首页 > 最新文献

Pest Management Science最新文献

英文 中文
Molecular co-assembly engineering towards natural tea saponin-based nanopesticides for synergistic enhanced foliar affinity. 基于天然茶皂素的纳米农药的分子共组装工程,协同增强叶面亲和性。
IF 3.8 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-04-01 Epub Date: 2025-12-30 DOI: 10.1002/ps.70504
Enguang Ma, Jun Ma, Ning Ma, Hui Li, Yekai Song, Longlong Geng, Hui Hu, Zhinan Fu, Jinwen Qin, Xinfang Wang, Baoyong Zhu, Xuhong Guo

Background: Emerging as transformative agricultural nanotechnologies, nanopesticides have brought unique opportunities for optimal pesticide efficacy because of their inherent properties. However, existing nanopesticides are often plagued by costly and toxic carrier materials, as well as limitations in preparation techniques.

Results: Here, we report a facile and scalable flash nanoprecipitation (FNP) approach, based on molecular co-assembly engineering of natural tea saponin (TS) and tannic acid (TA), to synthesize abamectin (Abm) nanopesticides (Abm@TS/TA nanopesticides). The developed FNP approach circumvented the limitations of batch processing by enabling controllable and continuous production of Abm@TS/TA nanopesticides with a total flow rate of 8640 mL h-1. More importantly, co-assembly of natural carriers with Abm has been harnessed to achieve synergistic nanopesticides of uniform spherical structure, excellent stability, favorable sustained-release properties, and comparable biological efficacy against Tetranychus urticae. Moreover, the synergistic interfacial properties of the engineered nanopesticides demonstrate improved leaf affinity and retention on plant surfaces compared with conventional formulations.

Conclusion: This work establishes an expandable and efficacious approach for deploying eco-friendly nanopesticide systems for sustainable agricultural applications. © 2025 Society of Chemical Industry.

背景:纳米农药作为一种革命性的农业纳米技术,由于其固有的特性,为优化农药药效带来了独特的机会。然而,现有的纳米杀虫剂经常受到昂贵和有毒的载体材料的困扰,以及制备技术的限制。结果:本文报道了一种基于天然茶皂素(TS)和单宁酸(TA)分子共组装工程的快速快速纳米沉淀(FNP)方法合成阿维菌素(Abm)纳米农药(Abm@TS/TA纳米农药)。开发的FNP方法绕过了批量处理的限制,实现了Abm@TS/TA纳米农药的可控和连续生产,总流量为8640 mL h-1。更重要的是,利用天然载体与Abm的共组装,获得了具有均匀球形结构、优异稳定性、良好缓释性能和对荨麻疹叶螨具有相当生物功效的协同纳米农药。此外,与传统配方相比,工程纳米农药的协同界面特性显示出更好的叶片亲和性和在植物表面的保留率。结论:这项工作为可持续农业应用部署生态友好型纳米农药系统建立了一种可扩展和有效的方法。©2025化学工业协会。
{"title":"Molecular co-assembly engineering towards natural tea saponin-based nanopesticides for synergistic enhanced foliar affinity.","authors":"Enguang Ma, Jun Ma, Ning Ma, Hui Li, Yekai Song, Longlong Geng, Hui Hu, Zhinan Fu, Jinwen Qin, Xinfang Wang, Baoyong Zhu, Xuhong Guo","doi":"10.1002/ps.70504","DOIUrl":"10.1002/ps.70504","url":null,"abstract":"<p><strong>Background: </strong>Emerging as transformative agricultural nanotechnologies, nanopesticides have brought unique opportunities for optimal pesticide efficacy because of their inherent properties. However, existing nanopesticides are often plagued by costly and toxic carrier materials, as well as limitations in preparation techniques.</p><p><strong>Results: </strong>Here, we report a facile and scalable flash nanoprecipitation (FNP) approach, based on molecular co-assembly engineering of natural tea saponin (TS) and tannic acid (TA), to synthesize abamectin (Abm) nanopesticides (Abm@TS/TA nanopesticides). The developed FNP approach circumvented the limitations of batch processing by enabling controllable and continuous production of Abm@TS/TA nanopesticides with a total flow rate of 8640 mL h<sup>-1</sup>. More importantly, co-assembly of natural carriers with Abm has been harnessed to achieve synergistic nanopesticides of uniform spherical structure, excellent stability, favorable sustained-release properties, and comparable biological efficacy against Tetranychus urticae. Moreover, the synergistic interfacial properties of the engineered nanopesticides demonstrate improved leaf affinity and retention on plant surfaces compared with conventional formulations.</p><p><strong>Conclusion: </strong>This work establishes an expandable and efficacious approach for deploying eco-friendly nanopesticide systems for sustainable agricultural applications. © 2025 Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":"3907-3914"},"PeriodicalIF":3.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145861742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular biology of insect ATPases: from physiology to pest management. 昆虫三磷酸腺苷酶的分子生物学:从生理学到害虫防治。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-23 DOI: 10.1002/ps.70711
Azam Amiri,Ali R Bandani
ATPases are indispensable molecular engines that couple ATP hydrolysis to ion transport and mechanical work, enabling pH regulation, ion homeostasis, neuronal excitability, and cellular energy production across insects. Three major families of ATPases (vacuolar-type H+-ATPases (V-ATPases), P-type ATPases, and F-type ATP synthases) exhibit distinct architectures, tissue localizations, and physiological roles, ranging from epithelial energization and calcium signaling to mitochondrial ATP synthesis. Recent structural and genomic studies have revealed extensive isoform diversity and lineage-specific adaptations, underscoring the central role of isoforms in insect physiology. Considering this functional framework, we appraise the growing evidence that these same ATPases provide strategic entry points for RNA interference (RNAi)-based pest management. Using RNAi of V-ATPase subunits consistently yields lethal or sterilizing phenotypes. In contrast, emerging data suggest that P- and F-type ATPases are involved in reproduction, osmoregulation, and energy metabolism, and are of scientific merit for RNAi. Advances in double-stranded RNA (dsRNA) design, nanoparticle carriers, and plant-, microbe-, or spray-based delivery systems expand the practical reach of this approach, although species-specific barriers and ecological safeguards remain critical. Integrating physiological insights along with applied perspectives, this review highlights insect ATPases as both fundamental drivers of life processes and promising molecular targets. Such dual emphasis provides a roadmap for exploiting ATPase diversity while preserving ecological balance in next-generation pest-management strategies. © 2026 Society of Chemical Industry.
ATP酶是不可或缺的分子引擎,将ATP水解与离子运输和机械工作结合起来,实现昆虫的pH调节、离子稳态、神经元兴奋性和细胞能量产生。三种主要的ATP酶家族(液泡型H+-ATP酶(v -ATP酶)、p型ATP酶和f型ATP合成酶)表现出不同的结构、组织定位和生理作用,从上皮激活、钙信号传导到线粒体ATP合成。最近的结构和基因组研究揭示了广泛的同种异构体多样性和谱系特异性适应,强调了同种异构体在昆虫生理学中的核心作用。考虑到这一功能框架,我们评估了越来越多的证据表明,这些相同的atp酶为基于RNA干扰(RNAi)的害虫管理提供了战略切入点。使用v - atp酶亚基的RNAi始终产生致死或不育表型。相反,新出现的数据表明P型和f型atp酶参与生殖、渗透调节和能量代谢,对RNAi具有科学价值。双链RNA (dsRNA)设计、纳米颗粒载体和基于植物、微生物或喷雾的递送系统的进展扩大了这种方法的实际应用范围,尽管物种特异性屏障和生态保障仍然至关重要。结合生理学的见解和应用的观点,本文强调了昆虫atp酶既是生命过程的基本驱动因素,也是有前途的分子靶点。这种双重强调为下一代害虫管理策略提供了利用atp酶多样性同时保持生态平衡的路线图。©2026化学工业协会。
{"title":"Molecular biology of insect ATPases: from physiology to pest management.","authors":"Azam Amiri,Ali R Bandani","doi":"10.1002/ps.70711","DOIUrl":"https://doi.org/10.1002/ps.70711","url":null,"abstract":"ATPases are indispensable molecular engines that couple ATP hydrolysis to ion transport and mechanical work, enabling pH regulation, ion homeostasis, neuronal excitability, and cellular energy production across insects. Three major families of ATPases (vacuolar-type H+-ATPases (V-ATPases), P-type ATPases, and F-type ATP synthases) exhibit distinct architectures, tissue localizations, and physiological roles, ranging from epithelial energization and calcium signaling to mitochondrial ATP synthesis. Recent structural and genomic studies have revealed extensive isoform diversity and lineage-specific adaptations, underscoring the central role of isoforms in insect physiology. Considering this functional framework, we appraise the growing evidence that these same ATPases provide strategic entry points for RNA interference (RNAi)-based pest management. Using RNAi of V-ATPase subunits consistently yields lethal or sterilizing phenotypes. In contrast, emerging data suggest that P- and F-type ATPases are involved in reproduction, osmoregulation, and energy metabolism, and are of scientific merit for RNAi. Advances in double-stranded RNA (dsRNA) design, nanoparticle carriers, and plant-, microbe-, or spray-based delivery systems expand the practical reach of this approach, although species-specific barriers and ecological safeguards remain critical. Integrating physiological insights along with applied perspectives, this review highlights insect ATPases as both fundamental drivers of life processes and promising molecular targets. Such dual emphasis provides a roadmap for exploiting ATPase diversity while preserving ecological balance in next-generation pest-management strategies. © 2026 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"45 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147502355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Potential patterns of fall armyworm seasonal migration in West Africa modeled with atmospheric trajectory analyses. 用大气轨迹分析模拟西非秋粘虫季节性迁徙的潜在模式。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-22 DOI: 10.1002/ps.70732
Fan-Qi Gao,Xue-Yan Zhang,Rosina Kyerematen,Gao Hu,Regan Early,Jason W Chapman
BACKGROUNDThe migratory invasive species fall armyworm (Spodoptera frugiperda, FAW) has established year-round populations in several West African countries following its initial invasion of Africa in early 2016. However, its seasonal migratory dynamics within West Africa remain poorly understood. If FAW populations in West Africa were able to successfully cross the Sahara Desert and serve as a major source population in North Africa, this could increase the risk of further invasion into southern Europe. In this study, we used atmospheric data to perform trajectory simulations, predicting the seasonal migratory pathways of short-distance migratory FAW individuals within West African breeding habitats and assessing the monthly probabilities of long-distance migrants departing from West Africa successfully crossing the Sahara Desert.RESULTSThe results indicate that from May to September, the vast majority of short-distance migrants (>70%) remained within West African breeding habitats, whereas in other months, a larger proportion of individuals were blown into the Atlantic Ocean. Moreover, short-distance migrants exhibited clear seasonal movement patterns within West Africa: shifting southwestward from January to May, turning northeastward in June and July, and returning southwestward from August to December. Long-distance migrants had an extremely low success rate (≤0.3%) of crossing the Sahara Desert, which occurred only between February and April each year.CONCLUSIONThis study revealed the seasonal migration patterns of FAW within West Africa, providing important insights for predicting regional outbreak risks and optimizing management strategies in the region. © 2026 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
迁徙入侵物种落粘虫(Spodoptera frugiperda, FAW)自2016年初首次入侵非洲以来,已在几个西非国家建立了全年种群。然而,其在西非的季节性迁徙动态仍然知之甚少。如果西非的一汽种群能够成功地穿越撒哈拉沙漠,成为北非的主要来源种群,这可能会增加进一步入侵南欧的风险。在这项研究中,我们使用大气数据进行轨迹模拟,预测了西非繁殖栖息地短距离迁徙的FAW个体的季节性迁徙路径,并评估了每月从西非出发的长途迁徙者成功穿越撒哈拉沙漠的概率。结果5 - 9月,绝大多数短距离迁徙者(约70%)停留在西非的繁殖地内,而在其他月份,更大比例的个体被吹入大西洋。此外,短途迁徙者在西非表现出明显的季节性迁徙模式:1 - 5月向西南方向迁徙,6 - 7月向东北方向迁徙,8 - 12月向西南方向迁徙。长途迁徙者穿越撒哈拉沙漠的成功率极低(≤0.3%),仅发生在每年的2月至4月之间。结论本研究揭示了西非地区FAW的季节性迁移模式,为预测该地区的疫情风险和优化管理策略提供了重要见解。©2026作者。由John Wiley & Sons Ltd代表化学工业协会出版的《害虫管理科学》。
{"title":"Potential patterns of fall armyworm seasonal migration in West Africa modeled with atmospheric trajectory analyses.","authors":"Fan-Qi Gao,Xue-Yan Zhang,Rosina Kyerematen,Gao Hu,Regan Early,Jason W Chapman","doi":"10.1002/ps.70732","DOIUrl":"https://doi.org/10.1002/ps.70732","url":null,"abstract":"BACKGROUNDThe migratory invasive species fall armyworm (Spodoptera frugiperda, FAW) has established year-round populations in several West African countries following its initial invasion of Africa in early 2016. However, its seasonal migratory dynamics within West Africa remain poorly understood. If FAW populations in West Africa were able to successfully cross the Sahara Desert and serve as a major source population in North Africa, this could increase the risk of further invasion into southern Europe. In this study, we used atmospheric data to perform trajectory simulations, predicting the seasonal migratory pathways of short-distance migratory FAW individuals within West African breeding habitats and assessing the monthly probabilities of long-distance migrants departing from West Africa successfully crossing the Sahara Desert.RESULTSThe results indicate that from May to September, the vast majority of short-distance migrants (>70%) remained within West African breeding habitats, whereas in other months, a larger proportion of individuals were blown into the Atlantic Ocean. Moreover, short-distance migrants exhibited clear seasonal movement patterns within West Africa: shifting southwestward from January to May, turning northeastward in June and July, and returning southwestward from August to December. Long-distance migrants had an extremely low success rate (≤0.3%) of crossing the Sahara Desert, which occurred only between February and April each year.CONCLUSIONThis study revealed the seasonal migration patterns of FAW within West Africa, providing important insights for predicting regional outbreak risks and optimizing management strategies in the region. © 2026 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"20 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147495049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rational discovery of chitin deacetylase inhibitors against Botrytis cinerea via integrated computational approaches. 利用综合计算方法合理发现几丁质去乙酰化酶抑制剂。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-22 DOI: 10.1002/ps.70734
Donglin Xie,Juan Du
BACKGROUNDGray mold, caused by Botrytis cinerea, leading to great economic loss in agricultural production worldwide. Chemical fungicides represent one of the most effective strategies for preventing and controlling B. cinerea, while confronting increasingly severe resistance and environmental concerns. It is urgent to discover novel environment-friendly fungicides. Chitin deacetylase (CDA), an enzyme involved in chitin deacetylation, represents a promising novel target due to its role in helping pathogens evade plant immunity.RESULTSIn this work, we employed the AlphaFold3 server to predict the three-dimensional structure of B. cinerea chitin deacetylase (BcCDA). Structure based virtual screening identified the top eight compounds that bind to Zn2+ binding pocket of BcCDA. Then, molecular dynamics (MD) simulations and binding free energy calculations confirmed the stable interaction of candidate compounds with BcCDA and identified the residues crucial for the binding. Some of the candidates exhibited ecological safety and low environmental risk. Bioassays demonstrated that 105 244 924 and F0915-6107 showed inhibitory activity against BcCDA and effective prevention of B. cinerea invasion. Surface plasmon resonance (SPR) analysis confirmed the binding of 105 244 924 and F0915-6107 to residues Asp203 and His366 in the Zn2+ binding pocket of BcCDA, with KD values of 2.37 × 10-6 and 2.84 × 10-6 mol L-1, respectively.CONCLUSIONThese findings indicate that compounds 105 244 924 and F0915-6107 are promising candidates for developing antifungal agents against B. cinerea. © 2026 Society of Chemical Industry.
灰霉病是由灰霉病菌灰霉病引起的,在世界范围内造成了巨大的经济损失。化学杀菌剂是预防和控制绿僵菌最有效的策略之一,但面临着日益严重的耐药性和环境问题。开发新型环保型杀菌剂是当务之急。几丁质去乙酰化酶(CDA)是一种参与几丁质去乙酰化的酶,因其在帮助病原体逃避植物免疫中的作用而成为一种很有前景的新靶标。结果利用AlphaFold3服务器预测了灰芽孢杆菌几丁质脱乙酰酶(BcCDA)的三维结构。基于结构的虚拟筛选筛选出与BcCDA的Zn2+结合袋结合的前8个化合物。然后,分子动力学(MD)模拟和结合自由能计算证实了候选化合物与BcCDA的稳定相互作用,并确定了结合的关键残基。部分候选项目生态安全,环境风险低。生物实验表明,105 244 924和F0915-6107对BcCDA具有抑制作用,能有效地预防灰绿杆菌的侵袭。表面等离子体共振(SPR)分析证实,BcCDA Zn2+结合口袋中的105 244 924和F0915-6107与Asp203和His366残基结合,KD值分别为2.37 × 10-6和2.84 × 10-6 mol L-1。结论化合物105、244、924和F0915-6107是开发葡萄球菌抗真菌药物的理想候选药物。©2026化学工业协会。
{"title":"Rational discovery of chitin deacetylase inhibitors against Botrytis cinerea via integrated computational approaches.","authors":"Donglin Xie,Juan Du","doi":"10.1002/ps.70734","DOIUrl":"https://doi.org/10.1002/ps.70734","url":null,"abstract":"BACKGROUNDGray mold, caused by Botrytis cinerea, leading to great economic loss in agricultural production worldwide. Chemical fungicides represent one of the most effective strategies for preventing and controlling B. cinerea, while confronting increasingly severe resistance and environmental concerns. It is urgent to discover novel environment-friendly fungicides. Chitin deacetylase (CDA), an enzyme involved in chitin deacetylation, represents a promising novel target due to its role in helping pathogens evade plant immunity.RESULTSIn this work, we employed the AlphaFold3 server to predict the three-dimensional structure of B. cinerea chitin deacetylase (BcCDA). Structure based virtual screening identified the top eight compounds that bind to Zn2+ binding pocket of BcCDA. Then, molecular dynamics (MD) simulations and binding free energy calculations confirmed the stable interaction of candidate compounds with BcCDA and identified the residues crucial for the binding. Some of the candidates exhibited ecological safety and low environmental risk. Bioassays demonstrated that 105 244 924 and F0915-6107 showed inhibitory activity against BcCDA and effective prevention of B. cinerea invasion. Surface plasmon resonance (SPR) analysis confirmed the binding of 105 244 924 and F0915-6107 to residues Asp203 and His366 in the Zn2+ binding pocket of BcCDA, with KD values of 2.37 × 10-6 and 2.84 × 10-6 mol L-1, respectively.CONCLUSIONThese findings indicate that compounds 105 244 924 and F0915-6107 are promising candidates for developing antifungal agents against B. cinerea. © 2026 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"10 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147495050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of Brassica oleracea germplasm resources resistant to Plutella xylostella (L.). 甘蓝抗小菜蛾种质资源的鉴定。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-20 DOI: 10.1002/ps.70740
Peixuan Li,Liuhong Zhu,Hanchi Yang,Lina Dong,Shang Zhao,Honghao Lv,Sen Li,Neil Crickmore,Youjun Zhang,Zhaojiang Guo
BACKGROUNDBrassica oleracea includes many highly nutritious and widely consumed vegetable crops, yet it is severely threatened by Plutella xylostella, a notorious pest in agriculture. The insect-resistant traits developed by host plants over hundreds of millions of years provide one of the most promising methods for pest control, which is why we have screened germplasm for resistance to P. xylostella. Among 50 B. oleracea germplasm resources, we identified a key variety, DM187, that caused 53% mortality in larvae and inhibited the development and reproduction of this moth species.RESULTSIn this study, we collected and cultivated 50 B. oleracea germplasm resources and evaluated their resistance to P. xylostella. Among these germplasm resources, larvae feeding on DM187 caused the least leaf damage and exhibited the highest mortality rate. Moreover, DM187 significantly inhibited the larval length, larval weight, pupation rate, pupal weight, and adult egg production of surviving P. xylostella. Furthermore, ovarian anatomy showed that DM187 resulted in incomplete ovarian development and a reduced egg count in adult females.CONCLUSIONOur findings indicate that the B. oleracea germplasm DM187 significantly impairs the survival, development, and reproductive capacity of P. xylostella. These findings provide a theoretical basis for the identification and utilization of natural insect-resistant resources in vegetables, as well as for research on insect-resistant breeding, and offer a strategy for the effective management of pests in agricultural settings. © 2026 Society of Chemical Industry.
背景甘蓝包括许多高营养和广泛消费的蔬菜作物,但它受到小菜蛾的严重威胁,小菜蛾是一种臭名昭著的农业害虫。寄主植物数亿年来形成的抗虫性状为害虫防治提供了最有前途的方法之一,这就是我们筛选抗小菜蛾种质的原因。在50份甘蓝种质资源中,鉴定出一个关键品种DM187,该品种的幼虫死亡率为53%,并能抑制甘蓝蛾的发育和繁殖。结果本研究收集和培养了50份甘蓝种质资源,并对其对小菜蛾的抗性进行了评价。在这些种质资源中,取食DM187的幼虫对叶片的伤害最小,死亡率最高。DM187对小菜蛾幼虫的幼虫长、幼虫重、化蛹率、蛹重和成虫产蛋量均有显著抑制作用。此外,卵巢解剖显示DM187导致成年女性卵巢发育不完全和卵子数量减少。结论甘蓝种质DM187对小菜蛾的生存、发育和繁殖能力有显著影响。这些发现为蔬菜天然抗虫资源的鉴定和利用、抗虫育种研究提供了理论依据,并为农业环境下害虫的有效管理提供了策略。©2026化学工业协会。
{"title":"Identification of Brassica oleracea germplasm resources resistant to Plutella xylostella (L.).","authors":"Peixuan Li,Liuhong Zhu,Hanchi Yang,Lina Dong,Shang Zhao,Honghao Lv,Sen Li,Neil Crickmore,Youjun Zhang,Zhaojiang Guo","doi":"10.1002/ps.70740","DOIUrl":"https://doi.org/10.1002/ps.70740","url":null,"abstract":"BACKGROUNDBrassica oleracea includes many highly nutritious and widely consumed vegetable crops, yet it is severely threatened by Plutella xylostella, a notorious pest in agriculture. The insect-resistant traits developed by host plants over hundreds of millions of years provide one of the most promising methods for pest control, which is why we have screened germplasm for resistance to P. xylostella. Among 50 B. oleracea germplasm resources, we identified a key variety, DM187, that caused 53% mortality in larvae and inhibited the development and reproduction of this moth species.RESULTSIn this study, we collected and cultivated 50 B. oleracea germplasm resources and evaluated their resistance to P. xylostella. Among these germplasm resources, larvae feeding on DM187 caused the least leaf damage and exhibited the highest mortality rate. Moreover, DM187 significantly inhibited the larval length, larval weight, pupation rate, pupal weight, and adult egg production of surviving P. xylostella. Furthermore, ovarian anatomy showed that DM187 resulted in incomplete ovarian development and a reduced egg count in adult females.CONCLUSIONOur findings indicate that the B. oleracea germplasm DM187 significantly impairs the survival, development, and reproductive capacity of P. xylostella. These findings provide a theoretical basis for the identification and utilization of natural insect-resistant resources in vegetables, as well as for research on insect-resistant breeding, and offer a strategy for the effective management of pests in agricultural settings. © 2026 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"50 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147483706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification and field evaluation of sex pheromone components in the pine tip moth, Dioryctria sylvestrella (Lepidoptera: Pyralidae). 松尖蛾性信息素的鉴定与田间评价(鳞翅目:蛾科)。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-20 DOI: 10.1002/ps.70714
Zheng Wang,Yuge Zhao,Jiaxing Fang,Sufang Zhang,Jiayue Liu,Niannian Zhang,Fu Liu,Dafeng Chen,Xiangbo Kong
BACKGROUNDThe pine tip moth Dioryctria sylvestrella (Lepidoptera: Pyralidae) is a cambium miner of Mongolian Scots pine (Pinus sylvestris var. mongolica) and Korean pine (P. koraiensis), exerting devastating effects on forests throughout Eurasia. Larval galleries girdle the phloem, causing crown die-back and stand-scale mortality during outbreaks. Because larvae feed cryptically beneath the bark, contact insecticides seldom reach the target stage; consequently, infestations are usually detected only after economic injury has occurred. Therefore, a species-specific, early warning tool is urgently needed to effectively forecast population build-up and trigger precise interventions to avoid large-scale losses.RESULTSDioryctria sylvestrella pheromone gland profiling was performed by coupled gas chromatography (GC)-electroantennographic detection (EAD) and GC-mass spectrometry, and these results were reinforced by microderivatization with dimethyl disulfide and 4-methyl-1,2,4-triazoline-3,5-dione. A four-component sex pheromone released by calling D. sylvestrella females was identified comprising (Z)-9-tetradecenyl acetate (Z9-14:OAc). (9Z,11E)-tetradecadienyl acetate (Z9,E11-14:OAc), (Z)-11-hexadecenyl acetate (Z11-16:OAc), and (3Z,6Z,9Z,12Z,15Z)-pentacosapentaene (C25 pentaene) at a 1:2:10:10 ratio. The double-bond positions and geometries were determined according to retention times and diagnostic adduct ions. Field assays identified an optimal lure loading of 100:200:2000:1000 μg/dispenser of the four components. Heterospecific acetates (Z)-7-dodecenyl acetate (Z7-12:OAc) and (Z)-9-dodecenyl acetate (Z9-12:OAc), which are strong EAD stimulants, eliminated the trap efficacy, confirming their role as behavioral antagonists.CONCLUSIONWe report the first fully optimized, species-specific four-component sex pheromone for D. sylvestrella. The optimal formulation can be immediately deployed for region-wide monitoring, mass-trapping, and future mating-disruption programs against this cryptic forest pest. © 2026 Society of Chemical Industry.
松尖蛾(Dioryctria sylvestrella)是蒙古苏特松(Pinus sylvestris ar. mongolica)和红松(P. koraiensis)的形成层捕食者,对整个欧亚大陆的森林造成破坏性影响。幼虫通道环绕韧皮部,造成树冠枯死和林分规模死亡暴发期间。由于幼虫在树皮下隐蔽地进食,接触杀虫剂很少到达目标阶段;因此,通常只有在经济损失发生后才会发现虫害。因此,迫切需要一种特定物种的早期预警工具来有效预测种群增长并触发精确的干预措施,以避免大规模损失。结果采用气相色谱-天线电联用(GC)和气相色谱-质谱联用技术对二甲基二硫和4-甲基-1,2,4-三唑啉-3,5-二酮进行了微衍生化分析,并对结果进行了验证。鉴定出一种由(Z)-9-十四烯乙酸酯(Z9-14:OAc)组成的四组分性信息素。(9Z,11E)-乙酸十四烯酯(Z9,E11-14:OAc), (Z)-11-十六烯乙酸酯(Z11-16:OAc), (3Z,6Z,9Z,12Z,15Z)-五碳五烯(C25戊烯),比例为1:2:10:10。根据保留时间和诊断加合物离子确定双键位置和几何形状。现场试验确定四种成分的最佳诱捕量为100:200:2000:1000 μg/剂。异种乙酸酯(Z)-7-十二烷基乙酸酯(Z7-12:OAc)和(Z)-9-十二烷基乙酸酯(Z9-12:OAc)是强EAD刺激物,消除了诱捕作用,证实了它们作为行为拮抗剂的作用。结论报道了首个完全优化的、具有种特异性的四组分性信息素。最佳配方可以立即用于区域范围内的监测、大规模捕获和未来针对这种隐蔽性森林害虫的交配中断计划。©2026化学工业协会。
{"title":"Identification and field evaluation of sex pheromone components in the pine tip moth, Dioryctria sylvestrella (Lepidoptera: Pyralidae).","authors":"Zheng Wang,Yuge Zhao,Jiaxing Fang,Sufang Zhang,Jiayue Liu,Niannian Zhang,Fu Liu,Dafeng Chen,Xiangbo Kong","doi":"10.1002/ps.70714","DOIUrl":"https://doi.org/10.1002/ps.70714","url":null,"abstract":"BACKGROUNDThe pine tip moth Dioryctria sylvestrella (Lepidoptera: Pyralidae) is a cambium miner of Mongolian Scots pine (Pinus sylvestris var. mongolica) and Korean pine (P. koraiensis), exerting devastating effects on forests throughout Eurasia. Larval galleries girdle the phloem, causing crown die-back and stand-scale mortality during outbreaks. Because larvae feed cryptically beneath the bark, contact insecticides seldom reach the target stage; consequently, infestations are usually detected only after economic injury has occurred. Therefore, a species-specific, early warning tool is urgently needed to effectively forecast population build-up and trigger precise interventions to avoid large-scale losses.RESULTSDioryctria sylvestrella pheromone gland profiling was performed by coupled gas chromatography (GC)-electroantennographic detection (EAD) and GC-mass spectrometry, and these results were reinforced by microderivatization with dimethyl disulfide and 4-methyl-1,2,4-triazoline-3,5-dione. A four-component sex pheromone released by calling D. sylvestrella females was identified comprising (Z)-9-tetradecenyl acetate (Z9-14:OAc). (9Z,11E)-tetradecadienyl acetate (Z9,E11-14:OAc), (Z)-11-hexadecenyl acetate (Z11-16:OAc), and (3Z,6Z,9Z,12Z,15Z)-pentacosapentaene (C25 pentaene) at a 1:2:10:10 ratio. The double-bond positions and geometries were determined according to retention times and diagnostic adduct ions. Field assays identified an optimal lure loading of 100:200:2000:1000 μg/dispenser of the four components. Heterospecific acetates (Z)-7-dodecenyl acetate (Z7-12:OAc) and (Z)-9-dodecenyl acetate (Z9-12:OAc), which are strong EAD stimulants, eliminated the trap efficacy, confirming their role as behavioral antagonists.CONCLUSIONWe report the first fully optimized, species-specific four-component sex pheromone for D. sylvestrella. The optimal formulation can be immediately deployed for region-wide monitoring, mass-trapping, and future mating-disruption programs against this cryptic forest pest. © 2026 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"23 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147490143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Azaspiromycin and pentalenolactone derivatives with insecticidal activities against Plutella xylostella from Streptomyces huasconensis NA10. 阿扎匹霉素和戊烯内酯衍生物对花锥链霉菌小菜蛾的杀虫活性研究
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-20 DOI: 10.1002/ps.70751
Yan Bai,Meiyan Tan,Hailiang Sun,Xiaoke Wang,Xiaoyu Song,Jiangchun Hu,Huaqi Pan
BACKGROUNDPlutella xylostella larvae constitute the most destructive pest of cruciferous crops globally and have caused huge economic losses. However, chemical pesticides for their control are toxic to the ecological environment and there is pest resistance against them. Searching for compounds with insecticidal activity that are environmentally friendly has become imperative.RESULTSCrude extracts of Streptomyces huasconensis NA10 showed significant insecticidal activities against P. xylostella larvae, and genomic mining was used to predict that this bacterial strain could produce potentially undescribed insecticidal compounds. To discover new natural biopesticides, three new compounds, azaspiromycin, pentalenolactone J and neopentalenolactone G, along with one known compound, pentalenolactone O, were isolated from marine-derived S. huasconensis NA10, guided by insecticidal activity screening. Their structures were identified through comprehensive spectroscopic data analysis and quantum chemical calculations. Remarkably, azaspiromycin contains a unique spiro-tetrahydropyran-γ-lactam bicyclic skeleton, previously unreported in natural products. Furthermore, plausible biosynthetic pathways of four compounds were deduced. In the insecticidal activity assay, these compounds showed moderate-to-strong insecticidal effects [median lethal dose (LD50) = 5.1-25.5 μm]. Among them, azaspiromycin and pentalenolactone O demonstrated significant insecticidal effects (LD50 = 8.3 and 5.1 μm), comparable to the positive control abamectin (LD50 = 4.2 μm). The result of acute toxicity further indicated that these compounds were safe for a nontarget organism (silkworm). This is the first report on the insecticidal efficacy of azaspiromycin and pentalenolactone derivatives against P. xylostella larvae.CONCLUSIONThis study demonstrates the potential of azaspiromycin and pentalenolactone derivatives as novel biopesticides for effective P. xylostella control. © 2026 Society of Chemical Industry.
背景小菜蛾幼虫是全球十字花科作物最具破坏性的害虫,造成了巨大的经济损失。但化学农药对生态环境有毒害作用,且对其有抗性。寻找具有杀虫活性且对环境友好的化合物已成为当务之急。结果花conconstreptomyces huasconensis NA10粗提物对小菜蛾幼虫有明显的杀虫活性,利用基因组挖掘技术预测该菌株可能产生未被描述的杀虫化合物。为了发现新的天然生物农药,从海洋来源的花螺NA10中分离到阿扎霉素、戊烯内酯J和新戊烯内酯G三个新化合物,并对已知化合物戊烯内酯O进行杀虫活性筛选。通过全面的光谱数据分析和量子化学计算确定了它们的结构。值得注意的是,阿斯匹霉素含有一个独特的螺-四氢吡喃-γ-内酰胺双环骨架,以前未在天然产物中报道过。进一步推导了四种化合物的生物合成途径。在杀虫活性测定中,这些化合物表现出中强杀虫作用[中位致死剂量(LD50) = 5.1 ~ 25.5 μm]。其中阿沙匹霉素和戊烯内酯O的杀虫效果显著(LD50分别为8.3和5.1 μm),与阳性对照阿维菌素(LD50分别为4.2 μm)相当。急性毒性试验结果进一步表明,这些化合物对非靶生物(家蚕)是安全的。本文首次报道了阿扎匹霉素和戊烯内酯衍生物对小菜蛾幼虫的杀虫效果。结论阿斯匹霉素和戊烯内酯衍生物具有作为新型生物农药防治小菜蛾的潜力。©2026化学工业协会。
{"title":"Azaspiromycin and pentalenolactone derivatives with insecticidal activities against Plutella xylostella from Streptomyces huasconensis NA10.","authors":"Yan Bai,Meiyan Tan,Hailiang Sun,Xiaoke Wang,Xiaoyu Song,Jiangchun Hu,Huaqi Pan","doi":"10.1002/ps.70751","DOIUrl":"https://doi.org/10.1002/ps.70751","url":null,"abstract":"BACKGROUNDPlutella xylostella larvae constitute the most destructive pest of cruciferous crops globally and have caused huge economic losses. However, chemical pesticides for their control are toxic to the ecological environment and there is pest resistance against them. Searching for compounds with insecticidal activity that are environmentally friendly has become imperative.RESULTSCrude extracts of Streptomyces huasconensis NA10 showed significant insecticidal activities against P. xylostella larvae, and genomic mining was used to predict that this bacterial strain could produce potentially undescribed insecticidal compounds. To discover new natural biopesticides, three new compounds, azaspiromycin, pentalenolactone J and neopentalenolactone G, along with one known compound, pentalenolactone O, were isolated from marine-derived S. huasconensis NA10, guided by insecticidal activity screening. Their structures were identified through comprehensive spectroscopic data analysis and quantum chemical calculations. Remarkably, azaspiromycin contains a unique spiro-tetrahydropyran-γ-lactam bicyclic skeleton, previously unreported in natural products. Furthermore, plausible biosynthetic pathways of four compounds were deduced. In the insecticidal activity assay, these compounds showed moderate-to-strong insecticidal effects [median lethal dose (LD50) = 5.1-25.5 μm]. Among them, azaspiromycin and pentalenolactone O demonstrated significant insecticidal effects (LD50 = 8.3 and 5.1 μm), comparable to the positive control abamectin (LD50 = 4.2 μm). The result of acute toxicity further indicated that these compounds were safe for a nontarget organism (silkworm). This is the first report on the insecticidal efficacy of azaspiromycin and pentalenolactone derivatives against P. xylostella larvae.CONCLUSIONThis study demonstrates the potential of azaspiromycin and pentalenolactone derivatives as novel biopesticides for effective P. xylostella control. © 2026 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"12 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147483705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Thirty years of glyphosate-resistant crops and weeds: Current situation and future prospects. 抗草甘膦作物和杂草三十年:现状和未来展望。
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-19 DOI: 10.1002/ps.70742
Ricardo Alcántara-de la Cruz,Yure Marin Guidi,Mariana Ramirez-Castillo,Fernando Storniolo Adegas,Caio Antonio Carbonari,Edivaldo Domingues Velini,Rafael De Prado,Stephen O Duke
Glyphosate, a broad-spectrum herbicide targeting 5-enolpyruvylshikimate-3-phosphate synthase, was first commercialized in 1974. Its use increased significantly following the introduction of glyphosate-resistant (GR) crops in 1996 and the expiration of its patent in 2000, making it the most used herbicide worldwide. Intensive reliance on glyphosate has selected for GR weed populations, with the first documented case reported also in 1996. Glyphosate resistance now has evolved in 62 weed species across 31 countries, often involving multiple other herbicide modes of action and thereby complicating chemical control. The highest incidence has occurred in regions with extensive GR crop cultivation, particularly the United States, Brazil, and Argentina, where glyphosate has been used for years as the sole or major herbicide. Apparent disparities among countries largely reflect differences in the timing and reporting of first unique resistance cases, rather than actual differences in the overall prevalence of GR weeds. GR weeds possess the greatest diversity of resistance mechanisms described for any herbicide, encompassing both target-site and non-target-site resistance mechanisms, frequently in combination. In many cases, resistance traits that provide low resistance levels have combined over time to provide more robust resistance (creeping resistance). Evolution and spread of glyphosate resistance are influenced by frequency of use, herbicide dose, phenological stage, pollination biology, gene flow, and dissemination of GR seeds or propagules via contaminated seed/grain lots and machinery. Glyphosate resistance and controversies regarding environmental and health issues have so far not resulted in marked reductions in glyphosate use nor in the introduction of a herbicide or other technology that is as effective and economical as glyphosate in managing weeds. This review synthesizes current knowledge of glyphosate resistance and its mechanisms, highlighting existing gaps, and discusses potential scenarios for the future of glyphosate and GR crops. © 2026 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
草甘膦是一种针对5-烯醇丙酮酰基莽草-3-磷酸合成酶的广谱除草剂,于1974年首次商业化。随着1996年抗草甘膦(GR)作物的引入和2000年其专利到期,其使用量显著增加,使其成为全球使用最多的除草剂。对草甘膦的高度依赖已成为GR杂草种群的选择,1996年也报告了第一例有记录的病例。目前,31个国家的62种杂草已经进化出对草甘膦的抗性,通常涉及多种其他除草剂的作用模式,从而使化学控制复杂化。发病率最高的地区是广泛种植转基因作物的地区,特别是美国、巴西和阿根廷,这些地区多年来一直将草甘膦作为唯一或主要的除草剂使用。各国之间的明显差异在很大程度上反映了首次独特耐药性病例的时间和报告的差异,而不是GR杂草总体流行率的实际差异。GR杂草具有任何除草剂所描述的最多样化的抗性机制,包括靶点和非靶点抗性机制,经常是联合抗性机制。在许多情况下,提供低抗性水平的抗性性状随着时间的推移结合在一起,提供更强大的抗性(蠕变抗性)。草甘膦抗性的进化和传播受到使用频率、除草剂剂量、物候阶段、授粉生物学、基因流动以及通过受污染的种子/谷物批次和机械传播GR种子或繁殖体的影响。迄今为止,对草甘膦的抗性以及有关环境和健康问题的争议并未导致草甘膦的使用显著减少,也没有引入与草甘膦一样有效和经济的除草剂或其他技术来管理杂草。这篇综述综合了目前关于草甘膦抗性及其机制的知识,突出了现有的空白,并讨论了草甘膦和转基因作物未来的潜在前景。©2026作者。由John Wiley & Sons Ltd代表化学工业协会出版的《害虫管理科学》。
{"title":"Thirty years of glyphosate-resistant crops and weeds: Current situation and future prospects.","authors":"Ricardo Alcántara-de la Cruz,Yure Marin Guidi,Mariana Ramirez-Castillo,Fernando Storniolo Adegas,Caio Antonio Carbonari,Edivaldo Domingues Velini,Rafael De Prado,Stephen O Duke","doi":"10.1002/ps.70742","DOIUrl":"https://doi.org/10.1002/ps.70742","url":null,"abstract":"Glyphosate, a broad-spectrum herbicide targeting 5-enolpyruvylshikimate-3-phosphate synthase, was first commercialized in 1974. Its use increased significantly following the introduction of glyphosate-resistant (GR) crops in 1996 and the expiration of its patent in 2000, making it the most used herbicide worldwide. Intensive reliance on glyphosate has selected for GR weed populations, with the first documented case reported also in 1996. Glyphosate resistance now has evolved in 62 weed species across 31 countries, often involving multiple other herbicide modes of action and thereby complicating chemical control. The highest incidence has occurred in regions with extensive GR crop cultivation, particularly the United States, Brazil, and Argentina, where glyphosate has been used for years as the sole or major herbicide. Apparent disparities among countries largely reflect differences in the timing and reporting of first unique resistance cases, rather than actual differences in the overall prevalence of GR weeds. GR weeds possess the greatest diversity of resistance mechanisms described for any herbicide, encompassing both target-site and non-target-site resistance mechanisms, frequently in combination. In many cases, resistance traits that provide low resistance levels have combined over time to provide more robust resistance (creeping resistance). Evolution and spread of glyphosate resistance are influenced by frequency of use, herbicide dose, phenological stage, pollination biology, gene flow, and dissemination of GR seeds or propagules via contaminated seed/grain lots and machinery. Glyphosate resistance and controversies regarding environmental and health issues have so far not resulted in marked reductions in glyphosate use nor in the introduction of a herbicide or other technology that is as effective and economical as glyphosate in managing weeds. This review synthesizes current knowledge of glyphosate resistance and its mechanisms, highlighting existing gaps, and discusses potential scenarios for the future of glyphosate and GR crops. © 2026 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"16 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147483709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seedling resistance and chemical defenses against Psylliodes chrysocephala : the roles of seed age and sinapinic acid in Sinapis alba and Brassica napus 幼苗对黄头木虱的抗性和化学防御:种子龄和芥子酸在白芥和甘蓝型油菜中的作用
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-19 DOI: 10.1002/ps.70724
Daniel Rüde, Samantha M Cook, Bernd Ulber, Tobias Pöhnl, Michael Rostás
BACKGROUND The cabbage stem flea beetle, Psylliodes chrysocephala , is a major pest of winter oilseed rape ( Brassica napus ) in Europe. Adults feed on cotyledons and young leaves, threatening the establishment and vigor of the crop. With no insect‐resistant cultivars of B. napus currently available, farmers must rely primarily on synthetic insecticides for pest control. This study investigated the resistance of Brassica napus and Sinapis alba seedlings to adult P. chrysocephala feeding and explored the underlying chemical defense mechanisms. Seedlings from three B. napus accessions, including two with contrasting seed ages, and three S. alba accessions were evaluated for feeding damage in controlled laboratory conditions, while a subset of two B. napus and two S. alba accessions was further evaluated in semi‐field assays. Central metabolomic and glucosinolate profiling was conducted to identify resistance‐linked compounds. RESULTS Under controlled conditions, S. alba seedlings exhibited substantially less feeding damage compared to a standard B. napus cultivar under controlled conditions. Notably, older B. napus seeds stored for 6 or 9 years produced seedlings with significantly reduced susceptibility to herbivory, coinciding with changes in central and specialized metabolite profiles, compared to freshly harvested seeds (younger than one year). Glucosinolate analyses revealed species‐specific profiles, while dual‐choice bioassays identified sinapinic acid as a feeding deterrent to P. chrysocephala adults. CONCLUSION We successfully identified variation in cotyledon‐stage resistance to P. chrysocephala among Brassicaceae species. These findings highlight the potential of S. alba as a source of resistance traits and suggest that seed physiology influences seedling resistance, offering new avenues for breeding insect‐resistant oilseed crops, which are needed for integrated pest management. © 2026 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
背景白菜茎蚤是欧洲冬季油菜(Brassica napus)的主要害虫。成虫以子叶和嫩叶为食,威胁着作物的生长和活力。由于目前没有抗虫的甘蓝型油菜品种,农民必须主要依靠合成杀虫剂进行虫害防治。研究了甘蓝型油菜和白刺幼苗对黄头青虫成虫取食的抗性,并探讨了其化学防御机制。在受控的实验室条件下,对三种甘蓝型油菜(包括两种种子龄不同的甘蓝型油菜)和三种白僵菌(S. alba)的幼苗进行了取食损害评估,同时在半田试验中对两种甘蓝型油菜和两种白僵菌的幼苗进行了进一步评估。进行中心代谢组学和硫代葡萄糖苷谱分析以鉴定耐药性相关化合物。结果在控制条件下,与标准甘蓝型油菜品种相比,白僵菌幼苗的取食损害明显减轻。值得注意的是,与新鲜收获的种子(不到一年)相比,储存6年或9年的甘蓝型油菜种子产生的幼苗对草食的敏感性显著降低,这与中央和专门代谢物谱的变化相一致。硫代葡萄糖苷分析揭示了物种特异性特征,而双选择生物测定鉴定了皂素酸对黄头假蝇成虫的取食威慑作用。结论成功鉴定了芸苔科植物子叶期对黄头霉病抗性的差异。这些发现突出了S. alba作为抗性性状来源的潜力,并表明种子生理影响幼苗抗性,为培育抗虫油籽作物提供了新的途径,这是病虫害综合治理所需要的。©2026作者。《有害生物管理科学》由John Wiley &; Sons Ltd代表化学工业协会出版。
{"title":"Seedling resistance and chemical defenses against Psylliodes chrysocephala : the roles of seed age and sinapinic acid in Sinapis alba and Brassica napus ","authors":"Daniel Rüde, Samantha M Cook, Bernd Ulber, Tobias Pöhnl, Michael Rostás","doi":"10.1002/ps.70724","DOIUrl":"https://doi.org/10.1002/ps.70724","url":null,"abstract":"BACKGROUND The cabbage stem flea beetle, <jats:italic>Psylliodes chrysocephala</jats:italic> , is a major pest of winter oilseed rape ( <jats:italic>Brassica napus</jats:italic> ) in Europe. Adults feed on cotyledons and young leaves, threatening the establishment and vigor of the crop. With no insect‐resistant cultivars of <jats:italic>B. napus</jats:italic> currently available, farmers must rely primarily on synthetic insecticides for pest control. This study investigated the resistance of <jats:italic>Brassica napus</jats:italic> and <jats:italic>Sinapis alba</jats:italic> seedlings to adult <jats:italic>P. chrysocephala</jats:italic> feeding and explored the underlying chemical defense mechanisms. Seedlings from three <jats:italic>B. napus</jats:italic> accessions, including two with contrasting seed ages, and three <jats:italic>S. alba</jats:italic> accessions were evaluated for feeding damage in controlled laboratory conditions, while a subset of two <jats:italic>B. napus</jats:italic> and two <jats:italic>S. alba</jats:italic> accessions was further evaluated in semi‐field assays. Central metabolomic and glucosinolate profiling was conducted to identify resistance‐linked compounds. RESULTS Under controlled conditions, <jats:italic>S. alba</jats:italic> seedlings exhibited substantially less feeding damage compared to a standard <jats:italic>B. napus</jats:italic> cultivar under controlled conditions. Notably, older <jats:italic>B. napus</jats:italic> seeds stored for 6 or 9 years produced seedlings with significantly reduced susceptibility to herbivory, coinciding with changes in central and specialized metabolite profiles, compared to freshly harvested seeds (younger than one year). Glucosinolate analyses revealed species‐specific profiles, while dual‐choice bioassays identified sinapinic acid as a feeding deterrent to <jats:italic>P. chrysocephala</jats:italic> adults. CONCLUSION We successfully identified variation in cotyledon‐stage resistance to <jats:italic>P. chrysocephala</jats:italic> among Brassicaceae species. These findings highlight the potential of <jats:italic>S. alba</jats:italic> as a source of resistance traits and suggest that seed physiology influences seedling resistance, offering new avenues for breeding insect‐resistant oilseed crops, which are needed for integrated pest management. © 2026 The Author(s). <jats:italic>Pest Management Science</jats:italic> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"44 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147478704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Edge effects, tree cover and soil properties linked to the distribution of Diaprepes abbreviatus in a Florida citrus orchard 边缘效应、树木覆盖和土壤性质与佛罗里达柑橘果园中短叶霉分布的关系
IF 4.1 1区 农林科学 Q1 AGRONOMY Pub Date : 2026-03-19 DOI: 10.1002/ps.70723
Homan Regmi, Alexandros Dritsoulas, Shokoofeh Kamali, Lukasz L. Stelinski, Lauren M. Diepenbrock, Larry Duncan
BACKGROUND Diaprepes root weevil (DRW, Diaprepes abbreviatus ) is a major economic pest of citrus trees in Florida and the Caribbean Basin. To identify potential drivers of local patterns of weevil abundance and tree condition, we measured relationships between DRW and edaphic properties in a Florida orchard in which half the trees were initially protected from herbivores by individual protective covers (IPCs) of fabric mesh. Weevils were monitored for 2 years in 94 plots arranged in a grid pattern. Soil samples were processed for physicochemical properties, and DNA from soil organisms was subjected to metabarcoding for ITS2 rDNA, 16S rDNA, and COI mtDNA. RESULTS Weevils aggregated each summer along plot boundaries adjacent to a natural area of alternate hosts, consistent with an edge‐biased distribution reflecting seasonal weevil migration. Fourteen months after the covers were removed, 93% of 484 prokaryote species (dominated by Bacilli, Actinobacteria, Alphaproteobacteria, and Cyanobacteria) that were most closely associated with IPC use were less abundant beneath the trees that were formerly covered than under previously uncovered trees. Soil moisture, Paenibacillus spp. diversity, and IPCs were associated with DRW patterns and tree condition including mortality. CONCLUSIONS Early season, edge‐biased distribution of DRW should be exploited for monitoring and management efficacy. The relationship here between tree mortality and microbiome species deficits associated with IPCs supports the need for research to resolve the role of tree covers in potential trophic cascades that affect plant health. © 2026 Society of Chemical Industry.
根象甲(DRW, Diaprepes缩写)是佛罗里达和加勒比海盆地柑橘树的主要经济害虫。为了确定当地象牙虫丰度和树木状况的潜在驱动因素,我们测量了佛罗里达州一个果园的DRW与土壤特性之间的关系,该果园最初有一半的树木被单独的防护罩(IPCs)保护,不受食草动物的侵害。采用网格法对94个样地进行了2年的象鼻虫监测。对土壤样品进行理化性质处理,并对土壤生物的DNA进行ITS2 rDNA、16S rDNA和COI mtDNA的元条形码编码。结果:象鼻虫每年夏季聚集在相邻交替寄主自然区域的样地边界上,与反映季节性象鼻虫迁移的边缘偏倚分布一致。覆盖物移除14个月后,与IPC使用最密切相关的484种原核生物(以杆菌、放线菌、α变形菌和蓝藻菌为主)中,有93%在以前被覆盖物覆盖的树木下的数量少于以前未覆盖的树木。土壤湿度、芽孢杆菌多样性和IPCs与DRW模式和树木状况(包括死亡率)相关。结论应利用DRW的早期偏边分布来监测和管理效果。树木死亡率与与IPCs相关的微生物组物种缺失之间的关系支持了研究解决树木覆盖在影响植物健康的潜在营养级联中的作用的必要性。©2026化学工业协会。
{"title":"Edge effects, tree cover and soil properties linked to the distribution of Diaprepes abbreviatus in a Florida citrus orchard","authors":"Homan Regmi, Alexandros Dritsoulas, Shokoofeh Kamali, Lukasz L. Stelinski, Lauren M. Diepenbrock, Larry Duncan","doi":"10.1002/ps.70723","DOIUrl":"https://doi.org/10.1002/ps.70723","url":null,"abstract":"BACKGROUND Diaprepes root weevil (DRW, <jats:italic>Diaprepes abbreviatus</jats:italic> ) is a major economic pest of citrus trees in Florida and the Caribbean Basin. To identify potential drivers of local patterns of weevil abundance and tree condition, we measured relationships between DRW and edaphic properties in a Florida orchard in which half the trees were initially protected from herbivores by individual protective covers (IPCs) of fabric mesh. Weevils were monitored for 2 years in 94 plots arranged in a grid pattern. Soil samples were processed for physicochemical properties, and DNA from soil organisms was subjected to metabarcoding for ITS2 rDNA, 16S rDNA, and COI mtDNA. RESULTS Weevils aggregated each summer along plot boundaries adjacent to a natural area of alternate hosts, consistent with an edge‐biased distribution reflecting seasonal weevil migration. Fourteen months after the covers were removed, 93% of 484 prokaryote species (dominated by Bacilli, Actinobacteria, Alphaproteobacteria, and Cyanobacteria) that were most closely associated with IPC use were less abundant beneath the trees that were formerly covered than under previously uncovered trees. Soil moisture, <jats:italic>Paenibacillus</jats:italic> spp. diversity, and IPCs were associated with DRW patterns and tree condition including mortality. CONCLUSIONS Early season, edge‐biased distribution of DRW should be exploited for monitoring and management efficacy. The relationship here between tree mortality and microbiome species deficits associated with IPCs supports the need for research to resolve the role of tree covers in potential trophic cascades that affect plant health. © 2026 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"6 2 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147478097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Pest Management Science
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1