The increasing discharge of dyes into the environment and their consequential ecological effects has necessitated the need for the ecofriendly decolourization methods. Laccases became enzymes of research interest due to their broad substrates specificities. The aim of the study was to purify, characterize and determine the decolourization ability of Curvularia lunata SS17 produced laccase. Laccase was produced using maize cob as substrate under optimized culture conditions and purified using Gel Filtration Chromatography. Biuret method was then used to estimate the protein contents of the crude and partially purified laccase. Specific activities, purification fold and yield (%) of the crude and purified laccase enzyme were also estimated. Decolourization potentials of the crude and partially purified enzyme were evaluated using four dyes namely: Congo red, Methylene blue, Bromocresol green and direct yellow. Elution profile of partially purified laccase revealed that fraction 5 had the highest laccase activity (3.654 U/mL). Optimum conditions for enzyme activity were estimated to be 35oC and pH 6. Enzyme activity of the partially purified laccase (3.654 U/mL) was observed to be higher than that of the crude laccase (1.635 U/mL). Also, the partially purified laccase had higher specific activity (1.87 U/mg) compared to that of the crude laccase (0.41 U/mg). Higher percentage dye decolourization potential was observed using the partially purified laccase compared to the crude laccase. Increase in percentage decolourization of the dyes by the partially purified laccase as well as crude laccase was observed as incubation time proceeds.
{"title":"Evaluation of Dye Decolourization ability of Laccase Produced Curvularia lunata SS17","authors":"Bello, A., Hussaini, I. M.","doi":"10.47430/ujmr.2272.001","DOIUrl":"https://doi.org/10.47430/ujmr.2272.001","url":null,"abstract":"The increasing discharge of dyes into the environment and their consequential ecological effects has necessitated the need for the ecofriendly decolourization methods. Laccases became enzymes of research interest due to their broad substrates specificities. The aim of the study was to purify, characterize and determine the decolourization ability of Curvularia lunata SS17 produced laccase. Laccase was produced using maize cob as substrate under optimized culture conditions and purified using Gel Filtration Chromatography. Biuret method was then used to estimate the protein contents of the crude and partially purified laccase. Specific activities, purification fold and yield (%) of the crude and purified laccase enzyme were also estimated. Decolourization potentials of the crude and partially purified enzyme were evaluated using four dyes namely: Congo red, Methylene blue, Bromocresol green and direct yellow. Elution profile of partially purified laccase revealed that fraction 5 had the highest laccase activity (3.654 U/mL). Optimum conditions for enzyme activity were estimated to be 35oC and pH 6. Enzyme activity of the partially purified laccase (3.654 U/mL) was observed to be higher than that of the crude laccase (1.635 U/mL). Also, the partially purified laccase had higher specific activity (1.87 U/mg) compared to that of the crude laccase (0.41 U/mg). Higher percentage dye decolourization potential was observed using the partially purified laccase compared to the crude laccase. Increase in percentage decolourization of the dyes by the partially purified laccase as well as crude laccase was observed as incubation time proceeds.","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74947052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Contamination of food and feed by aflatoxins has become a worldwide cause of public health concern due to its significant impact on human health and crop market value. The present study aimed to assess the aflatoxin contents of maize and groundnut from stores and warehouses in the Giwa community, Kaduna State, Nigeria. A total of ninety (90) grain samples of maize and groundnut were collected between October to December 2020 and analyzed for total aflatoxins using Enzyme-Linked Immuno Sorbent Assay (ELISA). Eighty-four 84(93.3%) of the samples had detectable aflatoxin levels (0.2-9.8ppb), while six 6(6.7%) had none. There was no significant difference (P>0.05) in the mean total aflatoxin content of the grains from stores, warehouses and household foodstuff samples. This could be attributed to agricultural practices and low temperature and humidity storage conditions, which were the same for all the stored grains. Overall, aflatoxin concentrations of the grain samples were within the acceptable limit (10ppb for maize and 15ppb for groundnut) for food safety set by NAFDAC. However, there could be further contamination during storage, particularly when temperature rises and humidity increases. Hence, improved storage conditions and monitoring of grain before sale are recommended to avoid contamination during storage and to ensure a healthy and safe food supply along the trade chain and to the consumers.
{"title":"Assessment of Aflatoxin Contamination in Maize and Groundnuts during Storage in Giwa Community, Kaduna State Nigeria","authors":"Abdurrazaq, M., Tijjani, M.B., Atta, H.I.","doi":"10.47430/ujmr.2272.004","DOIUrl":"https://doi.org/10.47430/ujmr.2272.004","url":null,"abstract":"Contamination of food and feed by aflatoxins has become a worldwide cause of public health concern due to its significant impact on human health and crop market value. The present study aimed to assess the aflatoxin contents of maize and groundnut from stores and warehouses in the Giwa community, Kaduna State, Nigeria. A total of ninety (90) grain samples of maize and groundnut were collected between October to December 2020 and analyzed for total aflatoxins using Enzyme-Linked Immuno Sorbent Assay (ELISA). Eighty-four 84(93.3%) of the samples had detectable aflatoxin levels (0.2-9.8ppb), while six 6(6.7%) had none. There was no significant difference (P>0.05) in the mean total aflatoxin content of the grains from stores, warehouses and household foodstuff samples. This could be attributed to agricultural practices and low temperature and humidity storage conditions, which were the same for all the stored grains. Overall, aflatoxin concentrations of the grain samples were within the acceptable limit (10ppb for maize and 15ppb for groundnut) for food safety set by NAFDAC. However, there could be further contamination during storage, particularly when temperature rises and humidity increases. Hence, improved storage conditions and monitoring of grain before sale are recommended to avoid contamination during storage and to ensure a healthy and safe food supply along the trade chain and to the consumers.","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"47 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79455464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Makolo, Daniel, Isaac Ojodale, N. Florence, J. Oniemola, Mayowa
Staphylococcus aureus is one of the prominent causes of hospital-acquired bacteremia. Despite the availability of anti-staphylococcal antibiotics, hospital acquired S. aureus bacteremia is still a major problem with considerable morbidity and mortality. Therefore, the aim of this study was to isolate, identify and determine the Antibiotics susceptibility profile of Staphylococcus aureus from the surfaces of surgical equipment and environment of major public and private hospitals in Lokoja, Kogi State, Nigeria using colonial characteristics, microscopy and conventional biochemical techniques. The Antibiotics susceptibility profile of the isolates was determined in accordance with the Guidelines of Clinical and Laboratory Standard Institute (CLSI). A total of three hundred and fifty (350) swab samples comprising of fourty (40) from surgical equipment and three hundred and ten (310) from the environment were collected from three (3) different public and private hospitals within Lokoja metropolis. The results obtained showed that 110(31.4%) of samples from the hospital environment were confirmed positive for Staphylococcus aureus with Hospital A constituting 30(8.6%), Hospital B had 59(16.8%) and Hospital C recorded 21 (6.0%). Of the 19 selected S. aureus isolates for antimicrobial susceptibility screening, 10.52% and 5.26% were intermediately resistant to Norfloxacin and Chloramphenicol respectively. Furthermore, the screened S. aureus isolates showed 100% susceptible to Ciprofloxacin, Gentamicin and Erythromycin; 94.73% susceptible to Chloramphenicol and 89.47% susceptible to Levoflaxin. The result also revealed 100% resistance to Penicillin and 15.78% resistance to Rifampicin. The high presence of Staphylococcus aureus in the hospital environment is a potential threat to the health of the patients and the public as this organism has been implicated in several human diseases, especially hospital- acquired bacteremia. Therefore, improved personal and public hygienic practices within the hospitals are required to reduce the high presence of S. aureus and other pathogenic microorganisms.
{"title":"Phenotypic Identification and Antibiotics Susceptibility Profile of Staphylococcus aureus from Surgical Equipment and Hospital Environment in Lokoja, Kogi State, Nigeria","authors":"Makolo, Daniel, Isaac Ojodale, N. Florence, J. Oniemola, Mayowa","doi":"10.47430/ujmr.2272.002","DOIUrl":"https://doi.org/10.47430/ujmr.2272.002","url":null,"abstract":"Staphylococcus aureus is one of the prominent causes of hospital-acquired bacteremia. Despite the availability of anti-staphylococcal antibiotics, hospital acquired S. aureus bacteremia is still a major problem with considerable morbidity and mortality. Therefore, the aim of this study was to isolate, identify and determine the Antibiotics susceptibility profile of Staphylococcus aureus from the surfaces of surgical equipment and environment of major public and private hospitals in Lokoja, Kogi State, Nigeria using colonial characteristics, microscopy and conventional biochemical techniques. The Antibiotics susceptibility profile of the isolates was determined in accordance with the Guidelines of Clinical and Laboratory Standard Institute (CLSI). A total of three hundred and fifty (350) swab samples comprising of fourty (40) from surgical equipment and three hundred and ten (310) from the environment were collected from three (3) different public and private hospitals within Lokoja metropolis. The results obtained showed that 110(31.4%) of samples from the hospital environment were confirmed positive for Staphylococcus aureus with Hospital A constituting 30(8.6%), Hospital B had 59(16.8%) and Hospital C recorded 21 (6.0%). Of the 19 selected S. aureus isolates for antimicrobial susceptibility screening, 10.52% and 5.26% were intermediately resistant to Norfloxacin and Chloramphenicol respectively. Furthermore, the screened S. aureus isolates showed 100% susceptible to Ciprofloxacin, Gentamicin and Erythromycin; 94.73% susceptible to Chloramphenicol and 89.47% susceptible to Levoflaxin. The result also revealed 100% resistance to Penicillin and 15.78% resistance to Rifampicin. The high presence of Staphylococcus aureus in the hospital environment is a potential threat to the health of the patients and the public as this organism has been implicated in several human diseases, especially hospital- acquired bacteremia. Therefore, improved personal and public hygienic practices within the hospitals are required to reduce the high presence of S. aureus and other pathogenic microorganisms.","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75308300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Idris, M. Abdullahi, H. Ahmad, M. A. Garga, N. Ibrahim, R. Agyo-Likita, A. Mohammad, I. Bako, I. M. Yusuf
This study compared the effects of chicken dropping, NPK fertilizer, and Jatropha seed cake on the growth of bush okro (Corchorus olitorious L.), as well as its proximate and mineral composition. In a completely randomized design with three fertilizer treatments at four levels, soil samples were homogenized with 1, 2, 4, and 8 g/bag each of Jatropha seed cake, chicken dropping, and NPK fertilizer for the growth of bush okra. Standard techniques were used to assess the compositions of the proximate and minerals. The highest percentages of ash, lipid, fiber, nitrogen, crude protein, and moisture observed in Jatropha seed cake were: 16.5% (4g/bag), 3.5% (8g/bag), 11.5% (4g/bag), 1.15% (1g/bag), 7.18% (1g/bag), and 7% (8g/bag), NPK fertilizer: 21.5% (1g/bag), 3.0% (2g/bag), 10.5% (1g/bag), 1.27% (1g/bag), 7.96% (1g/bag), and 7.5% (2g/bag), Chicken dropping: 20.5% (8g/bag), 3.5% (4g/bag), 10% (2g/bag), 1.12% (8g/bag), 7% (8g/bag), and 6.5% (2 and 8g/bag) respectively.. All of the minerals that were examined (Na, K, Ca, and Mg) were found to be present in modest amounts. The composition of Corchorus olitorious L. proximate and mineral components were not significantly (p>0.05) affected by fertilizer types. The study's findings indicate that the application of Jatropha seed cake, which is comparable to NPK fertilizer and chicken droppings at 8 g/bag, accelerates the growth of C. olitorious. The findings support the use of Jatropha seed cake as a source of organic fertilizer, which may aid in the present need for food that is produced.
{"title":"The Comparative Study of the Proximate and Mineral Composition of Bush Okro (Corchorus olitorious L.) under the Influence of Jatropha Seed Cake, Chicken droppings and NPK fertilizer","authors":"M. Idris, M. Abdullahi, H. Ahmad, M. A. Garga, N. Ibrahim, R. Agyo-Likita, A. Mohammad, I. Bako, I. M. Yusuf","doi":"10.47430/ujmr.2272.005","DOIUrl":"https://doi.org/10.47430/ujmr.2272.005","url":null,"abstract":"This study compared the effects of chicken dropping, NPK fertilizer, and Jatropha seed cake on the growth of bush okro (Corchorus olitorious L.), as well as its proximate and mineral composition. In a completely randomized design with three fertilizer treatments at four levels, soil samples were homogenized with 1, 2, 4, and 8 g/bag each of Jatropha seed cake, chicken dropping, and NPK fertilizer for the growth of bush okra. Standard techniques were used to assess the compositions of the proximate and minerals. The highest percentages of ash, lipid, fiber, nitrogen, crude protein, and moisture observed in Jatropha seed cake were: 16.5% (4g/bag), 3.5% (8g/bag), 11.5% (4g/bag), 1.15% (1g/bag), 7.18% (1g/bag), and 7% (8g/bag), NPK fertilizer: 21.5% (1g/bag), 3.0% (2g/bag), 10.5% (1g/bag), 1.27% (1g/bag), 7.96% (1g/bag), and 7.5% (2g/bag), Chicken dropping: 20.5% (8g/bag), 3.5% (4g/bag), 10% (2g/bag), 1.12% (8g/bag), 7% (8g/bag), and 6.5% (2 and 8g/bag) respectively.. All of the minerals that were examined (Na, K, Ca, and Mg) were found to be present in modest amounts. The composition of Corchorus olitorious L. proximate and mineral components were not significantly (p>0.05) affected by fertilizer types. The study's findings indicate that the application of Jatropha seed cake, which is comparable to NPK fertilizer and chicken droppings at 8 g/bag, accelerates the growth of C. olitorious. The findings support the use of Jatropha seed cake as a source of organic fertilizer, which may aid in the present need for food that is produced.","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78098976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The health risks associated with ingesting food contaminated with mycotoxins, particularly aflatoxin-contaminated staple foods like maize and other cereals, have been widely studied. However, there is little knowledge about the role of inhalation of pathogenic fungi as bioaerosols in contaminated air from handling crops as an occupational health risk. This paper presents a study aimed at determining the level of airborne aflatoxin-producing fungi in the indoor air of grain stores in the Giwa community of Kaduna State. Indoor air was sampled using the settling plate technique from grain stores, warehouses and living rooms. Metrological data of the studied area were collected from the Institute of Agricultural Research, ABU, Zaria. Airborne mycofloral concentrations were determined, and colonies of Aspergillus flavus were identified. The isolates were screened for aflatoxin production on Neutral Red Desiccated Coconut Agar (NRDCA). Selected aflatoxin-producing fungal isolates were screened for the presence of aflD (nor-1), aflM (ver-1) and AflR genes by PCR. Sampling was done once every month from October to December 2020. Mycofloral concentrations were in the range of 2.77x103−4.05x103 and 1.55x103−2.17x103CFUm-3for grain stores and living rooms respectively. A total of twelve (12) strains of A. flavus were isolated from the indoor air of the grain stores and warehouses while none was obtained from the living room. Eleven (11) isolates were confirmed to be aflatoxigenic on NRDCA, presenting 30 CFUm-3 of the indoor air mycofloral composition. The aflD, aflM and aflR were amplified with aflD being the most detected gene from all the aflatoxin-producing mould isolates of Aspergillus species. The mycofloral concentrations in the grain stores were higher than those in the living room and, in all the sampling sites, exceeded the limit of the total mycofloral concentration of 500 CFUm-3 for agricultural and industrial environments. There were significant differences (p<0.05) in the indoor mycofloral concentrations between the grain stores/warehouses and the living room. The presence of aflatoxigenic strains of A. flavus in the stores indicates that grain handlers and traders are at risk of occupational exposure to aflatoxigenic fungi and aflatoxins. Hence, they should wear protective materials for their safety while working in such stores.
{"title":"Evaluation of Aflatoxin-Producing Fungi in Indoor Air of Warehouses and Houses of Farmers in Giwa, Kaduna State Nigeria","authors":"Abdurrazaq, M., Tijjani, M.B., Atta, H.I.","doi":"10.47430/ujmr.2272.012","DOIUrl":"https://doi.org/10.47430/ujmr.2272.012","url":null,"abstract":"The health risks associated with ingesting food contaminated with mycotoxins, particularly aflatoxin-contaminated staple foods like maize and other cereals, have been widely studied. However, there is little knowledge about the role of inhalation of pathogenic fungi as bioaerosols in contaminated air from handling crops as an occupational health risk. This paper presents a study aimed at determining the level of airborne aflatoxin-producing fungi in the indoor air of grain stores in the Giwa community of Kaduna State. Indoor air was sampled using the settling plate technique from grain stores, warehouses and living rooms. Metrological data of the studied area were collected from the Institute of Agricultural Research, ABU, Zaria. Airborne mycofloral concentrations were determined, and colonies of Aspergillus flavus were identified. The isolates were screened for aflatoxin production on Neutral Red Desiccated Coconut Agar (NRDCA). Selected aflatoxin-producing fungal isolates were screened for the presence of aflD (nor-1), aflM (ver-1) and AflR genes by PCR. Sampling was done once every month from October to December 2020. Mycofloral concentrations were in the range of 2.77x103−4.05x103 and 1.55x103−2.17x103CFUm-3for grain stores and living rooms respectively. A total of twelve (12) strains of A. flavus were isolated from the indoor air of the grain stores and warehouses while none was obtained from the living room. Eleven (11) isolates were confirmed to be aflatoxigenic on NRDCA, presenting 30 CFUm-3 of the indoor air mycofloral composition. The aflD, aflM and aflR were amplified with aflD being the most detected gene from all the aflatoxin-producing mould isolates of Aspergillus species. The mycofloral concentrations in the grain stores were higher than those in the living room and, in all the sampling sites, exceeded the limit of the total mycofloral concentration of 500 CFUm-3 for agricultural and industrial environments. There were significant differences (p<0.05) in the indoor mycofloral concentrations between the grain stores/warehouses and the living room. The presence of aflatoxigenic strains of A. flavus in the stores indicates that grain handlers and traders are at risk of occupational exposure to aflatoxigenic fungi and aflatoxins. Hence, they should wear protective materials for their safety while working in such stores.","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73722603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Schiff base readily form complex with metal ions and the resultant complexes especially from transition metals were extensively used in many fields of human endeavor including antimicrobial therapy. However, the potentials of Lanthanide-Schiff base complexes as an antimicrobial agent have not been adequately studied. This study aims to synthesize and determine the antimicrobial activity of Bis-salicylaldehyde ethylenediamine Schiff Base and its Lanthanoids (III) Complexes. The Dysprosium (III), Gadolinium (III), Neodymium (III) and Samarium (III) complexes of Schiff base derived from Salicylaldehyde and ethylenediamine were synthesized under reflux condition in ethanol and their antimicrobial activity were determined using disc diffusion method. Bis-salicylaldehyde ethylenediamine was a shiny crystalline yellow with a yield of 93% and its complexes were various sheds of yellow ranges from pale to dark, with percentage yield between 94-98%. The Bis-salicylaldehyde ethylenediamine had good activity against C. albican (32mm at 100mg/ml), A. niger (20mm at 100mg/ml) and Fusarium Spp (17mm at 100mg/ml) but its complexes had low (07-11mm at 100mg/ml) or no (06mm at 100mg/ml) activity against all the fungal isolates. The antibacterial activity of the Bis-salicylaldehyde ethylenediamine against E. coli (8mm at 100mg/ml), Pseudomonas (6mm at 100mg/ml) and Klebsiella (13mm at 100mg/ml) was lower than that of Ampiclox (between 17 to 20mm) and even though, all its Lanthanoid (III) complexes between 8 to 14mm at 100mg/ml) had higher antibacterial activity than the Bis-salicylaldehydeethylenediamine but still the activity of Lanthanoid (III) complexes was lower than that of Ampiclox. Considering the in-vitro antimicrobial activity exhibited by Bis-salicylaldehyde ethylenediamine and its Lanthanoid (III) complexes against bacterial and fungal isolates, both will not be potential antibacterial agent but Bis-salicylaldehyde ethylenediamine could be pontential antifungal agent. We therefore, recommend further researches geared toward exploring its full potentials for antifungal therapy.
{"title":"Antimicrobial activity of Bis-salicylaldehyde Ethylenediamine Schiff Base and Its Lanthanoids (III) Complexes","authors":"Mahmud, S.D., Birnin-Yauri, U.A., Liman, M.G., Manga, S.B.","doi":"10.47430/ujmr.2272.013","DOIUrl":"https://doi.org/10.47430/ujmr.2272.013","url":null,"abstract":"Schiff base readily form complex with metal ions and the resultant complexes especially from transition metals were extensively used in many fields of human endeavor including antimicrobial therapy. However, the potentials of Lanthanide-Schiff base complexes as an antimicrobial agent have not been adequately studied. This study aims to synthesize and determine the antimicrobial activity of Bis-salicylaldehyde ethylenediamine Schiff Base and its Lanthanoids (III) Complexes. The Dysprosium (III), Gadolinium (III), Neodymium (III) and Samarium (III) complexes of Schiff base derived from Salicylaldehyde and ethylenediamine were synthesized under reflux condition in ethanol and their antimicrobial activity were determined using disc diffusion method. Bis-salicylaldehyde ethylenediamine was a shiny crystalline yellow with a yield of 93% and its complexes were various sheds of yellow ranges from pale to dark, with percentage yield between 94-98%. The Bis-salicylaldehyde ethylenediamine had good activity against C. albican (32mm at 100mg/ml), A. niger (20mm at 100mg/ml) and Fusarium Spp (17mm at 100mg/ml) but its complexes had low (07-11mm at 100mg/ml) or no (06mm at 100mg/ml) activity against all the fungal isolates. The antibacterial activity of the Bis-salicylaldehyde ethylenediamine against E. coli (8mm at 100mg/ml), Pseudomonas (6mm at 100mg/ml) and Klebsiella (13mm at 100mg/ml) was lower than that of Ampiclox (between 17 to 20mm) and even though, all its Lanthanoid (III) complexes between 8 to 14mm at 100mg/ml) had higher antibacterial activity than the Bis-salicylaldehydeethylenediamine but still the activity of Lanthanoid (III) complexes was lower than that of Ampiclox. Considering the in-vitro antimicrobial activity exhibited by Bis-salicylaldehyde ethylenediamine and its Lanthanoid (III) complexes against bacterial and fungal isolates, both will not be potential antibacterial agent but Bis-salicylaldehyde ethylenediamine could be pontential antifungal agent. We therefore, recommend further researches geared toward exploring its full potentials for antifungal therapy.","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88321905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The amount of environmental contamination brought on by the careless disposal of plastic garbage has increased to 400 million tons every year on a global scale. These synthetically generated traditional polymers are not easily biodegradable. This work was therefore undertaken to isolate Bacillus subtilis from soil with potential to produce bioplastic: Poly-ß-hydroxybutyrate (PHB). Samples of soil were collected from various locations (BG = Botanical Garden, FARD = Fine Art Refuse Dumpsite, SHRD = Suleiman Hall Refuse Dumpsite, FVM = Faculty of Veterinary Medicine animal paddock) within Ahmadu Bello University, Zaria, Nigeria. Spread plate technique was used to isolate B. subtilis on nutrient agar, and the isolates' cultural, morphological, and biochemical properties were identified. The isolates of B. subtilis were screened for PHB production using two different methods: the plate assay method and slide technique using Sudan black B dye. The PHB was then produced using submerged fermentation with watermelon peel as sole source of carbon in the production medium. The PHB was extracted using Sodium-hypochlorite method and the quality of the PHB was determined using FT-IR analysis. Four isolates of Bacillus subtilis were obtained from the soil samples (50 %), one each out of the two samples (50 %) per unit location (BG2, FARD3, SHRD2, and FVM4). The screening revealed that all the isolates were PHB producers. The B. subtilis isolate SHRD2 from the students’ dormitory was found to produce the highest PHB yield of 0.98 g/L from the watermelon substrate, whereas isolate from the animal paddock (FVM4) yielded the lowest quantity of the PHB (0.12 g /L). The biopolymer's (bioplastic) identity was confirmed to be PHB based on the peaks in the FT-IR spectra, which displayed wave numbers for a variety of functional groups, including -O-H, C-H, C-O, and C=O. It was concluded that the local isolates of B. subtilis have potentials for PHB production using watermelon peels as source of carbon and energy.
{"title":"Production of Bioplastic by Local Strains of Bacillus subtilis using Watermelon Peels as Substrate","authors":"Musa, B., Ado, S.A., Joseph, G.L., Hussain, I.M., Sulaiman, M.A., Tijjani, M.B., Charanchi, A.S.","doi":"10.47430/ujmr.2272.007","DOIUrl":"https://doi.org/10.47430/ujmr.2272.007","url":null,"abstract":"The amount of environmental contamination brought on by the careless disposal of plastic garbage has increased to 400 million tons every year on a global scale. These synthetically generated traditional polymers are not easily biodegradable. This work was therefore undertaken to isolate Bacillus subtilis from soil with potential to produce bioplastic: Poly-ß-hydroxybutyrate (PHB). Samples of soil were collected from various locations (BG = Botanical Garden, FARD = Fine Art Refuse Dumpsite, SHRD = Suleiman Hall Refuse Dumpsite, FVM = Faculty of Veterinary Medicine animal paddock) within Ahmadu Bello University, Zaria, Nigeria. Spread plate technique was used to isolate B. subtilis on nutrient agar, and the isolates' cultural, morphological, and biochemical properties were identified. The isolates of B. subtilis were screened for PHB production using two different methods: the plate assay method and slide technique using Sudan black B dye. The PHB was then produced using submerged fermentation with watermelon peel as sole source of carbon in the production medium. The PHB was extracted using Sodium-hypochlorite method and the quality of the PHB was determined using FT-IR analysis. Four isolates of Bacillus subtilis were obtained from the soil samples (50 %), one each out of the two samples (50 %) per unit location (BG2, FARD3, SHRD2, and FVM4). The screening revealed that all the isolates were PHB producers. The B. subtilis isolate SHRD2 from the students’ dormitory was found to produce the highest PHB yield of 0.98 g/L from the watermelon substrate, whereas isolate from the animal paddock (FVM4) yielded the lowest quantity of the PHB (0.12 g /L). The biopolymer's (bioplastic) identity was confirmed to be PHB based on the peaks in the FT-IR spectra, which displayed wave numbers for a variety of functional groups, including -O-H, C-H, C-O, and C=O. It was concluded that the local isolates of B. subtilis have potentials for PHB production using watermelon peels as source of carbon and energy.","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"158 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76815044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kaolin is a naturally occurring raw material for several industrial applications. Moreover, the economy of using Kaolin has some restrictions due to presence of iron-bearing impurities in form of oxides and hydroxides. This has a negative effect on the whiteness and refractoriness of some products and the production of high purity ceramics. The application of microbial leaching has been regarded as cheap and bio-friendly than conventional methods, hence this study investigates the isolation of potential iron reducing bacteria capable of providing efficient bioleaching. Bacteria isolation was carried out using serial dilution and plating techniquesResult of molecular, morphological and biochemical analyses reveal the presence of many bacterial isolates that includes Acinobacter spp., Aeromonas spp., Clostridium spp., Klebsiella spp., Pseudomonas spp. and Sporanaerobacter spp. Aeromonas and Sporanaerobacter species were identified as more closely related to the Pseudomonas and Geobacter species that are reported to be good iron reducing bacteria. This may suggest a possible candidate strain that could provide efficient bioleaching activity. Result of X-ray Fluorescence spectrometry of Kaolin prior to isolation and identification of indigenous bacteria was found to contain the following oxides; Fe2O3, SiO2, A12O3, MgO, P2O5, SO3, TiO2, MnO and CaO which confirmed the identity of Kaolin samples collected. The highest oxide was found to be SiO2.
{"title":"Identification of Iron Reducing Bacteria from Kaolin of Different Mining Sites in Northern Nigeria for Potential Bioleaching Activity","authors":"12 B.M, A. H. Kawo, S. Yahaya","doi":"10.47430/ujmr.2272.011","DOIUrl":"https://doi.org/10.47430/ujmr.2272.011","url":null,"abstract":"Kaolin is a naturally occurring raw material for several industrial applications. Moreover, the economy of using Kaolin has some restrictions due to presence of iron-bearing impurities in form of oxides and hydroxides. This has a negative effect on the whiteness and refractoriness of some products and the production of high purity ceramics. The application of microbial leaching has been regarded as cheap and bio-friendly than conventional methods, hence this study investigates the isolation of potential iron reducing bacteria capable of providing efficient bioleaching. Bacteria isolation was carried out using serial dilution and plating techniquesResult of molecular, morphological and biochemical analyses reveal the presence of many bacterial isolates that includes Acinobacter spp., Aeromonas spp., Clostridium spp., Klebsiella spp., Pseudomonas spp. and Sporanaerobacter spp. Aeromonas and Sporanaerobacter species were identified as more closely related to the Pseudomonas and Geobacter species that are reported to be good iron reducing bacteria. This may suggest a possible candidate strain that could provide efficient bioleaching activity. Result of X-ray Fluorescence spectrometry of Kaolin prior to isolation and identification of indigenous bacteria was found to contain the following oxides; Fe2O3, SiO2, A12O3, MgO, P2O5, SO3, TiO2, MnO and CaO which confirmed the identity of Kaolin samples collected. The highest oxide was found to be SiO2.","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80292774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Musa, B., Zangina, B. U., Ado, S. A., Hussaini, I. M., Madika, A., Aliyu, M. S.
There is an increasing demand for high fructose corn syrup as an alternative to glucose especially for use by people with diabetes due to its low glycemic index. The high cost of this product coupled with its high demand has attracted the attention of many researchers to search for an alternative and sustainable production route. Therefore, the focus of this work was isolating and screening Aspergillus niger and Bacillus coagulans as possible producers of amylase and glucose isomerase respectively, which are needed to produce high fructose corn syrup. Nine (9) samples of loamy soil were taken from three (3) separate locations (BG = Botanical Garden, RD = Refuse Dumpsite, FB = Flower Bed) to isolate the bacterium and fungus. Based on the lacto-phenol cotton blue stain preparation under a microscope, colonies that were thought to be Aspergillus niger colonies were observed and further identified. Cultural, microscopic, and biochemical characteristics were used to confirm the identification of colonies that were thought to be Bacillus coagulans. The probable isolates of Aspergillus niger and Bacillus coagulans were then screened for amylase and glucose isomerase production respectively. Out of the total of nine (9) soil samples analysed, 5 (55.6 %) were positive for Aspergillus niger. A higher isolation rate of Aspergillus niger, 2 (66.7 %) was recorded in soil samples from botanical garden (BG) and flower bed (FB). The lowest isolation rate, 1 (33.3 %) was observed in soil obtained from the refuse dump. Out of all the isolates screened for their potential to produce amylase, the isolate from the botanical garden showed the highest zone of starch hydrolysis (28 mm), and the isolate from the flower bed showed the least zone of hydrolysis of starch (13 mm). On the other hand, out of the nine (9) soil samples analysed, 6 (66.7 %) were positive for Bacillus coagulans and the highest occurrence of Bacillus coagulans, 3 (100 %) was recorded with soil from botanical garden (BG1), while the least occurrence, 1 (33.3 %) was observed in soil from flower bed (FB2). The probable isolates of Bacillus coagulans screened for glucose isomerase production revealed that all the six (6) isolates produced glucose isomerase, with isolate from refuse dump (RD2) producing the highest concentration of glucose isomerase (4.7014 g/L).
{"title":"Isolation and Screening of Aspergillus niger and Bacillus coagulans as Potential Candidates for Amylase and Glucose Isomerase Production","authors":"Musa, B., Zangina, B. U., Ado, S. A., Hussaini, I. M., Madika, A., Aliyu, M. S.","doi":"10.47430/ujmr.2272.008","DOIUrl":"https://doi.org/10.47430/ujmr.2272.008","url":null,"abstract":"There is an increasing demand for high fructose corn syrup as an alternative to glucose especially for use by people with diabetes due to its low glycemic index. The high cost of this product coupled with its high demand has attracted the attention of many researchers to search for an alternative and sustainable production route. Therefore, the focus of this work was isolating and screening Aspergillus niger and Bacillus coagulans as possible producers of amylase and glucose isomerase respectively, which are needed to produce high fructose corn syrup. Nine (9) samples of loamy soil were taken from three (3) separate locations (BG = Botanical Garden, RD = Refuse Dumpsite, FB = Flower Bed) to isolate the bacterium and fungus. Based on the lacto-phenol cotton blue stain preparation under a microscope, colonies that were thought to be Aspergillus niger colonies were observed and further identified. Cultural, microscopic, and biochemical characteristics were used to confirm the identification of colonies that were thought to be Bacillus coagulans. The probable isolates of Aspergillus niger and Bacillus coagulans were then screened for amylase and glucose isomerase production respectively. Out of the total of nine (9) soil samples analysed, 5 (55.6 %) were positive for Aspergillus niger. A higher isolation rate of Aspergillus niger, 2 (66.7 %) was recorded in soil samples from botanical garden (BG) and flower bed (FB). The lowest isolation rate, 1 (33.3 %) was observed in soil obtained from the refuse dump. Out of all the isolates screened for their potential to produce amylase, the isolate from the botanical garden showed the highest zone of starch hydrolysis (28 mm), and the isolate from the flower bed showed the least zone of hydrolysis of starch (13 mm). On the other hand, out of the nine (9) soil samples analysed, 6 (66.7 %) were positive for Bacillus coagulans and the highest occurrence of Bacillus coagulans, 3 (100 %) was recorded with soil from botanical garden (BG1), while the least occurrence, 1 (33.3 %) was observed in soil from flower bed (FB2). The probable isolates of Bacillus coagulans screened for glucose isomerase production revealed that all the six (6) isolates produced glucose isomerase, with isolate from refuse dump (RD2) producing the highest concentration of glucose isomerase (4.7014 g/L).","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82693031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pounded yam is a staple food consumed in Makurdi, Benue State. The food is sold along major streets and roads in the town, as such; it is prone to bacterial contamination from different sources. The study was aimed at assessing the contamination and susceptibility pattern of bacteria isolated from pounded yam sold along major roads in Makurdi metropolis. One hundred and forty (140) samples of pounded yam were aseptically collected from seven food vending sites (Wurukum, Wadata, High level, Naka road, Otukpo road, Gboko road and North bank) and analyzed for bacterial contamination using standard microbiological procedures. The Kirby-Bauer disk diffusion method was used for the antibacterial susceptibility testing. The antibiotics tested were Septrin, Chloramphenicol, Sparfloxacin, Ciprofloxacin, Amoxicillin, Augmentin, Gentamycin, Pefloxacin, Tarivid and Streptomycin. If a bacterial isolate proved resistant to at least three of the tested antibiotics, it was deemed to be multi-drug resistant (MDR). A total of four species of bacteria which included Staphylococcus aureus (35.66%), Escherichia coli (18.18%), Klebsiella spp (11.20%) and Proteus spp (34.97%), were isolated from the pounded yam samples. The mean total viable count of all the samples; which ranges from 2.61x105cfu/g (Wadata) to 3.25x105cfu/g (Otukpo road) are within the acceptable limits for food. Staphylococcus spp, Escherichia coli and Proteus spp were multidrug resistant to four, six and three antibiotics respectively, according to the antibacterial susceptibility test. Staphylococcus spp showed 31.37% susceptibility to Septrin, 37.25% to Streptomycin and 33.33% of the Staphylococcus isolates were sensitive to Sparfloxacin and Amoxycillin. For Escherichia coli, more than 50% of the 26 Escherichia coli isolates showed resistance to Septrin (46.16%), Sparfloxacin (42.31%), Amoxicillin (42.31%), Gentamycin (38.46%), Perfloxacin ((46.15%) and Streptomycin (23.08%). Proteus spp showed 44.00% susceptibility to Chloramphenicol, 30.00% to Sparfloxacin and 40.00% to amoxicillin. Klebsiella spp showed 25.00% susceptibility to Amoxicillin only. The study identified that pounded yam sold along major roads in Makurdi metropolis are contaminated with bacteria of public health importance. Therefore hygienic way of food preparation is of utmost importance to prevent contamination of food with these organisms resulting to food borne diseases.
{"title":"Assessments of Contamination and Susceptibility Pattern of Bacteria Isolated from Pounded Yam Sold Along Major Roads in Makurdi Metropolis, Benue State, Nigeria","authors":"Womboh, S. B., Ajumobi, V. E., Ebute, P. A.","doi":"10.47430/ujmr.2272.009","DOIUrl":"https://doi.org/10.47430/ujmr.2272.009","url":null,"abstract":"Pounded yam is a staple food consumed in Makurdi, Benue State. The food is sold along major streets and roads in the town, as such; it is prone to bacterial contamination from different sources. The study was aimed at assessing the contamination and susceptibility pattern of bacteria isolated from pounded yam sold along major roads in Makurdi metropolis. One hundred and forty (140) samples of pounded yam were aseptically collected from seven food vending sites (Wurukum, Wadata, High level, Naka road, Otukpo road, Gboko road and North bank) and analyzed for bacterial contamination using standard microbiological procedures. The Kirby-Bauer disk diffusion method was used for the antibacterial susceptibility testing. The antibiotics tested were Septrin, Chloramphenicol, Sparfloxacin, Ciprofloxacin, Amoxicillin, Augmentin, Gentamycin, Pefloxacin, Tarivid and Streptomycin. If a bacterial isolate proved resistant to at least three of the tested antibiotics, it was deemed to be multi-drug resistant (MDR). A total of four species of bacteria which included Staphylococcus aureus (35.66%), Escherichia coli (18.18%), Klebsiella spp (11.20%) and Proteus spp (34.97%), were isolated from the pounded yam samples. The mean total viable count of all the samples; which ranges from 2.61x105cfu/g (Wadata) to 3.25x105cfu/g (Otukpo road) are within the acceptable limits for food. Staphylococcus spp, Escherichia coli and Proteus spp were multidrug resistant to four, six and three antibiotics respectively, according to the antibacterial susceptibility test. Staphylococcus spp showed 31.37% susceptibility to Septrin, 37.25% to Streptomycin and 33.33% of the Staphylococcus isolates were sensitive to Sparfloxacin and Amoxycillin. For Escherichia coli, more than 50% of the 26 Escherichia coli isolates showed resistance to Septrin (46.16%), Sparfloxacin (42.31%), Amoxicillin (42.31%), Gentamycin (38.46%), Perfloxacin ((46.15%) and Streptomycin (23.08%). Proteus spp showed 44.00% susceptibility to Chloramphenicol, 30.00% to Sparfloxacin and 40.00% to amoxicillin. Klebsiella spp showed 25.00% susceptibility to Amoxicillin only. The study identified that pounded yam sold along major roads in Makurdi metropolis are contaminated with bacteria of public health importance. Therefore hygienic way of food preparation is of utmost importance to prevent contamination of food with these organisms resulting to food borne diseases.","PeriodicalId":23463,"journal":{"name":"UMYU Journal of Microbiology Research (UJMR)","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88647439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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