Pub Date : 2022-12-03DOI: 10.30651/jmlt.v5i2.13478
Prasasti Kusumaning Paramastri, Muhammad Taufiq Qurrohman
Candidiasis is a disease caused by Candida albicans, this disease has a mortality rate of > 25%, mainly affecting adults, with a frequency of women 2 - 3 times more than men (Raningsih et al., 2018). A commonly used fungi drug is ketoconazole. but it has many side effects so it is necessary to find for alternatives to natural ingredients, namely the leaf of lidah mertua (Sansevieria trifasciata var laurentii) because it contains compounds saponins, flavonoids, triterpenoids, and steroids as antifungals The leaf of lidah mertua were extracted by maceration technique. The purpose of this research was to determine the effect of antifungi of ethanol extract the leaf of Lidah Mertua (Sansiviera trifasciata var Laurentii) against the fungus Candida albicans. This research was experimental using the disc diffusion method with a spread plate technique in the inhibition zone formed at concentrations of 25%, 20%, 15%, 10%, 5%, positive control and negative control. The results of the One way Anova test is known that differ significantly. From the results of this research, it can be concluded that the ethanol extract of the leaves of the leaf of lidah mertua (Sansevieria trifasciata var laurentii) has an effect on the antifungal activity of Candida albicans Keywords : Candida albicans, Leaf of Lidah Mertua, Antifungal, Ketokonazole
念珠菌病是由白色念珠菌引起的疾病，该病死亡率> 25%，主要影响成人，女性发病频率是男性的2 - 3倍(Raningsih et al.， 2018)。一种常用的真菌药物是酮康唑。但它有许多副作用，因此有必要寻找天然成分的替代品，即山茱萸叶(Sansevieria trifasciata var laurentii)，因为它含有化合物皂苷、黄酮类化合物、三萜和类固醇等抗真菌物质。本研究的目的是研究三叶莲叶乙醇提取物对白色念珠菌的抑菌作用。本研究在浓度为25%、20%、15%、10%、5%、阳性对照和阴性对照形成的抑菌带中，采用盘片扩散法和扩散板技术进行实验。已知单向方差分析的结果差异显著。从本研究结果可以看出，白念珠菌提取物对白色念珠菌具有一定的抗真菌活性。关键词:白色念珠菌，白念珠菌，抗真菌，酮康唑
{"title":"Efektifitas Ekstrak Lidah Mertua (Sansevieria trifasciata var laurentii) Sebagai Antifungi Candida albicans","authors":"Prasasti Kusumaning Paramastri, Muhammad Taufiq Qurrohman","doi":"10.30651/jmlt.v5i2.13478","DOIUrl":"https://doi.org/10.30651/jmlt.v5i2.13478","url":null,"abstract":"Candidiasis is a disease caused by Candida albicans, this disease has a mortality rate of > 25%, mainly affecting adults, with a frequency of women 2 - 3 times more than men (Raningsih et al., 2018). A commonly used fungi drug is ketoconazole. but it has many side effects so it is necessary to find for alternatives to natural ingredients, namely the leaf of lidah mertua (Sansevieria trifasciata var laurentii) because it contains compounds saponins, flavonoids, triterpenoids, and steroids as antifungals The leaf of lidah mertua were extracted by maceration technique. The purpose of this research was to determine the effect of antifungi of ethanol extract the leaf of Lidah Mertua (Sansiviera trifasciata var Laurentii) against the fungus Candida albicans. This research was experimental using the disc diffusion method with a spread plate technique in the inhibition zone formed at concentrations of 25%, 20%, 15%, 10%, 5%, positive control and negative control. The results of the One way Anova test is known that differ significantly. From the results of this research, it can be concluded that the ethanol extract of the leaves of the leaf of lidah mertua (Sansevieria trifasciata var laurentii) has an effect on the antifungal activity of Candida albicans Keywords : Candida albicans, Leaf of Lidah Mertua, Antifungal, Ketokonazole","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87195881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-03DOI: 10.30651/jmlt.v5i2.14407
Muhammad Sholakhuddin Al-Ayubi, Pestariati Pestariati, Anita Dwi Anggraini, Diah Titik Mutiarawati
Media pertumbuhan yang mengandung nutrisi yang dibutuhkan oleh bakteri dan yang sering digunakan untuk pertumbuhan bakteri yaitu media Nutrient Agar. Mahalnya harga media pertumbuhan bakteri mendorong para peneliti untuk menggunakan media pertumbuhan bakteri yang terbuat dari bahan-bahan alami yang mudah didapatkan dan tidak mengeluarkan biaya yang mahal. Ikan tongkol memiliki kandungan protein sebesar 26,46% dan ikan lele yang mempunyai kandungan protein sebesar 24,63%. Escherichia coli merupakan bakteri Gram negatif bersel satu, bersifat patogen dan dapat menyebabkan penyakit diare. Jenis penelitian yang dilakukan adalah Eksperimen. Hasil penelitian ini menunjukkan jumlah rata-rata koloni terbanyak pada pertumbuhan Escherichia coli dengan menggunakan media alternatif Ikan Tongkol yaitu pada variasi massa 5 gram yaitu 118 x 10-13 CFU/mL, sedangkan jumlah rata-rata koloni terbanyak pada pertumbuhan Escherichia coli dengan menggunakan media alternatif Ikan Lele yaitu pada variasi massa 5 gram yaitu 104 x 10-13 CFU/mL. Media substitusi Nutrient Agar dari tepung daging Ikan Tongkol dan Ikan Lele dengan variasi massa 1 gram, 3 gram dan 5 gram dapat menumbuhkan koloni bakteri Escherichia coli dengan jumlah dan karakteristik yang berbeda. Hal tersebut disebabkan oleh kandungan protein di dalam tepung daging Ikan Tongkol dan Ikan Lele yang dapat memenuhi kebutuhan nutrisi pertumbuhan Escherichia coli. Ikan tongkol dan ikan lele mempunyai potensi sebagai bahan dasar pembuatan media alternatif untuk pertumbuhan bakteri Escherichia coli ditandai dengan adanya pertumbuhan koloni bakteri pada media alternatif ikan tongkol dan ikan lele. Kata Kunci : Ikan Tongkol, Ikan Lele, Nutrient Agar, Escherichia coli
{"title":"Potensi Ikan Tongkol Dan Ikan Lele Sebagai Media Alternatif Pertumbuhan Escherichia coli","authors":"Muhammad Sholakhuddin Al-Ayubi, Pestariati Pestariati, Anita Dwi Anggraini, Diah Titik Mutiarawati","doi":"10.30651/jmlt.v5i2.14407","DOIUrl":"https://doi.org/10.30651/jmlt.v5i2.14407","url":null,"abstract":"Media pertumbuhan yang mengandung nutrisi yang dibutuhkan oleh bakteri dan yang sering digunakan untuk pertumbuhan bakteri yaitu media Nutrient Agar. Mahalnya harga media pertumbuhan bakteri mendorong para peneliti untuk menggunakan media pertumbuhan bakteri yang terbuat dari bahan-bahan alami yang mudah didapatkan dan tidak mengeluarkan biaya yang mahal. Ikan tongkol memiliki kandungan protein sebesar 26,46% dan ikan lele yang mempunyai kandungan protein sebesar 24,63%. Escherichia coli merupakan bakteri Gram negatif bersel satu, bersifat patogen dan dapat menyebabkan penyakit diare. Jenis penelitian yang dilakukan adalah Eksperimen. Hasil penelitian ini menunjukkan jumlah rata-rata koloni terbanyak pada pertumbuhan Escherichia coli dengan menggunakan media alternatif Ikan Tongkol yaitu pada variasi massa 5 gram yaitu 118 x 10-13 CFU/mL, sedangkan jumlah rata-rata koloni terbanyak pada pertumbuhan Escherichia coli dengan menggunakan media alternatif Ikan Lele yaitu pada variasi massa 5 gram yaitu 104 x 10-13 CFU/mL. Media substitusi Nutrient Agar dari tepung daging Ikan Tongkol dan Ikan Lele dengan variasi massa 1 gram, 3 gram dan 5 gram dapat menumbuhkan koloni bakteri Escherichia coli dengan jumlah dan karakteristik yang berbeda. Hal tersebut disebabkan oleh kandungan protein di dalam tepung daging Ikan Tongkol dan Ikan Lele yang dapat memenuhi kebutuhan nutrisi pertumbuhan Escherichia coli. Ikan tongkol dan ikan lele mempunyai potensi sebagai bahan dasar pembuatan media alternatif untuk pertumbuhan bakteri Escherichia coli ditandai dengan adanya pertumbuhan koloni bakteri pada media alternatif ikan tongkol dan ikan lele. Kata Kunci : Ikan Tongkol, Ikan Lele, Nutrient Agar, Escherichia coli","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"841 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90425698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-29DOI: 10.30651/jmlt.v5i1.11280
S. Kesuma, Suparno Putera Makkadafi
SARS CoV-2 infection, which has affected the world since late 2019, can cause serious lower respiratory tract infections that may be fatal in some patients. This infection causes the disease Covid-19. The diagnosis of SARS-CoV-2 infection was carried out by NAAT (Nucleic Acid Amplification Test) such as RT-PCR examination. The sample needed for the identification of SARS-COV-2 is a nasopharyngeal/oropharyngeal swab. Nasopharyngeal/oropharyngeal swab sampling requires trained personnel. Taking a nasopharyngeal/oropharyngeal swab is invasive, causing discomfort in its implementation. The convenience of sampling specimens can be an alternative option for the identification of SARS-CoV-2, such as with newly developed biosaliva specimens. The use of this biosaliva sample can be a practical option in the examination of the identification of SARS-CoV-2. However, the use of these specimens needs to be evaluated first because of the possible relationship with clinical findings and so that the results of the SARS-CoV-2 examination are valid and reliable. The purpose of this study was to evaluate the use of biosaliva specimens to detect SARS-CoV-2 infection with the RT-PCR method. Evaluation of the use of biosaliva in the detection of SARS-COV-2 RT-PCR method with paired T test and diagnostic test with the gold standard using nasopharyngeal/oropharyngeal swabs. The target genes for the detection of SARS-CoV-2 are the RdRp gene and the E gene with control of the HRP gene. RT-PCR was carried out with 40 cycles and Tm 62 °C. The results of this study are Sig. (2-tailed) paired T test was 0.106, sensitivity was 64.86% and specificity was 90.92%. The conclusion of this study is that there is no statistical difference in the results of the SARS-CoV-2 RT-PCR method between the use of biosaliva specimens and nasopharyngeal/oropharyngeal swabs, and the evaluation results show that reliable biosaliva specimens are used as samples in the examination of SARS-COV-2 infection.
{"title":"Evaluasi Penggunaan Biosaliva Dalam Deteksi Sars-Cov-2 Metode RT-PCR","authors":"S. Kesuma, Suparno Putera Makkadafi","doi":"10.30651/jmlt.v5i1.11280","DOIUrl":"https://doi.org/10.30651/jmlt.v5i1.11280","url":null,"abstract":"SARS CoV-2 infection, which has affected the world since late 2019, can cause serious lower respiratory tract infections that may be fatal in some patients. This infection causes the disease Covid-19. The diagnosis of SARS-CoV-2 infection was carried out by NAAT (Nucleic Acid Amplification Test) such as RT-PCR examination. The sample needed for the identification of SARS-COV-2 is a nasopharyngeal/oropharyngeal swab. Nasopharyngeal/oropharyngeal swab sampling requires trained personnel. Taking a nasopharyngeal/oropharyngeal swab is invasive, causing discomfort in its implementation. The convenience of sampling specimens can be an alternative option for the identification of SARS-CoV-2, such as with newly developed biosaliva specimens. The use of this biosaliva sample can be a practical option in the examination of the identification of SARS-CoV-2. However, the use of these specimens needs to be evaluated first because of the possible relationship with clinical findings and so that the results of the SARS-CoV-2 examination are valid and reliable. The purpose of this study was to evaluate the use of biosaliva specimens to detect SARS-CoV-2 infection with the RT-PCR method. Evaluation of the use of biosaliva in the detection of SARS-COV-2 RT-PCR method with paired T test and diagnostic test with the gold standard using nasopharyngeal/oropharyngeal swabs. The target genes for the detection of SARS-CoV-2 are the RdRp gene and the E gene with control of the HRP gene. RT-PCR was carried out with 40 cycles and Tm 62 °C. The results of this study are Sig. (2-tailed) paired T test was 0.106, sensitivity was 64.86% and specificity was 90.92%. The conclusion of this study is that there is no statistical difference in the results of the SARS-CoV-2 RT-PCR method between the use of biosaliva specimens and nasopharyngeal/oropharyngeal swabs, and the evaluation results show that reliable biosaliva specimens are used as samples in the examination of SARS-COV-2 infection.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80121688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-29DOI: 10.30651/jmlt.v5i1.11015
I. W. B. Suyasa, Herijanto Bekti, Luh Putu Rinawati, Luh Putu Laksmi, Putu Diah Wahyuni, Desak Gede Dwi Agustini, A. Rakhmawati
Staphylococcus aureus is one of the causes of infectious disease, ranging from mild to severe infections such as meningitis. The drug that is often used is the penicillin class of antibiotics. Apart from chemical antibiotics, natural antibacterial compunds from plants can be also used as an alternative treatment for this infection. Such as green betel (Piper betle L.) and legundi (Vitex trifolia L.). The ethanol extract of betel leaf has antibacterial activity against Staphylococcus aureus. The phytochemical content in legundi leaf is also able to inhibit the growth of Staphylococcus aureus. The ethanol extract of betel leaf and legundi is made through a maceration process. The extract concentrations used in this study were 20%, 30%, and 40%. The inhibition test of Staphylococcus aureus using the disc method. From the results of this study, the active substances found in betel leaf were flavonoids, tannins, and phenols, while in legundi were found flavonoids, tannins and phenols, and quinones. In total, the mean inhibition zone in this study ranged from 7.87mm to 17.33mm where legundi leaf extract was in the moderate category, betel and combination extract were classified as strong. There was a difference in the diameter of the inhibition zone of betel and legundi extract at a concentration 20% with 40%. In the combination extract there is a difference in the concentration of 20% with 40% and 30% with 40%. An antimicrobial compound can be said to synergize if a mixture of two or more antimicrobial compounds is able to provide an effect of each compounds. In this study, it has seen that the mean of the combination is higher than the mean of legundi leaf extract. Meanwhile, the mean of betel leaf extract is still greater than the combination extract.
{"title":"Daya Hambat Ekstrak Daun Sirih dan Daun Legundi Terhadap Pertumbuhan Bakteri Staphylococcus aureus","authors":"I. W. B. Suyasa, Herijanto Bekti, Luh Putu Rinawati, Luh Putu Laksmi, Putu Diah Wahyuni, Desak Gede Dwi Agustini, A. Rakhmawati","doi":"10.30651/jmlt.v5i1.11015","DOIUrl":"https://doi.org/10.30651/jmlt.v5i1.11015","url":null,"abstract":"Staphylococcus aureus is one of the causes of infectious disease, ranging from mild to severe infections such as meningitis. The drug that is often used is the penicillin class of antibiotics. Apart from chemical antibiotics, natural antibacterial compunds from plants can be also used as an alternative treatment for this infection. Such as green betel (Piper betle L.) and legundi (Vitex trifolia L.). The ethanol extract of betel leaf has antibacterial activity against Staphylococcus aureus. The phytochemical content in legundi leaf is also able to inhibit the growth of Staphylococcus aureus. The ethanol extract of betel leaf and legundi is made through a maceration process. The extract concentrations used in this study were 20%, 30%, and 40%. The inhibition test of Staphylococcus aureus using the disc method. From the results of this study, the active substances found in betel leaf were flavonoids, tannins, and phenols, while in legundi were found flavonoids, tannins and phenols, and quinones. In total, the mean inhibition zone in this study ranged from 7.87mm to 17.33mm where legundi leaf extract was in the moderate category, betel and combination extract were classified as strong. There was a difference in the diameter of the inhibition zone of betel and legundi extract at a concentration 20% with 40%. In the combination extract there is a difference in the concentration of 20% with 40% and 30% with 40%. An antimicrobial compound can be said to synergize if a mixture of two or more antimicrobial compounds is able to provide an effect of each compounds. In this study, it has seen that the mean of the combination is higher than the mean of legundi leaf extract. Meanwhile, the mean of betel leaf extract is still greater than the combination extract.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"8 353 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76127117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT A high prevalence of TB requires serious treatment with Anti-Tuberculosis Therapy, but the side effects can gain uric acid levels. This study aimed to analyze the effects of intensive phase therapy and high purine consumption patterns with uric acid levels in TB patients with Anti-Tuberculosis Therapy. This research was an analytic study using a prospective cohort design. The population was all tuberculosis patients who received Anti-Tuberculosis Therapy at several Public Health Centers in Samarinda. The sampling technique used consecutive sampling approach. The respondents were selected according to the inclusion criteria and had signed the informed consent. Furthermore, the venous blood was measured to know the levels of the uric acid pre-test. The measurements of uric acid levels post-test were carried out after undergoing Anti-Tuberculosis Therapy for 2 months and filling out the FFQ questionnaire to determine the patterns of high purine consumption. The collected data was analyzed using a t-paired and a one-way anova test. T-Paired test results exhibited that there were increasing trend in the average uric acid levels after following Anti-Tuberculosis Therapy in an intensive phase of 2.53 mg / dL (39.78%) with p-value <0.001. Besides, the one way anova test results showed that the average difference in uric acid levels in respondents who consumed high purine 4-6 times/week was 3.16 mg / dL and was higher than respondents who never consumed high purine or those who consumed high purine 1-3 times/week with a p-value <0.001. An average increase in uric acid levels in TB patients after undergoing the intensive phase of Anti-Tuberculosis Therapy for 2 months with a pyrazinamide and ethambutol regimen and an increase in uric acid levels was higher compared to respondents who consumed high purine. Keywords: uric acid level, high purine consumption, intensive phase
{"title":"Efek Obat Antituberkulosis Fase Intensif dan Konsumsi Purin Terhadap Kadar Asam Urat Pasien Tuberkulosis","authors":"Mustaming Mustaming","doi":"10.30651/jmlt.v5i1.7939","DOIUrl":"https://doi.org/10.30651/jmlt.v5i1.7939","url":null,"abstract":"ABSTRACT A high prevalence of TB requires serious treatment with Anti-Tuberculosis Therapy, but the side effects can gain uric acid levels. This study aimed to analyze the effects of intensive phase therapy and high purine consumption patterns with uric acid levels in TB patients with Anti-Tuberculosis Therapy. This research was an analytic study using a prospective cohort design. The population was all tuberculosis patients who received Anti-Tuberculosis Therapy at several Public Health Centers in Samarinda. The sampling technique used consecutive sampling approach. The respondents were selected according to the inclusion criteria and had signed the informed consent. Furthermore, the venous blood was measured to know the levels of the uric acid pre-test. The measurements of uric acid levels post-test were carried out after undergoing Anti-Tuberculosis Therapy for 2 months and filling out the FFQ questionnaire to determine the patterns of high purine consumption. The collected data was analyzed using a t-paired and a one-way anova test. T-Paired test results exhibited that there were increasing trend in the average uric acid levels after following Anti-Tuberculosis Therapy in an intensive phase of 2.53 mg / dL (39.78%) with p-value <0.001. Besides, the one way anova test results showed that the average difference in uric acid levels in respondents who consumed high purine 4-6 times/week was 3.16 mg / dL and was higher than respondents who never consumed high purine or those who consumed high purine 1-3 times/week with a p-value <0.001. An average increase in uric acid levels in TB patients after undergoing the intensive phase of Anti-Tuberculosis Therapy for 2 months with a pyrazinamide and ethambutol regimen and an increase in uric acid levels was higher compared to respondents who consumed high purine. Keywords: uric acid level, high purine consumption, intensive phase","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82059516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Healthy and nutritious food should be free from biological contaminants. The presence of Escherichia coli in food is an indicator contamination and also presence of other pathogenic bacteria. This study aims to determine the value of MPN E. coli in food samples using the MPN-PCR combination technique. Descriptive research method. Samples were taken from 5 traders in Malang City. The results showed that 3 out of 5 food samples were contaminated with E. coli, indicated by the presence of a specific band amplified and produced a DNA band measuring 584 base pairs in agarose gel electrophoresis. Conclusion: MPN value of E. coli in the three food samples code II 34 MPN / gram, code III 75 MPN / gram and V code 290 MPN / gram. The MPN-PCR combination can be used as an alternative method for the conventional MPN test because it can detect the presence of E. coli bacteria in food quickly and reliably
{"title":"Identifikasi dan Enumerasi Escherichia coli dengan Kombinasi Metode MPN-PCR","authors":"Atik Kurniawati, Dwi Listyorini, Agung Witjoro","doi":"10.30651/jmlt.v5i1.7592","DOIUrl":"https://doi.org/10.30651/jmlt.v5i1.7592","url":null,"abstract":"Healthy and nutritious food should be free from biological contaminants. The presence of Escherichia coli in food is an indicator contamination and also presence of other pathogenic bacteria. This study aims to determine the value of MPN E. coli in food samples using the MPN-PCR combination technique. Descriptive research method. Samples were taken from 5 traders in Malang City. The results showed that 3 out of 5 food samples were contaminated with E. coli, indicated by the presence of a specific band amplified and produced a DNA band measuring 584 base pairs in agarose gel electrophoresis. Conclusion: MPN value of E. coli in the three food samples code II 34 MPN / gram, code III 75 MPN / gram and V code 290 MPN / gram. The MPN-PCR combination can be used as an alternative method for the conventional MPN test because it can detect the presence of E. coli bacteria in food quickly and reliably","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78589899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-29DOI: 10.30651/jmlt.v5i1.10393
Chintia Agatha Oktavia Pah
Batang bambu (Bambusa sp) biasa digunakan dibidang industri,tetapi batang bambu juga dapat diolah sebagai asap cair karena memiliki senyawa alami yaitu fenol, asam asetat dan karbonil yang dapat digunakan sebagai antimikroba. Penelitian ini bertujuan untuk mengurangi bahan kimia dalam penggunaan antiseptik. Dilakukan uji efeltivitas asap cair batang bambu secara in vitro dan in vivo, secara in vitro dilakukan dengan metode kirby bauer, metode ini terdiri dari difusi cakram untuk bakteri dengan jamur, dan metode sumuran untuk jamur, dengan alkohol 70% sebagai kontrol positif dan akuades sebagai kontrol negatif, konsentrasi asap cair yang digunakan adalah 50%, 75%, dan 100% untuk mengetahui konsentrasi optimum dalam menghambat pertumbuhan S.aureus ATCC 25923, E.coli ATCC 25922, C.albicans ATCC 10231 dan A.flavus ATCC 9643. Sementara itu, uji efektivitas secara in vivo dilakukan dengan mengaplikasikan asap cair batang bambu konsentrasi 100% pada telapak tangan responden. Perhitungan jumlah koloni bakteri dan jamur dilakukan sebelum dan setelah diberi perlakuan. Metode yang digunakan adalah Total Plate Count (TPC). Hasil asap cair batang bambu secara in vitro menunjukkan adanya zona hambat pada konsentrasi optimum 100% dengan nilai rata-rata S.aureus ATCC 25923 (6,83 mm), E.coli ATCC 25922 (3,3 mm) sedangkan pada C.albicans ATCC10231 dan A.flavus ATCC 9643 tidak terdapat zona hambat pada konsentrasi optimum 100%. Hasil secara in vivo menunjukkan asap cair batang bambu konsentrasi 100% efektif dalam menghambat pertumbuhan bakteri sebesar 82,3% dan jamur sebesar 73%. Hasil penelitian in vivo didukung dengan hasil kuesioner kesukaan responden terhadap asap cair batang bambu yaitu diantaranya suka terhadap warna 91,6%, terhadap aroma 75%, suka terhadap asap cair karena tidak menimbulkan efek kekeringan pada tangan sebesar 91,7% dan tidak menimbulkan efek lainnya (seperti sensasi terbakar) 100%.
{"title":"Uji Efektivitas Asap Cair Batang Bambu (Bambusa sp) sebagai Antiseptik","authors":"Chintia Agatha Oktavia Pah","doi":"10.30651/jmlt.v5i1.10393","DOIUrl":"https://doi.org/10.30651/jmlt.v5i1.10393","url":null,"abstract":"Batang bambu (Bambusa sp) biasa digunakan dibidang industri,tetapi batang bambu juga dapat diolah sebagai asap cair karena memiliki senyawa alami yaitu fenol, asam asetat dan karbonil yang dapat digunakan sebagai antimikroba. Penelitian ini bertujuan untuk mengurangi bahan kimia dalam penggunaan antiseptik. Dilakukan uji efeltivitas asap cair batang bambu secara in vitro dan in vivo, secara in vitro dilakukan dengan metode kirby bauer, metode ini terdiri dari difusi cakram untuk bakteri dengan jamur, dan metode sumuran untuk jamur, dengan alkohol 70% sebagai kontrol positif dan akuades sebagai kontrol negatif, konsentrasi asap cair yang digunakan adalah 50%, 75%, dan 100% untuk mengetahui konsentrasi optimum dalam menghambat pertumbuhan S.aureus ATCC 25923, E.coli ATCC 25922, C.albicans ATCC 10231 dan A.flavus ATCC 9643. Sementara itu, uji efektivitas secara in vivo dilakukan dengan mengaplikasikan asap cair batang bambu konsentrasi 100% pada telapak tangan responden. Perhitungan jumlah koloni bakteri dan jamur dilakukan sebelum dan setelah diberi perlakuan. Metode yang digunakan adalah Total Plate Count (TPC). Hasil asap cair batang bambu secara in vitro menunjukkan adanya zona hambat pada konsentrasi optimum 100% dengan nilai rata-rata S.aureus ATCC 25923 (6,83 mm), E.coli ATCC 25922 (3,3 mm) sedangkan pada C.albicans ATCC10231 dan A.flavus ATCC 9643 tidak terdapat zona hambat pada konsentrasi optimum 100%. Hasil secara in vivo menunjukkan asap cair batang bambu konsentrasi 100% efektif dalam menghambat pertumbuhan bakteri sebesar 82,3% dan jamur sebesar 73%. Hasil penelitian in vivo didukung dengan hasil kuesioner kesukaan responden terhadap asap cair batang bambu yaitu diantaranya suka terhadap warna 91,6%, terhadap aroma 75%, suka terhadap asap cair karena tidak menimbulkan efek kekeringan pada tangan sebesar 91,7% dan tidak menimbulkan efek lainnya (seperti sensasi terbakar) 100%.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80072344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-29DOI: 10.30651/jmlt.v5i1.12667
Puspitasari Puspitasari, Andika Aliviameita
Sample stability, part of the pre-analytic phase is an important component that can affect clinical laboratory results. Handling of blood samples and storage methods can significantly affect the results of hematological examinations. This study aims to determine the effect of delay time and storage temperature on the results of the examination of leukocyte count, hemoglobin level, erythrocyte count, hematocrit level, and platelet count. The type of research used is a quantitative analysis using laboratory experimental methods. The research was carried out in the hematology laboratory of the Medical Laboratory Technology Study Program, University Muhammadiyah of Sidoarjo in September - December 2021. The results of the Two Way ANOVA test showed that there was no statistically significant effect between the length of time delay and storage temperature on the results of the examination of leukocyte count, hemoglobin level, number of erythrocytes. Friedman test results showed that there was a statistically significant effect between the length of time of delay and storage temperature on hematocrit levels and platelet counts.
{"title":"Stabilitas Sampel Darah Terhadap Profil Hematologi Dengan Metode Otomatis","authors":"Puspitasari Puspitasari, Andika Aliviameita","doi":"10.30651/jmlt.v5i1.12667","DOIUrl":"https://doi.org/10.30651/jmlt.v5i1.12667","url":null,"abstract":"Sample stability, part of the pre-analytic phase is an important component that can affect clinical laboratory results. Handling of blood samples and storage methods can significantly affect the results of hematological examinations. This study aims to determine the effect of delay time and storage temperature on the results of the examination of leukocyte count, hemoglobin level, erythrocyte count, hematocrit level, and platelet count. The type of research used is a quantitative analysis using laboratory experimental methods. The research was carried out in the hematology laboratory of the Medical Laboratory Technology Study Program, University Muhammadiyah of Sidoarjo in September - December 2021. The results of the Two Way ANOVA test showed that there was no statistically significant effect between the length of time delay and storage temperature on the results of the examination of leukocyte count, hemoglobin level, number of erythrocytes. Friedman test results showed that there was a statistically significant effect between the length of time of delay and storage temperature on hematocrit levels and platelet counts.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75457623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diabetes mellitus is a chronic metabolic disease characterized by a state of hyperglycemia due to a lack of insulin production by the pancreas or the body's inability to use the insulin produced or both. Uncontrolled hyperglycemia can suppress the immune system and making the body vulnerable to infection, especially urinary tract infections (UTIs). The main choice in UTI treatment is antibiotic therapy, one of the most frequently used is ciprofloxacin from the fluoroquinolone group.The research design used was descriptive method with prevalence studies. The research was done using antibiotic sensitivity tests with agar diffusion methods. The number of samples were taken using total sampling method.The results of ciprofloxacin sensitivity test were obtained as many as 22 isolates. Escherichia coli bacteria are sensitive to ciprofloxacin at 66.67%, intermediate and resistant 16.67% respectively. Pseudomonas aeruginosa are 60% sensitive and 40% intermediate. Enterobacter aerogenes are 66,67% sensitive and 33,33% intermediate. Shigella spp. are 100% sensitive, and Klebsiella spp. 100% resistant.The conclusion from the study showed that ciprofloxacin are an effective empirical therapy for UTI.
{"title":"Description Of Ciprofloxacin Sensitivity On Bacteria Causing UTI In T2DM Patients","authors":"Muhammad Ibnu Nazari","doi":"10.30651/jmlt.v5i1.6628","DOIUrl":"https://doi.org/10.30651/jmlt.v5i1.6628","url":null,"abstract":"Diabetes mellitus is a chronic metabolic disease characterized by a state of hyperglycemia due to a lack of insulin production by the pancreas or the body's inability to use the insulin produced or both. Uncontrolled hyperglycemia can suppress the immune system and making the body vulnerable to infection, especially urinary tract infections (UTIs). The main choice in UTI treatment is antibiotic therapy, one of the most frequently used is ciprofloxacin from the fluoroquinolone group.The research design used was descriptive method with prevalence studies. The research was done using antibiotic sensitivity tests with agar diffusion methods. The number of samples were taken using total sampling method.The results of ciprofloxacin sensitivity test were obtained as many as 22 isolates. Escherichia coli bacteria are sensitive to ciprofloxacin at 66.67%, intermediate and resistant 16.67% respectively. Pseudomonas aeruginosa are 60% sensitive and 40% intermediate. Enterobacter aerogenes are 66,67% sensitive and 33,33% intermediate. Shigella spp. are 100% sensitive, and Klebsiella spp. 100% resistant.The conclusion from the study showed that ciprofloxacin are an effective empirical therapy for UTI.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81299723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-29DOI: 10.30651/jmlt.v5i1.10870
A. A. Biomi, R. Yuan
As technology develops, air quality tends to decline. One of the air pollutants that is often encountered is bacteria that infect through the air such as Staphylococcus aureus. As a basic protection tool, a cloth mask with a membrane layer can be an alternative solution. One of the materials that can be used is chitosan and lime peel pectin. Lime peel produces essential oils that are used as stimulant, aromatherapy, and antimicrobial enhancers. This study aims to determine the results of characteristic tests, content analysis through phytochemicals and Staphylococcus aureus. The research method used is descriptive exploratory and experimental. The membrane mask made from chitosan of shrimp skin and lime peel has a characteristic thickness of 00.24 mm with a density of 0.60 - 0.90 g/cm3 with a textured heterogeneous surface. Has a water vapor transmission power of 0.00088 g/day/m2. It takes 27.54 days to completely decompose. Grapefruit peel essential oil contains secondary metabolites in the form of alkaloids, flavonoids, tannins, phenolics and triterpenoids. The membrane mask had Staphylococcus aureus antibacterial activity ranging from 14.05 mm. Keywords: Lime peel (Citrus aurantiifolia), Shrimp Skin Chitosan, Membrane mask, Antibacterial.
{"title":"Pembuatan Membran Antiseptik Dari Kombinasi Kitosan Dan Kulit Jeruk Nipis (Citrus aurantiifolia","authors":"A. A. Biomi, R. Yuan","doi":"10.30651/jmlt.v5i1.10870","DOIUrl":"https://doi.org/10.30651/jmlt.v5i1.10870","url":null,"abstract":"As technology develops, air quality tends to decline. One of the air pollutants that is often encountered is bacteria that infect through the air such as Staphylococcus aureus. As a basic protection tool, a cloth mask with a membrane layer can be an alternative solution. One of the materials that can be used is chitosan and lime peel pectin. Lime peel produces essential oils that are used as stimulant, aromatherapy, and antimicrobial enhancers. This study aims to determine the results of characteristic tests, content analysis through phytochemicals and Staphylococcus aureus. The research method used is descriptive exploratory and experimental. The membrane mask made from chitosan of shrimp skin and lime peel has a characteristic thickness of 00.24 mm with a density of 0.60 - 0.90 g/cm3 with a textured heterogeneous surface. Has a water vapor transmission power of 0.00088 g/day/m2. It takes 27.54 days to completely decompose. Grapefruit peel essential oil contains secondary metabolites in the form of alkaloids, flavonoids, tannins, phenolics and triterpenoids. The membrane mask had Staphylococcus aureus antibacterial activity ranging from 14.05 mm. Keywords: Lime peel (Citrus aurantiifolia), Shrimp Skin Chitosan, Membrane mask, Antibacterial.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89409089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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