Pub Date : 2023-01-01DOI: 10.1016/j.smaim.2022.09.004
Rawand A. Mustafa , Meixin Ran , Yonghui Wang , Jiaqi Yan , Yu Zhang , Jessica M. Rosenholm , Hongbo Zhang
To optimize synergistic breast cancer treatment, a nanocomposite was fabricated with pH-temperature responsive and chemo-photothermal combination therapy. Herein, gold nanorods (AuNRs) are coated with [poly[(N-isopropylacrylamide)-co-(methacrylic acid)] (p(NIPAM-co-MAA)) modified mesoporous silica (MS) for Doxorubicin (DOX) delivery (AuNR@DOX-MS@p(NIPAM-co-MAA)). Upon NIR radiation, the AuNR core induced hyperthermia via generating heat. Simultaneously, the polymer layer collapsed in response to high temperature/low pH, which allowed the triggering of DOX release from the MS shell at the tumor site. With this nanocomposite, nearly zero premature release of DOX at physiological pH/temperature was detected, while effective DOX release was reported at higher temperature/lower pH values. In addition, in vitro studies demonstrated that the nanocomposite has a substantial uptake efficiency of MDA-MB-231 breast cancer cells, with a significant increase in suppressing MDA-MB-231 cell proliferation in response to laser irradiation. The in vivo experiments further verified the high efficiency of the fabricated nanocomposite in accumulating at the tumor site and the good capability in suppressing tumor growth in the mice upon intravenous injection, while exhibiting good biosafety in relation to major organs in the body. Thus, the synthesized nanocomposite could be a potential nanocarrier for breast cancer treatment with synergistic chemo-photothermal therapeutic capability.
{"title":"A pH/temperature responsive nanocomposite for chemo-photothermal synergistic cancer therapy","authors":"Rawand A. Mustafa , Meixin Ran , Yonghui Wang , Jiaqi Yan , Yu Zhang , Jessica M. Rosenholm , Hongbo Zhang","doi":"10.1016/j.smaim.2022.09.004","DOIUrl":"10.1016/j.smaim.2022.09.004","url":null,"abstract":"<div><p>To optimize synergistic breast cancer treatment, a nanocomposite was fabricated with pH-temperature responsive and chemo-photothermal combination therapy. Herein, gold nanorods (AuNRs) are coated with [poly[(N-isopropylacrylamide)-co-(methacrylic acid)] (p(NIPAM-co-MAA)) modified mesoporous silica (MS) for Doxorubicin (DOX) delivery (AuNR@DOX-MS@p(NIPAM-co-MAA)). Upon NIR radiation, the AuNR core induced hyperthermia via generating heat. Simultaneously, the polymer layer collapsed in response to high temperature/low pH, which allowed the triggering of DOX release from the MS shell at the tumor site. With this nanocomposite, nearly zero premature release of DOX at physiological pH/temperature was detected, while effective DOX release was reported at higher temperature/lower pH values. In addition, <em>in vitro</em> studies demonstrated that the nanocomposite has a substantial uptake efficiency of MDA-MB-231 breast cancer cells, with a significant increase in suppressing MDA-MB-231 cell proliferation in response to laser irradiation. The <em>in vivo</em> experiments further verified the high efficiency of the fabricated nanocomposite in accumulating at the tumor site and the good capability in suppressing tumor growth in the mice upon intravenous injection, while exhibiting good biosafety in relation to major organs in the body. Thus, the synthesized nanocomposite could be a potential nanocarrier for breast cancer treatment with synergistic chemo-photothermal therapeutic capability.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":"4 ","pages":"Pages 199-211"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44475381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1016/j.smaim.2023.01.002
Rui Zan , Sheng Shen , Yuanding Huang , Han Yu , Yaohui Liu , Shi Yang , Bohao Zheng , Zijun Gong , Wenhui Wang , Xiaonong Zhang , Tao Suo , Houbao Liu
With the increasing demand for innovative therapies, biodegradable magnesium has attracted more and more attention, which could avoid secondary surgery and reduce complications. Until now, plenty of researchers take part in the research & development of this field, and many articles have been published every year. However, it is a huge challenge to predict research trends and definite topics for researchers, which could result in low research value, wasted resources and even slowed medical device transformation. Usually, reviews summarize a specific topic, such as alloy elements, coating designs, degradable properties, etc. Deriving key indicators from a large amount of data with the help of statistical analysis, make a historical review, current situation analysis, and future prediction more convincing. Herein, it has been conducted a bibliometric study according to 2669 publications collected from the Web of Science (WOS) database from 2005 to 2021. By analyzing some key factors, including annual publications, keywords, country contributions, authors as well as institutions, the evolution of biodegradable magnesium is objectively studied. The research trends of biodegradable magnesium alloys are corrosion resistance, the influence of microstructural control on mechanical behavior and bio-functions of implants in a chronological manner. The co-occurrence mapping of the countries and authors suggests that current in-depth research and development of magnesium is more emphasis on institutional and international cooperation.
随着创新疗法需求的增加,生物可降解镁因其可避免二次手术和减少并发症而受到越来越多的关注。到目前为止,许多研究人员都参与了这项研究。这一领域的发展,每年都有许多文章发表。然而,对研究人员来说,预测研究趋势和确定研究主题是一个巨大的挑战,这可能导致研究价值低,资源浪费,甚至减缓医疗器械的转型。通常,综述总结一个特定的主题,如合金元素,涂层设计,可降解性能等。借助统计分析,从大量数据中得出关键指标,使历史回顾、现状分析、未来预测更具说服力。本文对Web of Science (WOS)数据库2005 - 2021年收录的2669篇文献进行了文献计量学研究。通过对年度出版物、关键词、国家贡献、作者、机构等关键因素的分析,客观地研究了生物可降解镁的发展历程。生物可降解镁合金的研究方向依次为耐腐蚀性能、微观结构控制对植入物力学行为和生物功能的影响。国家和作者的共现图表明,目前镁的深入研究和开发更加注重机构和国际合作。
{"title":"Research hotspots and trends of biodegradable magnesium and its alloys","authors":"Rui Zan , Sheng Shen , Yuanding Huang , Han Yu , Yaohui Liu , Shi Yang , Bohao Zheng , Zijun Gong , Wenhui Wang , Xiaonong Zhang , Tao Suo , Houbao Liu","doi":"10.1016/j.smaim.2023.01.002","DOIUrl":"10.1016/j.smaim.2023.01.002","url":null,"abstract":"<div><p>With the increasing demand for innovative therapies, biodegradable magnesium has attracted more and more attention, which could avoid secondary surgery and reduce complications. Until now, plenty of researchers take part in the research & development of this field, and many articles have been published every year. However, it is a huge challenge to predict research trends and definite topics for researchers, which could result in low research value, wasted resources and even slowed medical device transformation. Usually, reviews summarize a specific topic, such as alloy elements, coating designs, degradable properties, etc. Deriving key indicators from a large amount of data with the help of statistical analysis, make a historical review, current situation analysis, and future prediction more convincing. Herein, it has been conducted a bibliometric study according to 2669 publications collected from the Web of Science (WOS) database from 2005 to 2021. By analyzing some key factors, including annual publications, keywords, country contributions, authors as well as institutions, the evolution of biodegradable magnesium is objectively studied. The research trends of biodegradable magnesium alloys are corrosion resistance, the influence of microstructural control on mechanical behavior and bio-functions of implants in a chronological manner. The co-occurrence mapping of the countries and authors suggests that current in-depth research and development of magnesium is more emphasis on institutional and international cooperation.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":"4 ","pages":"Pages 468-479"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44148398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1016/j.smaim.2022.10.002
Uddeshya Shukla , Kamal Garg
This review paper deals with the advancements of composites to shape memory alloys. The journey of smart materials from conventional composites to advance shape memory alloys and their application is described in this literature. Classification of smart materials such as smart composites, shape memory alloys, polymer composite and various other types of materials that are intelligent are explained briefly. Different manufacturing and developing techniques to manufacture smart materials and characterization of conventional composites is compared with advance modern day shape memory alloys. Shape memory effect such as one way and two-way shape memory effect are depicted. However, the most important of all the applications and extensive use of smart materials in health care sector for implants and various other uses with uses in aerospace and automotive industries are reviewed.
{"title":"Journey of smart material from composite to shape memory alloy (SMA), characterization and their applications-A review","authors":"Uddeshya Shukla , Kamal Garg","doi":"10.1016/j.smaim.2022.10.002","DOIUrl":"10.1016/j.smaim.2022.10.002","url":null,"abstract":"<div><p>This review paper deals with the advancements of composites to shape memory alloys. The journey of smart materials from conventional composites to advance shape memory alloys and their application is described in this literature. Classification of smart materials such as smart composites, shape memory alloys, polymer composite and various other types of materials that are intelligent are explained briefly. Different manufacturing and developing techniques to manufacture smart materials and characterization of conventional composites is compared with advance modern day shape memory alloys. Shape memory effect such as one way and two-way shape memory effect are depicted. However, the most important of all the applications and extensive use of smart materials in health care sector for implants and various other uses with uses in aerospace and automotive industries are reviewed.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":"4 ","pages":"Pages 227-242"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46162639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1016/j.smaim.2022.11.008
Yi-Tung Lu , Pei-Tzu Hung , Kui Zeng , Christian Woelk , Bodo Fuhrmann , Kai Zhang , Thomas Groth
The thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) is widely applied in the biomedical field particularly as thermoresponsive substrate for culture of cells. To be used as a stimuli-responsive coating for cell culture, combining PNIPAM with glycosaminoglycans might be an effective approach to improve its bioactivity. In this study, chitosan is grafted with PNIPAM moieties (PCHI) possessing a cloud point at 31 °C and used as a polycation to fabricate thermoresponsive polyelectrolyte multilayers (PEM) with the bioactive polyanion chondroitin sulfate (CS) at pH 4 by layer-by-layer technique. The in-situ investigation by surface plasmon resonance and quartz crystal microbalance with dissipation monitoring confirms that the formation of PEMs with CS can be achieved despite the bulky structure of PCHI at 25 °C. The stability of the PEMs is further improved at physiological pH 7.4 by chemical crosslinking using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide. Moreover, these PEMs exhibit de-swelling and swelling ability with different surface wettability in response to temperature, which triggers the adsorption and desorption of adhesive protein vitronectin on the PEMs. At 37 °C, the PEMs containing PNIPAM particularly associated with CS terminal layer supports protein adsorption and consequently enhances cell adhesion using multipotent murine stem cells. Overall, due to improved stability, crosslinked PNIPAM-modified biogenic multilayers are cytocompatible and hold great potential as culture substrate for different tissue cells and application in tissue engineering.
{"title":"Surface properties and bioactivity of PNIPAM-grafted-chitosan/chondroitin multilayers","authors":"Yi-Tung Lu , Pei-Tzu Hung , Kui Zeng , Christian Woelk , Bodo Fuhrmann , Kai Zhang , Thomas Groth","doi":"10.1016/j.smaim.2022.11.008","DOIUrl":"https://doi.org/10.1016/j.smaim.2022.11.008","url":null,"abstract":"<div><p>The thermoresponsive poly(<em>N</em>-isopropylacrylamide) (PNIPAM) is widely applied in the biomedical field particularly as thermoresponsive substrate for culture of cells. To be used as a stimuli-responsive coating for cell culture, combining PNIPAM with glycosaminoglycans might be an effective approach to improve its bioactivity. In this study, chitosan is grafted with PNIPAM moieties (PCHI) possessing a cloud point at 31 °C and used as a polycation to fabricate thermoresponsive polyelectrolyte multilayers (PEM) with the bioactive polyanion chondroitin sulfate (CS) at pH 4 by layer-by-layer technique. The <em>in-situ</em> investigation by surface plasmon resonance and quartz crystal microbalance with dissipation monitoring confirms that the formation of PEMs with CS can be achieved despite the bulky structure of PCHI at 25 °C. The stability of the PEMs is further improved at physiological pH 7.4 by chemical crosslinking using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/<em>N</em>-hydroxysuccinimide. Moreover, these PEMs exhibit de-swelling and swelling ability with different surface wettability in response to temperature, which triggers the adsorption and desorption of adhesive protein vitronectin on the PEMs. At 37 °C, the PEMs containing PNIPAM particularly associated with CS terminal layer supports protein adsorption and consequently enhances cell adhesion using multipotent murine stem cells. Overall, due to improved stability, crosslinked PNIPAM-modified biogenic multilayers are cytocompatible and hold great potential as culture substrate for different tissue cells and application in tissue engineering.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":"4 ","pages":"Pages 356-367"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49716991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1016/j.smaim.2023.06.004
Zheng Wang , Jiaxun Wang , Jiacheng Liu , Yating Zhang , Jingyi Zhang , Ruimeng Yang , Zhaosong Meng , Xiaoqun Gong , Lei Sui
The over-accumulation of ROS during prolonged in vitro expansion could negatively affect the properties of stem cells. This leads to a reduced capacity for self-renewal and a lower potential for multiple differentiation, ultimately hindering their applicability in regenerative medicine. Herein, we fabricated platinum nanoparticles (PtNPs) as a potential biocompatible antioxidant to efficiently eliminate the ROS accumulation in human dental follicle stem cells (hDFSCs) during in vitro expansion, thereby enhancing hDFSCs proliferation and osteogenic differentiation. Transcriptome analysis revealed that PI3K/AKT signaling pathway was activated in PtNPs-treated hDFSCs. Transplantation of PtNPs-treated rDFSCs could facilitate new bone formation compared to transplantation of PBS or un-treated rDFSCs, leading to efficient regeneration of bone tissue in rat mandibular bone defect models. In conclusion, PtNPs offered a novel antioxidative strategy to improve stem cell properties and stem-cells-based alveolar bone regeneration.
{"title":"Platinum nanoparticles enhance osteogenic differentiation of human dental follicle stem cells via scavenging ROS","authors":"Zheng Wang , Jiaxun Wang , Jiacheng Liu , Yating Zhang , Jingyi Zhang , Ruimeng Yang , Zhaosong Meng , Xiaoqun Gong , Lei Sui","doi":"10.1016/j.smaim.2023.06.004","DOIUrl":"https://doi.org/10.1016/j.smaim.2023.06.004","url":null,"abstract":"<div><p>The over-accumulation of ROS during prolonged <em>in vitro</em> expansion could negatively affect the properties of stem cells. This leads to a reduced capacity for self-renewal and a lower potential for multiple differentiation, ultimately hindering their applicability in regenerative medicine. Herein, we fabricated platinum nanoparticles (PtNPs) as a potential biocompatible antioxidant to efficiently eliminate the ROS accumulation in human dental follicle stem cells (hDFSCs) during <em>in vitro</em> expansion, thereby enhancing hDFSCs proliferation and osteogenic differentiation. Transcriptome analysis revealed that PI3K/AKT signaling pathway was activated in PtNPs-treated hDFSCs. Transplantation of PtNPs-treated rDFSCs could facilitate new bone formation compared to transplantation of PBS or un-treated rDFSCs, leading to efficient regeneration of bone tissue in rat mandibular bone defect models. In conclusion, PtNPs offered a novel antioxidative strategy to improve stem cell properties and stem-cells-based alveolar bone regeneration.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":"4 ","pages":"Pages 621-638"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49717150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1016/j.smaim.2023.01.001
Zhengchao Yuan , Lixiang Zhang , Shichao Jiang , Muhammad Shafiq , Youjun Cai , Yujie Chen , Jiahui Song , Xiao Yu , Hiroyuki Ijima , Yuan Xu , Xiumei Mo
Excessive scar tissue formation along with bacterial infection, hemorrhage, and oxidative wound microenvironment pose adverse physiological and psychological effects on patients, which necessitate the advent of innovative anti-inflammatory, anti-bacterial, and anti-oxidative multifunctional wound dressings. The overarching objective of this study was to exploit bioactive glass (BG) and a natural anti-bacterial component namely “oregano essential oil (OEO)” to impart multifunctionality to poly(L-lactide-co-glycolide)/Gelatin (PLGA/Gel)-based nanofibrous dressings for excisional wound management. We performed a series of structural, morphological, and release studies as well as delineated angiogenic, hemostatic, anti-bacterial, and anti-oxidative properties of these bioactive dressings in vitro, which altogether revealed the beneficial effects of BG and OEO in terms of rapid hemostasis, improved chemotactic response, diminished bacterial colonization, and anti-inflammatory response. Impressively, in multiple injury models, including a rat tail-amputation model, an ear artery injury model, and a liver trauma model in rabbit in vivo, we reported BG-mediated rapid hemostasis. Moreover, dressings containing BG showed improved hemocompatibility and suppressed coagulation as revealed by activated partial thromboplastin assay (APTT) in vitro. In addition, the transplantation of these nanofibrous dressings in a full-thickness excisional wound model in rats showed significant tissue regeneration as evidenced by the more number of blood vessels, glands, and hair follicles, re-epithelialization, diminished inflammatory response, and less fibrotic tissue formation. Taken together our approach of simultaneously harnessing economical BG and OEO to enable multifunctionality to nanofibrous dressings for tissue repair may hold great promise for wound healing as well as other bio-related disciplines.
{"title":"Anti-inflammatory, antibacterial, and antioxidative bioactive glass-based nanofibrous dressing enables scarless wound healing","authors":"Zhengchao Yuan , Lixiang Zhang , Shichao Jiang , Muhammad Shafiq , Youjun Cai , Yujie Chen , Jiahui Song , Xiao Yu , Hiroyuki Ijima , Yuan Xu , Xiumei Mo","doi":"10.1016/j.smaim.2023.01.001","DOIUrl":"https://doi.org/10.1016/j.smaim.2023.01.001","url":null,"abstract":"<div><p>Excessive scar tissue formation along with bacterial infection, hemorrhage, and oxidative wound microenvironment pose adverse physiological and psychological effects on patients, which necessitate the advent of innovative anti-inflammatory, anti-bacterial, and anti-oxidative multifunctional wound dressings. The overarching objective of this study was to exploit bioactive glass (BG) and a natural anti-bacterial component namely “oregano essential oil (OEO)” to impart multifunctionality to poly(L-lactide-co-glycolide)/Gelatin (PLGA/Gel)-based nanofibrous dressings for excisional wound management. We performed a series of structural, morphological, and release studies as well as delineated angiogenic, hemostatic, anti-bacterial, and anti-oxidative properties of these bioactive dressings <em>in vitro</em>, which altogether revealed the beneficial effects of BG and OEO in terms of rapid hemostasis, improved chemotactic response, diminished bacterial colonization, and anti-inflammatory response. Impressively, in multiple injury models, including a rat tail-amputation model, an ear artery injury model, and a liver trauma model in rabbit <em>in vivo</em>, we reported BG-mediated rapid hemostasis. Moreover, dressings containing BG showed improved hemocompatibility and suppressed coagulation as revealed by activated partial thromboplastin assay (APTT) <em>in vitro</em>. In addition, the transplantation of these nanofibrous dressings in a full-thickness excisional wound model in rats showed significant tissue regeneration as evidenced by the more number of blood vessels, glands, and hair follicles, re-epithelialization, diminished inflammatory response, and less fibrotic tissue formation. Taken together our approach of simultaneously harnessing economical BG and OEO to enable multifunctionality to nanofibrous dressings for tissue repair may hold great promise for wound healing as well as other bio-related disciplines.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":"4 ","pages":"Pages 407-426"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49734597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1016/j.smaim.2023.03.003
Chang Liu , Yun Wang , Pei Wang , Yan Gong , Bingcheng Yi , Jing Ruan , Xiansong Wang
Alleviating excessive inflammation while accelerating chronic wound healing to prevent wound infection has remained challenging, especially during the coronavirus disease 2019 (COVID-19) pandemic caused by SARS-CoV-2 when patients experienced difficulties with receive appropriate healthcare. We addressed this issue by developing handheld electrospun aloe-nanofiber membranes (ANFMs) with convenient, environmentally friendly properties and a therapeutic capacity for wound closure. Our results showed that ANFMs fabricated with high molecular weight polyvinyl alcohol (PVA) to form fibers during electrospinning had uniform fibrous architecture and a porous structure. Given the value of aloe gel in accelerating wound healing, liquid extracts from ANFMs significantly downregulated the expression of the pro-inflammatory genes, interleukin-6 (IL-6) and inducible nitric oxide synthase (iNOS), and markedly suppress the generation of reactive oxygen species (ROS) induced by lipopolysaccharide in RAW264.7 macrophages. These results indicated the excellent antioxidant and anti-inflammatory effects of ANFMs. After implantation into a mouse diabetic wound model for 12 days in situ, ANFMs notably expedited chronic wound healing via promoting angiogenesis and enhancing cell viability. Our ANFMs generated by handheld electrospinning in situ healed chronic wounds offer a convenient and promising alternative for patients to heal their own wounds under variable conditions.
{"title":"In situ electrospun aloe-nanofiber membrane for chronic wound healing","authors":"Chang Liu , Yun Wang , Pei Wang , Yan Gong , Bingcheng Yi , Jing Ruan , Xiansong Wang","doi":"10.1016/j.smaim.2023.03.003","DOIUrl":"https://doi.org/10.1016/j.smaim.2023.03.003","url":null,"abstract":"<div><p>Alleviating excessive inflammation while accelerating chronic wound healing to prevent wound infection has remained challenging, especially during the coronavirus disease 2019 (COVID-19) pandemic caused by SARS-CoV-2 when patients experienced difficulties with receive appropriate healthcare. We addressed this issue by developing handheld electrospun aloe-nanofiber membranes (ANFMs) with convenient, environmentally friendly properties and a therapeutic capacity for wound closure. Our results showed that ANFMs fabricated with high molecular weight polyvinyl alcohol (PVA) to form fibers during electrospinning had uniform fibrous architecture and a porous structure. Given the value of aloe gel in accelerating wound healing, liquid extracts from ANFMs significantly downregulated the expression of the pro-inflammatory genes, <em>interleukin-6</em> (<em>IL-6</em>) and <em>inducible nitric oxide synthase</em> (<em>iNOS</em>), and markedly suppress the generation of reactive oxygen species (ROS) induced by lipopolysaccharide in RAW264.7 macrophages. These results indicated the excellent antioxidant and anti-inflammatory effects of ANFMs. After implantation into a mouse diabetic wound model for 12 days <em>in situ</em>, ANFMs notably expedited chronic wound healing <em>via</em> promoting angiogenesis and enhancing cell viability. Our ANFMs generated by handheld electrospinning <em>in situ</em> healed chronic wounds offer a convenient and promising alternative for patients to heal their own wounds under variable conditions.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":"4 ","pages":"Pages 514-521"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49734611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1016/j.smaim.2022.08.002
Lamia Sami Mokeem , Isadora Martini Garcia , Yasmin Shahkarami , Lauren Blum , Abdulrahman A. Balhaddad , Fabrício Mezzomo Collares , Mary Ann Williams , Michael D. Weir , Mary Anne S. Melo
Dental resin adhesives and composites are the most prevailing dental restorative materials used to treat cavitated tooth decay. These materials are challenged inside the mouth by bacterial acid attack, lack of bioactivity, and the scarcity of alternatives maintaining the mechanical properties over the lifetime service of these materials. Core-shell nanostructures are composed of various materials surrounded by a protective shell. They are acquiring considerable attention as innovative multipurpose carriers that show great potential in restorative dentistry. Herein, we systematically reviewed the recent studies on core-shell nanostructures incorporated into dental resin-based materials, their intended properties, synthesis methods, and assessment tests employed. This study used scoping review method, following Arksey and O'Malley's five stages framework using PubMed and Scopus (Elsevier) databases. From 149 initially identified manuscripts, 20 studies were eligible for full-text screening, and 15 were included for data extraction. The majority of included studies have used resin composite as parental material. Silica oxide was the most prevailing shell incorporated into dental resins. Almost all core-shell nanostructures were added to improve the material's strength and impart antibacterial properties. Designing strategies and drug release behaviors were discussed. In the end, current challenges and prospects in this promising field were highlighted.
{"title":"Core-shell nanostructures for improving dental restorative materials: A scoping review of composition, methods, and outcome","authors":"Lamia Sami Mokeem , Isadora Martini Garcia , Yasmin Shahkarami , Lauren Blum , Abdulrahman A. Balhaddad , Fabrício Mezzomo Collares , Mary Ann Williams , Michael D. Weir , Mary Anne S. Melo","doi":"10.1016/j.smaim.2022.08.002","DOIUrl":"10.1016/j.smaim.2022.08.002","url":null,"abstract":"<div><p>Dental resin adhesives and composites are the most prevailing dental restorative materials used to treat cavitated tooth decay. These materials are challenged inside the mouth by bacterial acid attack, lack of bioactivity, and the scarcity of alternatives maintaining the mechanical properties over the lifetime service of these materials. Core-shell nanostructures are composed of various materials surrounded by a protective shell. They are acquiring considerable attention as innovative multipurpose carriers that show great potential in restorative dentistry. Herein, we systematically reviewed the recent studies on core-shell nanostructures incorporated into dental resin-based materials, their intended properties, synthesis methods, and assessment tests employed. This study used scoping review method, following Arksey and O'Malley's five stages framework using PubMed and Scopus (Elsevier) databases. From 149 initially identified manuscripts, 20 studies were eligible for full-text screening, and 15 were included for data extraction. The majority of included studies have used resin composite as parental material. Silica oxide was the most prevailing shell incorporated into dental resins. Almost all core-shell nanostructures were added to improve the material's strength and impart antibacterial properties. Designing strategies and drug release behaviors were discussed. In the end, current challenges and prospects in this promising field were highlighted.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":"4 ","pages":"Pages 102-110"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42278386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1016/j.smaim.2023.05.001
Zhenzhen Wu , Le Fan , Cuiting Chen , Yuanyuan Ma , Xiangnan Wu , Ying Li , Zhichao Hao , Tao Yang
The impaired osteogenic ability and excessive accumulation of reactive oxygen species (ROS) under osteoporosis severely weaken repair performance of biomimetic bone grafts. Currently, biomimetic bone grafts, capable of highly simulating bone hierarchy, could remarkably promote bone regeneration without systemic disease. Decorating biomimetic bone grafts with bioactivities without compromising hierarchical biomimicry stands as a feasible approach to treat the osteoporotic bone defect. Herein, through mineralizing decellularized collagen lamellae via strontium (Sr)- amorphous calcium phosphate and further modifying with tannic acid (TA), TA modified Sr-doped biomimetic bone lamellae was engineered. The physicochemical properties, ROS scavenging capacity and pro-osteogenic effect on osteoporotic bone marrow mesenchymal stem cells of construct were systemically evaluated. The results showed that TA and Sr can be successfully decorated without impairing the nano- and micro-architecture of biomimetic bone lamellae. The construct not only exhibited a potent and long-standing performance to eliminate ROS, but also effectively fostered the proliferation and osteogenic differentiation of osteoporotic bone marrow mesenchymal stem cells under oxidative stress environment. After implantation in the critical-sized bone defect of osteoporotic rat, it potently facilitated bone regeneration via synergistically activating PI3K/AKT signaling pathway. Hence, this construct is projected to be candidate for further engineering biomimetic bone grafts with more complicated hierarchy for accelerated healing of the osteoporotic bone defect.
{"title":"Promotion of osteoporotic bone healing by a tannic acid modified strontium-doped biomimetic bone lamella with ROS scavenging capacity and pro-osteogenic effect","authors":"Zhenzhen Wu , Le Fan , Cuiting Chen , Yuanyuan Ma , Xiangnan Wu , Ying Li , Zhichao Hao , Tao Yang","doi":"10.1016/j.smaim.2023.05.001","DOIUrl":"10.1016/j.smaim.2023.05.001","url":null,"abstract":"<div><p>The impaired osteogenic ability and excessive accumulation of reactive oxygen species (ROS) under osteoporosis severely weaken repair performance of biomimetic bone grafts. Currently, biomimetic bone grafts, capable of highly simulating bone hierarchy, could remarkably promote bone regeneration without systemic disease. Decorating biomimetic bone grafts with bioactivities without compromising hierarchical biomimicry stands as a feasible approach to treat the osteoporotic bone defect. Herein, through mineralizing decellularized collagen lamellae via strontium (Sr)- amorphous calcium phosphate and further modifying with tannic acid (TA), TA modified Sr-doped biomimetic bone lamellae was engineered. The physicochemical properties, ROS scavenging capacity and pro-osteogenic effect on osteoporotic bone marrow mesenchymal stem cells of construct were systemically evaluated. The results showed that TA and Sr can be successfully decorated without impairing the nano- and micro-architecture of biomimetic bone lamellae. The construct not only exhibited a potent and long-standing performance to eliminate ROS, but also effectively fostered the proliferation and osteogenic differentiation of osteoporotic bone marrow mesenchymal stem cells under oxidative stress environment. After implantation in the critical-sized bone defect of osteoporotic rat, it potently facilitated bone regeneration via synergistically activating PI3K/AKT signaling pathway. Hence, this construct is projected to be candidate for further engineering biomimetic bone grafts with more complicated hierarchy for accelerated healing of the osteoporotic bone defect.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":"4 ","pages":"Pages 590-602"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42345573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1016/j.smaim.2022.09.003
Yang Xiang , Qi-Quan Wang , Xin-Qiang Lan , Hui-Jie Zhang , Dai-Xu Wei
Metabolic intermediates serve as precursors for bioactive molecule synthesis, the energy source for life activities, and signals for environmental adaptation. Ketone bodies are important metabolic intermediates produced in the liver by the degradation of fatty acids, acting as an alternative energy source for extrahepatic tissues when glucose is short in supply (especially during starvation). β-hydroxybutyric acid, with its conjugate base β-hydroxybutyrate, constitutes approximately 70% of ketone bodies. A growing number of studies have demonstrated the beneficial effects of β-hydroxybutyrate, especially in delaying aging, intervening in aging-related disease, and promoting longevity. This review systematically reviews the role of β-hydroxybutyrate in aging hallmarks, shedding light on the possible molecular mechanism by which β-hydroxybutyrate supports healthy aging. Higher circulating β-hydroxybutyrate can be achieved by lifestyle modification (ketogenic diet or caloric restriction) or exogenous β-hydroxybutyrate (or β-hydroxybutyrate precursors, derivates and agonists) supplementation. We will also discuss the pros and cons of different ways to upregulate β-hydroxybutyrate, emphasizing the promising future clinical use of poly-β-hydroxybutyrate, the polymers of β-hydroxybutyrate, which can be easily produced via a microbial platform and synthetic biology.
{"title":"Function and treatment strategies of β-hydroxybutyrate in aging","authors":"Yang Xiang , Qi-Quan Wang , Xin-Qiang Lan , Hui-Jie Zhang , Dai-Xu Wei","doi":"10.1016/j.smaim.2022.09.003","DOIUrl":"10.1016/j.smaim.2022.09.003","url":null,"abstract":"<div><p>Metabolic intermediates serve as precursors for bioactive molecule synthesis, the energy source for life activities, and signals for environmental adaptation. Ketone bodies are important metabolic intermediates produced in the liver by the degradation of fatty acids, acting as an alternative energy source for extrahepatic tissues when glucose is short in supply (especially during starvation). β-hydroxybutyric acid, with its conjugate base β-hydroxybutyrate, constitutes approximately 70% of ketone bodies. A growing number of studies have demonstrated the beneficial effects of β-hydroxybutyrate, especially in delaying aging, intervening in aging-related disease, and promoting longevity. This review systematically reviews the role of β-hydroxybutyrate in aging hallmarks, shedding light on the possible molecular mechanism by which β-hydroxybutyrate supports healthy aging. Higher circulating β-hydroxybutyrate can be achieved by lifestyle modification (ketogenic diet or caloric restriction) or exogenous β-hydroxybutyrate (or β-hydroxybutyrate precursors, derivates and agonists) supplementation. We will also discuss the pros and cons of different ways to upregulate β-hydroxybutyrate, emphasizing the promising future clinical use of poly-β-hydroxybutyrate, the polymers of β-hydroxybutyrate, which can be easily produced via a microbial platform and synthetic biology.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":"4 ","pages":"Pages 160-172"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44281679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}