Objective: Epigenetic modulation of gene expression by DNA promoter methylation may contribute to acquired resistance to chemotherapy in cancer cells. Decitabine (5-aza-2'- deoxycytidine), a demethylating agent, may act synergistically with standard chemotherapy regimens to activate epigenetically silenced genes. In the present in vitro study, it was investigated the effect of gene methylation level after treatment with decitabine and combination of decitabine with anthracycline-based therapeutics (5-fluorouracil plus epirubicine plus cyclophosphamide; FEC) on breast cancer cells (MCF-7 and MDA-MB-231). Methods: The effect of decitabine and its combination with FEC on different genes methylation level has been tested in MDA-MB-231 and MCF-7 human breast cancer cell lines. The effect of decitabine on the cell viability was assayed by MTT assay. Methylight real-time PCR and methylation specific PCR were carried out to determine the methylation status of certain genes: DAPK, TMS1, MGMT and the global methylation marker LINE-1. Results: The LINE-1 methylation status significantly decreased in both cell lines after treatment with the combination of decitabine with FEC. In MDA-MB-231 cells, methylation of the TMS1 and the MGMT gene promoter was significantly reduced by FEC plus decitabine while no effect was observed in MCF-7 cells. Conclusion: Anthracycline-based therapy regimens in combination with demethylating agents such as decitabine may affect chemotherapy outcome by modulation of apoptosis- relevant genes by methylation. More importantly, this modulation seems to be dependent on
{"title":"Changes in Gene Methylation Following Chemotherapy in Breast Cancer Cell Lines","authors":"F. Ari, R. Napieralski, E. Ulukaya","doi":"10.5505/TJB.2013.46320","DOIUrl":"https://doi.org/10.5505/TJB.2013.46320","url":null,"abstract":"Objective: Epigenetic modulation of gene expression by DNA promoter methylation may contribute to acquired resistance to chemotherapy in cancer cells. Decitabine (5-aza-2'- deoxycytidine), a demethylating agent, may act synergistically with standard chemotherapy regimens to activate epigenetically silenced genes. In the present in vitro study, it was investigated the effect of gene methylation level after treatment with decitabine and combination of decitabine with anthracycline-based therapeutics (5-fluorouracil plus epirubicine plus cyclophosphamide; FEC) on breast cancer cells (MCF-7 and MDA-MB-231). Methods: The effect of decitabine and its combination with FEC on different genes methylation level has been tested in MDA-MB-231 and MCF-7 human breast cancer cell lines. The effect of decitabine on the cell viability was assayed by MTT assay. Methylight real-time PCR and methylation specific PCR were carried out to determine the methylation status of certain genes: DAPK, TMS1, MGMT and the global methylation marker LINE-1. Results: The LINE-1 methylation status significantly decreased in both cell lines after treatment with the combination of decitabine with FEC. In MDA-MB-231 cells, methylation of the TMS1 and the MGMT gene promoter was significantly reduced by FEC plus decitabine while no effect was observed in MCF-7 cells. Conclusion: Anthracycline-based therapy regimens in combination with demethylating agents such as decitabine may affect chemotherapy outcome by modulation of apoptosis- relevant genes by methylation. More importantly, this modulation seems to be dependent on","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"152 1","pages":"154-162"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73990593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Aslan, N. Handayani, Erythrina Stavila, K. Loos
Objective: In this study, the conditions of covalent immobilization of Pseudomonas fluorescens lipase onto an oxirane-activated support (Amberzyme) were optimized to obtain a high activity yield. Furthermore, the operational and storage stabilities of immobilized lipase were tested. Methods: Optimum conditions for immobilization were determined by changing individually the conditions (pH from 5 to 9; buffer concentration from 0.025 to 2.5 M; amount of Amberzyme from 100 to 500 mg and duration of immobilization from 24 to 120 h). Amounts of protein and the activity of enzyme were determined by UV/Vis (PYE UNICAM SP8-200 UV/Vis spectrophotometer). Results: Immobilization conditions (pH and molar concentration of immobilization buffer, enzyme/support ratio and immobilization duration) significantly affected the immobilization efficiency. 100% immobilization yield and 145% activity yield were achieved by optimizing the immobilization conditions. Operational and storage stabilities of immobilized lipase were determined as well. The immobilized enzymes retained its activity for 20 consecutive batch reactions. Furthermore, the immobilized lipase showed a high storage stability as no decrease in its activity was observed for 20 days. Conclusion: Our results obtained in the present study are the best in the covalent immobilization of Pseudomonas fluorescens lipase in the literature. Therefore our future studies will focus on using the immobilized Pseudomonas fluorescens lipase for the production of biodiesel, hydrolysis of oils and various important esterification reactions.
{"title":"Improved Performance of Pseudomonas fluorescens lipase by covalent immobilization onto Amberzyme","authors":"Y. Aslan, N. Handayani, Erythrina Stavila, K. Loos","doi":"10.5505/TJB.2013.30085","DOIUrl":"https://doi.org/10.5505/TJB.2013.30085","url":null,"abstract":"Objective: In this study, the conditions of covalent immobilization of Pseudomonas fluorescens lipase onto an oxirane-activated support (Amberzyme) were optimized to obtain a high activity yield. Furthermore, the operational and storage stabilities of immobilized lipase were tested. Methods: Optimum conditions for immobilization were determined by changing individually the conditions (pH from 5 to 9; buffer concentration from 0.025 to 2.5 M; amount of Amberzyme from 100 to 500 mg and duration of immobilization from 24 to 120 h). Amounts of protein and the activity of enzyme were determined by UV/Vis (PYE UNICAM SP8-200 UV/Vis spectrophotometer). Results: Immobilization conditions (pH and molar concentration of immobilization buffer, enzyme/support ratio and immobilization duration) significantly affected the immobilization efficiency. 100% immobilization yield and 145% activity yield were achieved by optimizing the immobilization conditions. Operational and storage stabilities of immobilized lipase were determined as well. The immobilized enzymes retained its activity for 20 consecutive batch reactions. Furthermore, the immobilized lipase showed a high storage stability as no decrease in its activity was observed for 20 days. Conclusion: Our results obtained in the present study are the best in the covalent immobilization of Pseudomonas fluorescens lipase in the literature. Therefore our future studies will focus on using the immobilized Pseudomonas fluorescens lipase for the production of biodiesel, hydrolysis of oils and various important esterification reactions.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"143 1","pages":"313-318"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84197355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The objective of present study was to evaluate the antibacterial and antioxidant activities of essential oils from Thymus vulgaris, Thymus kotschyanus, Ziziphora tenuior and Ziziphora clinopodioides. Methods: The antioxidant potency of essential oils was determined by 2,2-diphenyl-1- picrylhydrazyl and reducing power assays. Total phenolic contents of essential oils were determined using the Folin-Ciocalteu reagent assay. The antibacterial activity of essential oils was evaluated using agar disc diffusion and minimum inhibitory concentration (MIC) methods. Results: The essential oils of Ziziphora clinopodioides and Thymus vulgaris showed the highest antioxidant activity. The essential oils of Thymus vulgaris showed the strongest antibacterial activity with the widest inhibition zone and the lowest MIC value (2.5 μl/ml). The essential oils of Thymus vulgaris had the highest concentration of total phenolics (116.5
{"title":"In vitro antioxidant and antibacterial properties and total phenolic contents of essential oils from Thymus vulgaris, T. kotschyanus, Ziziphora tenuior and Z. clinopodioides","authors":"J. Aliakbarlu, Farnaz Shameli","doi":"10.5505/TJB.2013.58070","DOIUrl":"https://doi.org/10.5505/TJB.2013.58070","url":null,"abstract":"Objective: The objective of present study was to evaluate the antibacterial and antioxidant activities of essential oils from Thymus vulgaris, Thymus kotschyanus, Ziziphora tenuior and Ziziphora clinopodioides. Methods: The antioxidant potency of essential oils was determined by 2,2-diphenyl-1- picrylhydrazyl and reducing power assays. Total phenolic contents of essential oils were determined using the Folin-Ciocalteu reagent assay. The antibacterial activity of essential oils was evaluated using agar disc diffusion and minimum inhibitory concentration (MIC) methods. Results: The essential oils of Ziziphora clinopodioides and Thymus vulgaris showed the highest antioxidant activity. The essential oils of Thymus vulgaris showed the strongest antibacterial activity with the widest inhibition zone and the lowest MIC value (2.5 μl/ml). The essential oils of Thymus vulgaris had the highest concentration of total phenolics (116.5","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"29 1","pages":"425-431"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86512653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Ozkan, F. Erkoç, H. Çelik, A. Dinçel, B. Şimşek, L. Kayrın, Subhan Ekşioğlu, M. Yüksel, G. Haklar, Özlem Yavuz, S. Kurban, H. Uysal, U. Kisa, M. Konuk, E. Bodur, M. Selvi, G. Akça
{"title":"The Multidisciplinary Approach to Biochemistry Laboratory Education","authors":"Y. Ozkan, F. Erkoç, H. Çelik, A. Dinçel, B. Şimşek, L. Kayrın, Subhan Ekşioğlu, M. Yüksel, G. Haklar, Özlem Yavuz, S. Kurban, H. Uysal, U. Kisa, M. Konuk, E. Bodur, M. Selvi, G. Akça","doi":"10.5505/TJB.2013.02360","DOIUrl":"https://doi.org/10.5505/TJB.2013.02360","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"4 1","pages":"506-512"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88803301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To determine tandem repeat polymorphism and heteroplasmy in three sturgeon species (A. stellatus, A. gueldenstaedtii and H. huso) from the Turkish coast of Black Sea. Methods: tRNApro and D-loop segment of mtDNA from three sturgeon species were amplified via PCR and sequenced. For each species, homoplasmic individuals with different product lengths, repeat motifs and regions were determined and the repeat numbers and frequencies were calculated. The variation in mtDNA size present in the overall sample of sturgeon from Turkish waters was apportioned into hierarchical components. Also, the statistical approach described by other researchers was used for the calculation of inter- species and intra-species genetic variation. Results: The results showed that all three species reveal 2-6 copies of different mtDNA length variants attributable to varying copy numbers of an 82-84bp repeat sequences. A total of 9.9% of the sturgeons were heteroplasmic, bearing three to five repeat variants. The highest number of observed repeat units rate was 45.8% in 3 repeats morph in A. gueldenstaedtii. The mean genetic diversity within individuals (Kb) was higher in A. gueldenstaedtii and A. stellatus than in H. huso (0.625, 0.620, and 0.500, respectively). Conclusion: The repeat region, responsible for length variations and heteroplasmy, is located near the end of the D-loop and control region separated by only a few nucleotides from the tRNApro gene.
目的:测定黑海土耳其海岸三种鲟(A. stellatus, A. gueldenstaedtii和H. huso)的串联重复序列多态性和异质性。方法:对3种鲟的mtDNA tRNApro和d环片段进行PCR扩增和测序。对每个物种确定具有不同产物长度、重复基序和区域的同质个体，并计算重复次数和频率。在mtDNA大小的变化存在于从土耳其水域鲟鱼的整体样本被分配到分层的组成部分。此外，还采用了其他研究人员描述的统计方法来计算种间和种内遗传变异。结果:由于82 ~ 84bp重复序列拷贝数的不同，3个物种均存在2 ~ 6个不同mtDNA长度的变异。总共9.9%的鲟鱼是异质的，有3到5个重复变异。在3个重复序列中观察到的重复单位率最高，为45.8%。个体内遗传多样性的平均值(Kb)分别为0.625、0.620和0.500，居群和居群的平均值(Kb)高于居群。结论:负责长度变异和异质性的重复区域位于d环末端附近，与tRNApro基因仅隔几个核苷酸的控制区。
{"title":"Heteroplasmy and length variation in the tRNApro- Dloop regions of three sturgeon species (A. stellatus, A. gueldenstaedtii and H. huso) from the Turkish coast of the Black Sea","authors":"Y. Çi̇ftçi, O. Eroğlu, Ş. Firidin","doi":"10.5505/TJB.2013.70783","DOIUrl":"https://doi.org/10.5505/TJB.2013.70783","url":null,"abstract":"Objective: To determine tandem repeat polymorphism and heteroplasmy in three sturgeon species (A. stellatus, A. gueldenstaedtii and H. huso) from the Turkish coast of Black Sea. Methods: tRNApro and D-loop segment of mtDNA from three sturgeon species were amplified via PCR and sequenced. For each species, homoplasmic individuals with different product lengths, repeat motifs and regions were determined and the repeat numbers and frequencies were calculated. The variation in mtDNA size present in the overall sample of sturgeon from Turkish waters was apportioned into hierarchical components. Also, the statistical approach described by other researchers was used for the calculation of inter- species and intra-species genetic variation. Results: The results showed that all three species reveal 2-6 copies of different mtDNA length variants attributable to varying copy numbers of an 82-84bp repeat sequences. A total of 9.9% of the sturgeons were heteroplasmic, bearing three to five repeat variants. The highest number of observed repeat units rate was 45.8% in 3 repeats morph in A. gueldenstaedtii. The mean genetic diversity within individuals (Kb) was higher in A. gueldenstaedtii and A. stellatus than in H. huso (0.625, 0.620, and 0.500, respectively). Conclusion: The repeat region, responsible for length variations and heteroplasmy, is located near the end of the D-loop and control region separated by only a few nucleotides from the tRNApro gene.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"9 1","pages":"250-257"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88916482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Karl Landsteiner was one of the first scientists to study the processes of immunity and is known as the founder of serology. He discovered that there are different groups of human blood and established the ABO-system based on haemagglutination. This blood grouping made blood transfusion routine medical practice. In 1930, he was awarded the Nobel prize in physiology or medicine for his discovery of human blood groups.This paper provides an overview on the discovery of the blood grouping and the physician behind this discovery, Karl Landsteiner , through philately.
{"title":"Medicine in philately, Karl Landsteiner: the father of blood grouping","authors":"E. E. V. Lutz, A. Ataman","doi":"10.5505/TJB.2013.73644","DOIUrl":"https://doi.org/10.5505/TJB.2013.73644","url":null,"abstract":"Karl Landsteiner was one of the first scientists to study the processes of immunity and is known as the founder of serology. He discovered that there are different groups of human blood and established the ABO-system based on haemagglutination. This blood grouping made blood transfusion routine medical practice. In 1930, he was awarded the Nobel prize in physiology or medicine for his discovery of human blood groups.This paper provides an overview on the discovery of the blood grouping and the physician behind this discovery, Karl Landsteiner , through philately.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"67 1","pages":"176-180"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87030797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Optimization of dilute acid and alkaline peroxide pretreatment to enhance bioethanol production from wheat straw by co-fermentation","authors":"Pınar Karagöz, M. Özkan","doi":"10.5505/TJB.2013.57431","DOIUrl":"https://doi.org/10.5505/TJB.2013.57431","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"16 1","pages":"457-467"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87248170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: In this study, it was aimed to investigate the changing levels of antioxidants along with aging. Malondialdehyde (MDA), total antioxidant (TAS) levels, and paraoxanase (PON) and arylesterase enzyme activities were examined as well as Coenzyme Q10, that has an immense role in production of ATP and, in fact, is a potent antioxidant with a lipophillic structure Methods: Research included 160 men and women whose ages differ from 20 to 70 years old and also live in Gaziantep area. Research was done by dividing subjects into 3 age groups: 50 people belonged to 20–30, 75 people to 31–50 and 35 people to 51–70 age group. Groups were constituted from people who are non-obese and who do not have any physical complaints. In taken plasma samples coenzyme Q10, MDA and TAS levels, PON and arylesterase enzyme activities were measured. The measurements were performed as follows: MDA by using thiobarbituric acid method, TAS, PON and arylesterase with spectrophotometric methods, and coenzyme Q10 with HPLC method were measured. Results: A correlation was found between age and coenzyme Q10 in our study (p<0.001). Coenzyme Q10 was found to be increased with aging. Statistically significant and negative linear relationship was found between coenzyme Q10, MDA levels, and PON and arylesterase activities (p<0.01, p<0.001). Conclusion: Despite the aging, the antioxidant system in healthy people works to protect the body from the endogenous or exogenous oxidants. However, levels of antioxidants cannot compansate with the rising oxidant levels in case of an illness. Aging is not a disease itself, but the process of aging is accelerated by a disease.
{"title":"The relationship of antioxidants with aging","authors":"I. Geyikli, M. Akan, M. Tarakçıoğlu","doi":"10.5505/TJB.2013.21939","DOIUrl":"https://doi.org/10.5505/TJB.2013.21939","url":null,"abstract":"Objective: In this study, it was aimed to investigate the changing levels of antioxidants along with aging. Malondialdehyde (MDA), total antioxidant (TAS) levels, and paraoxanase (PON) and arylesterase enzyme activities were examined as well as Coenzyme Q10, that has an immense role in production of ATP and, in fact, is a potent antioxidant with a lipophillic structure Methods: Research included 160 men and women whose ages differ from 20 to 70 years old and also live in Gaziantep area. Research was done by dividing subjects into 3 age groups: 50 people belonged to 20–30, 75 people to 31–50 and 35 people to 51–70 age group. Groups were constituted from people who are non-obese and who do not have any physical complaints. In taken plasma samples coenzyme Q10, MDA and TAS levels, PON and arylesterase enzyme activities were measured. The measurements were performed as follows: MDA by using thiobarbituric acid method, TAS, PON and arylesterase with spectrophotometric methods, and coenzyme Q10 with HPLC method were measured. Results: A correlation was found between age and coenzyme Q10 in our study (p<0.001). Coenzyme Q10 was found to be increased with aging. Statistically significant and negative linear relationship was found between coenzyme Q10, MDA levels, and PON and arylesterase activities (p<0.01, p<0.001). Conclusion: Despite the aging, the antioxidant system in healthy people works to protect the body from the endogenous or exogenous oxidants. However, levels of antioxidants cannot compansate with the rising oxidant levels in case of an illness. Aging is not a disease itself, but the process of aging is accelerated by a disease.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"6 1","pages":"18-24"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76048514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: In reliable clinical laboratory practice, to use test results for the maximum benefit of patients, the new method in a laboratory should be validated. In this study our purpose is to evaluate performance characteristics of cystatin C immunoturbidimetric method in Roche Cobas Integra 800 analyser, and compare this method with immunonephelometric method in Dade Behring BNII analyser. Methods: Precision, linearity, recovery, interference experiments in Roche Cobas Integra 800 analyser and method comparison experiments were performed for cystatin C. Results: For Roche Cobas Integra 800 instrument, low and high concentrations for withinrun and day to day CV values were 3.97%, 1.32% and 7.24%, 4.16% respectively. In linearity experiment, regression graph equation was found to be y = 0.9817x 0.0149 and r2 = 0.99. In recovery experiment, % recovery was found to be 81. In hemolysis interference experiment, interference was detected for the hemoglobin concetrations above 200 mg/dL, Method comparison experiment was performed by analyzing 100 patients serum in both Dade Behring BNII nephelometry and Roche Cobas Integra 800 analyser. Correlation factor was r2=0.95, Deming regression equation was y = 0,98x + 0,22. Conclusion: It was found for turbidimetric method, CV values were higher, % recovery was lower and hemolysis interference was detected at lower concentrations than what was stated in package insert of cystatin C kits. However, Deming regression results indicated turbidimetric and nephelometric methods were compatible.
{"title":"Methodological evaluation of a turbidimetric method for the analysis of serum cystatin c and comparison with a nephelometric method","authors":"A. Toprak, B. Kinas, A. Uras","doi":"10.5505/TJB.2013.81300","DOIUrl":"https://doi.org/10.5505/TJB.2013.81300","url":null,"abstract":"Objective: In reliable clinical laboratory practice, to use test results for the maximum benefit of patients, the new method in a laboratory should be validated. In this study our purpose is to evaluate performance characteristics of cystatin C immunoturbidimetric method in Roche Cobas Integra 800 analyser, and compare this method with immunonephelometric method in Dade Behring BNII analyser. Methods: Precision, linearity, recovery, interference experiments in Roche Cobas Integra 800 analyser and method comparison experiments were performed for cystatin C. Results: For Roche Cobas Integra 800 instrument, low and high concentrations for withinrun and day to day CV values were 3.97%, 1.32% and 7.24%, 4.16% respectively. In linearity experiment, regression graph equation was found to be y = 0.9817x 0.0149 and r2 = 0.99. In recovery experiment, % recovery was found to be 81. In hemolysis interference experiment, interference was detected for the hemoglobin concetrations above 200 mg/dL, Method comparison experiment was performed by analyzing 100 patients serum in both Dade Behring BNII nephelometry and Roche Cobas Integra 800 analyser. Correlation factor was r2=0.95, Deming regression equation was y = 0,98x + 0,22. Conclusion: It was found for turbidimetric method, CV values were higher, % recovery was lower and hemolysis interference was detected at lower concentrations than what was stated in package insert of cystatin C kits. However, Deming regression results indicated turbidimetric and nephelometric methods were compatible.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"22 1","pages":"239-242"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80490280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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