Enterococci are the most common pathogens in hospital acquired infections. Some of them are resistant to Vancomycin(VRE) and some are susceptible to Vancomycin (VSE). The present study was carried out to identify enterococci from clinical cases and to illustrate their clinical features and drug resistance characteristics. Antibiotics susceptibility of the identified bacteria was determined by disk diffusion method and E-test. Drug resistance properties were evaluated against ampicillin, gentamicin, vancomycin, teicoplanin and linezolid. Relating to clinical features, 244 cases of enterococci infected patients were identified at hospital Kuala Lumpur (HKL) Malaysia based on clinical information from the hospital. Of the patients 21% had history of urinary tract infections, 17.2% end stage renal disease, 12.2% sepsis, 8.4% malignancy, 12.2% had head injury and neurological problems, 4.2% diabetes mellitus and other clinical manifestations .In case of Vancomycin resistant enterococci infection, the clinical features of the patients were: end stage renal failure 3/6 (50%) and others with diabetes mellitus, interstitial lung disease and nephrotic syndrome. The patients of the enterococci infections were more prevalent in nephrology-urology unit (39%) and medical wards (including ICU, 23%) The findings would serve as an alert to the clinicians of the emergence of infections by enterococci and encourage implementation of appropriate infection control measures in order to curb further rise in prevalence.
{"title":"Enterococci: Emerging Drug Resistant Bacteria In Hospital Acquired Infections At Hospital Kuala Lumpur, Malaysia","authors":"R. Ibrahim, M. Mohamad, Mahmudur Rahman","doi":"10.5580/abb","DOIUrl":"https://doi.org/10.5580/abb","url":null,"abstract":"Enterococci are the most common pathogens in hospital acquired infections. Some of them are resistant to Vancomycin(VRE) and some are susceptible to Vancomycin (VSE). The present study was carried out to identify enterococci from clinical cases and to illustrate their clinical features and drug resistance characteristics. Antibiotics susceptibility of the identified bacteria was determined by disk diffusion method and E-test. Drug resistance properties were evaluated against ampicillin, gentamicin, vancomycin, teicoplanin and linezolid. Relating to clinical features, 244 cases of enterococci infected patients were identified at hospital Kuala Lumpur (HKL) Malaysia based on clinical information from the hospital. Of the patients 21% had history of urinary tract infections, 17.2% end stage renal disease, 12.2% sepsis, 8.4% malignancy, 12.2% had head injury and neurological problems, 4.2% diabetes mellitus and other clinical manifestations .In case of Vancomycin resistant enterococci infection, the clinical features of the patients were: end stage renal failure 3/6 (50%) and others with diabetes mellitus, interstitial lung disease and nephrotic syndrome. The patients of the enterococci infections were more prevalent in nephrology-urology unit (39%) and medical wards (including ICU, 23%) The findings would serve as an alert to the clinicians of the emergence of infections by enterococci and encourage implementation of appropriate infection control measures in order to curb further rise in prevalence.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78268161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Geeta Paul, R. Paul, M. Virdi, Priyank Banthia, Prerna Mathur
The challenge to copy nature and at the same time to maintain oral harmony is a complex theme in the field of anterior tooth restoration .The know how about the synthesis of form, function and superficial structure and the color finish of the natural teeth, together form the basis of aesthetic restorations. Metal free restorations have a strong ceramic core onto which layering ceramic is applied to achieve natural appearance. These have unique light optic relation feature that facilitates the reproduction of natural teeth .This article presents a case report in which teeth discolored due to tetracycline staining were aesthetically managed by all ceramic veneers.
{"title":"All Ceramic Veneers- A Case Reort","authors":"Geeta Paul, R. Paul, M. Virdi, Priyank Banthia, Prerna Mathur","doi":"10.5580/213","DOIUrl":"https://doi.org/10.5580/213","url":null,"abstract":"The challenge to copy nature and at the same time to maintain oral harmony is a complex theme in the field of anterior tooth restoration .The know how about the synthesis of form, function and superficial structure and the color finish of the natural teeth, together form the basis of aesthetic restorations. Metal free restorations have a strong ceramic core onto which layering ceramic is applied to achieve natural appearance. These have unique light optic relation feature that facilitates the reproduction of natural teeth .This article presents a case report in which teeth discolored due to tetracycline staining were aesthetically managed by all ceramic veneers.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86947230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Porcelain is known to be the most aesthetic material for dental restorations. It can imitate nature and at the same time maintain oral harmony which is a complex theme in the field of anterior tooth restoration. The knowhow about the synthesis, form, function and superficial structure and the color finish of the natural teeth, together form the basis of aesthetic restoration. A new generation of ceramic substance which distinguishes itself by unique light optic relation feature has facilitated the reproduction of natural teeth. The concept which has been outlined in this article informs about a systematic and rationale advance in the field of anterior tooth restoration while fulfilling highly aesthetic requirement. This case study presents the management of midline diastema using two different types of metal free ceramics
{"title":"Midline Diastema Closure Following Post-Orthodontic Treatment Relapse Using Metal Free Restorations","authors":"R. Paul, Geeta Paul, R. Paul, T. Juneja, M. Virdi","doi":"10.5580/23a0","DOIUrl":"https://doi.org/10.5580/23a0","url":null,"abstract":"Porcelain is known to be the most aesthetic material for dental restorations. It can imitate nature and at the same time maintain oral harmony which is a complex theme in the field of anterior tooth restoration. The knowhow about the synthesis, form, function and superficial structure and the color finish of the natural teeth, together form the basis of aesthetic restoration. A new generation of ceramic substance which distinguishes itself by unique light optic relation feature has facilitated the reproduction of natural teeth. The concept which has been outlined in this article informs about a systematic and rationale advance in the field of anterior tooth restoration while fulfilling highly aesthetic requirement. This case study presents the management of midline diastema using two different types of metal free ceramics","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87420184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdullah Alanazi, J. Alenazi, Suliman Alola, E. Banyan, M. Shalaan
Staphylococcus aureus (S. Aureus) is the most common species of staphylococci to cause Staph infections. The infections caused by S. aureus were not difficult to manage. However, in recent past, S. aureus is transformed to its variant forms, including, Methicillin-resistant S. aureus (MRSA) by acquiring methicillin resistant gene (mec A gene). Previously, the MRSA was known for its origin from hospitals, but more recently, it is known to be acquired, from the communities too. Here we report the case presentation of a male adolescent with complicated fibular osteomyelitis, necrotizing fasciitis and deep vein thrombosis caused by Community acquired (CA) MRSA. The preliminary complaint of the patient was swelling in right foot and leg associated with high fever, pain, dyspnea and cough. The clinical presentation fit him as acute respiratory distress syndrome and hence antibiotics (ceftixone and vancomycin) were started. Doppler ultrasound revealed deep vein thrombosis (DVT). Analysis of blood culture showed the presence of MRSA. Hence rifampin and gentamicin were added and ceftrixon was discontinued. MRI revealed ostiomyelitis in the distal part of fibula. The bone infection was so severe that despite of using the three antibiotics, the patient remained febrile with increased leukocytes and neutrophills, the ankle redness became confined with malleollus on each side and the sepsis remained uncontrollable. Repeated MRI showed inflammatory findings, including cellulitis, pyogenic myositis, deep fasciitis and fibular periosteal reactions, suggestive of necrotizing fasciitis. Upon confirmation of fasciitis, fasciotomy with debridement and irrigations was done in multiple sessions and a high dose intravenous immunoglobulin was administered as an adjuvant therapy, resulting in reversal of the disease progression. In light of reports in the literature, we replaced antibiotics with immunoglobulins as a post surgery treatment, which worked well.
{"title":"Community-Acquired Mecithillin-Resistant Staphylococcus Aureus : A Case Report on Necrotizing Fasciitis And Deep Vein Thrombosis In Adolescent Boy","authors":"Abdullah Alanazi, J. Alenazi, Suliman Alola, E. Banyan, M. Shalaan","doi":"10.5580/cc5","DOIUrl":"https://doi.org/10.5580/cc5","url":null,"abstract":"Staphylococcus aureus (S. Aureus) is the most common species of staphylococci to cause Staph infections. The infections caused by S. aureus were not difficult to manage. However, in recent past, S. aureus is transformed to its variant forms, including, Methicillin-resistant S. aureus (MRSA) by acquiring methicillin resistant gene (mec A gene). Previously, the MRSA was known for its origin from hospitals, but more recently, it is known to be acquired, from the communities too. Here we report the case presentation of a male adolescent with complicated fibular osteomyelitis, necrotizing fasciitis and deep vein thrombosis caused by Community acquired (CA) MRSA. The preliminary complaint of the patient was swelling in right foot and leg associated with high fever, pain, dyspnea and cough. The clinical presentation fit him as acute respiratory distress syndrome and hence antibiotics (ceftixone and vancomycin) were started. Doppler ultrasound revealed deep vein thrombosis (DVT). Analysis of blood culture showed the presence of MRSA. Hence rifampin and gentamicin were added and ceftrixon was discontinued. MRI revealed ostiomyelitis in the distal part of fibula. The bone infection was so severe that despite of using the three antibiotics, the patient remained febrile with increased leukocytes and neutrophills, the ankle redness became confined with malleollus on each side and the sepsis remained uncontrollable. Repeated MRI showed inflammatory findings, including cellulitis, pyogenic myositis, deep fasciitis and fibular periosteal reactions, suggestive of necrotizing fasciitis. Upon confirmation of fasciitis, fasciotomy with debridement and irrigations was done in multiple sessions and a high dose intravenous immunoglobulin was administered as an adjuvant therapy, resulting in reversal of the disease progression. In light of reports in the literature, we replaced antibiotics with immunoglobulins as a post surgery treatment, which worked well.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"269 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76988035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shailesh Kumar, S. Umadevi, N. Joseph, A. Kali, J. Easow, S. Srirangaraj, G. Kandhakumari, R. Singh, Pravin M. V. Charles, S. Stephen
Background: Inducible clindamycin resistance is a major concern for the use of clindamycin to treat staphylococcal infections. Aims: To determine the prevalence of inducible clindamycin resistance in clinical isolates of Staphylococcus spp. and the susceptibility pattern of the isolates. Materials and Methods: A total of 300 isolates of Staphylococci spp. recovered from different clinical specimens were studied. All the Staphylococcus spp. were identified by conventional microbiological methods. Inducible clindamycin resistance was detected by double disk approximation test (Dtest).Results: Of the 300 isolates, 176 were identified as S. aureus, while 124 were coagulase negative staphylococci (CoNS). The rates of inducible clindamycin resistance in methicillin resistant S. aureus (MRSA), methicillin sensitive S. aureus (MSSA), methicillin resistant CoNS (MR-CoNS) and methicillin sensitive CoNS (MSCoNS) were 75.0%, 24%, 18.8% and 11.1%, respectively. The inducible clindamycin resistance was significantly more among MRSA compared to methicillin sensitive S. aureus (MSSA) (P value < 0.0001). Majority of the MRSA isolates were susceptible to clindamycin, vancomycin and linezolid, while most of them were resistant to erythromycin, gentamicin, ciprofloxacin, tetracycline and sulfamethoxazole-trimethoprim. Conclusion: In view of the significant in vitro inducible clindamycin resistance in Staphylococcus spp., we recommend that D test should be used as a mandatory method in microbiology laboratories to avoid misinterpretation of clindamycin result.
{"title":"Detection of inducible clindamycin resistance in Staphylococcus aureus and coagulase-negative staphylococci - a study from South India","authors":"Shailesh Kumar, S. Umadevi, N. Joseph, A. Kali, J. Easow, S. Srirangaraj, G. Kandhakumari, R. Singh, Pravin M. V. Charles, S. Stephen","doi":"10.5580/1279","DOIUrl":"https://doi.org/10.5580/1279","url":null,"abstract":"Background: Inducible clindamycin resistance is a major concern for the use of clindamycin to treat staphylococcal infections. Aims: To determine the prevalence of inducible clindamycin resistance in clinical isolates of Staphylococcus spp. and the susceptibility pattern of the isolates. Materials and Methods: A total of 300 isolates of Staphylococci spp. recovered from different clinical specimens were studied. All the Staphylococcus spp. were identified by conventional microbiological methods. Inducible clindamycin resistance was detected by double disk approximation test (Dtest).Results: Of the 300 isolates, 176 were identified as S. aureus, while 124 were coagulase negative staphylococci (CoNS). The rates of inducible clindamycin resistance in methicillin resistant S. aureus (MRSA), methicillin sensitive S. aureus (MSSA), methicillin resistant CoNS (MR-CoNS) and methicillin sensitive CoNS (MSCoNS) were 75.0%, 24%, 18.8% and 11.1%, respectively. The inducible clindamycin resistance was significantly more among MRSA compared to methicillin sensitive S. aureus (MSSA) (P value < 0.0001). Majority of the MRSA isolates were susceptible to clindamycin, vancomycin and linezolid, while most of them were resistant to erythromycin, gentamicin, ciprofloxacin, tetracycline and sulfamethoxazole-trimethoprim. Conclusion: In view of the significant in vitro inducible clindamycin resistance in Staphylococcus spp., we recommend that D test should be used as a mandatory method in microbiology laboratories to avoid misinterpretation of clindamycin result.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81085997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Kathuria, Neelam Gupta, Manisha, R. Prasad, Nikita
Aloe vera is known for its many health benefits. It helps in improving the appearance of skin, treat digestive problems and boost immune systems. Use of aloe vera is beneficial in many oral conditions. Due to its anti bacterial qualities it is effective in fighting the bacteria and preventing bad breath, gingivitis, stomatitis and periodontitis. Acute mouth lesions are improved by direct application of aloe vera gel on herpetic viral lesions, apthous ulcers and cracks occurring at corner of mouth. Denture patients with sore spots and ill fitting complete or partial dentures can benefit by the use of aloe vera. It also promotes cell growth and act as detoxifying agent.
{"title":"Biologic Effects Of Aloe Vera Gel","authors":"N. Kathuria, Neelam Gupta, Manisha, R. Prasad, Nikita","doi":"10.5580/c3f","DOIUrl":"https://doi.org/10.5580/c3f","url":null,"abstract":"Aloe vera is known for its many health benefits. It helps in improving the appearance of skin, treat digestive problems and boost immune systems. Use of aloe vera is beneficial in many oral conditions. Due to its anti bacterial qualities it is effective in fighting the bacteria and preventing bad breath, gingivitis, stomatitis and periodontitis. Acute mouth lesions are improved by direct application of aloe vera gel on herpetic viral lesions, apthous ulcers and cracks occurring at corner of mouth. Denture patients with sore spots and ill fitting complete or partial dentures can benefit by the use of aloe vera. It also promotes cell growth and act as detoxifying agent.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90106609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was to observe clinical, serolgical and molecular diagnosis of chikungunya viral fever patients and its comparison with dengue viral fever. For that 49 serologically negative dengue patients but clinically dengue like symptomms were included. Clinical investigation was carried out recording different profiles of hospitalized patients. Serology was performed using the Onsite® Chikungunya IgM Combo rapid test and molecular test RT-PCR was performed to detect the virus in the patients sera. It was observed that out of them 19 (38.7 %) were serologically confirmed chikungunya infection. Interestingly molecular detection of the patients sera did not show the presence chikungunya virus but detected dengue virus from 9 patients’s sera. Classical clinical features of chikungunya virus infected patients were recorded to differentiate chikungunya from dengue, which were fever, arthralgia, myalgia and rash. Detection of chikungunya IgM in these cohort means that this virus is circulating throughout the year although not as many as dengue. Laboratory confirmation is important to differentiate chikungunya fever from dengue fever. This study adds some information in local data and hopefully can help the clinicians to clinically and laboratory diagnosis and management of chikungunya infection in outbreak and non-outbreak setting. Serology was proven to be useful in confirming chikungunya infection.
{"title":"Chikungunya confused with dengue in Malaysia: Clinical, serological and molecular perspective","authors":"Umi Kalsom Ali, I. Isahak, M. Rahman","doi":"10.5580/647","DOIUrl":"https://doi.org/10.5580/647","url":null,"abstract":"This study was to observe clinical, serolgical and molecular diagnosis of chikungunya viral fever patients and its comparison with dengue viral fever. For that 49 serologically negative dengue patients but clinically dengue like symptomms were included. Clinical investigation was carried out recording different profiles of hospitalized patients. Serology was performed using the Onsite® Chikungunya IgM Combo rapid test and molecular test RT-PCR was performed to detect the virus in the patients sera. It was observed that out of them 19 (38.7 %) were serologically confirmed chikungunya infection. Interestingly molecular detection of the patients sera did not show the presence chikungunya virus but detected dengue virus from 9 patients’s sera. Classical clinical features of chikungunya virus infected patients were recorded to differentiate chikungunya from dengue, which were fever, arthralgia, myalgia and rash. Detection of chikungunya IgM in these cohort means that this virus is circulating throughout the year although not as many as dengue. Laboratory confirmation is important to differentiate chikungunya fever from dengue fever. This study adds some information in local data and hopefully can help the clinicians to clinically and laboratory diagnosis and management of chikungunya infection in outbreak and non-outbreak setting. Serology was proven to be useful in confirming chikungunya infection.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88659614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A strain of Bacillus cereus, isolated from fermented cabbage, was tested a laboratory scale in order to evaluate the production of extracellular protease on batch cultivation in Modified Basal Medium without agar (MB). Furthermore optimization of culture medium and cultivation conditions were carried out too.B. cereus spp. presented a typical growth kinetic with a short lag phase. The best conditions for B. cereus spp. growth were found at pH 7, 32oC and in MB with glucose. Respecting to protease production, the best expression were at pH 8 and 32oC. At this temperature the addition of modifiers in the fermentation media did not produce a significant improvement in the protease production. The novel properties of the protease liberated by this strain could have important benefic for the biotechnology industry.
{"title":"Characterization of Bacillus cereus isolated from fermented cabbage and conventional optimization of extracellular protease production","authors":"C. E. Kotlar, Ponce A.G, R. Sansevero, S. Roura","doi":"10.5580/e7d","DOIUrl":"https://doi.org/10.5580/e7d","url":null,"abstract":"A strain of Bacillus cereus, isolated from fermented cabbage, was tested a laboratory scale in order to evaluate the production of extracellular protease on batch cultivation in Modified Basal Medium without agar (MB). Furthermore optimization of culture medium and cultivation conditions were carried out too.B. cereus spp. presented a typical growth kinetic with a short lag phase. The best conditions for B. cereus spp. growth were found at pH 7, 32oC and in MB with glucose. Respecting to protease production, the best expression were at pH 8 and 32oC. At this temperature the addition of modifiers in the fermentation media did not produce a significant improvement in the protease production. The novel properties of the protease liberated by this strain could have important benefic for the biotechnology industry.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2009-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75346340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Inocent C Omalu, V. Ayanwale, A. B. Ajalaruru, A. Z. Mohammed, J. Bala, V. Chukwuemeka
Housefly larvae were cultured on meat and collected for the isolation and identification of microorganisms associated with them. The microbes were cultured from both the gut and body surface of the maggot on nutrient agar (for bacteria) and potato dextrose agar (for fungi) and incubated at about 37°C for 48 hrs before observations. A variety of microorganisms, which includes the pathogenic Staphylococus aureus, pseudomonas sp. and Aspergillus niger and Aspergillus flavus were isolated.
{"title":"Isolation Of Fungi And Bacteria From Housefly (Musca Domestica L.) Larvae","authors":"Inocent C Omalu, V. Ayanwale, A. B. Ajalaruru, A. Z. Mohammed, J. Bala, V. Chukwuemeka","doi":"10.5580/47d","DOIUrl":"https://doi.org/10.5580/47d","url":null,"abstract":"Housefly larvae were cultured on meat and collected for the isolation and identification of microorganisms associated with them. The microbes were cultured from both the gut and body surface of the maggot on nutrient agar (for bacteria) and potato dextrose agar (for fungi) and incubated at about 37°C for 48 hrs before observations. A variety of microorganisms, which includes the pathogenic Staphylococus aureus, pseudomonas sp. and Aspergillus niger and Aspergillus flavus were isolated.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"144 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2009-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78536956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microorganisms producing keratinases are gaining prominence in recycling of poultry waste feather. Keratinase producing Bacillus sp. Isolated, were subjected to strain improvement along with optimisation of parameters of fermentation. Scaled up enzyme was partially purified and characterized to evaluate its application potential. Keratinase isolated from the bacillus species exhibited good stability with > 90% activity being retained for one month at refrigerated conditions and 50% activity was observed even after two months. At room temperature, the enzyme was active for 24 � 48 hours with optimum temperature of activity being 55∞C. The activity range was between 28∞C - 55∞C and pH optima was 8.5 which matched with optima for production of the enzyme. ~38kDa keratinase produced by the isolates was categorized into subtilisin group of serine proteinase based on the sensitivity to protease inhibitors, Keratinase from the test isolates was only marginally affected by surfactants, organic solvents. A significant enhancement in activity was observed in presence of reducing agents indicating possible versatile application potential for this enzyme with high stability
{"title":"Purification and Characterization of Keratinase fromFeather Degrading Bacillus sp.","authors":"Jeevana Lakshmi. Peddu, C. Chitturi, V. Lakshmi","doi":"10.5580/159b","DOIUrl":"https://doi.org/10.5580/159b","url":null,"abstract":"Microorganisms producing keratinases are gaining prominence in recycling of poultry waste feather. Keratinase producing Bacillus sp. Isolated, were subjected to strain improvement along with optimisation of parameters of fermentation. Scaled up enzyme was partially purified and characterized to evaluate its application potential. Keratinase isolated from the bacillus species exhibited good stability with > 90% activity being retained for one month at refrigerated conditions and 50% activity was observed even after two months. At room temperature, the enzyme was active for 24 � 48 hours with optimum temperature of activity being 55∞C. The activity range was between 28∞C - 55∞C and pH optima was 8.5 which matched with optima for production of the enzyme. ~38kDa keratinase produced by the isolates was categorized into subtilisin group of serine proteinase based on the sensitivity to protease inhibitors, Keratinase from the test isolates was only marginally affected by surfactants, organic solvents. A significant enhancement in activity was observed in presence of reducing agents indicating possible versatile application potential for this enzyme with high stability","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"376 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2009-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74876169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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