Scientia Horticulturae最新文献

{"title":"Altering growth and antioxidant responses of basil cultivars with aluminum oxide nanoparticles in vitro","authors":"Abeer A. Radi,&nbsp;Fatma A. Farghaly,&nbsp;Aya H. Abd-El-Mageed,&nbsp;Afaf M. Hamada","doi":"10.1016/j.scienta.2024.113857","DOIUrl":"10.1016/j.scienta.2024.113857","url":null,"abstract":"<div><div>The rapid advancement of nanotechnology has resulted in a significant increase in the production of metal oxide nanoparticles, which are increasingly released into the environment. Due to their widespread distribution, evaluating the potential toxicity of these particles is essential. Aluminum oxide nanoparticles (Al₂O₃ NPs), in particular, are widely used in various industries and consumer products. Basil, a valuable medicinal herb known for its essential oils and antioxidants, has numerous health benefits. The impact of Al₂O₃ NPs on plants remains largely unexplored. This study investigated the effect of different concentrations of Al₂O₃ NPs and their bulk form (AlCl₃; BP) on the growth of red Rubin and sweet basil cultivars <em>in vitro</em>, focusing on the induction of non-enzymatic and enzymatic antioxidant responses. Growth parameters were adversely affected by variations in cultivar, Al particle size, and concentration. The highest reductions occurred at 200 mg L⁻¹ NPs or BPs, with decreases of up to 69.34 % for red Rubin and 63.33 % for sweet basil. Al NPs and BPs reduced chlorophyll <em>a, b,</em> and carotenoid levels. These pigments showed the highest decreases at 200 mg L⁻¹, with reductions of up to 81.92 % for red Rubin and 75.96 % for sweet basil. Al NPs and BPs compromised membrane integrity, inducing oxidative stress, as evidenced by increased electrolyte leakage, UV-absorbing compounds, malondialdehyde (MDA), and hydrogen peroxide (H₂O₂) levels. The phenolic and amino acid concentrations in red Rubin basil leaves and roots decreased in response to increasing NP concentrations, while the opposite trend was observed for BPs. Conversely, sweet basil showed a consistent upward trend in phenolic and amino acid levels as Al concentrations increased, irrespective of particle form. The content of soluble proteins in basil leaves and roots declined as the concentrations of NPs and BPs increased. Enzyme activities, including superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), phenylalanine ammonia-lyase (PAL), and polyphenol oxidase (PPO), increased in basil leaves and roots when exposed to rising concentrations of NPs and BPs. Catalase (CAT) activity increased in red Rubin leaves and roots but decreased in sweet basil leaves and roots, with rising concentrations of NPs and BPs. The observed variations in leaf and root growth between the two basil cultivars exposed to different concentrations of Al NPs and BPs suggest that cultivar physiology and particle characteristics play a role. Additional studies are required to clarify these mechanisms.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"339 ","pages":"Article 113857"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Grapevine and cover crop spectral response to evaluate vineyard spatio-temporal variability","authors":"Pietro Catania,&nbsp;Massimo Vincenzo Ferro,&nbsp;Santo Orlando,&nbsp;Mariangela Vallone","doi":"10.1016/j.scienta.2024.113844","DOIUrl":"10.1016/j.scienta.2024.113844","url":null,"abstract":"<div><div>The use of cover crops in vineyards is considered a management strategy that contributes to improving soil structure. Remote sensing techniques of cover crops provide information on spatial variability that can be useful in precision viticulture. This study aims to investigate soil-plant interactions using biometric data, spectral response and soil physico-chemical parameters measured across 2021–2022, with the scope of assessing spatio-temporal variations of cover crop and vineyard. These evaluations were conducted on an on-farm vineyard in a semi-arid Mediterranean environment. During the winter season (T1), the cover crop (<em>Vicia Faba</em>) growth was evaluated. Equally, during the summer season (T2), vineyard monitoring was carried out during the phenological stages of flowering (T2A) and grape ripening (T2B). Multispectral images acquired through unmanned aerial vehicle (UAV) were employed, and subsequently, the normalized difference vegetation index (NDVI) was calculated to obtain crops vigor maps. Through bivariate LISA cluster maps, cover crop vigor maps were compared with those of the vineyard, revealing the presence of significant positive spatial autocorrelation between the two crops. Indeed, in areas where the cover crop exhibited higher dry matter accumulation, grapevine high vigor growth was observed. The spatial association identified in surveys conducted in T1 and T2 was examined considering soil parameters. It emerged that in areas where both the cover crop and the vines exhibited high NDVI values, soil factors such as soil organic carbon (SOC) content and total nitrogen (TN) were higher. Conversely, in areas where both crops showed low NDVI values, they were associated with higher soil bulk density (BD). Moreover, in low vigor zones, higher soil penetration resistance (SPR) values were observed in both T1 and T2, contributing to limiting root elongation and thus reducing overall crop growth. Vegetative growth variability observed through vigor maps and soil physicochemical properties was evaluated through principal component analysis (PCA). Soil parameters considered within these components highlighted their influence on crop growth. This study emphasizes how soil factors directly influence the spatiotemporal variability of crops. Monitoring cover crops allows for early detection of spatial variability, supporting vine growers in choosing sustainable vineyard management techniques.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"339 ","pages":"Article 113844"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensively assessing the effects of exogenous proline on nutritional and flavour quality of celery (Apium graveolens L.) under salt stress","authors":"Yanqiang Gao,&nbsp;Jing Zhang,&nbsp;Cheng Wang,&nbsp;Nenghui Li,&nbsp;Kaiguo Pu,&nbsp;Tiantian Wang,&nbsp;Miao Zhang,&nbsp;Liangmei Zhang,&nbsp;Jing Li,&nbsp;Jianming Xie","doi":"10.1016/j.scienta.2024.113847","DOIUrl":"10.1016/j.scienta.2024.113847","url":null,"abstract":"<div><div>Salt stress is one of the constraints limiting high-yield and high-quality cultivation of vegetables, currently, the effect of exogenous proline on the quality of celery under salt stress is rarely reported. The study aimed to explore the comprehensive effects of exogenous proline application on the quality of celery (<em>Apium graveolens</em> L.) under salt stress. The affiliation function method was used to comprehensively analyze the effects of foliar application of 0.3 mM proline on celery mineral nutrients, amino acids, organic acids, nitrate, dietary fiber, and volatile components under 100 mM NaCl stress. Results showed that exogenous proline application inhibited Na content and nitrate enrichment in celery, and promoted the accumulation of other mineral elements (N, P, K, Ca, Mg, Cu, Fe, Mn and Zn), glycine, oxalic acid, malic acid, citric acid, lignin, dietary fiber, and aromatic compounds in leaves and petioles under salt stress. In addition, the results of a comprehensive evaluation analysis of the affiliation function indicated that the application of exogenous proline to salt-stressed seedlings was an effective treatment which significantly mitigated the salt-induced detrimental effects. Overall, exogenous proline played a positive role in regulating the balance of mineral elements, accumulation of glycine, accumulation of organic acids, and enrichment of aromatic substances in celery under salt stress. Results from this study provides a theoretical basis for cultivating cultivation of high quality celery under saline conditions.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"339 ","pages":"Article 113847"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The synergy of ACC deaminase producing plant growth-promoting microbes provide drought tolerance in Ocimum basilicum L. cv. “Saumya”","authors":"Suman Singh ,&nbsp;Shiv Shanker Pandey ,&nbsp;Chandan Singh Chanotiya ,&nbsp;Deepti Barnawal ,&nbsp;Poornima Vajpayee ,&nbsp;Alok Kalra","doi":"10.1016/j.scienta.2024.113810","DOIUrl":"10.1016/j.scienta.2024.113810","url":null,"abstract":"<div><div>The use of plant growth-promoting microorganisms is an effective agricultural practice to improve plant growth, especially under abiotic stress. In this study, the combined impact of three plant growth-promoting bacteria (PGPB) namely <em>Brevibacterium halotolerans</em> (Sd-6), <em>Burkholderia cepacia</em> (Art-7), <em>Bacillus subtilis</em> (Ldr-2) were tested with <em>Trichoderma harzianum</em> (Th) (possessing ACC deaminase producing activity) in <em>Ocimum basilicum</em> L. cv. “Saumya” to reduce drought-induced damages to the plants under different level of drought stress [i.e. well-watered (100 %), moderate (60 %), severe (40 %)]. These PGPB strains, along with Th, were found to be tolerant against osmotic stress when tested in growth media containing different concentrations of polyethylene glycol (PEG 8000), and all were found to endure -0.99 MPa water potential. Compared to non-inoculated control, Th+Ldr-2 treatment improved fresh herb weight (62.45 %) and oil content (61.54 %) and higher photosynthetic rate under severe drought. Besides, in relation to control, the above treatment enhanced nutrient uptake, reduced ABA, ACC as well as ethylene levels and increased IAA content in addition to an increase in important constituents of essential oil, indicating better performance in terms of plant growth under drought. Higher RWC, decreased MDA, and reduced antioxidant activities in Th+Ldr-2 treated plants compared to non-inoculated control under drought support the mechanism of the microbes providing tolerance against drought. Colony forming unit of microbes and scanning electron microscopy (SEM) study support the effective colonisation behaviour of Th+Ldr-2, which protects plants against drought stress. A consortium of diverse microbes, found to improve plant growth under drought through increased nutrient uptake, reducing the levels of ACC and ABA, improving the content of IAA, antioxidant enzymes probably reducing the effect of drought stress and improving plant biomass could be a useful tool to reduce drought-induced losses in crop plants.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"339 ","pages":"Article 113810"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The timing and pattern of chill and heat exposure affect the estimation of agroclimatic requirements and adaptability potential in Prunus cultivars","authors":"Alvaro Delgado ,&nbsp;Jose A. Egea ,&nbsp;Jesús López-Alcolea ,&nbsp;José Antonio Campoy ,&nbsp;David Ruiz","doi":"10.1016/j.scienta.2024.113910","DOIUrl":"10.1016/j.scienta.2024.113910","url":null,"abstract":"<div><div>A series of anomalies can occur in temperate fruit trees cultivated in mild winter regions where the fulfilment of chill requirements can be compromised in some seasons. Some symptoms include erratic bud break, bud abortion and ultimately, low fruit set. In this work, we experimentally determined the chill and heat requirements of 14 <em>Prunus</em> spp. cultivars, including apricot, almond and peach, across two locations with varying levels of chill and heat accumulation (cold/mild versus warm climatic conditions) during two successive growing seasons in Murcia (South-Eastern Spain). Chill accumulation varied considerably among the studied sites and seasons. In general, for the same cultivar, chill requirements (CR) were lower and heat requirements (HR) were higher in warm locations compared to cold/mild locations. Moreover, the results indicated that year-to-year fluctuations in both total amount of chill and chill exposure patterns had an impact on the quantification of CR and HR, particularly in warm areas. To understand the effect of the amount and dynamics of chill accumulation in the adaptability of the same cultivar in contrasting environments, we evaluated two climatic adaptation variables: the percentage of flower buds reaching flowers and the percentage of fruit set. Overall, the correlation between the initial number of flower buds and fruit set was notably stronger during the season with higher chill accumulation, especially in warm locations. The most obvious symptoms of poor adaptability caused by the lack of chill were observed during the season on which the accumulation of chill during November and December was particularly scarce. This information suggests that in chill-limited situations, the timing and pattern of chill accumulation rather than the total amount of chill during the season affects dormancy release, flowering and productivity. Finally, the results appear to show a distinct phenotypic plasticity in response to chill exposure among cultivars.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"339 ","pages":"Article 113910"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel approach for enhancing sprouting to increase yield and its mechanism in Fraxinus mandshurica Rupr.","authors":"Jianfei Liu,&nbsp;Kezhuo Fu,&nbsp;Boyao Li,&nbsp;Qiaoxin Wang,&nbsp;Yang Cao,&nbsp;Yaguang Zhan","doi":"10.1016/j.scienta.2024.113897","DOIUrl":"10.1016/j.scienta.2024.113897","url":null,"abstract":"<div><div><em>Fraxinus mandshurica</em> is a valuable timber and landscaping tree species in northern China. However, its clonal propagation faces significant challenges, including limited sprouting of young mother trees, insufficient scion sources, low yields and short usage cycles. This study introduced a novel method to promote sprouting in <em>F. mandshurica</em>, focusing on factors like trunk cutting, continuous rejuvenation and leaf trimming. The method initially determined the optimal conditions for trunk cutting, considering tree age, height, and timing. Plants that met these conditions were then subjected to coppicing, basal girdling and hormone sprays rejuvenation treatments in sequence. Leaf trimming was conducted during sprouting branches collection. The results showed that for trees over 4 years old, trunk cutting treatment was performed 30∼50 cm above the ground 3 weeks after sap flow in spring, followed by rejuvenation treatment (coppicing + basal girdling + hormone sprays), which improved the cutting yield by 10.4-fold. Furthermore, retaining a pair of axillary buds at the base while removing the compound leaves during branch harvesting can increase cutting yield by an additional 47.1 %. This approach effectively promoted sprouting and produced cuttings suitable for propagation. To further elucidate the mechanism of sprouting promotion, the hormone levels and key gene expression during shoot regeneration after <em>F. mandshurica</em> trunk cutting were analyzed, leading to the identification of the key shoot regeneration gene <em>FmESR1</em>. By transforming the <em>F. mandshurica</em> hypocotyl with <em>FmESR1</em>, overexpressing transgenic plants were obtained. Combined with the changes in adventitious shoot development, hormone levels and regeneration-related gene expression, it was revealed that <em>FmESR1</em> played a crucial role in shoot regeneration. Thus, it was concluded that sprouting promotion significantly improved regeneration ability and cutting yield by activating the expression of <em>FmESR1</em>. This technology lays a solid foundation for the efficient reproduction and widespread application of superior and new varieties of <em>F. mandshurica</em>. The elucidation of <em>FmESR1</em> function not only enriches the theoretical understanding of shoot regeneration and the gene function database but also provides theoretical and technical support for asexual propagation.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"339 ","pages":"Article 113897"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variation of Camellia oleifera fruit traits and nutritional constituents in seed oil during development and post-harvest","authors":"Qingbo Kong ,&nbsp;Tao Chen ,&nbsp;Heng Wang ,&nbsp;Shiheng Zheng ,&nbsp;Haizhou Wang ,&nbsp;Heng Liang ,&nbsp;Lijun Zhou ,&nbsp;Hongyu Yang ,&nbsp;Xiaoyu Jiang ,&nbsp;Chunbang Ding ,&nbsp;Shiling Feng","doi":"10.1016/j.scienta.2024.113903","DOIUrl":"10.1016/j.scienta.2024.113903","url":null,"abstract":"<div><div><em>Camellia oleifera</em> Abel<em>.</em> is a unique and important edible oil crop in China. The quality and high yield of <em>C. oleifera</em> are closely related to cultivation, management and back-end processing and storage. However, studies on the growth and development, drying treatment and back-end storage of <em>C. oleifera</em> are not comprehensive enough. Therefore, the phenotypic characteristics of <em>C.oleifera</em> fruits during growth and ripening were studied and the effects of four different drying methods including room temperature drying (SW), hot air drying (RF, 65 °C), vacuum drying (ZG, 50 °C, 0.6 Mpa) and freeze drying (LD) on the oil content of <em>C.oleifera</em> seeds (COS) were discussed. At the same time, the changes of the main components and nutrients of <em>C.oleifera</em> kernel oil (COKO) under room temperature storage (SG) and 4 °C frozen storage (LC) were also investigated. The results of the study showed that <em>C. oleifera</em> cultivar ‘Changlin40’ exhibited an S-shaped curve in fruit growth and development. In addition, the oil content initially increased and then remained stable, and the soluble protein and starch contents increased with increasing fruit maturity, but the soluble sugar content increased rapidly at the early stage of fruit development and gradually decreased at the later stage. The maximum content of squalene in the oil obtained by vacuum drying was 80.578 μg/g. The oil content of COS treated by RF was 55.31%, significantly higher than the other treatments. In addition, it was also found that with increasing duration of SG, the acid and peroxide values of COKO increased significantly and the nutrients such as tocopherol, squalene and sterol decreased significantly, which was exactly the opposite of LC, indicating that LC could reduce the loss of nutrients during storage. This study can provide a theoretical basis for field management, screening of drying methods and subsequent storage of <em>C. oleifera</em>.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"339 ","pages":"Article 113903"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142889399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preharvest methyl jasmonate application regulates ripening, colour development and improves phytochemical quality of fruits: A review","authors":"Mahmood Ul Hasan,&nbsp;Zora Singh,&nbsp;Hafiz Muhammad Shoaib Shah,&nbsp;Jashanpreet Kaur,&nbsp;Andrew Woodward,&nbsp;Eben Afrifa-Yamoah,&nbsp;Mekhala Dinushi Kananke Vithana","doi":"10.1016/j.scienta.2024.113909","DOIUrl":"10.1016/j.scienta.2024.113909","url":null,"abstract":"<div><div>Jasmonic acid (JA) and its methyl ester, methyl jasmonate (MeJA), are increasingly being recognised as unique phytohormones, linked to a variety of physiological and molecular functions. Endogenous concentrations of jasmonates (JAs) vary among plant parts, stages of fruit development, maturity, ripening and during the postharvest period. MeJA expresses prime cellular responses, where as an elicitor of secondary metabolite production, aids in inter-plant communications, regulates the biosynthesis of associated phytohormones, supports plant defence systems against pathogenic infections, and helps in abiotic stress conditions. The potential of MeJA has been extensively studied in modulating fruit ripening, enhancing colour development, and improving the phytochemical profile, particularly for antioxidants in fruit crops. Preharvest application of MeJA regulates ethylene biosynthesis during fruit maturation and ripening. Preharvest MeJA sprays have been shown to significantly increase the biosynthesis of phytochemicals such as phenolics, flavonoids, anthocyanins, carotenoids, enzymatic and non-enzymatic antioxidants, as well as essential nutrients. Preharvest MeJA application also upregulates the production of aroma volatiles in fruits. Preharvest spray of MeJA is reported to alleviate chilling injury in cold-stored fruits. This review explores: the biosynthesis of JAs, their influence on tree growth; fruit ripening physiology; colour development; regulation of biosynthesis of pigments; fruit firmness and modulation of biochemical attributes, including antioxidant compounds, during the ripening process, at harvest, and during postharvest periods; therby bridging gaps towards a more comprehensive understanding.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"339 ","pages":"Article 113909"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative metabolomic analysis reveals key metabolites associated with blackheart development in pineapple","authors":"Yuting Tu ,&nbsp;Yanggui Xu ,&nbsp;Zhiping Peng ,&nbsp;Yiping Peng ,&nbsp;Sai Xu ,&nbsp;Zhuxian Li ,&nbsp;Jianyi Liang ,&nbsp;Wenliang Zhong ,&nbsp;Jichuan Huang","doi":"10.1016/j.scienta.2024.113902","DOIUrl":"10.1016/j.scienta.2024.113902","url":null,"abstract":"<div><div>Pineapple, a vital tropical fruit worldwide, faces economic losses due to blackheart disorder, a complex physiological disorder influenced by various factors, including abrupt temperature changes. This study employed Ultra-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry (UPLC-ESI-MS/MS) to investigate the metabolic alterations in pineapple fruits <em>Ananas comosus</em> L. Merr. cv. 'Comte de Paris' associated with different stages of blackheart disorder. A total of 268 metabolites were detected in pineapple pulp at varying blackheart disorder stages according to the metabolomic profiling. Twelve differentially accumulated metabolites (DAMs) (Cyclic AMP, Senecionine, 3′-AMP, Cyclic GMP, 3′-GMP, beta-Alanyl-<em>L</em>-arginine, gamma-Glutamylalanine, beta-Alanyl-<em>L</em>-lysine, GMP, R-b-aminoisobutyric acid, <em>L</em>-Homophenylalanine, <em>L</em>-Arginine) were significantly enriched in healthy pineapple fruit tissues, with their levels negatively correlated with the severity of internal blackheart disorder in pineapples. Additionally, four potential biomarkers such as pipecolic acid, chlorogenic acid, phenylethylamine, and stachyose were identified, due to their significantly enriched in the internal blackheart disorder pineapple tissues, with their levels positively correlated with the blackheart severity rate. This study provides valuable insights into the metabolic pathways involved in pineapple blackheart disorder. The identified metabolites offer potential biomarkers for understanding physiological disorder progression, laying the groundwork for future studies focused on the prevention and control of pineapple blackheart disorder, ultimately safeguarding the economic value of this tropical fruit.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"339 ","pages":"Article 113902"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptome analysis of leaf color in 'Shine Muscat' and 'ZhongShan-HongYu' hybrids","authors":"Haoran Li,&nbsp;Yi Zhang,&nbsp;Hui Li,&nbsp;Yaxin Yang,&nbsp;Yang Dong,&nbsp;Chenxu Sun,&nbsp;Huan Zheng,&nbsp;Jianmin Tao","doi":"10.1016/j.scienta.2024.113896","DOIUrl":"10.1016/j.scienta.2024.113896","url":null,"abstract":"<div><div>The colour of plant leaves undergoes changes during different growth stages, with anthocyanins playing a pivotal role in this process. 'Shine Muscat' (MS) is a white-flesh green-leaf variety, while 'ZhongShan-HongYu' (ZS-HY) is a red-flesh red-leaf variety. The leaves of their hybrid offspring show different colour in autumn. However, the anthocyanin biosynthetic pathway in these varieties remains poorly understood. In this study, we present the results of the transcriptomic analysis of the hybrid progeny of 'MS' and 'ZS-HY' indicated that the red-fleshed progeny (R-type) exhibited a significantly enhanced capacity for anthocyanin accumulation in their leaves compared to the white-fleshed progeny (L-type). The transcriptomic analysis identified 639 and 3885 differentially expressed genes (DEGs) when comparing GGR (red leaf stage G) vs GGL (green leaf stage G) and GGR (red leaf stage G) vs GCR (red leaf stage C), respectively. The KEGG analysis revealed that the DEGs were predominantly enriched in the metabolism of tryptophan and the biosynthesis of flavonoids. Furthermore, the analysis identified a number of key genes involved in anthocyanin synthesis, including <em>LDOX, CHS, FLS1, CHS3, LAR1, F3H, ANR, DFR, F3′5′H, UFGT, LAR2, CHI, F3′H,</em> and so forth. WGCNA revealed that the cyan module correlates with the transition in leaf colour in red-leaf plants, thus unravelling the complex network of genes and transcription factors involved in anthocyanin biosynthesis. Transcriptomic analysis demonstrated that the significant differential expression of <em>VvHY5, VvWRKY44</em> and <em>VvMYBA6</em> is crucial for the synthesis of R-type anthocyanins.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"339 ","pages":"Article 113896"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}