Scientia Horticulturae最新文献_第4页

Identification and biological characteristics of Colletotrichum species combined with cultivar response analysis unravel grape ripe rot resistance mechanisms 炭疽病菌的鉴定、生物学特性及品种反应分析揭示了葡萄抗成熟腐病的机理

IF 4.3 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae

Pub Date : 2026-01-31 DOI: 10.1016/j.scienta.2026.114653

Shiping Mei, Runlong Zheng, Wenteng Sun, Tingting Kang, Xiaofeng Shang, Yu Hao, Yan Xu, Wen Zhang, Guotian Liu

引用次数: 0

Corrigendum to “Aluminum stress alters leaf physiology and endophytic bacterial communities in ginseng (Panax ginseng Meyer)” [Scientia Horticulturae, Volume 350, August 2025, 114276] “铝胁迫改变人参（Panax ginseng Meyer）的叶片生理和内生细菌群落”的勘误[科学园艺，卷350，August 2025, 114276]

IF 4.3 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae

Pub Date : 2026-01-25 DOI: 10.1016/j.scienta.2026.114636

Chijia Liang, Fan Zhou, Guanzhong Ding, Peng Mu, Yue Zhang, Ning Liu

引用次数: 0

Gallic acid as a phytostimulant enhances the yield and fruit nutrient profile of greenhouse-grown cucumber under mulching, deficit- and well-watered conditions 没食子酸作为一种植物兴奋剂，在覆盖、亏缺和水分充足的条件下提高了温室黄瓜的产量和果实营养成分

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae

Pub Date : 2026-01-24 DOI: 10.1016/j.scienta.2025.114479

Fahad Kimera , Muziri Mugwanya , Hani Sewilam

Water stress is a major factor hindering horticulture production in marginal areas. Applying phytostimulants combined with deficit drip irrigation and mulching could present benefits to improve the yield and quality of cultivated crops. This study investigated the impact of foliar application of different concentrations of gallic acid (T1: control [deionized water], T2: 100 µM, T3: 200 µM, T4: 300 µM, T5: 400 µM, and T6: 500 µM) to mulched and non-mulched cucumber crops under deficit (50 % field capacity [FC]) and well-watered (100 % FC) conditions. The study results indicated that severe water stress (50 % FC) negatively impacted the plant heights, leaf area, leaf area index, chlorophyll content (SPAD), and yield of cucumber, especially under the non-mulched treatments. However, mulching significantly improved the fruit yield by 74.59 %, 64.63 %, and 60.88 % in T6, T5, and T4, respectively, compared to T1 at 50 % FC. Nonetheless, deficit drip irrigation improved the water productivity by 54.66 % and 48.88 % in mulched and non-mulched treatments, respectively. For fruit nutrient composition, gallic acid treatments improved the nutrient composition of fruits in deficit and well-watered conditions, irrespective of mulching or non-mulching conditions. Based on these results, this study recommends foliar application of gallic acid at a concentration of 300 µM under mulched and deficit irrigation (50 % FC) conditions due to its superior effect on yield, water productivity, and fruit nutrient composition.

水分胁迫是阻碍边缘地区园艺生产的主要因素。施用植物兴奋剂与亏缺滴灌和覆盖相结合，有利于提高栽培作物的产量和品质。本研究研究了不同浓度的没食子酸（T1：对照[去离子水]、T2: 100µM、T3: 200µM、T4: 300µM、T5: 400µM和T6: 500µM）在亏缺（50%田容量[FC]）和水分充足（100% FC）条件下对覆盖和未覆盖黄瓜作物的影响。研究结果表明，重度水分胁迫（50% FC）对黄瓜株高、叶面积、叶面积指数、叶绿素含量（SPAD）和产量均有显著的负向影响，特别是在不覆盖处理下。但在50% FC条件下，与T1相比，覆盖在T6、T5和T4的产量分别显著提高了74.59%、64.63%和60.88%。亏缺滴灌在覆盖和不覆盖处理下，水分生产力分别提高了54.66%和48.88%。在果实营养成分方面，无论覆盖或不覆盖，没食子酸处理均改善了亏缺和丰水条件下果实的营养成分。基于这些结果，本研究建议在覆盖和亏缺灌溉（50% FC）条件下叶面施用浓度为300µM的没食子酸，因为它对产量、水分生产力和果实营养成分有更好的影响。

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引用次数: 0

Drought tolerance assessment of carrot genotypes: A morphological and physiological approach 胡萝卜基因型的耐旱性评价：形态学和生理学方法

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae

Pub Date : 2026-01-24 DOI: 10.1016/j.scienta.2026.114637

Md. Faruk Hossain , Md. Harun Ar Rashid , Md. Abdur Rahim , Md. Golam Rabbani

Drought tolerance is a critical focus in crop improvement, particularly in carrots (Daucus carota L.), a globally significant vegetable. One approach to addressing this issue is to develop varieties that are tolerant to drought stress. The research was conducted in two locations in Bangladesh, farmer fields of the Teesta River lands, Kaunia region of the Rangpur district and the Horticulture Farm, Bangladesh Agricultural University, Mymensingh-2202 from November 2022 to February 2023, with varying soil and climatic conditions. The experiment was laid out in a Split Plot Design with two replications. This study aims to assess the drought tolerance of carrot genotypes, incorporating both morphological and physiological traits to evaluate their performance under drought stress. Drought stress was imposed during the critical taproot development stage. Results indicated significant genotype-dependent variability in response to drought. Genotypes G22 (PI 419042), G24 (PI 419184) and G16 (PI 264669) consistently exhibited superior drought tolerance with high marketable root yield, maintaining higher relative water content, proline accumulation and chlorophyll content, contributing to their resilience. Root traits, such as root length and diameter, and root fresh weight were positively correlated with yield under both control and drought conditions. According to the wilting severity scale G22 (PI 419042), G24 (PI 419184) and G16 (PI 264669) were the most drought-stress tolerant. The biplot analysis, based on the Multi-trait genotype-ideotype distance index (MGIDI), highlighted genotypes G22 (PI 419042), G24 (PI 419184) and G16 (PI 264669) as the top performers due to their desirable trait combinations. This research provides valuable insights into the genetic variability of drought tolerance in carrots, aiding the development of resilient cultivars for drought-prone environments.

耐旱性是作物改良的关键焦点，特别是胡萝卜（Daucus carota L.），一种全球重要的蔬菜。解决这一问题的一种方法是开发耐干旱胁迫的品种。该研究于2022年11月至2023年2月在孟加拉国的两个地点进行，分别是Rangpur地区Kaunia地区Teesta河土地的农民田地和孟加拉国农业大学Mymensingh-2202的园艺农场，土壤和气候条件各不相同。试验采用裂区设计，两个重复。本研究旨在从形态和生理两方面评价胡萝卜基因型在干旱胁迫下的抗旱性。干旱胁迫发生在主根发育关键期。结果表明，在对干旱的响应中存在显著的基因型依赖性变异。基因型G22 （PI 419042）、G24 （PI 419184）和G16 （PI 264669）均表现出较强的抗旱性，具有较高的可销根产量，保持较高的相对含水量、脯氨酸积累和叶绿素含量，有利于其抗旱性。在对照和干旱条件下，根长、根径、根鲜重等根系性状与产量均呈正相关。根据萎蔫程度评分，G22 （PI 419042）、G24 （PI 419184）和G16 （PI 264669）的抗旱能力最强。基于多性状基因型-理想型距离指数（MGIDI）的双图分析显示，基因型G22 （PI 419042）、G24 （PI 419184）和G16 （PI 264669）因其理想的性状组合而表现最佳。这项研究为胡萝卜耐旱性的遗传变异提供了有价值的见解，有助于在干旱易发环境中开发具有抗旱性的品种。

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引用次数: 0

Geometric and radiometric analysis of dual wavelength NIR-LiDAR scanning for estimating physiological and free water of leaves in apple tree canopies 双波长NIR-LiDAR扫描估算苹果树冠层叶片生理水分和游离水分的几何和辐射分析

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae

Pub Date : 2026-01-24 DOI: 10.1016/j.scienta.2025.114564

Nicolas Tapia-Zapata, Manuela Zude-Sasse

Current challenges in the production of fruit and vegetables are increasing with climate change, impacting water requirements in different production operations. Although water status of plants and surface wetness detection have been widely investigated using diverse sensors and well established methodologies, spatial resolution and the request to monitor potentially all plants are continuous trade-off in large orchard systems. Thereof, light detection and ranging systems (LiDAR) are gaining rapid interest in horticultural research due to its ability to provide geometric and radiometric data of plants in field conditions by means of three-dimensional point clouds. Previous experiments have been approaching close-range sensing of leaf water content (LWC) based on the absorption of electromagnetic wave by water in the near infrared wavelength range. This paper is aimed at assessing the water status of apple canopies using a dual-wavelength LiDAR scanner at 1320 and 1450 nm. Physiological water was measured by LWC, leaf water potential, and crop water stress index. Additionally, the sensor responses to spray application of water on the canopies was investigated. A semi-automated processing algorithm was developed to isolate points of leaves from wood structures. Normalized returned signal strength intensity (

I_{N o r m}

) was obtained at 1320 and 1450 nm after radiometric calibration. A normalized difference NIR index (

N D N I R

) is proposed, based on the two wavelengths. Only marginal differences were found between

I_{N o r m}

values, comparing fully irrigated and drought stressed canopies for all LiDAR derived variables (

I^{1320}

,

I^{1450}

and

N D N I R

). Only LWC was slightly correlated to

I^{1450}

, with adjusted

R^{2}

of 0.3. On the other hand, significant differences were found between dry and sprayed canopies, with free water on the leaves, comparing patterns at

I^{1450}

and

N D N I R

at 95 % confidence interval using Kolmogorov-Smirnov statistical test. Benefits and challenges of the methodology point to feasible applications in detecting free water on the leaf surface under field conditions.

随着气候变化，水果和蔬菜生产面临的挑战越来越多，影响了不同生产操作中的用水需求。尽管植物的水分状况和表面湿度检测已经使用各种传感器和完善的方法进行了广泛的研究，但在大型果园系统中，空间分辨率和监测潜在所有植物的要求是持续的权衡。因此，光探测和测距系统（LiDAR）在园艺研究中获得了迅速的兴趣，因为它能够通过三维点云在野外条件下提供植物的几何和辐射数据。以往的实验都是基于水对近红外波长范围内电磁波的吸收，来接近近距离地感知叶片含水量（LWC）。本文旨在利用1320和1450 nm双波长激光雷达扫描仪评估苹果冠层的水分状况。采用LWC、叶片水势和作物水分胁迫指数测定生理水分。此外，还研究了传感器对冠层喷水的响应。开发了一种半自动处理算法，将树叶点与木结构分离开来。在1320和1450 nm处得到归一化返回信号强度（INorm）。提出了一种基于两个波长的归一化差分近红外指数（NDNIR）。通过比较所有LiDAR衍生变量（I1320， I1450和NDNIR）的完全灌溉和干旱胁迫冠层，INorm值之间只有微小差异。只有LWC与I1450略有相关，调整后R2为0.3。另一方面，使用Kolmogorov-Smirnov统计检验，在I1450和NDNIR（95% %置信区间）下，干燥冠层与喷洒冠层之间存在显著差异。该方法的优点和挑战指出了在野外条件下检测叶片表面自由水的可行应用。

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引用次数: 0

Improving the shelf life and quality of edible white mushrooms with modified atmosphere packaging technique: experimental evaluation and mathematical simulation of moisture reduction 改良气调包装技术提高食用白蘑菇的保质期和品质：减湿的实验评价和数学模拟

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae

Pub Date : 2026-01-24 DOI: 10.1016/j.scienta.2026.114617

Sh. Asadbeigi, E. Ahmadi

This study investigates moisture dynamics in white button mushrooms (Agaricus bisporus) during storage under modified and non-modified atmosphere packaging at two temperatures (4 °C and 25 °C) using combined experimental measurements and numerical modeling. Experimental results showed an average moisture reduction of approximately 11% over a 10-day storage period, with significantly higher losses observed under ambient temperature and non-modified atmosphere conditions. A Multiphysics model developed in COMSOL, coupling laminar airflow, heat transfer in moist air, and moisture transport in porous media, was validated using experimental moisture data and predicted a comparable moisture reduction of about 16%, capturing the observed exponential decay trend. The simulation further demonstrated that modified atmosphere packaging reduced micro-scale pressure variations within the package headspace, thereby enhancing moisture retention by limiting convective and diffusive mass transfer through the anti-fog packaging film. Overall, the integrated experimental-numerical framework provides a reliable predictive tool for analyzing heat and mass transfer in packaged fresh produce and offers practical insights for optimizing mushroom storage conditions and packaging design.

本研究采用实验测量和数值模拟相结合的方法，研究了在4°C和25°C两种温度下，改良和非改良气氛包装下双孢蘑菇（Agaricus bisporus）贮藏期间的水分动态。实验结果表明，在10天的储存期内，平均水分减少约11%，在环境温度和非改性气氛条件下，损失明显更高。在COMSOL中开发的多物理场模型，耦合层流气流、湿空气中的传热和多孔介质中的水分输送，使用实验水分数据进行验证，并预测水分减少约16%，捕捉到观察到的指数衰减趋势。模拟进一步表明，改性大气包装减少了包装顶空内的微尺度压力变化，从而通过限制通过防雾包装膜的对流和扩散传质来增强保湿性。综上所述，该综合实验-数值框架为分析包装新鲜农产品的传热传质提供了可靠的预测工具，并为优化蘑菇的储存条件和包装设计提供了实用的见解。

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引用次数: 0

Suppression of bacterial wilt disease in Japanese pepper Capsicum annuum L. using compost prepared from sewage sludge containing antagonistic microorganisms 利用含有拮抗微生物的污泥堆肥抑制辣椒青枯病的研究

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae

Pub Date : 2026-01-24 DOI: 10.1016/j.scienta.2026.114641

Hasani H Wijesingha , Masashi Miyahara , Hazuki Kurashita , Takahiro Watari , Takashi Yamaguchi , Masashi Hatamoto

Bacterial wilt disease, caused by Ralstonia solanacearum, occurs due to repeated cultivation of vegetables from the same plant family, creating a condition known as continuous cropping disorder. Beneficial soil microbes, particularly those from the Bacillus genus, are critical in enhancing plant health and suppressing soil-borne diseases. This study investigated the effects of a test compost formulated by combining cattle manure compost, rice bran, and dehydrated municipal sewage sludge as a sustainable approach for controlling R. solanacearum in a green chili pepper (Capsicum annuum) cultivation field, where bacterial wilt was prevalent. Nine antagonistic bacterial strains were isolated from the test compost and identified via 16S rRNA gene sequencing as predominantly Bacillus species (B. subtilis, B. velezensis, B. timonensis), along with Streptomyces, Lysinibacillus, and Fictibacillus, with biocontrol mechanisms including antibiotic production and plant growth promotion. The experimental design was compared with untreated control plots with varying application rates of the test compost (0, 3, and 6 kg/m²) over two consecutive growing seasons. In the second season, plots treated with 6 kg/m² of compost showed a 93% reduction in disease severity and a corresponding 90% decrease in R. solanacearum abundance, compared to untreated controls. This study highlights the potential of sewage sludge-derived compost as a sustainable agricultural practice to combat bacterial wilt while establishing resilient microbial communities.

由青枯病引起的细菌性萎蔫病是由于重复种植同一植物科的蔬菜而发生的，造成了一种被称为连作失调的情况。有益的土壤微生物，特别是来自芽孢杆菌属的微生物，在增强植物健康和抑制土壤传播疾病方面至关重要。本文研究了牛粪堆肥、米糠和脱水城市污水污泥混合配制的试验堆肥，在青椒（Capsicum annuum）青椒（Capsicum）枯萎病流行的栽培田中，作为一种可持续控制茄枯病菌的方法。从试验堆肥中分离到9株拮抗细菌，通过16S rRNA基因测序鉴定为芽孢杆菌属（Bacillus subtilis， B. velezensis， B. timonensis）、链霉菌属（Streptomyces）、赖氨酸芽孢杆菌属（Lysinibacillus）和芽孢杆菌属（Fictibacillus），主要为芽孢杆菌属，其生物防治机制包括产生抗生素和促进植物生长。将试验设计与未处理的对照地块进行比较，这些地块在连续两个生长季节中施用不同的试验堆肥（0、3和6 kg/m²）。在第二季，与未经处理的对照相比，施用6 kg/m²堆肥的地块的病害严重程度降低了93%，相应的，茄枯病菌丰度降低了90%。这项研究强调了污水污泥堆肥作为一种可持续农业实践的潜力，可以在建立弹性微生物群落的同时对抗细菌性枯萎病。

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引用次数: 0

Enhancing tomato tolerance to low phosphorus stress through exogenous nano-silicon application 外源纳米硅提高番茄对低磷胁迫的耐性

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae

Pub Date : 2026-01-23 DOI: 10.1016/j.scienta.2026.114635

Haoting Chen , Mengzhu Xu , Ting Tao, Yongjun Zhu, Binchuan Chang, Yu Shi, Jin Xu, Yi Zhang

Phosphorus is essential for plant growth, but excessive fertilizer may deplete phosphate rock reserves and cause environmental issues like eutrophication. Silicon has been reported to improve phosphorus use efficiency, yet the physiological and morphological responses of plants to low-phosphorus (LP) stress across developmental stages remain insufficiently characterized. In this study, we investigated the impact of ionic and nano-silicon foliar sprays (20 and 100 mg·L⁻¹) on the LP stress responses of hydroponically grown tomato seedlings. LP stress hindered seedling growth, elevated lipid peroxidation, and impaired carbohydrate metabolism, leading to altered fruit morphology and quality. Among all treatments, 100 mg·L⁻¹ nano-silicon most effectively reduced ROS accumulation through enhanced antioxidant enzyme activities. It also optimized carbohydrate metabolism by upregulating sucrose synthase and invertase activities, thereby promoting organic matter accumulation and allocation. These improvements increased tomato yield by ∼19 % under LP stress. Fruit quality was also enhanced, with soluble sugar, sucrose, and vitamin C contents increasing by ∼50 %, ∼70 %, and ∼47 %, respectively. Overall, these findings demonstrate that exogenous foliar application of nano-silicon represents a promising agronomic strategy for improving the tolerance of tomato seedlings and fruit quality under LP conditions.

磷对植物生长至关重要，但过量的肥料可能会耗尽磷矿储量，并导致富营养化等环境问题。硅可以提高磷的利用效率，但植物对低磷胁迫的生理和形态反应在发育阶段的研究还不够充分。在这项研究中，我们研究了离子硅和纳米硅叶面喷剂（20和100 mg·L - 1）对水培番茄幼苗LP胁迫反应的影响。低脂胁迫抑制了幼苗生长，提高了脂质过氧化和碳水化合物代谢，导致果实形态和品质发生改变。在所有处理中，100 mg·L -纳米硅通过增强抗氧化酶活性最有效地减少ROS积累。通过上调蔗糖合酶和蔗糖转化酶活性，优化碳水化合物代谢，促进有机质积累和分配。这些改良措施使LP胁迫下的番茄产量提高了19%。果实品质也得到了改善，可溶性糖、蔗糖和维生素C含量分别提高了~ 50%、~ 70%和~ 47%。总的来说，这些研究结果表明，在LP条件下，外源叶面施用纳米硅是一种很有前途的农艺策略，可以提高番茄幼苗的耐受性和果实质量。

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引用次数: 0

Vvgai1 mutation impacts both organogenesis and functioning in Vitis labruscana microvines Vvgai1突变对葡萄的器官发生和功能都有影响

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae

Pub Date : 2026-01-23 DOI: 10.1016/j.scienta.2026.114643

Jana Kändler , Luciana Wilhelm de Almeida , Laurent Torregrosa

In order to develop new genotypes to explore grapevine adaptation to climate, we introduced the Vvgai1 mutation in V. vinifera x V. labrusca hybrids. Vvgai1 mutation induced a strong miniaturisation of leaf and shoot length, as a result of a significant reduction of internodes size and an increase of the phyllochron. The lignification of the main shoots was delayed in the microvines in comparison to their macrovine counterparts while the leaf C assimilation rate was not impacted by the mutation. The shift from the alternate spiral (juvenile) to distichous (adult) phyllotaxis and the appearance of the first tendril occurred at lower node rank in macrovines. However, while macrovines did not produce any reproductive organs during the first vegetative cycle, microvines displayed the first perfect flowers on the main shoot from the internode 18, only a few months after embryo rescue and acclimation. The segregation of the sex type confirmed that the sex determining locus of the V. labrusca cv. Isabella is heterozygous. Conversely, the proportion of opposite-to-leaves organs following a labrusca- versus vinifera-type distribution suggested a more complex genetic determinism for this trait. These experiments provide a new set of microvine genotypes and phenotypic data for studying the response of V. labruscana to abiotic and biotic factors and open new questions about how phytohormones control the development of the grapevine liana.

为了开发新的基因型来探索葡萄对气候的适应性，我们将Vvgai1突变引入葡萄与葡萄的杂交品种中。Vvgai1突变导致叶片和茎长明显缩小，节间大小明显减小，叶长增加。与大藤相比，微藤主茎的木质化延迟，而叶片C的同化速率不受突变的影响。从交替螺旋状（幼体）到二列状（成体）叶状排列的转变和第一卷须的出现发生在大藤较低的节级。然而，大藤在第一次营养循环中没有产生任何生殖器官，而小藤在胚胎抢救和驯化几个月后，从节间18开始，在主茎上展示了第一批完美的花朵。性别类型的分离证实了雌雄同体的性别决定位点。伊莎贝拉是杂合子。相反，相对于叶片的器官的比例，在唇形与葡萄形的分布表明，这一特征有更复杂的遗传决定论。这些实验为研究葡萄藤本植物对非生物和生物因子的响应提供了一套新的基因型和表型数据，并为植物激素如何控制葡萄藤本植物的发育提供了新的问题。

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引用次数: 0

Integrative identification of regulatory genes for potato root development through GWAS, QTL mapping, and transcriptome analysis 通过GWAS、QTL定位和转录组分析综合鉴定马铃薯根系发育调控基因

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae

Pub Date : 2026-01-22 DOI: 10.1016/j.scienta.2026.114627

Jing Yang , Die Jiang , Xinqi Li , Qinggang Liao , Xiaoyan Duan , Yichao Li , Fang Zhao , Wenyi Wang , Wei Tang , Canhui Li

Well-developed root systems are crucial for ensuring high and stable crop yields. Potato (Solanum tuberosum L.) is a typical shallow-rooted crop, making the improvement of root traits to enhance its production potential a significant research objective. This study employed a hydroponic system to investigate potato root phenotypes. Utilizing a natural population composed of 214 diploid potato germplasm resources, we conducted a genome-wide association study. This analysis identified 137 significant single nucleotide polymorphism (SNP) loci associated with root traits, among which 12 were consistently detected across two independent environments. Furthermore, to complement the GWAS, an F₂ mapping population consisting of 170 individuals was developed from a cross between two diploid lines, HD5 and M9, which exhibit contrasting root phenotypes and possess highly homozygous genomes. Whole-genome resequencing was performed on the parental lines and the F₂ population for genotyping. This enabled the construction of a high-density genetic linkage map comprising 4646 Bin markers. Subsequent quantitative trait locus (QTL) mapping using this map identified 38 genetic loci associated with root-related traits, with 16 loci detected in more than one analysis. Comparative analysis of the GWAS and QTL mapping results revealed 11 co-localized loci. To effectively prioritize candidate genes, we focused on 8 reliable loci that were both co-localized by GWAS and QTL mapping and consistently detected across environments. Transcriptome sequencing analysis of the two parental lines (HD5 and M9) identified 139 differentially expressed genes (DEGs) within these reliable loci. Among these DEGs, DM8C01G32920, DM8C02G29270, DM8C02G30060, DM8C03G31680, DM8C05G06230, DM8C09G02710, and DM8C09G21900 emerged as the most promising candidate genes potentially involved in potato root development.

发达的根系对确保作物高产和稳定至关重要。马铃薯（Solanum tuberosum L.）是一种典型的浅根作物，改善马铃薯根系性状以提高其生产潜力是马铃薯研究的重要目标。本研究采用水培系统对马铃薯根系表型进行了研究。利用214个马铃薯二倍体种质资源组成的自然群体，进行了全基因组关联研究。该分析鉴定出137个与根性状相关的显著单核苷酸多态性（SNP）位点，其中12个位点在两个独立的环境中一致检测到。此外，为了补充GWAS，由两个二倍体系HD5和M9杂交开发了一个由170个个体组成的F2定位群体，这两个系表现出截然不同的根表型，具有高度纯合的基因组。对亲本系和F2群体进行全基因组重测序进行基因分型。这使得构建包含4646个Bin标记的高密度遗传连锁图谱成为可能。随后利用该图谱进行数量性状位点（QTL）定位，鉴定出38个与根相关性状相关的遗传位点，其中16个位点在多个分析中被检测到。GWAS和QTL定位结果比较分析发现11个共定位位点。为了有效地确定候选基因的优先级，我们将重点放在了8个可靠的基因座上，这些基因座既通过GWAS和QTL定位，又在不同环境中被一致检测到。对两个亲本系（HD5和M9）的转录组测序分析在这些可靠的位点中鉴定出139个差异表达基因（DEGs）。在这些DEGs中，DM8C01G32920、DM8C02G29270、DM8C02G30060、DM8C03G31680、DM8C05G06230、DM8C09G02710和DM8C09G21900被认为是最有希望参与马铃薯根系发育的候选基因。

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引用次数: 0