Pest Management Science最新文献

The commercialization of 2,4-D (2,4-dichlorophenoxyacetic acid) latifolicide in 1945 marked the beginning of the selective herbicide market, with this active ingredient playing a pivotal role among commercial herbicides due to the natural tolerance of monocots compared with dicots. Due to its intricate mode of action, involving interactions within endogenous auxin signaling networks, 2,4-D was initially considered a low-risk herbicide to evolve weed resistance. However, the intensification of 2,4-D use has contributed to the emergence of 2,4-D-resistant broadleaf weeds, challenging earlier beliefs. This review explores 2,4-D tolerance in crops and evolved resistance in weeds, emphasizing an in-depth understanding of 2,4-D metabolic detoxification. Nine confirmed 2,4-D-resistant weed species, driven by rapid metabolism, highlight cytochrome P450 monooxygenases in Phase I and glycosyltransferases in Phase II as key enzymes. Resistance to 2,4-D may also involve impaired translocation associated with mutations in auxin/indole-3-acetic acid (Aux/IAA) co-receptor genes. Moreover, temperature variations affect 2,4-D efficacy, with high temperatures increasing herbicide metabolism rates and reducing weed control, while drought stress did not affect 2,4-D efficacy. Research on 2,4-D resistance has primarily focused on non-target-site resistance (NTSR) mechanisms, including 2,4-D metabolic detoxification, with limited exploration of the inheritance and genetic basis underlying these traits. Resistance to 2,4-D in weeds is typically governed by a single gene, either dominant or incompletely dominant, raising questions about gain-of-function or loss-of-function mutations that confer resistance. Future research should unravel the physiological and molecular-genetic basis of 2,4-D NTSR, exploring potential cross-resistance patterns and assessing fitness costs that may affect future evolution of auxin-resistant weeds. © 2024 Society of Chemical Industry.

Background: The Vip3Aa insecticidal protein, produced by Bacillus thuringiensis, has been effectively used in commercial Bt-crops to manage lepidopteran pests. Upon ingestion by larvae, the protoxin is processed by midgut proteases into the activated protein and binds specifically to its receptors in the midgut, leading to insect mortality. Cryo-EM resolution of the trypsin-processed Vip3Aa protein unveiled structural remodelling of the N-terminal region during the transition from protoxin to activated protein. This conformational change has been demonstrated to be crucial for toxicity against Spodoptera exigua larvae, a major global lepidopteran pest. In this study, we investigated the relevance of the structural remodelling for the specific binding to midgut receptors.

Results: We conducted in vitro binding assays with radiolabelled proteins and brush border membrane vesicles (BBMV) from S. exigua, employing structural mutants that lock the protein in either its protoxin or its activated conformation. Our results indicate that both structural stages of the protein share binding sites in the midgut epithelium. Moreover, in vivo competition assays revealed that Vip3Aa is able to bind to functional receptors in S. exigua larvae both as protoxin and as activated protein.

Conclusion: Altogether, our findings point to both structural conformations contributing to receptor binding. In vivo, either spontaneous structural shift upon proteolytic cleavage or receptor-mediated remodelling could be occurring. However, the timing and context in which the conformational change occurs could influence membrane insertion and toxicity. Our results show the complex interplay between proteolytic processing, protein structure and receptor interactions in Vip3Aa's toxicity. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Background: Migration and immunity are behavioral and physiological traits that protect organisms from environmental stressors or pathogen infection. Shifting from migration to residency has become more common in some wildlife populations owing to environmental changes. However, other biological shifts, such as interactions between migration and immunity among populations within a species are largely unexplored for many agricultural migratory pests. In the field, entomopathogenic fungi infection and transmission, particularly Beauveria bassiana, can cause reduced fitness and population declines across a broad range of insect species.

Results: Here, we investigated migration-immunity interactions between migrant and resident populations of the oriental armyworm, Mythimna separata, infected with B. bassiana (the sole fungus used in this work). We found that migratory M. separata exerted stronger pathogen resistance, faster development and lower pupal weight than residents. High-dose infections (5.0 × 10⁵ and 5.0 × 10⁶ conidia mL^-1) led to seriously decreased reproductive capacity in migrants and residents. Low-dose infections (1.0 × 10⁴ and 5.0 × 10⁴ conidia mL^-1) led to significantly increased host flight capacities. Consecutive flight tests showed that five flight nights inhibited the reproduction of paternal infected M. separata populations. The flights also led to far-reaching transgenerational impairment of larval development and immune defense among offspring populations. By contrast, two flight nights enhanced the reproductive capacities of both M. separata populations and did not exert negative transgenerational effects on offspring populations, which may facilitate migration.

Conclusions: This study provides insights into interactions between migration and immunity among M. separata populations. These insights will guide development of future monitoring and management technologies of this pest. © 2024 Society of Chemical Industry.

The cover image is based on the article RNA interference as a next-generation control method for suppressing Varroa destructor reproduction in honey bee (Apis mellifera) hives by Rose A. McGruddy et al., https://doi.org/10.1002/ps.8193.

Background: Crops are consistently under siege by a multitude of pathogens. These pathogenic microorganisms, including viruses and bacteria, result in substantial reductions in quality and yield globally by inducing detrimental crop diseases, thus posing a significant challenge to global food security. However, the biological activity sepectrum of commercially available pesticides is limited and the pesticide efficacy is poor, necessitating the urgent development of broad-spectrum and efficient strategies for crop disease prevention and control.

Results: The bioassay results revealed that certain target compounds demonstrated outstanding in vivo antiviral efficacy against cucumber mosaic virus and tobacco mosaic virus. In particular, compound D6 showed remarkable antiviral activity against CMV, significantly higher than that of the control agent ningnanmycin. Mechanism of action studies have shown that compound D6 could enhance the activity of defense enzymes and upregulate the expression of genes related to disease resistance, thereby enhancing the antiviral effects in plants. In addition, these compounds displayed superior inhibitory activity against plant bacterial diseases. For Xoo, compound D10 showed an excellent inhibitory effect that was better than that of the control agent bismerthiazol. Scanning electron microscopy and fluorescence double-staining experiments revealed that compound D10 effectively inhibited bacterial growth by disrupting the cell membrane.

Conclusion: A series of trifluoromethyl hydrazone derivatives were designed and synthesized, and it was found that they have control effects on plant viruses and bacterial diseases. In addition, this study revealed the mechanism of action of the active compounds and demonstrated their potential as multifunctional crop protectants. © 2024 Society of Chemical Industry.

Background: Integrated pest management (IPM) is recognized as a sustainable approach to pest control. However, adoption rates among farmers in Iran have been observed to be low. This study investigates the adoption of IPM practices among citrus farmers in Iran and assesses the applicability of the protection motivation theory (PMT) in explaining farmers' behavior towards IPM.

Results: Overall, farmers reported poor levels of IPM use, paying attention only to monitoring practices among the three groups of IPM practices examined (prevention, monitoring, and suppression). The perceived severity of the consequences of chemical pest control (β = 0.389, P < 0.001), the response efficacy of IPM practices (β = 0.254, P < 0.001), and the perceived self-efficacy of using IPM practices (β = 0.199, P < 0.001) positively predicted adoption of IPM practices, while response cost/barriers of IPM practices (β = -0.355, P < 0.001) negatively predicted adoption of IPM practices.

Conclusion: The PMT model presents a unique and interesting perspective to understand if and how IPM practices against the threat of pests are motivated. The perceived severity of the consequences of chemical pest control showed the strongest positive effect, whereas the response cost/barriers of IPM practices showed the strongest negative effect on IPM adoption. © 2024 Society of Chemical Industry.

Background: Owing to large amounts of synthetic pesticides being extensively and unreasonably used for crop protection, currently, resistance and negative impacts on human health and environment safety have appeared. Therefore, development of potential pesticide candidates is highly urgent. Herein, a series of ester derivatives of osthole were designed and synthesized as pesticidal agents.

Results: Six spatial configurations of 4'-(p-toluenoyloxy)osthole (4b), 4'-(m-fluorobenzoyloxy)osthole (4f), 4'-(p-fluorophenylacetyloxy)osthole (4m), 4'-(3'',4''-methylenedioxybenzoyloxy)osthole (4q), 4'-formyloxyosthole (4u) and 4'-acetyloxyosthole (4v) were determined by X-ray mono-crystal diffraction. Compounds 4b, 4'-(p-chlorobenzoyloxy)osthole (4g), 4'-(m-chlorobenzoyloxy)osthole (4h), 4'-(p-bromobenzoyloxy)osthole (4i) and 4'-(2''-chloropyridin-3''-ylcarbonyloxy)osthole (4p) showed higher insecticidal activity than toosendanin against Mythimna separata Walker; notably, compound 4b displayed 1.8 times insecticidal activity of the precursor osthole. Against Tetranychus cinnabarinus Boisduval, compounds 4g and 4h showed 3.3 and 2.6 times acaricidal activity of osthole, and good control effects in the glasshouse. Scanning electron microscopy assay demonstrated that compound 4g can damage the cuticle layer of T. cinnabarinus resulting in death.

Conclusion: Compounds 4g and 4h can be further studied as lead pesticidal agents for the management of M. separata and T. cinnabarinus. These results will pave the way for application of osthole derivatives as agrochemicals. © 2024 Society of Chemical Industry.

Background: Poplar in China has long been plagued by the fall webworm Hyphantria cunea. Enhancing plant immunity using chemical elicitors is an environmentally friendly approach to pest control. The phytohormone methyl jasmonate (MeJA) can stimulate the chemical defenses of poplars against herbivores but has been shown to have limited efficacy in practice. Here, we studied the effects of a MeJA and mesoporous silica nanoparticle (MSN) complex (MeJA@MSN) regarding the induction of poplar resistance to H. cunea, which may provide strategies for the effective use of MeJA.

Results: The silicon-based phytohormone complex (MeJA@MSNs) exhibited excellent biological and physiochemical properties, such as excellent biocompatibility and plant tissue transportability. The changes in metabolites in poplar leaves induced by MeJA, MSNs, and MeJA@MSNs were investigated by metabolic analysis. MeJA@MSNs led to highly potent induced resistance along with elevated salicylaldehyde content, which increased with the dose administered. The salicylaldehyde metabolite showed a strong antifeedant effect on H. cunea larvae at a dosage of 1 μg, with the 50% lethal dose being 20.4 μg/mg. Furthermore, transcriptional analysis showed that MeJA@MSNs upregulated key genes in biosynthetic pathways more than MeJA and MSNs.

Conclusion: Our results show that MeJA and MSNs interact positively in poplar, leading to salicylaldehyde accumulation and increased induced resistance to H. cunea, providing new insights into the underlying resistance mechanisms induced by MeJA@MSNs. © 2024 Society of Chemical Industry.

Background: While pesticides are essential for crop protection and food security, they pose serious risks to human health and the environment. Agro-input dealers can play an important role in mitigating pesticide risks, given that they are a major source of pesticides and plant health information for many developing-country farmers. In this article, we assess the willingness of agro-input dealers to offer integrated pest management-based advisory services and promote pesticide risk reduction through a voluntary certification scheme.

Results: Using survey data from 557 agro-input dealers in Uganda and a discrete choice experiment, we find that the proposed certification scheme is significantly valued by agro-input dealers, particularly for its potential to provide training opportunities and ensure safety to human health and the environment. Agro-input dealers have a positive attitude towards a certification scheme that restricts the sale of high-risk pesticide products, especially if it stimulates additional income-generating opportunities. Further analysis shows that preferences for voluntary certification attributes are influenced by certification experience, agro-dealership experience, business ownership status and incidence of acute pesticide poisoning.

Conclusion: The study findings demonstrate that agro-input dealers are conscious of pesticide risks to human and environmental health and are keen to participate in a certification scheme promoting safer plant protection products. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Background: The pine wood nematode Bursaphelenchus xylophilus, a severe invasive species, is responsible for causing widespread pine wilt disease. The CytCo protein, a pore-forming toxin derived from Conidiobolus obscurus, exhibits nematotoxicity towards B. xylophilus.

Results: Our present study reveals the expression variation of a range of gene products in B. xylophilus that respond to the effects of CytCo using the isobaric tags for relative and absolute quantification proteomics technology. Functional enrichment analysis indicates that many differentially expressed proteins are linked to calcium signaling system, proteasome, energy production and conversion, and the determination of adult lifespan. It suggests that the dysregulation of calcium homeostasis, energy metabolism, and apoptosis contribute to the CytCo nematotoxicity. Using the calcium ion (Ca²⁺)-indicator calcein, we detected changes in Ca²⁺ levels in B. xylophilus, with a significantly increase in fluorescence in the nematode's intestine and pseudocoelom following CytCo treatments. Meanwhile, the apoptosis and reactive oxygen species (ROS) assays showed an enhancement of fluorescence in B. xylophilus cells, with increased CytCo concentrations.

Conclusion: The protein toxin CytCo triggers Ca²⁺ leakage, disrupts Ca²⁺ balance in B. xylophilus, and induces apoptosis and ROS outburst, thereby intensifying its nematotoxic effects. This finding facilitates our understanding of the modes of action of nematotoxic proteins, and contributes to the development of innovative nematode control strategies. © 2024 Society of Chemical Industry.