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Novel cinnamic acid derivatives enable the induction of chloroplast-associated immunity and interaction with viral coat proteins for plant viral disease control. 新的肉桂酸衍生物能够诱导叶绿体相关免疫并与病毒外壳蛋白相互作用,用于植物病毒疾病控制。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-07 DOI: 10.1002/ps.70703
Rong-Shuang Luo,Xiang-Xu Zhou,Guo Wang,Yan-Wei Huang,Wu-Bin Shao,Dan Zeng,Hao-Yu Zhang,Hong-Mei Xiang,Xiang Zhou,Yu-Cheng Gu,Song Yang
BACKGROUNDViral diseases remain persistent global challenges to public health systems and agricultural productivity, while current antiviral strategies are constrained by their narrow therapeutic spectrum and overreliance on unimodal mechanisms. These critical limitations highlight the imperative to develop new antiviral agents with innovative chemical scaffolds and multimodal mechanisms of action.RESULTSIn this study, 31 novel cinnamic acid derivatives were synthesized and their inhibitory activities against tobacco mosaic virus (TMV) in tobacco hosts were evaluated. Compound B7, which features a p-bromophenyl group connected via a piperidine linker and a sulfonamide pharmacophore, demonstrated the most potent antiviral efficacy, with half maximal effective concentration (EC50) values of 224.75 mg L-1 (protective) and 332.59 mg L-1 (curative), outperforming the positive control ningnanmycin (245.67 and 340.52 mg L-1, respectively). Mechanistic investigations revealed that B7 significantly reduced viral accumulation in chloroplast-associated regions, thereby enhancing plant vitality and promoting chloroplast-mediated immune regulation. Meanwhile, B7 can also increase the salicylic acid content in plants. These effects ultimately lead to activated plant resistance. Furthermore, B7 also exhibited strong binding affinity (Ka = 1.19 × 105 m-1) to TMV coat protein (TMV-CP), directly disrupting viral structural integrity and inhibiting replication.CONCLUSIONThis study identifies B7 as the optimal antiviral agent with superior efficacy and biosafety, defines critical structure-activity relationship principles governing cinnamic acid-based antiviral agent design, and mechanistically validates its dual-action mechanisms involving chloroplast-potentiated systemic immunity and direct TMV-CP interactions. It provides an important reference for the development of efficient and multitarget antiviral agents. © 2026 Society of Chemical Industry.
病毒性疾病仍然是全球公共卫生系统和农业生产力面临的持续挑战,而目前的抗病毒策略受到治疗范围狭窄和过度依赖单峰机制的限制。这些关键的限制突出了开发具有创新化学支架和多模态作用机制的新型抗病毒药物的必要性。结果合成了31个新的肉桂酸衍生物,并测定了它们对烟草花叶病毒(TMV)的抑制活性。化合物B7的最大有效浓度(EC50)分别为224.75 mg L-1(保护性)和332.59 mg L-1(治疗性),优于阳性对照宁南霉素(245.67和340.52 mg L-1),其对溴苯基通过哌啶连接体和磺胺药效团连接。机制研究表明,B7可显著减少叶绿体相关区域的病毒积累,从而增强植物活力,促进叶绿体介导的免疫调节。同时,B7还能提高植物体内水杨酸的含量。这些影响最终导致植物的抗性被激活。B7对TMV外壳蛋白(TMV- cp)具有较强的结合亲和力(Ka = 1.19 × 105 m-1),直接破坏病毒结构完整性,抑制病毒复制。结论本研究确定了B7为最佳的抗病毒药物,具有优越的生物安全性和有效性,确定了肉桂酸类抗病毒药物设计的关键构效关系原则,并从机制上验证了其叶绿体增强全身免疫和TMV-CP直接相互作用的双重作用机制。为开发高效多靶点抗病毒药物提供了重要参考。©2026化学工业协会。
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引用次数: 0
Development of 1,2,4-oxadiazole-containing isoxazoline insecticides with improved honeybee compatibility. 改善蜜蜂相容性的1,2,4-恶二唑类异恶唑啉类杀虫剂的研制。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-06 DOI: 10.1002/ps.70708
Qiu Liu,Xingjie Zhang,Shang Wu,Ziming Wang,Yuqin Luo,Wenchao Wei,Deyu Hu,Runjiang Song
BACKGROUNDSpodoptera frugiperda (S. frugiperda) and Plutella xylostella (P. xylostella) are major constraints to staple and cruciferous crops, respectively, and have evolved widespread resistance to anthranilic amide insecticides. Isoxazolines, acting as γ-aminobutyric acid (GABA) receptor modulators, are promising alternatives for controlling resistant populations. However, many members of this class exhibit high toxicity toward ecologically important pollinators. Here, we report a series of new isoxazoline analogues featuring a 1,2,4-oxadiazole motif designed by a ring-closure strategy to the fluxametamide scaffold, and we systematically assess their insecticidal efficacy, honeybee toxicity, and interaction with receptors.RESULTSIncorporation of a 1,2,4-oxadiazole moiety enabled the design, synthesis, and bioevaluation of 34 novel derivatives. Compound L1 displayed potent activity against P. xylostella, with a median lethal concentration (LC50) of 0.89 mg L-1. After chiral resolution, S-(+)-L1 showed markedly enhanced potency (LC50 = 0.69 mg L-1), being 159.36-fold more active than R-(-)-L1 (LC50 = 109.96 mg L-1) and comparable to fluxametamide (LC50 = 0.51 mg L-1). Notably, S-(+)-L1 exhibited substantially reduced acute contact toxicity to honeybees (LD50 = 4.58 μg a.i./bee) relative to fluxametamide (LD50 = 0.25 μg a.i./bee). Enzyme-linked immunosorbent assay (ELISA)-based assays and molecular docking suggested that S-(+)-L1 binds more favorably to the pest GABA receptor than its enantiomer, while the 1,2,4-oxadiazole unit specifically weakens hydrogen-bond interactions with the bee GABA receptor, thereby mitigating toxicity.CONCLUSIONS-(+)-L1 is identified as a selective lead combining high pesticidal potency with improved pollinator safety, suggesting ring-closure-enabled scaffold hopping as a viable strategy to retain on-target engagement while enhancing non-target compatibility. © 2026 Society of Chemical Industry.
背景:果蛾spodoptera frugiperda (S. frugiperda)和小菜蛾(P. xylostella)分别是主食作物和十字花科作物的主要害虫,它们已经进化出对邻苯二甲酸类杀虫剂的广泛抗性。异恶唑啉作为γ-氨基丁酸(GABA)受体调节剂,是控制耐药种群的有希望的替代品。然而,这一类的许多成员对生态上重要的传粉媒介表现出高毒性。在这里,我们报道了一系列新的异恶唑啉类似物,这些类似物具有1,2,4-恶二唑基序,通过环闭合策略设计到氟沙美胺支架上,我们系统地评估了它们的杀虫功效、蜜蜂毒性以及与受体的相互作用。结果1,2,4-恶二唑片段的合成使34个新型衍生物的设计、合成和生物评价成为可能。化合物L1对小菜蛾有较强的杀伤活性,中位致死浓度为0.89 mg L-1。经手性拆分后,S-(+)- l1的效价明显增强(LC50 = 0.69 mg L-1),比R-(-)- l1 (LC50 = 109.96 mg L-1)的效价高159.36倍,与氟沙美胺(LC50 = 0.51 mg L-1)相当。值得注意的是,S-(+)- l1对蜜蜂的急性接触毒性(LD50 = 4.58 μg a.i./蜜蜂)明显低于氟沙美胺(LD50 = 0.25 μg a.i./蜜蜂)。基于酶联免疫吸附试验(ELISA)的分析和分子对接表明,S-(+)- l1与害虫GABA受体的结合比其对映体更有利,而1,2,4-恶二唑单元特异性地减弱了与蜜蜂GABA受体的氢键相互作用,从而减轻了毒性。结论:-(+)- l1是一种选择性先导物,结合了高杀虫效力和提高传粉者的安全性,表明环闭合支架跳跃是一种可行的策略,可以在保持靶标接合的同时增强非靶标相容性。©2026化学工业协会。
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引用次数: 0
Sublethal effects of insecticides on the predatory stink bug Arma custos and its prey, the tea geometrid Ectropis grisescens: prospects for integrated pest management in tea plantations. 杀虫剂对捕食性臭蝽及其猎物茶尺蠖的亚致死效应:茶园害虫综合治理的展望。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-06 DOI: 10.1002/ps.70698
Qi Liu,Yang-Fan Kuang,Hao-Xiang Chen,Chang-Jin Lin,Li-Jun Cai,Geoff M Gurr,Liette Vasseur,Chen-Xi Liu,Yu-Xin Xu,Li-Lin Chen
BACKGROUNDInsecticide applications can have adverse effects on not only target pests, but also beneficial arthropods. In this study, we examined such effects on a key tea pest, Ectropis grisescens, and its natural enemy, Arma custos. Laboratory toxicity bioassays were conducted and we evaluated the toxicity profiles toward each insect species of three commonly used insecticides, bifenthrin, chlorfenapyr, and emamectin benzoate.RESULTSAt maximum field-recommended concentrations, all three insecticides were unsafe for first to fourth instars of A. custos. Bifenthrin and emamectin benzoate also showed considerable toxicity to the fifth instar, whereas chlorfenapyr exhibited relative safety. Selectivity toxicity ratios indicated that all the tested insecticides were less toxic to the predator than to the pest. After exposure to sublethal concentrations (LC20), the functional response models of fifth-instar A. custos were still consistently Holling II in all groups. However, insecticide exposure significantly prolonged handling time (Th) when predators fed on second- and third-instar E. grisescens, and reduced their predation capacity (a/Th) and maximum daily predation (Na-max). By contrast, when preying on fourth-instar E. grisescens, exposure to insecticides increased the instantaneous attack rate (a) and a/Th of A. custos. Notably, chlorfenapyr shortened Th and enhanced both a/Th and Na-max on this prey instar.CONCLUSIONThis study highlights the potential of integrating A. custos with selective insecticides for the sustainable management of E. grisescens in tea plantations. To maximize pest control efficacy, we recommend releasing A. custos during early stages of E. grisescens infestation, particularly following the application of insecticides exhibiting favorable selectivity profiles. © 2026 Society of Chemical Industry.
农药的使用不仅会对目标害虫产生不利影响,也会对有益的节肢动物产生不利影响。在这项研究中,我们研究了对一种主要的茶叶害虫——灰背黄萎病及其天敌——褐背黄萎病的影响。进行了实验室毒性生物测定,评估了3种常用杀虫剂联苯菊酯、氯虫腈和甲维菌素对每种昆虫的毒性特征。结果3种杀虫剂对1 ~ 4龄家蝇均不安全。联苯菊酯和甲维菌素苯甲酸酯对5龄幼虫也有相当大的毒性,而氯虫腈对5龄幼虫具有相对的安全性。选择性毒性比表明,所有被试杀虫剂对捕食者的毒性小于对害虫的毒性。亚致死浓度(LC20)后,各组5龄斑蝽的功能反应模型仍一致为Holling II型。然而,杀虫剂暴露显著延长了捕食二龄和三龄稻蠹蛾的处理时间(Th),降低了其捕食能力(a/Th)和最大日捕食量(Na-max)。与此相反,当捕食四龄灰斑姬蝇时,暴露于杀虫剂会增加灰斑姬蝇的瞬时攻击率(a)和a/Th。值得注意的是,虫螨腈缩短了该捕食星的Th,提高了a/Th和Na-max。结论本研究为茶园褐飞虱的可持续管理提供了选择性杀虫剂与褐飞虱的结合。为了最大限度地提高害虫防治效果,我们建议在灰稻瘟病发生的早期阶段,特别是在使用了具有良好选择性的杀虫剂之后,释放小仓鼠。©2026化学工业协会。
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引用次数: 0
The gene encoding chitin deacetylase is a potential target for RNAi-based control of Laodelphax striatellus Fallén (Hemiptera: Delphacidae). 编码几丁质去乙酰化酶的基因是基于rnai控制斑纹蚜(半翅目:斑纹蚜科)的潜在目标。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-06 DOI: 10.1002/ps.70640
Wenxing Hu,Ao You,Yang Cao,Zhipeng Huang,Lu Zhang,Fang Liu
BACKGROUNDChitin deacetylase (CDA) is a key enzyme in chitin modification. However, its functions in the agricultural pest Laodelphax striatellus (small brown planthopper, SBPH), particularly those relevant to pest control, remain largely unexplored.RESULTSThe CDAs encoded by LsCDA1, LsCDA2 and LsCDA4 were highly expressed in fifth-instar nymphs and eggs. Injection of double-stranded RNAs (dsRNAs) targeting LsCDA1 and LsCDA2 (designated dsLsCDA1 and dsLsCDA2) induced over 95% mortality in nymphs and complete lethality (100%) in eggs. Silencing LsCDA1 and LsCDA2 suppressed the expression of chitin synthesis- and degradation-related enzymes, while simultaneously inhibited the degradation of old cuticle and the formation of new cuticle in nymphs, as well as reduced chitosan content and cuticle integrity in eggs. These findings reveal a novel role for dsLsCDA1 and dsLsCDA2 in the egg lethality of hemimetabolous insects. The cross-species ovicidal efficacy of dsLsCDA1 and dsLsCDA2 was further validated in another notorious rice pest, Nilaparvata lugens (brown planthopper, BPH), which extended beyond the known nymphal lethality of dsRNA targets NlCDA1 and NlCDA2 in BPH. Based on these results, oral delivery of a bacterially transcribed dsRNA-CDA chimera targeting LsCDA1 and NlCDA1 significantly suppressed CDA1 expression in both species, leading to 43% and 49% nymph mortality in SBPH and BPH, and 53% and 41% egg lethality in SBPH and BPH, respectively.CONCLUSIONThese results reveal the novel physiological functions of CDA in molting and egg development, thereby highlighting its potential as a target for RNA interference-mediated control of SBPH. © 2026 Society of Chemical Industry.
几丁质脱乙酰酶(CDA)是几丁质修饰的关键酶。然而,其对农业害虫褐飞虱(小褐飞虱,SBPH)的作用,特别是与害虫防治有关的作用,在很大程度上仍未被探索。结果LsCDA1、LsCDA2和LsCDA4编码的cdna在五龄若虫和卵中高表达。注射靶向LsCDA1和LsCDA2(指定为dsLsCDA1和dsLsCDA2)的双链rna (dsRNAs)可诱导若虫95%以上的死亡率和卵100%的完全致死率。沉默LsCDA1和LsCDA2抑制了几丁质合成和降解相关酶的表达,同时抑制了若虫旧角质层的降解和新角质层的形成,降低了壳聚糖含量和卵角质层的完整性。这些发现揭示了dsLsCDA1和dsLsCDA2在半代谢昆虫卵致死性中的新作用。dsLsCDA1和dsLsCDA2对褐飞虱(Nilaparvata lugens, BPH)的杀卵效果进一步得到验证,其杀卵效果超出了已知的dsRNA靶点NlCDA1和NlCDA2在褐飞虱中的雌雄致死性。基于这些结果,口服靶向LsCDA1和NlCDA1的细菌转录dsRNA-CDA嵌合体可显著抑制两种物种中CDA1的表达,导致SBPH和BPH的若虫死亡率分别为43%和49%,SBPH和BPH的卵死亡率分别为53%和41%。结论这些结果揭示了CDA在脱毛和卵发育中的新生理功能,从而突出了其作为RNA干扰介导的SBPH控制靶点的潜力。©2026化学工业协会。
{"title":"The gene encoding chitin deacetylase is a potential target for RNAi-based control of Laodelphax striatellus Fallén (Hemiptera: Delphacidae).","authors":"Wenxing Hu,Ao You,Yang Cao,Zhipeng Huang,Lu Zhang,Fang Liu","doi":"10.1002/ps.70640","DOIUrl":"https://doi.org/10.1002/ps.70640","url":null,"abstract":"BACKGROUNDChitin deacetylase (CDA) is a key enzyme in chitin modification. However, its functions in the agricultural pest Laodelphax striatellus (small brown planthopper, SBPH), particularly those relevant to pest control, remain largely unexplored.RESULTSThe CDAs encoded by LsCDA1, LsCDA2 and LsCDA4 were highly expressed in fifth-instar nymphs and eggs. Injection of double-stranded RNAs (dsRNAs) targeting LsCDA1 and LsCDA2 (designated dsLsCDA1 and dsLsCDA2) induced over 95% mortality in nymphs and complete lethality (100%) in eggs. Silencing LsCDA1 and LsCDA2 suppressed the expression of chitin synthesis- and degradation-related enzymes, while simultaneously inhibited the degradation of old cuticle and the formation of new cuticle in nymphs, as well as reduced chitosan content and cuticle integrity in eggs. These findings reveal a novel role for dsLsCDA1 and dsLsCDA2 in the egg lethality of hemimetabolous insects. The cross-species ovicidal efficacy of dsLsCDA1 and dsLsCDA2 was further validated in another notorious rice pest, Nilaparvata lugens (brown planthopper, BPH), which extended beyond the known nymphal lethality of dsRNA targets NlCDA1 and NlCDA2 in BPH. Based on these results, oral delivery of a bacterially transcribed dsRNA-CDA chimera targeting LsCDA1 and NlCDA1 significantly suppressed CDA1 expression in both species, leading to 43% and 49% nymph mortality in SBPH and BPH, and 53% and 41% egg lethality in SBPH and BPH, respectively.CONCLUSIONThese results reveal the novel physiological functions of CDA in molting and egg development, thereby highlighting its potential as a target for RNA interference-mediated control of SBPH. © 2026 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"52 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147368458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mark-release-recapture study of irradiated male Aedes albopictus under stressful field conditions. 野外应激条件下辐照雄性白纹伊蚊的标记释放-再捕获研究。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-06 DOI: 10.1002/ps.70706
Qingdeng Feng,Ming Li,Jeremy Bouyer,Jehangir Khan,Zhifeng Ling,Yiming Cai,Xufei Zhao,Marco Carrieri,Yu Wu,Xiaoying Zheng,Zhongdao Wu,Dongjing Zhang
BACKGROUNDThe sterile insect technique (SIT) suppresses mosquito populations by releasing sterile males. Its success mainly depends on the performance of sterile males. Mark-Release-Recapture (MRR) experiments are used to evaluate male performance in the field, yet most previous experiments were conducted under mild conditions (e.g. 25-28 °C) in which mosquitoes could easily adapt. Male mosquito performance under stressful field conditions remains poorly understood. This study evaluated the field performance of irradiated Aedes albopictus males under high-temperature conditions (>30 °C in average) in southern China, Foshan City, located in a sub-tropical area. Two MRR experiments were conducted in a 5-ha village during July-August of 2023 and 2024. Lifespan and dispersal of postreleased males were examined using BG-Sentinel mosquito traps, human landing catches, and ovitraps.RESULTSA total of 54 546 and 58 440 sterile males were released in 2023 and 2024, respectively, with recapture rates of 8.53% and 5.64%. The mean dispersal distances ranged from 58.88-77.19 m (2023) to 81.82-90.86 m (2024). Daily survival probabilities were 0.15-0.28 in 2023 and 0.45-0.62 in 2024, corresponding to average expected lifespans of 0.53-0.78 days and 1.26-2.13 days, respectively. Estimated wild male densities ranged from 302-986 males ha-1 in 2023 to 416-3785 males ha-1 in 2024.CONCLUSIONStressful field conditions severely reduced sterile male survival and dispersal, highlighting the need to optimize release protocols in challenging climates. These findings provide critical baseline data for advancing Ae. albopictus SIT programmes in southern China. © 2026 Society of Chemical Industry.
昆虫不育技术(SIT)通过释放不育雄蚊来抑制蚊子数量。它的成功主要取决于不育雄性的表现。标记-释放-再捕获(MRR)实验用于评估雄蚊在野外的表现,但之前的大多数实验都是在温和条件下(例如25-28°C)进行的,蚊子很容易适应。雄蚊在野外应激条件下的表现仍然知之甚少。本研究以地处亚热带地区的佛山市为研究对象,对高温条件下辐照白纹伊蚊雄蚊的田间表现进行了评价。在2023年7 - 8月和2024年8月在一个5 ha的村庄进行了两次MRR实验。利用BG-Sentinel捕蚊器、人工落网捕蚊器和诱卵器对释放后雄蚊的寿命和扩散情况进行了研究。结果2023年和2024年共放归不育雄蚊54 546只和58 440只,再抓率分别为8.53%和5.64%。平均扩散距离为58.88 ~ 77.19 m(2023年)~ 81.82 ~ 90.86 m(2024年)。2023年的每日生存概率为0.15-0.28,2024年为0.45-0.62,对应的平均预期寿命分别为0.53-0.78天和1.26-2.13天。估计野生雄性密度在2023年为302-986雄ha-1, 2024年为416-3785雄ha-1。结论恶劣的野外环境严重降低了不育雄虫的生存和扩散,强调了在恶劣气候条件下优化释放方案的必要性。这些发现为推进Ae提供了关键的基线数据。中国南方白纹伊蚊SIT项目。©2026化学工业协会。
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引用次数: 0
Microbe applications to soil lead to prey-mediated effects on predatory mite with potential for a detrimental biocontrol outcome. 微生物应用于土壤导致捕食螨的介导效应与潜在的有害的生物防治结果。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-06 DOI: 10.1002/ps.70709
Hong Yan,Endong Wang,Guo-Shu Wei,Xuenong Xu,Ary A Hoffmann,Mark R McNeill,Bo Zhang
BACKGROUNDBeneficial microbes are increasingly applied as biocontrol agents to enhance soil vitality and crop productivity. However, given that alterations in soil conditions can propagate across trophic levels, it is essential to evaluate the broader ecological effects and biosafety of soil-applied microorganisms on higher trophic organisms. This study investigates the impact of two beneficial soil-applied microbes, Bacillus cereus and Chryseobacterium cucumeris, on the soil-borne plant pathogenic fungus Fusarium oxysporum, cucumber, the two-spotted spider mite Tetranychus urticae and its predator Phytoseiulus persimilis.RESULTSBoth bacterial agents were effective against F. oxysporum and demonstrated the ability to be transmitted across plant-herbivore-predator trophic levels. Notably, B. cereus was vertically transmitted from adults to eggs in P. persimilis, whereas C. cucumeris was only detected in the digestive tract. Bacillus cereus had no significant effects on fitness of both mite species. In contrast, C. cucumeris significantly reduced survival and reproduction of T. urticae and survival of P. persimilis adults, but not egg production. Greenhouse trials confirmed that soil application of C. cucumeris around the roots of cucumber, and subsequent uptake in the foliage could significantly suppress the survival and reproduction of both mite species.CONCLUSIONThis study demonstrates that soil-introduced biocontrol microbes can transmit across trophic levels and may exert non-target effects on predators. The findings underscore the importance of considering indirect ecological risks and biosafety implications for beneficial invertebrates when deploying microbial biocontrol agents in integrated pest management systems. © 2026 Society of Chemical Industry.
有益微生物作为生物防治剂被越来越多地应用于提高土壤活力和作物生产力。然而,考虑到土壤条件的改变可以跨营养水平传播,有必要评估土壤施用微生物对高营养生物的更广泛的生态效应和生物安全性。本研究探讨了蜡样芽孢杆菌和黄瓜黄杆菌两种有益土壤微生物对土传植物病原真菌尖孢镰刀菌、黄瓜、荨麻疹叶螨及其捕食者perseulus persimilis的影响。结果两种细菌制剂均对尖孢镰刀菌有效,并具有跨植物-草食-捕食营养水平传播的能力。值得注意的是,蜡样芽孢杆菌在persimilis中由成虫垂直传播到卵,而黄瓜芽孢杆菌仅在消化道中检测到。蜡样芽孢杆菌对两种螨的适合度均无显著影响。相比之下,c . cucumeris显著降低生存和繁殖的t . p . persimilis成人的测定和生存,而不是鸡蛋生产。温室试验证实,黄瓜根周土壤施用和叶片吸收可显著抑制两种螨的生存和繁殖。结论本研究表明,土壤引入的生物防治微生物可以跨营养水平传播,并可能对捕食者产生非靶效应。研究结果强调了在综合虫害管理系统中部署微生物生物防治剂时考虑间接生态风险和对有益无脊椎动物的生物安全影响的重要性。©2026化学工业协会。
{"title":"Microbe applications to soil lead to prey-mediated effects on predatory mite with potential for a detrimental biocontrol outcome.","authors":"Hong Yan,Endong Wang,Guo-Shu Wei,Xuenong Xu,Ary A Hoffmann,Mark R McNeill,Bo Zhang","doi":"10.1002/ps.70709","DOIUrl":"https://doi.org/10.1002/ps.70709","url":null,"abstract":"BACKGROUNDBeneficial microbes are increasingly applied as biocontrol agents to enhance soil vitality and crop productivity. However, given that alterations in soil conditions can propagate across trophic levels, it is essential to evaluate the broader ecological effects and biosafety of soil-applied microorganisms on higher trophic organisms. This study investigates the impact of two beneficial soil-applied microbes, Bacillus cereus and Chryseobacterium cucumeris, on the soil-borne plant pathogenic fungus Fusarium oxysporum, cucumber, the two-spotted spider mite Tetranychus urticae and its predator Phytoseiulus persimilis.RESULTSBoth bacterial agents were effective against F. oxysporum and demonstrated the ability to be transmitted across plant-herbivore-predator trophic levels. Notably, B. cereus was vertically transmitted from adults to eggs in P. persimilis, whereas C. cucumeris was only detected in the digestive tract. Bacillus cereus had no significant effects on fitness of both mite species. In contrast, C. cucumeris significantly reduced survival and reproduction of T. urticae and survival of P. persimilis adults, but not egg production. Greenhouse trials confirmed that soil application of C. cucumeris around the roots of cucumber, and subsequent uptake in the foliage could significantly suppress the survival and reproduction of both mite species.CONCLUSIONThis study demonstrates that soil-introduced biocontrol microbes can transmit across trophic levels and may exert non-target effects on predators. The findings underscore the importance of considering indirect ecological risks and biosafety implications for beneficial invertebrates when deploying microbial biocontrol agents in integrated pest management systems. © 2026 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"14 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147368461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect and safety evaluation of MIL-101 loaded with prochloraz against rice sheath blight. 载药MIL-101防治水稻纹枯病的效果及安全性评价。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-06 DOI: 10.1002/ps.70659
Bingjie Pan,Hongchen Liu,Yifei Li,Xiaotong Feng,Shujing Yu,Anyu Gu,Kun Qian
BACKGROUNDRice sheath blight (ShB), a fungal disease caused by Rhizoctonia solani, seriously threatens rice yield and quality. Prochloraz (PRO), a broad-spectrum imidazole fungicide, may cause some harm to the environment and organisms when overused. To improve its efficiency and reduce environmental impact, MIL-101, a metal-organic framework, was chosen as carrier to load prochloraz to make PRO@MIL-101.RESULTSCharacterizations including high-performance liquid chromatography (HPLC), thermogravimetric analysis (TGA) and X-ray diffraction (XRD) confirmed successful drug loading, with MIL-101 maintaining a regular octahedral structure and good stability. In vitro release studies showed that PRO@MIL-101 exhibited a rapid release behavior, with a faster release under acidic conditions, and fitted the Ritger-Peppas model. PRO@MIL-101 also showed better wetting properties in contact angle and adhesion function assays. Antifungal tests indicated that PRO@MIL-101 achieved strong inhibitory effects at relatively low concentrations. Safety evaluations demonstrated that MIL-101 promoted rice growth and chlorophyll synthesis at low-to-medium concentrations, which may enhance rice disease resistance by increasing antioxidant enzyme activities.CONCLUSIONThe study successfully constructs a prochloraz delivery system based on MIL-101. The system has a drug-loading rate of ≈10%, and exhibits excellent drug release performance in an acidic environment and strong adhesion to leaf surfaces. MIL-101 is safe for rice, as it can promote rice growth and enhance stress resistance. Overall, these results suggest that the drug-loading system shows an excellent control effect on ShB, providing a new idea for reducing pesticide usage while improving efficacy of its control effect. © 2026 Society of Chemical Industry.
水稻纹枯病(ShB)是由枯丝核菌引起的一种真菌病害,严重威胁水稻产量和品质。丙氯唑(Prochloraz,简称PRO)是一种广谱咪唑类杀菌剂,过量使用会对环境和生物造成一定危害。为了提高效率,减少对环境的影响,选择金属-有机骨架MIL-101作为载药载体,通过高效液相色谱(HPLC)、热重分析(TGA)和x射线衍射(XRD)验证了PRO@MIL-101.RESULTSCharacterizations载药成功,MIL-101保持了规则的八面体结构和良好的稳定性。体外释放研究表明PRO@MIL-101具有快速释放行为,在酸性条件下释放速度更快,符合Ritger-Peppas模型。PRO@MIL-101在接触角和粘附功能测试中也表现出较好的润湿性能。抗真菌试验表明PRO@MIL-101在较低浓度下具有较强的抑制作用。安全性评价表明,MIL-101在中低浓度下促进水稻生长和叶绿素合成,可能通过提高抗氧化酶活性来增强水稻抗病性。结论本研究成功构建了一种基于MIL-101的咪氯嗪给药体系。该体系载药率约为10%,在酸性环境下具有优异的释药性能,且与叶片表面的附着力强。MIL-101对水稻是安全的,因为它能促进水稻生长,增强抗逆性。综上所述,该载药系统对ShB具有良好的防治效果,为减少农药用量,提高防治效果提供了新的思路。©2026化学工业协会。
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引用次数: 0
Comparison of air and ground insecticide coverage and duration of control for navel orangeworm (Amyelois transitella Walker) (Lepidoptera: Pyralidae) in California tree nuts. 加利福尼亚树坚果中脐橙虫(鳞翅目:皮蚜科)空气与地面杀虫剂覆盖及防治时间的比较。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-06 DOI: 10.1002/ps.70705
Joel P Siegel
BACKGROUNDInsecticide application is necessary to control navel orangeworm (Amyelois transitella) in California tree nuts. Coverage is problematic because of height, and application timing can be problematic due to logistical challenges combined with grower reaction time. Bioassays using contact toxicity were conducted to characterize both spray coverage and the duration of control for air application for a period of 14-28 days, and contrast air application with ground sprays.RESULTSGround application caused higher mortality than air application at a critical height of 3.7-4.3 m, and its duration of control was superior, based on higher mortality at 21 days. In one study, the mortality resulting from ground application remained constant at 89% for 21 days, whereas the average mortality for air application fell to 62%, although this was still satisfactory. There was a rapid decline in contact toxicity between 4 and 7 days after application by air. The mortality observed with air application did not depend on the water volume used because there was no difference between 93.5 L ha-1 and 280.6 L ha-1.CONCLUSIONThe focus of this study on contact toxicity is the first step toward establishing a baseline for this route of exposure in tree nuts, and additional baselines are needed for adulticidal and ovi-larvicidal activity. Air application was capable of providing high initial mortality but its duration needs to be extended. Improving coverage and application timing will become more important as California phases out the use of synthetic insecticides and makes the transition to insecticides solely dependent on ingestion toxicity. Published 2026. This article is a U.S. Government work and is in the public domain in the USA.
背景在加利福尼亚树坚果中应用杀虫剂防治脐橙虫是必要的。由于高度的原因,覆盖范围存在问题,并且由于后勤挑战和种植者的反应时间,应用时机可能存在问题。使用接触毒性进行生物测定,以表征喷雾覆盖范围和空气施用的控制时间,为期14-28天,并将空气施用与地面施用进行对比。结果在临界高度为3.7 ~ 4.3 m时,地面施药死亡率高于空中施药,且施药时间以21 d死亡率较高为优势。在一项研究中,地面施用造成的死亡率在21天内保持在89%不变,而空中施用的平均死亡率下降到62%,尽管这仍然令人满意。空气施药后4 ~ 7天,接触毒性迅速下降。使用空气时观察到的死亡率与使用的水量无关,因为93.5 L ha-1和280.6 L ha-1之间没有差异。结论本研究的重点是接触毒性,这是建立这一接触途径的基线的第一步,还需要进一步研究杀成虫和杀卵活性的基线。空中施用能够提供高的初始死亡率,但其持续时间需要延长。随着加州逐步停止使用合成杀虫剂,转而使用完全取决于摄入毒性的杀虫剂,改善覆盖范围和施用时间将变得更加重要。2026年出版。这篇文章是美国政府的作品,在美国属于公有领域。
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引用次数: 0
Residue dynamics and dietary risks of diflufenican and flufenacet in wheat using field experiments and model simulations. 利用田间试验和模型模拟,双氟螨尼和氟螨塞在小麦中的残留动态和膳食风险。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-05 DOI: 10.1002/ps.70702
Nannan Pang,Xinze Liu,Peter Fantke,Qi Zhang,Liyuan Liu,Qiaozhen Chen,Qiyu Gong,Jiye Hu
BACKGROUNDPesticide usage and related food safety and human health concerns have received great attention worldwide. The phenyl ether and oxyacetamid herbicides diflufenican and flufenacet are widely used in wheat production in China and beyond to control grasses and broad-leaved weeds. We hence assessed their residue behaviors in wheat production and evaluated related dietary risks for humans.RESULTSComparing field trials and an established method of QuEChERS (quick, easy, cheap, effective, rugged and safe) liquid chromatography tandem mass spectrometry (LC-MS/MS) with simulations using a dynamic plant uptake model gave consistent residue results in wheat for both diflufenican and flufenacet. Residue levels were consistently below their respective maximum residue limits in China. Dissipation half-lives for diflufenican were in the range 7.4-16 days in straw and 11-43 days in soil, whereas dissipation half-lives for flufenacet were on average somewhat shorter, at 1.7-3.3 days in straw and 9.8-38 days in soil. Combining residues with human intake estimates allows for assessing related dietary exposure and risk. Chronic and acute risk quotients for both pesticides indicated acceptable dietary risk for consuming wheat products with respect to using diflufenican and flufenacet at recommended doses.CONCLUSIONCombining field trials with simulations from a dynamic plant uptake model allows for an effective and efficient evaluation of pesticide residues and estimates of related dietary risks for humans. Our approach can be extended to assess additional pesticides, crops and regions to foster safer pesticide use practices. © 2026 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
农药使用及其相关的食品安全和人类健康问题在世界范围内受到广泛关注。苯醚类除草剂和氧乙酰胺类除草剂双氟芬尼肯和氟芬塞在国内外小麦生产中广泛用于控制禾草和阔叶杂草。因此,我们评估了它们在小麦生产中的残留行为,并评估了相关的人类饮食风险。结果将田间试验和建立的QuEChERS(快速、简便、廉价、有效、可靠、安全)液相色谱串联质谱(LC-MS/MS)方法与动态植物吸收模型模拟结果进行比较,得到了双氟虫尼和氟虫那塞在小麦中的残留结果一致。中国的残留水平一直低于各自的最大残留限量。异氟螨尼在秸秆中的耗散半衰期为7.4 ~ 16天,在土壤中的耗散半衰期为11 ~ 43天,而氟螨塞在秸秆中的耗散半衰期平均略短,为1.7 ~ 3.3天,在土壤中的耗散半衰期为9.8 ~ 38天。将残留物与人体摄入估计相结合,可以评估相关的饮食暴露和风险。这两种农药的慢性和急性风险商数表明,在推荐剂量下使用双氟芬尼和氟芬塞,食用小麦产品的饮食风险是可以接受的。结论将田间试验与动态植物吸收模型模拟相结合,可以有效和高效地评估农药残留并估计人类相关的饮食风险。我们的方法可以扩展到评估更多的农药、作物和地区,以促进更安全的农药使用做法。©2026作者。由John Wiley & Sons Ltd代表化学工业协会出版的《害虫管理科学》。
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引用次数: 0
Biological and molecular mechanisms of dual resistance to fludioxonil and tebuconazole in a Fusarium graminearum field isolate from China. 中国禾谷镰刀菌田间分离株对氟菌腈和戊唑唑双重抗性的生物学和分子机制。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-05 DOI: 10.1002/ps.70683
Chenguang Wang,Lin Yang,Ruiping Huang,Chengxiang Dai,Weiwei Cong,Zhili Yuan,Yiping Hou
BACKGROUNDFusarium head blight (FHB) caused by Fusarium graminearum severely threatens wheat production. Although fludioxonil and tebuconazole are key fungicides for FHB control, emerging resistance has reduced their efficacy, and no dual-resistant F. graminearum strains have yet been reported.RESULTSWe collected 120 F. graminearum isolates in 2024 from Shandong, Shanxi, Hebei, and Anhui provinces. Among them, 119 isolates showed sensitivities to fludioxonil ranging from 0.0084 to 0.0392 μg/mL, with an average EC50 (effective concentration 50%) value of 0.0238 ± 0.0059 μg/mL. We identified one isolate exhibiting dual resistance to both fludioxonil and tebuconazole. Its ultrahigh fludioxonil resistance was mediated by a novel point mutation, FgOs1A307V. Site-directed mutagenesis confirmed that FgOs1A307V conferred ultrahigh fludioxonil resistance (EC50 >100, RF (resistance factor) >4000). Tebuconazole resistance is attributed to FgCyp51B overexpression, conferring low-level resistance (20 >RF >5). Furthermore, the FgOs1A307V mutation caused defects in asexual and sexual reproduction and reduced pathogenicity. The mutant also exhibited osmotic stress hypersensitivity and reduced glycerol accumulation. The dual-resistant isolate showed no cross-resistance to carbendazim, phenamacril, pydiflumetofen, or pyraclostrobin.CONCLUSIONThis study demonstrates that F. graminearum strains with dual resistance to fludioxonil and tebuconazole have emerged in the field. It clarifies that fludioxonil resistance and tebuconazole resistance are conferred by FgOs1A307V mutation and FgCyp51B overexpression, respectively. The dual-resistant strain exhibited a significant fitness cost. These findings inform sustainable fungicide rotation and resistance management for more effective FHB control. © 2026 Society of Chemical Industry.
背景小麦赤霉病(Fusarium head blight, FHB)是由小麦赤霉病引起的一种严重威胁小麦生产的病害。虽然氟恶菌腈和戊康唑是控制FHB的关键杀菌剂,但新出现的耐药性已经降低了它们的效果,而且目前还没有双耐药的F. graminearum菌株的报道。结果采集到120个F。2024年山东、山西、河北和安徽等省的禾本科分离株。其中,119株菌株对氟菌腈的敏感范围为0.0084 ~ 0.0392 μg/mL,平均EC50(有效浓度50%)为0.0238±0.0059 μg/mL。我们鉴定出一株对氟菌腈和戊康唑均有双重耐药性的分离株。其超高的抗疫能力是由一个新的点突变FgOs1A307V介导的。定点诱变证实FgOs1A307V具有超高的流菌腈抗性(EC50 bb100, RF(抗性因子)bb1000)。戊康唑耐药归因于FgCyp51B过表达,赋予低水平耐药(20 >RF >5)。此外,FgOs1A307V突变导致无性生殖和有性生殖缺陷,并降低致病性。突变体还表现出渗透应激超敏反应和甘油积累减少。双耐药分离株对多菌灵、非那马克利、吡氟甲醚或吡咯菌酯无交叉耐药。结论本研究表明,田间已出现对氟恶菌腈和戊康唑具有双重抗性的禾本科禾耳镰刀菌。结果表明,氟恶菌腈耐药和戊康唑耐药分别由FgOs1A307V突变和FgCyp51B过表达引起。双抗菌株表现出显著的适应度成本。这些发现为可持续的杀菌剂轮作和耐药性管理提供了信息,以便更有效地控制食毒菌。©2026化学工业协会。
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引用次数: 0
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Pest Management Science
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