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One of the most pressing medical, social and government tasks is to ensure health saving, improve the quality of life and increase the life expectancy of the Russian population. The most important lever to ensure the solution of these issues is the optimization of nutrition and the use of methods of artificial intelligence (AI) and new information technologies to support decision-making in this area. The purpose of the study was to familiarize specialists with the Scientific Instrument for Nutrition Analysis (SINA), created on the basis of AI technologies to optimize nutrition and prevent alimentary diseases in order to preserve the health of the population of the Russian Federation. Results. A patent was created and received for a scientific web tool (SINA) based on AI technologies for actual nutrition analyzing with subs equent assessment of health status and generation of recommendations, including a personalized diet, which is formed based on individual human needs for energy, nutrients and bioactive compounds, taking into account genome, gender characteristics, age, food preferences, profession, diseases, medication treatment, clinical blood parameters, individual morphological and constitutional characteristics, his physical activity, etc. Conclusion. The results of using SINA can reduce the labor costs of doctors; personalize diets as much as possible taking into account many factors; increase patient adherence to the prescribed diet and recommendations; increase public awareness of nutrition issues, reduce morbidity rates and improve public health. The high-tech approach using AI is advanced and is capable of solving many key challenges in the field of health care, prevention and medicine in general, and improve the quality of life of the population.

The problem of the increasing obesity among children and adolescents is urgent. One of the most interesting and promising directions in this area is to study the correlation of individual microorganisms with the presence and absence of obesity. The aim of the study was to assess the correlation between the isolation frequency of individual microorganisms and the presence of obesity in children and adolescents and to identify possible associations between different groups of microorganisms in obese patients. Material and methods. 156 male and female patients aged from 7 to 17 years were included in the study. The patients were divided into a control group (n=23) (healthy patients), a group of children with exogenous constitutional obesity without complications (n=25), a group of children who had one or more complications of obesity (n=108). For all patients body mass index (BMI) was calculated. Additional examination included a cultural study of the intestinal microbiota. Fecal samples of patients were used as the material. Preparation of the material for inoculation, inoculation and subsequent incubation of the Petri plates were carried out under anaerobic conditions. The isolated microorganisms were identified using the MALDI-ToF mass spectrometry method. Results. When analyzing the correlation between obesity and individual taxa, statistically significant differences were obtained only for Bifidobacterium spp. (p=0.045). The analysis of the correlation between obesity and the isolation of individual microorganisms has shown that Bifidobacterium pseudocatenulatum (p=0.012), Candida albicans (p=0.012), Streptococcus salivarius (p=0.016), Bifidobacterium breve (p=0.003), Veillonella parvula (p=0.013), Haemophilus parainfluenzae (p=0.003), Streptococcus oralis (p=0.001), Weissella confusa (p=0.036), Enterococcus mundtii (p=0.036) were isolated less often in patients with obesity than in control group. Conclusion. The results of the study has demonstrated that only one taxon, Bifidobacterium spp., had a significant correlation with the absence of obesity. At the same time, a reliable correlation with the absence of obesity was also established for individual microorganisms, including several microorganisms from Bifidobacterium spp. and Streptococcus spp., which may enable to establish certain microbiological predictors of obesity and its complications.

The study of the genetic determinants of the disaccharidase activity opens up new prospects for improving diagnostics and choosing medical tactics in gastroenterology. The aim of the study was to systematize the data on the role of the sucrase-isomaltase gene (SI) in regulating sucrose metabolism and the contribution of SI mutations to the prevalence of sucrose malabsorption disorders (sucrase-isomaltase deficiency, SID) and certain forms of enterological pathology in different population groups. Material and methods. A review of the peer-reviewed scientific literature, mainly in the PubMed database (https://pubmed.ncbi.nlm.nih.gov) and eLibrary (https://elibrary.ru), was conducted using key words: carbohydrate malabsorption, sucrase, sucrase-isomaltase deficiency, sucrase-isomaltase SI gene. The search depth was not specified, but particular attention was paid to recent publications. The gnomAD database (https://www.ncbi.nlm. nih.gov/snp/rs781470490) was also used. Results. According to the review results, 37 out of 150 known SI gene mutations have been confirmed to contribute to reduced sucrase activity or restricted sucrase production. The prevalence of point mutations in the SI gene is estimated at 0.0006%, but carrier rates of the SI delAG deletion (rs781470490), manifested as homozygosity in SID, are very high (5-21%) in indigenous populations of Arctic regions in East Asia and America. Medicalgenetic research methods improve the accuracy of differential diagnosis of primary and secondary SID and other forms of disaccharide and polysaccharide malabsorption. The formation of databases on the prevalence of genetic determinants of sucrase-isomaltase insufficiency is a promising way to refine the epidemiology of SID. There is an increased (0.2-2.3%) risk of clinical manifestations of SID in homozygous carriers of the SI delAG mutation in the Chukotka, Kamchatka, and Northern Priochotye populations. Verification of reports on a less pronounced tendency to lipid metabolism disorders in SI delAG carriers compared with the control group is recommended. Conclusion. Manifestations of mutant SI variants in the phenotype are associated with the presence of accompanying carbohydrate malabsorption variants and specific gut microbiota. The SI 15Phe variant (rs9290264) may contribute to the development of irritable bowel syndrome.

The improvement of the novel foods' safety assessment algorithms is currently one of the food hygiene significant areas. Within the studying of Hermetia illucens insects' effect, the standard in vivo allergological research integrated in the protocol of medical and biological evaluation of genetically modified food has been used. The protocol was supplemented with cytokine profile indicators and pathomorphologic characteristics of immunocompetent organs' lymphoid tissue. The purpose of the research was to study the effect of black soldier fly (Hermetia illucens) larvae biomass on the rats' immune status in the experiment on the induced anaphylactic shock model. Material and methods. The effect of black soldier fly (Hermetia illucens) larvae biomass was studied in a 29-day experiment on growing (43-72 days of life) male Wistar rats fed with Hermetia illucens biomass - main group (n=29) and semi-synthetic casein diet - control group (n=29). The complex assessment of allergenic potential of Hermetia illucens biomass was carried out in the experiment on the induced anaphylactic shock model in Wistar rats. An expanded pool of immune status indicators was studied including active anaphylactic shock severity (lethality, number of severe anaphylaxis reactions, anaphylactic index); cytokine profile (content of proinflammatory and anti-inflammatory cytokines, as well as regulators of cellular and humoral immune response); IgG1 and IgG4 level before and after administration of ovalbumin permissive dose (4 mg/kg b.w.). In addition to this pathomorphologic characteristics of lymphoid tissue of the main immunocompetent organs (thymus, spleen, Payer's patches) have been obtained. Results. The significant systemic anaphylaxis reaction decrease in the main group has been shown. Comparative assessment of the serum cytokines (GM-CSF, IFN-γ, IL-10, IL-12(p70), IL-13, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, TNF-α) as well as the level of immunoglobulins of the IgG1, IgG4 class before and after administration of ovalbumin permissive dose did not reveal significant differences in rats of the control and main groups. In the main group, there was a decrease in blood serum proallergic cytokines: the level of IL-4 reduced by 1.3 fold, IL-10 - 1.1 and IL-13 - 1.2 fold (p>0.05), and in animals with mild anaphylactic reaction - by 1.8, 1.4 and 1.4 times, respectively (p>0.05). The morphologic studies of the immune system organs showed no intergroup differences. Conclusion. Thus, allergological studies of black soldier fly (Hermetia illucens) larvae in the experiment with the use of systemic anaphylaxis rat model and determination of immune status indicators (anaphylactic shock severity, cytokine profile, IgG1 and IgG4 level, morphologic structure of immunocompetent organs) did not reveal any allergenic effect of the studied product.

Short-chain fatty acids (SCFAs) are an important link in the maintenance and normalization of some important body functions. Recently, the metabolic component of the SCFAs effects has also been actively studied; the effect on body weight, insulin resistance and glycemia is of particular interest in the context of the prevention and treatment of carbohydrate metabolism disorders. In this regard, it is relevant to study the characteristics of SCFAs' production in patients with impaired carbohydrate metabolism, mainly with type 2 diabetes mellitus (T2DM). The purpose of the research was to study the modern data on the synthesis peculiarities of SCFAs in healthy people and patients with impaired carbohydrate metabolism. Material and methods. The data of domestic and foreign literature presented in PubMed, Google Scholar, ResearchGate, Elsevier, eLibrary, CyberLeninka databases, published mainly over the past 10 years, have been analyzed. Results. According to the concept of the philometabolic nucleus, bacteria of certain species, which are of the greatest importance compared to the rest, are responsible for the synthesis of specific SCFAs. The gut microbiota (GM) has the property of plasticity - the ability to change its composition under the influence of various factors. Most studies describe the effect of GM and its metabolites on the carbohydrate metabolism, but an equally important aspect of this process is the effect of carbohydrate metabolism disorders on GM and its functional activity. In case of disorders of carbohydrate metabolism, some altered components of homeostasis negatively affect GM and its production of SCFAs. As a result, the total amount and variety of SCFAs decrease, which exacerbate the imbalance in relation to carbohydrate metabolism. There is evidence that in patients with T2DM, the concentration of butyrate, which has a positive effect on insulin resistance, body weight, fasting glycemia and postprandial glycemia, decreases. The concentration of propionate and acetate, which didn't show such a pronounced positive effect in studies on carbohydrate metabolism, on the contrary, increases. Conclusion. The production of SCFAs by GM representatives depends on many factors, such as nutrition, physical activity, medication intake and the presence of chronic diseases. Numerous studies have confirmed the difference in the characteristics of the production of SCFAs in patients with T2DM and healthy people. The study of the peculiarities of GM metabolism in patients with T2DM is a tool in understanding the basics of therapy and lifestyle correction in both patients with T2DM and healthy people in order to prevent disorders of carbohydrate metabolism.

The excessive consumption of sugar-containing foods contributes to the development of a number of diseases, including obesity, diabetes mellitus, etc. As a substitute for sugar, people with diabetes mellitus and obesity most often use sweeteners. Sweet proteins, in particular brazzein, are an alternative to synthetic sweeteners that have natural origin, are broken down in the intestines along with food proteins, and do not affect blood sugar and insulin levels. The purpose of the review was to analyze the available data on the sweet protein brazzein, its physical and chemical properties, existing biotechnological methods of production, and prospects for application in the food industry in order to further develop an optimized heterologous expression system. Material and methods. Google Scholar, Scopus, Web of Science, PubMed, RSCI and eLibrary.ru databases were used for collecting and analyzing literature. Search depth - 30 years. Results. Numerous studies of the physical and chemical properties of brazzein have demonstrated its high potential for use in the food industry. In particular, a short amino acid sequence, thermal stability, the ability to maintain its structure and sweet properties in a wide pH range, hypoallergenicity, lack of genotoxicity, and an extremely high level of sweetness compared to sucrose allow us to conclude that its use is promising. Mutant variants of brazzein have been generated, the sweetest of which (with three amino acid substitutions H31R/E36D/E41A) exceeds sucrose sweetness by 22 500 times. To date, various systems for the expression of recombinant brazzein have already been developed, in which bacteria (Escherichia coli, Lactococcus lactis, Bacillus licheniformis), yeast (Komagataella phaffii, Kluyveromyces lactis, Saccharomyces cerevisiae), plants (Zea mays, Oryza sativa, Lactuca sativa, Nicotiana tabacum, Daucus carota) and animals (Mus musculus) have been used. Conclusion. Due to its high sweetness, organoleptic properties and long history of human consumption, brazzein can be considered as a promising natural sweetener. Despite the short peptide sequence, the production of the recombinant protein faced a number of problems, including low protein yield (for example, it could only be detected in mouse milk by Western blot hybridization) and loss of sweetness. Thus, further optimization of the process is necessary for widespread brazzein use in the food industry, which includes the selection of an adequate producer and the use of extracellular expression systems to reduce the final cost of the product.

Retrospective analysis of medical and organizational problems of food supply in besieged Leningrad is important for the development of the principles of population nutrition in extreme situations. The aim of the paper was to characterize the work of dairy kitchens that provided nutrition to infants in Leningrad during the blockade years (1941-1944) on the basis of documentary data. Material and methods. The study was prepared on the materials of the archives of St. Petersburg, considered from the position of the social history of medicine, which is centered on the study of medical practice. Results. In the blockaded Leningrad under the conditions of food, electricity, and water supply shortages, there was a forced abandonment of a number of principles of infant feeding generally recognized by the pediatricians. Almost all infants born in the blockade ring were transferred to artificial feeding with the release of infant formula through milk kitchens. In conditions of food shortages, children under the age of three were also provided with centralized milk products. The activities of the staff of the milk kitchens were carried out in close contact with doctors of regional children's consultations and specialists of the Leningrad Pediatric Institute, who developed issues of rational nutrition for infants. During the blockade years, the formulae developed before the war were generally retained, but due to the lack of dairy resources, vegetable products were used as substitutes. As the food situation in the city improved, doctors and milk kitchens reverted to pre-war recipes, especially for infant nutrition. The products of the milk kitchens were subject to constant supervision by sanitary physicians. Laboratory tests indicated that, in terms of basic microbiological indicators, infant formulae were generally safe and differed slightly from those produced before the war. Conclusion. In blockaded Leningrad (especially during the winter of 1941/1942), the products of dairy kitchens served, as a rule, as the only source of nutrition for infants. Despite a number of organizational difficulties and abuse of workers, the preservation of the network of milk kitchens, centralized supply of milk and formula through them, and expansion of the contingents attached to them justified themselves.

Human exposure to microplastics contained in food has become a serious problem due to the growing accumulation of microplastics in a wide range of different ecosystems: from terrestrial to aquatic, in the organs of various organisms, including humans. The aim of this research was to summarize data on microplastic contamination of mass consumption products such as bottled drinking water, beverages, including sweet carbonated, dairy products, fish and other seafood. Material and methods. The search for studies and literature reviews on the topic of studying contamination of food and beverages with plastic microparticles was carried out using the databases PubMed, Science Daily, ResearchGate by keywords: microplastics, food contamination, the effect of microplastics on the human body. 72 articles by foreign authors have been studied. Results. The review provides up-to-date information on microplastic contamination of mass-consumed food in the world: drinking water, carbonated drinks, beer, milk. Most studies of microplastic contamination focus on marine and freshwater food sources. Although there is a large amount of research on seafood, such as fish and shellfish, it is difficult to assess the overall effects of microplastics on humans in general when consuming food due to the lack of convincing data on many other foods. Microplastics can be added or removed from food raw materials during processing and cooking. Conclusion. Consumption of food contaminated with microplastics poses a potential risk to human health. Studies of the microplastic intake with food, drinking water and beverages, as well as their distribution in the body, should be a priority to understand their impact and potential risk to human health. Research on processed foods is crucial to assess the contribution of food to the overall human consumption of microplastics and to assess the risks of exposure.

Obesity is a complex chronic disease with multifactorial etiology. The global obesity epidemic and the exponential rate of increase in its prevalence among the population represent a significant burden on human health being a serious public healthcare problem. The aim of the review was to analyze available literature on obesity and key risk predictors. Material and methods. A literature search was conducted for the last 10 years using the library platforms PubMed, Elsevier, eLIBRARY by keywords: obesity; genes; polymorphism; predisposition; genomic associations; risk factors; lifestyle; nutrition). Results. Studies have shown that lifestyle and heredity are considered as reliably strong predictors of the obesity risk. Suboptimal nutrition and eating disorders currently reported at both population and individual levels model the effects associated with the risk of metabolic disorders, including dyslipidemia, hyperinsulinemia, hyperglycemia, hypertension, hyperuricemia, etc. It should be noted that body fat percentage is directly related to human cardiometabolic state, which suggests a new type - obesity with normal body weight. Of concern is the fact that, due to normal body weight, this category of persons remains out of sight of healthcare and, accordingly, appropriate preventive measures will not be taken. With regard to genetic risk factors for obesity, in some patients, gene mutations may not manifest themselves throughout life, and when detected, they may not always be the cause of obesity, being the result of individual variability. Recent decades have been characterized by the popularity of «nudge theory,» but the need for additional research to determine optimal combinations and contexts to encourage the right choice has been noted. The ambiguity of the results obtained is due to the fact that in modern society an environment has been created that is set up quite aggressively to the principles of optimal nutrition. Conclusion. The data obtained are needed to develop new strategies for preventive measures to reduce the prevalence of obesity, promote population health and increase the period of active longevity.