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Focused ultrasonic transducer for aircraft icing detection 用于飞机结冰探测的聚焦超声波传感器。
IF 3.8 2区 物理与天体物理 Q1 ACOUSTICS Pub Date : 2024-11-12 DOI: 10.1016/j.ultras.2024.107509
Yan Wang , Yuan Wang , Siyu Chen , Chengxiang Zhu , Dawei Wu , Chunling Zhu , Xiyun Lu
Ultrasonic detection technique (UDT) serves as a pivotal method for monitoring aircraft icing conditions. However, the inherently porous and irregular shape of atmospheric ice leads to a pronounced attenuation of ultrasonic wave energy during propagation. Current ultrasonic transducers (UTs) fall short of meeting the requisite sensitivity and depth parameters for effective detection. This study proposes an innovative focused ultrasonic transducer (FUT) designed to extend the range of ice detection capabilities. Constructed using a 1–3 piezoelectric composite configuration, this FUT is characterized by its flexibility and slender profile. The focusing effect was accomplished through a deliberate bending mechanism. The FUT demonstrates its efficacy in detecting ice on aluminium skin surfaces. Furthermore, we validated the focusing effect and conducted a thorough optimization process. A comparative analysis between the FUT and traditional planar UTs revealed that the FUT enhances detection energy by approximately 30%, while also nearly doubling the detection range for glaze ice. These findings underscore the FUT’s promising potential for applications in the detection of substantial ice.
超声波探测技术(UDT)是监测飞机结冰状况的重要方法。然而,大气中的冰本身多孔且形状不规则,导致超声波能量在传播过程中明显衰减。目前的超声波传感器(UT)无法满足有效探测所需的灵敏度和深度参数。本研究提出了一种创新的聚焦超声波换能器 (FUT),旨在扩大冰探测能力的范围。这种 FUT 采用 1-3 级压电复合结构,具有柔韧性和细长外形的特点。聚焦效果是通过特意的弯曲机制实现的。FUT 证明了其在检测铝皮表面的冰层方面的功效。此外,我们还验证了聚焦效果,并进行了全面优化。FUT 与传统平面 UT 的对比分析表明,FUT 可将检测能量提高约 30%,同时还将釉冰的检测范围扩大了近一倍。这些发现凸显了 FUT 在大量冰检测方面的应用潜力。
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引用次数: 0
Improving the signal-to-noise ratio of the laser ultrasonic synthetic aperture focusing technique to detect submillimeter internal defects using echo array similarity 利用回波阵列相似性提高激光超声合成孔径聚焦技术的信噪比,以探测亚毫米级内部缺陷。
IF 3.8 2区 物理与天体物理 Q1 ACOUSTICS Pub Date : 2024-11-09 DOI: 10.1016/j.ultras.2024.107513
Huabin He, Jianguo He, Zhihui Xia, Kaihua Sun, Chao Wang, Qian Liu
Laser ultrasonic imaging is a promising technique for structural health monitoring because it is noncontact and nondestructive. However, this technique will only find more industrial applications if it has a high signal-to-noise ratio (SNR) and short data acquisition time. In existing delay-and-sum algorithms, such as the synthetic aperture focusing technique (SAFT) and the total focusing method, a higher SNR requires more A-scan signals, which mean a longer data acquisition time. It is difficult for these algorithms to consider these two aspects simultaneously. Thus, in this study, we propose a post-processing algorithm that extracts neglected information from laser ultrasonic B-scan data to improve the SNR of the SAFT without increasing the data acquisition time. The SNR was increased by multiplying the SAFT image intensity with the echo array similarity defined using the directivity and echo shape information of laser ultrasound. In experiments, SNR was increased from 4.1 dB to 31.3 dB for two submillimeter defects having a diameter of 0.5 mm and depth of 15 mm. Deeper defects can be detected because of the improved SNR. In this study, two submillimeter defects with a depth of 30 mm were detected. Compared with existing delay-and-sum algorithms, the proposed algorithm performs well in terms of both SNR and data acquisition time, which can promote its use in more industrial applications.
激光超声波成像技术具有非接触和无损的特点,是一种很有前途的结构健康监测技术。然而,只有在信噪比(SNR)高和数据采集时间短的情况下,这种技术才能得到更多的工业应用。在现有的延迟和算法中,如合成孔径聚焦技术(SAFT)和全聚焦法,较高的信噪比需要更多的 A 扫描信号,这意味着较长的数据采集时间。这些算法很难同时考虑这两个方面。因此,在本研究中,我们提出了一种后处理算法,从激光超声 B 扫描数据中提取被忽略的信息,在不增加数据采集时间的情况下提高 SAFT 的信噪比。利用激光超声的指向性和回波形状信息定义的回波阵列相似度乘以 SAFT 图像强度,从而提高信噪比。在实验中,对于两个直径为 0.5 毫米、深度为 15 毫米的亚毫米缺陷,信噪比从 4.1 分贝提高到 31.3 分贝。由于信噪比的提高,可以检测到更深的缺陷。在这项研究中,检测到了两个深度为 30 毫米的亚毫米缺陷。与现有的延迟求和算法相比,所提出的算法在信噪比和数据采集时间方面都有很好的表现,可以促进其在更多工业应用中的使用。
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引用次数: 0
Research on the second-harmonic focused ultrasonic device based on micro-bubble contrast agents 基于微气泡造影剂的二次谐波聚焦超声装置研究。
IF 3.8 2区 物理与天体物理 Q1 ACOUSTICS Pub Date : 2024-11-09 DOI: 10.1016/j.ultras.2024.107514
Qinglei Zeng , Yuetongxu Li , Zhaoyu Deng , Gutian Zhang , Chengwei Zhang , Haifeng Huang , Xiaozhou Liu
Non-invasive, accurate diagnosis and treatment have increasingly gained attention in medical research. The nonlinear response mechanism of ultrasound contrast agents and their medical application have become major topics in ultrasound imaging studies. This paper reports on a second-harmonic focused ultrasonic device based on micro-bubble contrast agents, which is designed to solve the problems associated with a weak second-harmonic intensity. A periodic array of circular holes is embedded in the center of a specifically shaped resin plate, and contrast agents are encapsulated in the circular holes using thin resin tape. The functional mechanism is theoretically explained and experimentally verified. This device enables second-harmonic ultrasound imaging with a higher ultrasonic lateral resolution and signal-to-noise ratio than the conventional system without the device.
无创、准确的诊断和治疗越来越受到医学研究的关注。超声造影剂的非线性响应机制及其医学应用已成为超声成像研究的主要课题。本文报告了一种基于微气泡造影剂的二次谐波聚焦超声装置,旨在解决二次谐波强度较弱的相关问题。在特定形状的树脂板中心嵌入了周期性的圆孔阵列,并使用薄树脂带将造影剂封装在圆孔中。该装置的功能机制得到了理论解释和实验验证。与不使用该装置的传统系统相比,该装置能以更高的超声横向分辨率和信噪比进行二次谐波超声成像。
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引用次数: 0
Optimal transport assisted full waveform inversion for multiparameter imaging of soft tissues in ultrasound computed tomography 超声计算机断层扫描中软组织多参数成像的最佳传输辅助全波形反演
IF 3.8 2区 物理与天体物理 Q1 ACOUSTICS Pub Date : 2024-11-08 DOI: 10.1016/j.ultras.2024.107505
Xiaoqing Wu , Yubing Li , Chang Su , Panpan Li , Weijun Lin
Ultrasound computed tomography (USCT) has emerged as a promising platform for imaging tissue properties, offering non-ionizing and operator-independent capabilities. In this work, we demonstrate the feasibility of obtaining quantitative images of multiple acoustic parameters (sound speed and impedance) for soft tissues using full waveform inversion (FWI), which are justified with both numerical and experimental cases. A 3D reconstruction based on a series of 2D slice images is presented for the experimental case of ex vivo soft tissues. To improve the robustness of the reconstruction process, a hierarchical FWI strategy is adopted, gradually iterating from low to high frequencies. In parallel, we employ a graph-space optimal transport misfit function, avoiding convergence into local minima and minimizing inversion artifacts caused by skin-related supercritical reflections. Our method first carries out sound speed inversion based on transmitted waves in the low and middle frequency bands, and then uses all types of waves in the high frequency band for simultaneous inversion of both sound speed and impedance. Compared to conventional strategies, the proposed approach can accurately reconstruct physical models consistent with the actual soft tissue sample. These high-resolution ultrasound images of acoustic parameters are promising to allow for quantitative differentiation among different types of tissues (e.g., muscles and fats). These results have significant implications for advancing our understanding of tissue properties and for potentially contributing to disease diagnosis through USCT, which is a flexible and cost-effective alternative to X-ray computed tomography or magnetic resonance imaging at no significant sacrifices for resolution.
超声计算机断层扫描(USCT)已经成为一种很有前途的组织特性成像平台,提供非电离和独立于操作员的能力。在这项工作中,我们证明了使用全波形反演(FWI)获得软组织多个声学参数(声速和阻抗)定量图像的可行性,并通过数值和实验案例证明了这一点。针对离体软组织实验案例,提出了一种基于一系列二维切片图像的三维重建方法。为了提高重建过程的鲁棒性,采用分层FWI策略,从低频到高频逐步迭代。同时,我们采用了图空间最优输运错拟合函数,避免了收敛到局部极小值,并最小化了由与皮肤相关的超临界反射引起的反演伪影。我们的方法首先基于中低频段的透射波进行声速反演,然后利用高频段的所有类型的波同时反演声速和阻抗。与传统方法相比,该方法可以准确地重建与实际软组织样品一致的物理模型。这些声学参数的高分辨率超声图像有望允许在不同类型的组织(例如肌肉和脂肪)之间进行定量区分。这些结果对于提高我们对组织特性的理解具有重要意义,并可能有助于通过USCT进行疾病诊断,USCT是x射线计算机断层扫描或磁共振成像的一种灵活且经济的替代方案,且不会显著牺牲分辨率。
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引用次数: 0
Ultrafast 3D synthetic aperture imaging with Hadamard-encoded aperiodic interval codes and aperiodic sparse arrays with separate transmitters and receivers 利用哈达玛编码的非周期性间隔码和具有独立发射器和接收器的非周期性稀疏阵列进行超快三维合成孔径成像。
IF 3.8 2区 物理与天体物理 Q1 ACOUSTICS Pub Date : 2024-11-08 DOI: 10.1016/j.ultras.2024.107497
Tarek Kaddoura, Mohammad Hadi Masoumi, Roger Zemp
3D synthetic aperture (SA) imaging of volumes can be obtained using sparse 2D ultrasound arrays. However, even with just 256 elements, the volumetric imaging rate can be relatively slow due to having to transmit on each element in succession. Hadamard Aperiodic Interval (HAPI) codes can be used to image the full SA dataset in one extended transmit to speed up the synthetic aperture imaging, but their long nature produces large deadzones if the same elements are used as both transmitters and receivers. In this simulation study, we use a 2D Costas sparse array with separate transmitters and receivers to remedy the deadzone problem, and use it with the HAPI-coded imaging scheme to obtain fully transmit–receive focused, wide field-of-view 3D volumes with high-resolution and high SNR at ultrafast volumetric imaging rates of more than 500 volumes per second, almost nine times faster than non-coded SA imaging with the same imaging parameters. We show similar PSF performance compared to non-coded SA, and a 26 dB improvement in SNR with order-256 HAPI codes. We also present cyst simulations showing similar contrast for the HAPI-coded SA method compared to non-coded SA in the context of no noise, and improved contrast in the context of noise.
使用稀疏的二维超声阵列可以获得体积的三维合成孔径(SA)成像。然而,即使只有 256 个元素,由于必须在每个元素上连续传输,容积成像速度也会相对较慢。Hadamard Aperiodic Interval (HAPI) 编码可用于在一次扩展发射中对整个 SA 数据集成像,以加快合成孔径成像速度,但如果将相同的元素用作发射器和接收器,其长特性会产生较大的死区。在这项模拟研究中,我们使用了具有独立发射器和接收器的二维科斯塔斯稀疏阵列来解决死区问题,并将其与 HAPI 编码成像方案结合使用,以每秒超过 500 幅的超快体积成像速度获得了具有高分辨率和高信噪比的全发射-接收聚焦宽视场三维体积,其速度几乎是相同成像参数下非编码 SA 成像速度的九倍。与非编码 SA 相比,我们展示了相似的 PSF 性能,而使用阶次 256 HAPI 编码,信噪比提高了 26 分贝。我们还进行了模拟实验,结果显示在无噪声的情况下,HAPI 编码 SA 方法与非编码 SA 方法的对比度相似,而在有噪声的情况下,对比度有所提高。
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引用次数: 0
Generation of broadband airborne ultrasound using an Harmonic Acoustic Pneumatic Source 利用谐波声气动源产生宽带空中超声波。
IF 3.8 2区 物理与天体物理 Q1 ACOUSTICS Pub Date : 2024-11-08 DOI: 10.1016/j.ultras.2024.107494
Romain Rousseau, Pierre Grandjean, Nicolas Quaegebeur, Loïc Charlebois-Vachon, Philippe Micheau
This paper presents a new type of airborne transducer for generating broadband ultrasound with a high Sound Pressure Level (SPL). The concept is based on the Harmonic Acoustic Pneumatic Source (HAPS) that uses pressurized air in conjunction with a flow chopper made up of a rotating cage with slots connected to a specific exhaust. The fundamental frequency depends on the number of slots and the rotation speed of the cage. An analytical model of the HAPS coupled with a numerical model of the exhaust is used to predict the radiated acoustic pressure and to estimate the influence of dimensional parameters on pressure level generated by the source. Experiments are conducted with two cages: one with one slot in order to generate pulses periodically and one with 122 slots to generate periodic sound. The level of sound pressure is measured as a function of distance (0.004 to 0.5 m), the cage rotation (up to 11 krpm) and directivity (0 to 90°). For the fundamental frequency at 22 kHz, the maximum SPL of 150 dB (632 Pa rms) is /measured at 0.004 m, and decreases to 122 dB (35 Pa rms) at 0.5 m. At 0.5 m, the second and third harmonics can generate a SPL equal or greater than 115 dB above 22 kHz and up to 66 kHz. Discrepancies between the experiments results and numerical model are observed in terms of SPL, directivity and in-axis pressure.
本文介绍了一种用于产生高声压级(SPL)宽带超声波的新型机载换能器。该概念基于谐波声学气动源(HAPS),它使用加压空气与由旋转笼组成的斩流器,旋转笼上的槽与特定的排气管相连。基频取决于槽的数量和笼子的旋转速度。HAPS 的分析模型与排气的数值模型相结合,用于预测辐射声压,并估算尺寸参数对声源产生的压力水平的影响。实验使用了两个笼子:一个带有一个槽,用于产生周期性脉冲;另一个带有 122 个槽,用于产生周期性声音。测量的声压级是距离(0.004 至 0.5 米)、笼子旋转(最高 11 千转/分)和指向性(0 至 90°)的函数。对于 22 kHz 的基频,在 0.004 m 处测得的最大声压级为 150 dB(632 Pa rms),在 0.5 m 处降至 122 dB(35 Pa rms)。实验结果与数值模型在声压级、指向性和轴内压力方面存在差异。
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引用次数: 0
Ultrafast laser-enabled optoacoustic characterization of three-dimensional, nanoscopic interior features of microchips 利用超快激光对微型芯片的三维纳米内部特征进行光声表征。
IF 3.8 2区 物理与天体物理 Q1 ACOUSTICS Pub Date : 2024-11-06 DOI: 10.1016/j.ultras.2024.107510
Yi He , Guojie Luo , Jie Huang , Yehai Li , Hoon Sohn , Zhongqing Su
The recent advances in micromanufacturing have been pushing boundaries of the new generation of semiconductor devices, which, in the meantime, brings new challenges in the material and structural characterization – a key step to ensure the device quality through the micromanufacturing process. An ultrafast laser-enable optoacoustic characterization methodology is developed, targeting in situ calibration and delineation of the three-dimensional (3-D), nanoscopic interior features of opaque semiconductor chips. With the guidance of ultrafast electron–phonon coupling effect and velocity-perturbated optical interference, a femtosecond-laser pump–probe set-up based on Sagnac interferometer is configured to generate and acquire picosecond ultrasonic bulk waves (P-UBWs) traversing the microchips. The interior features of the microchips shift the phase of acquired P-UBW signals, reflected in the perturbed probe laser beam. The phase shifts are calibrated to compute signal correlation of P-UBW signals between different acquiring positions, whereby to delineate the interior features in an intuitive manner. The approach is experimentally validated by characterizing nanoscopic, invisible interior aurum(Au)-gratings with periodically varied depths in typical microchips. Results highlight that the 3-D nanoscopic features of the microchips can be revealed with a microscopic and a nanoscopic spatial resolution, respectively along the transverse and depth directions of the chip, where the Au-gratings become “visible” with a depth variance of a few tens of nanometers only. This proposed approach has provided a fast, nondestructive approach to “see” through an opaque microchip with a nanoscopic resolution.
微制造技术的最新进展推动了新一代半导体器件的发展,同时也给材料和结构表征带来了新的挑战,而材料和结构表征是通过微制造过程确保器件质量的关键步骤。针对不透明半导体芯片的三维(3-D)、纳米级内部特征的现场校准和划分,我们开发了一种超快激光光声表征方法。在超快电子-声子耦合效应和速度扰动光学干涉的指导下,基于萨格纳克干涉仪的飞秒激光泵浦-探针装置被配置用来产生和获取穿越微芯片的皮秒超声波(P-UBW)。微芯片的内部特征会移动所获取的 P-UBW 信号的相位,并反射到扰动探针激光束中。对相移进行校准,计算不同采集位置之间 P-UBW 信号的相关性,从而以直观的方式划分内部特征。通过对典型微芯片中深度周期性变化的纳米级不可见内部金(Au)光栅进行表征,对该方法进行了实验验证。结果表明,微芯片的三维纳米特征可以在芯片的横向和纵向分别以微米级和纳米级的空间分辨率显示出来,其中金槽的 "可见 "深度差异仅为几十纳米。这种拟议的方法提供了一种快速、无损的方法,能以纳米分辨率 "看透 "不透明的微芯片。
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引用次数: 0
An algorithm for multi-damage size estimation of composite laminates 复合材料层压板多损伤尺寸估算算法。
IF 3.8 2区 物理与天体物理 Q1 ACOUSTICS Pub Date : 2024-11-05 DOI: 10.1016/j.ultras.2024.107511
Zhongyan Jin , Qihong Zhou , Zeguang Pei , Ge Chen
Localization and size estimation of composite damage are challenging but essential for composite performance evaluation. This paper proposes a new methodology for the size estimation of multi-damage in composite laminates using Lamb wave technology. The pure A0 modal of Lamb wave is excited to avoid dispersion and multi-modal effects of Lamb wave. An extraction algorithm is introduced to obtain the first wave packet and time-of-flight. According to the results obtained by the extraction algorithm, the Bayesian-hybrid localization algorithm based on the reconstruction algorithm for probabilistic inspection of damage and modified delay-and-sum (MDAS) is performed to localize damages. The damage boundaries are obtained through convex enveloping a series of damage boundary points identified by MDAS. An adaptive Gaussian mixture model based on Akaike’s Information Criterion and Bayesian Information Criterion is designed to remove abnormal boundary points. The proposed method is numerically investigated and validated through multi-damage experiments. The results demonstrate that it can accurately estimate the locations and boundaries of multi-damage in composite laminates.
复合材料损伤的定位和尺寸估算具有挑战性,但对复合材料性能评估至关重要。本文提出了一种利用 Lamb 波技术估算复合材料层压板多损伤尺寸的新方法。本文激发了纯 A0 模态的 Lamb 波,以避免 Lamb 波的色散和多模态效应。引入了一种提取算法来获取第一波包和飞行时间。根据提取算法得到的结果,采用基于损伤概率检测重构算法和修正延迟和(MDAS)的贝叶斯混合定位算法对损伤进行定位。通过凸包络 MDAS 确定的一系列损伤边界点,获得损伤边界。设计了一种基于 Akaike 信息准则和贝叶斯信息准则的自适应高斯混合模型,以去除异常边界点。通过多损伤实验对所提出的方法进行了数值研究和验证。结果表明,该方法能准确估计复合材料层压板中多重损伤的位置和边界。
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引用次数: 0
Band edge modulation for high-performance LL-SAW resonators on LiNbO3/SiC by introducing an ultra-thin intermediate oxide layer 通过引入超薄中间氧化物层,在 LiNbO3/SiC 上实现高性能 LL-SAW 谐振器的带边调制。
IF 3.8 2区 物理与天体物理 Q1 ACOUSTICS Pub Date : 2024-11-04 DOI: 10.1016/j.ultras.2024.107508
Juxing He , Shibin Zhang , Pengcheng Zheng , Xiaoli Fang , Hulin Yao , Mijing Sun , Dongchen Sui , Yanlong Yao , Chongxi Song , Zheng Zhou , Xin Ou
With the exploding demand of rapid information transmission, high-frequency acoustic filtering devices are becoming an immediate need. Longitudinal leaky surface acoustic wave (LL-SAW) devices with unique advantages can be a promising platform. In this paper, we introduce a 100 nm intermediate oxide layer into the X-cut lithium niobate on silicon carbide (LiNbO3/SiC) to improve the in-band performance of LL-SAW resonators. First, the dispersion curves of the structures are analyzed by finite element method. In this part, we successfully interpret the intrinsic low quality factor (Q) of LL-SAW on LiNbO3/SiC in general design, and predict the enhancement of Q by introducing an intermediate oxide layer without degradation on spurious response. Then, one port resonators considered in the simulation are fabricated and measured. As a result, enhancements in Bode Q among the whole passband are confirmed. Compared with devices state of art, resonators with leading performances are demonstrated. The fabricated resonators have peak-valley admittance ratio of 63.87 dB, Bode Q of ∼300 at fr and ∼530 at far, keff2of 15.66 % and phase velocity of 6187.3 m/s. Additionally, the resonant frequency of SH1 mode shifts to higher frequency. This work enables the design of next generation high frequency mobile communication filters.
随着快速信息传输需求的不断增长,高频声学滤波设备已成为当务之急。具有独特优势的纵向泄漏表面声波(LL-SAW)器件是一个前景广阔的平台。本文在 X 切碳化硅铌酸锂(LiNbO3/SiC)中引入了 100 nm 的中间氧化层,以改善 LL-SAW 谐振器的带内性能。首先,我们用有限元法分析了结构的色散曲线。在这一部分,我们成功地解释了在一般设计中,LiNbO3/SiC 上的 LL-SAW 固有的低品质因数(Q),并预测了通过引入中间氧化物层来增强 Q 值,而不会降低杂散响应。然后,制作并测量了模拟中考虑的单端口谐振器。结果证实,整个通带的 Bode Q 值都有所提高。与现有器件相比,谐振器的性能处于领先地位。所制作的谐振器的峰谷导纳比为 63.87 dB,远近端 Bode Q 值分别为 ∼300 和 ∼530,keff2 为 15.66 %,相位速度为 6187.3 m/s。此外,SH1 模式的共振频率也变高了。这项工作有助于设计下一代高频移动通信滤波器。
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引用次数: 0
Low-intensity pulsed ultrasound reduces oxidative and endoplasmic reticulum stress in motor neuron cells 低强度脉冲超声可降低运动神经细胞的氧化应激和内质网应激。
IF 3.8 2区 物理与天体物理 Q1 ACOUSTICS Pub Date : 2024-10-28 DOI: 10.1016/j.ultras.2024.107499
Thi-Thuyet Truong , Chih-Chung Huang , Wen-Tai Chiu
Endoplasmic reticulum (ER) stress is associated with oxidative stress, which is integral to the development of various pathological conditions, including neurodegenerative disorders. In this study, using NSC-34-a hybrid cell line established by fusing motor neuron–rich embryonic spinal cord cells with mouse neuroblastoma cells-we investigated the effects of low-intensity pulsed ultrasound (LIPUS) stimulation on oxidative (reactive oxygen species)/ER stress-induced neurodegeneration. An ultrasound transducer with a center frequency of 1.15 MHz and a spatial peak temporal average intensity of 357 mW/cm2 was used for delivering ultrasound (for 8 min, via a water-filled tube) to motor neuron cells seeded in a plastic culture dish. LIPUS stimulation significantly increased the level of the antiapoptotic protein B-cell lymphoma 2 (BCL-2) and inhibited the expression of apoptosis-associated proteins such as BCL-2-associated X protein (BAX), CCAAT/enhancer-binding protein-homologous protein (CHOP), and caspase-12, thus extending the survival of motor neurons. LIPUS stimulation also enhanced Ca2+ signaling and activated the Ca2+-dependent transcription factors as nuclear factor of activated T cells (NFAT) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Furthermore, LIPUS stimulation induced the activation of the serine/threonine kinase protein kinase B (AKT). Thus, LIPUS stimulation prevented oxidative/ER stress–mediated mitochondrial dysfunction. In conclusion, as a safe and noninvasive method, LIPUS stimulation can facilitate further development of ultrasound neuromodulation as a tool for neuroscience research.
内质网(ER)应激与氧化应激有关,而氧化应激与包括神经退行性疾病在内的各种病理状况的发展密不可分。本研究利用NSC-34--一种由富含运动神经元的胚胎脊髓细胞与小鼠神经母细胞瘤细胞融合而成的杂交细胞系--研究了低强度脉冲超声(LIPUS)刺激对氧化(活性氧)/ER应激诱导的神经退行性病变的影响。使用中心频率为 1.15 MHz、空间峰值时间平均强度为 357 mW/cm2 的超声换能器向塑料培养皿中播种的运动神经元细胞发射超声波(持续 8 分钟,通过注水管)。LIPUS刺激能明显提高抗凋亡蛋白B细胞淋巴瘤2(BCL-2)的水平,抑制BCL-2相关X蛋白(BAX)、CCAAT/增强子结合蛋白同源蛋白(CHOP)和caspase-12等凋亡相关蛋白的表达,从而延长运动神经元的存活时间。LIPUS 刺激还增强了 Ca2+ 信号传导,激活了 Ca2+ 依赖性转录因子,如活化 T 细胞核因子(NFAT)和活化 B 细胞核因子卡巴轻链增强因子(NF-κB)。此外,LIPUS 还能诱导丝氨酸/苏氨酸激酶蛋白激酶 B(AKT)的活化。因此,刺激 LIPUS 可防止氧化/ER 应激介导的线粒体功能障碍。总之,作为一种安全无创的方法,LIPUS 刺激可促进超声神经调控作为神经科学研究工具的进一步发展。
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