T. O. Oladosu, A. Awoseemo, A. Olawumi, J. Oladosu
The objective of this study was to assess the practice of injection safety among nurses in Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife, Osun State, Nigeria. A cross sectional descriptive design was adopted for the study. A self-administered questionnaire was used to obtain information. A total of 271 nurses were selected from various wards of the hospital using stratified sampling technique. The data were collected, assembled and analyzed by using SPSS version 20.0 at p˂0.05 significance level. Findings showed that 60% of the respondents demonstrated good practice of injection safety. However, the major barriers to the practice of injection safety were availability of good disposable injection equipment (p= 0.040), non-display of injection safety guidelines (p= 0.010) and nurses’ lack of adequate knowledge (p= 0.003). Training and retraining of nurses should be embarked upon in order to improve nurses’ knowledge of injection safety and enhance good practice. The institution should demonstrate willingness to safety injection practices by providing adequate resources for its implementation. Keywords: injection safety, barriers to injection practice, nurses, tertiary hospital, Nigeria
{"title":"Assessment of Practice of Injection Safety among Nurses in a Tertiary Hospital in Osun State, Nigeria","authors":"T. O. Oladosu, A. Awoseemo, A. Olawumi, J. Oladosu","doi":"10.47430/ujmr.2162.001","DOIUrl":"https://doi.org/10.47430/ujmr.2162.001","url":null,"abstract":"The objective of this study was to assess the practice of injection safety among nurses in Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife, Osun State, Nigeria. A cross sectional descriptive design was adopted for the study. A self-administered questionnaire was used to obtain information. A total of 271 nurses were selected from various wards of the hospital using stratified sampling technique. The data were collected, assembled and analyzed by using SPSS version 20.0 at p˂0.05 significance level. Findings showed that 60% of the respondents demonstrated good practice of injection safety. However, the major barriers to the practice of injection safety were availability of good disposable injection equipment (p= 0.040), non-display of injection safety guidelines (p= 0.010) and nurses’ lack of adequate knowledge (p= 0.003). Training and retraining of nurses should be embarked upon in order to improve nurses’ knowledge of injection safety and enhance good practice. The institution should demonstrate willingness to safety injection practices by providing adequate resources for its implementation. Keywords: injection safety, barriers to injection practice, nurses, tertiary hospital, Nigeria","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85325341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hepatitis B virus infection is a global public health issue. It can result in hepatocellular carcinoma (HCC), and liver cirrhosis when not properly managed. The discovery of Hepatitis B vaccine and its incorporation into immunization programmes has brought a drastic decline in the incidence of both chronic and acute hepatitis B. However, this achievement is being confronted by cases of vaccine sub-optimal immune response, non-responsiveness and breakthrough infection which may be associated with the host’s genetic predisposition such as; ABO/Rhesus blood groups and hemoglobin genotype. The study aimed at investigating hepatitis B vaccines responsiveness (immunogenicity) and its association with the hosts’ ABO and Rhesus blood group among vaccinated subjects in Bauchi State Nigeria. This was a cross-sectional investigation comprising of 352 subjects of both sexes from age 1 year to 60 years. Out of the 352 subjects, 196 were vaccinated while 156 were unvaccinated. 5mL of blood samples were collected and analyzed for determination of both ABO and Rhesus blood group while the plasma part of the samples was tested for anti-HBs antibodies by enzyme linked immunosorbent assay (ELISA). The results revealed that 96(49.0%) of the vaccinated subjects had anti-HBs level ≥ 10 IU/L, 65(33.2%) had ≤ 10 IU/L and 35(17.9%) were non-responders while 26(16.7%) of the unvaccinated subjects had anti-HBs level ≥ 10 IU/L (P = 0.003). On the relationship between the ABO/Rhesus group and vaccine immunogenicity, blood group AB had 100% sub-optimal response while group B had 34.2% suboptimal response and 21% non-responders. Blood group A and O had the highest ≥ 10 IU/L antiHBs protective level of 52.8% and 50.0% respectively. However, there was no significant association between HBV vaccine responsiveness and hosts’ ABO/Rhesus. The protective rate against HBV infection was moderate. Nevertheless, some blood types had higher responses than others. Therefore, a considerable proportion of vaccinated persons should be considered for either booster doses or revaccination. Keywords: ABO; Hepatitis B Virus; Rhesus blood group; Vaccine Immunogenicity.
{"title":"Evaluation of Hepatitis B vaccine Immunogenicity in Relation to ABO and Rhesus Blood Group in vaccinated subjects in Bauchi State, Nigeria","authors":"K. M. Thomas, H. Zakari, P. Lar, T. Vem","doi":"10.47430/ujmr.2162.020","DOIUrl":"https://doi.org/10.47430/ujmr.2162.020","url":null,"abstract":"Hepatitis B virus infection is a global public health issue. It can result in hepatocellular carcinoma (HCC), and liver cirrhosis when not properly managed. The discovery of Hepatitis B vaccine and its incorporation into immunization programmes has brought a drastic decline in the incidence of both chronic and acute hepatitis B. However, this achievement is being confronted by cases of vaccine sub-optimal immune response, non-responsiveness and breakthrough infection which may be associated with the host’s genetic predisposition such as; ABO/Rhesus blood groups and hemoglobin genotype. The study aimed at investigating hepatitis B vaccines responsiveness (immunogenicity) and its association with the hosts’ ABO and Rhesus blood group among vaccinated subjects in Bauchi State Nigeria. This was a cross-sectional investigation comprising of 352 subjects of both sexes from age 1 year to 60 years. Out of the 352 subjects, 196 were vaccinated while 156 were unvaccinated. 5mL of blood samples were collected and analyzed for determination of both ABO and Rhesus blood group while the plasma part of the samples was tested for anti-HBs antibodies by enzyme linked immunosorbent assay (ELISA). The results revealed that 96(49.0%) of the vaccinated subjects had anti-HBs level ≥ 10 IU/L, 65(33.2%) had ≤ 10 IU/L and 35(17.9%) were non-responders while 26(16.7%) of the unvaccinated subjects had anti-HBs level ≥ 10 IU/L (P = 0.003). On the relationship between the ABO/Rhesus group and vaccine immunogenicity, blood group AB had 100% sub-optimal response while group B had 34.2% suboptimal response and 21% non-responders. Blood group A and O had the highest ≥ 10 IU/L antiHBs protective level of 52.8% and 50.0% respectively. However, there was no significant association between HBV vaccine responsiveness and hosts’ ABO/Rhesus. The protective rate against HBV infection was moderate. Nevertheless, some blood types had higher responses than others. Therefore, a considerable proportion of vaccinated persons should be considered for either booster doses or revaccination. Keywords: ABO; Hepatitis B Virus; Rhesus blood group; Vaccine Immunogenicity.","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"117 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88241210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Solomon Matthias Gamde, P. J. Tongvwam, K. Hauwa, A. Ganau, J. Abdullahi, D. S. Gamde, T. Choji
Urinary schistosomiasis is a severe threat to global health with uncountable morbidities in Africa including Nigeria where control interventions focused on children in public and private schools neglecting Almajiri children. This undermined control interventions as those infected contaminate the environments with infective stages of the parasite. The objective of the study was to identify the prevalence of urinary schistosomiasis amongst Almajiri children in Silame, Sokoto State, North-western Nigeria. This was a cross-sectional descriptive study, socio-demographic data was collected in April 2020 on 206 consented Almajiri children in Silame and their urine samples were examined using the sedimentation method. The study showed a prevalence of 35.4% among the Almajiri children in Silame, Sokoto State, North-western Nigeria. The highest prevalence was found among children within the age range 16-20 years (63.6%) while the lowest prevalence was among those in the age range 6-10 years (24.4%). There was a statistically significant difference in the occurrence of urinary schistosomiasis between the age groups (χ 2 = 11.637a , df =3, p=0.002). Urinary schistosomiasis was prevalent among Almajiri children in the study area and parasite infection was associated with the participant's socio-demographic factors such as age, level of education, and water contact activities. Hence, the National Schistosomiasis Control Programs should incorporate the Almajiri children in the control interventions Keywords: Schistosoma hematobium infection; Makarantarallo;Almajiri;Silame
{"title":"Prevalence of Urinary Schistosomiasis among Almajiri Children in Silame, Sokoto State, North-western Nigeria","authors":"Solomon Matthias Gamde, P. J. Tongvwam, K. Hauwa, A. Ganau, J. Abdullahi, D. S. Gamde, T. Choji","doi":"10.47430/ujmr.2161.013","DOIUrl":"https://doi.org/10.47430/ujmr.2161.013","url":null,"abstract":"Urinary schistosomiasis is a severe threat to global health with uncountable morbidities in Africa including Nigeria where control interventions focused on children in public and private schools neglecting Almajiri children. This undermined control interventions as those infected contaminate the environments with infective stages of the parasite. The objective of the study was to identify the prevalence of urinary schistosomiasis amongst Almajiri children in Silame, Sokoto State, North-western Nigeria. This was a cross-sectional descriptive study, socio-demographic data was collected in April 2020 on 206 consented Almajiri children in Silame and their urine samples were examined using the sedimentation method. The study showed a prevalence of 35.4% among the Almajiri children in Silame, Sokoto State, North-western Nigeria. The highest prevalence was found among children within the age range 16-20 years (63.6%) while the lowest prevalence was among those in the age range 6-10 years (24.4%). There was a statistically significant difference in the occurrence of urinary schistosomiasis between the age groups (χ 2 = 11.637a , df =3, p=0.002). Urinary schistosomiasis was prevalent among Almajiri children in the study area and parasite infection was associated with the participant's socio-demographic factors such as age, level of education, and water contact activities. Hence, the National Schistosomiasis Control Programs should incorporate the Almajiri children in the control interventions Keywords: Schistosoma hematobium infection; Makarantarallo;Almajiri;Silame","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"262 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75679336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Bello, J. Ameh, D. Machido, A. I. Mohammed-Dabo
Laccases are oxidases with broad substrate specificity and ability to oxidize various phenolic and non-phenolic compounds. This study was carried out to isolate and characterizes laccase producing fungi from environment samples. Soil and decaying wood samples were collected from different locations within Ahmadu Bello University, Zaria Main campus. Suspensions of the samples (1 g in 10 mL sterile distilled water) were serially diluted, inoculated onto Potato Dextrose Agar (PDA) containing 0.01% Chloramphenicol and incubated for 7 days at 30oC.The fungal isolates were characterized macroscopically and microscopically with the aid of an atlas. The identified fungal isolates were screened for laccase production by inoculating onto PDA containing 0.02% Guaiacol, 1mM ABTS (2 2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) and 0.5% Tannic acid as indicator compounds and incubated at 250C for 7 days. The laccase producing isolates were confirmed molecularly by ITS rDNA sequence analysis using the FASTA algorithm with the Fungus database from the European Bioinformatics Institute (EBI).A total of 25 fungal species (11 from soil and 14 from decaying wood samples) were isolated. Two isolates from the soil origin identified as Curvularia lunata SSI7 (Accession No. QIE06317.1) and Fusarium clade VII SSI3 (Accession No. GQ505677) were found to produce laccase where Curvularia lunata SSI7 was able to oxidize all the indicator compounds used for the screening. Fusarium clade VII SSI3 was able to oxidize only 0.5% Tannic acid. Laccase producing Curvularia lunata and Fusarium clade VII were isolated from soil samples collected from ABU Zaria Main Campus. Keywords: laccase, fungi, soil, decaying wood
{"title":"Screening and Identification of Laccase Producing Fungi from Environmental Samples","authors":"A. Bello, J. Ameh, D. Machido, A. I. Mohammed-Dabo","doi":"10.47430/ujmr.2161.012","DOIUrl":"https://doi.org/10.47430/ujmr.2161.012","url":null,"abstract":"Laccases are oxidases with broad substrate specificity and ability to oxidize various phenolic and non-phenolic compounds. This study was carried out to isolate and characterizes laccase producing fungi from environment samples. Soil and decaying wood samples were collected from different locations within Ahmadu Bello University, Zaria Main campus. Suspensions of the samples (1 g in 10 mL sterile distilled water) were serially diluted, inoculated onto Potato Dextrose Agar (PDA) containing 0.01% Chloramphenicol and incubated for 7 days at 30oC.The fungal isolates were characterized macroscopically and microscopically with the aid of an atlas. The identified fungal isolates were screened for laccase production by inoculating onto PDA containing 0.02% Guaiacol, 1mM ABTS (2 2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) and 0.5% Tannic acid as indicator compounds and incubated at 250C for 7 days. The laccase producing isolates were confirmed molecularly by ITS rDNA sequence analysis using the FASTA algorithm with the Fungus database from the European Bioinformatics Institute (EBI).A total of 25 fungal species (11 from soil and 14 from decaying wood samples) were isolated. Two isolates from the soil origin identified as Curvularia lunata SSI7 (Accession No. QIE06317.1) and Fusarium clade VII SSI3 (Accession No. GQ505677) were found to produce laccase where Curvularia lunata SSI7 was able to oxidize all the indicator compounds used for the screening. Fusarium clade VII SSI3 was able to oxidize only 0.5% Tannic acid. Laccase producing Curvularia lunata and Fusarium clade VII were isolated from soil samples collected from ABU Zaria Main Campus. Keywords: laccase, fungi, soil, decaying wood","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77862500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Techno-industrial advancements the world over had led to the generation of hazardous environmental pollutants. Microbial bioremediation offers the best alternative for the removal of these pollutants. The most recent advancements in microbial bioremediation were catalyzed by the advent of various tools that enable the study microbes at levels of sophisticated detail, including genome analysis tools (genomics), protocols for analyzing expressed proteins and enzymes or proteomes (proteomics), techniques of analyzing ribonucleic acids (RNAs) transcriptomes (transcriptomics), and tools for analyzing metabolic end products/metabolomes (metabolomics). The twenty first century is witnessing an outpour of developments in the application of omics approaches in effective microbial bioremediation, thus, this paper attempts to review some of the most significant insights gained from relatively recent studies over a period of two decades (2000-2020) in the applications of multi-OMICS in microbial bioremediation, including trends and cutting-edge researches. We aim to highlight, particularly, the challenges that need to be overcome before OMICs approaches are successfully enshrined in microbial bioremediation, especially in developing countries. The strategies for overcoming such challenges, and the prospects achieved were also outlined. In the coming years, we envision further researches involving the application of multi-OMICs approach in microbial bioremediation potentially revolutionizing this field, opening up research avenues, and leading to improvements in bioremediation of polluted environment. Keywords: Biodegradation, Bioremediation; Genomics; Multi-OMICs, OMICs techniques.
{"title":"Effective microbial bioremediation via the multi-omics approach: An overview of trends, problems and prospects","authors":"Y. R. Yunusa, Zubairu Darma Umar","doi":"10.47430/ujmr.2161.017","DOIUrl":"https://doi.org/10.47430/ujmr.2161.017","url":null,"abstract":"Techno-industrial advancements the world over had led to the generation of hazardous environmental pollutants. Microbial bioremediation offers the best alternative for the removal of these pollutants. The most recent advancements in microbial bioremediation were catalyzed by the advent of various tools that enable the study microbes at levels of sophisticated detail, including genome analysis tools (genomics), protocols for analyzing expressed proteins and enzymes or proteomes (proteomics), techniques of analyzing ribonucleic acids (RNAs) transcriptomes (transcriptomics), and tools for analyzing metabolic end products/metabolomes (metabolomics). The twenty first century is witnessing an outpour of developments in the application of omics approaches in effective microbial bioremediation, thus, this paper attempts to review some of the most significant insights gained from relatively recent studies over a period of two decades (2000-2020) in the applications of multi-OMICS in microbial bioremediation, including trends and cutting-edge researches. We aim to highlight, particularly, the challenges that need to be overcome before OMICs approaches are successfully enshrined in microbial bioremediation, especially in developing countries. The strategies for overcoming such challenges, and the prospects achieved were also outlined. In the coming years, we envision further researches involving the application of multi-OMICs approach in microbial bioremediation potentially revolutionizing this field, opening up research avenues, and leading to improvements in bioremediation of polluted environment. Keywords: Biodegradation, Bioremediation; Genomics; Multi-OMICs, OMICs techniques.","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76610537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Aliyu, M. S. Shamsiyya, Z. Lambu, A. Sabo, Habu Haruna
Vaginitis is usually caused by a change in the normal balance of vaginal microbes, an infection or reduced oestrogen levels after menopause leading to variety of infections by bacteria, fungi and viruses. The study aimed to determine the prevalence of vaginitis and its associated risk factors among women attending antenatal care center of Wudil general hospital, Kano Nigeria. A total of 352 High Vaginal Swab and serum samples were collected from women attending antenatal care centre of Wudil general hospital. The samples were processed and screened for the presence of Candida albicans, Trichomonas vaginalis and Treponema pallidum through culture and microscopy and serum venereal disease research laboratory (VDRL) test. The risk factors were assessed using a designed questionnaire and analysed using Multivariate logistic modelling. The prevalence of C. albicans, T. vaginalis and Treponema pallidum were found to be 32.38%, 18.46 %, and 4.83%, respectively(p≤0.05). The result showed that the risk factors that are significantly associated with the prevalence of vaginitis were active sex age (26 – 30) years, educational status and number of participants husband’s wives. The study identifies that C. albicans is the major cause of vaginitis, and is insignificantly associated with active sex age educational status and number of participants husband’s wives. Keywords: Candida albicans, Trichomonas vaginalis, Treponema pallidum, Pregnant Women
{"title":"Prevalence of Vaginitis among Women Attending Antenatal Care Centre of Wudil General Hospital, Kano State Nigeria","authors":"A. Aliyu, M. S. Shamsiyya, Z. Lambu, A. Sabo, Habu Haruna","doi":"10.47430/ujmr.2161.003","DOIUrl":"https://doi.org/10.47430/ujmr.2161.003","url":null,"abstract":"Vaginitis is usually caused by a change in the normal balance of vaginal microbes, an infection or reduced oestrogen levels after menopause leading to variety of infections by bacteria, fungi and viruses. The study aimed to determine the prevalence of vaginitis and its associated risk factors among women attending antenatal care center of Wudil general hospital, Kano Nigeria. A total of 352 High Vaginal Swab and serum samples were collected from women attending antenatal care centre of Wudil general hospital. The samples were processed and screened for the presence of Candida albicans, Trichomonas vaginalis and Treponema pallidum through culture and microscopy and serum venereal disease research laboratory (VDRL) test. The risk factors were assessed using a designed questionnaire and analysed using Multivariate logistic modelling. The prevalence of C. albicans, T. vaginalis and Treponema pallidum were found to be 32.38%, 18.46 %, and 4.83%, respectively(p≤0.05). The result showed that the risk factors that are significantly associated with the prevalence of vaginitis were active sex age (26 – 30) years, educational status and number of participants husband’s wives. The study identifies that C. albicans is the major cause of vaginitis, and is insignificantly associated with active sex age educational status and number of participants husband’s wives. Keywords: Candida albicans, Trichomonas vaginalis, Treponema pallidum, Pregnant Women","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90151838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The telecommunication mast associated-radiation is one of the primary factors influencing the way in which microorganisms interact with ecosystem. This study aims to assess the physicochemical and non-symbiotic nitrogen fixing bacteria (NNFB) from soil samples within the vicinity of telecommunication mast located at Karfi town Kura local government Kano state. Soil samples A, B, and C were collected within the vicinity of the mast at an interval of 10 meters, 20 meters and 30 meters from the mast respectively and control sample (D) was collected from location *(outside the vicinity of the mast)*. Physicochemical parameters of the soil samples were analyzed, isolation and identification of non-symbiotic nitrogen fixing bacteria were carried out using standard procedures. Samples B showed higher values of pH (8.02), phosphorus (23.95mg/kg), organic carbon (1.45%), nitrogen (0.28%) and organic matter content (2.50%) while control sample (D) showed lower values with 6.24, 2.77mg/kg, 0.41%, 0.07% and 0.71% of pH, phosphorus, organic carbon, nitrogen and organic matter content respectively. However, the moisture content(0.21%) of control sample is higher than that of sample A and B with 0.12% and 0.11% respectively The mean count of NNFB of the soil samples were 3.20 ± 0.06, 1.80± 0.12, 1.40±0.23, 1.20±0.20 for sample B, C, A and D respectively. Total of 14 isolates of the species Azomonas agilis 1(7.14%), Azotomonas insolita 1(7.14%), Bacillus megaterium 2(14.28 %), Bacillus azotoformans 1(7.14%), Bacillus mycoides 3(21.42%), Enterobacter cloacae 3(21.42%), and Klebsiella pneumonia 3(21.42%) were obtained. This indicates that the electromagnetic radiation from the mast has no effect on soil physicochemical parameters as well as non symbiotic nitrogen fixing bacteria proliferation. Key words: Non symbiotic Bacteria, telecommunication mast
{"title":"Physicochemical and Characterization of Nitrogen Fixing Bacteria from Soil Samples Within the Vicinity of Telecommunication Mast (Site No: 000148) Located at Karfi Town Kura Local Government, Kano State","authors":"I. Lawal, I. Yusuf","doi":"10.47430/ujmr.2161.010","DOIUrl":"https://doi.org/10.47430/ujmr.2161.010","url":null,"abstract":"The telecommunication mast associated-radiation is one of the primary factors influencing the way in which microorganisms interact with ecosystem. This study aims to assess the physicochemical and non-symbiotic nitrogen fixing bacteria (NNFB) from soil samples within the vicinity of telecommunication mast located at Karfi town Kura local government Kano state. Soil samples A, B, and C were collected within the vicinity of the mast at an interval of 10 meters, 20 meters and 30 meters from the mast respectively and control sample (D) was collected from location *(outside the vicinity of the mast)*. Physicochemical parameters of the soil samples were analyzed, isolation and identification of non-symbiotic nitrogen fixing bacteria were carried out using standard procedures. Samples B showed higher values of pH (8.02), phosphorus (23.95mg/kg), organic carbon (1.45%), nitrogen (0.28%) and organic matter content (2.50%) while control sample (D) showed lower values with 6.24, 2.77mg/kg, 0.41%, 0.07% and 0.71% of pH, phosphorus, organic carbon, nitrogen and organic matter content respectively. However, the moisture content(0.21%) of control sample is higher than that of sample A and B with 0.12% and 0.11% respectively The mean count of NNFB of the soil samples were 3.20 ± 0.06, 1.80± 0.12, 1.40±0.23, 1.20±0.20 for sample B, C, A and D respectively. Total of 14 isolates of the species Azomonas agilis 1(7.14%), Azotomonas insolita 1(7.14%), Bacillus megaterium 2(14.28 %), Bacillus azotoformans 1(7.14%), Bacillus mycoides 3(21.42%), Enterobacter cloacae 3(21.42%), and Klebsiella pneumonia 3(21.42%) were obtained. This indicates that the electromagnetic radiation from the mast has no effect on soil physicochemical parameters as well as non symbiotic nitrogen fixing bacteria proliferation. Key words: Non symbiotic Bacteria, telecommunication mast","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78689693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Lambu, U. Shamsuddeen, S. Yahaya, A. Aliyu, A. Shehu, A. Karfi, J. Isyaku, T. Mahmud, Y. Mohammed
Tannery waste is made from nutrients and organic compounds that enhance soil fertility thereby improving plants and microbial growths. This study was designed to investigate the application of tannery wastes to form compost material in a mixture with cow-dung and rice brand. The treatments were prepared and tested for a period of sixty (60) days. The compost was characterized by electric conductivity (EC) of 10.11µs/cm, pH of 7.62 and Carbon-Nitrogen (C:N) ratio of 28.8. Total concentrations of Chromium, Lead, Cadmium, Copper, Zinc and Iron in mg/kg were 57.2, 0.92, 12.50, 60.50, 13.60 and 1101.00 respectively. However, total bacterial counts decreased from 8.2 x108 cfu/g to 1.8 x106 cfu/g after the 60days. The compost characteristics indicate about 80% germination index which may suggest the removal of most of the phototoxic compounds. Key words: Composting, Tannery wastes, bio-fertilizer
{"title":"Production of Bio-Fertilizer through Composting of Tannery Wastes with CowDung and Rice Bran","authors":"Z. Lambu, U. Shamsuddeen, S. Yahaya, A. Aliyu, A. Shehu, A. Karfi, J. Isyaku, T. Mahmud, Y. Mohammed","doi":"10.47430/ujmr.2161.001","DOIUrl":"https://doi.org/10.47430/ujmr.2161.001","url":null,"abstract":"Tannery waste is made from nutrients and organic compounds that enhance soil fertility thereby improving plants and microbial growths. This study was designed to investigate the application of tannery wastes to form compost material in a mixture with cow-dung and rice brand. The treatments were prepared and tested for a period of sixty (60) days. The compost was characterized by electric conductivity (EC) of 10.11µs/cm, pH of 7.62 and Carbon-Nitrogen (C:N) ratio of 28.8. Total concentrations of Chromium, Lead, Cadmium, Copper, Zinc and Iron in mg/kg were 57.2, 0.92, 12.50, 60.50, 13.60 and 1101.00 respectively. However, total bacterial counts decreased from 8.2 x108 cfu/g to 1.8 x106 cfu/g after the 60days. The compost characteristics indicate about 80% germination index which may suggest the removal of most of the phototoxic compounds. Key words: Composting, Tannery wastes, bio-fertilizer","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85107260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mobile phones are increasingly becoming one of the indispensable accessories of professional and social daily life, although constant handling and usage in various environmental conditions makes it fomite. Thus, antibacterial activity of Psidium guajava leaf extract was evaluated against bacteria isolated from mobile phones within Umar Musa Yar’adua University, Katsina community. A total of sixty (60) mobile phones were randomly swabbed, twenty (20) each, from hawkers, students, and staff of the University community. These were used as candidates for isolation of bacterial contaminants using standard protocols. Variable concentrations (500mg/ml, 300mg/ml and 100mg/ml) of aqueous extract of the Psidium guajava were prepared and tested against mobile phones bacterial isolates using agar well diffusion method. Preliminary phytochemical screening revealed the presence of flavonoids, cardiac glycosides, saponins, tannins and terpenoids. Staphylococcus aureus (39.6%), Escherichia coli (29.7%), Klebsiella sp. (18.8%), Proteus sp. (8.3%) and Pseudomonas aeruginosa (4.2%) were the bacterial contaminants isolated and identified from the mobile phones. Aqueous leaf extract of the plant displayed promising antibacterial activity at 500mg/ml against all the isolates, with average zones of inhibition of 25.0 mm for S. aureus, Proteus sp., P. aeroginosa and 24mm, 6.0mm for E. coli and Klebsiella sp. respectively. The use of Psidium guajava leaf extract as candidate for production of antibacterial agent which can be used to disinfect mobile handsets is suggested. Keywords: Mobile phones, contaminants, Antibacterial activity, Psidium guajava, antibacterial hand wash
手机越来越成为职业和社会日常生活中不可缺少的配件之一，尽管在各种环境条件下的不断处理和使用使其变得有害。因此，本研究评价了番石榴叶提取物对Umar Musa Yar 'adua University, Katsina社区手机分离细菌的抑菌活性。从小贩、学生和大学社区工作人员中随机抽取了60(60)部手机，各20(20)部手机。这些被用作使用标准方案分离细菌污染物的候选物。制备不同浓度(500mg/ml、300mg/ml、100mg/ml)瓜爪哇番石榴水提液，采用琼脂孔扩散法对手机分离菌进行抑菌试验。初步的植物化学筛选发现黄酮类化合物、心苷类、皂苷类、单宁类和萜类化合物。从手机中分离鉴定出的细菌污染物为金黄色葡萄球菌(39.6%)、大肠杆菌(29.7%)、克雷伯氏菌(18.8%)、变形杆菌(8.3%)和铜绿假单胞菌(4.2%)。该植物叶片水提液在500mg/ml浓度下对所有菌株均表现出良好的抑菌活性，对金黄色葡萄球菌、变形杆菌、铜绿假单胞菌的平均抑菌面积为25.0 mm，对大肠杆菌和克雷伯氏菌的平均抑菌面积分别为24mm、6.0mm。建议将番石榴叶提取物作为手机消毒用抗菌剂的候选原料。关键词:手机，污染物，抗菌活性，瓜石榴金，抗菌洗手液
{"title":"Determination of Antibacterial Activity of Psidium guajava Leaf Extract against Bacteria Isolated from Mobile Phones of Umaru Musa Yar’adua University, Katsina Community","authors":"B. Abdulkadir, A. Ado, A. Alli","doi":"10.47430/ujmr.2161.032","DOIUrl":"https://doi.org/10.47430/ujmr.2161.032","url":null,"abstract":"Mobile phones are increasingly becoming one of the indispensable accessories of professional and social daily life, although constant handling and usage in various environmental conditions makes it fomite. Thus, antibacterial activity of Psidium guajava leaf extract was evaluated against bacteria isolated from mobile phones within Umar Musa Yar’adua University, Katsina community. A total of sixty (60) mobile phones were randomly swabbed, twenty (20) each, from hawkers, students, and staff of the University community. These were used as candidates for isolation of bacterial contaminants using standard protocols. Variable concentrations (500mg/ml, 300mg/ml and 100mg/ml) of aqueous extract of the Psidium guajava were prepared and tested against mobile phones bacterial isolates using agar well diffusion method. Preliminary phytochemical screening revealed the presence of flavonoids, cardiac glycosides, saponins, tannins and terpenoids. Staphylococcus aureus (39.6%), Escherichia coli (29.7%), Klebsiella sp. (18.8%), Proteus sp. (8.3%) and Pseudomonas aeruginosa (4.2%) were the bacterial contaminants isolated and identified from the mobile phones. Aqueous leaf extract of the plant displayed promising antibacterial activity at 500mg/ml against all the isolates, with average zones of inhibition of 25.0 mm for S. aureus, Proteus sp., P. aeroginosa and 24mm, 6.0mm for E. coli and Klebsiella sp. respectively. The use of Psidium guajava leaf extract as candidate for production of antibacterial agent which can be used to disinfect mobile handsets is suggested. Keywords: Mobile phones, contaminants, Antibacterial activity, Psidium guajava, antibacterial hand wash","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"94 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87507952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study was undertaken to evaluate the bacteriology and antibiogram of isolates from diabetic patients with chronic foot ulcers in Nigeria. A total of 150 pus samples were collected and processed according to standard aerobic and anaerobic microbiological methods. Antibiogram was done using Kirby-Bauer method. Biofilm tests, ESBL & AmpC production was conducted using Congo red agar, Double disc synergy test and Cefoxitin disc test respectively. Total number of isolates obtained was 210. The Plasmid profiles of some of the Multi-Drug Resistance (MDR) isolates were carried out using the alkaline lysis method for plasmid extraction and electrophoresis on agarose gel with standard markers. The most frequently isolated aerobic organism in the study was Escherichia coli (32.1%) while the least occurring was Enterobacter spp (1.57%). For the anaerobes, Peptostreptococcus spp (40%) was the highest isolated bacterium. Percentage of Extended Spectrum -lactamase ( ESBL) producers among E. coli isolates was 44%. Percentages of biofilm formation potential among the isolates were: E. coli (36.8%), S. aureus (23.1%) and Proteus vulgaris (4.2%). Escherichia coli and S. aureus showed considerable levels of resistance to some common antibiotics. No methicilin resistant S. aureus was encountered. AmpC producers encountered were Klebsiella pneumonia (10%) and E. coli (8.1%). Post-curring antibiogram tests revealed that nine isolates carried plasmids, suggesting that the mode of resistance may be plasmid mediated. Keywords: Diabetic ulcers, Bacteria, Antibiogram, Plasmid profile
{"title":"Antibiotic Sensitivity Pattern and Plasmid Profile of Bacteria Isolated from Diabetic Ulcers in Mbano Metropolis, Imo State, Southeastern Nigeria","authors":"E. Nwankwo, E. Nwagbara, K. Onusiriuka","doi":"10.47430/ujmr.2161.005","DOIUrl":"https://doi.org/10.47430/ujmr.2161.005","url":null,"abstract":"The study was undertaken to evaluate the bacteriology and antibiogram of isolates from diabetic patients with chronic foot ulcers in Nigeria. A total of 150 pus samples were collected and processed according to standard aerobic and anaerobic microbiological methods. Antibiogram was done using Kirby-Bauer method. Biofilm tests, ESBL & AmpC production was conducted using Congo red agar, Double disc synergy test and Cefoxitin disc test respectively. Total number of isolates obtained was 210. The Plasmid profiles of some of the Multi-Drug Resistance (MDR) isolates were carried out using the alkaline lysis method for plasmid extraction and electrophoresis on agarose gel with standard markers. The most frequently isolated aerobic organism in the study was Escherichia coli (32.1%) while the least occurring was Enterobacter spp (1.57%). For the anaerobes, Peptostreptococcus spp (40%) was the highest isolated bacterium. Percentage of Extended Spectrum -lactamase ( ESBL) producers among E. coli isolates was 44%. Percentages of biofilm formation potential among the isolates were: E. coli (36.8%), S. aureus (23.1%) and Proteus vulgaris (4.2%). Escherichia coli and S. aureus showed considerable levels of resistance to some common antibiotics. No methicilin resistant S. aureus was encountered. AmpC producers encountered were Klebsiella pneumonia (10%) and E. coli (8.1%). Post-curring antibiogram tests revealed that nine isolates carried plasmids, suggesting that the mode of resistance may be plasmid mediated. Keywords: Diabetic ulcers, Bacteria, Antibiogram, Plasmid profile","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83009777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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