A total of 1200 drinking water samples from various water sources in Amritsar district, were analyzed to assess bacteriological quality of water. Presumptive coliform count by multiple tube test was done. The study revealed that 570 (47.5%) samples were unfit for human consumption. Of all the samples tested, 63/110(57.3%) samples from religious places, 28/56(50%) from hospitals, 25/50(50%) from bus stands/railway stations, 84/190(44.4%) from schools, 32/100(32%) from BSF checkposts, 5/19(26.5%) from hotels and 3/25(12%) from ice factories were found to be unsatisfactory. Out of the 570 unsatisfactory samples detected, 72% samples from submersible pumps,50% from hand pumps, 45.4% from taps,29.2% from tube wells and 28.5% from domestic tap supply were found to be contaminated. On Eijkman test 20/25(80%) of the suspicious samples turned out to be unsatisfactory. So, availability of safe drinking water still remains a much sought after commodity for majority of public in developing countries like India. Source of support: Govt. of Punjab (India)
{"title":"How safe is the safe water supply","authors":"S. Malhotra, U. Arora, P. Devi","doi":"10.5580/1","DOIUrl":"https://doi.org/10.5580/1","url":null,"abstract":"A total of 1200 drinking water samples from various water sources in Amritsar district, were analyzed to assess bacteriological quality of water. Presumptive coliform count by multiple tube test was done. The study revealed that 570 (47.5%) samples were unfit for human consumption. Of all the samples tested, 63/110(57.3%) samples from religious places, 28/56(50%) from hospitals, 25/50(50%) from bus stands/railway stations, 84/190(44.4%) from schools, 32/100(32%) from BSF checkposts, 5/19(26.5%) from hotels and 3/25(12%) from ice factories were found to be unsatisfactory. Out of the 570 unsatisfactory samples detected, 72% samples from submersible pumps,50% from hand pumps, 45.4% from taps,29.2% from tube wells and 28.5% from domestic tap supply were found to be contaminated. On Eijkman test 20/25(80%) of the suspicious samples turned out to be unsatisfactory. So, availability of safe drinking water still remains a much sought after commodity for majority of public in developing countries like India. Source of support: Govt. of Punjab (India)","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85093909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A Gram-positive, hexavalent chromium [chromate: Cr (VI)]-resistant & reducing bacterium, isolated from sukind chromite mines, jajpur, India, was identified as a Brevibacterium casei(Gene Bank Accession Number: EU781952) by gene sequence homology. The strain(designated as APD15) could tolerate chromium up to a maximum concentration of 500 ppm, at optimum temperature and pH 30C and 7 for maximum chromium reduction. Agar supplemented with 100g Cr (VI)/ml as K2Cr2O7 and 0.5% (w/v) dextrose used as a carbon source. The results of the study indicated removal of more than 94% chromium (VI) by Brevibacterium caseidetermined by diphenylcarbazide colorimetric assay.
{"title":"Hexavalent chromium reduction and 16S rDNA identification of bacteria isolated from a Cr (VI) contaminated site","authors":"A. Das, Susmita Mishra","doi":"10.5580/10ae","DOIUrl":"https://doi.org/10.5580/10ae","url":null,"abstract":"A Gram-positive, hexavalent chromium [chromate: Cr (VI)]-resistant & reducing bacterium, isolated from sukind chromite mines, jajpur, India, was identified as a Brevibacterium casei(Gene Bank Accession Number: EU781952) by gene sequence homology. The strain(designated as APD15) could tolerate chromium up to a maximum concentration of 500 ppm, at optimum temperature and pH 30C and 7 for maximum chromium reduction. Agar supplemented with 100g Cr (VI)/ml as K2Cr2O7 and 0.5% (w/v) dextrose used as a carbon source. The results of the study indicated removal of more than 94% chromium (VI) by Brevibacterium caseidetermined by diphenylcarbazide colorimetric assay.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"197 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79915999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effect of some metabolic inhibitors on citric acid production by Aspergillus niger in cane molasses medium was investigated. Addition of 0.01-0.1 iodoacetic acid and sodium arsenate, 0.05 -1.0 sodium malonate, 0.01 sodium azide , 0.01-0.05 sodium fluoride , 0.1-1.0 EDTA stimulated citric acid production (5-49%), but not necessary total titratable acids. Higher concentrations 10mM of iodoacetic acid , sodium malonate and 0.5 mM sodium azide caused a complete inhibition of fungal growth. 0.2 mM of iodoacetic acid, sodium arsenate and sodium fluoride caused a remarkable inhibition of citric acid production. The implications of those preliminary functions was discussed.
{"title":"Metabolic inhibitors as stimulating factors for citric acid production","authors":"Z. Nehad, M. Essam, A. Hába","doi":"10.5580/1946","DOIUrl":"https://doi.org/10.5580/1946","url":null,"abstract":"The effect of some metabolic inhibitors on citric acid production by Aspergillus niger in cane molasses medium was investigated. Addition of 0.01-0.1 iodoacetic acid and sodium arsenate, 0.05 -1.0 sodium malonate, 0.01 sodium azide , 0.01-0.05 sodium fluoride , 0.1-1.0 EDTA stimulated citric acid production (5-49%), but not necessary total titratable acids. Higher concentrations 10mM of iodoacetic acid , sodium malonate and 0.5 mM sodium azide caused a complete inhibition of fungal growth. 0.2 mM of iodoacetic acid, sodium arsenate and sodium fluoride caused a remarkable inhibition of citric acid production. The implications of those preliminary functions was discussed.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"104 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88097963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Wadhwani, Komal Desai, Dhara Patel, Deepmala Lawani, Priyadarshani Bahaley, P. Joshi, V. Kothari
Dimethyl sulfoxide (DMSO) and ethanol are frequently used as solvent for natural as well as synthetic antibacterial compounds, in order to determine their MICs. Effect of these solvents on bacterial growth is an important factor to be considered, while considering reproducibility of experiments for MIC determination. Present study aimed at determining the effect of different concentrations (1% to 6%) of DMSO, ethanol, and methanol on the growth of five different bacteria. DMSO scored better followed by methanol and ethanol, in terms of their compatibility with MIC determination. Lower concentrations of solvents, which apparently do not affect the bacterial growth significantly, may still potentiate the effect of antibacterial compound under test.
{"title":"Effect of various solvents on bacterial growth in context of determining MIC of various antimicrobials","authors":"T. Wadhwani, Komal Desai, Dhara Patel, Deepmala Lawani, Priyadarshani Bahaley, P. Joshi, V. Kothari","doi":"10.5580/b43","DOIUrl":"https://doi.org/10.5580/b43","url":null,"abstract":"Dimethyl sulfoxide (DMSO) and ethanol are frequently used as solvent for natural as well as synthetic antibacterial compounds, in order to determine their MICs. Effect of these solvents on bacterial growth is an important factor to be considered, while considering reproducibility of experiments for MIC determination. Present study aimed at determining the effect of different concentrations (1% to 6%) of DMSO, ethanol, and methanol on the growth of five different bacteria. DMSO scored better followed by methanol and ethanol, in terms of their compatibility with MIC determination. Lower concentrations of solvents, which apparently do not affect the bacterial growth significantly, may still potentiate the effect of antibacterial compound under test.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86118446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant protection is an important area which needs attention since most of the hazardous inputs added into the agricultural system are in the form of plant protection chemicals. Pseudomonas fluorescens possess a variety of promising properties which make it a better biocontrol agent. The objectives of the present study were to isolate P.fluorescens from soil, to check its antagonistic activity and effect of its secondary metabolites on three fungal plant pathogens by in vitro techniques. P.fluorscens was isolated from rhizosphere soil on King’s B medium and its antagonistic effect on three fungal plant pathogens was studied in vitro. Its antagonistic activity was checked by co-inoculation with the fungal isolates. In pour plate method, P.fluorescens on coinoculation with fugal pathogens decreased their growth rate. Maximum inhibition was observed in Pythium ultimim (80%) followed by Macrophomina phaseolina (70%) and Pyricularia oryzae (50%). Effect of the separated secondary metabolites on the fungal growth by broth dilution technique and antifungal activity by agar well diffusion technique was studied. P.fluorescens produces a broad-spectrum antifungal compound, which inhibits a variety of plant Pathogenic fungi and inhibits Pythium ultimim more when compared to other plant pathogens in the present study. Further investigations on the type of antifungal components and in vivo experiments will make P.fluorescens as one of the most suitable biocontrol agent in suppressing the phytopathogenic fungi and replace chemical fungicides.
{"title":"Biological control of three phytopathogenic fungi by Pseudomonas fluorescens isolated from rhizosphere.","authors":"M. Goud, V. Muralikrishnan","doi":"10.5580/c13","DOIUrl":"https://doi.org/10.5580/c13","url":null,"abstract":"Plant protection is an important area which needs attention since most of the hazardous inputs added into the agricultural system are in the form of plant protection chemicals. Pseudomonas fluorescens possess a variety of promising properties which make it a better biocontrol agent. The objectives of the present study were to isolate P.fluorescens from soil, to check its antagonistic activity and effect of its secondary metabolites on three fungal plant pathogens by in vitro techniques. P.fluorscens was isolated from rhizosphere soil on King’s B medium and its antagonistic effect on three fungal plant pathogens was studied in vitro. Its antagonistic activity was checked by co-inoculation with the fungal isolates. In pour plate method, P.fluorescens on coinoculation with fugal pathogens decreased their growth rate. Maximum inhibition was observed in Pythium ultimim (80%) followed by Macrophomina phaseolina (70%) and Pyricularia oryzae (50%). Effect of the separated secondary metabolites on the fungal growth by broth dilution technique and antifungal activity by agar well diffusion technique was studied. P.fluorescens produces a broad-spectrum antifungal compound, which inhibits a variety of plant Pathogenic fungi and inhibits Pythium ultimim more when compared to other plant pathogens in the present study. Further investigations on the type of antifungal components and in vivo experiments will make P.fluorescens as one of the most suitable biocontrol agent in suppressing the phytopathogenic fungi and replace chemical fungicides.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79213079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: Medicinal plants are an important therapeutic aid for various ailments. The invitro antimicrobial activity of Albizzia lebbeck. Benth was screened against some pathogens isolated from diarrhoeal patients.Procedure: The bark of the plant was powdered and extracted separately with aqueous, methanol, chloroform, petroleum ether and hexane using maceration technique. The antimicrobial activity was carried out by disc diffusion method and minimum inhibitory concentration by two-fold serial dilution method. The microorganisms tested include Enterotoxigenic E.coli, Enteropathogenic E.coli, Salmonella typhimurium, Salmonella enteritidis, Shigella dysenteria, Shigella flexineri, Candida albicans, Candida tropicalis and Candida krusei. Amikacin 10 μg/disc and fluconazole 10 μg/disc were used as standards.R esults: Aqueous, methanol and chloroform extracts exhibited activity against E.coli and Salmonella species. Petroleum ether and hexane extracts did not exhibit any activity. None of the extracts showed activity against Shigella and Candida species activity.C onclusion: This study revealed that the methanol extracts of Albizzia lebbeck was effective against E.coli and Salmonella strains associated with infectious diarrhoea. Further isolation of active compound responsible for the activity could be the potential sources of new antimicrobial agents
{"title":"Antimicrobial Activity of Albizzia Lebbeck Benth against Infectious Diarrhoea","authors":"B. Uma, K. Prabhakar, S. Rajendran, Y. L. Sarayu","doi":"10.5580/c7","DOIUrl":"https://doi.org/10.5580/c7","url":null,"abstract":"Aim: Medicinal plants are an important therapeutic aid for various ailments. The invitro antimicrobial activity of Albizzia lebbeck. Benth was screened against some pathogens isolated from diarrhoeal patients.Procedure: The bark of the plant was powdered and extracted separately with aqueous, methanol, chloroform, petroleum ether and hexane using maceration technique. The antimicrobial activity was carried out by disc diffusion method and minimum inhibitory concentration by two-fold serial dilution method. The microorganisms tested include Enterotoxigenic E.coli, Enteropathogenic E.coli, Salmonella typhimurium, Salmonella enteritidis, Shigella dysenteria, Shigella flexineri, Candida albicans, Candida tropicalis and Candida krusei. Amikacin 10 μg/disc and fluconazole 10 μg/disc were used as standards.R esults: Aqueous, methanol and chloroform extracts exhibited activity against E.coli and Salmonella species. Petroleum ether and hexane extracts did not exhibit any activity. None of the extracts showed activity against Shigella and Candida species activity.C onclusion: This study revealed that the methanol extracts of Albizzia lebbeck was effective against E.coli and Salmonella strains associated with infectious diarrhoea. Further isolation of active compound responsible for the activity could be the potential sources of new antimicrobial agents","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79906753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The diversity of Aspergillus from soil of Kusmi Forest, an Indian subtropical Teak forest at Gorakhpur resulted in characterizing a total of 415 isolates which were then subjected to amylases production. The preliminary amylases production revealed a total of 14 promising isolates of Aspergillus comprising of ten different strains of A. flavus, two strains of A. tamarii, one strain each of A. niger and A. awamori which are characterized further. The optimum temperature of amylases produced from 14 isolates ranged from 30 to 60 C while thermal stability observed was in the range of 50 to 80 C for 30 minutes. The pH optima ranged from 5.0 to 8.6 and the Km values calculated ranged from 0.60 to 2.85 mg/ml.
{"title":"Isolation and screening of different isolates of Aspergillus for amylases production","authors":"V. Morya, D. Yadav","doi":"10.5580/21f8","DOIUrl":"https://doi.org/10.5580/21f8","url":null,"abstract":"The diversity of Aspergillus from soil of Kusmi Forest, an Indian subtropical Teak forest at Gorakhpur resulted in characterizing a total of 415 isolates which were then subjected to amylases production. The preliminary amylases production revealed a total of 14 promising isolates of Aspergillus comprising of ten different strains of A. flavus, two strains of A. tamarii, one strain each of A. niger and A. awamori which are characterized further. The optimum temperature of amylases produced from 14 isolates ranged from 30 to 60 C while thermal stability observed was in the range of 50 to 80 C for 30 minutes. The pH optima ranged from 5.0 to 8.6 and the Km values calculated ranged from 0.60 to 2.85 mg/ml.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80132321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Chandraleka, S. Basha, G. Chandramohan, A. Panneerselvam, D. Dhanasekaran
The study report the synthesis of the Cu(III), with Isonicotinic acid hydrazide and 2-acetylnaphthalene and their characterization using FTIR and Magnetic susceptibility studies. Our studies reveal the presence of different mode of linkages of the ligand with Cu(III), The comparison of the IR spectra of the ligand imply that the ligand is bidentate with the amide group and azomethine –nitrogen as the two coordination sites. The complexes exhibit an identical pattern suggesting them to be isostructural with six coordinated spin free octahedral complexes. The complexes have been screened for their antibacterial and antifungal activity. The results of this study shows that the Cu(III), complex is effective against fungal pathogen than the bacterial pathogens.
{"title":"Synthesis and antimicrobial activity of Cu (III) schiff base complex","authors":"S. Chandraleka, S. Basha, G. Chandramohan, A. Panneerselvam, D. Dhanasekaran","doi":"10.5580/1076","DOIUrl":"https://doi.org/10.5580/1076","url":null,"abstract":"The study report the synthesis of the Cu(III), with Isonicotinic acid hydrazide and 2-acetylnaphthalene and their characterization using FTIR and Magnetic susceptibility studies. Our studies reveal the presence of different mode of linkages of the ligand with Cu(III), The comparison of the IR spectra of the ligand imply that the ligand is bidentate with the amide group and azomethine –nitrogen as the two coordination sites. The complexes exhibit an identical pattern suggesting them to be isostructural with six coordinated spin free octahedral complexes. The complexes have been screened for their antibacterial and antifungal activity. The results of this study shows that the Cu(III), complex is effective against fungal pathogen than the bacterial pathogens.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77933855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Growth of Aspergillus niger on Czapek-dox medium supplemented with native lignocellulosics like Sawdust, wheat straw, Sugarcane baggage and Ricebran used for the production of cellulase by A.niger. Of these Czapek-dox medium with 0.5% Wheat straw had yielded more cellulase, FPase (2.9 U/ml), CMCase (2.9 U/ml) and β-glucosidase (0.93 U/ml) after 14-days of incubation. The lignocellulosics after pre-treatment improved cellulase production. Among the treated substrates, Czapek-dox medium with 0.5% saw dust had yielded FPase (6.3 U/ml), CMCase (7.2 U/ml) after 7-days of incubation and the medium with treated rice bran produced β-glucosidase of 1.04 U/ml after 21-days of incubation.
{"title":"Production of Cellulase by Aspergillus niger on natural and pretreated lignocellulosic wastes","authors":"A. Sridevi, G. Narasimha, B. R. Reddy","doi":"10.5580/1884","DOIUrl":"https://doi.org/10.5580/1884","url":null,"abstract":"Growth of Aspergillus niger on Czapek-dox medium supplemented with native lignocellulosics like Sawdust, wheat straw, Sugarcane baggage and Ricebran used for the production of cellulase by A.niger. Of these Czapek-dox medium with 0.5% Wheat straw had yielded more cellulase, FPase (2.9 U/ml), CMCase (2.9 U/ml) and β-glucosidase (0.93 U/ml) after 14-days of incubation. The lignocellulosics after pre-treatment improved cellulase production. Among the treated substrates, Czapek-dox medium with 0.5% saw dust had yielded FPase (6.3 U/ml), CMCase (7.2 U/ml) after 7-days of incubation and the medium with treated rice bran produced β-glucosidase of 1.04 U/ml after 21-days of incubation.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78058883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To promote the overall quality of fermented dairy products (FDP) which depends on the types and characteristics of the starter organisms used in the production, the effect of medium composition on biomass growth, viscosity, exopolysaccharide and lactic acid production by EPSproducing lactic acid bacteria isolates was investigated. Medium composition had profound effect on the studied parameters. Among the five medium used for the cultivation of the isolates, maximum biomass growth and viscosity production was achieved in partially de-protenised whey medium (PDW) in which Lactococcus piscium (OLHF6) and Lactobacillus plantarum (LPN1) exhibit the highest biomass growth and viscosity with concentration of 1.58 and 1.561mPa.S. Lactobacillus plantarum (LPN1) (1.58mPa.S), L.casei (LCN1) (1.475 mPa.S), L.plantarum (LPN3) (1.48mPa.S), L.plantarum (LPY80 (1.48mPa.S) and Lactococcus piscium (OLHF6) (1.185mPa.S) was found to be a good starter for development of viscous medium in the respective media. Whey was the best medium for EPS production in which L. casei (LCN1)) was the best starter (198.69mg/l). Sweet whey medium, modified MRS medium, Whey and partially deproteinised whey medium was not favourable for lactic acid production by the isolates but Semidefine medium was the best for lactic acid development in which L.plantarum (LPW10) was found to be the best starter for lactic acid development (5.57g/l).
{"title":"Comparative influence of medium composition on biomass growth, lactic acid and Exopolysaccharides Production by some Strains of Lactic Acid Bacteria","authors":"B. Adebayo-Tayo, A. Onilude","doi":"10.5580/1d3d","DOIUrl":"https://doi.org/10.5580/1d3d","url":null,"abstract":"To promote the overall quality of fermented dairy products (FDP) which depends on the types and characteristics of the starter organisms used in the production, the effect of medium composition on biomass growth, viscosity, exopolysaccharide and lactic acid production by EPSproducing lactic acid bacteria isolates was investigated. Medium composition had profound effect on the studied parameters. Among the five medium used for the cultivation of the isolates, maximum biomass growth and viscosity production was achieved in partially de-protenised whey medium (PDW) in which Lactococcus piscium (OLHF6) and Lactobacillus plantarum (LPN1) exhibit the highest biomass growth and viscosity with concentration of 1.58 and 1.561mPa.S. Lactobacillus plantarum (LPN1) (1.58mPa.S), L.casei (LCN1) (1.475 mPa.S), L.plantarum (LPN3) (1.48mPa.S), L.plantarum (LPY80 (1.48mPa.S) and Lactococcus piscium (OLHF6) (1.185mPa.S) was found to be a good starter for development of viscous medium in the respective media. Whey was the best medium for EPS production in which L. casei (LCN1)) was the best starter (198.69mg/l). Sweet whey medium, modified MRS medium, Whey and partially deproteinised whey medium was not favourable for lactic acid production by the isolates but Semidefine medium was the best for lactic acid development in which L.plantarum (LPW10) was found to be the best starter for lactic acid development (5.57g/l).","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86331800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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