Pub Date : 2022-06-29DOI: 10.30651/jmlt.v5i1.10656
Yauwan Tobing Lukiyono, Vella Rohmayani
ABSTRACT Hydroponics is a technique for cultivating vegetable and fruit plants. The principle of hydroponic technique is the provision of nutrient solution as a source of plant nutrients. These plants are grown in certain media using water instead of soil. The presence of stagnant water in hydroponic techniques can be a breeding place for Aedes aegypti larvae. Aedes aegypti mosquito can do as a vector for dengue fever. Dengue fever is still a public health problem today. The purpose of this study was to analyze the potential of AB mix as a nutrient for hydroponic plants as larvicides of the Aedes aegypti mosquito.This research was a pure experimental research. The research design used a pre post only group design. The research sample was F3 larvae of Aedes aegypti instar III. The research variables were the mortality of Aedes aegypti larvae and variations in the concentration of AB mix. The test was repeated 10 times. Data were analyzed using one-way ANOVA. Based on the results obtained in all treatments there was death or mortality of mosquito larvae. The administration of 75% AB Mix with a duration of 70 minutes is the most optimal treatment to eradicate Aedes aegypti mosquito larvae. So, it can be concluded that AB Mix can be used as an alternative larvicide to eradicate Aedes aegypti mosquito larvae. Keywords : AB Mix, mortalitas dan Larva Aedes aegypti
{"title":"Efektifitas Nutrisi Hidroponik AB MIX Sebagai Larvasida Alternatif Larva Nyamuk Aedes aegypti","authors":"Yauwan Tobing Lukiyono, Vella Rohmayani","doi":"10.30651/jmlt.v5i1.10656","DOIUrl":"https://doi.org/10.30651/jmlt.v5i1.10656","url":null,"abstract":"ABSTRACT Hydroponics is a technique for cultivating vegetable and fruit plants. The principle of hydroponic technique is the provision of nutrient solution as a source of plant nutrients. These plants are grown in certain media using water instead of soil. The presence of stagnant water in hydroponic techniques can be a breeding place for Aedes aegypti larvae. Aedes aegypti mosquito can do as a vector for dengue fever. Dengue fever is still a public health problem today. The purpose of this study was to analyze the potential of AB mix as a nutrient for hydroponic plants as larvicides of the Aedes aegypti mosquito.This research was a pure experimental research. The research design used a pre post only group design. The research sample was F3 larvae of Aedes aegypti instar III. The research variables were the mortality of Aedes aegypti larvae and variations in the concentration of AB mix. The test was repeated 10 times. Data were analyzed using one-way ANOVA. Based on the results obtained in all treatments there was death or mortality of mosquito larvae. The administration of 75% AB Mix with a duration of 70 minutes is the most optimal treatment to eradicate Aedes aegypti mosquito larvae. So, it can be concluded that AB Mix can be used as an alternative larvicide to eradicate Aedes aegypti mosquito larvae. Keywords : AB Mix, mortalitas dan Larva Aedes aegypti","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76097761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-24DOI: 10.30651/jmlt.v5i1.12824
R. Widyastuti, Nur Vita Purwaningsih, Ellies Tunjung Sari Maulidiyanti, Tri Ade Saputro
The screening blood test is a stage that is carried out by each PMI to ensure the safety before blood transfusion. It is usually done on antibodies (Ab) such as anti-HCV, anti-HIV, TPHA or antigen (Ag) such as HBsAg. HBsAg is the outer coat protein of hepatitis B virus (HBV) and a sign that the individual has been infected with the hepatitis B virus. Hepatitis B virus can cause acute problems and even cirrhosis of the liver. One of the screening blood test methods which is used to detect hepatitis B is ELISA (Enzyme Linked Immuno Sorbent Assay) method. This research was conducted at UTD PMI Sampang by using the data collection in February 2020, and aimed to find out the overview of HBsAg examination results on the screening blood test using ELISA method based on age and sex. Based on the screening blood test, 9 people (3%) were reactive HBsAg and 345 people (97%) were non-reactive. In the age category, the highest number of reactive HBsAg donors was in the age group of 18-24 years old, that was 4 donors (1.1%), while the highest number of non-reactive HBsAg donors was in the age group of 25-44 years old, that was 184 people ( 51.9%). In the gender category, donors with the most reactive HBsAg results were male, that was 8 people (2.3%) and donors with the highest non-reactive HBsAg results was male, that was 283 people (79.9%). In order to minimize hepatitis B, it is expected that society should avoid activities which can cause hepatitis B infection. Keywords : HBsAg, Blood Transfusion, Screening Blood Test
{"title":"Prevalensi Hasil Uji Saring Hbsag Pada Darah Donor Di Unit Tranfusi Darah (UTD) PMI Sampang Madura","authors":"R. Widyastuti, Nur Vita Purwaningsih, Ellies Tunjung Sari Maulidiyanti, Tri Ade Saputro","doi":"10.30651/jmlt.v5i1.12824","DOIUrl":"https://doi.org/10.30651/jmlt.v5i1.12824","url":null,"abstract":"The screening blood test is a stage that is carried out by each PMI to ensure the safety before blood transfusion. It is usually done on antibodies (Ab) such as anti-HCV, anti-HIV, TPHA or antigen (Ag) such as HBsAg. HBsAg is the outer coat protein of hepatitis B virus (HBV) and a sign that the individual has been infected with the hepatitis B virus. Hepatitis B virus can cause acute problems and even cirrhosis of the liver. One of the screening blood test methods which is used to detect hepatitis B is ELISA (Enzyme Linked Immuno Sorbent Assay) method. This research was conducted at UTD PMI Sampang by using the data collection in February 2020, and aimed to find out the overview of HBsAg examination results on the screening blood test using ELISA method based on age and sex. Based on the screening blood test, 9 people (3%) were reactive HBsAg and 345 people (97%) were non-reactive. In the age category, the highest number of reactive HBsAg donors was in the age group of 18-24 years old, that was 4 donors (1.1%), while the highest number of non-reactive HBsAg donors was in the age group of 25-44 years old, that was 184 people ( 51.9%). In the gender category, donors with the most reactive HBsAg results were male, that was 8 people (2.3%) and donors with the highest non-reactive HBsAg results was male, that was 283 people (79.9%). In order to minimize hepatitis B, it is expected that society should avoid activities which can cause hepatitis B infection. Keywords : HBsAg, Blood Transfusion, Screening Blood Test ","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88504940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-21DOI: 10.30651/jmlt.v5i1.18048
Wieke Sriwulan
Free radicals are compounds that are very dangerous for the body because they can attack and cause cell damage in the body, therefore antioxidant compounds are needed that can counteract the presence of these free radicals. One of the fruits that contain antioxidant compounds is dates (Phoenix dactylifera L.) which contain flavonoid compounds that act as antioxidants. The purpose of this study was to determine the antioxidant activity at heating temperatures of 400C, 500C, and 650C. The extraction method used in this study is the maceration method with 80% ethanol as solvent. Testing of antioxidant activity using the method of reducing free radicals, namely the ABTS method and using vitamin C as a comparison control to show antioxidant activity. ABTS is a method of determining antioxidant activity obtained from the oxidation of potassium persulfate with diammonium salt. Absorbance measurements were carried out using a UV-Vis spectrophotometer with a wavelength of 520 nm. Data analysis was carried out using linear regression equations to determine the IC50 value in the sample of dates (Phoenix dactylifera L.). The IC50 value indicates the antioxidant activity in a sample, namely the smaller the IC50 value, the higher the antioxidant in a sample. The results of data analysis using the Kruskal Wallis test obtained a significance value of 0.104 which indicates that > 0.05 which means there is no significant difference between each treatment group. From the results of the analysis data, it is known that there is no effect of heating temperature on the antioxidant stability of dates, this is also strengthened by the value of antioxidant activity in the linear regression equation where the value of antioxidant activity in each treatment group has an equally strong antioxidant activity value. based on the Blois classification. Keywords : Phoenix dactylifera L, Antioxidant and Extract
{"title":"Stabilitas Antioksidan Buah Kurma (Phoenix dactylifera L.) Pada Suhu Pemanasan Dengan Metobe ABTS","authors":"Wieke Sriwulan","doi":"10.30651/jmlt.v5i1.18048","DOIUrl":"https://doi.org/10.30651/jmlt.v5i1.18048","url":null,"abstract":"Free radicals are compounds that are very dangerous for the body because they can attack and cause cell damage in the body, therefore antioxidant compounds are needed that can counteract the presence of these free radicals. One of the fruits that contain antioxidant compounds is dates (Phoenix dactylifera L.) which contain flavonoid compounds that act as antioxidants. The purpose of this study was to determine the antioxidant activity at heating temperatures of 400C, 500C, and 650C. The extraction method used in this study is the maceration method with 80% ethanol as solvent. Testing of antioxidant activity using the method of reducing free radicals, namely the ABTS method and using vitamin C as a comparison control to show antioxidant activity. ABTS is a method of determining antioxidant activity obtained from the oxidation of potassium persulfate with diammonium salt. Absorbance measurements were carried out using a UV-Vis spectrophotometer with a wavelength of 520 nm. Data analysis was carried out using linear regression equations to determine the IC50 value in the sample of dates (Phoenix dactylifera L.). The IC50 value indicates the antioxidant activity in a sample, namely the smaller the IC50 value, the higher the antioxidant in a sample. The results of data analysis using the Kruskal Wallis test obtained a significance value of 0.104 which indicates that > 0.05 which means there is no significant difference between each treatment group. From the results of the analysis data, it is known that there is no effect of heating temperature on the antioxidant stability of dates, this is also strengthened by the value of antioxidant activity in the linear regression equation where the value of antioxidant activity in each treatment group has an equally strong antioxidant activity value. based on the Blois classification. Keywords : Phoenix dactylifera L, Antioxidant and Extract ","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"106 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79287664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-30DOI: 10.30651/jmlt.v5i1.14604
Ellies Tunjung Sari Maulidiyanti, Fitrotin Azizah, Tri Ade Saputro, Firdausi Nuzula
Activated Partial Thromboplastine Time (APTT) is a type of hemostasis examination with citrate anticoagulant 3.2% ratio 1:9. Ethylene Diamine Tetraacetic Acid (EDTA) is one of the examination materials for hematology which is often used as an anticoagulant. Apart from EDTA, other types of anticoagulants are sodium citrate and potassium oxalate. The three anticoagulants work to bind calcium ions. The purpose of this examination is to determine the use of various anticoagulants on the quality of the results of the Activated Partial Thromboplastine Time (APCT) examination. The method used in this study is an experimental study with 3 sample groups, each consisting of 9 samples in one group. The SPSS results showed a probability value of 0.000 with the Anova test, the average results of the APTT examination were administration of sodium citrate anticoagulant 25.9 seconds, potassium oxalate 70.92 seconds, and EDTA 37.5 seconds. Based on the results of these studies, there are significant differences related to the use of various anticoagulants on the quality of the results of the Activated Partial Thromboplastine Time (APTT) examination.Keywords : Activated Partial Thromboplastine Time, Antikoagulans,
活化部分凝质体时间(Activated Thromboplastine Time, APTT)是一种以柠檬酸盐抗凝3.2%比1:9进行的止血检查。乙二胺四乙酸(EDTA)是血液学检测材料之一，常被用作抗凝血剂。除了EDTA，其他类型的抗凝剂还有柠檬酸钠和草酸钾。这三种抗凝剂的作用是结合钙离子。本检查的目的是确定使用各种抗凝剂对活化部分凝质体时间(APCT)检查结果的质量。本研究采用的方法是实验研究，有3个样本组，每组9个样本。SPSS统计分析的方差分析结果显示概率值为0.000,APTT检查的平均结果为枸橼酸钠抗凝剂25.9秒，草酸钾70.92秒，EDTA 37.5秒。基于这些研究的结果，不同抗凝剂的使用对活化部分凝质体时间(APTT)检测结果的质量有显著差异。关键词:活化部分凝质体时间;抗凝血酶;
{"title":"Penggunaan Berbagai Jenis Antikoagulan Terhadap Mutu Hasil Pemeriksaan Activated Partial Thromboplastine Time (APTT)","authors":"Ellies Tunjung Sari Maulidiyanti, Fitrotin Azizah, Tri Ade Saputro, Firdausi Nuzula","doi":"10.30651/jmlt.v5i1.14604","DOIUrl":"https://doi.org/10.30651/jmlt.v5i1.14604","url":null,"abstract":"Activated Partial Thromboplastine Time (APTT) is a type of hemostasis examination with citrate anticoagulant 3.2% ratio 1:9. Ethylene Diamine Tetraacetic Acid (EDTA) is one of the examination materials for hematology which is often used as an anticoagulant. Apart from EDTA, other types of anticoagulants are sodium citrate and potassium oxalate. The three anticoagulants work to bind calcium ions. The purpose of this examination is to determine the use of various anticoagulants on the quality of the results of the Activated Partial Thromboplastine Time (APCT) examination. The method used in this study is an experimental study with 3 sample groups, each consisting of 9 samples in one group. The SPSS results showed a probability value of 0.000 with the Anova test, the average results of the APTT examination were administration of sodium citrate anticoagulant 25.9 seconds, potassium oxalate 70.92 seconds, and EDTA 37.5 seconds. Based on the results of these studies, there are significant differences related to the use of various anticoagulants on the quality of the results of the Activated Partial Thromboplastine Time (APTT) examination.Keywords : Activated Partial Thromboplastine Time, Antikoagulans, ","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87477714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Staphylococcus aureus bacteria are common flora bacteria that are both commensal and opportunistic pathogens in humans. They are commonly treated with antibiotics, which can lead to bacterial resistance, such as the emergence of Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria, which are resistant to the β -lactam antibiotics that are currently widespread to a variety of communities, such as the campus community, which is equipped with several facilities such as laboratories. The microbiology laboratory is a practicum and research laboratory that uses materials in the form of microorganisms, hence it has the potential to transmit MRSA bacteria. The aim of this study is to find out if MRSA bacteria are present on microbiology lab equipment and how prevalent they are. This study used observational research with a descriptive research design and a purposive sampling strategy. The results of bacterial isolation on MSA medium from fourteen laboratory equipment revealed that thirteen devices exhibited bacterial growth, whereas one device had none. Seven bacterial isolates with S. aureus-like features are detected growing in three of the thirteen equipment, the LAF, incubator, and manual autoclave. The seven bacterial isolates were identified as having round colony shape, glistening, opaque, convex yellow pigmentation, round cell morphology, clustered like grapes and purple in color, positive for coagulase and catalase, and resistance to methicillin antibiotics (MRSA bacteria). It can be concluded that of the fourteen-laboratory equipment examined, three instruments, namely the LAF, incubator, and manual autoclave, were found to be overgrown with MRSA bacteria, with the prevalence of MRSA bacteria in microbiology laboratory equipment reaching 21.4%. Keywords: laboratory equipment, MRSA, prevalence, S. aureus
{"title":"Prevalensi Bakteri Methicillin-Resistant Staphylococcus aureus (MRSA) Pada Peralatan Laboratorium","authors":"Kurniawan Kurniawan -, Eva Adaning Tyas, S. .","doi":"10.30651/jmlt.v4i2.7554","DOIUrl":"https://doi.org/10.30651/jmlt.v4i2.7554","url":null,"abstract":"Staphylococcus aureus bacteria are common flora bacteria that are both commensal and opportunistic pathogens in humans. They are commonly treated with antibiotics, which can lead to bacterial resistance, such as the emergence of Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria, which are resistant to the β -lactam antibiotics that are currently widespread to a variety of communities, such as the campus community, which is equipped with several facilities such as laboratories. The microbiology laboratory is a practicum and research laboratory that uses materials in the form of microorganisms, hence it has the potential to transmit MRSA bacteria. The aim of this study is to find out if MRSA bacteria are present on microbiology lab equipment and how prevalent they are. This study used observational research with a descriptive research design and a purposive sampling strategy. The results of bacterial isolation on MSA medium from fourteen laboratory equipment revealed that thirteen devices exhibited bacterial growth, whereas one device had none. Seven bacterial isolates with S. aureus-like features are detected growing in three of the thirteen equipment, the LAF, incubator, and manual autoclave. The seven bacterial isolates were identified as having round colony shape, glistening, opaque, convex yellow pigmentation, round cell morphology, clustered like grapes and purple in color, positive for coagulase and catalase, and resistance to methicillin antibiotics (MRSA bacteria). It can be concluded that of the fourteen-laboratory equipment examined, three instruments, namely the LAF, incubator, and manual autoclave, were found to be overgrown with MRSA bacteria, with the prevalence of MRSA bacteria in microbiology laboratory equipment reaching 21.4%. Keywords: laboratory equipment, MRSA, prevalence, S. aureus","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"115 17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84192311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-04DOI: 10.30651/jmlt.v4i2.11523
Nastiti Kartikorini, Baterun Kunsah, Diah Ariana
Used cooking oil is the remaining cooking oil from the frying process. The use of cooking oil repeatedly at high temperatures and for a long time causes the peroxide number to increase. High peroxide levels can present a health hazard. Phenolic and flavonoid content contained in sweet orange peel is an antioxidant that acts as an antidote to free radicals so that it can slow down the oxidation of used cooking oil. The purpose of this study was to determine the effectiveness of the long soaking time of the sweet orange peel powder (Citrus sinensis) on the peroxide number in used cooking oil. This type of research is experimental with the number of sample repetitions for each treatment, namely 25 samples. The formulation of the problem of this research is whether there is an effect of the effectiveness of the long soaking time of the sweet orange peel powder (Citrus sinensis) on the peroxide number in used cooking oil. From the results of the examination, the average levels of peroxide numbers in used cooking oil that had been soaked were 1 day 22.7949 mEq, 2 days 19.5965 mEq, 3 days 13.5975 mEq and 4 days 9.5984 mEq. Statistical analysis using ANOVA showed the effect of the effect of soaking duration of sweet orange peel powder (Citrus sinensis) on the peroxide number in used cooking oil p (sig) = 0,000 which is less than 0.05. Keywords: Sweet Orange, Peroxide Number, Used Cooking Oil.
{"title":"Efektivitas Lama Perendaman Serbuk Kulit Jeruk Manis (Citrus sinensis) Terhadap Bilangan Peroksida Pada Minyak Jelantah","authors":"Nastiti Kartikorini, Baterun Kunsah, Diah Ariana","doi":"10.30651/jmlt.v4i2.11523","DOIUrl":"https://doi.org/10.30651/jmlt.v4i2.11523","url":null,"abstract":"Used cooking oil is the remaining cooking oil from the frying process. The use of cooking oil repeatedly at high temperatures and for a long time causes the peroxide number to increase. High peroxide levels can present a health hazard. Phenolic and flavonoid content contained in sweet orange peel is an antioxidant that acts as an antidote to free radicals so that it can slow down the oxidation of used cooking oil. The purpose of this study was to determine the effectiveness of the long soaking time of the sweet orange peel powder (Citrus sinensis) on the peroxide number in used cooking oil. This type of research is experimental with the number of sample repetitions for each treatment, namely 25 samples. The formulation of the problem of this research is whether there is an effect of the effectiveness of the long soaking time of the sweet orange peel powder (Citrus sinensis) on the peroxide number in used cooking oil. From the results of the examination, the average levels of peroxide numbers in used cooking oil that had been soaked were 1 day 22.7949 mEq, 2 days 19.5965 mEq, 3 days 13.5975 mEq and 4 days 9.5984 mEq. Statistical analysis using ANOVA showed the effect of the effect of soaking duration of sweet orange peel powder (Citrus sinensis) on the peroxide number in used cooking oil p (sig) = 0,000 which is less than 0.05. Keywords: Sweet Orange, Peroxide Number, Used Cooking Oil. ","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86761020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-04DOI: 10.30651/jmlt.v4i2.11151
Ria Hanistya, Karima Samlan
Diabetes mellitus is a degenerative disease caused by the damage of beta pancreatic cells. This caused many complication such as diabetic ulcer which left untreated can cause an open wound hard to heal thereby reducing the quality of life. Treatment of diabetic ulcer despite using antidiabetic also using an antibiotic therapy like Mupirocin. In its development, the use of natural ingredients is known to be used as adjuvant therapy for diabetic ulcer such as Ciplukan leaves and Kayu Manis bark. Both of the plant contain phytochemical compound like sinamaldehid, tannin, polyphenol and flavonoid which can work as wound healer. To increase its efficacy then Ciplukan leaves and Kayu Manis bark were formulated as hydrogel patch. Hydrogel patch are known has advantages like better penetration, hygienic and easy to administered Ciplukan (Physalis angulata L.) leaves and Kayu Manis (Cinnamomum burmannii) Bark extracted with maceration technique. The extracts then made into matrix type hydrogel patch with 10% HPMC and 5% PVA as backing patch. The backing patch and extract then placed on a square cast and dried under room temperature for 24 hours. The hydrogel patch then evaluated for physical characteristic including organoleptic aspect, weight uniformity, moisture content and pH. The results showed that Ciplukan leaves and Kayu Manis Bark hydrogel patch have a semi solid consistency, transparent greenish color and distinctive smell. The average pH is 5,5 and have average weight around 1,18 grams. The moisture contens of hydrogel patch is 2,42%. The Ciplukan leaves and Kayu Manis bark is able to formulated into hydrogel patch. The hydrogel patch is showing good appearance and having good properties as topical dosage form. The pH of the hydrogel patch is suitable with pH of the skin and have no intention to irritate the skin. Hydrogel patch also giving a cooling sensations in wound area thus increase patients convenience and acceptability. Keywords: Diabetes mellitus, extract, drug delivery system, hydrogel patch, adjuvant therapy
{"title":"Formulasi Dan Karakteristik Fisik Sediaan Plester Hidrogel Ekstrak Daun Ciplukan (Physalis angulata L.) Dan Batang Kayu Manis (Cinnamomum burmannii)","authors":"Ria Hanistya, Karima Samlan","doi":"10.30651/jmlt.v4i2.11151","DOIUrl":"https://doi.org/10.30651/jmlt.v4i2.11151","url":null,"abstract":"Diabetes mellitus is a degenerative disease caused by the damage of beta pancreatic cells. This caused many complication such as diabetic ulcer which left untreated can cause an open wound hard to heal thereby reducing the quality of life. Treatment of diabetic ulcer despite using antidiabetic also using an antibiotic therapy like Mupirocin. In its development, the use of natural ingredients is known to be used as adjuvant therapy for diabetic ulcer such as Ciplukan leaves and Kayu Manis bark. Both of the plant contain phytochemical compound like sinamaldehid, tannin, polyphenol and flavonoid which can work as wound healer. To increase its efficacy then Ciplukan leaves and Kayu Manis bark were formulated as hydrogel patch. Hydrogel patch are known has advantages like better penetration, hygienic and easy to administered Ciplukan (Physalis angulata L.) leaves and Kayu Manis (Cinnamomum burmannii) Bark extracted with maceration technique. The extracts then made into matrix type hydrogel patch with 10% HPMC and 5% PVA as backing patch. The backing patch and extract then placed on a square cast and dried under room temperature for 24 hours. The hydrogel patch then evaluated for physical characteristic including organoleptic aspect, weight uniformity, moisture content and pH. The results showed that Ciplukan leaves and Kayu Manis Bark hydrogel patch have a semi solid consistency, transparent greenish color and distinctive smell. The average pH is 5,5 and have average weight around 1,18 grams. The moisture contens of hydrogel patch is 2,42%. The Ciplukan leaves and Kayu Manis bark is able to formulated into hydrogel patch. The hydrogel patch is showing good appearance and having good properties as topical dosage form. The pH of the hydrogel patch is suitable with pH of the skin and have no intention to irritate the skin. Hydrogel patch also giving a cooling sensations in wound area thus increase patients convenience and acceptability. Keywords: Diabetes mellitus, extract, drug delivery system, hydrogel patch, adjuvant therapy","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79078161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-04DOI: 10.30651/jmlt.v4i2.11282
Fitrotin Azizah, Dita Artanti
Candidiasis is one of the most common infectious diseases caused by Candida sp. In 2017 the Mycology Division of Dr. Soetomo said there were 67 patients diagnosed with candidiasis. The use of natural ingredients as an alternative to traditional medicine is increasing because it is safe for consumption and does not cause side effects. One of the natural ingredients is Ginger (Zingiber officinale). The choice of using ginger juice (Zingiber officinale) is because the ingredients are easy to obtain and easy to apply by the community. This study aims to determine the antifungal effectiveness of ginger (Zingiber officinale) juice against the growth of Candida albicans. This research is experimental by doing the treatment which was repeated 4 times, namely the concentration of ginger juice 20%, 40%, 60%, 80%, 100%, negative control (aquadest) and positive control (ketoconazole). The inhibition zone examination method used the Kirby Bauer diffusion method which was characterized by the formation of a clear zone around the paper disc on MHA (Muller Hinton Agar) media. The inhibition zone formed was measured using a caliper, then the data obtained were analyzed using the Mann-Whitney test. From laboratory examination, it was found that the average diameter of inhibition formed with concentrations of 20%, 40%, 60%, 80%, and 100% was 0 mm, and the negative control (aquadest) was 0 mm. The results of statistical analysis showed that 0.05 obtained a significant value between the administration of juice concentrations of 20%, 40%, 60%, 80%, 100% and not given (negative control-aquadest) = 1,000. From these results, it was concluded that there was no significant difference between the concentrated juice of 20%, 40%, 60%, 80%, 100% and not given (negative control - distilled water)Keywords : Ginger (Zingiber officinale), Candida albicans
{"title":"Uji Efektifitas Perasan Jahe (Zingiber officinale) Terhadap Pertumbuhan Jamur Candida albicans","authors":"Fitrotin Azizah, Dita Artanti","doi":"10.30651/jmlt.v4i2.11282","DOIUrl":"https://doi.org/10.30651/jmlt.v4i2.11282","url":null,"abstract":"Candidiasis is one of the most common infectious diseases caused by Candida sp. In 2017 the Mycology Division of Dr. Soetomo said there were 67 patients diagnosed with candidiasis. The use of natural ingredients as an alternative to traditional medicine is increasing because it is safe for consumption and does not cause side effects. One of the natural ingredients is Ginger (Zingiber officinale). The choice of using ginger juice (Zingiber officinale) is because the ingredients are easy to obtain and easy to apply by the community. This study aims to determine the antifungal effectiveness of ginger (Zingiber officinale) juice against the growth of Candida albicans. This research is experimental by doing the treatment which was repeated 4 times, namely the concentration of ginger juice 20%, 40%, 60%, 80%, 100%, negative control (aquadest) and positive control (ketoconazole). The inhibition zone examination method used the Kirby Bauer diffusion method which was characterized by the formation of a clear zone around the paper disc on MHA (Muller Hinton Agar) media. The inhibition zone formed was measured using a caliper, then the data obtained were analyzed using the Mann-Whitney test. From laboratory examination, it was found that the average diameter of inhibition formed with concentrations of 20%, 40%, 60%, 80%, and 100% was 0 mm, and the negative control (aquadest) was 0 mm. The results of statistical analysis showed that 0.05 obtained a significant value between the administration of juice concentrations of 20%, 40%, 60%, 80%, 100% and not given (negative control-aquadest) = 1,000. From these results, it was concluded that there was no significant difference between the concentrated juice of 20%, 40%, 60%, 80%, 100% and not given (negative control - distilled water)Keywords : Ginger (Zingiber officinale), Candida albicans","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"213 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73114072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
DNA extraction from dried blood spots was used for molecular analysis. Dried blood spots sample usually used FTA cards, but short-term use would come at a cost. This study aims to identify DNA extraction quality from dried blood spots in Whatman filter paper as an alternative storage sample. This study used 15 samples with three different storage time for 1, 3, and 7 days on 4°C. Dried blood samples were extracted using the Chelex-100 method, and qualitative were identified by electrophoresis. The DNA extraction was used as a template on PCR amplification of the gapdh gene. The result showed that DNA extraction showed band from 1, 3, and 7 days, and PCR amplification showed bands in 200 bp. In conclusions, DNA from dried blood samples was stored for 1, 3 and 7 days in Whatman filter paper were successfully extracted by the Chelex-100 method and can be used as a DNA template for PCR amplification.
{"title":"Uji Kualitas DNA Darah Pada Kertas Whatman Yang Diisolasi Dengan CHELEX-100 Serta Variasi Waktu Penyimpanan","authors":"Rita Maliza, Lutfiana Pratiwi, D. A. Perwitasari","doi":"10.30651/jmlt.v4i2.7936","DOIUrl":"https://doi.org/10.30651/jmlt.v4i2.7936","url":null,"abstract":"DNA extraction from dried blood spots was used for molecular analysis. Dried blood spots sample usually used FTA cards, but short-term use would come at a cost. This study aims to identify DNA extraction quality from dried blood spots in Whatman filter paper as an alternative storage sample. This study used 15 samples with three different storage time for 1, 3, and 7 days on 4°C. Dried blood samples were extracted using the Chelex-100 method, and qualitative were identified by electrophoresis. The DNA extraction was used as a template on PCR amplification of the gapdh gene. The result showed that DNA extraction showed band from 1, 3, and 7 days, and PCR amplification showed bands in 200 bp. In conclusions, DNA from dried blood samples was stored for 1, 3 and 7 days in Whatman filter paper were successfully extracted by the Chelex-100 method and can be used as a DNA template for PCR amplification.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89783948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Infectious diseases are estimated to be the cause of half of all deaths worldwide. About 50-75% of deaths in hospital are reported to be due to infection. This number continues to increase rapidly in line with the development of bacterial resistance to certain antibiotics. One of the causes of resistance due to antibiotic abuse. The number of resistance events that occur encourages new discoveries in order to overcome the problem of resistance. Actinomycetes are Gram-positive microscopic bacteria that are widespread in nature. These bacteria can live in various environmental conditions that contain lots of nutrients. Actinomycetes population increases in the presence of organic matter that undergoes decomposition. Acinomycetes have the ability to produce antimicrobial compounds. The need for antibiotic compounds and chemotherapy compounds that are effective with low toxicity to the host, and the resulting waste can be degraded by the environment, is a problem that needs serious attention. The existence of resistance to antibiotics causes the need to develop new types of antibiotics from natural ingredients and microorganisms that can control microbapatogens. Compounds obtained from natural materials, especially plants and microbes, provide promising results in the development of new antibiotic compounds, among the existing types of microorganisms, Actinomycetes are the most potential source of producing antibiotics. Apart from antibiotics, actinomycetes also produce bioactive compounds of high economic value in the health sector as antiviral and anti-cancer, while in agriculture as herbicides, insecticides, and anti-parasitic compounds. This causes the need to explore actinomycetes which have the potential to produce antibiotic compounds against bacteria. This study aims to determine the antibacterial activity of secondary metabolites of actinomycetes isolates against cuts infected with Staphylococcus aureus resistant methicillin (MRSA) in winstar rats (rattus norvegicus). This type of research is carried out in an experimental laboratory. The results showed that the potential for secondary metabolites of actinomysetes isolates against MRSA bacteria was classified as strong with an inhibitory index of 10.1.
{"title":"Potensi Metabolit Sekunder Isolat Aktinomycetes Sebagai Penghasil Senyawa Antibakteri Terhadap Methicillin Resistant Staphylococcus aureus (MRSA) Dari Tanah Mangrove Wonorejo Surabaya","authors":"Anita Dwi Anggraini, Ayu Puspitasari, Christ Kartika Rahayuningsih","doi":"10.30651/jmlt.v4i2.7672","DOIUrl":"https://doi.org/10.30651/jmlt.v4i2.7672","url":null,"abstract":"Infectious diseases are estimated to be the cause of half of all deaths worldwide. About 50-75% of deaths in hospital are reported to be due to infection. This number continues to increase rapidly in line with the development of bacterial resistance to certain antibiotics. One of the causes of resistance due to antibiotic abuse. The number of resistance events that occur encourages new discoveries in order to overcome the problem of resistance. Actinomycetes are Gram-positive microscopic bacteria that are widespread in nature. These bacteria can live in various environmental conditions that contain lots of nutrients. Actinomycetes population increases in the presence of organic matter that undergoes decomposition. Acinomycetes have the ability to produce antimicrobial compounds. The need for antibiotic compounds and chemotherapy compounds that are effective with low toxicity to the host, and the resulting waste can be degraded by the environment, is a problem that needs serious attention. The existence of resistance to antibiotics causes the need to develop new types of antibiotics from natural ingredients and microorganisms that can control microbapatogens. Compounds obtained from natural materials, especially plants and microbes, provide promising results in the development of new antibiotic compounds, among the existing types of microorganisms, Actinomycetes are the most potential source of producing antibiotics. Apart from antibiotics, actinomycetes also produce bioactive compounds of high economic value in the health sector as antiviral and anti-cancer, while in agriculture as herbicides, insecticides, and anti-parasitic compounds. This causes the need to explore actinomycetes which have the potential to produce antibiotic compounds against bacteria. This study aims to determine the antibacterial activity of secondary metabolites of actinomycetes isolates against cuts infected with Staphylococcus aureus resistant methicillin (MRSA) in winstar rats (rattus norvegicus). This type of research is carried out in an experimental laboratory. The results showed that the potential for secondary metabolites of actinomysetes isolates against MRSA bacteria was classified as strong with an inhibitory index of 10.1.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89920643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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