Wellcome Open Research最新文献

We present a genome assembly from an individual female Araneus angulatus (orb-weaver spider; Arthropoda; Arachnida; Araneae; Araneidae). The assembly contains two haplotypes with total lengths of 2 980.91 megabases and 2 941.08 megabases. Most of haplotype 1 (94.74%) is scaffolded into 28 chromosomal pseudomolecules. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 14.53 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

We present a genome assembly from an individual female Anthomyza gracilis (anthomyzid fly; Arthropoda; Insecta; Diptera; Anthomyzidae). The assembly contains two haplotypes with total lengths of 576.40 megabases and 595.67 megabases. Most of haplotype 1 (97.03%) is scaffolded into 6 chromosomal pseudomolecules. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 16.87 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

We present a genome assembly from an individual male Cataclysta lemnata (Small China-mark; Arthropoda; Insecta; Lepidoptera; Crambidae). The genome sequence has a total length of 432.44 megabases. Most of the assembly (99.51%) is scaffolded into 28 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled, with a length of 15.36 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

We present a genome assembly from an individual female Chrysops viduatus (Square-spot Deerfly; Arthropoda; Insecta; Diptera; Tabanidae). The genome sequence has a total length of 312.35 megabases. Most of the assembly (87.6%) is scaffolded into 5 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 16.08 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

We present a genome assembly from an individual male Phragmataecia castaneae (Reed Leopard moth; Arthropoda; Insecta; Lepidoptera; Cossidae). The assembly contains two haplotypes with total lengths of 590.72 megabases and 591.65 megabases. Most of haplotype 1 (99.8%) is scaffolded into 30 chromosomal pseudomolecules, including the Z sex chromosome. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.5 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

We present a genome assembly from an individual female Oxystoma pomonae (seed weevil; Arthropoda; Insecta; Coleoptera; Apionidae). The genome sequence has a total length of 1 174.34 megabases. Most of the assembly (99.97%) is scaffolded into 11 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled, with a length of 18.2 kilobases. Gene annotation of this assembly on Ensembl identified 14 352 protein-coding genes. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

We present a genome assembly from an individual male Aphidecta obliterata (larch ladybird beetle; Arthropoda; Insecta; Coleoptera; Coccinellidae). The genome sequence has a total length of 349.56 megabases. Most of the assembly (97.85%) is scaffolded into 10 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled, with a length of 17.95 kilobases. Gene annotation of this assembly on Ensembl identified 18 868 protein-coding genes. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

We present the scaffold-level genome assemblies of four Danio species, released in 2020: Danio albolineatus, Danio choprai, Danio jaintianensis and Danio tinwini (Chordata; Actinopterygii; Cypriniformes; Cyprinidae). Genome sizes range from 1,102.16 Mb for Danio choprai to 1,497.36 Mb for Danio tinwini.

We present a genome assembly from an individual female Hypomecis atomaria (Common Heath; Arthropoda; Insecta; Lepidoptera; Geometridae). The genome sequence has a total length of 690.01 megabases. Most of the assembly (99.95%) is scaffolded into 32 chromosomal pseudomolecules, including the W and Z sex chromosomes. The mitochondrial genome has also been assembled, with a length of 15.6 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

Background: Extreme heat exposure - defined as sustained ambient temperatures exceeding local thresholds - has been associated with several adverse pregnancy outcomes, including preterm birth, stillbirth, gestational diabetes and small for gestational age. However, the mechanisms linking environmental heat to these outcomes, and the biological markers that signify individual vulnerability, are not well understood. We present the protocol for a prospective cohort study within the Heat in Pregnancy-India consortium (HiP-India). This study aims to characterise the physiological and pathophysiological responses of the mother, placenta, and fetus to varying levels of temperature, humidity, and air pollution exposure, and to identify the critical windows and mechanisms of heat-related risk during pregnancy.

Methods: 600 women with singleton pregnancies, with confirmed gestational age by ultrasound between 11-14 weeks, will be recruited prospectively from three distinct climate zones in India: Gurugram, Delhi NCR 'semi-arid'; Bilaspur, Chhattisgarh 'humid sub-tropical and tropical wet and dry'; and Puducherry 'tropical wet and dry'. Each participant will have their level of exposure to heat, humidity and air pollution measured for 24 hours each trimester in their home and/or workplace using individual and area monitoring devices. Perceived heat stress will be captured using a modified HOTHAPS questionnaire, while physiological heat strain will be measured through urinary specific gravity, core body temperature, heart rate and blood pressure. Within 48 hours of environmental monitoring, maternal haemodynamic parameters will be assessed non-invasively. Fetal ultrasound will be performed to evaluate growth and fetal-placental blood flow, and maternal blood samples collected to evaluate circulating biomarkers of placental function and stress. Cardiotocography will be conducted in the third trimester only. Delivery outcomes for both mothers and neonates will be extracted from hospital records and interviews. In a subset of 100 women, markers of lactation physiology will be recorded during the first 2 weeks after delivery.

Ethics and dissemination of results: All necessary ethical approvals from relevant committees at participating institutions have been obtained. Written informed consent will be obtained from all participants. The findings from this study are expected to inform climate adaptation strategies and emergency response policies to protect pregnant populations from the impacts of extreme heat, both within India and in other similarly affected regions globally. Results are aimed for journal publication, communicate findings to participants in plain language, disseminating information at conferences and events of similar nature.