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Biochemical indicators to diagnose iron nutritional status of olive (Olea europaea L.) 橄榄铁营养状况的生化指标诊断
IF 4.3 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-02-04 DOI: 10.1016/j.scienta.2026.114662
Amir Hossein Khoshgoftarmanesh
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引用次数: 0
Genome-wide association study reveals candidate genes controlling root system architecture in pepper (Capsicum annuum L.) 辣椒(Capsicum annuum L.)根系结构候选基因的全基因组关联研究
IF 4.3 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-02-03 DOI: 10.1016/j.scienta.2026.114655
Han Do Bae, Joung-Ho Lee, Geon Woo Kim, Jeong Eun Kim, Jung-Min Kim, Seungki Back, Seo-Young Lee, Dae Young Kim, Jin-Kyoung Kwon, Byoung-Cheorl Kang
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引用次数: 0
Green nanoparticles-assisted dsRNA delivery enables effective control of pepper mottle virus in pepper 绿色纳米颗粒辅助dsRNA传递可有效控制辣椒斑驳病毒
IF 4.3 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-02-03 DOI: 10.1016/j.scienta.2026.114661
Min-Jae Kim, Hyo-Jeong Lee, Ju-Yeon Yoon, Jin-Sung Hong, Rae-Dong Jeong
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引用次数: 0
Exploring the potential of vermicompost in horticulture: a meta-analysis of seedling growth and sustainable cultivation 探索蚯蚓堆肥在园艺中的潜力:幼苗生长和可持续栽培的荟萃分析
IF 4.3 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-02-03 DOI: 10.1016/j.scienta.2026.114658
Fangfei Li, Yi Ren, Yixing Zhang, Jianheng Zhang, Xin-Xin Wang
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引用次数: 0
Paclobutrazol and ABA counteract GA₃-induced cork spot in ‘Akizuki’ by optimizing mineral homeostasis and cellular integrity 多效唑和ABA通过优化矿物质平衡和细胞完整性来对抗GA₃引起的“秋之草”软木斑
IF 4.3 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-02-02 DOI: 10.1016/j.scienta.2026.114659
Xiaomei Tang, Yunhui Tan, Guoling Guo, Wei Song, Kexin Cai, Luoluo Xie, Qi Liu, Tao Wu, Lun Liu, Wei Heng, Xianping Guo, Dongsheng Wang, Bing Jia
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引用次数: 0
Ethylene inhibits potato tuber sprouting during storage by regulating stress resistance and development 乙烯通过调控马铃薯块茎的抗逆性和发育来抑制马铃薯块茎的发芽
IF 4.2 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-02-01 DOI: 10.1016/j.scienta.2026.114646
Jun Wang , Hu Liu , Maorun Fu , Yuhang Chen , Yan yan , Dan Li , Qingmin Chen
Sprouting remains a serious problem during the postharvest storage of potato. The purpose of this study was to investigate the effect of exogenous ethylene on potato tuber sprouting. The potatoes were treated by exogenous ethylene solid releasers at a concentration of 1000 μL/L every 48 h. Obvious sprouts were observed in the untreated group (CK) after 2 d storage at 25 °C, while only a few potatoes began to sprout after 6 d storage under continuous treatment of ethylene (ET group). The contents of reducing and soluble sugars were higher in the ET group than in the CK. In addition, the activities of superoxide dismutase and catalase were higher in the ET group than those of the CK, while the content of malondialdehyde was reduced with ethylene treatment. Transcriptomic analysis showed that ethylene treatment increased the expression levels of stress-related genes, including those related to pathogen infection and attack, ethylene, jasmonic acid, abscisic acid, and wounding, and decreased the expression levels of genes related to auxin, gibberellin, brassinolide, the cell cycle, cell wall synthesis, and photosynthesis. Therefore, continuous ethylene treatment during storage inhibited potato sprouting by regulating stress resistance and growth. This study establishes a molecular mechanism model of the ethylene-mediated inhibition of potato sprouting, providing a theoretical basis for optimizing and developing potato bud inhibition technology during storage.
马铃薯采后贮藏中发芽是一个严重的问题。本研究旨在探讨外源乙烯对马铃薯块茎发芽的影响。用浓度为1000 μL/L的外源乙烯固体释放剂处理马铃薯,每48 h处理一次,在25℃下贮存2 d后,未处理组(CK)马铃薯出现了明显的发芽现象,而连续处理组(ET组)在贮存6 d后,只有少数马铃薯开始发芽。ET组的还原糖和可溶性糖含量高于对照。此外,ET组的超氧化物歧化酶和过氧化氢酶活性高于CK,而丙二醛含量则随乙烯处理而降低。转录组学分析表明,乙烯处理增加了胁迫相关基因的表达水平,包括与病原体感染和攻击、乙烯、茉莉酸、脱落酸和伤害相关的基因,降低了与生长素、赤霉素、油菜素内酯、细胞周期、细胞壁合成和光合作用相关的基因的表达水平。因此,贮藏期间连续乙烯处理通过调控抗逆性和生长抑制马铃薯发芽。本研究建立了乙烯介导的马铃薯发芽抑制的分子机制模型,为优化和开发马铃薯贮藏期抑芽技术提供理论依据。
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引用次数: 0
Transcriptomics-based study on the GDP-mannose synthesis pathway and salt tolerance mechanism in Auricularia heimuer 黑木耳gdp -甘露糖合成途径及耐盐机制的转录组学研究
IF 4.2 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-02-01 DOI: 10.1016/j.scienta.2026.114647
Mingbin Sun , Fangjie Yao , Lixin Lu , Xianqi Shan , Lingjian Kong , Yuan Ma
Salt stress severely limits the cultivation and productivity of Auricularia heimuer. In this study, physiological and transcriptomic analyses were integrated to elucidate the salt tolerance mechanisms of A. heimuer using a salt-tolerant strain (H2) and a salt-sensitive strain (H26). At the mycelial stage, increasing NaHCO₃ concentrations significantly inhibited growth and induced oxidative stress in both strains; however, H2 exhibited higher growth rates, lower pigment secretion, reduced membrane lipid peroxidation, and significantly enhanced antioxidant enzyme activities compared with H26. Under moderate salt stress, H2 accumulated higher levels of soluble proteins, total sugars, and trehalose, indicating superior osmotic adjustment capacity. At the fruiting body stage, salt stress altered morphology and physiological traits of H2, with moderate NaHCO₃ levels promoting osmolyte accumulation and antioxidant defense, while excessive stress caused oxidative damage and growth inhibition. Transcriptome sequencing of H2 fruiting bodies under high salt stress identified 1,342 differentially expressed genes, with significant enrichment in carbohydrate metabolism, nucleotide sugar metabolism, and protein processing in the endoplasmic reticulum. Further analysis revealed that the genes g2692 and g2546, encoding the key enzymes involved in GDP-mannose biosynthesis-mannose-6-phosphate isomerase (PMI) and GDP-mannose pyrophosphorylase (GMPP), respectively-were significantly upregulated. Quantitative expression analysis confirmed higher expression of these genes in H2 than in H26 under salt stress, accompanied by a marked increase in intracellular mannose content. These results demonstrate that enhanced osmotic regulation, antioxidant capacity, and activation of the GDP-mannose biosynthesis pathway collectively contribute to salt tolerance in A. heimuer, providing a physiological and molecular basis for improving saline-alkaline adaptability in edible fungi cultivation.
盐胁迫严重限制黑木耳的栽培和产量。本研究利用耐盐菌株H2和盐敏感菌株H26,结合生理和转录组学分析,阐明了海姆菇的耐盐机制。在菌丝期,NaHCO₃浓度的增加显著抑制了菌株的生长,诱导了氧化应激;与H26相比,H2表现出更高的生长速率、更少的色素分泌、更少的膜脂过氧化反应,并显著增强抗氧化酶活性。在中等盐胁迫下,H2积累了较高水平的可溶性蛋白、总糖和海藻糖,表明具有较强的渗透调节能力。在子实体阶段,盐胁迫改变了H2的形态和生理性状,适度的NaHCO₃水平促进渗透物积累和抗氧化防御,过量的NaHCO₃水平引起氧化损伤和生长抑制。高盐胁迫下H2子实体转录组测序鉴定出1342个差异表达基因,在内质网碳水化合物代谢、核苷酸糖代谢和蛋白质加工中显著富集。进一步分析发现,编码gdp -甘露糖生物合成关键酶甘露糖-6-磷酸异构酶(PMI)和gdp -甘露糖焦磷酸化酶(GMPP)的基因g2692和g2546显著上调。定量表达分析证实,在盐胁迫下,这些基因在H2中的表达量高于H26,同时细胞内甘露糖含量显著增加。这些结果表明,渗透调节能力增强、抗氧化能力增强以及gdp -甘露糖生物合成途径的激活共同促进了黑木耳耐盐性,为提高食用菌的盐碱适应性提供了生理和分子基础。
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引用次数: 0
Spatiotemporal effects of micro-spray misting on microclimate, physiology, and yield of apple trees 微喷雾对苹果树小气候、生理及产量的时空影响
IF 4.2 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-02-01 DOI: 10.1016/j.scienta.2026.114644
Hanmi Zhou , Cheng Chen , Linshuang Ma , Youzhen Xiang , Yidi Sun , Yumin Su , Sibo Lu , Runze Li , Zhe Peng , Jichen Li
High temperatures resulting from global climate change are severely constraining the development of the apple industry in the semi-arid region of northern China. Although micro-spray mist can improve orchard microclimate, the mechanistic response of apple growth and physiology to micro-spray mist regulation is still unclear. The experiment utilized the apple cultivar ‘Yanfu 8’, three micro-spray position levels (H1: upper canopy layer; H2: middle canopy layer; H3: lower canopy layer) and four mist duration levels (T1: 2.0 h; T2: 1.5 h; T3: 1.0 hour; T4: 0.5 h) were set up. Results indicated that while all micro-spray treatments improved the orchard microclimate, extending the misting duration from 1.5 h (T2) to 2.0 h (T1) led to tiny cooling and humidification benefits. Compared with H2T1, H2T2 exhibited superior net photosynthetic rate, leaf water use efficiency, soluble solids content, shape index and yield. The comprehensive evaluation results indicate that the RSR values were 0.950 for H2T2 and 0.831 for H2T3, ranking them as the top two treatments and classifying both as optimal grade. This means that placing the sprinkler in the middle of the canopy and using micro-spray mist for 1.5 h is an effective irrigation strategy on the growth of young trees. Under water scarcity, 1.0 h micro-spray mist is an efficient substitute. This study identifies the efficient micro-spray mist regulation measure to promote high apple yields under high-temperature conditions, providing practical guidance for orchard management.
全球气候变化导致的高温严重制约了中国北方半干旱区苹果产业的发展。微喷雾雾虽然能改善果园小气候,但苹果生长和生理对微喷雾雾调控的响应机制尚不清楚。试验以苹果品种‘盐复8号’为材料,设置3个微喷位置水平(H1:冠层上、H2:冠层中、H3:冠层下)和4个雾持续时间水平(T1: 2.0 h、T2: 1.5 h、T3: 1.0 h、T4: 0.5 h)。结果表明,虽然所有微喷处理都改善了果园小气候,但将喷雾时间从1.5 h (T2)延长至2.0 h (T1),导致降温和增湿效果很小。与H2T1相比,H2T2表现出更高的净光合速率、叶片水分利用效率、可溶性固形物含量、形状指数和产量。综合评价结果表明,H2T2的RSR值为0.950,H2T3的RSR值为0.831,将其列为前2个处理,并将其列为最优等级。这意味着,将洒水器放置在树冠的中间,并使用微喷雾雾1.5小时是幼树生长的有效灌溉策略。在缺水条件下,1.0 h的微喷雾雾是有效的替代。本研究确定了高温条件下促进苹果高产的高效微喷雾调控措施,为果园管理提供实践指导。
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引用次数: 0
Multi-omics analysis revealed the comprehensive regulatory pattern of chestnut development on pathogenic defense 多组学分析揭示了板栗发育对病原菌防御的综合调控模式
IF 4.2 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-02-01 DOI: 10.1016/j.scienta.2026.114609
Rongrong Chen , Yanyan Wu , Xinru Shi , Weiwei Sun , Guangpeng Wang , Ying Li , Shengqing Shi
Chestnut (Castanea) a globally cultivated crop, which have been threating the chestnut quality by the postharvest pathogen infections. It keeps the health status during their developmental stages, but the inner regulatory mechanism remains largely unexplored. In this study, microbiome, transcriptome, metabolomics, and defense substances were investigated in the burs, seeds, shells, and kernels collecting from developing Chinese chestnuts (C. mollissima) at 10-day intervals, spanning from the pollination to the maturation stage. Pathogen infection patterns show that during the development of chestnuts, various pathogenic fungi can colonize the developing chestnuts, with Alternaria emerging as the dominant pathogen. Notably, Filobasidium and Methylobacterium were identified as key microbial taxa throughout development. Defense substance profiling revealed a progressive decline in non-enzymatic antioxidants (polyphenol, flavonoid and tannin), contrasted with an increase in enzymatic antioxidants (SOD, POD, CAT), indicating a complementary defense strategy. The heatmap of differentially expressed genes indicates that chestnut fruit development is mainly focused on plant-pathogen interactions and genes related to chestnut growth and defense; Weighted gene co-expression network analysis highlighted critical defense genes, including CML, CPK, RPM1, and RPS3, involved in plant-pathogen interactions, as well as COMT and POD in the phenylpropanoid biosynthesis pathway. This study offers new insights into an inner defense mechanism of pathogen during chestnut development, providing a theoretical basis for enhanced chestnut cultivation and disease management.
板栗(Castanea)是一种全球性的栽培作物,其采后病原菌感染一直威胁着板栗的品质。它在发育阶段保持了健康状态,但其内部调控机制仍未完全阐明。本研究对发育中的栗子(C. mollissima)从授粉到成熟阶段每隔10天采集一次的毛囊、种子、壳和仁的微生物组学、转录组学、代谢组学和防御物质进行了研究。病原菌侵染模式表明,在栗子发育过程中,多种病原菌可在栗子发育过程中定植,其中交替菌为优势病原菌。值得注意的是,丝状菌和甲基杆菌被确定为整个发育过程中的关键微生物类群。防御物质分析显示,非酶促抗氧化剂(多酚、类黄酮和单宁)逐渐下降,而酶促抗氧化剂(SOD、POD、CAT)增加,表明一种互补的防御策略。差异表达基因热图表明,板栗果实发育主要集中在植物-病原体相互作用和板栗生长防御相关基因;加权基因共表达网络分析强调了参与植物与病原体相互作用的关键防御基因,包括CML、CPK、RPM1和RPS3,以及苯丙素生物合成途径中的COMT和POD。本研究对板栗发育过程中病原菌的内部防御机制有了新的认识,为加强板栗栽培和病害管理提供了理论依据。
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引用次数: 0
Comprehensive study of the developmental and growth processes that affect fruit yield and quality of determinate tomato under water deficit 水分亏缺条件下特定番茄发育生长过程对产量和品质影响的综合研究
IF 4.2 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-02-01 DOI: 10.1016/j.scienta.2026.114649
Leila Aslani, Thomas Breniere, Nadia Bertin
Drought is the most influential factor affecting the yield and quality of tomato crops, but the range of responses observed is considerable. In order to better understand this variability, industrial tomatoes (cv. H1311) were grown under four irrigation regimes: i) control that maintained soil water content at field capacity (Control: C), ii) 50 % reduction in water supply compared to the control from seedling to fruit harvest (deficit irrigation: DI), iii) 50 % reduction in water supply compared to the control from seedling to fruit mature green stage and then control irrigation during fruit ripening (DI-C), iv) control irrigation until fruit mature green stage and then a 50 % reduction in water supply (C-DI). Results showed that DI enhanced fresh fruit quality by increasing sugars, acids, polyphenols, and carotenoid contents, but most of these effects were attributable to higher dry matter content, as the dry matter concentration of these compounds was not affected or even reduced. Only naringenin, rutin and total polyphenol contents exhibited additive concentration and metabolic effects under DI, as their concentrations increased on both the fresh and dry mass bases. Both C-DI and DI-C treatments had less effect on fruit quality traits than DI. At the plant level, DI strongly reduced vegetative and reproductive development in similar proportions, as well as leaf area and photosynthetic capacity through both stomatal and non-stomatal factors. As a consequence, the commercial fruit yield was strongly reduced under DI, due to decreases in fruit number and fruit growth rate. Altogether, the results suggested that yield reduction resulted from both source and sink limitations under DI. Overall, because commercial fresh and dry yields per plant were similar in C and DI treatment, water removal during the ripening period (C-DI) remained the most competitive strategy in terms of WUE, whereas constant DI might be the most favorable for fresh fruit quality.
干旱是影响番茄产量和品质的最主要因素,但干旱对番茄产量和品质的影响范围相当大。为了更好地理解这种可变性,工业番茄(cv。H1311)在四种灌溉制度下生长:i)控制土壤含水量保持在田间容量(对照:C), ii)从苗期到果实收获的供水比对照减少50%(亏缺灌溉;DI), iii)与对照相比,从苗期到果实成熟绿期供水减少50%,然后在果实成熟期间控制灌溉(DI- c), iv)控制灌溉直到果实成熟绿期,然后供水减少50% (C-DI)。结果表明,DI通过增加糖、酸、多酚和类胡萝卜素的含量来提高鲜果品质,但这些作用主要是由于干物质含量的增加,这些化合物的干物质浓度没有受到影响,甚至没有降低。只有柚皮素、芦丁和总多酚含量在DI处理下表现出添加浓度和代谢效应,其在鲜、干质量基础上的浓度均呈增加趋势。C-DI和DI- c处理对果实品质性状的影响均小于DI处理。在植物水平上,DI通过气孔和非气孔因素,以相似的比例显著降低营养发育和生殖发育,以及叶面积和光合能力。结果,由于果实数量和果实生长速度的下降,DI处理下商品果实产量大幅下降。综上所述,在DI条件下,产量降低既有源限制,也有汇限制。总体而言,由于C和DI处理的单株商业鲜干产量相似,因此在WUE方面,成熟期除水(C-DI)仍然是最具竞争力的策略,而恒定的DI可能对新鲜水果品质最有利。
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引用次数: 0
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Scientia Horticulturae
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