K. Nishikawa, Y. Furuta, Toshihiko Yamada, S. Kudo
In order to investigate the phylogeny of polyploid wheat, the genetic variation of α-amylase isozymes in 127 strains of two diploid wheat species and five Aegilops species belonging to section Sitopsis was analyzed. Three types of zymogram were detected in 39 strains of Triticum monococcum and three other different types were detected in another diploid wheat, T. urartu. Two varieties of Ae. speltoides, var. ligustica and var. aucheri (subsection Truncata), were similar to each other and showed wide variation of zymograms. Sixteen strains of Ae. longissima and 13 strains of Ae. sharonensis were each grouped into four types. Ten strains of Ae. bicornis were grouped into three types, each including three or four strains. On the other hand, zymograms from five strains of Ae. searsii, which was formerly treated as a variety of Ae. longissima, were very different from those of Ae. longissima. In contrast to subsection Truncata, the zymogram types were rather simple and similar among species in subsection Emarginata (Ae. longissima, Ae. sharonensis, and Ae.bicornis) except for Ae. searsii. Finally, the phylogeny of tetraploid wheat was discussed on the basis of the results presented here along with those of the previous authors.
为了研究多倍体小麦的系统发育，对隶属于Sitopsis科的2个二倍体小麦种和5个Aegilops种的127个品系α-淀粉酶同工酶的遗传变异进行了分析。在39株单粒小麦中检测到3种不同类型的酶谱，在另一株二倍体小麦T. urartu中检测到3种不同类型的酶谱。Ae的两个变种。speltoides, var. ligustica和var. aucheri (Truncata)的酶谱差异很大。16株伊蚊。13株伊蚊;Sharonensis可分为4种类型。10株伊蚊。双角鸟分为三种类型，每种类型包括三到四株。另一方面，5个菌株的酶谱分析表明;以前被认为是伊蚊的变种。与伊蚊有很大的不同。对发生。与Truncata分段相比，Emarginata分段的酶谱类型较为简单且相似。对,Ae。沙龙伊蚊和双角伊蚊)。searsii。最后，在本文和前人研究结果的基础上，对小麦的系统发育进行了讨论。
{"title":"Genetic studies of α-amylase isozymes in wheat VII. Variation in diploid ancestral species and phylogeny of tetraploid wheat","authors":"K. Nishikawa, Y. Furuta, Toshihiko Yamada, S. Kudo","doi":"10.1266/JJG.67.1","DOIUrl":"https://doi.org/10.1266/JJG.67.1","url":null,"abstract":"In order to investigate the phylogeny of polyploid wheat, the genetic variation of α-amylase isozymes in 127 strains of two diploid wheat species and five Aegilops species belonging to section Sitopsis was analyzed. Three types of zymogram were detected in 39 strains of Triticum monococcum and three other different types were detected in another diploid wheat, T. urartu. Two varieties of Ae. speltoides, var. ligustica and var. aucheri (subsection Truncata), were similar to each other and showed wide variation of zymograms. Sixteen strains of Ae. longissima and 13 strains of Ae. sharonensis were each grouped into four types. Ten strains of Ae. bicornis were grouped into three types, each including three or four strains. On the other hand, zymograms from five strains of Ae. searsii, which was formerly treated as a variety of Ae. longissima, were very different from those of Ae. longissima. In contrast to subsection Truncata, the zymogram types were rather simple and similar among species in subsection Emarginata (Ae. longissima, Ae. sharonensis, and Ae.bicornis) except for Ae. searsii. Finally, the phylogeny of tetraploid wheat was discussed on the basis of the results presented here along with those of the previous authors.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"24 1","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"1992-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76760559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Yoshimura, A. Yoshimura, A. Saito, N. Kishimoto, M. Kawase, M. Yano, M. Nakagahra, T. Ogawa, N. Iwata
Introgressed chromosome segments in three near-isogenic lines (NILs) of rice for bacterial blight resistance genes Xa-1, Xa-3 and Xa-4 with the genetic backgrounds of an IR24 variety were measured by using restriction fragment length polymorphic (RFLP) markers and linkage map. The size of the introgressed segment flanking Xa-1 of a NIL was estimated to be 3.3-11.7 cM of map units. Both NILs for Xa-3 and Xa-4 contained the donor segments at an identical end of linkage group corresponding to chromosome 11. We demonstrated in this study that these NILs were good materials to identify DNA markers tightly linked to these resistance genes and the RFLP markers tagging the resistance genes were useful in genetics and breeding on the disease resistance.
{"title":"RFLP analysis of introgressed chromosomal segments in three near-isogenic lines of rice for bacterial blight resistance genes, Xa-1, Xa-3 and Xa-4","authors":"S. Yoshimura, A. Yoshimura, A. Saito, N. Kishimoto, M. Kawase, M. Yano, M. Nakagahra, T. Ogawa, N. Iwata","doi":"10.1266/JJG.67.29","DOIUrl":"https://doi.org/10.1266/JJG.67.29","url":null,"abstract":"Introgressed chromosome segments in three near-isogenic lines (NILs) of rice for bacterial blight resistance genes Xa-1, Xa-3 and Xa-4 with the genetic backgrounds of an IR24 variety were measured by using restriction fragment length polymorphic (RFLP) markers and linkage map. The size of the introgressed segment flanking Xa-1 of a NIL was estimated to be 3.3-11.7 cM of map units. Both NILs for Xa-3 and Xa-4 contained the donor segments at an identical end of linkage group corresponding to chromosome 11. We demonstrated in this study that these NILs were good materials to identify DNA markers tightly linked to these resistance genes and the RFLP markers tagging the resistance genes were useful in genetics and breeding on the disease resistance.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"2 1","pages":"29-37"},"PeriodicalIF":0.0,"publicationDate":"1992-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90139835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In order to clarify the effect of selfing on genetic variation in geographically structured populations, Wright's island model is generalized to include; the effects of extinction, mutation, selfing, and migration of pollen and seed. The equilibrium probabilities of identity by descent for two genes are obtained. It is found that selfing causes a decrease of the variation within colonies and an increase of the variation between colonies. Our model partly explains the "heterozygosity paradox" but other factors such as selection are necessary to fully account for the paradox.
{"title":"Genetic variability and geographical structure in partially selfing populations","authors":"K. Maruyama, H. Tachida","doi":"10.1266/JJG.67.39","DOIUrl":"https://doi.org/10.1266/JJG.67.39","url":null,"abstract":"In order to clarify the effect of selfing on genetic variation in geographically structured populations, Wright's island model is generalized to include; the effects of extinction, mutation, selfing, and migration of pollen and seed. The equilibrium probabilities of identity by descent for two genes are obtained. It is found that selfing causes a decrease of the variation within colonies and an increase of the variation between colonies. Our model partly explains the \"heterozygosity paradox\" but other factors such as selection are necessary to fully account for the paradox.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"12 1","pages":"39-51"},"PeriodicalIF":0.0,"publicationDate":"1992-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74910106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The spatial distribution of allozyme alleles was studied at two local sites of a natural population of wild radish, an inland site in 1988 and 1989 and a beach site in 1988. Randomness of the spatial distribution of genotypes was tested at four isozyme loci in adult plants and fertilizing pollen by spatial autocorrelation statistics. At the inland site, significant aggregation of an identical allele was observed at two loci each in 1988 and 1989 for adult plants. At these loci, aggregation of an allele was observed also for pollen cloud. The aggregation, however, was not stable; the loci which showed significant aggregation differed in two successive seasons. These observations can be interpreted either by limited but moderate pollen dispersal or the great variation in fertility among the adult plants. The latter accidentally causes aggregation of identical alleles. At the beach site, the seed bank was disturbed by tractors for fishing work, and this led to a random distribution of alleles.
{"title":"Spatial autocorrelation analysis of allozyme variants within local sites of wild radish population","authors":"T. Tokunaga, O. Ohnishi","doi":"10.1266/JJG.67.209","DOIUrl":"https://doi.org/10.1266/JJG.67.209","url":null,"abstract":"The spatial distribution of allozyme alleles was studied at two local sites of a natural population of wild radish, an inland site in 1988 and 1989 and a beach site in 1988. Randomness of the spatial distribution of genotypes was tested at four isozyme loci in adult plants and fertilizing pollen by spatial autocorrelation statistics. At the inland site, significant aggregation of an identical allele was observed at two loci each in 1988 and 1989 for adult plants. At these loci, aggregation of an allele was observed also for pollen cloud. The aggregation, however, was not stable; the loci which showed significant aggregation differed in two successive seasons. These observations can be interpreted either by limited but moderate pollen dispersal or the great variation in fertility among the adult plants. The latter accidentally causes aggregation of identical alleles. At the beach site, the seed bank was disturbed by tractors for fishing work, and this led to a random distribution of alleles.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"1 1","pages":"209-216"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75327134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Inheritance of the flower color in chrysanthemum particularly anthocyanin pigmentation was analyzed by using spectrophotometry. Genetic analysis of the inheritance of anthocyanin pigmentation showed that chrysanthemum cultivars used in this experiment belonged to three groups. In the first group the gene for anthocyanin pigment biosynthesis appeared in the homozygous recessive state. The cultivars belonging to this group had either white or yellow flower and can be useful for testing lines for anthocyanin pigmentation. In the second group, the genes were in the heterozygous condition. While in the third group, the presence of two genes in the heterozygous state with two distinct genes was noted. It was suggested that a chimerical structure be considered in the analysis of the flower color inheritance. The possible role of the gene in the step involving dihydroflavonol to anthocyanin formation in the anthocyanin biosynthesis was also presented.
{"title":"Inheritance of anthocyanin pigmentation in flower color of chrysanthemum","authors":"K. Hattori","doi":"10.1266/JJG.67.253","DOIUrl":"https://doi.org/10.1266/JJG.67.253","url":null,"abstract":"Inheritance of the flower color in chrysanthemum particularly anthocyanin pigmentation was analyzed by using spectrophotometry. Genetic analysis of the inheritance of anthocyanin pigmentation showed that chrysanthemum cultivars used in this experiment belonged to three groups. In the first group the gene for anthocyanin pigment biosynthesis appeared in the homozygous recessive state. The cultivars belonging to this group had either white or yellow flower and can be useful for testing lines for anthocyanin pigmentation. In the second group, the genes were in the heterozygous condition. While in the third group, the presence of two genes in the heterozygous state with two distinct genes was noted. It was suggested that a chimerical structure be considered in the analysis of the flower color inheritance. The possible role of the gene in the step involving dihydroflavonol to anthocyanin formation in the anthocyanin biosynthesis was also presented.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"72 1","pages":"253-258"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87244885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tohru Suzuki, C. Nakamura, H. Sakagami, N. Mori, I. Panayotov, C. Kaneda
Alloplasmic lines of common wheat (Triticum aestivum L. cultivars `Penjamo 62' and `Siete Cerros 66') with cytoplasms of two wheatgrass species (Agropyron glaucum and Ag. trichophorum) showed severe growth depression throughout their development and male sterility at maturity. Growth vigor of the alloplasmic lines was influenced more by cytoplasm donors than by nucleus donors, while male sterility was influenced more by nucleus donors. Short-arm telocentric, nucleolus-organizing chromosomes (telosomes) derived from Agropyron caused the restoration of growth vigor and male fertility of the alloplasmic lines in which cytoplasms and telosomes from the same Agropyron species were combined. The telosomes derived from Agropyron species different from the cytoplasm donors could also recover growth vigor and male fertility of the alloplasmic lines. The result indicates the presence of a common mechanism(s) which controls the nucleus-cytoplasmic incompatibility expressed in the alloplasmic lines with the two Agropyron cytoplasms. The Agropyron telosomes showed some dosage effects on growth vigor of the alloplasmic lines and affected fertility when added to the nuclear backgrounds of the two common wheat cultivars.
{"title":"Interaction between telocentric nucleolus-organizing chromosomes of two Agropyron species and their cytoplasms in alloplasmic lines of common wheat","authors":"Tohru Suzuki, C. Nakamura, H. Sakagami, N. Mori, I. Panayotov, C. Kaneda","doi":"10.1266/JJG.67.133","DOIUrl":"https://doi.org/10.1266/JJG.67.133","url":null,"abstract":"Alloplasmic lines of common wheat (Triticum aestivum L. cultivars `Penjamo 62' and `Siete Cerros 66') with cytoplasms of two wheatgrass species (Agropyron glaucum and Ag. trichophorum) showed severe growth depression throughout their development and male sterility at maturity. Growth vigor of the alloplasmic lines was influenced more by cytoplasm donors than by nucleus donors, while male sterility was influenced more by nucleus donors. Short-arm telocentric, nucleolus-organizing chromosomes (telosomes) derived from Agropyron caused the restoration of growth vigor and male fertility of the alloplasmic lines in which cytoplasms and telosomes from the same Agropyron species were combined. The telosomes derived from Agropyron species different from the cytoplasm donors could also recover growth vigor and male fertility of the alloplasmic lines. The result indicates the presence of a common mechanism(s) which controls the nucleus-cytoplasmic incompatibility expressed in the alloplasmic lines with the two Agropyron cytoplasms. The Agropyron telosomes showed some dosage effects on growth vigor of the alloplasmic lines and affected fertility when added to the nuclear backgrounds of the two common wheat cultivars.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"37 1","pages":"133-145"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85827363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The ribosomal RNA (rRNA) genes were mapped on somatic chromosomes of Japanese red pine (Pinus densiflora) and Japanese black pine (P. thunbergii) by in situ hybridization using biotin-labeled 18S-25S rDNA. Cytochemical detection of the hybridized probe showed many signals in the interphase nuclei and, prophase and metaphase chromosomes in both species. In the metaphase complement of P. densiflora, the signals appeared singly at the interstitial region of seven pairs of the chromosomes. In P. thunbergii, ten strong and two weak signals appeared at the interstitial region of six pairs of the chromosomes. The hybridization sites coincided with the interstitial chromomycin A3-bands or secondary constricted regions.
{"title":"Multiple locations of the rRNA genes in chromosomes of pines, Pinus densiflora and P. thunbergii","authors":"M. Hizume, F. Ishida, M. Murata","doi":"10.1266/JJG.67.389","DOIUrl":"https://doi.org/10.1266/JJG.67.389","url":null,"abstract":"The ribosomal RNA (rRNA) genes were mapped on somatic chromosomes of Japanese red pine (Pinus densiflora) and Japanese black pine (P. thunbergii) by in situ hybridization using biotin-labeled 18S-25S rDNA. Cytochemical detection of the hybridized probe showed many signals in the interphase nuclei and, prophase and metaphase chromosomes in both species. In the metaphase complement of P. densiflora, the signals appeared singly at the interstitial region of seven pairs of the chromosomes. In P. thunbergii, ten strong and two weak signals appeared at the interstitial region of six pairs of the chromosomes. The hybridization sites coincided with the interstitial chromomycin A3-bands or secondary constricted regions.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"112 1","pages":"389-396"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73702576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Somatic chromosomes of 307 seedlings of Pseudotsuga menziesii (Mirb.) Franco var. menziesii from four native localities in Canada and 203 seedlings of P. menziesii var. glauca (Beissn) Franco from two localities in U.S.A. were stained with guanine-specific chromomycin A3 (CMA). The chromosome complements (2n=26) of the two varieties were commonly composed of ten metacentric, 12 submetacentric and four telocentric chromosomes. Six thick CMA-bands appeared in the interstitial region of a pair of metacentric chromosomes and in the proximal regions of the long arms of two pairs of submetacentric chromosomes. However, the CMA-bands varied in size among the members of the populations. The large CMA-bands of the submetacentric chromosome were observed more frequently in var. glauca than in var. menziesii. Frequency of each sized CMA-band varied within the populations or the varieties. Bright CMA-blocks were associated to nucleoli in the interphase nucleus and the CMA-bands were coincided in location with the secondary constrictions. Ribosomal DNA hybridized in situ to the thick CMA-band regions.
307株孟氏伪杉苗的体细胞染色体分析用鸟苷特异性色霉素A3 (CMA)对来自加拿大4个地方的门齐弧菌(Franco var. menziesii)和来自美国2个地方的门齐弧菌(P. menziesii var. glauca (Beissn) Franco) 203株幼苗进行了染色。两个品种的染色体互补体(2n=26)通常由10条常著心染色体、12条亚常著心染色体和4条远著心染色体组成。6条较厚的cma条带出现在一对稳心染色体的间隙区和两对亚稳心染色体的长臂近端。然而，种群成员的cma波段大小不同。在变种青光眼中观察到的大cma染色体带比变种孟氏型更常见。各cma波段的频率在种群或品种中存在差异。明亮的cma条带与间期核核仁相关，cma条带与二次缩窄的位置一致。核糖体DNA原位杂交到厚cma带区域。
{"title":"Size variation of chromomycin A3-band in chromosomes of Douglas fir, Pseudotsuga menziesii","authors":"M. Hizume, M. Akiyama","doi":"10.1266/JJG.67.425","DOIUrl":"https://doi.org/10.1266/JJG.67.425","url":null,"abstract":"Somatic chromosomes of 307 seedlings of Pseudotsuga menziesii (Mirb.) Franco var. menziesii from four native localities in Canada and 203 seedlings of P. menziesii var. glauca (Beissn) Franco from two localities in U.S.A. were stained with guanine-specific chromomycin A3 (CMA). The chromosome complements (2n=26) of the two varieties were commonly composed of ten metacentric, 12 submetacentric and four telocentric chromosomes. Six thick CMA-bands appeared in the interstitial region of a pair of metacentric chromosomes and in the proximal regions of the long arms of two pairs of submetacentric chromosomes. However, the CMA-bands varied in size among the members of the populations. The large CMA-bands of the submetacentric chromosome were observed more frequently in var. glauca than in var. menziesii. Frequency of each sized CMA-band varied within the populations or the varieties. Bright CMA-blocks were associated to nucleoli in the interphase nucleus and the CMA-bands were coincided in location with the secondary constrictions. Ribosomal DNA hybridized in situ to the thick CMA-band regions.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"59 1","pages":"425-435"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85504409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rice (Oryza sativa L. var. Nipponbare) seeds, seedlings and suspension callus were subjected to powerful gravity stress ranging from 150,000 g to 450,000 g. Suspension callus and dehulled seeds showed 32% and 15% survival rates, respectively, after gravity treatment at 450,000 g for 6 hours, whereas all the seedlings died. Seedlings became increasingly sensitive to gravity stress as the growth stage advanced. The suspension callus, which gradually recovered viability during subculture under normal conditions, were used to analyze the protein patterns and in vitro translation products of poly(A)+RNA. Some 3 to 4 newly synthesized proteins and in vitro translation products were identified using two-dimensional electrophoresis, while 5 to 24 proteins disappeared during the treatment, demonstrating that rice suspension callus respond to gravity stress by producing new mRNA and their proteins. In addition, some newly synthesized proteins which resulted from gravity effects were maintained in the surviving cells during continuous growth on solid medium. There was an increase in the survival rate of suspension callus from the strong gravity stress as a result of treatment with 200 g/l sucrose, 10-5 M ABA or 5 g/l NaCl. This suggests that in addition to changes in protein patterns, another protection mechanism associated with some physiological changes is involved in the survival of rice suspension callus after gravity stress.
水稻(Oryza sativa L. var. Nipponbare)种子、幼苗和悬浮愈伤组织均受到15万~ 45万g的强重力胁迫。45万g重力处理6 h后，悬浮愈伤组织和脱壳种子的存活率分别为32%和15%，幼苗全部死亡。随着生育期的推进，幼苗对重力胁迫的敏感性逐渐增强。在正常条件下继代培养逐渐恢复活力的悬浮愈伤组织，用于分析poly(A)+RNA的蛋白模式和体外翻译产物。通过双向电泳鉴定出3 ~ 4个新合成的蛋白和体外翻译产物，5 ~ 24个蛋白在处理过程中消失，说明水稻悬浮愈伤组织通过产生新的mRNA及其蛋白来响应重力胁迫。此外，存活细胞在固体培养基上连续生长过程中，由于重力效应而产生的一些新合成的蛋白质得以维持。200 g/l蔗糖、10-5 M ABA和5 g/l NaCl处理均能提高悬浮性愈伤组织在强重力胁迫下的存活率。这表明除了蛋白质模式的变化外，水稻悬浮愈伤组织在重力胁迫下的存活还涉及另一种与生理变化相关的保护机制。
{"title":"Gravity responsible protein and mRNA related to the survival of rice (Oryza sativa L.) from gravity stress","authors":"Soon-tae Kwon, K. Oono","doi":"10.1266/JJG.67.321","DOIUrl":"https://doi.org/10.1266/JJG.67.321","url":null,"abstract":"Rice (Oryza sativa L. var. Nipponbare) seeds, seedlings and suspension callus were subjected to powerful gravity stress ranging from 150,000 g to 450,000 g. Suspension callus and dehulled seeds showed 32% and 15% survival rates, respectively, after gravity treatment at 450,000 g for 6 hours, whereas all the seedlings died. Seedlings became increasingly sensitive to gravity stress as the growth stage advanced. The suspension callus, which gradually recovered viability during subculture under normal conditions, were used to analyze the protein patterns and in vitro translation products of poly(A)+RNA. Some 3 to 4 newly synthesized proteins and in vitro translation products were identified using two-dimensional electrophoresis, while 5 to 24 proteins disappeared during the treatment, demonstrating that rice suspension callus respond to gravity stress by producing new mRNA and their proteins. In addition, some newly synthesized proteins which resulted from gravity effects were maintained in the surviving cells during continuous growth on solid medium. There was an increase in the survival rate of suspension callus from the strong gravity stress as a result of treatment with 200 g/l sucrose, 10-5 M ABA or 5 g/l NaCl. This suggests that in addition to changes in protein patterns, another protection mechanism associated with some physiological changes is involved in the survival of rice suspension callus after gravity stress.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"1 1","pages":"321-334"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90238353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kanazawa, W. Sakamoto, M. Nakagahra, K. Kadowaki, N. Tsutsumi, S. Tano
Distribution of four kinds of mitochondrial plasmid-like DNAs, Bl, B2, B3 and B4 was investigated by Southern analysis using 85 accessions of cultivated rice (Oryza sativa L.). The frequencies of these molecules found in cultivars differed from each other. The mitochondrial genome were categorized into nine types according to their presence or absence. They were found mostly in ecospecies Indica, a few in Javanica, and not found in Japonica. The result indicated the polymorphic and monomorphic patterns of the mitochondrial genomic organization within Indica and Japonica cultivars, respectively. Quantitative variation was found among four kinds of mitochondiral plasmid-like DNAs, suggesting that these molecules were unequally distributed or replicated during the process of mitochondrial division.
{"title":"Distribution and quantitative variation of mitochondrial plasmid-like DNAs in cultivated rice (Oryza sativa L.)","authors":"A. Kanazawa, W. Sakamoto, M. Nakagahra, K. Kadowaki, N. Tsutsumi, S. Tano","doi":"10.1266/JJG.67.309","DOIUrl":"https://doi.org/10.1266/JJG.67.309","url":null,"abstract":"Distribution of four kinds of mitochondrial plasmid-like DNAs, Bl, B2, B3 and B4 was investigated by Southern analysis using 85 accessions of cultivated rice (Oryza sativa L.). The frequencies of these molecules found in cultivars differed from each other. The mitochondrial genome were categorized into nine types according to their presence or absence. They were found mostly in ecospecies Indica, a few in Javanica, and not found in Japonica. The result indicated the polymorphic and monomorphic patterns of the mitochondrial genomic organization within Indica and Japonica cultivars, respectively. Quantitative variation was found among four kinds of mitochondiral plasmid-like DNAs, suggesting that these molecules were unequally distributed or replicated during the process of mitochondrial division.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"7 1","pages":"309-319"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86314816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：