Pub Date : 2023-05-19DOI: 10.30651/jmlt.v6i1.12441
Lisa Yunita, Dian Rachma Wijayanti, Apriani Riyanti
Covid-19 (Corona Virus disease 2019) is a new type of disease caused by a virus from the Corona Virus group, namely SARS-CoV-2. Covid-19 may cause respiratory system disorders, ranging from mild symptoms such as flu to lung infections, such as pneumonia. Laboratory diagnoses for Covid-19 disease generally include hematological (complete blood examinations), and molecular or a combination of serology and molecular. PCR examination that can be carried out using the Insulated Isothermal PCR and Reverse Transcription PCR methods. This research is descriptive observational research. Data were collected at Grha Kedoya Hospital, West Jakarta, North Kedoya. The result showed a sensitivity of iiPCR score 82,6%, which is relatively lower than gold-standard RT-PCR. Our research suggested that RT-PCR is still an effective and sensitive method for Covid-19 examination.
{"title":"Sensitivitas Pemeriksaan Covid-19: Insulated Isothermal PCR (iiPCR) Dan Reverse Transcription PCR (RT-PCR)","authors":"Lisa Yunita, Dian Rachma Wijayanti, Apriani Riyanti","doi":"10.30651/jmlt.v6i1.12441","DOIUrl":"https://doi.org/10.30651/jmlt.v6i1.12441","url":null,"abstract":"Covid-19 (Corona Virus disease 2019) is a new type of disease caused by a virus from the Corona Virus group, namely SARS-CoV-2. Covid-19 may cause respiratory system disorders, ranging from mild symptoms such as flu to lung infections, such as pneumonia. Laboratory diagnoses for Covid-19 disease generally include hematological (complete blood examinations), and molecular or a combination of serology and molecular. PCR examination that can be carried out using the Insulated Isothermal PCR and Reverse Transcription PCR methods. This research is descriptive observational research. Data were collected at Grha Kedoya Hospital, West Jakarta, North Kedoya. The result showed a sensitivity of iiPCR score 82,6%, which is relatively lower than gold-standard RT-PCR. Our research suggested that RT-PCR is still an effective and sensitive method for Covid-19 examination.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135674339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Proteinuria indicates a kidney disorder and provides important prognostic information in diagnosing kidney disease. To determine the presence of protein in the urine, a urinalysis examination is carried out. Proteinuria examination generally uses the heating method with acetic acid, sulfosalicylic acid, and concentrated nitric acid as reagents. In Indonesia, the use of acetic acid for proteinuria examination is still the primary choice. An alternative reagent is needed that can replace the function of acetic acid. The objective of this study is to determine the potential of Averrhoa Blimbi juice as an alternative reagent for proteinuria examination.Methods: This was a Pre-experimental study with a completely randomized design. This study used urine samples from a patient with chronic kidney disease. Urine samples were examined using 6% acetic acid reagent as a control and using Averrhoa bilimbi juice with various concentrations. Each juice concentration was examined twice so that the experimental units in this study were 40 units. The Kruskal-Wallis test was used to determine the potential of Averrhoa bilimbi as an alternative reagent.Results: At concentrations of 5%-20% showed the same positive results as 6% acetic acid, namely +2, concentration 25%-35% resulted in +3, and other concentration resulted in +4. The results of data analysis showed p=0.04 (p<0.05). This shows that Averrhoa bilimbi has the potential as an alternative reagent for proteinuria examination.Conclusion: Averrhoa bilimbi juice with a 5%-20% concentration has the potential as an alternative reagent for 6% acetic acid to check proteinuria levels.Keywords: Averrhoa bilimbi ; alternative reagents; proteinuria; acetic acid; urinalysis
{"title":"The Potential of Averrhoa bilimbi Juice As An Alternative Reagent in Proteinuria Examination","authors":"Erna Kristinawati, Nutri Farda Nur, Idyatul Hasanah","doi":"10.30651/jmlt.v6i1.10604","DOIUrl":"https://doi.org/10.30651/jmlt.v6i1.10604","url":null,"abstract":"Background: Proteinuria indicates a kidney disorder and provides important prognostic information in diagnosing kidney disease. To determine the presence of protein in the urine, a urinalysis examination is carried out. Proteinuria examination generally uses the heating method with acetic acid, sulfosalicylic acid, and concentrated nitric acid as reagents. In Indonesia, the use of acetic acid for proteinuria examination is still the primary choice. An alternative reagent is needed that can replace the function of acetic acid. The objective of this study is to determine the potential of Averrhoa Blimbi juice as an alternative reagent for proteinuria examination.Methods: This was a Pre-experimental study with a completely randomized design. This study used urine samples from a patient with chronic kidney disease. Urine samples were examined using 6% acetic acid reagent as a control and using Averrhoa bilimbi juice with various concentrations. Each juice concentration was examined twice so that the experimental units in this study were 40 units. The Kruskal-Wallis test was used to determine the potential of Averrhoa bilimbi as an alternative reagent.Results: At concentrations of 5%-20% showed the same positive results as 6% acetic acid, namely +2, concentration 25%-35% resulted in +3, and other concentration resulted in +4. The results of data analysis showed p=0.04 (p<0.05). This shows that Averrhoa bilimbi has the potential as an alternative reagent for proteinuria examination.Conclusion: Averrhoa bilimbi juice with a 5%-20% concentration has the potential as an alternative reagent for 6% acetic acid to check proteinuria levels.Keywords: Averrhoa bilimbi ; alternative reagents; proteinuria; acetic acid; urinalysis","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79771483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
High exposure of cigarettes in Indonesia causes various disturbances in the body's metabolism, especially in the kidney, which is due to the cadmium metal content. Cadmium induces oxidative stress by the formation of free radicals and decrease natural antioxidant activity in the kidney organs (nephrotoxic). One way to overcome nephrotoxic by decreasing blood creatinine, we can utilize Moringa oleifera (MO) which is known as a "miracle plant" and have high contents of flavonoid groups, phenols, gallic acid, ascorbic acid and carotenoids as antioxidants and natural metal chelating agents. This research aimed to analyze the potential of Moringa leaves in decreasing cadmium and creatinine levels in the blood of white rats due to exposure of cigarette smoke (inhalation) at doses 200, 400 and 800 mg/kgwb. The type of research was an experimental study with quantitative analysis conducted in October 2021 – July 2022. The independent variable was the doses of Moringa leaves extract and the dependent variable were the blood cadmium and creatinine levels of white rats. The blood cadmium level was measured with AAS and the creatinine level was measured with Jaffe method without deproteination by photometry. The results of blood cadmium and creatinine levels in the treatment of MO extract at dose 200 mg/kgwb were 1.288875 μg/L and 0.85 mg/dL; MO extract at dose of 400 mg/kgwb were 0.811 μg/L and 0.775 mg/dL; MO extract at dose 800 mg /kgwb were 0.86275 μg/L and 0.7 mg/dL. Based on statistical analysis using Kruskal wallis, the results of the p value on cadmium levels in the blood were 0.004 < 0.05 and the p value on creatinine levels was 0.003 < 0.05, showed that there were significant differences in blood cadmium and creatinine levels in the treatment groups. Moringa leaf extract (Moringa oleifera) at a dose of 400 mg/kgwb had good potential in overcoming kidney damage in terms of creatinine levels due to cadmium exposure through cigarette smoke, which was equivalent to 44.3756 grams of fresh Moringa leaves that can be implemented for human as an additional antioxidant for overcoming free radicals. Keywords : Moringa oleifera, Cigarettes Smoke, Cadmium, Creatinine, Rattus norvegicus
{"title":"Potensi Daun Kelor (Moringa oleifera) Terhadap Kadar Kadmium Dan Kreatinin Dalam Darah Sebagai Indikator Kerusakan Fungsi Ginjal Pada Tikus Putih Yang Terpapar Asap Rokok","authors":"Christ Kartika Rahayuningsih, Riyan Agustin, Tacik Idayanti","doi":"10.30651/jmlt.v5i2.14261","DOIUrl":"https://doi.org/10.30651/jmlt.v5i2.14261","url":null,"abstract":"High exposure of cigarettes in Indonesia causes various disturbances in the body's metabolism, especially in the kidney, which is due to the cadmium metal content. Cadmium induces oxidative stress by the formation of free radicals and decrease natural antioxidant activity in the kidney organs (nephrotoxic). One way to overcome nephrotoxic by decreasing blood creatinine, we can utilize Moringa oleifera (MO) which is known as a \"miracle plant\" and have high contents of flavonoid groups, phenols, gallic acid, ascorbic acid and carotenoids as antioxidants and natural metal chelating agents. This research aimed to analyze the potential of Moringa leaves in decreasing cadmium and creatinine levels in the blood of white rats due to exposure of cigarette smoke (inhalation) at doses 200, 400 and 800 mg/kgwb. The type of research was an experimental study with quantitative analysis conducted in October 2021 – July 2022. The independent variable was the doses of Moringa leaves extract and the dependent variable were the blood cadmium and creatinine levels of white rats. The blood cadmium level was measured with AAS and the creatinine level was measured with Jaffe method without deproteination by photometry. The results of blood cadmium and creatinine levels in the treatment of MO extract at dose 200 mg/kgwb were 1.288875 μg/L and 0.85 mg/dL; MO extract at dose of 400 mg/kgwb were 0.811 μg/L and 0.775 mg/dL; MO extract at dose 800 mg /kgwb were 0.86275 μg/L and 0.7 mg/dL. Based on statistical analysis using Kruskal wallis, the results of the p value on cadmium levels in the blood were 0.004 < 0.05 and the p value on creatinine levels was 0.003 < 0.05, showed that there were significant differences in blood cadmium and creatinine levels in the treatment groups. Moringa leaf extract (Moringa oleifera) at a dose of 400 mg/kgwb had good potential in overcoming kidney damage in terms of creatinine levels due to cadmium exposure through cigarette smoke, which was equivalent to 44.3756 grams of fresh Moringa leaves that can be implemented for human as an additional antioxidant for overcoming free radicals. Keywords : Moringa oleifera, Cigarettes Smoke, Cadmium, Creatinine, Rattus norvegicus","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82408599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-03DOI: 10.30651/jmlt.v5i2.10623
Amalia Ovi Mustika Seno, Dita Pratiwi Kusuma Wardani, S. Supriyadi, Arif Mulyanto
Pulmonary tuberculosis is a disease caused by Mycobacterium tuberculosis. C-Reactive Protein (CRP) is produced in the body by the liver in response to inflammation caused by Mycobacterium tuberculosis infection. CRP as the main mediator of the non-specific immune system, and the concentration will increase to 1000 times higher than normal value in the event of tissue injury or infection. The aim of this study was to determine the comparison of CRP levels to the phase of anti-tuberculosis drug administration in pulmonary tuberculosis patients. The study was conducted by cross sectional study with a semi-quantitative method in June - July 2020 in Purwokerto Community Lung Health Center. The number of samples as much as 24 TB patients which is 12 TB patients in 0 month and 12 TB patients in 6 months. Data were analyzed by Mann Whitney Test. The mean rank in 0 month was 18.08 with median (min-max) 12 (12-24) mg / dL, whereas mean rank for 6 months was 6.92 with median (mix-max) was 0 (0- 12) mg / dL. Serum CRP levels in pulmonary TB patients in the 0-month treatment phase are higher than CRP levels in the 6-month treatment phase (p = 0,000).
{"title":"Perbandingan Kadar C-Reactive Protein Terhadap Fase Pemberian Obat Anti Tuberkulosis","authors":"Amalia Ovi Mustika Seno, Dita Pratiwi Kusuma Wardani, S. Supriyadi, Arif Mulyanto","doi":"10.30651/jmlt.v5i2.10623","DOIUrl":"https://doi.org/10.30651/jmlt.v5i2.10623","url":null,"abstract":"Pulmonary tuberculosis is a disease caused by Mycobacterium tuberculosis. C-Reactive Protein (CRP) is produced in the body by the liver in response to inflammation caused by Mycobacterium tuberculosis infection. CRP as the main mediator of the non-specific immune system, and the concentration will increase to 1000 times higher than normal value in the event of tissue injury or infection. The aim of this study was to determine the comparison of CRP levels to the phase of anti-tuberculosis drug administration in pulmonary tuberculosis patients. The study was conducted by cross sectional study with a semi-quantitative method in June - July 2020 in Purwokerto Community Lung Health Center. The number of samples as much as 24 TB patients which is 12 TB patients in 0 month and 12 TB patients in 6 months. Data were analyzed by Mann Whitney Test. The mean rank in 0 month was 18.08 with median (min-max) 12 (12-24) mg / dL, whereas mean rank for 6 months was 6.92 with median (mix-max) was 0 (0- 12) mg / dL. Serum CRP levels in pulmonary TB patients in the 0-month treatment phase are higher than CRP levels in the 6-month treatment phase (p = 0,000).","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90397143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-03DOI: 10.30651/jmlt.v5i2.12148
Wisnu Murti, D. R. Wijayanti, Wulan Fitriani Safari
SARS-Cov-2 is the newest coronavirus caused Covid-19 which can infect human and pandemic, COVID-19 parameter testing can be done with serological tests used to detect antibody or antigen and also molecular test using PCR method. This research is aimed to compare the sensitivity and specificity between COVID-19 Ag rapid test and PCR test. This research is an analytical descriptive study with 421 samples were obtained from documentation study with probability systematic random sampling and by using secondary data taken from Laboratory Information System (LIS) at Siloam Hospitals Lippo Village Tangerang and the type of observational analytical research with cross sectional design. The analysis results show the sensitivity rate was 96.64%, specificity was 93.75%, PPV was 89.44%, NPV was 98.08%, LR (+) was 15.46, and LR (-) was 0.04. The conclusion of the research result, COVID-19 Ag rapid test is valid to be used as a diagnostic test because it has high rate of sensitivity and specificity according to WHO regulations.
{"title":"Analisis Sensitivitas Dan Spesifisitas COVID-19 Ag Rapid Test Terhadap PCR Test","authors":"Wisnu Murti, D. R. Wijayanti, Wulan Fitriani Safari","doi":"10.30651/jmlt.v5i2.12148","DOIUrl":"https://doi.org/10.30651/jmlt.v5i2.12148","url":null,"abstract":"SARS-Cov-2 is the newest coronavirus caused Covid-19 which can infect human and pandemic, COVID-19 parameter testing can be done with serological tests used to detect antibody or antigen and also molecular test using PCR method. This research is aimed to compare the sensitivity and specificity between COVID-19 Ag rapid test and PCR test. This research is an analytical descriptive study with 421 samples were obtained from documentation study with probability systematic random sampling and by using secondary data taken from Laboratory Information System (LIS) at Siloam Hospitals Lippo Village Tangerang and the type of observational analytical research with cross sectional design. The analysis results show the sensitivity rate was 96.64%, specificity was 93.75%, PPV was 89.44%, NPV was 98.08%, LR (+) was 15.46, and LR (-) was 0.04. The conclusion of the research result, COVID-19 Ag rapid test is valid to be used as a diagnostic test because it has high rate of sensitivity and specificity according to WHO regulations. ","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90049739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-03DOI: 10.30651/jmlt.v5i2.14419
Berlian Rustantina, D. Wahyuni, Kamalia Fikri, Nimatuzahroh Nimatuzahroh, Laily Ainun Jaiyah, Aisyah Rahmawati, Hesti Nurhayati, Nur Romadhon
Aedes aegypti merupakan spesies nyamuk dari genus Aedes yang berlimpah sepanjang tahun, terutama pada musim hujan. Aedes aegypti dapat hidup di lingkungan yang ekstrim, sehingga keberadaan Aedes aegypti tersebar merata di daerah tropis dan subtropis. Aedes aegypti merupakan vektor pembawa penyakit Demam Berdarah Dengue (DBD) pada manusia. Penyakit menular berbasis vektor di beberapa wilayah Indonesia menjadi Kejadian Luar Biasa (KLB) dengan angka kematian yang cukup tinggi. Salah satu upaya pengendaliannya sangat penting, sehingga dapat menekan pertumbuhan vektor demam berdarah. Pengendalian yang paling baik dan ramah lingkungan adalah dengan menggunakan bioinsektisida. Salah satu tanaman yang dapat digunakan untuk bioinsektisida adalah mangga (Mangifera indica L.). Penelitian eksperimental ini menggunakan metode ekstraksi dengan pelarut etanol 96%. Hasil uji pendahuluan diperoleh LC5 sebesar 500 ppm dan LC95 sebesar 2.500 ppm. LC5 dan LC95 merupakan langkah awal untuk menentukan konsentrasi ekstrak yang akan digunakan pada pengujian akhir. Konsentrasi serial yang digunakan untuk pengujian akhir adalah: 500ppm, 1,000ppm, 1,500ppm, 2,000ppm, 2,500ppm. Hasil pengujian akhir dianalisis untuk mendapatkan nilai LC50, yaitu 1.406,36 ppm, dengan batas bawah 1.282,97 ppm dan batas atas 1.518,52 ppm. LC50 digunakan untuk menentukan konsentrasi ekstrak yang dapat membunuh 50% larva. Kata kunci: Demam berdarah, Aedes aegypti, Mortalitas, Toksisitas, Ekstrak.
{"title":"Lethal Concentration (LC50) Ekstrak Kulit Buah Mangga (Mangifera indica L.) Varietas Gadung Terhadap Mortalitas Larva Nyamuk Aedes aegypti L. Sebagai Bioinsektisida Baru","authors":"Berlian Rustantina, D. Wahyuni, Kamalia Fikri, Nimatuzahroh Nimatuzahroh, Laily Ainun Jaiyah, Aisyah Rahmawati, Hesti Nurhayati, Nur Romadhon","doi":"10.30651/jmlt.v5i2.14419","DOIUrl":"https://doi.org/10.30651/jmlt.v5i2.14419","url":null,"abstract":"Aedes aegypti merupakan spesies nyamuk dari genus Aedes yang berlimpah sepanjang tahun, terutama pada musim hujan. Aedes aegypti dapat hidup di lingkungan yang ekstrim, sehingga keberadaan Aedes aegypti tersebar merata di daerah tropis dan subtropis. Aedes aegypti merupakan vektor pembawa penyakit Demam Berdarah Dengue (DBD) pada manusia. Penyakit menular berbasis vektor di beberapa wilayah Indonesia menjadi Kejadian Luar Biasa (KLB) dengan angka kematian yang cukup tinggi. Salah satu upaya pengendaliannya sangat penting, sehingga dapat menekan pertumbuhan vektor demam berdarah. Pengendalian yang paling baik dan ramah lingkungan adalah dengan menggunakan bioinsektisida. Salah satu tanaman yang dapat digunakan untuk bioinsektisida adalah mangga (Mangifera indica L.). Penelitian eksperimental ini menggunakan metode ekstraksi dengan pelarut etanol 96%. Hasil uji pendahuluan diperoleh LC5 sebesar 500 ppm dan LC95 sebesar 2.500 ppm. LC5 dan LC95 merupakan langkah awal untuk menentukan konsentrasi ekstrak yang akan digunakan pada pengujian akhir. Konsentrasi serial yang digunakan untuk pengujian akhir adalah: 500ppm, 1,000ppm, 1,500ppm, 2,000ppm, 2,500ppm. Hasil pengujian akhir dianalisis untuk mendapatkan nilai LC50, yaitu 1.406,36 ppm, dengan batas bawah 1.282,97 ppm dan batas atas 1.518,52 ppm. LC50 digunakan untuk menentukan konsentrasi ekstrak yang dapat membunuh 50% larva. Kata kunci: Demam berdarah, Aedes aegypti, Mortalitas, Toksisitas, Ekstrak. ","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75767671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-03DOI: 10.30651/jmlt.v5i2.14103
I. W. T. Aryasa, N. P. Artini
Vinegar has the chemical name acetic acid derived from the Latin word acetum, acetic acid, ethanoic acid, or vinegar is an organic acid chemical compound that is the most important carboxylic acid in commerce, industry, and laboratories known as a sour taster. and aroma in food. But keep in mind that vinegar can work as a disinfectant. One type of vinegar that we know is apple cider vinegar. Based on the background that has been stated above, the problem formulated in this study is how apple cider vinegar inhibits the growth of Escherichia coli and Staphylococcus aureus bacteria. The analysis of this research is in the form of a quasi-experimental, namely by comparing the inhibitory power of apple cider vinegar and control on the growth of Escherichia coli and Staphylococcus aureus bacteria and post-test only control group design. Based on the results of the research that has been carried out, the results show that the diameter of the inhibition zone formed on Escherichia coli bacteria for foreign apple cider vinegar, Indonesian apple cider vinegar, and local apple cider vinegar is 12.33 ± 0.12 mm, 2.21 ± 0.12 mm and 7.42 ± 0.09 mm. While the diameter of the inhibition zone formed on Staphylococcus aureus bacteria for foreign apple cider vinegar, Indonesian apple cider vinegar, and local apple cider vinegar was 14.56 ± 0.46 mm, 0.00 ± 0.00 mm, and 10.36 ± 0.27 mm. So it can be concluded that apple cider vinegar has antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria.
{"title":"Antibakteri Cuka Apel Terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus Secara In Vitro","authors":"I. W. T. Aryasa, N. P. Artini","doi":"10.30651/jmlt.v5i2.14103","DOIUrl":"https://doi.org/10.30651/jmlt.v5i2.14103","url":null,"abstract":"Vinegar has the chemical name acetic acid derived from the Latin word acetum, acetic acid, ethanoic acid, or vinegar is an organic acid chemical compound that is the most important carboxylic acid in commerce, industry, and laboratories known as a sour taster. and aroma in food. But keep in mind that vinegar can work as a disinfectant. One type of vinegar that we know is apple cider vinegar. Based on the background that has been stated above, the problem formulated in this study is how apple cider vinegar inhibits the growth of Escherichia coli and Staphylococcus aureus bacteria. The analysis of this research is in the form of a quasi-experimental, namely by comparing the inhibitory power of apple cider vinegar and control on the growth of Escherichia coli and Staphylococcus aureus bacteria and post-test only control group design. Based on the results of the research that has been carried out, the results show that the diameter of the inhibition zone formed on Escherichia coli bacteria for foreign apple cider vinegar, Indonesian apple cider vinegar, and local apple cider vinegar is 12.33 ± 0.12 mm, 2.21 ± 0.12 mm and 7.42 ± 0.09 mm. While the diameter of the inhibition zone formed on Staphylococcus aureus bacteria for foreign apple cider vinegar, Indonesian apple cider vinegar, and local apple cider vinegar was 14.56 ± 0.46 mm, 0.00 ± 0.00 mm, and 10.36 ± 0.27 mm. So it can be concluded that apple cider vinegar has antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84899735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRAKCandida albicans merupakan anggota flora normal yang terdapat pada selaput mukosa, saluran pencernaan, saluran pernafasan, uretra, vagina, kulit dan kuku tangan dan kaki. Candida albicans dapat tumbuh pada berbagai macam pH, tetapi umumnya pertumbuhan akan lebih baik pada pH antara 4,5-6,5. Candida albicans dapat tumbuh dalam perbenihan pada suhu 28ºC – 37ºC. Jenis penelitian yang digunakan merupakan penelitian deskriptif kuantitatif. Penelitian ini bertujuan untuk untuk mengetahui adanya jamur Candida albicans pada urine penderita infeksi saluran kemih menggunakan metode Real Time PCR. Populasi dalam penelitian penderita infeksi saluran kemih. Infeksi saluran kemih merupakan infeksi keadaan klinis akibat berkembangbiaknya mikroorganisme yang menyebabkan inflamasi pada saluran kemih. Penelitian dilaksanakan di Laboratorium Parasitologi dan Laboratorium Biologi Molekuler pada bulan April 2022 hingga Mei 2022. Pada penelitian ini dilakukan beberapa tahapan pemeriksaan seperti isolasi kultur pada media Sabaroud Dextrose Agar, pemeriksaan makroskopis dan mikroskopis, pembuatan suspensi sel, uji ekstraksi, uji kemurnian DNA, optimasi dan pemeriksaan Real Time PCR dengan menunjukkan hasil berupa CT. Hasil penelitian menunjukkan pada pemeriksaan Real Time PCR ini didapatkan sejumlah 2 sampel dengan persentase 22,2% pada kode sampel S7 dengan nilai CT 28,50 dan kode sampel S9 dengan nilai CT 23,19 dan hasil negatif sejumlah 7 sampel dengan persentase 77,8%. Adapun faktor penyebab yang dapat mengakibatkan keberadaan jamur Candida albicans pada urine penderita infeksi saluran kemih seperti personal hygiene, penderita diabetes mellitus, alat kontrasepsi dan penggunaan antiseptik.
{"title":"Deteksi Jamur Candida albicans Pada Urine Penderita Infeksi Saluran Kemih Menggunakan Metode RT-PCR","authors":"Retno Sasongkowati, Anita Dwi Anggraini, Dellanis Arina Putri","doi":"10.30651/jmlt.v5i2.14409","DOIUrl":"https://doi.org/10.30651/jmlt.v5i2.14409","url":null,"abstract":" ABSTRAKCandida albicans merupakan anggota flora normal yang terdapat pada selaput mukosa, saluran pencernaan, saluran pernafasan, uretra, vagina, kulit dan kuku tangan dan kaki. Candida albicans dapat tumbuh pada berbagai macam pH, tetapi umumnya pertumbuhan akan lebih baik pada pH antara 4,5-6,5. Candida albicans dapat tumbuh dalam perbenihan pada suhu 28ºC – 37ºC. Jenis penelitian yang digunakan merupakan penelitian deskriptif kuantitatif. Penelitian ini bertujuan untuk untuk mengetahui adanya jamur Candida albicans pada urine penderita infeksi saluran kemih menggunakan metode Real Time PCR. Populasi dalam penelitian penderita infeksi saluran kemih. Infeksi saluran kemih merupakan infeksi keadaan klinis akibat berkembangbiaknya mikroorganisme yang menyebabkan inflamasi pada saluran kemih. Penelitian dilaksanakan di Laboratorium Parasitologi dan Laboratorium Biologi Molekuler pada bulan April 2022 hingga Mei 2022. Pada penelitian ini dilakukan beberapa tahapan pemeriksaan seperti isolasi kultur pada media Sabaroud Dextrose Agar, pemeriksaan makroskopis dan mikroskopis, pembuatan suspensi sel, uji ekstraksi, uji kemurnian DNA, optimasi dan pemeriksaan Real Time PCR dengan menunjukkan hasil berupa CT. Hasil penelitian menunjukkan pada pemeriksaan Real Time PCR ini didapatkan sejumlah 2 sampel dengan persentase 22,2% pada kode sampel S7 dengan nilai CT 28,50 dan kode sampel S9 dengan nilai CT 23,19 dan hasil negatif sejumlah 7 sampel dengan persentase 77,8%. Adapun faktor penyebab yang dapat mengakibatkan keberadaan jamur Candida albicans pada urine penderita infeksi saluran kemih seperti personal hygiene, penderita diabetes mellitus, alat kontrasepsi dan penggunaan antiseptik. ","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82756091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Examination of levels of C-Reactive Protein (CRP) in the blood is one of the tests. that can detect inflammation at an early stage. C-Reactive Protein (CRP) is an inflammatory marker that is synthesized in the liver. CRP levels increase due to trauma, bacterial infection and inflammation (inflammation and tissue damage). CRP is synthesized by the liver and It released into the bloodstream for 6-10 hours after the acute inflammatory process and acute destruction. Tissue detection CRP is also one of several proteins often referred to as the acute phase and It is used to monitor changes in the acute inflammatory phase associated with many infectious and autoimmune diseases. Examination of C-Reactive Protein (CRP) used serum and plasma samples of K3EDTA. C-Reactive Protein levels were measured using the Immunoturbidimetric method. This type of research was cross sectional. The study was conducted at the Clinical Pathology Laboratory, Faculty of Science and Technology, Institute of Health Sciences and Technology Muhammadiyah Palembang on December 21, 2021 with samples were 30. The results of the study had an average value in serum samples of 6.3 mg/L and an average value in plasma. K3EDTA 6.5 mg/L. Wilcoxon test results obtained p value = 0.000. The conclusion of the study was that there were differences in the examination of C-Reactive Protein (CRP) levels in serum and plasma samples of K3EDTA using the immunoturbidimetric method.
{"title":"Perbedaan Kadar C-Reaktive Protein (CRP) Pada Sampel Serum dan Plasma K3EDTA Dengan Metode Imunoturbidimetri","authors":"Helniasari Helniasari, Nurhidayanti Nurhidayanti, Bastian Bastian","doi":"10.30651/jmlt.v5i2.13350","DOIUrl":"https://doi.org/10.30651/jmlt.v5i2.13350","url":null,"abstract":"Examination of levels of C-Reactive Protein (CRP) in the blood is one of the tests. that can detect inflammation at an early stage. C-Reactive Protein (CRP) is an inflammatory marker that is synthesized in the liver. CRP levels increase due to trauma, bacterial infection and inflammation (inflammation and tissue damage). CRP is synthesized by the liver and It released into the bloodstream for 6-10 hours after the acute inflammatory process and acute destruction. Tissue detection CRP is also one of several proteins often referred to as the acute phase and It is used to monitor changes in the acute inflammatory phase associated with many infectious and autoimmune diseases. Examination of C-Reactive Protein (CRP) used serum and plasma samples of K3EDTA. C-Reactive Protein levels were measured using the Immunoturbidimetric method. This type of research was cross sectional. The study was conducted at the Clinical Pathology Laboratory, Faculty of Science and Technology, Institute of Health Sciences and Technology Muhammadiyah Palembang on December 21, 2021 with samples were 30. The results of the study had an average value in serum samples of 6.3 mg/L and an average value in plasma. K3EDTA 6.5 mg/L. Wilcoxon test results obtained p value = 0.000. The conclusion of the study was that there were differences in the examination of C-Reactive Protein (CRP) levels in serum and plasma samples of K3EDTA using the immunoturbidimetric method.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90813621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-03DOI: 10.30651/jmlt.v5i2.15518
Anak Agung Lilyk Cahyani, Putu Ayu Parwati
Kesalahan tahap pra-analitik memberikan kontribusi paling besar pada kesalahan laboratorium. Kesalahan pada tahap pra-analitik yang sering terjadi adalah hemolisis (53,2%), volume spesimen kurang (7,5%), tulisan tangan yang tidak bisa dibaca (7,1%), kesalahan identifikasi pasien, ada bekuan, vacum container yang salah/antikoagulan, volume antikoagulan yang tidak sesuai, spesimen diambil dari jalur infus, dan kesalahan waktu dalam pengambilan spesimen. Penelitian dilakukan untuk menganalisis tahapan pengambilan dan pengelolaan spesimen darah di laboratorium RSUD Wangaya Denpasar. Teknik pengambilan sampel dilakukan secara total sampling sebanyak 18 orang petugas ATLM di laboratorium Patologi Klinik. Data primer dikumpulkan dari hasil wawancara dan kuisioner untuk menganalisis pengetahuan terkait pengambilan dan pengelolaan spesimen darah. Hasil dengan skor tertinggi dan dalam kategori sangat baik yaitu pada pengambilan spesimen (97,8%). Skor terendah dan dalam kategori baik adalah pemberian identitas pasien (82,5%). Petugas laboratorium di RSUD Wangaya telah melakukan tahapan pengambilan dan pengelolaan spesimen darah sesuai peraturan Menteri Kesehatan RI tahun 2013 dan masuk dalam kategori sangat baik yaitu 91,48%.
{"title":"Manajemen Pengambilan dan Pengelolaan Spesimen Darah di Laboratorium RSUD Wangaya Denpasar","authors":"Anak Agung Lilyk Cahyani, Putu Ayu Parwati","doi":"10.30651/jmlt.v5i2.15518","DOIUrl":"https://doi.org/10.30651/jmlt.v5i2.15518","url":null,"abstract":"Kesalahan tahap pra-analitik memberikan kontribusi paling besar pada kesalahan laboratorium. Kesalahan pada tahap pra-analitik yang sering terjadi adalah hemolisis (53,2%), volume spesimen kurang (7,5%), tulisan tangan yang tidak bisa dibaca (7,1%), kesalahan identifikasi pasien, ada bekuan, vacum container yang salah/antikoagulan, volume antikoagulan yang tidak sesuai, spesimen diambil dari jalur infus, dan kesalahan waktu dalam pengambilan spesimen. Penelitian dilakukan untuk menganalisis tahapan pengambilan dan pengelolaan spesimen darah di laboratorium RSUD Wangaya Denpasar. Teknik pengambilan sampel dilakukan secara total sampling sebanyak 18 orang petugas ATLM di laboratorium Patologi Klinik. Data primer dikumpulkan dari hasil wawancara dan kuisioner untuk menganalisis pengetahuan terkait pengambilan dan pengelolaan spesimen darah. Hasil dengan skor tertinggi dan dalam kategori sangat baik yaitu pada pengambilan spesimen (97,8%). Skor terendah dan dalam kategori baik adalah pemberian identitas pasien (82,5%). Petugas laboratorium di RSUD Wangaya telah melakukan tahapan pengambilan dan pengelolaan spesimen darah sesuai peraturan Menteri Kesehatan RI tahun 2013 dan masuk dalam kategori sangat baik yaitu 91,48%.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91089979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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