Slovenian Veterinary Research最新文献

{"title":"AVIAN CRYPTOSPORIDIOSIS: A SIGNIFICANT PARASITIC DISEASE OF PUBLIC HEALTH HAZARD","authors":"W. A. Abd El-Ghany","doi":"10.26873/svr-1354-2022","DOIUrl":"https://doi.org/10.26873/svr-1354-2022","url":null,"abstract":"Abstract: Cryptosporidiosis is one of the most important zoonotic parasitic diseases affecting a wide range of host species. The disease is widely distributed all over the world. Cryptosporidium species can affect different avian hosts, causing severe economic losses. The severity of avian cryptosporidiosis symptoms vary from asymptomatic disease to severe enteric and/or respiratory manifestations with high mortality. Diagnosis of Cryptosporidium infection is mainly based on microscopic detection of oocysts, serological methods, or molecular techniques to identify different Cryptosporidium species. Humans and animals are highly susceptible to infection by different Cryptosporidium species as a result of the ingestion of contaminated food and water by oocysts or direct contact with infected hosts. Different prevention and control strategies have been applied either in the surrounding environment or for the infected animals, birds, and humans. Therefore, this review article was designed to shed light on avian cryptosporidiosis species and its distribution, susceptibility and infection, clinical pictures, laboratory diagnosis, zoonotic importance in humans, and prevention and control strategies.Key words: avian; control; Cryptosporidium; diagnosis; humanKRIPTOSPORIDIOZA PTIC: POMEMBNA PARAZITSKA BOLEZEN, NEVARNA ZA JAVNO ZDRAVJEIzvleček: Kriptosporidioza je ena najpomembnejših zoonotskih parazitskih bolezni, ki prizadene številne gostiteljske vrste. Bolezen je razširjena po vsem svetu. Vrste Cryptosporidium lahko prizadenejo različne ptičje gostitelje in povzročijo veliko gospodarsko škodo. Resnost simptomov kriptosporidioze pri pticah je različna, od asimptomatične bolezni do hudih črevesnih in/ali dihalnih znakov z visoko smrtnostjo. Diagnoza okužbe s parazitom Cryptosporidium temelji predvsem na mikroskopskem odkrivanju oocist, seroloških metodah ali molekularnih tehnikah za identifikacijo različnih vrst povzročitelja. Ljudje in živali so zelo dovzetni za okužbo z različnimi vrstami Cryptosporidium, ki so posledica zaužitja kontaminirane hrane ali vode z oocistami ali neposrednega stika z okuženimi gostitelji. Za preprečevanje in nadzor bolezni pri okuženih živalih, pticah in ljudeh ali v njihovi okolici so bile uporabljene različne strategije. Namen tega preglednega članka je bil zato razjasniti vrste povzročiteljev in razširjenost kriptosporidioze ptic, dovzetnost za okužbo in način prenosa ter klinično sliko, laboratorijsko diagnostiko, zoonotski pomen in strategije preprečevanja in nadzora bolezni.Ključne besede: ptičji; Cryptosporidium; diagnoza; človeški","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44203745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANTIMICROBIAL RESISTANCE AND VIRULENCE-ASSOCIATED GENES OF AER-OMONADS ISOLATED FROM LAKE MANZALA WATER AND WILD NILE TILAPIA: IMPLICATIONS TO PUBLIC HEALTH AND THE LAKE MICROBIAL COMMUNITY","authors":"H. Eid, H. El-Mahallawy, H. Elsheshtawy, A. Shalaby, M. Shetewy, N. Eidaroos","doi":"10.26873/svr-1348-2022","DOIUrl":"https://doi.org/10.26873/svr-1348-2022","url":null,"abstract":"Abstract: This study was conducted to investigate the prevalence, antimicrobial susceptibility, and molecular characterization of Aeromonas spp. from wild Nile tilapia from Lake Manzala and the lake water. Swabs from the surface, gills, and internal organs of apparently healthy Nile tilapia (n =100) and lake water (n = 25) were collected and examined bacteriologically for the presence of Aeromonas spp. The isolates obtained were tested for their susceptibility to 11 antimicrobial agents using the disk diffusion method. The presence of antibiotic resistance genes (blaTEM, sul1, tetA(A), and aadA1) and virulence genes (enterotoxins) (alt, ast, and act) was determined using conventional polymerase chain reaction. Overall, Aeromonas spp. were recovered from 69% of Nile tilapia (Oreochromis niloticus) samples and 80% of water samples. Four types of aeromonads were detected in all the samples examined, namely, A. hydrophila, A. sobria, A. caviae, and A. schubertii, with A. hydrophila predominating in both the fish and the lake water samples. The antimicrobial resistance profiles of the isolates showed very high resistance to ampicillin, penicillin, sulfamethoxazole/trimethoprim, and oxytetracycline and considerable resistance to streptomycin. However, all isolates were sensitive to cefotaxime. Molecular characterization revealed the presence of the act (100%) and alt (37.5%) genes, but ast was not found in any of the isolates. Specific amplification bands of the antimicrobial resistance genes blaTEM, sul1, and tetA(A) were detected in all the tested isolates, whereas aadA1 (12.5%) was found only in one isolate of A. hydrophila from Nile tilapia. The presence of these enterotoxigenic and resistant Aeromonas spp. in the fish and water samples from Lake Manzala could pose a potential public health threat to human consumers and fish handlers in the study area; moreover, these species carry a risk for the transfer of resistance genes to other microbial communities in the lake.Key words: Aeromonas hydrophile complex; enterotoxin genes; antibiotic resistance genes; Oreochromis niloticus; Lake ManzalaPROTIMIKROBNA ODPORNOST IN Z VIRULENCO POVEZANI GENI BAKTERIJE AEROMONAS IZOLIRANIH IZ JEZERA MANZALA IN DIVJE NILSKE TILAPIJE: VPLIVANJE NA JAVNO ZDRAVJE IN MIKROBNO ZDRUŽBOIzvleček: Namen te študije je bil raziskati razširjenost in protimikrobno občutljivost ter molekularno karakterizirati bakterijo Aeromonas spp., izolirane iz divje nilske talapije in vode iz jezera Manzala. Zbrali smo brise površine, škrg in notranjih organov navidezno zdrave nilske tilapije (n = 100) in vzorce vode (n = 25), ki smo jih bakteriološko pregledali na prisotnost bakterije Aeromonas spp. Pridobljene izolate smo testirali na občutljivost za 11 protimikrobnih sredstev z metodo difuzije diska. Prisotnost genov za odpornost proti antibiotikom (blaTEM, sul1, tetA(A) in aadA1) in genov za virulenco (enterotoksini; alt, ast in act) smo določili z običajno verižno reakcijo s polimerazo. Sk","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44431997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FIRST REPORT OF CANINE MYIASIS WITH SHEEP NASAL BOT FLY, Oestrus ovis, IN SLOVENIA","authors":"A. Vergles Rataj, P. Bandelj, V. Erjavec, Darja Pavlin","doi":"10.26873/svr-1331-2021","DOIUrl":"https://doi.org/10.26873/svr-1331-2021","url":null,"abstract":"Abstract: First larval stage (L1) of Oestrus ovis was recovered by flushing of the nasal cavity during rhinoscopy in an urban living dog. The dog was taken to the Small animal clinic after an acute onset of sneezing and bilateral nasal discharge. In Europe, there are sporadic reports of nasal myiasis in dogs caused by sheep bot flies, and the overall prevalence of O. ovis is high in Mediterranean countries. Because of its habitat expansion due to climate change, it should be considered as a differential diagnosis when an animal patient presents with signs of rhinitis in areas bordering the Mediterranean climate. This is the first report of a dog infested by sheep nasal bot fly in Slovenia.Key words: Oestrus ovis; sheep bot fly; nasal myiasis; dog; climate changesPRVI PRIMER PASJE MIAZE Z OVČJIM NOSNIM ZOLJEM, Oestrus ovis, V SLOVENIJIIzvleček: Med rinoskopijo in spiranjem nosne votline, smo pri psu, ki živi v urbanem okolju, ugotovili ličinke prve stopnje (L1) zajedavca Oestrus ovis. Lastniki so psa pripeljali na Kliniko za male živali po akutnem izbruhu kihanja in bilateralnega nosnega izcedka. V Evropi so dokumentirani sporadični primeri nosne miaze pri psih zaradi ovčjega nosnega zolja, O. ovis, in skupna prevalenca ovčjega zajedavca je v mediteranskih državah visoka. Zaradi klimatskih sprememb, se habitat nosnih zoljev čedalje bolj širi, za kar je pomembno O. ovis vključiti v seznam diferencialnih diagnoz pri pacientih s kliničnimi znaki rinitisa tudi na področjih, ki mejijo na mediteransko klimo. To je prvi opisan primer infestacije psa z ovčjim nosnim zoljem v Sloveniji.Ključne besede: Oestrus ovis; ovčja nosni zolj; nosna miaza; pes; podnebne spremembe","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48884876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE FIRST OUTBREAK OF VIRAL ENCEPHALOPATHY AND RETINOPATHY IN FARMED SEA BASS (Dicentrarchus labrax) IN SLOVENIA","authors":"Rosvita Sitar, T. Švara, Aleksandra Grilc Fajfar, Sabina Šturm, Marko Cvetko, I. Fonda, M. Gombač","doi":"10.26873/svr-1205-2021","DOIUrl":"https://doi.org/10.26873/svr-1205-2021","url":null,"abstract":"Abstract: Viral encephalopathy and retinopathy (VER) is considered a serious disease of several marine fish species, caused by RNA virus belonging to the family Nodaviridae, genus Betanodavirus. The disease is spread almost worldwide and causes significant losses among diseased fish. It is characterised by vacuolation of the central nervous system and the retina. In July 2018, behavioural abnormalities i.e. altered swimming, swirling and vertical floating as well as lethargy and anorexia were observed in farmed sea bass (Dicentrarchus labrax) in the Gulf of Piran (Slovenia), associated with significant mortality. The disease initially occurred in juvenile sea bass, but later market-sized fish also became affected. Diseased fish displayed ocular opacity and multifocal skin ulceration on the head. Emaciation in some fish was also evident. Histopathology revealed characteristic vacuolation in the brain and retina. Performing a RT-PCR and RT-qPCR techniques, we have identified and confirmed the presence of betanodavirus nucleic acid in ocular and brain tissues. In addition, concentrations of the causative agent of VER in spleen and kidney did result in significantly higher viral yield than expected. Phylogenetic analysis showed that Slovenian isolate belongs to RGNNV species of betanodaviruses. Based on the clinical signs, gross and typical microscopic lesions and results of molecular analyses, we can conclude that farmed sea bass from the Gulf of Piran were affected with VER. To the best of our knowledge, this is the first report of VER in Slovenia.Key words: viral encephalopathy and retinopathy; betanodavirus; sea bass; histopathology; RT-qPCR PRVI IZBRUH VIRUSNE ENCEFALOPATIJE IN RETINOPATIJE PRI GOJENIH BRANCINIH (Dicentrarchus labrax) V SLOVENIJI Izvleček: Virusna encefalopatija in retinopatija (VER) je nevarna bolezen številnih vrst morskih rib, ki jo povzroča nevrotropni RNA virus iz družine Nodaviridae, rod Betanodavirus. Bolezen je razširjena skoraj po vsem svetu in povzroča visok pogin okuženih rib. Zanjo so značilne vakuole v centralnem živčnem sistemu in retini. Konec julija 2018 so v ribogojnici v Piranskem zalivu pri brancinih opazili nepravilno plavanje, vrtenje in postavljanje v vertikalno smer ter letargijo in neješčnost, brancini so množično poginjali. Bolezen se je najprej pojavila pri mladicah, nato tudi pri konzumnih kategorijah brancinov. Obolele ribe so imele sivo-motna očesna zrkla ter multifokalne kožne razjede na glavi, posamezne so bile shujšane. S histopatološko preiskavo smo ugotovili značilne vakuole v možganih in retini. Z molekularnima metodama RT-PCR in RT-qPCR smo potrdili prisotnost nukleinske kisline betanodavirusa v očesnem zrklu in možganih. Koncentracije virusa, ki so bile signifikantno višje od pričakovanih, smo ugotovili tudi v vranici in ledvicah. Na podlagi kliničnih znakov, makroskopskih in tipičnih histopatoloških sprememb ter rezultatov molekularnih preiskav lahko zaključimo, da so gojeni brancini v ribog","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45912261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MORPHOLOGY AND HISTOLOGY OF THE EURASIAN LYNX (Lynx lynx) PLANUM NASALE","authors":"Hasan Hüseyin Ari, S. Uslu","doi":"10.26873/svr-1356-2021","DOIUrl":"https://doi.org/10.26873/svr-1356-2021","url":null,"abstract":"Abstract: This study reveals the macroscopic and microscopic structures of the Eurasian lynx planum nasale using materials from three dead females obtained from the Sivas Forestry Branch of Agriculture and Forestry Ministry of the Republic of Turkey. To accomplish the purpose, planum nasale was investigated using macroscopic, histological, and scanning electron microscopy (SEM) techniques. The microscopic examination showed that the planum nasale consists of hairless, moist, glabrous skin and resembles a ship anchor with arm, palm, stock, and sickle parts. The planum nasale’s surface is formed by epidermal plates or epidermal ridges, which  were separated from each other by primary and secondary fissures showed in SEM and macroscopic figures. Based on the microscopic examination, the Mercel’s cells and nerve ends are located in the basal sheet of the planum nasale’s epidermal layers. In addition, the pores situated on the surface of the epidermal ridges and the dense connective bundles were settled in the dermal layers, based on the SEM examination.Key words: Eurasian lynx (Lynx rufus); morphology; nasal plane; planum nasaleMORFOLOGIJA IN HISTOLOGIJA SMRČKA EVRAZIJSKEGA RISA (Lynx lynx)Izvleček: V študiji so opisane makroskopske in mikroskopske strukture smrčka evrazijskega risa, ki je bila opravljena s proučevanjem tkiv treh mrtvih samic, ki so jih pridobili s pomočjo gozdarske podružnice Sivas Ministrstva za kmetijstvo in gozdarstvo Republike Turčije. Strukturo smrčka so raziskovali z uporabo makroskopskih, histoloških metod ter uporabe vrstičnega elektronskega mikroskopa (SEM). Mikroskopska preiskava je pokazala, da smrček sestavlja brezdlaka, vlažna, gola koža, ki po obliki spominja na ladijsko sidro. Površinski del smrčka tvorijo epidermalne plošče ali grebeni, ki jih ločujejo primarne in sekundarne razpoke, vidne na makroskopskih slikah in s pomočjo vrstične mikroskopije. Na histoloških preparatih so bile v bazalni plasti smrčka epidermisa opazne Merkelove celice in živčni končiči. S pomočjo metode SEM so v plasti epidermisa pokazali pore, ki se nahajajo na površini epidermalnih grebenov in snope togega fibrilarnega veziva, ki segajo v plast dermisa.Ključne besede: Evrazijski ris (Lynx rufus); morfologija; nosna ravnina; smrček","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42462683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ISOLATION OF LIVE CELLS FROM DIFFERENT MICE TISSUES UP TO NINE DAYS AFTER DEATH","authors":"G. Majdič, Metka Voga, Ana Pleterski","doi":"10.26873/svr-1155-2021","DOIUrl":"https://doi.org/10.26873/svr-1155-2021","url":null,"abstract":"Abstract: Some limited reports suggest that cells can survive in the cadavers for much longer than it was previously thought.  In our study we explored how time after death, tissue type (muscle, brain and adipose tissue), storage temperature of cadavers (4 °C or at room temperature) and form of tissue storage (stored as cadavers or tissue pieces in phosphate buffered saline) affect the success of harvesting live cells from mice after death. Cells were isolated from dead tissues and grown in standard conditions. Some cells were used for RNA extraction and RT² Profiler™ PCR Array for cell lineage identification was performed to establish which lineages the cells obtained from post mortem tissues belong to. Results of our study showed that viable cells can be regularly isolated from muscle and brain tissue 3 days post mortem and with difficulty up to 6 days post mortem. Viable cells from brain tissue can be isolated up to 9 days post mortem. No cells were isolated from adipose tissue except immediately after death. In all instances viable cells were isolated only when tissues were stored at 4 °C. Tissue storage did not affect cell isolation. Isolated cells were progenitors from different germ layers. Our results show that live cells could be obtained from mouse cadavers several days after death.Key words: mouse; cadaver; stem cells; brain; muscle; adipose tissue IZOLACIJA ŽIVIH CELIC IZ RAZLIČNIH TKIV MIŠI DO DEVET DNI PO SMRTI Izvleček: Nekatere raziskave kažejo, da je preživetje celic v truplih precej daljše, kot je bilo znano do sedaj. V naši raziskavi smo proučevali, kako na uspešnost izolacije živih celic po smrti miši vplivajo različen čas izolacije po smrti, vrsta tkiva (mišično, možgansko in maščobno), temperatura shranjevanja trupel ter oblika shranjenega tkiva (kot koščki tkiv ali kot celi kadavri). Izolacija in gojenje celic iz tkiv mrtvih miši sta potekali pod standardnimi pogoji. Da bi ugotovili, katerim celičnim linijam pripadajo izolirane celice, je bil del celic uporabljen za izolacijo RNK in nadaljno uporabo v sistemu identifikacije izvornih celičnih linij z verižno reakcijo s polimerazo v realnem času. Rezultati naše raziskave so pokazali, da je žive celice mogoče izolirati iz mišičnega in možganskega tkiva 3 dni po smrti, pogojno tudi do 6 dni po smrti. Iz možganskega tkiva je bilo žive celice mogoče izolirati tudi do 9 dni po smrti. Iz maščobnega tkiva je bilo celice mogoče izolirati zgolj takoj po smrti, ne pa tudi v kasnejših časovnih intervalih. V vseh primerih so bile celice izolirane samo v primeru shranjevanja tkiv pri 4°C. Oblika shranjenega tkiva na izolacijo celic ni vplivala. Izolirane celice so pripadale različnim zarodnim plastem. Rezultati raziskave so pokazali, da je žive celice iz mišjih trupel mogoče izolirati tudi več dni po smrti.Ključne besede: miš; truplo; matične celice; možgansko tkivo; mišično tkivo; maščobno tkivo","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42208482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OSTEOMYELITIS ON THE CERVICAL VERTEBRAS OF A FREE-LIVING EUROPEAN HEDGEHOG (Erinaceus europaeus) BY Paeniclostridium sordellii","authors":"Andreia Garcês, Vanessa Soeiro, Sara Lóio, Filipe Silva, I. Pires","doi":"10.26873/svr-1107-2020","DOIUrl":"https://doi.org/10.26873/svr-1107-2020","url":null,"abstract":"A free-living European hedgehog (Erinaceus europaeus) adult female was admitted to the Wildlife Rehabilitation Centre of Parque Biológico de Gaia (Portugal), with severe breathing distress and poor body condition. Its neck was displaced 60 degrees caudally. During the post- mortem exam, an abscess in the cervical vertebras was observed. Paeniclostridium sordellii was the agent isolated from the purulent exudate that was removed from the lesion. This is the first reported case of P. sordellii associated osteomyelitis on the cervical vertebras, and the first time that this pathology is describe in this specie associated with this agent.Key words: Erinaceus europaeus; Portugal; Paeniclostridium sordellii; osteomyelitisOSTEOMIELITIS VRATNIH VRETENC PRI PROSTOŽIVEČM EVROPSKEM JEŽU (Erinaceus europaeus), POVZROČEN Z BAKTERIJO Paeniclostridium sordelliiIzvleček: Prosto živeča odrasla samica evropskega ježa (Erinaceus europaeus) je bila sprejeta v Center za rehabilitacijo divjih živali Parque Biológico de Gaia (Portugalska) s hudo dihalno stisko in slabim telesnim stanjem. Njen vrat je bil premaknjen kavdalno za 60 stopinj. Med sekcijo po smrti so opazili absces na vratnih vretencih. Iz gnojnega eksudata lezije je bila izolirana bakteerija Paeniclostridium sordellii. V članku poročamo o prvem zabeleženem primeru osteomielitisa, povezanega s P. sordellii, na vratnih vretencih in o prvem primeru opisa tovrstne patologije pri evropskem ježu, povezani s tem povzročiteljem.Ključne besede: Erinaceus europaeus; Portugalska; Paeniclostridium sordellii; osteomielitis","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":"1 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41952082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GENETIC DIVERSITY OF EGYPTIAN ARABIAN HORSES FROM EL-ZAHRAA STUD BASED ON 14 TKY MICROSATELLITE MARKERS","authors":"M. Sargious, R. El-Shawarby, M. Abo-Salem, E. El-shewy, H. Ahmed, N. Hagag, S. Ramadan","doi":"10.26873/svr-1041-2020","DOIUrl":"https://doi.org/10.26873/svr-1041-2020","url":null,"abstract":"The objectives of this study were, firstly, to conduct genetic characterization of Egyptian Arabian horses based on 14 TKY microsatellite markers, secondly, to investigate the powerfulness of these 14 TKY markers for parentage assignment of Arabian horses. A total of 101 horse samples including (Arabian = 71, Thoroughbred = 19 and Nooitgedacht = 11) were analysed by 14 TKY microsatellite markers. The PCR products were electrophoresed on Genetic analyzer 3500 with the aid of Liz standard. The basic measures of the allele’s size and genetic diversity were computed using bioinformatics software. The polymorphism of the TKY markers across the Arabian population showed moderate values for genetic diversity parameters; number of allele (NA) =8.143, effective number of allele (Ne) = 3.694, observed heterozygosity (HO) = 0.599, expected heterozygosity (HE) = 0.691, polymorphic Information Content (PIC) = 0.636 and Inbreeding coefficient (FIS)= 0.128. The combined probability of exclusion (CPE) value of the 14 TKY microsatellite loci of our Arabian horses was 0.9999. The results from current study confirm the applicability and efficiency of TKY microsatellite panel for evaluating the genetic diversity and parentage assignment of Egyptian Arabian horses.Key words: Arabian horses; genetic diversity; microsatellite; TKY markers GENSKA RAZNOVRSTNOST EGIPČANSKIH KONJ ARABSKE PASME IZ KOBILARNE EL-ZAHRAA NA PODLAGI 14 MIKROSATELITSKIH OZNAK TKY Izvleček: Nameni raziskave so bili genetska karakterizacija egipčanskih konj arabske pasme na podlagi 14 mikrosatelitskih označevalecv TKY ter raziskava moči 14 označevalcev TKY za dodelitev staršev arabskih konj. S pomočjo 14 mikrosatelitskih označevalcev TKY je bilo analiziranih 101 vzorcev konj (arabski = 71, čistokrvni = 19 in konji Nooitgedacht = 11). Produkte PCR so analizirali s pomočjo elektroforeze na genskem analizatorju 3500 s pomočjo Liz standarda. Osnovne mere velikosti alela in genske raznovrstnosti so bile izračunane s pomočjo programske opreme za bioinformatiko. Polimorfizem označevalcev TKY v arabski populaciji je pokazal zmerne vrednosti za parametre genske raznolikosti; število alelov (NA) = 8,143, efektivno število alelov (Ne) = 3,694, opazovana heterozigotnost (HO) = 0,599, pričakovana heterozigotnost (HE) = 0,691, polimorfna informacijska vsebina (PIC) = 0,636 in Inbriding koeficient (FIS) = 0,128. Skupna vrednost verjetnosti izključitve (CPE) 14 mikrosatelitskih lokusov TKY njihovih arabskih konj je bila 0,9999. Rezultati te raziskave potrjujejo uporabnost in učinkovitost mikrosatelitske plošče TKY za oceno genetske raznovrstnosti in starševske pripadnosti egipčanskih arabskih konj.Ključne besede: arabski konji; genska raznolikost; mikrosatelit; markerji TKY","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49461501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FIRST REPORT OF Paenibacillus cineris FROM A BURMESE PYTHON (Python molurus bivittatus) WITH ORAL ABSCESS","authors":"H. Staji, I. A. Tamai, Zahra Zeifati Kafi","doi":"10.26873/svr-1096-2020","DOIUrl":"https://doi.org/10.26873/svr-1096-2020","url":null,"abstract":"<jats:p />","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45436500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE PRESENCE OF PUTATIVE VIRULENCE DETERMINANTS, TETRACYCLINE AND β-LACTAMS RESISTANCE GENES OF Aeromonas SPECIES ISOLATED FROM PET TURTLES AND THEIR ENVIRONMENT","authors":"S. Wimalasena, G. Heo","doi":"10.26873/SVR-1144-2020","DOIUrl":"https://doi.org/10.26873/SVR-1144-2020","url":null,"abstract":"This study aimed to characterize Aeromonas spp. isolated from ten popular species of pet turtles and their environment to evaluate the potential risk of pet turtles as a source of virulence-associated genes, and tetracycline and β-lactams resistance determinants. Presence of eight virulence genes ( ser, aer, exu, lip, fla, ascV, ahyB and gcat ), and tetracycline ( tetA, tetB and tetE ) and β-lactams ( bla TEM , bla SHV , bla OXA and bla CTX-M ) resistance genes were evaluated by conventional PCR assays. The aerA gene showed the highest frequency of occurrence (92%), followed by fla (75%), gcaT (68%), ahyB (59%), ser (39%), lip (37%) and ascV (25%) genes. None of the isolates carried amplicon of DNase-associated exu gene. A. hydrophila, A. dharkensis, A. veronii and A. caviae were carried seven tested virulence genes except for exu while only four virulence genes were detected in A. enteropelogenes . Among the 75 tetracycline-resistant isolates, tetA, tetE and tetB genes were detected in 38, 26 and 6 isolates, respectively. Among the tested β-lactam resistance genes, bla OXA and bla TEM genes were detected in 54% and 36% of β-lactam resistant isolates, respectively. No bla CTX-M and bla SHV genes were detected. Our results indicate that pet turtle-associated aeromonads, exhibiting potential virulence and antimicrobial (tetracycline and β-lactams) resistance genes, may pose a serious health risk to pet turtle owners, particularly to immunocompromised individuals. Key words: Aeromonas spp.; virulence-associated genes; tetracycline resistance; β-lactams resistance; pet turtle PRISOTNOST DETERMINANT ZA DOLOCITEV DOMNEVNE VIRULENCE TER GENOV ZA ODPORNOST NA TETRACIKLIN IN β-LAKTAM VRST Aeromonas IZOLIRANIH IZ LJUBITELJSKIH VRST ŽELV IN IZ NJIHOVEGA OKOLJA Izvlecek: Namen studije je bil dolociti bakterije Aeromonas spp., izolirane iz desetih priljubljenih vrst hisnih želv in njihovega okolja, z namenom ocenjevanja potencialnega tveganje hisnih želv kot vira genov, povezanih z virulenco, ter determinante odpornosti proti tetraciklinom in β-laktamom. Prisotnost osmih virulentnih genov ( ser, aer, exu, lip, fla, ascV, ahyB in gcat ) ter genov za odpornost na tetracikline ( tetA, tetB in tetE ) in β-laktame (b la TEM , bla SHV , bla OXA in bla CTX-M ) je bila ocenjena s konvencionalnimi testi PCR. Najbolj pogost je bil Gen aerA (92 %), sledili so geni fla (75 %), gcaT (68 %), ahyB (59 %), ser (39 %), lip (37 %) in ascV (25 %). Nobeden od izolatov ni imel pomnoženega gena exu , povezanega z DNAzo. A. hydrophila , A. dharkensis , A. veronii in A. caviae so vsebovali sedem testiranih genov virulence, razen exu , medtem ko so bili v A. enteropelogenih odkriti le stirje virulencni geni. Med 75 izolati, odpornimi na tetracikline, so bili geni tetA , tetE in tetB odkriti v 38, 26 oziroma 6 izolatih. Med preizkusenimi geni za odpornost proti β-laktamu so bili geni bla OXA in bla TEM odkriti pri 54 % oziroma 36 % izolatov, odpornih proti β-laktamu. V nobenem vzorcu ","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45116539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}