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Combined toxicity of nanoplastics and sodium fluoride to zebrafish liver: Impact on gut-liver axis homeostasis and lipid metabolism 纳米塑料和氟化钠对斑马鱼肝脏的联合毒性:对肠-肝轴稳态和脂质代谢的影响
IF 4.3 2区 环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2025-12-11 DOI: 10.1016/j.aquatox.2025.107682
Zizheng Wang , Boran Zhou , Yingxue Zhang, Yufei Cao, Yiqiang Zhang, Xu Han, Yu Wang, Hongjing Zhao
Nanoplastics and fluorides are widespread environmental pollutants, but their combined exposure risks to aquatic organisms and humans remain unclear. Studying their combined effect of inducing toxicity on the gut-liver axis at environmentally relevant concentrations is critical. Using zebrafish, this study evaluated toxic effects and mechanisms of single/combined exposure to 0.1 mg/L nanoplastics (NPs) and 15 mg/L sodium fluoride (NaF). Integrating network toxicology and in vivo validation, combined exposure significantly disrupted intestinal structure, increased permeability, and disturbed microbiota balance. Gut microbiota dysbiosis mediated hepatic lipid metabolism disorders via the gut-liver axis by activating the TLR4/NF-κB pathway, inducing liver inflammation, oxidative stress, and hepatocyte apoptosis. The observed toxic effects are consistent with gut-liver axis homeostasis disruption, though definitive causal links have not been established. This reveals their combined effect of inducing liver injury by interfering with gut-liver axis homeostasis, providing a theoretical basis for assessing ecological risks of compound pollutants and scientific references for pollution management and aquatic ecological protection.
纳米塑料和氟化物是广泛存在的环境污染物,但它们对水生生物和人类的综合暴露风险尚不清楚。研究它们在环境相关浓度下对肠-肝轴诱导毒性的综合效应是至关重要的。本研究以斑马鱼为研究对象,评估了单一/联合暴露于0.1 mg/L纳米塑料(NPs)和15 mg/L氟化钠(NaF)的毒性作用和机制。综合网络毒理学和体内验证,联合暴露显着破坏了肠道结构,增加了渗透性,扰乱了微生物群平衡。肠道菌群失调通过激活TLR4/NF-κB通路,通过肠-肝轴介导肝脏脂质代谢紊乱,诱导肝脏炎症、氧化应激和肝细胞凋亡。观察到的毒性作用与肠-肝轴稳态破坏一致,尽管明确的因果关系尚未建立。揭示了它们通过干扰肠肝轴稳态诱导肝损伤的综合作用,为评价复合污染物的生态风险提供了理论依据,为污染治理和水生生态保护提供了科学参考。
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引用次数: 0
Microplastics and bisphenol A exposure induce hepatopancreatic damage and lipid metabolism disorders in Portunus trituberculatus 微塑料和双酚A暴露可诱导三瘤鲳肝胰腺损伤和脂质代谢紊乱
IF 4.3 2区 环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2025-12-09 DOI: 10.1016/j.aquatox.2025.107681
Tao Zhang , Biqing Wen , Xunjie Huo , Jiayuan Ren , Xuerui Ge , Xiaocong Chen
This study exposed female Portunus trituberculatus crabs (developmental stages III-IV) to microplastics (MPs) and bisphenol A (BPA) individually or combined for 21 days. Assessments included growth, histology, enzyme/gene expression, and metabolomics. Both MPs and BPA caused hepatopancreatic damage and lipid accumulation but via distinct mechanisms. MPs groups showed downregulated acetyl-CoA carboxylase (ACC) gene expression and upregulated fatty acid transport protein (FATP) genes, with reduced N-acetyl-d-glucosamine synthesis, suggesting disrupted energy metabolism (e.g., nucleotide sugar synthesis and ABC transport). BPA groups showed similarly downregulated ACC but upregulated FATP and Fatty Acid-Binding Protein (FABP) genes. Metabolomic shifts included decreased uric acid/prostaglandin F2α and increased glycochenodeoxycholic acid/inositol-1,3-bisphosphate, indicating estrogenic effects and hormonal imbalance. Combined exposure exacerbates hepatopancreatic injury and lipid metabolism disorders through complex mechanisms of action, highlighting heightened risks to aquatic ecosystems and potential human health impacts. The study underscores MPs and BPA as dual threats with unique and compounded toxicity pathways.
本研究将发育阶段为III-IV期的雌性三瘤蟹单独或联合暴露于微塑料(MPs)和双酚A (BPA)中21天。评估包括生长、组织学、酶/基因表达和代谢组学。MPs和BPA均引起肝胰腺损伤和脂质积累,但机制不同。MPs组乙酰辅酶a羧化酶(ACC)基因表达下调,脂肪酸转运蛋白(FATP)基因表达上调,n -乙酰-d-葡萄糖胺合成减少,提示能量代谢(如核苷酸糖合成和ABC转运)受到干扰。BPA组ACC基因同样下调,但FATP和脂肪酸结合蛋白(FABP)基因上调。代谢组学变化包括尿酸/前列腺素F2α降低,糖鹅去氧胆酸/肌醇-1,3-二磷酸升高,表明雌激素作用和激素失衡。通过复杂的作用机制,复合暴露加剧了肝胰损伤和脂质代谢紊乱,突出了对水生生态系统和潜在人类健康影响的更高风险。该研究强调MPs和BPA是具有独特和复合毒性途径的双重威胁。
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引用次数: 0
Are alternatives to polyethylene less toxic than conventional polyethylene? A case study of MP toxicity on Scrobicularia plana using an Integrative Biomarker Response index (IBR-T) 聚乙烯的替代品是否比传统聚乙烯毒性更小?应用综合生物标志物反应指数(IBR-T)研究MP对平面隐索菌的毒性
IF 4.3 2区 环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2025-12-09 DOI: 10.1016/j.aquatox.2025.107680
Clémentine Labbé , Isabelle Métais , Hanane Perrein-Ettajani , Mohammed Mouloud , Antoine Le Guernic , Oihana Latchere , Nicolas Manier , Amélie Châtel
Microplastics (MP) are now widely contaminating multiple ecosystems. To mitigate this pollution, the development of plastic alternatives is being promoted. However, scientific data on the absence of toxicity of these alternatives is still lacking. The present study assesses the impact of petroleum-based fragmentable MP, oxo-polyethylene (oxoPE), and biobased biodegradable MP, polylactic acid (PLA), on the bivalve Scrobicularia plana. Clams were exposed for 21 days to environmental concentrations (0.008, 10 and 100 μg L−1) of both PLA and oxoPE MP and impacts were evaluated from individual to molecular levels. The effects of alternative MP were then compared with polyethylene MP ones, resulted from a previous study. An Integrative Biomarker Response - Threshold index (IBR-T) was calculated to assess the differences in toxicity between alternative and conventional plastics. An impact of PLA and oxoPE MP was shown on energy reserves, with a reduction in glycogen concentration in organisms exposed to the highest concentration (100 μg L−1). A decrease in burrowing was also reported in S. plana exposed to 0.008 and 100 µg L−1 of PLA MP. At cellular and molecular levels, catalase (CAT) and glutathione-S-transferases (GST) activities decreased after exposure to PLA MP (10 and 100 μg L−1 respectively) suggesting a disruption of antioxidant and detoxication systems, while no impact was noted on DNA damage, whatever the biodegradable MP. Analysis using IBR-T revealed greater effects of PLA (100 μg L−1) on S. plana physiology compared to the different types of PE MP tested, while oxoPE MP showed limited toxicity. These findings support the importance of assessing the toxic effects of biodegradable and fragmentable MP to evaluate whether they can really be considered as a reliable alternative to conventional plastics.
微塑料(MP)正在广泛污染多种生态系统。为了减轻这种污染,人们正在推动塑料替代品的开发。然而,关于这些替代品没有毒性的科学数据仍然缺乏。本研究评估了石油基可破碎MP,氧聚乙烯(oxoPE)和生物基可生物降解MP,聚乳酸(PLA)对双壳类浮游生物的影响。将蛤蜊暴露于环境浓度(0.008、10和100 μg L−1)的PLA和oxoPE MP中21天,从个体到分子水平评估其影响。然后将替代MP的效果与聚乙烯MP的效果进行比较,这是先前研究的结果。计算了综合生物标志物反应阈值指数(IBR-T)来评估替代塑料和传统塑料之间的毒性差异。PLA和oxoPE MP对能量储备有影响,暴露于最高浓度(100 μg L−1)的生物体中糖原浓度降低。暴露于0.008和100µg L−1 PLA MP的拟南鲷的穴居也有所减少。在细胞和分子水平上,暴露于PLA MP(分别为10和100 μg L−1)后,过氧化氢酶(CAT)和谷胱甘肽- s转移酶(GST)活性下降,表明抗氧化和解毒系统受到破坏,而对DNA损伤没有影响,无论可生物降解的MP是什么。IBR-T分析显示,与不同类型的PE MP相比,PLA (100 μg L−1)对平面葡萄树生理的影响更大,而oxoPE MP的毒性有限。这些发现支持了评估可生物降解和可破碎MP的毒性作用的重要性,以评估它们是否真的可以被认为是传统塑料的可靠替代品。
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引用次数: 0
Interactive effects of nonylphenol ethoxylate (NPE) exposure and thermal stress on oxidative stress biomarkers and neurotoxicity in bullfrog tadpoles 壬基酚聚氧乙烯酸(NPE)暴露和热应激对牛蛙蝌蚪氧化应激生物标志物和神经毒性的相互作用
IF 4.3 2区 环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2025-12-08 DOI: 10.1016/j.aquatox.2025.107679
Yan Costa Gonçalves , Suzana Luisa Alves Fernandes , Francisco Tadeu Rantin , Ana Lúcia Kalinin , Diana Amaral Monteiro
The global decline in amphibian populations has been well documented and is driven by multiple environmental stressors. Among these, the synergistic effects of chemical pollution and rising water temperatures can significantly contribute to ecological disturbances. Nonylphenol ethoxylate (NPE), a surfactant widely used in sanitary and agrochemical products, has been detected in aquatic ecosystems. Despite its ecological relevance, the sublethal effects of NPE on amphibians, particularly under conditions of thermal stress, remain poorly understood. This study evaluated the effects of acute exposure (48 h) to an environmentally relevant concentration of NPE (30 µg L⁻¹) on Aquarana catesbeiana tadpoles under two thermal regimes (25 °C and 30 °C), including the potential interaction between these conditions. We assessed antioxidant enzymatic activities (superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase), glutathione levels, and oxidative stress biomarkers (lipid peroxidation and protein carbonylation) in hepatic, branchial, and muscular tissues. Neurotoxicity was assessed via acetylcholinesterase activity measurements in brain and muscle tissues. NPE exposure caused hepatotoxicity, oxidative damage and redox imbalance in gills and muscle, as well as inhibition of muscular acetylcholinesterase activity. Elevated temperature further depleted branchial antioxidant defenses, intensified oxidative stress in hepatic and muscular tissues, and induced central neurotoxicity. These findings demonstrate that sublethal exposure to NPE compromises redox balance and cholinergic function in a tissue-specific and temperature-dependent manner. The absence of regulatory limits for NPE in several countries represents a critical ecological threat, highlighting the urgent need to update aquatic environmental guidelines in the context of global warming.
全球两栖动物数量的减少已经有了很好的记录,这是由多种环境压力因素造成的。其中,化学污染和水温升高的协同效应对生态干扰有显著的促进作用。壬基酚聚氧乙酸酯(NPE)是一种广泛应用于卫生和农化产品的表面活性剂,在水生生态系统中被检测到。尽管与生态相关,但NPE对两栖动物的亚致死效应,特别是在热应激条件下,仍然知之甚少。本研究评估了急性暴露(48小时)于环境相关浓度的NPE (30 μ g L - 1)对两种热环境(25°C和30°C)下的Aquarana catesbeiana蝌蚪的影响,包括这些条件之间潜在的相互作用。我们评估了肝脏、鳃和肌肉组织中的抗氧化酶活性(超氧化物歧化酶、过氧化氢酶、谷胱甘肽s -转移酶和谷胱甘肽过氧化物酶)、谷胱甘肽水平和氧化应激生物标志物(脂质过氧化和蛋白质羰基化)。通过测量大脑和肌肉组织中的乙酰胆碱酯酶活性来评估神经毒性。NPE暴露引起肝毒性、鳃和肌肉氧化损伤和氧化还原失衡,以及肌肉乙酰胆碱酯酶活性抑制。升高的温度进一步削弱了鳃抗氧化防御,加剧了肝脏和肌肉组织的氧化应激,并诱发了中枢神经毒性。这些发现表明,亚致死暴露于NPE以组织特异性和温度依赖的方式损害氧化还原平衡和胆碱能功能。一些国家缺乏对NPE的监管限制,这是一种严重的生态威胁,突出表明迫切需要在全球变暖的背景下更新水生环境准则。
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引用次数: 0
Micro/nanoplastic-induced stress in microalgae: Latest laboratory evidence and knowledge gaps 微藻中微/纳米塑性诱导的应力:最新的实验室证据和知识空白
IF 4.3 2区 环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2025-12-06 DOI: 10.1016/j.aquatox.2025.107677
Amina Antonacci , Vincenzo Vedi , Sara Colella , Alessia Di Fraia , Manuela Rossi , Gabriella Fiorentino , Viviana Scognamiglio
Pollution by micro- and nanoplastics (MNPs) is a major concern today, with direct consequences for human health and the environment. Microalgae, among the main primary producers in aquatic ecosystems, suffer from MNPs contamination due to the worrying amount of plastic waste and its persistence in the environment. This problem has mobilized international organizations and raised awareness among the scientific community for the identification of effective solutions. Policies aimed at reducing plastic products, waste management, and recycling are attempting to limit this problem; however, plastic pollution appears to be irreversible. Therefore, a critical analysis of the effects of MNPs on various microorganisms (e.g., microalgae) is urgently needed. Therefore, the objective of this review was to identify the impact of micro- and nanoplastics on microalgal populations, based on the currently available literature. Particular attention was paid to available laboratory studies on MNPs effects on microalgae physiology - as growth rate, pigment content, photosynthetic activity, oxidative stress - and morphology, with the aim of providing an update on the state of the art.
微塑料和纳米塑料造成的污染是当今的一个主要问题,对人类健康和环境产生直接影响。微藻是水生生态系统中主要的初级生产者之一,由于令人担忧的塑料废物数量及其在环境中的持久性,微藻受到MNPs污染。这一问题调动了国际组织,提高了科学界的认识,以便找出有效的解决办法。旨在减少塑料制品、废物管理和回收的政策正试图限制这一问题;然而,塑料污染似乎是不可逆转的。因此,迫切需要对MNPs对各种微生物(如微藻)的影响进行批判性分析。因此,本综述的目的是在现有文献的基础上确定微塑料和纳米塑料对微藻种群的影响。特别注意了关于MNPs对微藻生理(如生长速度、色素含量、光合活性、氧化应激)和形态的影响的现有实验室研究,目的是提供最新的技术状况。
{"title":"Micro/nanoplastic-induced stress in microalgae: Latest laboratory evidence and knowledge gaps","authors":"Amina Antonacci ,&nbsp;Vincenzo Vedi ,&nbsp;Sara Colella ,&nbsp;Alessia Di Fraia ,&nbsp;Manuela Rossi ,&nbsp;Gabriella Fiorentino ,&nbsp;Viviana Scognamiglio","doi":"10.1016/j.aquatox.2025.107677","DOIUrl":"10.1016/j.aquatox.2025.107677","url":null,"abstract":"<div><div>Pollution by micro- and nanoplastics (MNPs) is a major concern today, with direct consequences for human health and the environment. Microalgae, among the main primary producers in aquatic ecosystems, suffer from MNPs contamination due to the worrying amount of plastic waste and its persistence in the environment. This problem has mobilized international organizations and raised awareness among the scientific community for the identification of effective solutions. Policies aimed at reducing plastic products, waste management, and recycling are attempting to limit this problem; however, plastic pollution appears to be irreversible. Therefore, a critical analysis of the effects of MNPs on various microorganisms (e.g., microalgae) is urgently needed. Therefore, the objective of this review was to identify the impact of micro- and nanoplastics on microalgal populations, based on the currently available literature. Particular attention was paid to available laboratory studies on MNPs effects on microalgae physiology - as growth rate, pigment content, photosynthetic activity, oxidative stress - and morphology, with the aim of providing an update on the state of the art.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"291 ","pages":"Article 107677"},"PeriodicalIF":4.3,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145689952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Size-dependent and tissue specific accumulation of polystyrene microplastics and nanoplastics in zebrafish 聚苯乙烯微塑料和纳米塑料在斑马鱼体内的大小依赖性和组织特异性积累
IF 4.3 2区 环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2025-12-05 DOI: 10.1016/j.aquatox.2025.107678
Min Su , Dangen Gu , Le Liang , Zhendong Zhou , Chenchen Zhu , Jiaoyang Qi , Pu Wu , Tian Xu , Zhongguan Jiang
As emerging pollutants, micro-nanoplastics (MNPs), have been widely detected in aquatic ecosystems. When engaging with fish species, MNPs can be initially accumulated in fish gills and intestinal tract, followed by subsequent transportation to other tissues and organs through the circulatory system. However, the tissue-specific accumulation of MNPs in relation to size differences remains uncertain. In this study, continuous exposure experiments with MNPs of different particle sizes were conducted to evaluate tissue-specific accumulation and to examine associated biochemical responses. Generally, fish intestine and gills accumulated MNPs of all size, whereas fish muscle and brain only accumulated nano-sized MNPs. Specifically, fish muscle and brain showed significantly higher MNPs enrichment of 25 nm (vs. 250 nm), since they can easily pass through the intercellular spaces or be endocytosed within cells. In terms of time-dependent accumulations, fish intestines and gills exhibited rapid initial uptake followed by fluctuating variations of MNPs as exposing lasted, since these organs possess both a high capacity for MNP accessibility and elimination. However, fish liver and brain showed a monotonic increase in MNPs accumulation with continuous exposure, which can be ascribed to the difficulty in draining the metabolic waste of these organs. When examining toxic effects, both AChE and CYP450 enzyme activities in all exposure groups were significantly higher than the control group, indicating that MNPs triggered neurotoxicity and metabolic detoxification. Our study highlights considering size and tissue-specific accumulations of MNPs when planning MNPs control for fish health and fishery products safety.
微纳米塑料(MNPs)作为一种新兴的污染物,在水生生态系统中被广泛发现。与鱼类接触时,MNPs最初可在鱼鳃和肠道中积累,随后通过循环系统运输到其他组织和器官。然而,MNPs的组织特异性积累与大小差异的关系仍然不确定。在这项研究中,不同粒径的MNPs进行了连续暴露实验,以评估组织特异性积累并检查相关的生化反应。一般来说,鱼的肠道和鳃会积累各种大小的MNPs,而鱼的肌肉和大脑只会积累纳米大小的MNPs。具体来说,鱼类肌肉和大脑的MNPs富集程度明显高于25 nm (250 nm),因为它们可以很容易地通过细胞间隙或在细胞内被内吞。就时间依赖性积累而言,鱼的肠道和鳃表现出快速的初始吸收,随后随着暴露时间的持续,MNP的波动变化,因为这些器官具有高的MNP可及性和消除能力。然而,随着持续暴露,鱼肝脏和大脑的MNPs积累单调增加,这可能归因于这些器官的代谢废物难以排出。在检测毒性作用时,所有暴露组的AChE和CYP450酶活性均显著高于对照组,表明MNPs触发了神经毒性和代谢解毒。我们的研究强调,在规划MNPs控制以保证鱼类健康和渔业产品安全时,应考虑MNPs的大小和组织特异性积累。
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引用次数: 0
A whale in a well: Co-exposure of a persistent organic pollutant mixture and cetacean morbillivirus on killer whale (Orcinus orca) primary fibroblasts 井中的鲸鱼:持久性有机污染物混合物和鲸类麻疹病毒对虎鲸(Orcinus orca)原代成纤维细胞的共同暴露
IF 4.3 2区 环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2025-12-04 DOI: 10.1016/j.aquatox.2025.107671
Helena Costa, Maud Van Essche, Juliane A. Riedel, Akash Gupta, Audun H. Rikardsen, Anders Goksøyr, Pierre Blévin, Mikael Harju, Laura Pirard, Susan Bengston Nash, Sofie Sødestrøm, Courtney A. Waugh
Killer whales (Orcinus orca) accumulate high levels of persistent organic pollutants (POPs), which have been linked to immunomodulation. Over the past decades, large-scale mortality events associated with cetacean morbillivirus (CeMV) have affected cetacean populations, and concerns have been raised about the role of contaminants in exacerbating these outbreaks. However, establishing cause-effect relationships in free-roaming cetaceans remains a significant challenge. In vitro approaches present unique potential for furthering our understanding of the effects of multiple environmental stressors in marine mammal health. In this study, we used primary fibroblasts cultured from wild Norwegian killer whale skin biopsies (n = 6) to assess how exposure to POP mixtures affects cell viability and CeMV replication. Our findings demonstrate that CeMV successfully replicates in killer whale fibroblasts, with the viral replication significantly increasing over the duration of the experiment. POP exposure led to a concentration-dependent decrease in cell viability and a significant increase in viral replication. These results validate killer whale primary fibroblasts as a valuable in vitro tool for the study of co-exposure of POPs and morbillivirus on toothed cetaceans. Moreover, these findings support the need for further research to confirm the role of contaminants in intensifying the severity of CeMV infections in the wild.
虎鲸(Orcinus orca)积累了高水平的持久性有机污染物(POPs),这与免疫调节有关。在过去的几十年里,与鲸类麻疹病毒(CeMV)相关的大规模死亡事件影响了鲸类种群,人们对污染物在加剧这些暴发中的作用表示关注。然而,在自由漫游的鲸类动物中建立因果关系仍然是一个重大挑战。体外方法为进一步了解多种环境应激源对海洋哺乳动物健康的影响提供了独特的潜力。在这项研究中,我们使用野生挪威虎鲸皮肤活检培养的原代成纤维细胞(n = 6)来评估暴露于POP混合物如何影响细胞活力和CeMV复制。我们的研究结果表明,CeMV在虎鲸成纤维细胞中成功复制,并且随着实验的持续时间,病毒复制显著增加。POP暴露导致细胞活力呈浓度依赖性下降,病毒复制显著增加。这些结果验证了虎鲸原代成纤维细胞作为一种有价值的体外工具,用于研究持久性有机污染物和麻疹病毒对齿鲸类动物的共同暴露。此外,这些发现支持了进一步研究的必要性,以确认污染物在加剧野生CeMV感染严重程度中的作用。
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引用次数: 0
Combined effects of benzotriazole ultraviolet stabilizers and cadmium on physiological performance of marine dinoflagellate Akashiwo sanguinea 苯并三唑紫外线稳定剂和镉对赤潮海洋鞭毛藻生理性能的联合影响
IF 4.3 2区 环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2025-12-03 DOI: 10.1016/j.aquatox.2025.107672
Tiantian Chen , Jiaqi Chen , Wenlong Dong , Shuqun Song , Weijun Tian , Caiwen Li
As emerging contaminants, benzotriazole ultraviolet stabilizers (BUVSs) have been frequently detected in aquatic environments, which usually co-occur with heavy metals and cause complex toxicity to aquatic organisms. However, the specific role of BUVSs in the combined toxicity remains poorly understood. Herein, a harmful marine dinoflagellate Akashiwo sanguinea was used to explore the individual and combined toxicities of UV-234 and cadmium (Cd2+). Exposure to UV-234 at an environmental concentration (10 μg L−1) slightly inhibited algal growth (P > 0.05). Individual exposure to both low (1.77 mg L−1) and high (5.30 mg L−1) concentrations of Cd2+ significantly impaired algal photosynthesis by altering photosynthetic pigments, disrupting energy absorption, dissipation and trapping, reaction center activation, and electron transport, thereby inducing oxidative stress, and up-regulated pyruvate metabolism and the tricarboxylic acid cycle. Notably, co-exposure with UV-234 mitigated the toxic effects induced by Cd2+, and caused weaker inhibition of algal growth via inducing less substantial oxidative damage. These findings highlight the significant influence of UV-234 and Cd2+ co-exposure on marine dinoflagellates, providing new insights into the joint toxicity mechanisms and a scientific basis for environmental risk assessment of emerging BUVSs.
苯并三唑类紫外线稳定剂(BUVSs)作为一种新兴污染物,在水生环境中被检测到较多,通常与重金属共存,对水生生物具有复杂的毒性。然而,BUVSs在联合毒性中的具体作用仍然知之甚少。本文以一种有害的海洋鞭毛藻赤潮(Akashiwo sanguinea)为研究对象,探讨了UV-234和镉(Cd2+)的个体毒性和联合毒性。暴露于环境浓度(10 μg L−1)的UV-234对藻类生长有轻微抑制作用(P > 0.05)。个体暴露于低(1.77 mg L−1)和高(5.30 mg L−1)浓度的Cd2+中,通过改变光合色素、破坏能量吸收、消散和捕获、反应中心激活和电子传递,从而诱导氧化应激,并上调丙酮酸代谢和三羧酸循环,显著损害了藻类的光合作用。值得注意的是,与UV-234共暴露可减轻Cd2+诱导的毒性作用,并通过诱导较少的实质性氧化损伤而减弱对藻类生长的抑制作用。这些发现突出了UV-234和Cd2+共同暴露对海洋鞭毛藻的显著影响,为新出现的BUVSs的联合毒性机制提供了新的见解,并为环境风险评估提供了科学依据。
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引用次数: 0
Acute disturbance, but chronic re-equilibration of the oyster metabolome to 17β-estradiol and nonylphenol exposure 牡蛎代谢组对17β-雌二醇和壬基酚暴露的急性干扰,但慢性再平衡
IF 4.3 2区 环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2025-12-01 DOI: 10.1016/j.aquatox.2025.107663
Sazal Kumar , Wayne A. O’Connor , Allison C. Luengen , Frederic D.L. Leusch , Steve D. Melvin , Chenglong Ji , Junfei Zhan , Geoff R. MacFarlane
In estuaries, aquatic organisms are often exposed to estrogenic endocrine disrupting chemicals (EEDCs), including 17β-estradiol (E2) and nonylphenol (NP), which affect physiology and metabolism. This study evaluated metabolic profiles of Sydney rock oysters (Saccostrea glomerata) using 1HNMR -based metabolomics after acute (14 days), pulse (14 days exposure followed by 14 days depuration), and chronic (28 days) exposure of E2 at 200 ng/L and NP at 5000 ng/L. Only acute exposure to both E2 and NP led to marked metabolic perturbations. Energy and stress-related metabolites including adenosine monophosphate, succinate, acetoacetate, and glutamate significantly increased in acute treatments compared to controls, suggesting heightened energy demand to cope with oxidative and osmotic stress. However, the metabolites from pulse and chronic exposure treatments were not significantly different from the control. Such responses highlight a time-dependent adaptation of molluscs, similar to depuration. E2 is expected to be more rapidly metabolised in molluscs than NP, leading to comparatively slower metabolic adaptation of molluscs to NP exposure. Finally, this study emphasizes that oysters have a time-dependent adaptive mechanism to cope with EEDC exposure.
在河口,水生生物经常暴露于雌激素内分泌干扰物质(EEDCs)中,包括17β-雌二醇(E2)和壬基酚(NP),这些物质会影响生理和代谢。本研究使用基于1HNMR的代谢组学方法评估了悉尼岩牡蛎(Saccostrea glomerata)在急性(14天)、脉冲(14天暴露后14天去除)和慢性(28天)暴露于200 ng/L E2和5000 ng/L NP后的代谢谱。只有急性暴露于E2和NP才会导致明显的代谢紊乱。与对照组相比,急性治疗组能量和应激相关代谢物(包括单磷酸腺苷、琥珀酸、乙酰乙酸和谷氨酸)显著增加,表明应对氧化和渗透应激的能量需求增加。然而,脉冲和慢性暴露处理的代谢物与对照组没有显著差异。这种反应突出了软体动物的时间依赖性适应,类似于净化。E2在软体动物体内的代谢速度预计比NP更快,导致软体动物对NP暴露的代谢适应相对较慢。最后,本研究强调牡蛎具有时间依赖性的适应机制来应对EEDC暴露。
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引用次数: 0
The effects of fluoxetine and metformin pollution on phenotypic traits and gene expression in Daphnia magna 氟西汀和二甲双胍污染对大水蚤表型性状和基因表达的影响
IF 4.3 2区 环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2025-12-01 DOI: 10.1016/j.aquatox.2025.107664
Anaïs H.R. Belfor, S. Schaack
Interest in the effects of pharmaceutical pollution on aquatic habitats has expanded with the growing number and increased distribution of drugs worldwide. In this study, we perform an experiment to examine the effects of two drugs, fluoxetine (known commercially as the anti-depressant Prozac™) and metformin (a widely-used diabetes medication), both of which are common freshwater contaminants. We investigated the effects of the two drugs alone and in combination on Daphnia magna in both in crowded and non-crowded conditions in order to understand how pharmaceutical pollution and naturally-occurring environmental cues might interact to shape phenotypic traits and gene expression. We assayed fecundity, respiration, transgenerational effects, and gene expression levels for three genes. Pharmaceuticals affected offspring, respiration, and gene expression, while crowding affected fecundity. Specifically, fluoxetine induced male production and metformin made offspring sickly. Overall, these drugs and their combination had detectable impacts on many traits, and in some cases the effects depended on crowding conditions. Daphnia, a model system in ecology and ecotoxicology, provides myriad insights into the effects of pollutants, both because of its key role in freshwater food webs and its ability to serve as an experimental system to determine sublethal and lethal effects. Our findings contribute to our current understanding of pharmaceutical pollution and suggest that investigating the risks using more real-world scenarios is important for the maintenance of freshwater drinking supplies and freshwater ecosystems.
随着世界范围内药物数量的增加和分布的增加,人们对药物污染对水生生境的影响的兴趣也越来越大。在这项研究中,我们进行了一项实验来检验两种药物的影响,氟西汀(商业上称为抗抑郁药百忧解™)和二甲双胍(一种广泛使用的糖尿病药物),这两种药物都是常见的淡水污染物。我们研究了两种药物单独和联合在拥挤和非拥挤条件下对大水蚤的影响,以了解药物污染和自然发生的环境线索如何相互作用来塑造表型性状和基因表达。我们分析了三个基因的繁殖力、呼吸作用、跨代效应和基因表达水平。药物影响后代、呼吸和基因表达,而拥挤影响繁殖力。具体来说,氟西汀诱导雄性繁殖,二甲双胍使后代患病。总的来说,这些药物及其组合对许多性状有可检测的影响,在某些情况下,影响取决于拥挤条件。水蚤是生态学和生态毒理学的一个模型系统,它为污染物的影响提供了无数的见解,这既是因为它在淡水食物网中的关键作用,也是因为它有能力作为一个实验系统来确定亚致死和致死效应。我们的研究结果有助于我们目前对药物污染的理解,并建议使用更多的真实场景来调查风险,这对于维持淡水饮用供应和淡水生态系统非常重要。
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引用次数: 0
期刊
Aquatic Toxicology
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