Acta Biomaterialia最新文献

{"title":"Obesity prolongs the pro-inflammatory response and attenuates bone healing on titanium implants","authors":"Derek Avery ,&nbsp;Lais Morandini ,&nbsp;Luke Sheakley ,&nbsp;Asmaa Alajmi ,&nbsp;Leah Bergey ,&nbsp;Henry J. Donahue ,&nbsp;Rebecca K. Martin ,&nbsp;Rene Olivares-Navarrete","doi":"10.1016/j.actbio.2024.11.040","DOIUrl":"10.1016/j.actbio.2024.11.040","url":null,"abstract":"<div><div>Obesity is a metabolic disease resulting from excess body fat accumulation associated with chronic systemic inflammation. Obesity has been shown to impact the function and activity of neutrophils, macrophages, and T cells, contributing to higher circulating levels of pro-inflammatory cytokines. Biomaterial surface properties such as roughness and hydrophilicity can influence the behavior of immune cells in the peri-implant microenvironment. This study aimed to determine how obesity induced by a high-fat diet (HFD) affects the inflammatory response to modified titanium (Ti) implants and subsequent bone formation. Obese mice had significantly more neutrophils, pro-inflammatory macrophages, and T cells and fewer anti-inflammatory macrophages and mesenchymal stem cells (MSCs) in the peri-implant tissue than lean mice. Obesity also increased circulating adipokines and pro-inflammatory cytokines when compared to lean animals. Bone formation around Ti implants was reduced in obese mice compared to controls. Adoptive transfer of bone marrow cells isolated from obese mice into wild-type mice demonstrated the localized impact of obesity on immune cell function and phenotype, promoting a pro-inflammatory peri-implant microenvironment and attenuating bone formation post-implantation. These results show that obesity significantly affects the inflammatory response to modified Ti implants, prolonging the pro-inflammatory response to the implanted biomaterial and compromising bone formation.</div></div><div><h3>Statement of significance</h3><div>Obesity has been shown to significantly alter physiological processes, including the behavior of immune cells, inducing a state of systemic chronic inflammation. Our study demonstrates that obesity-induced via a high-fat diet alters immune cell response to implanted biomaterials, with increased pro-inflammatory response and attenuated immunomodulation that results in decreased biomaterial integration.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"192 ","pages":"Pages 473-486"},"PeriodicalIF":9.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Employing synchrotron X-ray scattering and microscopy to explore microstructural mysteries in bioresorbable vascular scaffolds","authors":"Jude Cameron ,&nbsp;Tiziana Di Luccio ,&nbsp;Jordan Barr ,&nbsp;Lison Rocher ,&nbsp;Eugene Kim ,&nbsp;Gary H. Menary ,&nbsp;Alex B. Lennon ,&nbsp;Julia A. Kornfield","doi":"10.1016/j.actbio.2024.10.048","DOIUrl":"10.1016/j.actbio.2024.10.048","url":null,"abstract":"<div><div>Crystal structure and morphology dictate the mechanical, thermal, and degradation properties of poly l-lactide (PLLA), the structural polymer of the first clinically approved bioresorbable vascular scaffolds (BVS). New experimental methods are developed to reveal the underlying mechanisms governing structure formation during the crimping step of the BVS manufacturing process. Our research specifically examines the “U-bends” – the region where the curvature is highest and stress is maximised during crimping, which can potentially lead to failure of the device with dramatic consequences on patient life. A custom-made crimping rig operated at a synchrotron beamline enabled collection of wide- and small-angle X-ray scattering (WAXS/SAXS) to probe local variations of the polymer morphology as a function of position in the crest of multiple U-bends with 5 μm resolution <em>in situ</em> after crimping and expansion. Additionally, polarised light microscopy (PLM) images of these deformed U-bends revealed areas with varying stress distribution developed during crimping and expansion. These variations were dependant on the initial biaxial stretching processing step. The integrated X-ray scattering-microscopy approach offered a comprehensive work-flow for uncovering the intricate relationship between processing conditions and the corresponding spatially-resolved semicrystalline morphology of a BVS.</div></div><div><h3>Statement of Significance</h3><div>This research introduces a new method for gaining critical insights into the structural changes that occur during the manufacturing process of bioresorbable vascular scaffolds (BVS). The crimping and expansion of poly l-lactide (PLLA) – the structural material of BVS – are sequential manufacturing steps characterised by highly non-linear deformations at temperature conditions that remain unexplored.</div><div>By utilising synchrotron X-ray scattering techniques alongside polarised light microscopy, we have developed new experimental methods to uncover the mechanisms governing structure formation during processing. This innovative approach not only deepens our understanding of the relationship between processing conditions and polymer morphology but also establishes the foundation for real-time observation methods during crimping and expansion. By improving the design and performance of BVS, this study has the potential to advance cardiovascular treatments and improve patient safety, making it highly relevant and impactful to both scientific research and clinical applications.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"192 ","pages":"Pages 175-188"},"PeriodicalIF":9.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cascade catalytic multilayer modified intraocular lens for enhanced and safer posterior capsule opacification prevention","authors":"Qingqing Jia,&nbsp;Youfei Wei,&nbsp;Yulin Hu,&nbsp;Yuexin Yang,&nbsp;Wenxin Hong,&nbsp;Huiying Huang,&nbsp;Quankui Lin","doi":"10.1016/j.actbio.2024.12.016","DOIUrl":"10.1016/j.actbio.2024.12.016","url":null,"abstract":"<div><div>Posterior capsule opacification (PCO) is the most common complication after cataract surgery. It is primarily caused by the proliferation, migration, and adhesion of residual lens epithelial cells within the capsular bag following phacoemulsification and intraocular lens (IOL) implantation. Although investigations of surface modification onto IOL have partially reduced PCO development in recent years, there are still challenges in long-term efficacy and intraocular biocompatibility. In this study, a cascade catalytic system is constructed using natural enzymes onto mesoporous silica nanoparticles (MSNs), which are subsequently fixed to the surface of IOL through layer-by-layer self-assemble of alternating positive and negative charges. The cascade catalytic reaction is trigged simply by glucose within the pouch to produce reactive oxygen species (ROS) without introducing any toxic drugs or external energy, attempting to minimize the possible toxic side effects to surrounding tissues. In vivo and in vitro experiments indicate the effective inhibition of PCO and favorable intraocular compatibility of the cascade catalytic platform modified IOL. More importantly, the modified IOL retains good optical performance and imaging quality, demonstrating promising prospects for application. This study provides a new possibility for enhanced and safer PCO prevention, playing great significance in clinical treatment.</div></div><div><h3>Statement of Significance</h3><div>Cascade catalytic nanoparticles-loaded multilayer modified IOL is obtained via LbL technique.</div><div>The multilayer coating improves both the loading capacity and the activity of the cascade catalytic nanoparticles.</div><div>The cascade catalytic reaction is trigged by glucose, producing ROS that efficiently induces apoptosis and death of remaining cells on IOL without introducing any toxic drugs or external energy.</div><div>The innovative IOL provides a promising approach for enhanced and safer prevention of PCO.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"192 ","pages":"Pages 248-259"},"PeriodicalIF":9.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Relaxivity study of amino acid-branched radical dendrimers as MRI contrast agents for potential brain tumor imaging","authors":"Yufei Wu ,&nbsp;Vega Lloveras ,&nbsp;Silvia Lope-Piedrafita ,&nbsp;Marta Mulero-Acevedo ,&nbsp;Ana Paula Candiota ,&nbsp;José Vidal-Gancedo","doi":"10.1016/j.actbio.2024.12.006","DOIUrl":"10.1016/j.actbio.2024.12.006","url":null,"abstract":"<div><div>This study introduces a series of water-soluble radical dendrimers (G0 to G5) as promising magnetic resonance imaging (MRI) contrast agents that could potentially address clinical safety concerns associated with current gadolinium-based contrast agents. By using a simplified synthetic approach based on a cyclotriphosphazene core and lysine-derived branching units, we successfully developed a G5 dendrimer containing up to 192 units of 2,2,6,6-Tetramethylpiperidinyloxy (TEMPO) radical. This synthesis offers advantages including ease of preparation, purification, and tunable water solubility through the incorporation of glutamic acid anion residues. Comprehensive characterization using ¹H NMR, FT-IR, and SEC-HPLC confirmed the dendrimers' structures and purity. Electron paramagnetic resonance (EPR) spectroscopy revealed that TEMPO groups in higher generation dendrimers exhibited decreased mobility and stronger spin exchange in their local environments. <em>In vitro</em> MRI showed that relaxivity (<em>r</em>₁) increased with higher dendrimer generations, with G5 exhibiting an exceptionally high <em>r</em>₁ of over 24 mM^-1s^-1. Molecular dynamics simulations provided crucial insights into structure-property relationships, revealing the importance of water accessibility to TEMPO groups for enhancing relaxivity. Vero cell viability assay demonstrated G3 and G3.5 have good biocompatibility. <em>In vivo</em> MRI experiments in mice demonstrated that G3.5 was excreted through the kidneys and selectively accumulated in glioblastoma tumors.</div></div><div><h3>Statement of significance</h3><div>This study explores a class of MRI contrast agents based on organic radical dendrimers as a potential alternative to gadolinium-based agents. We present a simplified synthesis method for water-soluble dendrimers containing up to 192 TEMPO radical units—the highest number achieved to date for this class of compounds—resulting in record-high relaxivity values. Our approach offers easier preparation, purification, and tunable water solubility, representing an improvement over existing methods. Through combined experimental and computational studies, we provide insights into the structure-property relationships governing relaxivity. <em>In vivo</em> experiments demonstrate the dendrimers' potential for glioblastoma imaging, with predominantly renal excretion. This work represents a step towards developing metal-free MRI contrast agents with promising relaxivity and biocompatibility, potentially opening new avenues for diagnostic imaging research.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"192 ","pages":"Pages 461-472"},"PeriodicalIF":9.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142796682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electro-deformation spectroscopy: A unified method for simultaneous electrical and mechanical characterization of single cells","authors":"E Du ,&nbsp;Hongyuan Xu ,&nbsp;Liliana Ponkratova","doi":"10.1016/j.actbio.2024.12.012","DOIUrl":"10.1016/j.actbio.2024.12.012","url":null,"abstract":"<div><div>The intrinsic electrical and mechanical properties of cells are not only valuable biophysical markers reflective of physiological conditions but also play important roles in the development and progression of human diseases. Existing single-cell techniques are restricted to assessing either mechanical or electrical properties. We introduce the development of electro-deformation spectroscopy (EDS), namely the frequency-dependent electro-deformation, as a new method for simultaneous electrical and mechanical characterization of individual cells in suspension. To facilitate the practical use of this technology, we developed a testing procedure that evaluates red blood cells (RBCs) directly from whole blood in a simple microfluidic system, employing an electric field magnitude of 34 kV/m over a frequency range of 15 MHz to 100 kHz. The EDS measurement is performed under stationary conditions without special cell stabilization, at a moderate throughput of 50–100 cells per minute. We develop an experimental-computational framework to decouple cell electromechanics by optimizing the most suitable parameters of the relative permittivity of cell membrane, cytoplasm electrical conductivity, and membrane shear modulus. This technique, tested on RBCs from 4 healthy human samples, revealed membrane relative permittivity of 3.6 – 5.8, membrane shear modulus of 2.2 – 2.8 µN/m, and cytoplasm conductivity of 0.47 – 0.81 S/m. EDS analysis identifies the marked intrasample heterogeneity and individual variability in both cellular electrical and mechanical properties. The EDS framework can be readily used to test RBCs across different species, pathological states, and other cell types of similar structures as RBCs.</div></div><div><h3>Statement of significance</h3><div>This work introduces electro-deformation spectroscopy (EDS) as a unified method for simultaneous electrical and mechanical characterization of single cells in suspension. This is the first-of-its-kind technology for such purposes. EDS can be performed in a simple microfluidic system with minimal sample preparation, making it a convenient and powerful tool for label-free, non-invasive single-cell analysis. We validate the applicability of EDS by measuring the intrasample heterogeneity and individual variability based on the electromechanical parameters of interest for human red blood cells. Single-cell EDS has the potential to enable rapid and reliable detection of cellular changes by diseases or drug treatments and provide insights into the fundamental bioelectromechanical mechanisms of cellular adaptation and dysfunction. This work advances the field of single-cell analysis and contributes to the development of biomaterials and biotechnologies based on cellular electromechanics.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"192 ","pages":"Pages 119-127"},"PeriodicalIF":9.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhalation of macrophage membrane-coated hydrogel microparticles for inflammation alleviation of acute lung injury in vivo","authors":"Liang Song ,&nbsp;Zihe Zhai ,&nbsp;Wei Ouyang ,&nbsp;Jie Ding ,&nbsp;Shuqin Wang ,&nbsp;Shifen Li ,&nbsp;Min Liang ,&nbsp;Feng Xu ,&nbsp;Changyou Gao","doi":"10.1016/j.actbio.2024.12.015","DOIUrl":"10.1016/j.actbio.2024.12.015","url":null,"abstract":"<div><div>Hydrogel microparticles (HMPs) have many advantages for biomedical applications, particularly for minimally invasive therapy, for example, acute lung injury (ALI) that is characterized by high levels of reactive oxygen species (ROS) and pro-inflammatory mediators in the microenvironment. In this study, ROS-scavenging and pro-inflammatory cytokine-neutralizing HMPs were designed and prepared by using a membrane emulsification device. The HMPs were composed of double bond-modified hyaluronic acid and ROS-cleavable hyperbranched poly(acrylate-capped thioketone-containing ethylene glycol) (HBPAK) containing thioketal linkages and unsaturated double bonds. Surface-coating of inflammatory macrophage (M1) cell membranes was performed to obtain the membrane-coated HBPAK HMPs (mem HMPs) via electrostatic force. The mem HMPs exhibited strong ROS-scavenging and anti-inflammatory properties both <em>in vitro</em> and <em>in vivo</em>. After administered by inhalation in an ALI mouse model, the mem HMPs reduced neutrophil infiltration and tissue oxidative damage, thereby alleviating lung inflammation. Our results suggest that the mem HMPs could serve as a potential therapeutic platform for treating inflammatory diseases with high efficiency.</div></div><div><h3>Statement of significance</h3><div>Hydrogel microparticles (HMPs) with minimally invasive delivery are advantageous for acute lung injury (ALI) characterized by high levels of reactive oxygen species (ROS) and pro-inflammatory mediators. Herein, ROS-scavenging and pro-inflammatory cytokine-neutralizing HMPs were prepared by copolymerizing double bond-modified hyaluronic acid and ROS-cleavable hyperbranched poly(acrylate-capped thioketone-containing ethylene glycol) (HBPAK) containing thioketal bonds and unsaturated double bonds in a membrane emulsification device. The HMPs covered with inflammatory macrophage (M1) cell membranes (mem HMPs) exhibited strong ROS-scavenging and anti-inflammation properties, reduced neutrophil infiltration and tissue oxidative damage, thereby alleviating lung inflammation.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"192 ","pages":"Pages 409-418"},"PeriodicalIF":9.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142796677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incorporation of metal-doped silicate microparticles into collagen scaffolds combines chemical and architectural cues for endochondral bone healing","authors":"Janina Stadter ,&nbsp;Andreas Hoess ,&nbsp;Hans Leemhuis ,&nbsp;Aaron Herrera ,&nbsp;Rebecca Günther ,&nbsp;Simone Cho ,&nbsp;Stephanie Diederich ,&nbsp;Gabriela Korus ,&nbsp;Richard Frank Richter ,&nbsp;Ansgar Petersen","doi":"10.1016/j.actbio.2024.12.029","DOIUrl":"10.1016/j.actbio.2024.12.029","url":null,"abstract":"<div><div>Regeneration of large bone defects remains a clinical challenge until today. While existing biomaterials are predominantly addressing bone healing via direct, intramembranous ossification (IO), bone tissue formation via a cartilage phase, so-called endochondral ossification (EO) has been shown to be a promising alternative strategy. However, pure biomaterial approaches for EO induction are sparse and the knowledge how material components can have bioactive contribution to the required cartilage formation is limited. Here, we combined a previously developed purely architecture-driven biomaterial approach with the release of therapeutic metal ions from tailored silicate microparticles. The delivery platform was free of calcium phosphates (CaP) that are known to support IO but not EO and was employed for the release of lithium (Li), magnesium (Mg), strontium (Sr) or zinc (Zn) ions. We identified an ion-specific cellular response in which certain metal ions strongly enhanced cell recruitment into the material and showed superior chondrogenesis and deposition collagen II by human mesenchymal stromal cells (MSCs). At the same time, in some cases microparticle incorporation altered the mechanical properties of the biomaterial with consequences for cell-induced biomaterial contraction and scaffold wall deformation. Collectively, the results suggest that the incorporation of metal-doped silicate microparticles has the potential to further improve the bioactivity of architectured biomaterials for bone defect healing via EO.</div></div><div><h3>Statement of significance</h3><div>Endochondral bone healing, a process that resembles embryonic skeletal development, has gained prominence in regenerative medicine. However, most therapeutic biomaterial strategies are not optimized for endochondral bone healing but instead target direct bone formation through IO. Here, we report on a novel approach to accelerate biomaterial-guided endochondral bone healing by combining cell-guiding collagen scaffolds with therapeutic metal-doped silicate microparticles. While other strategies, such as hypoxia-mimic drugs and iron-chelating biomaterials, have been documented in the literature before to enhance EO, our approach uniquely implements enhanced bioactivity into a previously developed biomaterial strategy for bone defect regeneration. Enhanced cell recruitment into the material and more pronounced chondrogenesis were observed for specific hybrid scaffold formulations, suggesting a high relevance of this new biomaterial for improved endochondral bone healing.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"192 ","pages":"Pages 260-278"},"PeriodicalIF":9.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Super-hydrophilic and super-lubricating Zwitterionic hydrogel coatings coupled with polyurethane to reduce postoperative dura mater adhesions and infections","authors":"Hui Rong ,&nbsp;Shupeng Sun ,&nbsp;Manhua Lu ,&nbsp;Yiqun Zhang ,&nbsp;Lingyuan Liu ,&nbsp;Ziwei Guo ,&nbsp;Zimeng Zhang ,&nbsp;Zhanpeng Ye ,&nbsp;Jianhua Zhang ,&nbsp;Budong Chen ,&nbsp;Shuangyang Li ,&nbsp;Anjie Dong","doi":"10.1016/j.actbio.2024.12.038","DOIUrl":"10.1016/j.actbio.2024.12.038","url":null,"abstract":"<div><div>The dura trauma or large defects due to neurosurgical procedures can result in potential complications. Dural replacements have proven effective to reduce the risk of seizures, meningitis, cerebrospinal fluid leakage, cerebral herniation, and infection. Although various artificial dural patches have been developed, addressing iatrogenic infections and cerebral adhesions resulting from patches implantation remains a challenge. This study employed a network interpenetration modification strategy to introduce super-hydrophilic and super-lubricity zwitterionic hydrogel coatings on polyurethane Neuro-Patch® (NP®) dura mater patch. The successful modification with the hydrogel coating preserved the intrinsic properties of the NP®, such as their anti-leakage and tensile strength capabilities, while effectively reducing biofouling on the surface of the patches. Additionally, by constructing subdural implantation for each dura mater substitute in rabbits, we observed that artificial dura mater patches modified with the hydrogel coating effectively reduced the incidence of postoperative cerebral adhesions and infections. This suggests a promising application prospect of the hydrogel coating in dural repair.</div></div><div><h3>Statement of significance</h3><div>The development of dural substitutes with anti-leakage, anti-adhesion and anti-infection functions is the key to the treatment of dural defects and cerebrospinal fluid leakage during trauma or neurosurgery. In this study, the amphoteric ionic hydrogel coating was firmly modified on the surface of polyurethane with a mild modification process to give the patch super-hydrophilic and super-lubricating properties. The adhesion of non-specific proteins and bacteria is effectively reduced. The rabbit dural defect repair model showed that the introduction of zwitterionic hydrogel coating effectively reduced the occurrence of postoperative infection, and no tissue adhesion was observed. Taken together, this study offers a promising way to enhance the performance of artificial dural patches, potentially benefiting patients undergoing neurosurgery.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"192 ","pages":"Pages 206-217"},"PeriodicalIF":9.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142831190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic insights into mosquito antennal architecture for auditory adaptations","authors":"Adwait A. Trikanad ,&nbsp;Phani Saketh Dasika ,&nbsp;Hoover Pantoja-Sánchez ,&nbsp;Ximena E. Bernal ,&nbsp;Pablo D. Zavattieri","doi":"10.1016/j.actbio.2024.12.031","DOIUrl":"10.1016/j.actbio.2024.12.031","url":null,"abstract":"<div><div>Unlike organisms equipped with tympanal ears, mosquitoes hear using their antennae, which are lightweight sensory structures capable of detecting sound. Here, we study the antennae of two species — <em>Aedes aegypti</em> and <em>Uranotaenia lowii</em> — known to use hearing for different functions. Through the use of geometrically comprehensive computational models, we find that architectural features in the mosquito antenna provide mechanisms that promote the detection of species and sex specific acoustic targets amidst the non-target signals produced by their own wingbeats. Structurally, we find that the increased surface area of sensory hairs provides enhanced sensitivity while the tapering effect of intersegmental variation affects the tuning response. These features result in the highest antennal sensitivity through vibration at specific natural frequency modes that correspond to frequencies associated with their acoustic targets.</div></div><div><h3>Statement of Significance</h3><div>Our study provides valuable insights into the remarkable architectural design of mosquito antennae and its role in auditory adaptations. By dissecting the intricate geometry of antennal architecture in <em>Aedes aegypti</em> and <em>Uranotaenia lowii</em>, we uncover mechanisms that enhance sensitivity to specific acoustic cues while mitigating interference from wingbeat noise. This research builds upon and extends the existing understanding, providing a deeper comprehension of how mosquitoes navigate their acoustic environment. Our findings have significant implications for understanding sensory adaptations in insects and may inspire the development of bioinspired sensing technologies. We believe our work will interest a broad audience by offering new perspectives on the intersection of biomechanics and sensory biology, which can also find applications in the design of bioinspired architected materials.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"192 ","pages":"Pages 165-174"},"PeriodicalIF":9.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TEVAR versus open aortic arch replacement in ex vivo perfused human thoracic aortas","authors":"Masoud Yusefi ,&nbsp;Emmanouil Agrafiotis ,&nbsp;Peter Regitnig ,&nbsp;Günther Laufer ,&nbsp;Gerhard Sommer ,&nbsp;Gerhard A. Holzapfel ,&nbsp;Heinrich Mächler","doi":"10.1016/j.actbio.2024.12.019","DOIUrl":"10.1016/j.actbio.2024.12.019","url":null,"abstract":"&lt;div&gt;&lt;div&gt;This study aims to assess the outcomes of therapeutic options for aortic arch pathologies by comparing thoracic endovascular aortic repair (TEVAR) with open arch replacement (OAR) using woven polyester grafts from a mechanical and biomechanical perspective, with emphasis on &lt;em&gt;ex vivo&lt;/em&gt; perfused human thoracic aortas reproducing heart rate and stroke volume conditions. Eleven non-diseased thoracic aortas from human cadavers were divided into TEVAR (&lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;n&lt;/mi&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;mn&gt;5&lt;/mn&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;) and OAR (&lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;n&lt;/mi&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;mn&gt;6&lt;/mn&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;) and tested using a custom-built mock circulation loop. Pressure, diameter, and stroke volume were monitored during perfusion before and after the intervention. Samples undergoing TEVAR showed a higher ascending systolic pressure post-intervention than OAR (TEVAR: &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mn&gt;137&lt;/mn&gt;&lt;mo&gt;±&lt;/mo&gt;&lt;mn&gt;9&lt;/mn&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; &lt;!--&gt; &lt;!--&gt;mmHg vs OAR: &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mn&gt;126&lt;/mn&gt;&lt;mo&gt;±&lt;/mo&gt;&lt;mn&gt;6&lt;/mn&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; &lt;!--&gt; &lt;!--&gt;mmHg, &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;p&lt;/mi&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;mn&gt;0&lt;/mn&gt;&lt;mo&gt;.&lt;/mo&gt;&lt;mn&gt;017&lt;/mn&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;). After the intervention, a significant discrepancy in the mean pressure differences between the ascending and descending aorta &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;Δ&lt;/mi&gt;&lt;mi&gt;P&lt;/mi&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; was observed (TEVAR: &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mn&gt;9&lt;/mn&gt;&lt;mo&gt;±&lt;/mo&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; &lt;!--&gt; &lt;!--&gt;mmHg vs OAR: &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mn&gt;1&lt;/mn&gt;&lt;mo&gt;±&lt;/mo&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; &lt;!--&gt; &lt;!--&gt;mmHg, &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;p&lt;/mi&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;mn&gt;0&lt;/mn&gt;&lt;mo&gt;.&lt;/mo&gt;&lt;mn&gt;004&lt;/mn&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;). Input impedance at zero frequency, approximating Windkessel resistance, was higher for TEVAR than for OAR (TEVAR: &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mn&gt;1&lt;/mn&gt;&lt;mo&gt;.&lt;/mo&gt;&lt;mn&gt;78&lt;/mn&gt;&lt;mo&gt;±&lt;/mo&gt;&lt;mn&gt;0&lt;/mn&gt;&lt;mo&gt;.&lt;/mo&gt;&lt;mn&gt;04&lt;/mn&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; vs OAR: &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mn&gt;1&lt;/mn&gt;&lt;mo&gt;.&lt;/mo&gt;&lt;mn&gt;66&lt;/mn&gt;&lt;mo&gt;±&lt;/mo&gt;&lt;mn&gt;0&lt;/mn&gt;&lt;mo&gt;.&lt;/mo&gt;&lt;mn&gt;03&lt;/mn&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; &lt;!--&gt; &lt;!--&gt;mmHg&lt;!--&gt; &lt;!--&gt;s/ml, &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;p&lt;/mi&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;mn&gt;0&lt;/mn&gt;&lt;mo&gt;.&lt;/mo&gt;&lt;mn&gt;004&lt;/mn&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;). A correlation was found between the resistance and the negative peak of the time-normalized wave intensity analysis (Kendall’s coefficient &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;τ&lt;/mi&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;mo&gt;−&lt;/mo&gt;&lt;mn&gt;0&lt;/mn&gt;&lt;mo&gt;.&lt;/mo&gt;&lt;mn&gt;35&lt;/mn&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; and &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;p&lt;/mi&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;mn&gt;0&lt;/mn&gt;&lt;mo&gt;.&lt;/mo&gt;&lt;mn&gt;023&lt;/mn&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;). Another correlation was observed between resistance and &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;Δ&lt;/mi&gt;&lt;mi&gt;P&lt;/mi&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; (&lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;τ&lt;/mi&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;mn&gt;0&lt;/mn&gt;&lt;mo&gt;.&lt;/mo&gt;&lt;mn&gt;51&lt;/mn&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;, &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;p&lt;/mi&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;mn&gt;0&lt;/mn&gt;&lt;mo&gt;.&lt;/mo&gt;&lt;mn&gt;001&lt;/mn&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;). Looking at the replication of heart rate and stroke volume over the course of the study, the observed differences can largely be attributed to the type of intervention. The results suggest that TEVAR has adverse effects compared to OAR, particu","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"192 ","pages":"Pages 140-150"},"PeriodicalIF":9.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}