Soil Biology & Biochemistry最新文献_第3页

Rare and abundant soil microbes coordinate C, N, P, and quorum sensing pathways to destabilize SOC in shrub-encroached marshes 稀有和丰富的土壤微生物协调碳、氮、磷和群体感应途径，使灌木侵占沼泽的有机碳不稳定

IF 10.3 1区农林科学 Q1 SOIL SCIENCE

Soil Biology & Biochemistry

Pub Date : 2026-04-01 Epub Date: 2026-01-12 DOI: 10.1016/j.soilbio.2026.110086

Ziliang Yin , Xin Sun , Tijiu Cai , Xiaoxin Sun

Shrub encroachment disrupts the dynamic balance between soil organic carbon (SOC) input and output in marsh ecosystems, and directly influences SOC accumulation. Traditional paradigms primarily attribute SOC dynamics to plant traits and soil physicochemical properties, whereas emerging evidence indicates underestimated roles of microbial communities in this process. This study used laboratory incubation, ¹³C NMR spectroscopy, and metagenomic sequencing to explore the key factors regulating marsh SOC stock and stability across four shrub encroachment stages in the largest temperate marsh in Northeast China. The results demonstrate that, although shrub encroachment significantly increased potential sources (e.g., marsh plant biomass and carbon stock) of SOC, low carbon quality prevented a substantial increase in SOC stocks and stability. Notably, soil microbial communities were pivotal drivers in regulating SOC dynamics in plant-soil-microbe interactions. Six carbon fixation pathways dominated by abundant and transitional taxa explained only 0.07 % of SOC stock variation, whereas the synergistic interactions between microorganisms and plants or soil had the most significant effect on SOC stocks. In contrast, the variation in SOC stability was primarily attributed to changes in carbohydrate-active enzyme (CAZyme) gene profiles dominated by rare taxa (61.26 %), surpassing the explanatory power of plant traits and soil physicochemical properties. Additionally, rare taxa substantially influenced synergistic interactions among nitrogen cycling, phosphorus cycling, carbon fixation, and CAZyme genes via the quorum sensing (QS) pathway. This study provides novel insights into the effects of plant-soil-microbial interactions on marsh SOC transformation during shrub encroachment, highlighting the potential of rare taxa to release available nutrients and accelerating carbon, nitrogen, and phosphorus cycling.

灌丛入侵破坏了沼泽生态系统土壤有机碳（SOC）输入与输出的动态平衡，直接影响了土壤有机碳的积累。传统的研究范式主要将土壤有机碳动态归因于植物性状和土壤理化性质，而新的研究证据表明，微生物群落在这一过程中的作用被低估了。本研究采用实验室培养、13C核磁共振、宏基因组测序等方法，对东北最大的温带沼泽进行了4个灌木入侵阶段的土壤有机碳储量和稳定性调控。结果表明，尽管灌木入侵显著增加了土壤有机碳的潜在来源（如沼泽植物生物量和碳储量），但低碳质量阻碍了土壤有机碳储量和稳定性的大幅增加。土壤微生物群落是调节植物-土壤-微生物相互作用中有机碳动态的关键驱动因素。以丰富和过渡性类群为主的6种碳固定途径仅解释了0.07%的碳储量变化，而微生物与植物或土壤的协同作用对碳储量的影响最为显著。土壤有机碳稳定性的变化主要归因于碳水化合物活性酶（CAZyme）基因谱的变化，该基因谱以稀有类群为主（61.26%），超过了植物性状和土壤理化性质的解释力。此外，稀有类群通过群体感应（quorum sensing， QS）途径显著影响了氮循环、磷循环、碳固定和CAZyme基因之间的协同相互作用。本研究为灌木侵蚀过程中植物-土壤-微生物相互作用对沼泽有机碳转化的影响提供了新的见解，强调了稀有类群释放有效养分和加速碳、氮、磷循环的潜力。

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引用次数: 0

Additional carbon conversion driven by microbial metabolic limitations in long-term phosphorus-fertilized soil: The role of reactive oxygen species 长期磷肥土壤微生物代谢限制驱动的额外碳转化：活性氧的作用

IF 10.3 1区农林科学 Q1 SOIL SCIENCE

Soil Biology & Biochemistry

Pub Date : 2026-04-01 Epub Date: 2026-01-19 DOI: 10.1016/j.soilbio.2026.110095

Yuanyuan Ding , Na Chen , Bin Jia , Huiqiang Yang , Fuhao Liu , Kangjie Yang , Zhiqiang Wang , Jianjun Qin , Jia Xie , Yingfei Cao , Xueyun Yang , Hanzhong Jia

Reactive oxygen species (ROS) are important drivers of soil carbon (C) turnover, but how ROS production responds to phosphorus (P) application and participates in C turnover remain unclear. Based on a six-year P fertilizer application experiments, we found that P application decreased superoxide (O₂^•−) content by 36 % and 69 % in high- and low-fertility soils, respectively, whereas it increased hydroxyl radical (^•OH) content by 90 % in high-fertility soils, but had minimal impact in low-fertility soils. After gamma sterilization, O₂^•− content and ^•OH content decreased by approximately 54 % and 51 % respectively, indicating the significant role of microorganisms in ROS production. Random forest analysis identified microbial C limitation and P limitation as drivers of O₂^•− and ^•OH production, respectively. Aggravated microbial C limitation with P fertilization may inhibit the biotic O₂^•− generation by reducing the relative abundance of copiotrophic bacteria (primarily Alphaproteobacteria and Betaproteobacteria). Incubation experiments confirmed this inhibitory effect, and demonstrated that the mitigation of microbial P limitation with P fertilization promotes ^•OH production in Fenton-like reactions by increasing the content of surface-adsorbed Fe(II) and Fe(II) in low-crystallinity minerals. The increase in the Fe(II) contents were attributed to iron-reducing microorganism, as confirmed by sterilization experiments. Additionally, incubation experiments further revealed that microbial C–P co-limits has a stronger negative effect on ROS content than a single limitation, which likely explains the lower ROS levels in low-fertility soils with more pronounced C–P co-limitation. ROS quench and addition experiments confirmed that the generated ^•OH with P application in high-fertility soils contributes 11–20 % of CO₂ emissions; whereas P application in low-fertility soils favored soil organic C sequestration by decreasing O₂^•− content and maintaining low ^•OH levels. Overall, the obtained results broaden the understanding of ROS production in soils and provide new insights into carbon turnover in fertilized soil.

活性氧（ROS）是土壤碳(C)转化的重要驱动因素，但活性氧（ROS）的产生如何响应磷(P)的施用并参与C的转化尚不清楚。通过为期6年的磷肥施用试验，我们发现施用磷肥使高肥力土壤和低肥力土壤的超氧化物（O2•−）含量分别降低了36%和69%，而羟基自由基（•OH）含量在高肥力土壤中增加了90%，但对低肥力土壤的影响很小。经过伽马灭菌后，O2•−含量和•OH含量分别下降了约54%和51%，这表明微生物在ROS的产生中发挥了重要作用。随机森林分析发现微生物C限制和P限制分别是O2•−和•OH生产的驱动因素。施磷加剧微生物C限制可能通过降低共同营养细菌（主要是Alphaproteobacteria和Betaproteobacteria）的相对丰度来抑制生物O2•−的产生。培养实验证实了这种抑制作用，并表明施磷肥缓解微生物磷限制通过增加低结晶度矿物中表面吸附铁（II）和铁（II）的含量来促进芬顿样反应中•OH的产生。灭菌实验证实，铁（II）含量的增加是由铁还原微生物引起的。此外，培养实验进一步揭示，微生物C-P共同限制比单一限制对ROS含量的负面影响更强，这可能解释了C-P共同限制更明显的低肥力土壤中ROS水平较低的原因。活性氧猝灭和添加实验证实，高肥力土壤施磷产生的•OH贡献了11 - 20%的CO2排放；而在低肥力土壤中施用磷肥有利于土壤有机碳的固存，通过降低O2•−含量和维持较低的•OH水平。总的来说，获得的结果拓宽了对土壤中活性氧产生的理解，并为施肥土壤的碳周转提供了新的见解。

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引用次数: 0

Rhizosphere carbon flux of eight temperate tree species growing on a common site 在同一地点生长的八种温带树种的根际碳通量

IF 10.3 1区农林科学 Q1 SOIL SCIENCE

Soil Biology & Biochemistry

Pub Date : 2026-04-01 Epub Date: 2026-01-13 DOI: 10.1016/j.soilbio.2026.110083

Timothy J. Fahey, Joseph B. Yavitt

Tree roots are the principal source of stabilized organic matter in forest soils, supplying carbon in the form of detritus (root turnover) and rhizodeposition from living roots (rhizosphere carbon flux; RCF). The magnitude of these processes is highly uncertain owing to the difficulty of measurement in situ in mature forests. We estimated RCF in twelve monospecific forest plantations growing on a common soil in central New York using a root ingrowth ¹³C dilution technique. The plantations included eight tree species, four each with ectomycorrhizal or arbuscular mycorrhizal associations. We hypothesized that RCF would be greater for arbuscular mycorrhizal than ectomycorrhizal tree species and that this contrast in mycorrhizal type would account for most species’ differences. Our estimates of RCF averaged 109 g C m⁻² y⁻¹ in the upper 0–20 cm soil interval, and the estimates differed significantly among tree species with mostly greater values for arbuscular mycorrhizal than ectomycorrhizal tree species. Although a large amount of new carbon was added by RCF, the carbon content of rhizosphere soil was unchanged, suggesting that RCF primed the mineralization of soil organic matter (SOM) in the ingrowth root cores. Our measurements indicate that RCF comprises an average of about 20 % of aboveground net primary production in these forest plantations and suggest that similar amounts of carbon are added to soil by root turnover and RCF.

树根是森林土壤中稳定有机质的主要来源，以碎屑（根周转）和活根的根沉积（根际碳通量；RCF）的形式提供碳。由于难以在成熟森林中就地测量，这些过程的大小极不确定。我们使用根向生长13C稀释技术估算了生长在纽约中部共同土壤上的12个单种森林人工林的RCF。这些人工林包括8种树种，每种树种4种具有外生菌根或丛枝菌根关联。我们假设丛枝菌根树种的RCF大于外生菌根树种，并且这种菌根类型的差异可以解释大多数物种的差异。我们估计的RCF在0 ~ 20 cm土壤间隔上平均为109 g C m−2 y−1，树种之间的估计值存在显著差异，丛枝菌根树种的估计值大多高于外生菌根树种。虽然RCF增加了大量的新碳，但根际土壤的碳含量没有变化，表明RCF启动了长生根芯土壤有机质（SOM）的矿化。我们的测量表明，RCF平均约占这些人工林地上净初级产量的20%，并表明根系周转和RCF向土壤中添加了相似数量的碳。

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引用次数: 0

SOC stabilisation shifts from carbon accumulation in temperate soils to mineral association in subtropical soils 有机碳稳定从温带土壤的碳积累转向亚热带土壤的矿物结合

IF 10.3 1区农林科学 Q1 SOIL SCIENCE

Soil Biology & Biochemistry

Pub Date : 2026-04-01 Epub Date: 2026-01-23 DOI: 10.1016/j.soilbio.2026.110101

Yijia Tang, Thi Kim Anh Tran, Budiman Minasny, Shiva Bakhshandeh, Mingming Du, Nicolas Francos, Yin-Chung Huang, Ho Jun Jang, Wartini Ng, Peipei Xue, Alex McBratney

Soil organic carbon (SOC) is operationally partitioned into particulate organic carbon (POC) and mineral-associated organic carbon (MAOC) to infer soil carbon persistence and sensitivity to disturbance or management. Yet the combined roles of climate, mineralogy, and land use in shaping these fractions remain unresolved across continental gradients. Here, we analysed 249 Australian topsoils from 65 paired sites along a 550 mm rainfall isohyet. Samples were grouped into four climate–texture clusters (Subtropical Coarse, Subtropical Fine, Temperate Coarse, Temperate Fine) to disentangle the effects of thermal and hydrological regimes, soil properties, and land use on SOC partitioning and stabilisation. Subtropical soils consistently exhibited a high proportion of MAOC (fMAOC ≈ 0.8) despite low SOC stocks, reflecting preferential retention of mineral–organic interactions under carbon-limited and water-stressed conditions. In contrast, temperate soils stored greater SOC and POC, indicating higher carbon inputs and slower decomposition. In subtropical fine-textured soils, agriculture elevated fMAOC through microbial activity and nutrient inputs, yet this occurred alongside depleted SOC and POC, highlighting a trade-off between stabilisation efficiency and carbon stock depletion. Across all clusters, land use effects were detectable but secondary to climate and mineral properties. These findings show that temperate and subtropical soils follow contrasting carbon stabilisation pathways: temperate systems store more carbon overall, while subtropical systems allocate a larger share of their carbon to mineral-associated carbon. Our climate–texture framework highlights region-specific management priorities: enhancing mineral–organic interactions through increased root inputs and organic or mineral amendments in subtropical soils, and protecting vulnerable carbon stocks through reduced disturbance and residue retention in temperate systems.

土壤有机碳（SOC）在操作上分为颗粒有机碳（POC）和矿物相关有机碳（MAOC），以推断土壤碳的持久性和对干扰或管理的敏感性。然而，气候、矿物学和土地利用在形成这些部分中的综合作用在整个大陆梯度上仍未得到解决。在这里，我们沿着550毫米降雨等雨量线分析了来自65个成对地点的249个澳大利亚表土。样本被分为4个气候结构集群（亚热带粗、亚热带细、温带粗、温带细），以理清热水文制度、土壤性质和土地利用对有机碳分配和稳定的影响。亚热带土壤在有机碳储量较低的情况下，仍表现出较高的MAOC比例（fMAOC≈0.8），反映出在碳限制和水分胁迫条件下矿物-有机相互作用的优先保留。相比之下，温带土壤的SOC和POC储量更高，表明碳输入更高，分解速度更慢。在亚热带细质土壤中，农业通过微生物活动和养分投入提高了fMAOC，但这与SOC和POC的耗尽同时发生，突出了稳定效率和碳储量枯竭之间的权衡。在所有集群中，土地利用效应都是可检测到的，但次于气候和矿物性质。这些发现表明，温带和亚热带土壤遵循截然不同的碳稳定途径：温带系统总体上储存更多的碳，而亚热带系统将其碳分配给矿物相关碳的比例更大。我们的气候结构框架强调了特定区域的管理重点：通过增加亚热带土壤的根系投入和有机或矿物修正来加强矿物-有机相互作用，并通过减少温带系统的干扰和残留物保留来保护脆弱的碳储量。

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引用次数: 0

The role of bacterial rrn copy number in shaping ecological strategies in soil: Is it time to re-evaluate this functional trait? 细菌rrn拷贝数在塑造土壤生态策略中的作用：是时候重新评估这一功能特性了吗？

IF 10.3 1区农林科学 Q1 SOIL SCIENCE

Soil Biology & Biochemistry

Pub Date : 2026-04-01 Epub Date: 2026-01-19 DOI: 10.1016/j.soilbio.2026.110099

Jaron Adkins , Karen M. Foley , Karen H. Beard , Trisha B. Atwood , Bonnie G. Waring

Developing a mechanistic understanding of how soil microbial communities underlie ecosystem functions has become a pressing need in the face of rapid environmental change. In service of this goal, soil microbial ecologists have sought to develop general principles about the ecological strategies that soil microbes adopt and the functional traits that contribute to those strategies. Among bacteria, the trait most commonly invoked as an indicator for bacterial performance and ecological strategy in soils is bacterial rrn operon copy number (RCN), which is frequently applied as an indicator of a bacterial community's position on a continuum of copiotrophic vs oligotrophic ecological strategies. The use of RCN as a proxy for bacterial ecological strategy is based on the premise that RCN mediates a tradeoff between bacterial growth rate and growth yield efficiency. However, support for RCN in mediating a growth rate-yield tradeoff is limited to a small number of studies performed in culture environments, and there is no evidence for the involvement of RCN in such a tradeoff in real soils. In contrast, emerging evidence suggests that RCN has a positive influence on soil extracellular enzyme activity, indicating that RCN may be a functional trait related to decomposition activity in soils. Here, we draw on new and previously published empirical data as well as simulation modelling to reassess the contribution of RCN to bacterial ecological strategies in soils. We conclude that while RCN is positively associated with bacterial growth rate and soil exoenzyme activity under some resource conditions, its relationship to growth yield efficiency remains unclear. Considering this, we suggest that RCN holds promise for understanding the contribution of soil bacteria to ecosystem functions, but the common application of RCN as an indicator of overall bacterial ecological strategy is inappropriate in light of current evidence.

面对快速的环境变化，发展对土壤微生物群落如何影响生态系统功能的机制理解已成为迫切需要。为了实现这一目标，土壤微生物生态学家试图发展关于土壤微生物采用的生态策略和有助于这些策略的功能特征的一般原则。在细菌中，细菌rrn操纵子拷贝数（RCN）是土壤中细菌表现和生态策略最常用的指标，它经常被用作细菌群落在共养与寡养生态策略连续体中的位置的指标。使用RCN作为细菌生态策略的代理是基于RCN在细菌生长速率和生长产量效率之间进行权衡的前提。然而，支持RCN调节生长速率-产量权衡的研究仅限于在培养环境中进行的少数研究，并且没有证据表明RCN参与实际土壤中的这种权衡。相反，新出现的证据表明，RCN对土壤胞外酶活性有积极影响，表明RCN可能是与土壤分解活性相关的功能性状。在这里，我们利用新的和以前发表的经验数据以及模拟模型来重新评估RCN对土壤中细菌生态策略的贡献。我们得出结论，虽然在某些资源条件下RCN与细菌生长速率和土壤外酶活性呈正相关，但其与生长产量效率的关系尚不清楚。考虑到这一点，我们认为RCN有望理解土壤细菌对生态系统功能的贡献，但根据目前的证据，将RCN作为整体细菌生态策略的指标是不合适的。

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引用次数: 0

How nitrifiers denitrify? 硝化剂如何反硝化？

IF 10.3 1区农林科学 Q1 SOIL SCIENCE

Soil Biology & Biochemistry

Pub Date : 2026-04-01 Epub Date: 2026-01-20 DOI: 10.1016/j.soilbio.2026.110098

Kaihang Zhang, Lei Cheng

The sources of nitrous oxide (N₂O), a potent and long-lived greenhouse gas, remain highly uncertain. This uncertainty arises largely from limited understanding of nitrifier denitrification, the reduction of nitrite by ammonia oxidizing bacteria (AOB) (Zhu et al., 2013). Here, we propose a novel conceptual framework to address the long-standing question of whether AOB could persist via nitrifier denitrification under hypoxic and/or anoxic conditions. We present an analysis of recent advances in nitrite and nitric oxide (NO) reductase enzymology (Murali et al., 2024) which suggests that AOB could utilize heme-copper-containing NO reductase (sNOR)-mediated nitrifier denitrification to generate proton motive force and conserve energy during anaerobic respiration. Our phylogenetic analysis further shows that sNOR is present in nearly 96 % of sequenced AOB genomes. We therefore suggest that sNOR-mediated nitrifier denitrification may represent a substantial, and likely increasing, contribution to global N₂O emissions, particularly in aquatic and lowland ecosystems under future climate change-induced global deoxygenation conditions.

一氧化二氮（N2O）是一种强效且长寿命的温室气体，其来源仍然高度不确定。这种不确定性主要源于对硝化菌反硝化作用的有限理解，即氨氧化细菌（AOB）对亚硝酸盐的还原（Zhu et al., 2013）。在这里，我们提出了一个新的概念框架来解决长期存在的问题，即在缺氧和/或缺氧条件下，AOB是否可以通过硝化器反硝化持续存在。我们对亚硝酸盐和一氧化氮还原酶（NO）酶学的最新进展进行了分析（Murali et al., 2024），表明AOB可以利用含血红素-铜的NO还原酶（sNOR）介导的硝化物反硝化作用来产生质子动力并在厌氧呼吸过程中保存能量。我们的系统发育分析进一步表明，sNOR存在于近96%的测序AOB基因组中。因此，我们认为，在未来气候变化引起的全球脱氧条件下，snorr介导的硝化物反硝化作用可能对全球N2O排放做出了实质性的贡献，并且可能会增加，特别是在水生和低地生态系统中。

引用次数: 0

Long-term warming and vegetation change have no impact on microbial resistance to drought, but destabilise microbial communities and microbially-mediated functions 长期的气候变暖和植被变化对微生物抗旱能力没有影响，但会破坏微生物群落的稳定性和微生物介导的功能

IF 10.3 1区农林科学 Q1 SOIL SCIENCE

Soil Biology & Biochemistry

Pub Date : 2026-04-01 Epub Date: 2026-01-21 DOI: 10.1016/j.soilbio.2026.110100

Ellen L. Fry , Amy L. Evans , Deborah Ashworth , Ana Soto , Juntao Wang , Nick Ostle , Brajesh K. Singh , Richard D. Bardgett

Climate change presents multiple stresses to ecosystems that operate over different timescales, such as long-term warming and short-term drought. It is well established that soil microbial communities are highly responsive to individual stresses, but how they respond combined warming and drought, and how factors such as vegetation change moderate responses, remains uncertain. Here we tested whether long-term passive warming modifies the resistance (amplitude of response) and resilience (degree and duration of recovery) of soil microbial communities to short-term drought. We also tested whether warming effects on microbial resilience to drought are moderated by vegetation composition, and specifically the presence of ericaceous dwarf shrubs, the dominant vegetation type of peatland. This was tested using soil from a nine-year warming and vegetation manipulation experiment established on blanket peatland in northern England. We completed a subsequent laboratory study designed to quantify resistance and resilience of microbial communities and microbial-mediated functions to drought. Neither long-term warming nor shrub removal impacted the resistance of microbial communities to drought. However, resilience of bacterial diversity to drought was decreased by warming (fold change 0.38) and shrub removal (fold change 0.27). Notably the interaction between warming and shrub removal resulted in higher resilience of bacterial diversity than individual treatments (fold change 0.58; warming x shrub removal: p = 0.008). Further, warming and shrub removal individually increased the diversity of fungal communities, and reduced resilience of fungal diversity to drought (fold change of warmed against unwarmed 0.11, shrub removal against control 0.39, combination against control 0.59; warming x shrub removal p = 0.006). Warming also strongly decreased resilience, but not resistance, of nitrogen-based functions to drought, although shrub removal dampened this effect. Our findings demonstrate potential for long-term warming and vegetation change to modify microbial responses to extreme drought events, with implications for peatland carbon and nitrogen cycling under future climate scenarios.

气候变化给在不同时间尺度上运行的生态系统带来多重压力，如长期变暖和短期干旱。众所周知，土壤微生物群落对个体胁迫具有高度的响应，但它们如何对增温和干旱共同作用作出响应，以及植被等因素如何改变适度的响应，仍不确定。在这里，我们测试了长期被动变暖是否会改变土壤微生物群落对短期干旱的抗性（响应幅度）和弹性（恢复程度和持续时间）。我们还测试了气候变暖对微生物抗旱能力的影响是否受到植被组成的调节，特别是泥炭地主要植被类型白垩矮灌木的存在。这是在英格兰北部地毯式泥炭地进行的一项为期9年的变暖和植被控制实验中的土壤进行的测试。我们随后完成了一项实验室研究，旨在量化微生物群落对干旱的抗性和恢复力以及微生物介导的功能。长期增温和灌木砍伐都没有影响微生物群落的抗旱性。增温（倍数变化0.38）和灌木砍伐（倍数变化0.27）降低了细菌多样性的抗旱能力。值得注意的是，与单独处理相比，增温和灌木去除之间的相互作用导致细菌多样性的恢复力更高（倍数变化0.58；增温x灌木去除：p = 0.008）。增温和灌木去除分别增加了真菌群落的多样性，降低了真菌多样性对干旱的适应能力（增温与未增温的倍数变化为0.11，增温与对照的倍数变化为0.39，增温与对照的倍数变化为0.59，增温与灌木去除的倍数变化p = 0.006）。变暖也强烈降低了氮基功能对干旱的恢复力，而不是抗性，尽管灌木的移除抑制了这种影响。我们的研究结果表明，长期的气候变暖和植被变化可能会改变微生物对极端干旱事件的反应，这对未来气候情景下泥炭地的碳和氮循环有影响。

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引用次数: 0

Does soil inoculation truly steer the composition of native soil communities? 土壤接种真的能控制原生土壤群落的组成吗？

IF 10.3 1区农林科学 Q1 SOIL SCIENCE

Soil Biology & Biochemistry

Pub Date : 2026-04-01 Epub Date: 2026-01-29 DOI: 10.1016/j.soilbio.2026.110105

Yuhui Li , Yingbin Li , Xu Han , Qi Li , T. Martijn Bezemer

Soil inoculation is a promising tool for restoring degraded ecosystems, that is based on the assumption that introduced soil communities establish in the new environment and create positive feedback with new plant communities. However, we lack empirical evidence on the extent to which the soil community present in the relatively small amount of inoculated soil can truly alter the recipient or native soil community in the field. For the first time, we measured the soil community in both the inoculated and in the underlying soil layers in a whole-soil inoculation experiment in a restoration grassland. Within the inoculated layer, nematode and bacterial communities became increasingly similar to the donor soil community. However, in the soil layer beneath the inoculated layer, the similarity of the native bacterial and nematode communities to the donor community did not change significantly over time. Only the native fungal community gradually shifted towards the community present in the donor meadow. Our study provides conclusive field evidence that soil inoculation can restructure soil biota at the recipient site, but this effect is largely restricted to the inoculated soil layer. This challenges the commonly held assumption that soil inoculation drives changes in native soil communities through positive plant-soil feedbacks.

土壤接种是恢复退化生态系统的一种很有前景的工具，其基础假设是引入的土壤群落在新环境中建立并与新的植物群落产生正反馈。然而，我们缺乏经验证据表明，在相对少量的接种土壤中存在的土壤群落在多大程度上能够真正改变田间接受者或原生土壤群落。在恢复草地全土壤接种试验中，首次对接种层和下垫层土壤群落进行了测量。在接种层内，线虫和细菌群落与供体土壤群落越来越相似。然而，在接种层以下的土层中，原生细菌和线虫群落与供体群落的相似性没有随时间发生显著变化。只有原生真菌群落逐渐向供体草甸中存在的群落转移。我们的研究提供了确凿的田间证据，证明土壤接种可以重构受训地的土壤生物群，但这种作用主要局限于接种层。这挑战了普遍持有的假设，即土壤接种通过积极的植物-土壤反馈驱动原生土壤群落的变化。

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引用次数: 0

Biodiversity co-variation patterns in a range of soil organism taxa across highly contrasting ecosystems 在高度对比的生态系统中一系列土壤生物类群的生物多样性共变模式

IF 10.3 1区农林科学 Q1 SOIL SCIENCE

Soil Biology & Biochemistry

Pub Date : 2026-04-01 Epub Date: 2026-01-14 DOI: 10.1016/j.soilbio.2026.110093

Axelle Tortosa , Grégoire T. Freschet , Jean Trap , Alain Brauman , Yvan Capowiez , Sylvain Coq , Jim Félix-Faure , Nathalie Fromin , Laure Gandois , Maritxu Guiresse , Raoul Huys , Antoine Lecerf , Jean-Marc Limousin , Alexandru Milcu , Johanne Nahmani , Agnès Robin , José Miguel Sánchez-Pérez , Sabine Sauvage , Tiphaine Tallec , Claire Wittling , Stephan Hattenschwiler

Soil biodiversity as a critical component of terrestrial ecosystems and their functioning varies across spatial scales and environmental conditions. However, it remains unclear whether and how biodiversity patterns co-vary among different soil taxa across ecosystems.

In this study, we compared diversity patterns of plants, earthworms, nematodes, bacteria, and fungi, as five major groups of soil organisms, across six strongly contrasting ecosystems ranging from mountain peatland to crop fields, including within-ecosystem variation in soil moisture. We hypothesized co-variation in taxonomic richness (alpha diversity) and composition (beta diversity) of multiple groups of soil organisms across ecosystems, moisture conditions and spatial scales.

In partial contrast to our initial hypothesis, co-variation in the taxonomic richness among these groups was limited, though significant positive associations were found among bacteria, fungi, and earthworms across all sites. Plant diversity showed distinct associations with soil organism diversity, particularly with earthworms and bacteria, highlighting above–belowground biodiversity linkages. Beta diversity showed substantial co-variation among all soil organism groups, reflecting a spatial coupling of their communities that was influenced by differences in soil moisture conditions. These patterns were more pronounced in near-natural and no-till agroecosystems compared to conventional agricultural systems. Our results highlight that ecosystem type shapes broad-scale taxonomic richness, while local soil moisture critically influences soil biodiversity and spatial community composition, emphasizing the multi-scale drivers of soil biodiversity.

土壤生物多样性是陆地生态系统的重要组成部分，其功能因空间尺度和环境条件而异。然而，生物多样性模式是否以及如何在不同生态系统的不同土壤分类群之间共同变化仍不清楚。在这项研究中，我们比较了植物、蚯蚓、线虫、细菌和真菌这五大类土壤生物，在从山地泥炭地到农田的六个强烈对比的生态系统中的多样性模式，包括生态系统内土壤湿度的变化。我们假设在不同的生态系统、湿度条件和空间尺度上，不同土壤生物类群的分类丰富度（α多样性）和组成（β多样性）是共同变异的。与我们最初的假设部分相反，这些类群之间的分类丰富度的共同变异是有限的，尽管在所有地点的细菌、真菌和蚯蚓之间发现了显著的正相关。植物多样性与土壤生物多样性表现出明显的相关性，特别是与蚯蚓和细菌的相关性，突出地表上的生物多样性联系。β多样性在所有土壤生物类群中均表现出显著的共变，反映了受土壤水分条件差异影响的空间耦合。与传统农业系统相比，这些模式在近自然和免耕农业生态系统中更为明显。研究结果表明，生态系统类型决定了大尺度的分类丰富度，而当地土壤湿度对土壤生物多样性和空间群落组成具有重要影响，强调了土壤生物多样性的多尺度驱动因素。

{"title":"Biodiversity co-variation patterns in a range of soil organism taxa across highly contrasting ecosystems","authors":"Axelle Tortosa , Grégoire T. Freschet , Jean Trap , Alain Brauman , Yvan Capowiez , Sylvain Coq , Jim Félix-Faure , Nathalie Fromin , Laure Gandois , Maritxu Guiresse , Raoul Huys , Antoine Lecerf , Jean-Marc Limousin , Alexandru Milcu , Johanne Nahmani , Agnès Robin , José Miguel Sánchez-Pérez , Sabine Sauvage , Tiphaine Tallec , Claire Wittling , Stephan Hattenschwiler","doi":"10.1016/j.soilbio.2026.110093","DOIUrl":"10.1016/j.soilbio.2026.110093","url":null,"abstract":"<div><div>Soil biodiversity as a critical component of terrestrial ecosystems and their functioning varies across spatial scales and environmental conditions. However, it remains unclear whether and how biodiversity patterns co-vary among different soil taxa across ecosystems.</div><div>In this study, we compared diversity patterns of plants, earthworms, nematodes, bacteria, and fungi, as five major groups of soil organisms, across six strongly contrasting ecosystems ranging from mountain peatland to crop fields, including within-ecosystem variation in soil moisture. We hypothesized co-variation in taxonomic richness (alpha diversity) and composition (beta diversity) of multiple groups of soil organisms across ecosystems, moisture conditions and spatial scales.</div><div>In partial contrast to our initial hypothesis, co-variation in the taxonomic richness among these groups was limited, though significant positive associations were found among bacteria, fungi, and earthworms across all sites. Plant diversity showed distinct associations with soil organism diversity, particularly with earthworms and bacteria, highlighting above–belowground biodiversity linkages. Beta diversity showed substantial co-variation among all soil organism groups, reflecting a spatial coupling of their communities that was influenced by differences in soil moisture conditions. These patterns were more pronounced in near-natural and no-till agroecosystems compared to conventional agricultural systems. Our results highlight that ecosystem type shapes broad-scale taxonomic richness, while local soil moisture critically influences soil biodiversity and spatial community composition, emphasizing the multi-scale drivers of soil biodiversity.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"215 ","pages":"Article 110093"},"PeriodicalIF":10.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in synergistic biotic-abiotic dehalogenation in soil: FemSn-mediated electron transfer and microbial metabolic network regulation 土壤生物-非生物协同脱卤研究进展：femsn介导的电子转移和微生物代谢网络调控

IF 10.3 1区农林科学 Q1 SOIL SCIENCE

Soil Biology & Biochemistry

Pub Date : 2026-04-01 Epub Date: 2026-01-10 DOI: 10.1016/j.soilbio.2026.110084

Boyu Jia , Siyu Zhang , Ningning Wu , Liqi Cai , Zhongdan Li , Shanquan Wang

The integration of biotic- and abiotic-dehalogenation strategies offers a transformative approach to remediating organohalide-contaminated soils, harnessing the synergistic benefits of biological selectivity and abiotic efficiency. However, challenges in modulating electron flux partitioning at biotic-abiotic interfaces and in reconstructing adaptive microbial metabolic networks continue to impede practical implementation.

This review comprehensively synthesizes recent advances in synergistic biotic-abiotic strategies for removing organohalide pollutants from contaminated sites, with a particular emphasis on iron-sulfur mineral species (Fe_mS_n)-mediated electron transfer mechanisms and the regulation of microbial metabolic networks. In this framework, electrons are transferred via surface Fe–S active sites on mineral phases, enabling electron tunneling at interfaces to microbial extracellular carriers and soluble redox mediators that coordinate flux in soil dechlorination system. This review begins with respiratory electron transport chains in organohalide-respiring bacteria (OHRB), while highlighting evolutionary trade-offs in electron carrier utilization and energy conservation. It then explores microbial interactions, showing how crystallographic defect engineering enhances enzymatic activation via electron tunneling and mitigates nanomaterial toxicity. Extending to ecosystem dynamics, it maps electron flux routing across microbial consortia, showing in which manner nanowire topologies and redox mediators orchestrate dehalogenation pathways amid metabolic competition. Finally, it bridges scales through machine learning-driven multi-omics integration, translating atomic-scale Fe–S coordination patterns into predictive models for optimizing electron flux. Overall, this review provides critical insights for designing next-generation dehalogenation remediation strategies that maximize biotic-abiotic synergies by precisely controlling electron flux.

生物和非生物脱卤策略的整合为修复有机卤化物污染的土壤提供了一种变革性的方法，利用生物选择性和非生物效率的协同效益。然而，在生物-非生物界面调制电子通量分配和重建自适应微生物代谢网络方面的挑战继续阻碍着实际实施。本文综述了生物-非生物协同去除污染场所有机卤化物污染物的最新进展，特别强调了铁硫矿物（FemSn）介导的电子转移机制和微生物代谢网络的调节。在这个框架中，电子通过矿物相表面Fe-S活性位点转移，使电子在与微生物胞外载体和可溶性氧化还原介质的界面上隧穿，从而协调土壤脱氯系统中的通量。本文综述了有机盐呼吸细菌（OHRB）的呼吸电子传递链，同时强调了电子载体利用和能量节约的进化权衡。然后探讨了微生物的相互作用，展示了晶体缺陷工程如何通过电子隧道增强酶的激活并减轻纳米材料的毒性。延伸到生态系统动力学，它绘制了微生物群落的电子通量路径，显示了纳米线拓扑结构和氧化还原介质在代谢竞争中协调脱卤途径的方式。最后，它通过机器学习驱动的多组学集成架起了桥梁，将原子尺度的Fe-S配位模式转化为优化电子通量的预测模型。总的来说，这篇综述为设计下一代脱卤修复策略提供了重要的见解，这些策略可以通过精确控制电子通量来最大化生物-非生物协同作用。

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引用次数: 0