times, and ursolic acid by 2.86 times in the T2 generation, suggesting that RcSE1 can directly activate the metabolic pathway critical for insect resistance without the mildew infection or hormone signals. The results indicate that RcSE1 plays a critical role in the interactions.
{"title":"Functional characterization of squalene epoxidase genes in Rosa chinensis upregulated by infection with Podosphaera pannosa","authors":"Qiuyu Zhao, Bing Hu, Guiyan Chen, Ye Chen, Changming Yang, Lifang Zhang, Bin Yang, Fazhong Yang, Yunxian Li","doi":"10.1016/j.scienta.2026.114751","DOIUrl":"https://doi.org/10.1016/j.scienta.2026.114751","url":null,"abstract":"Rose (<ce:italic>Rosa chinensis</ce:italic>), as the most popular cut flower in the world, is frequently attacked by rose powdery mildew (<ce:italic>Podosphaera pannosa</ce:italic>) and beet armyworm (<ce:italic>Spodoptera exigua</ce:italic>). Indirect plant-mediated interactions between these two pests consistently occur in the ternary system of these three organisms, but little is known about the functions of rose genes of triterpenoid-related key enzymes for the interactions. In this study, we investigated the functional characteristics of the squalene epoxidase (SE) gene, which was upregulated in roses after infection with the mildew. The two SE genes (<ce:italic>LOC112173431</ce:italic> and <ce:italic>LOC112174075</ce:italic>) were cloned from healthy (control) and infected rose leaves, and real-time PCR results showed that these genes were upregulated 29.1-fold and 46.8-fold, respectively, when compared to the controls. Bioinformatic analysis revealed that the conserved domains are critical for squalene binding and catalytic activity, while molecular docking identified key residues involved in substrate interaction (e.g., VAL67, TYR78). Heterologous expression of <ce:italic>RcSE1</ce:italic> (<ce:italic>LOC112173431</ce:italic>) in <ce:italic>Escherichia coli</ce:italic> confirmed its enzymatic activity with a specific activity of 15.43 IU/L. In <ce:italic>Saccharomyces cerevisiae</ce:italic>, overexpression of <ce:italic>RcSE1</ce:italic> led to a 45% reduction in squalene accumulation, and alongside an increase in the sterol contents. The transgenic <ce:italic>Arabidopsis thaliana</ce:italic> plants with overexpression of<ce:italic>RcSE1</ce:italic> showed significant increases in the contents of the <ce:italic>S.exigua</ce:italic>-resistant triterpenoids, including maslinic acid by 46.87 times, betulonic acid by 8.12 times, ganoderic acid B by 1.07 times, and ursolic acid by 2.86 times in the T2 generation, suggesting that <ce:italic>RcSE1</ce:italic> can directly activate the metabolic pathway critical for insect resistance without the mildew infection or hormone signals. The results indicate that <ce:italic>RcSE1</ce:italic> plays a critical role in the interactions.","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"5 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147465880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Noninvasive and accurate quantification of plant architectural traits remains a persistent challenge in horticultural crop breeding and high-throughput phenotyping (HTPP), largely due to labor constraints and limited availability of scalable 3D sensing technologies. Time-of-Flight (ToF) imaging offers a promising active sensing approach capable of generating high-resolution digital replicas of plant canopies for structural trait analysis. This study evaluated the feasibility of deploying 3D ToF imaging for digital phenotyping of three horticultural and ornamental crops, guava, brinjal, and jasmine with a focus on canopy volume estimation. High-resolution 3D point clouds were acquired for five plants per crop across three growth stages during the summer season of 2024. Structural parameters including plant height, width North–South (WNS), width East–West (WEW), and 3D aspect ratio were extracted and used to compute canopy volume via a voxel grid method. To validate accuracy, ToF-derived canopy volumes were compared against manually measured volumes estimated using the prolate spheroid volume (PSV) method. The ToF-based digital phenotyping framework demonstrated strong agreement with manual measurements across all crops. Guava exhibited the highest accuracy at the intermediate growth stage (R² = 1.0; RMSE = 0.0004), while brinjal (R² = 0.908; RMSE = 0.004) and jasmine (R² = 0.935; RMSE = 0.4351) showed robust performance at the full-grown stage. Those regression relationships were statistically significant (p < 0.05), confirming the reliability of ToF-based canopy reconstruction for structural phenotyping. Overall, the findings demonstrate that ToF imaging provides a robust, noninvasive, and scalable framework for digital canopy phenotyping in horticultural crops, supporting precision breeding and structural trait monitoring applications in field environments.
{"title":"Time-of-Flight (ToF) camera technology for high throughput holistic phenotyping and canopy volume measurement of horticultural crops","authors":"Narendra Singh Chandel , Subir Kumar Chakraborty , Abhilash Kumar Chandel , Abhishek Upadhyay , Bhupendra Singh Parmar , Monika Manjhi , Dilip Jat","doi":"10.1016/j.scienta.2026.114742","DOIUrl":"10.1016/j.scienta.2026.114742","url":null,"abstract":"<div><div>Noninvasive and accurate quantification of plant architectural traits remains a persistent challenge in horticultural crop breeding and high-throughput phenotyping (HTPP), largely due to labor constraints and limited availability of scalable 3D sensing technologies. Time-of-Flight (ToF) imaging offers a promising active sensing approach capable of generating high-resolution digital replicas of plant canopies for structural trait analysis. This study evaluated the feasibility of deploying 3D ToF imaging for digital phenotyping of three horticultural and ornamental crops, guava, brinjal, and jasmine with a focus on canopy volume estimation. High-resolution 3D point clouds were acquired for five plants per crop across three growth stages during the summer season of 2024. Structural parameters including plant height, width North–South (W<sub>NS</sub>), width East–West (W<sub>EW</sub>), and 3D aspect ratio were extracted and used to compute canopy volume via a voxel grid method. To validate accuracy, ToF-derived canopy volumes were compared against manually measured volumes estimated using the prolate spheroid volume (PSV) method. The ToF-based digital phenotyping framework demonstrated strong agreement with manual measurements across all crops. Guava exhibited the highest accuracy at the intermediate growth stage (R² = 1.0; RMSE = 0.0004), while brinjal (R² = 0.908; RMSE = 0.004) and jasmine (R² = 0.935; RMSE = 0.4351) showed robust performance at the full-grown stage. Those regression relationships were statistically significant (<em>p</em> < 0.05), confirming the reliability of ToF-based canopy reconstruction for structural phenotyping. Overall, the findings demonstrate that ToF imaging provides a robust, noninvasive, and scalable framework for digital canopy phenotyping in horticultural crops, supporting precision breeding and structural trait monitoring applications in field environments.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"358 ","pages":"Article 114742"},"PeriodicalIF":4.2,"publicationDate":"2026-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147425032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-15Epub Date: 2026-02-23DOI: 10.1016/j.scienta.2026.114700
Ping Zheng , Haichao Gong , Denglin Li , Sitong Hu , Ludan Zhang , Jing Wang , Lulu Wang , Yuqin Liang , Biao Deng , Kaichuang Liu , Xiaomei Wang , Yuan Qin
Fruit flesh translucency is a major physiological disorder that severely compromises pineapple quality, yet its molecular basis remains insufficiently understood. To address this gap, we performed integrated transcriptomic and metabolomic analyses comparing translucent and healthy fruits of the cultivar ‘MD2’. Transcriptomic and metabolic reprogramming revealed that sugar transporters were markedly down-regulated, accompanied by enhanced glycolysis, organic acid turnover, and amino acid biosynthesis, leading to local accumulation of soluble sugars and polyols and increased osmotic potential. Coordinated cell wall degradation, membrane phospholipid remodeling, and reduced aquaporin expression impaired water homeostasis, thereby promoting extracellular fluid accumulation. Concurrent activation of antioxidant defense and stress-responsive transcription factors indicated intensified oxidative stress, while brassinosteroid, jasmonate, and calcium signaling appeared to modulate these metabolic and structural responses. Collectively, these findings provide a comprehensive molecular framework for understanding pineapple translucency and offer potential molecular targets and practical insights that could inform breeding and management strategies to mitigate this disorder and improve fruit quality.
{"title":"Multi-omics dissection of molecular and metabolic drivers of pineapple fruit flesh translucency","authors":"Ping Zheng , Haichao Gong , Denglin Li , Sitong Hu , Ludan Zhang , Jing Wang , Lulu Wang , Yuqin Liang , Biao Deng , Kaichuang Liu , Xiaomei Wang , Yuan Qin","doi":"10.1016/j.scienta.2026.114700","DOIUrl":"10.1016/j.scienta.2026.114700","url":null,"abstract":"<div><div>Fruit flesh translucency is a major physiological disorder that severely compromises pineapple quality, yet its molecular basis remains insufficiently understood. To address this gap, we performed integrated transcriptomic and metabolomic analyses comparing translucent and healthy fruits of the cultivar ‘MD2’. Transcriptomic and metabolic reprogramming revealed that sugar transporters were markedly down-regulated, accompanied by enhanced glycolysis, organic acid turnover, and amino acid biosynthesis, leading to local accumulation of soluble sugars and polyols and increased osmotic potential. Coordinated cell wall degradation, membrane phospholipid remodeling, and reduced aquaporin expression impaired water homeostasis, thereby promoting extracellular fluid accumulation. Concurrent activation of antioxidant defense and stress-responsive transcription factors indicated intensified oxidative stress, while brassinosteroid, jasmonate, and calcium signaling appeared to modulate these metabolic and structural responses. Collectively, these findings provide a comprehensive molecular framework for understanding pineapple translucency and offer potential molecular targets and practical insights that could inform breeding and management strategies to mitigate this disorder and improve fruit quality.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"358 ","pages":"Article 114700"},"PeriodicalIF":4.2,"publicationDate":"2026-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147278338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-15Epub Date: 2026-02-24DOI: 10.1016/j.scienta.2026.114711
Zhengxiu Ye, Yonghong Zhang, Lanlan Zheng, Chen Li, Lei You, Xiangyin Luo
The young shoots of Zingiber striolatum Diels are considered as a special vegetable in China. Shading cultivation is a key practice to improve the taste and quality of Z. striolatum shoot. However, the underlying mechanism remains poorly understood. In this study, we performed combined transcriptomic and metabolomic analyses of light and shade cultivated Z. striolatum shoot. As a result, 4330 differential genes (|log₂FC| ≥ 1, FDR < 0.05) and 821 differential metabolites (|log₂FC| ≥ 1, Variable Importance in Projection, VIP > 1) were identified. Notably, most metabolites in the classes of flavonoids, phenolic acids, terpenoids, lipids and tannins were decreased under shade, except those in the amino acids and derivatives classes. KEGG enrichment analysis indicated that aminoacyl-tRNA biosynthesis was significantly upregulated while phenylpropanoid biosynthesis and gingerol biosynthesis were significantly downregulated under shade. Consistently, the contents of many amino acids were increased while several key phenylpropanoid and gingerol related metabolites were decreased under shade. Together, our study provides molecular insights into how shade cultivation may influence nutrition and flavor of Z. striolatum shoots.
姜黄嫩芽在中国被认为是一种特殊的蔬菜。遮荫栽培是提高黄曲霉嫩枝口感和品质的关键措施。然而，其潜在的机制仍然知之甚少。在本研究中，我们对光照和遮荫培养的黄曲霉芽进行了转录组学和代谢组学的联合分析。结果，鉴定出4330个差异基因（|log₂FC|≥1,FDR < 0.05）和821个差异代谢物（|log₂FC|≥1,Variable Importance in Projection, VIP > 1）。值得注意的是，除氨基酸和衍生物类外，黄酮类、酚酸类、萜类、脂类和单宁类的大多数代谢物在遮荫下减少。KEGG富集分析表明，遮荫条件下，氨酰基trna生物合成显著上调，苯丙素生物合成和姜辣素生物合成显著下调。遮荫条件下，许多氨基酸含量增加，而一些关键的苯丙素和姜辣素相关代谢物含量下降。总之，我们的研究为荫栽培如何影响黄曲霉芽的营养和风味提供了分子见解。
{"title":"Transcriptomic and metabolomic analyses reveal nutritional and flavor changes in shade-cultivated Zingiber striolatum Diels shoots","authors":"Zhengxiu Ye, Yonghong Zhang, Lanlan Zheng, Chen Li, Lei You, Xiangyin Luo","doi":"10.1016/j.scienta.2026.114711","DOIUrl":"10.1016/j.scienta.2026.114711","url":null,"abstract":"<div><div>The young shoots of <em>Zingiber striolatum</em> Diels are considered as a special vegetable in China. Shading cultivation is a key practice to improve the taste and quality of <em>Z. striolatum</em> shoot. However, the underlying mechanism remains poorly understood. In this study, we performed combined transcriptomic and metabolomic analyses of light and shade cultivated <em>Z. striolatum</em> shoot. As a result, 4330 differential genes (|log₂FC| ≥ 1, FDR < 0.05) and 821 differential metabolites (|log₂FC| ≥ 1, Variable Importance in Projection, VIP > 1) were identified. Notably, most metabolites in the classes of flavonoids, phenolic acids, terpenoids, lipids and tannins were decreased under shade, except those in the amino acids and derivatives classes. KEGG enrichment analysis indicated that aminoacyl-tRNA biosynthesis was significantly upregulated while phenylpropanoid biosynthesis and gingerol biosynthesis were significantly downregulated under shade. Consistently, the contents of many amino acids were increased while several key phenylpropanoid and gingerol related metabolites were decreased under shade. Together, our study provides molecular insights into how shade cultivation may influence nutrition and flavor of <em>Z. striolatum</em> shoots.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"358 ","pages":"Article 114711"},"PeriodicalIF":4.2,"publicationDate":"2026-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147278994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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