Pub Date : 2022-09-19DOI: 10.38150/sajeb.12(5).p626-638
Wahiba Kara Ali, Zine El Abidine Ababsa, Mounira KARA Ali, Nour Elhouda Ayeb, Asma Ait Kaki, Lilia Douaouya
Tetraclinis articulata is a forest plant species that is widely used in North African folk medicine due to its immense bioactive potential. The objective of the present study was to characterize flavonoids present in butanolic extract of aerial part of T. articulata (TABE) and to test its antibacterial synergistic effect, anticoagulant and antioxidant properties. The analysis of the TABE by colorimetric tests and TLC revealed the presence of flavonoids. The dosage showed that the proportions of total flavonoids and flavonols were 19.25 ± 0.07 and 13.6 ± 1.6 mg QE/g DW, respectively. Besides, the greatest antibacterial activity of the extract was shown against S. aureus. In contrast no effect was observed against E. coli. Furthermore, the most synergistic potential inhibitory effect was noted in the case of E. coli, when the extract was combined with penicillin. In addition, the MICs was determined used microdilution method, the values obtained were ranged from 2.5 to 20 mg/ml. Moreover, the in vitro anticoagulant activity was assessed by PT and KCT tests. The results showed interesting anticoagulant activity, with KCT value of 55.4 s which is superior to that obtained by the anticoagulant drug –Lovenox- (39.37s). The antioxidant activity was evaluated in vitro using DPPH• method. The result expressed in terms of IC50 was found to be 0.04 mg/ml. To conclude, the TABE constitutes a valuable source of anticoagulant, antioxidant and antibacterial synergism metabolites, which makes it a potential candidate to isolate responsible flavonoids and to develop novel pharmaceutical formulations.
{"title":"Phytochemical characterization and biological activities of butanolic extract of aerial parts of Tetraclinis articulata from Algeria","authors":"Wahiba Kara Ali, Zine El Abidine Ababsa, Mounira KARA Ali, Nour Elhouda Ayeb, Asma Ait Kaki, Lilia Douaouya","doi":"10.38150/sajeb.12(5).p626-638","DOIUrl":"https://doi.org/10.38150/sajeb.12(5).p626-638","url":null,"abstract":"Tetraclinis articulata is a forest plant species that is widely used in North African folk medicine due to its immense bioactive potential. The objective of the present study was to characterize flavonoids present in butanolic extract of aerial part of T. articulata (TABE) and to test its antibacterial synergistic effect, anticoagulant and antioxidant properties. The analysis of the TABE by colorimetric tests and TLC revealed the presence of flavonoids. The dosage showed that the proportions of total flavonoids and flavonols were 19.25 ± 0.07 and 13.6 ± 1.6 mg QE/g DW, respectively. Besides, the greatest antibacterial activity of the extract was shown against S. aureus. In contrast no effect was observed against E. coli. Furthermore, the most synergistic potential inhibitory effect was noted in the case of E. coli, when the extract was combined with penicillin. In addition, the MICs was determined used microdilution method, the values obtained were ranged from 2.5 to 20 mg/ml. Moreover, the in vitro anticoagulant activity was assessed by PT and KCT tests. The results showed interesting anticoagulant activity, with KCT value of 55.4 s which is superior to that obtained by the anticoagulant drug –Lovenox- (39.37s). The antioxidant activity was evaluated in vitro using DPPH• method. The result expressed in terms of IC50 was found to be 0.04 mg/ml. To conclude, the TABE constitutes a valuable source of anticoagulant, antioxidant and antibacterial synergism metabolites, which makes it a potential candidate to isolate responsible flavonoids and to develop novel pharmaceutical formulations.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81678825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-19DOI: 10.38150/sajeb.12(5).p585-590
S. Maji, Tejashree Prakash, A. Goyal, A. Tiwari, Razauddin, Maya Ram, R. C. Meena
Utilization of organic substances as fertilizer nutrient sources is now popularising due to over increasing risk of chemical fertilizers used in agriculture. The present field experiment was conducted at Department of Horticulture, Babasaheb Bhimrao Ambedkar University, Lucknow, U.P. (India) to assess the effect of chitosan, an alternative biological origin nutrient source and commercial urea on production of palak. The experiment was comprised of eight treatments namely Control- No fertilizer applied (T0), Recommended Dose of Fertilizer (RDF) @ 90:30:30 N P K kg/ha (T1), Chitosan @ 50 ppm (T2), @ 75 ppm (T3), @ 100 ppm (T4), @ 125 ppm (T5), @ 150 ppm (T6) and Urea @ 1% (T7) and was laid out in Randomized Block Design with three replications. The results showed that foliar application of chitosan and urea significantly influenced the growth, yield and quality of palak. The foliar application of chitosan @ 100 ppm (T4) showed significantly maximum vegetative growth, leaf yield along with better quality of leaves in terms of dry matter content, TSS and Vitamin C content. Urea @ 1% (T7) also showed close result compared to chitosan application. Hence, it will be beneficial to apply chitosan as liquid form @ 100 ppm for spinach beet under subtropical agro-climate condition of Lucknow for better growth, leaf yield and leaf quality.
{"title":"Effect of foliar application of chitosan and urea on growth, yield and quality of palak (Beta vulgaris var. bengalensis L.) cv. All Green","authors":"S. Maji, Tejashree Prakash, A. Goyal, A. Tiwari, Razauddin, Maya Ram, R. C. Meena","doi":"10.38150/sajeb.12(5).p585-590","DOIUrl":"https://doi.org/10.38150/sajeb.12(5).p585-590","url":null,"abstract":"Utilization of organic substances as fertilizer nutrient sources is now popularising due to over increasing risk of chemical fertilizers used in agriculture. The present field experiment was conducted at Department of Horticulture, Babasaheb Bhimrao Ambedkar University, Lucknow, U.P. (India) to assess the effect of chitosan, an alternative biological origin nutrient source and commercial urea on production of palak. The experiment was comprised of eight treatments namely Control- No fertilizer applied (T0), Recommended Dose of Fertilizer (RDF) @ 90:30:30 N P K kg/ha (T1), Chitosan @ 50 ppm (T2), @ 75 ppm (T3), @ 100 ppm (T4), @ 125 ppm (T5), @ 150 ppm (T6) and Urea @ 1% (T7) and was laid out in Randomized Block Design with three replications. The results showed that foliar application of chitosan and urea significantly influenced the growth, yield and quality of palak. The foliar application of chitosan @ 100 ppm (T4) showed significantly maximum vegetative growth, leaf yield along with better quality of leaves in terms of dry matter content, TSS and Vitamin C content. Urea @ 1% (T7) also showed close result compared to chitosan application. Hence, it will be beneficial to apply chitosan as liquid form @ 100 ppm for spinach beet under subtropical agro-climate condition of Lucknow for better growth, leaf yield and leaf quality.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"1988 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90547789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-19DOI: 10.38150/sajeb.12(5).p702-707
L. Rihani
Di-n-butyl phthalate (DBP) is a ubiquitous environmental pollutant, extensively used as a plasticizer in many products including plastics, cosmetics, and medical devices. Some studies have shown that DBP has potential testicular toxicity. The aim of this study was to explore the adverse effects of DBP on testes and epididymis weight, sperm head counts, sperm motility, abnormal changes in sperm morphology, in male rabbits as well as to assess ameliorating role of curcumin. The present study was carried out to evaluate the protective effects of curcumin (from the rhizome, Curcuma longa L.) on the di-n-butylphthalate (DBP)-induced damage in reproductive rabbits. The dose of curcumin (60 mg/kg) and curcumin(60 mg/kg) plus DBP 700 mg/kg were administered per gavage to male rabbits for 30 consecutive days and reproductive parameters were determined.. The results indicated that DBP administration (700 mg/kg) significantly decreased testosterone level, count and motility of sperms, and testis weight compared to control group. However, administration of curcumin with DBP increased reproductive indices in most of the groups compared to DBP-treated groups. DBP-treated rabbits showed marked degeneration of the seminiferous tubules with necrosis and defoliation of spermatocytes .Our research demonstrated that DBP-induced injuries in spermatological parameters and histological structure of testis were recovered by treatment with curcumin Thus, it seems that curcumin inhibits DBP-induced adverse effects on reproductive parameters. The protective effects of these compounds may be due to their intrinsic antioxidant properties and as such may prove useful in combating phthalate-induced reproductive toxicity.
{"title":"Protective role of Curcumin in Di-n-butyl phthalate-induced damage on reproductive parameters in male Rabbits","authors":"L. Rihani","doi":"10.38150/sajeb.12(5).p702-707","DOIUrl":"https://doi.org/10.38150/sajeb.12(5).p702-707","url":null,"abstract":"Di-n-butyl phthalate (DBP) is a ubiquitous environmental pollutant, extensively used as a plasticizer in many products including plastics, cosmetics, and medical devices. Some studies have shown that DBP has potential testicular toxicity. The aim of this study was to explore the adverse effects of DBP on testes and epididymis weight, sperm head counts, sperm motility, abnormal changes in sperm morphology, in male rabbits as well as to assess ameliorating role of curcumin. The present study was carried out to evaluate the protective effects of curcumin (from the rhizome, Curcuma longa L.) on the di-n-butylphthalate (DBP)-induced damage in reproductive rabbits. The dose of curcumin (60 mg/kg) and curcumin(60 mg/kg) plus DBP 700 mg/kg were administered per gavage to male rabbits for 30 consecutive days and reproductive parameters were determined.. The results indicated that DBP administration (700 mg/kg) significantly decreased testosterone level, count and motility of sperms, and testis weight compared to control group. However, administration of curcumin with DBP increased reproductive indices in most of the groups compared to DBP-treated groups. DBP-treated rabbits showed marked degeneration of the seminiferous tubules with necrosis and defoliation of spermatocytes .Our research demonstrated that DBP-induced injuries in spermatological parameters and histological structure of testis were recovered by treatment with curcumin Thus, it seems that curcumin inhibits DBP-induced adverse effects on reproductive parameters. The protective effects of these compounds may be due to their intrinsic antioxidant properties and as such may prove useful in combating phthalate-induced reproductive toxicity.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88557287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-19DOI: 10.38150/sajeb.12(5).p591-598
M. Jayashankar, R. Alkhafaji
Cinnamon oil is derived from the leaves of the cinnamon tree, the essential oil of the plant is extracted by steam distillation, while its bark is used to make cinnamon spice. The present study was conducted to isolate the most important bioactive compounds from cinnamon oil extracted from South India. The thin-layer chromatography (TLC) combined with high-performance liquid chromatography (HPLC) were used to determine and estimate the isolates obtained. It found that the organic phase's highest retention time was 5.433, and the peak area percentage was 88.8 %. The organic phase sample had a high peak area percentage, indicating that there was a lot of bioactive chemical in it. The broad absorption band at 3270 cm-1 is due to the (-OH) group, 1670 and 1623 cm-1 to the C=C group, 1449 and 1381 cm-1 to the (-C-H) group, and the band at 1120 cm-1 to the (-C-O) group. A pseudo molecular ion peak at m/z 275 for a [M+H] +. This study resulted in finding a peak in the positive mode mass spectra, indicating a molecular weight of 274 and the molecular formula of C15H14O5.1H 13CNMR investigations verified the presence of phenol hydroxide groups, two aromatic ring systems, methylene groups, and a phenoxy carbon in the chemical composition of the compound. Moreover, the composition is quite similar to catechins, and this chemical is separated from the organic phase of cinnamon oil. The potent inhibitory activity of the newly synthesized catechin antagonist against S.aureus and E.coli was found to assess cell death by detection of DNA fragments using agarose gel electrophoresis and SEM analysis.
{"title":"Isolation and Purification of the Anti-Bacterial Compound of Cinnamon Oil Extracted from South India","authors":"M. Jayashankar, R. Alkhafaji","doi":"10.38150/sajeb.12(5).p591-598","DOIUrl":"https://doi.org/10.38150/sajeb.12(5).p591-598","url":null,"abstract":"Cinnamon oil is derived from the leaves of the cinnamon tree, the essential oil of the plant is extracted by steam distillation, while its bark is used to make cinnamon spice. The present study was conducted to isolate the most important bioactive compounds from cinnamon oil extracted from South India. The thin-layer chromatography (TLC) combined with high-performance liquid chromatography (HPLC) were used to determine and estimate the isolates obtained. It found that the organic phase's highest retention time was 5.433, and the peak area percentage was 88.8 %. The organic phase sample had a high peak area percentage, indicating that there was a lot of bioactive chemical in it. The broad absorption band at 3270 cm-1 is due to the (-OH) group, 1670 and 1623 cm-1 to the C=C group, 1449 and 1381 cm-1 to the (-C-H) group, and the band at 1120 cm-1 to the (-C-O) group. A pseudo molecular ion peak at m/z 275 for a [M+H] +. This study resulted in finding a peak in the positive mode mass spectra, indicating a molecular weight of 274 and the molecular formula of C15H14O5.1H 13CNMR investigations verified the presence of phenol hydroxide groups, two aromatic ring systems, methylene groups, and a phenoxy carbon in the chemical composition of the compound. Moreover, the composition is quite similar to catechins, and this chemical is separated from the organic phase of cinnamon oil. The potent inhibitory activity of the newly synthesized catechin antagonist against S.aureus and E.coli was found to assess cell death by detection of DNA fragments using agarose gel electrophoresis and SEM analysis.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85132881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-19DOI: 10.38150/sajeb.12(5).p708-715
Radhouane Benmehaia, Ahmed Simozrag, Samir Zeroual
The literature suggest that date palm cultivars are a promising source of beneficial compounds, of which many cultivars around the world are gaining increasing interest due to their important biological properties. Hence, there is a need to be further investigated in Algerian date palm cultivars. The purpose of this study was to identify the effect of the storage temperature variation on the content of phenolic compounds in dates for some Algerian cultivars (Deglet Nour, Ghars, Mech Degla, Degla Baidha). The fruits of the four cultivars were preserved in varying degrees of temperature (4°C and ambient temperature). To determine the effect of the varying degrees of storage temperature on the content of phenolic compounds, the total phenols, flavonoids and total tannins were measured. The differences were noted for the five studied parameters. We found that there was a storage temperature effect on the pH parameter with a value of 6.17 (±0.04), and low effect on electric conductivity. Determination of total polyphenols in dates shows high degrees in Degla Baidha cultivar stored at 4 °C (574.46 mg EAG/100 g) compared to those of Mech Degla, Ghars and Deglet Nour (564.46, 554.35 and 526.39 mg EAG/100 g respectively), and for the same cultivars stored in high temperature, the total polyphenols content was found lower. The total tannin and flavonoid content show a significant difference for date cultivars, where the best estimated levels of the two components in Degla Baidha cultivar at 4 °C (110.43 mg EAT/100 g and 4.51 mg QE/100 g respectively). The results revealed that there was insignificant effect of variation in storage temperatures on flavonoids and total tannins, but the effect was strong, with respect to total polyphenols. Considerable quantities of these compounds were recorded at a temperature of 4 °C. These findings reveal the richness of these Algerian cultivars in phenolic compounds compared to others.
{"title":"Relation between storage temperature and phenolic compounds in date fruits (Phoenix dactylifera L.)","authors":"Radhouane Benmehaia, Ahmed Simozrag, Samir Zeroual","doi":"10.38150/sajeb.12(5).p708-715","DOIUrl":"https://doi.org/10.38150/sajeb.12(5).p708-715","url":null,"abstract":"The literature suggest that date palm cultivars are a promising source of beneficial compounds, of which many cultivars around the world are gaining increasing interest due to their important biological properties. Hence, there is a need to be further investigated in Algerian date palm cultivars. The purpose of this study was to identify the effect of the storage temperature variation on the content of phenolic compounds in dates for some Algerian cultivars (Deglet Nour, Ghars, Mech Degla, Degla Baidha). The fruits of the four cultivars were preserved in varying degrees of temperature (4°C and ambient temperature). To determine the effect of the varying degrees of storage temperature on the content of phenolic compounds, the total phenols, flavonoids and total tannins were measured. The differences were noted for the five studied parameters. We found that there was a storage temperature effect on the pH parameter with a value of 6.17 (±0.04), and low effect on electric conductivity. Determination of total polyphenols in dates shows high degrees in Degla Baidha cultivar stored at 4 °C (574.46 mg EAG/100 g) compared to those of Mech Degla, Ghars and Deglet Nour (564.46, 554.35 and 526.39 mg EAG/100 g respectively), and for the same cultivars stored in high temperature, the total polyphenols content was found lower. The total tannin and flavonoid content show a significant difference for date cultivars, where the best estimated levels of the two components in Degla Baidha cultivar at 4 °C (110.43 mg EAT/100 g and 4.51 mg QE/100 g respectively). The results revealed that there was insignificant effect of variation in storage temperatures on flavonoids and total tannins, but the effect was strong, with respect to total polyphenols. Considerable quantities of these compounds were recorded at a temperature of 4 °C. These findings reveal the richness of these Algerian cultivars in phenolic compounds compared to others.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"91 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80485145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-19DOI: 10.38150/sajeb.12(5).p716-724
Soumia Attou, Yamina Maizi, A. Belmimoun, Khadidja SIDE LARBI, B. Meddah, Aicha Tir touil
Aristolochia longa has been known by its potentiel to cure various diseases.This study was aimd to estimate the antioxidant activity and tannin content of A.longa leaves and roots. Liquid-liquid fractionation by using three solvants was experimented for extraction of tannin from leaves and roots.Then the fractions were oven-dried and powdered to determine the condensed tannin content and antioxidant activity by acidic vanillin, DPPH radical scavenging activity and ferric reducing antioxidant power (FRAP) methods.The qualitative analysis was releazed by high-performance liquid chromatography coupled to mass spectrometry (HPLC DAD-MS).The highest extraction yield was obtained by roots N-butanol fraction (5.15±0.47%).The maximum tannin content value was noted in roots N-butanol fraction (3.90±0.24 mg QUE/g DM) followed by leaves ethyl acetate fraction (3.03±0.01 mg QUE/g DM).The IC50 values of DPPH radical scavenging activity varied significantly from IC50=0.123±0.009mg/ml to 3.737±0.56 mg/ml. Since, the FRAP values ranged from EC50=0.066±0.002 mg/ml to 4,00±0,2 mg/ml. The highest antioxydant ability was observed in leaves and roots N-butanol fractions .Also, the identification by HPLC analysis showed that the roots contains some tannin compounds than leaves such as vescalagin, Roburin D and 1,3,4-tri- O -galloylquinic acid.Our study confirmed the use of A.longa to prevent and control the oxidative stress.
{"title":"Determination of antioxidant activity and tannin content from Aristolochia longa leaves and roots using liquid-liquid fractionation method","authors":"Soumia Attou, Yamina Maizi, A. Belmimoun, Khadidja SIDE LARBI, B. Meddah, Aicha Tir touil","doi":"10.38150/sajeb.12(5).p716-724","DOIUrl":"https://doi.org/10.38150/sajeb.12(5).p716-724","url":null,"abstract":"Aristolochia longa has been known by its potentiel to cure various diseases.This study was aimd to estimate the antioxidant activity and tannin content of A.longa leaves and roots. Liquid-liquid fractionation by using three solvants was experimented for extraction of tannin from leaves and roots.Then the fractions were oven-dried and powdered to determine the condensed tannin content and antioxidant activity by acidic vanillin, DPPH radical scavenging activity and ferric reducing antioxidant power (FRAP) methods.The qualitative analysis was releazed by high-performance liquid chromatography coupled to mass spectrometry (HPLC DAD-MS).The highest extraction yield was obtained by roots N-butanol fraction (5.15±0.47%).The maximum tannin content value was noted in roots N-butanol fraction (3.90±0.24 mg QUE/g DM) followed by leaves ethyl acetate fraction (3.03±0.01 mg QUE/g DM).The IC50 values of DPPH radical scavenging activity varied significantly from IC50=0.123±0.009mg/ml to 3.737±0.56 mg/ml. Since, the FRAP values ranged from EC50=0.066±0.002 mg/ml to 4,00±0,2 mg/ml. The highest antioxydant ability was observed in leaves and roots N-butanol fractions .Also, the identification by HPLC analysis showed that the roots contains some tannin compounds than leaves such as vescalagin, Roburin D and 1,3,4-tri- O -galloylquinic acid.Our study confirmed the use of A.longa to prevent and control the oxidative stress.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73864251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-19DOI: 10.38150/sajeb.12(5).p609-615
Zouaouia Bettayeb, Fawzia Toumi Benali, Abdelfattah Benyamina, Sara Ouldchikh, Said Mohammed El-Amin, Hanane Salem, Youcef Benaissa Keddar
This work deals with the biological activity of the essential oils of an Algerian plant (Cotula cinerea) which belongs to the Asteraceae family. The study was carried out in the south-west of Algeria. We chose essential oils which are natural substances thanks to their numerous anti-infectious, antioxidant and antibacterial properties, etc. The objective of this work is to study the acute toxicity as well as the evaluation of the anti-inflammatory properties of the essential oils of both organs (leaves and flowers) of Cotula cinerea. As methodological approaches, we used the hydrodistillation technique to obtain the essential oils from leaves and flowers. Thus, the acute toxicity was determined by Lorke's method and the anti-inflammatory activity was evaluated by measuring the plantar oedema of mice induced by carrageenan. The results show that the essential oil extracts of the leaves and flowers are low toxic substances with LD50s of 1131.37 mg/kg and 1264.91 mg/kg respectively. They also show a considerable anti-inflammatory effect at doses not exceeding 300 mg/kg with maximum percentages of 86.16% for leaves and 80.87% for flowers. These results show the efficacy of the essential oils of this plant and their therapeutic richness which can be used in traditional medicine for anti-inflammatory treatment. This study has provided additional knowledge on the biological activity of Cotula cinerea essential oils and has opened perspectives for the research of the active principles contained in this species as well as the application of biotechnology to know the molecular mechanism and valorise this natural wealth.
{"title":"Acute toxicity and anti-inflammatory activity of essential oils of Cotula cinerea from southwest Algeria","authors":"Zouaouia Bettayeb, Fawzia Toumi Benali, Abdelfattah Benyamina, Sara Ouldchikh, Said Mohammed El-Amin, Hanane Salem, Youcef Benaissa Keddar","doi":"10.38150/sajeb.12(5).p609-615","DOIUrl":"https://doi.org/10.38150/sajeb.12(5).p609-615","url":null,"abstract":"This work deals with the biological activity of the essential oils of an Algerian plant (Cotula cinerea) which belongs to the Asteraceae family. The study was carried out in the south-west of Algeria. We chose essential oils which are natural substances thanks to their numerous anti-infectious, antioxidant and antibacterial properties, etc. The objective of this work is to study the acute toxicity as well as the evaluation of the anti-inflammatory properties of the essential oils of both organs (leaves and flowers) of Cotula cinerea. As methodological approaches, we used the hydrodistillation technique to obtain the essential oils from leaves and flowers. Thus, the acute toxicity was determined by Lorke's method and the anti-inflammatory activity was evaluated by measuring the plantar oedema of mice induced by carrageenan. The results show that the essential oil extracts of the leaves and flowers are low toxic substances with LD50s of 1131.37 mg/kg and 1264.91 mg/kg respectively. They also show a considerable anti-inflammatory effect at doses not exceeding 300 mg/kg with maximum percentages of 86.16% for leaves and 80.87% for flowers. These results show the efficacy of the essential oils of this plant and their therapeutic richness which can be used in traditional medicine for anti-inflammatory treatment. This study has provided additional knowledge on the biological activity of Cotula cinerea essential oils and has opened perspectives for the research of the active principles contained in this species as well as the application of biotechnology to know the molecular mechanism and valorise this natural wealth.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82397591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In North Africa, Nerium oleander is quite common vegetation in the steppe zone. In Algeria also, its presence is quite common, especially on alluvial and stony soils. However, data on its phytochemical profiling are scanty. Therefore, the present study was designed to reveal phenolic diversity, identify some major flavonoid molecules, detect and elucidate the biological property of N. oleander leaves. Initially the extraction methods and strategies were optimized using various solvents and various phases were obtained. Subsequently, the phases obtained on various classes of phenolic molecules were confronted with analytical chromatography in the presence of an appropriate reagent and spectral analysis. Overall, five pure and native products namely kaemphferol, quercetin, luteolin, apigenin, quercetin 3OR were separated and purified.
{"title":"The extraction and identification of flavonoids from Nerium oleander L. in the east of Algeria - Constantine","authors":"Madaci Brahim, Kabouche Samy, Merghem Rachid, Derradji Lotfi","doi":"10.38150/sajeb.12(5).p639-644","DOIUrl":"https://doi.org/10.38150/sajeb.12(5).p639-644","url":null,"abstract":"In North Africa, Nerium oleander is quite common vegetation in the steppe zone. In Algeria also, its presence is quite common, especially on alluvial and stony soils. However, data on its phytochemical profiling are scanty. Therefore, the present study was designed to reveal phenolic diversity, identify some major flavonoid molecules, detect and elucidate the biological property of N. oleander leaves. Initially the extraction methods and strategies were optimized using various solvents and various phases were obtained. Subsequently, the phases obtained on various classes of phenolic molecules were confronted with analytical chromatography in the presence of an appropriate reagent and spectral analysis. Overall, five pure and native products namely kaemphferol, quercetin, luteolin, apigenin, quercetin 3OR were separated and purified.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86943086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-19DOI: 10.38150/sajeb.12(5).p671-676
Ouarda Semmame, Khadija Sedrati, H. Ziada, N. Abadi, Dalila Sata
Angiotensin-converting enzyme (ACE) has been reported to be associated with the pathogenesis and progression of human cancers. Nevertheless, the details of that role remain inconclusive. We conducted this case-control study to determine the frequency of the ACE I/D polymorphism and its relationship to the risk of cervical cancer in the Algerian population. Genotype analysis of the ACE I/D polymorphism was performed using polymerase chain reaction (PCR) in DNA isolated from peripheral blood samples of 88 cervical cancer patients and 161 healthy controls subjects. Overall, the frequencies of ID, II, and DD genotypes showed a significant difference between cervical cancer patients and controls (p <0.0001). The I allele frequencies were more frequent in cervical cancer patients than in healthy subjects. Contrary to expectation, individuals’ carriers of the I allele (ID +II) had a significant increase in cervical cancer risk when using the genotype DD as the reference category (II+ID vs. DD: OR, 6; 95% CI, 2.71-13.29, p <0.0001). Our results indicate that ACE I/D polymorphism may contribute to the development of cervical oncogenesis and might be used as a common risk factor for cervical cancer susceptibility.
血管紧张素转换酶(ACE)已被报道与人类癌症的发病和进展有关。然而，这一作用的细节仍然没有定论。我们进行了这项病例对照研究，以确定阿尔及利亚人群中ACE I/D多态性的频率及其与宫颈癌风险的关系。应用聚合酶链反应(PCR)对88例宫颈癌患者和161例健康对照者外周血DNA进行了ACE I/D多态性基因型分析。总体而言，宫颈癌患者与对照组的ID、II和DD基因型频率差异有统计学意义(p <0.0001)。宫颈癌患者的I等位基因频率高于健康人群。与预期相反，当使用基因型DD作为参考类别时，携带I等位基因(ID +II)的个体患宫颈癌的风险显著增加(II+ID vs. DD: OR, 6;95% CI, 2.71 ~ 13.29, p <0.0001)。我们的研究结果表明，ACE I/D多态性可能有助于宫颈癌的发生发展，并可能作为宫颈癌易感性的共同危险因素。
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Diabetes is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. The chronic hyperglycemia of diabetes is associated with long-term damage, dysfunction, and failure of various organs. Accordingly, in the current study we have investigated the effect of clove on lipid metabolism, and on oxidative stress-induced inflammation response in diabetic rats. Diabetes was induced intraperitonially by a single injection of streptozotocin (STZ). The treated group received daily orally clove extract, for 4 weeks. Daily treatment by clove lowered significantly blood glucose, HbA1c levels and ameliorates the lipid profiles and showed increased PON1 activity. The study of lipid peroxidation showed that, the treatment with clove decreased TBARS levels in LDL fraction and in pancreas. Furthermore, in pancreas TNF-alpha and IL-1β levels were decreased in treated group. These results were confirmed by the histopathological assessments which clove treatment showed marked improvement of the destructive effect on pancreas islet cells induced by STZ. The present findings clearly demonstrated that clove could prevent many diabetic complications by reducing inflammation and oxidative damage. Thus, the effectiveness of clove in treating diabetes potentially may possibly due to presence of bioactive compounds.
{"title":"Clove (Syzygium aromaticum) ameliorates oxidative stress-induced inflammation and improves reverse cholesterol transport and paraoxonase 1 activity in diabetic rats","authors":"Akila Guenzet, Sadia Berzou, Mostefa Khelladi, Hakima Mir, Nawal Dida, D. Krouf","doi":"10.38150/sajeb.12(5).p616-625","DOIUrl":"https://doi.org/10.38150/sajeb.12(5).p616-625","url":null,"abstract":"Diabetes is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. The chronic hyperglycemia of diabetes is associated with long-term damage, dysfunction, and failure of various organs. Accordingly, in the current study we have investigated the effect of clove on lipid metabolism, and on oxidative stress-induced inflammation response in diabetic rats. Diabetes was induced intraperitonially by a single injection of streptozotocin (STZ). The treated group received daily orally clove extract, for 4 weeks. Daily treatment by clove lowered significantly blood glucose, HbA1c levels and ameliorates the lipid profiles and showed increased PON1 activity. The study of lipid peroxidation showed that, the treatment with clove decreased TBARS levels in LDL fraction and in pancreas. Furthermore, in pancreas TNF-alpha and IL-1β levels were decreased in treated group. These results were confirmed by the histopathological assessments which clove treatment showed marked improvement of the destructive effect on pancreas islet cells induced by STZ. The present findings clearly demonstrated that clove could prevent many diabetic complications by reducing inflammation and oxidative damage. Thus, the effectiveness of clove in treating diabetes potentially may possibly due to presence of bioactive compounds.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73925746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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