Wellcome Open Research最新文献

We present a genome assembly from a specimen of Phakellia ventilabrum (Porifera; Demospongiae; Bubarida; Bubaridae). The genome sequence has a total length of 211.92 megabases. Most of the assembly (99.97%) is scaffolded into 25 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 24.36 kilobases in length. Gene annotation of this assembly by Ensembl identified 21 622 protein-coding genes. Thirty-three binned genomes were generated from the metagenome assembly, of which eight were classified as high-quality metagenome assembled genomes (MAGs) and of which four of the MAGs are fully circular. The MAGs were taxonomically assigned to Pseudomonadota (i.e. Candidatus Poriferihabitaceae), Nitrospirota, Nitrospinota, and the archaeal Nitrosopumilus clade.

We present a genome assembly from an individual male Kyklioacalles roboris (cryptorhynchine weevil; Arthropoda; Insecta; Coleoptera; Curculionidae). The assembly contains two haplotypes with total lengths of 698.93 megabases and 609.02 megabases. Most of haplotype 1 (87.44%) is scaffolded into 20 chromosomal pseudomolecules, including the X and Y sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 20.21 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

We present a genome assembly from an individual male Malthodes minimus (soldier beetle; Arthropoda; Insecta; Coleoptera; Cantharidae). The genome sequence has a total length of 583.60 megabases. Most of the assembly (97.75%) is scaffolded into 7 chromosomal pseudomolecules, including the X and Y sex chromosomes. The mitochondrial genome has also been assembled, with a length of 19.48 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

We present a genome assembly from an individual female Hylaeus dilatatus (Chalk Yellow-face Bee; Arthropoda; Insecta; Hymenoptera; Colletidae). The assembly contains two haplotypes with total lengths of 307.38 megabases and 314.32 megabases. Most of haplotype 1 (82.9%) is scaffolded into 12 chromosomal pseudomolecules. Most of haplotype 2 (73.52%) is scaffolded into 12 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 17.78 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

We present a genome assembly from an individual female Mesoplodon mirus (True's beaked whale; Chordata; Mammalia; Artiodactyla; Ziphiidae). The assembly contains two haplotypes with total lengths of 3 442.40 megabases and 2 956.53 megabases. Most of haplotype 1 (79.62%) is scaffolded into 21 chromosomal pseudomolecules, including the X sex chromosome. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 16.35 kilobases. Gene annotation of this assembly on Ensembl identified 18 422 protein-coding genes. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

We present a genome assembly from an individual female Arthaldeus pascuellus (leafhopper; Arthropoda; Insecta; Hemiptera; Cicadellidae). The genome sequence has a total length of 1 275.44 megabases. Most of the assembly (99.4%) is scaffolded into 9 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled, with a length of 20.4 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

We present a genome assembly from an individual male Balaenoptera physalus (fin whale; Chordata; Mammalia; Artiodactyla; Balaenopteridae). The assembly contains two haplotypes with total lengths of 3 442.54 megabases and 2 850.21 megabases. Most of haplotype 1 (79.11%) is scaffolded into 23 chromosomal pseudomolecules, including the X and Y sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 16.4 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

Background: We investigated the main drivers for current shortages of licensed antibiotics, existing mitigation strategies undertaken and potential barriers in their implementation through in-depth interviews with experts operating in select regions.

Methods: Purposive sampling was used to identify opinion leaders with expertise in managing antibiotic supply chains, access, and shortages between August 2023 and April 2024. Consented participants were interviewed using a semi-structured interview guide developed using the PESTELI (political, economic, sociological, ecological, technological, legal, industry) framework. Data collection and analysis were iterative and recursive, using constant comparison.

Results: Participants who were interviewed (n=16) had local, national, and global roles in managing and studying access, supply, and demand chain management from Europe, South Africa, India and USA. Political engagement on antibiotic shortages is reported to facilitate effective mitigation strategies, especially in areas where there is strong evidence of government investment. Legal measures have also been used; for example, pharmacists in the UK being given rights to automatically substitute antibiotics on prescriptions and negotiating with pharmaceutical companies for greater transparency on the challenges in manufacturing. Economic incentives are currently missing and are recognised as being a driver for lack of engagement on this topic from pharmaceutical industry. Greater transparency is required from the pharmaceutical companies on the manufacturing chain issues that may lead to shortages. Technologically there is a major gap in systems to forecast and manage antibiotic shortages. Sociological elements include adopting appropriate communication to not cause panic buying and hoarding by organisations when there is an impending shortage. Legislative changes are linked to the political and economic barriers for cohesive systems to manage the antibiotic manufacture and supply chain in relation to shortages.

Conclusion: Currently there are limited interventions to respond to and manage shortages. The antibiotic manufacture and supply chain is complex and under the influence of PESTELI indicators which will need to be understood and mitigated in different contexts and regions.

We present a genome assembly from an individual male Colocasia coryli (Nut-tree Tussock; Arthropoda; Insecta; Lepidoptera; Noctuidae). The genome sequence has a total length of 768.61 megabases. Most of the assembly (99.73%) is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled, with a length of 15.31 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

We present a genome assembly from an individual male Colostygia multistrigaria (Mottled Grey; Arthropoda; Insecta; Lepidoptera; Geometridae). The assembly contains two haplotypes with total lengths of 482.72 megabases and 504.67 megabases. Most of haplotype 1 (99.19%) is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 17.63 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.