Pub Date : 2019-10-29DOI: 10.5073/VITIS.2019.58.153-158
M. Rahali, D. Migliaro, Z. Laiadi, N. Bertazzon, E. Angelini, M. Crespan
This research focused on present grapevine biodiversity of neglected cultivars grown in 'Babar' region, Northeastern Algeria. The obtained results demonstrate the complex, rich, and even surprising inheritance of grapevine biodiversity in such a small region, with currently residual viticulture practiced only for direct consumption. Babar is one of the oldest inhabited areas in Algeria and part of the Atlas Mountains, considered very favorable for wild and cultivated vine growing since protohistoric times. Thirty-seven vines from the traditional growing area were analyzed using nuclear microsatellite (SSR) markers for cultivar identification and RT-qPCR analysis for virus detection and sanitary status evaluation. As a result, thirteen different genotypes were found, most of them showing a very good sanitary status, then constituting a valuable biological source for clonal selection. A close relatedness was evidenced with some Mediterranean varieties, resulting from previous exchanges of grapevine cultivars in the past. Furthermore, the present study highlighted the existence of three new genotypes, highly probably autochthonous of Babar region, with proposed names 'Babari', 'Babar-Algeria', and 'Amesski-Babar'. They could represent unique Algerian varieties, probably preserved over time. The conservation of these endangered genotypes is highly recommended.
{"title":"Genetic identification, origin and sanitary status of grapevine cultivars (Vitis vinifera L.) grown in Babar, Algeria","authors":"M. Rahali, D. Migliaro, Z. Laiadi, N. Bertazzon, E. Angelini, M. Crespan","doi":"10.5073/VITIS.2019.58.153-158","DOIUrl":"https://doi.org/10.5073/VITIS.2019.58.153-158","url":null,"abstract":"This research focused on present grapevine biodiversity of neglected cultivars grown in 'Babar' region, Northeastern Algeria. The obtained results demonstrate the complex, rich, and even surprising inheritance of grapevine biodiversity in such a small region, with currently residual viticulture practiced only for direct consumption. Babar is one of the oldest inhabited areas in Algeria and part of the Atlas Mountains, considered very favorable for wild and cultivated vine growing since protohistoric times. Thirty-seven vines from the traditional growing area were analyzed using nuclear microsatellite (SSR) markers for cultivar identification and RT-qPCR analysis for virus detection and sanitary status evaluation. As a result, thirteen different genotypes were found, most of them showing a very good sanitary status, then constituting a valuable biological source for clonal selection. A close relatedness was evidenced with some Mediterranean varieties, resulting from previous exchanges of grapevine cultivars in the past. Furthermore, the present study highlighted the existence of three new genotypes, highly probably autochthonous of Babar region, with proposed names 'Babari', 'Babar-Algeria', and 'Amesski-Babar'. They could represent unique Algerian varieties, probably preserved over time. The conservation of these endangered genotypes is highly recommended.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"9 1","pages":"153-158"},"PeriodicalIF":0.0,"publicationDate":"2019-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90212330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-06DOI: 10.5073/VITIS.2019.58.105-112
Á. Rodríguez‐González, P. Casquero, J. García-González, Desirée Rodríguez-Robles, J. M. M. Pozo, A. Juan-Valdés
Xylotrechus arvicola is an invasive insect on Vitis vinifera in the main wine-producing regions of the Iberian Peninsula. X. arvicola larvae bore into the grapevine wood and make galleries, which cause structural damages to the plant. The aim of this study was to investigate how grapevine wood infested by larvae affects the mechanical properties of the plant in comparation with those of uninfested wood. Samples of grapevine wood uninfested and infested by larvae were collected from vineyards. Compression and flexural strengths as well as simulated structures of grapevine wood in field, in relation to harvest weight by variety, were used to quantify the wood mechanical properties. Infested wood endured a lower strength and normal tension, and exhibited a reduction in the structural capacities in the simulation of harvest weight of 'Cabernet-Sauvignon' variety (up to 62.0 %). 'Tempranillo' (despite its high mechanical slenderness values) and 'Cabernet-Sauvignon', were the varieties that showed a higher resistance on trunks and branches, respectively. A lower bending moment was observed on the infested branches of all varieties. Changes in the mechanical properties of infested wood suggest a decrease in mechanical resistance of wood attacked by larvae that could contribute to the rupture of the infested grapevine over time. Grapevine wood attacked by X. arvicola larvae could be more sensitive to mechanical external factors in vineyards such as strong winds, harvest weight and vibration exerted by harvesting machines.
{"title":"Analysis of the mechanical properties of wood attacked by Xylotrechus arvicola (Coleoptera: Cerambycidae) larvae, and its influence on the structural properties of the plant","authors":"Á. Rodríguez‐González, P. Casquero, J. García-González, Desirée Rodríguez-Robles, J. M. M. Pozo, A. Juan-Valdés","doi":"10.5073/VITIS.2019.58.105-112","DOIUrl":"https://doi.org/10.5073/VITIS.2019.58.105-112","url":null,"abstract":"Xylotrechus arvicola is an invasive insect on Vitis vinifera in the main wine-producing regions of the Iberian Peninsula. X. arvicola larvae bore into the grapevine wood and make galleries, which cause structural damages to the plant. The aim of this study was to investigate how grapevine wood infested by larvae affects the mechanical properties of the plant in comparation with those of uninfested wood. Samples of grapevine wood uninfested and infested by larvae were collected from vineyards. Compression and flexural strengths as well as simulated structures of grapevine wood in field, in relation to harvest weight by variety, were used to quantify the wood mechanical properties. Infested wood endured a lower strength and normal tension, and exhibited a reduction in the structural capacities in the simulation of harvest weight of 'Cabernet-Sauvignon' variety (up to 62.0 %). 'Tempranillo' (despite its high mechanical slenderness values) and 'Cabernet-Sauvignon', were the varieties that showed a higher resistance on trunks and branches, respectively. A lower bending moment was observed on the infested branches of all varieties. Changes in the mechanical properties of infested wood suggest a decrease in mechanical resistance of wood attacked by larvae that could contribute to the rupture of the infested grapevine over time. Grapevine wood attacked by X. arvicola larvae could be more sensitive to mechanical external factors in vineyards such as strong winds, harvest weight and vibration exerted by harvesting machines.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"16 1","pages":"105-112"},"PeriodicalIF":0.0,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78136656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-06DOI: 10.5073/VITIS.2019.58.87-94
O. D. A. Junior, C. R. Souza, F. A. N. Dias, F. D. P. Fernandes, L. Torregrosa, C. N. Fernandes-Brum, A. C. Junior, R. V. Mota, Isabela Peregrino, M. A. D. Regina
The change of wine grape harvest from wet season (summer) to dry season (winter) by changing the pruning management has improved quality of wines produced in the Brazilian Southeast. However, the vines need to be spur pruned twice a year, i.e. with a 1st pruning in August (winter pruning) for a vegetative cycle during the hot and wet summer, and a 2nd pruning in January (summer pruning) for a productive cycle during the cold and dry season. This double pruning strategy is made necessary by the fact that latent buds developed during the dry season cycle are not fruitful to support a productive cycle in the following year. This histological study, performed in the South of Minas Gerais State (Brazil), showed that annual single pruning done in the wet season (in January) displayed a high rate of necrosis on primary and secondary buds (bud necrosis – BN). In April, 99 days after summer pruning (DASP), the rates of BN were 40 % and 50 % at basal and apical node positions, respectively, reaching 80 % of BN in December (322 DASP). As a consequence of BN, bud potential fertility was drastically reduced from 0.5 inflorescence primordial (IP) per bud (in July) to 0.06 (in December) and bud burst in the next cycle from secondary and tertiary bud axes. Vines managed by double pruning system (submitted to summer and winter pruning) displayed a much higher fruitfulness potential, i.e. 1.46 IP per bud in December (112 days after winter pruning) and limited BN occurrence (20 %). On single pruned vines, we also observed a significant decrease of starch content in canes, trunks and roots. Internal bud anatomy showed that a random cell breakdown started 70 days DASP. At 211 DASP, all buds showed a large starch granule concentration, raphides and crystals of calcium oxalate inside idioblasts of leaf primordia and also in cortical parenchyma of the vegetative axis. The bud starch content was increased and a positive correlation between necrosis and starch accumulation was observed. The impact of carbohydrate availability on bud necrosis development was discussed. This study showed that the necrosis development towards secondary and tertiary axis of the dry season buds is the main reason of unfruitfulness in the vineyards managed by single pruning in the wet season, making the double pruning compulsory.
{"title":"Effect of pruning strategy on 'Syrah' bud necrosis and fruitfulness in Brazilian subtropical Southeast","authors":"O. D. A. Junior, C. R. Souza, F. A. N. Dias, F. D. P. Fernandes, L. Torregrosa, C. N. Fernandes-Brum, A. C. Junior, R. V. Mota, Isabela Peregrino, M. A. D. Regina","doi":"10.5073/VITIS.2019.58.87-94","DOIUrl":"https://doi.org/10.5073/VITIS.2019.58.87-94","url":null,"abstract":"The change of wine grape harvest from wet season (summer) to dry season (winter) by changing the pruning management has improved quality of wines produced in the Brazilian Southeast. However, the vines need to be spur pruned twice a year, i.e. with a 1st pruning in August (winter pruning) for a vegetative cycle during the hot and wet summer, and a 2nd pruning in January (summer pruning) for a productive cycle during the cold and dry season. This double pruning strategy is made necessary by the fact that latent buds developed during the dry season cycle are not fruitful to support a productive cycle in the following year. This histological study, performed in the South of Minas Gerais State (Brazil), showed that annual single pruning done in the wet season (in January) displayed a high rate of necrosis on primary and secondary buds (bud necrosis – BN). In April, 99 days after summer pruning (DASP), the rates of BN were 40 % and 50 % at basal and apical node positions, respectively, reaching 80 % of BN in December (322 DASP). As a consequence of BN, bud potential fertility was drastically reduced from 0.5 inflorescence primordial (IP) per bud (in July) to 0.06 (in December) and bud burst in the next cycle from secondary and tertiary bud axes. Vines managed by double pruning system (submitted to summer and winter pruning) displayed a much higher fruitfulness potential, i.e. 1.46 IP per bud in December (112 days after winter pruning) and limited BN occurrence (20 %). On single pruned vines, we also observed a significant decrease of starch content in canes, trunks and roots. Internal bud anatomy showed that a random cell breakdown started 70 days DASP. At 211 DASP, all buds showed a large starch granule concentration, raphides and crystals of calcium oxalate inside idioblasts of leaf primordia and also in cortical parenchyma of the vegetative axis. The bud starch content was increased and a positive correlation between necrosis and starch accumulation was observed. The impact of carbohydrate availability on bud necrosis development was discussed. \u0000This study showed that the necrosis development towards secondary and tertiary axis of the dry season buds is the main reason of unfruitfulness in the vineyards managed by single pruning in the wet season, making the double pruning compulsory.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"23 1","pages":"87-94"},"PeriodicalIF":0.0,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82136534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-06DOI: 10.5073/VITIS.2019.58.79-86
D. Aldrich, R. Bester, J. Burger, H. J. Maree
Grapevine leafroll disease (GLD) is present in all grape-growing regions of the world and is considered the most significant grapevine viral disease. Grapevine leafroll-associated virus 3 (GLRaV-3) is considered the primary cause of GLD and in South African vineyards five genetic variant groups (I, II, III, VI and VII) have been confirmed. Biological distinctions between GLRaV-3 variants have not been fully validated. By characterising virus concentration and stress-responsive microRNA expression in GLRaV-3 infected plants, this study aimed to glean a better understanding of the possible biological distinctions between GLRaV-3 variants. Quantitative reverse transcription PCR was utilised for virus concentration ratio (VCR) determination and miRNA quantitation in GLRaV-3 positive and negative grapevines grown under greenhouse and field conditions. This study found statistically significant differences in VCRs in plants singly infected with different GLRaV-3 variants. Interestingly, no difference in mean VCRs were observed between data sets, despite notable differences in plant age, duration of GLRaV-3 infection, scion/rootstock combination and growing conditions. Several miRNAs showed statistically significant expression modulation between infected and healthy samples. miRNA expression between data sets varied substantially and a greater overall miRNA response was observed in plants with more established GLRaV-3 infections. The lack of significant differences in mean VCRs between data sets, coupled with the consistent modulation of certain miRNAs in plants that have likely been infected for longer is a promising result. This finding could indicate that successful inhibition of further virus replication by plant defence mechanisms occurred, and that these miRNAs are implicated in this response.
{"title":"Characterisation of different GLRaV-3 variant infections by determining virus concentration ratios and miRNA expression profiles","authors":"D. Aldrich, R. Bester, J. Burger, H. J. Maree","doi":"10.5073/VITIS.2019.58.79-86","DOIUrl":"https://doi.org/10.5073/VITIS.2019.58.79-86","url":null,"abstract":"Grapevine leafroll disease (GLD) is present in all grape-growing regions of the world and is considered the most significant grapevine viral disease. Grapevine leafroll-associated virus 3 (GLRaV-3) is considered the primary cause of GLD and in South African vineyards five genetic variant groups (I, II, III, VI and VII) have been confirmed. Biological distinctions between GLRaV-3 variants have not been fully validated. By characterising virus concentration and stress-responsive microRNA expression in GLRaV-3 infected plants, this study aimed to glean a better understanding of the possible biological distinctions between GLRaV-3 variants. Quantitative reverse transcription PCR was utilised for virus concentration ratio (VCR) determination and miRNA quantitation in GLRaV-3 positive and negative grapevines grown under greenhouse and field conditions. This study found statistically significant differences in VCRs in plants singly infected with different GLRaV-3 variants. Interestingly, no difference in mean VCRs were observed between data sets, despite notable differences in plant age, duration of GLRaV-3 infection, scion/rootstock combination and growing conditions. Several miRNAs showed statistically significant expression modulation between infected and healthy samples. miRNA expression between data sets varied substantially and a greater overall miRNA response was observed in plants with more established GLRaV-3 infections. The lack of significant differences in mean VCRs between data sets, coupled with the consistent modulation of certain miRNAs in plants that have likely been infected for longer is a promising result. This finding could indicate that successful inhibition of further virus replication by plant defence mechanisms occurred, and that these miRNAs are implicated in this response.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"14 1","pages":"79-86"},"PeriodicalIF":0.0,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89737620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-06DOI: 10.5073/VITIS.2019.58.95-104
Robert Rudolph, Katja Herzog, R. Töpfer, V. Steinhage
Yield and its prediction is one of the most important tasks in grapevine breeding purposes and vineyard management. Commonly, this trait is estimated manually right before harvest by extrapolation, which mostly is labor-intensive, destructive and inaccurate. In the present study an automated image-based workflow was developed for quantifying inflorescences and single flowers in unprepared field images of grapevines, i.e. no artificial background or light was applied. It is a novel approach for non-invasive, inexpensive and objective phenotyping with high-throughput. First, image regions depicting inflorescences were identified and localized. This was done by segmenting the images into the classes "inflorescence" and "non-inflorescence" using a Fully Convolutional Network (FCN). Efficient image segmentation hereby is the most challenging step regarding the small geometry and dense distribution of single flowers (several hundred single flowers per inflorescence), similar color of all plant organs in the fore- and background as well as the circumstance that only approximately 5 % of an image show inflorescences. The trained FCN achieved a mean Intersection Over Union (IOU) of 87.6 % on the test data set. Finally, single flowers were extracted from the "inflorescence"-areas using Circular Hough Transform. The flower extraction achieved a recall of 80.3 % and a precision of 70.7 % using the segmentation derived by the trained FCN model. Summarized, the presented approach is a promising strategy in order to predict yield potential automatically in the earliest stage of grapevine development which is applicable for objective monitoring and evaluations of breeding material, genetic repositories or commercial vineyards.
{"title":"Efficient identification, localization and quantification of grapevine inflorescences and flowers in unprepared field images using Fully Convolutional Networks","authors":"Robert Rudolph, Katja Herzog, R. Töpfer, V. Steinhage","doi":"10.5073/VITIS.2019.58.95-104","DOIUrl":"https://doi.org/10.5073/VITIS.2019.58.95-104","url":null,"abstract":"Yield and its prediction is one of the most important tasks in grapevine breeding purposes and vineyard management. Commonly, this trait is estimated manually right before harvest by extrapolation, which mostly is labor-intensive, destructive and inaccurate. In the present study an automated image-based workflow was developed for quantifying inflorescences and single flowers in unprepared field images of grapevines, i.e. no artificial background or light was applied. It is a novel approach for non-invasive, inexpensive and objective phenotyping with high-throughput. First, image regions depicting inflorescences were identified and localized. This was done by segmenting the images into the classes \"inflorescence\" and \"non-inflorescence\" using a Fully Convolutional Network (FCN). Efficient image segmentation hereby is the most challenging step regarding the small geometry and dense distribution of single flowers (several hundred single flowers per inflorescence), similar color of all plant organs in the fore- and background as well as the circumstance that only approximately 5 % of an image show inflorescences. The trained FCN achieved a mean Intersection Over Union (IOU) of 87.6 % on the test data set. Finally, single flowers were extracted from the \"inflorescence\"-areas using Circular Hough Transform. The flower extraction achieved a recall of 80.3 % and a precision of 70.7 % using the segmentation derived by the trained FCN model. Summarized, the presented approach is a promising strategy in order to predict yield potential automatically in the earliest stage of grapevine development which is applicable for objective monitoring and evaluations of breeding material, genetic repositories or commercial vineyards.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"20 1","pages":"95-104"},"PeriodicalIF":0.0,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88653037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-06DOI: 10.5073/VITIS.2019.58.113-122
Eric Vukicevich, T. Lowery, M. Hart
Although the use of under-trellis plants as weed control (living mulch) in vineyards has been gaining popularity, its effects on soil quality and especially soil biology have not been well studied. Due to functional trait differences, plants may differ in how they compete with vines, and may also change abiotic and biotic soil properties. A living mulch trial was established in the semi-arid Okanagan valley of British Columbia comparing vine growth as well as soil abiotic and biotic outcomes for four living mulch treatments: buffalo grass (Bouteloua dactyloides), Chewing's fescue (Festuca rubra ssp. commutata), birdsfoot trefoil (Lotus corniculatus), and shepherd's purse (Capsella bursa-pastoris) with two industry standards: herbicide and cultivation. After two seasons, strong vine growth responses were seen that depended on living mulch identity, e.g., reduction in leaf N status with grasses, reduction in leaf water potential with the legume, birdsfoot trefoil. These effects were related to plant-induced changes to soil C:N ratio and soil moisture. Although treatments did not change abundance of the measured fungal guilds in bulk soil, abundance of arbuscular mycorrhizal fungi in vine roots was lowest with birdsfoot trefoil as living mulch. This study may help growers to select living mulch species appropriate for the soil conditions and resource availability of their site.
{"title":"Effects of living mulch on young vine growth and soil in a semi-arid vineyard","authors":"Eric Vukicevich, T. Lowery, M. Hart","doi":"10.5073/VITIS.2019.58.113-122","DOIUrl":"https://doi.org/10.5073/VITIS.2019.58.113-122","url":null,"abstract":"Although the use of under-trellis plants as weed control (living mulch) in vineyards has been gaining popularity, its effects on soil quality and especially soil biology have not been well studied. Due to functional trait differences, plants may differ in how they compete with vines, and may also change abiotic and biotic soil properties. A living mulch trial was established in the semi-arid Okanagan valley of British Columbia comparing vine growth as well as soil abiotic and biotic outcomes for four living mulch treatments: buffalo grass (Bouteloua dactyloides), Chewing's fescue (Festuca rubra ssp. commutata), birdsfoot trefoil (Lotus corniculatus), and shepherd's purse (Capsella bursa-pastoris) with two industry standards: herbicide and cultivation. After two seasons, strong vine growth responses were seen that depended on living mulch identity, e.g., reduction in leaf N status with grasses, reduction in leaf water potential with the legume, birdsfoot trefoil. These effects were related to plant-induced changes to soil C:N ratio and soil moisture. Although treatments did not change abundance of the measured fungal guilds in bulk soil, abundance of arbuscular mycorrhizal fungi in vine roots was lowest with birdsfoot trefoil as living mulch. This study may help growers to select living mulch species appropriate for the soil conditions and resource availability of their site.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"42 1","pages":"113-122"},"PeriodicalIF":0.0,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75307899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-13DOI: 10.5073/VITIS.2019.58.71-78
J. C. Bettoni, R. Bonnart, A. Shepherd, A. Kretzschmar, G. Volk
Many genebanks rely on cryopreservation as a method to preserve vulnerable field collections of vegetatively propagated crops. Effective cryopreservation procedures have been identified for Vitis; however, they usually use in vitro plantlets as the shoot tip source materials. It is costly to establish Vitis collections in vitro prior to cryopreservation. We sought to determine if growth chamber derived Vitis plants could serve as the source of shoot tips for cryopreservation. Nodal sections from growth chamber derived plants were surface-disinfected and placed in tissue culture on pre-treatment medium for 2 weeks. Uniform apical shoot tips (1 mm) were first obtained from the nodal sections and then precultured for 3 days on medium containing 0.3 M sucrose, salicylic acid, glutathione (reduced form), ascorbic acid and plant preservative mixture. Half-strength PVS2 was applied for 30 min at 22 °C, prior to full-strength PVS2 treatment at 0 °C. Cryopreserved shoot tips had the highest average regrowth of 50 and 55 % without and with cold-acclimation followed with a full-strength PVS2 exposure duration of 40 and 30 min at 0 °C, respectively. This cryopreservation protocol achieved high percentages of regrowth in V. vinifera 'Chardonnay' and 'Riesling' and V. hybrid 'Oppenheim'. Histological observations revealed that shoot tips from growth chamber plants had apical as well as multiple lateral meristems that survived LN immersion. The preservation of multiple meristems in each shoot tip may increase the capacity of shoot tip regeneration in cryopreserved Vitis that originates from ex vitro sources. The high percentage of regrowth after shoot tip cryopreservation using Vitis shoot tips derived from growth chamber source plants suggest that it may be possible to cryopreserve Vitis shoot tips without first introducing each accession into tissue culture.
{"title":"Cryopreservation of grapevine (Vitis spp.) shoot tips from growth chamber-sourced plants and histological observations","authors":"J. C. Bettoni, R. Bonnart, A. Shepherd, A. Kretzschmar, G. Volk","doi":"10.5073/VITIS.2019.58.71-78","DOIUrl":"https://doi.org/10.5073/VITIS.2019.58.71-78","url":null,"abstract":"Many genebanks rely on cryopreservation as a method to preserve vulnerable field collections of vegetatively propagated crops. Effective cryopreservation procedures have been identified for Vitis; however, they usually use in vitro plantlets as the shoot tip source materials. It is costly to establish Vitis collections in vitro prior to cryopreservation. We sought to determine if growth chamber derived Vitis plants could serve as the source of shoot tips for cryopreservation. Nodal sections from growth chamber derived plants were surface-disinfected and placed in tissue culture on pre-treatment medium for 2 weeks. Uniform apical shoot tips (1 mm) were first obtained from the nodal sections and then precultured for 3 days on medium containing 0.3 M sucrose, salicylic acid, glutathione (reduced form), ascorbic acid and plant preservative mixture. Half-strength PVS2 was applied for 30 min at 22 °C, prior to full-strength PVS2 treatment at 0 °C. Cryopreserved shoot tips had the highest average regrowth of 50 and 55 % without and with cold-acclimation followed with a full-strength PVS2 exposure duration of 40 and 30 min at 0 °C, respectively. This cryopreservation protocol achieved high percentages of regrowth in V. vinifera 'Chardonnay' and 'Riesling' and V. hybrid 'Oppenheim'. Histological observations revealed that shoot tips from growth chamber plants had apical as well as multiple lateral meristems that survived LN immersion. The preservation of multiple meristems in each shoot tip may increase the capacity of shoot tip regeneration in cryopreserved Vitis that originates from ex vitro sources. The high percentage of regrowth after shoot tip cryopreservation using Vitis shoot tips derived from growth chamber source plants suggest that it may be possible to cryopreserve Vitis shoot tips without first introducing each accession into tissue culture.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"84 1","pages":"71-78"},"PeriodicalIF":0.0,"publicationDate":"2019-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80572690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-13DOI: 10.5073/VITIS.2019.58.53-57
C. Ocete, R. A. García, G. Lovicu, Álvaro Rodríguez-Miranda, J. Melón, M. Cantos, J. L. Fernández, M. Lara, F. Canales, J. Llompart, E. Rodríguez, C. Weiland, R. Rubio
This paper is focused on 21 relic wild grapevine populations within Huelva province (SW Europe, Spain) prospected between 2015-2017. Position of each population along river-bank forests, types of flower of this dioecious subspecies and morphology of the pollen grains were described. Analyses of five microvinifications were carried out, which indicate that the color intensity of the wines is between 14.6 and 17.6 and the pH between 3.26-3.27, which are suitable values for red wines under Mediterranean climatology. A list of the accompanying vegetation and the cultivated varieties in the "Condado de Huelva" Registered Appellation Origin Mark were also included. Moreover, the genetic diversity of 23 vines was characterized using 25 nuclear SSR loci, the results showed a slightly higher diversity than the one found in Iberian cultivars. However, the observed heterozygosity was significantly lower than the expected one for wild populations in the Huelva province. The inbreeding depression in these wild grape populations is suggested by the positive F values. Therefore, the conservation of this unique germplasm collection should be part of the process of maintaining the genetic diversity in this gene pool, especially, taking into account that no particular legal figure of preservation exists in Spain.
本文以2015-2017年西班牙西南部韦尔瓦省(Huelva) 21个野生葡萄种群为研究对象。描述了各居群沿河岸林的分布位置、该雌雄异株亚种的花型和花粉粒形态。对5种微发酵条件进行了分析，结果表明，葡萄酒的颜色强度在14.6 ~ 17.6之间，pH值在3.26 ~ 3.27之间，适合地中海气候条件下的红葡萄酒。同时还列出了“Condado de Huelva”注册名称原产地标志的配套植被和栽培品种清单。利用25个SSR位点对23个品种的遗传多样性进行了分析，结果表明，该品种的遗传多样性略高于伊比利亚品种。然而，观察到的杂合度明显低于韦尔瓦省野生种群的预期杂合度。这些野生葡萄群体的近交抑制表现为正F值。因此，保护这一独特的种质资源应该是维护该基因库遗传多样性过程的一部分，特别是考虑到西班牙没有特定的法律保护数字。
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Pub Date : 2019-01-28DOI: 10.5073/VITIS.2019.58.23-31
T. Tian, S. Gu
In warmer regions, forcing vines to regrow and shifting fruit ripening to the cooler portion of the growing season can increase the concentration of total fruit anthocyanins (TFA) in red winegrapes, but the effect on anthocyanin composition remains unclear. Additionally, irrigation reduction post veraison was reported to improve fruit anthocyanins in cool and temperate regions with low precipitation, whereas this response has not been previously examined in forced vines grown in dry and warm regions. Experiments were conducted with 'Cabernet Sauvignon' (Vitis vinifera L.) over two consecutive years in Fresno, California, to investigate the effect of shifting fruit ripening on the concentration and composition of fruit anthocyanins as well as the influence of post-veraison irrigation reduction on fruit anthocyanins of forced vines. Vines under conventional practices (non-forced) were used as the control. Forcing treatment included removing primary leaves, clusters, and laterals, as well as hedging primary shoots, in mid-June. Control vines were irrigated at 80 % crop evapotranspiration (ETC) post veraison, whereas forced vines were irrigated at 40, 60, 80, or 100 % ETC post veraison. Results suggest that forcing vines to regrow and shifting fruit ripening led to a significant increase of TFA, primarily non-acylated anthocyanins, during fruit ripening and at harvest over two years. Forcing treatment also altered composition of fruit anthocyanins at harvest, with increased proportions of TFA comprised by the glucosides of delphinidin and petunidin but the decreased proportion of the glucosides of malvidin. This study demonstrates that forcing vines to regrow and shifting fruit ripening in the warmer region can lead to a more balanced profile of fruit anthocyanins, with improved non-acylated derivatives and altered relative abundance of the glucosides of five anthocyanidins. Reducing irrigation post veraison, however, had only a minor effect on fruit anthocyanins in forced vines.
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