Nguyen Giang Tuyet, Nguyen Hien Duc, Huynh Loan Thi, Phan Phuc Thi, Nguyen Dai Van, T. Shimogiri, Do Khoa Vo
Serum profile is generally considered as comprehensive data which directly reflected animal health and their potential resistance to environmental, nutritional and pathological stress. The present study aimed to provide physiological reference values for selected biochemical parameters in Noi chickens, a famous Vietnamese native breed, at 56 days old. The collected blood samples of 355 Noi birds (164 males and 191 females) were used to evaluate biochemical serum profile. Glucose, total protein, albumin, globulin, albumin/globulin ratio, triglycerides, cholesterol, creatinine, and uric acid in the serum were measured. The variation in these values arising from different sexes was also investigated. The results indicated that there were no differences (P>0.05) in the assessed indices between male and female Noi chickens. In overall for the whole group, these values were obtained as 250.86 mg/dL, 7.34 g/dL, 3.40 g/dL, 3.94 g/dL, 1.75, 148.54 mg/dL, 190.86 mg/dL, 0.44 mg/dL, and 1.44 mg/dL, respectively. The relationship among the biochemical parameters showed relatively low coefficients (r=0.79 in maximum). The females obtained higher correlation coefficients between levels of total protein and glucose (r=0.42) as well as between total protein and globulin (r=0.79), compared to the males. It was concluded that the male and female chickens performed similar values of serum indices. The contribution of this study might expand the knowledge on the biochemical profile and improved breeding strategies of Vietnamese indigenous Noi chickens.
{"title":"Serum biochemical properties of Vietnamese indigenous Noi chicken at 56 days old","authors":"Nguyen Giang Tuyet, Nguyen Hien Duc, Huynh Loan Thi, Phan Phuc Thi, Nguyen Dai Van, T. Shimogiri, Do Khoa Vo","doi":"10.2298/bah2003329g","DOIUrl":"https://doi.org/10.2298/bah2003329g","url":null,"abstract":"Serum profile is generally considered as comprehensive data which directly\u0000 reflected animal health and their potential resistance to environmental,\u0000 nutritional and pathological stress. The present study aimed to provide\u0000 physiological reference values for selected biochemical parameters in Noi\u0000 chickens, a famous Vietnamese native breed, at 56 days old. The collected\u0000 blood samples of 355 Noi birds (164 males and 191 females) were used to\u0000 evaluate biochemical serum profile. Glucose, total protein, albumin,\u0000 globulin, albumin/globulin ratio, triglycerides, cholesterol, creatinine,\u0000 and uric acid in the serum were measured. The variation in these values\u0000 arising from different sexes was also investigated. The results indicated\u0000 that there were no differences (P>0.05) in the assessed indices between male\u0000 and female Noi chickens. In overall for the whole group, these values were\u0000 obtained as 250.86 mg/dL, 7.34 g/dL, 3.40 g/dL, 3.94 g/dL, 1.75, 148.54\u0000 mg/dL, 190.86 mg/dL, 0.44 mg/dL, and 1.44 mg/dL, respectively. The\u0000 relationship among the biochemical parameters showed relatively low\u0000 coefficients (r=0.79 in maximum). The females obtained higher correlation\u0000 coefficients between levels of total protein and glucose (r=0.42) as well as\u0000 between total protein and globulin (r=0.79), compared to the males. It was\u0000 concluded that the male and female chickens performed similar values of\u0000 serum indices. The contribution of this study might expand the knowledge on\u0000 the biochemical profile and improved breeding strategies of Vietnamese\u0000 indigenous Noi chickens.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134250289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Abdel-Rahman, Y. Mustafa, Hagar Errasool Abd, Hanim S Heikal, A. Elmaghraby
For the association between of Follicle stimulating hormone receptor (FSHR) gene (partial part of exon 10) polymorphisms and litter size trait in Egyptian Ossimi sheep, polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and DNA sequencing techniques were developed. Fifty female Ossimi sheep reared under Egyptian conditions were selected according to their litter size. DNA from blood samples of these animals was isolated to amplify 250-bp of the FSHR gene influencing litter size production trait in sheep. Based on litter size, 50 animals were selected from the highest to the lowest litter size productivity during three seasons. PCR-SSCP analysis of the FSHR gene (250-bp) showed two various genotypes AA and with frequencies 0.64 and 0.36, respectively. The frequencies of the A and B alleles were 0.82 and 0.18, respectively. PCR fragment of FSHR gene (191-bp) was sequenced only in the high and low litter size productivity animals (GenBank accession numbers from MG973191 to MG973207, sequentially). The result indicated that 6SNPs (G/71, G/72, G/77, A/110, A/111, A/191) in high fertile animals, while, 10 SNPs (T/1, C/2, T/14, A/69, A/70, A/71, A/74, G/74, A/75, A/136) have found in low fertile animals. Statistically, AA and genotypes have no significant differences (p> 0.05) on litter size trait in Ossimi sheep. FSHR (exon 10) locus was moderate polymorphic (PIC= 0.25) and it can be used for high litter size productivity in Ossimi sheep as a marker-assisted selection (MAS).
{"title":"FSHR (exon 10) gene polymorphisms and its association with fertility trait in Egyptian Ossimi sheep","authors":"S. Abdel-Rahman, Y. Mustafa, Hagar Errasool Abd, Hanim S Heikal, A. Elmaghraby","doi":"10.2298/BAH1902127A","DOIUrl":"https://doi.org/10.2298/BAH1902127A","url":null,"abstract":"For the association between of Follicle stimulating hormone receptor (FSHR)\u0000 gene (partial part of exon 10) polymorphisms and litter size trait in\u0000 Egyptian Ossimi sheep, polymerase chain reaction-single stranded\u0000 conformational polymorphism (PCR-SSCP) and DNA sequencing techniques were\u0000 developed. Fifty female Ossimi sheep reared under Egyptian conditions were\u0000 selected according to their litter size. DNA from blood samples of these\u0000 animals was isolated to amplify 250-bp of the FSHR gene influencing litter\u0000 size production trait in sheep. Based on litter size, 50 animals were\u0000 selected from the highest to the lowest litter size productivity during\u0000 three seasons. PCR-SSCP analysis of the FSHR gene (250-bp) showed two\u0000 various genotypes AA and with frequencies 0.64 and 0.36, respectively.\u0000 The frequencies of the A and B alleles were 0.82 and 0.18, respectively. PCR\u0000 fragment of FSHR gene (191-bp) was sequenced only in the high and low litter\u0000 size productivity animals (GenBank accession numbers from MG973191 to\u0000 MG973207, sequentially). The result indicated that 6SNPs (G/71, G/72, G/77,\u0000 A/110, A/111, A/191) in high fertile animals, while, 10 SNPs (T/1, C/2,\u0000 T/14, A/69, A/70, A/71, A/74, G/74, A/75, A/136) have found in low fertile\u0000 animals. Statistically, AA and genotypes have no significant differences\u0000 (p> 0.05) on litter size trait in Ossimi sheep. FSHR (exon 10) locus was\u0000 moderate polymorphic (PIC= 0.25) and it can be used for high litter size\u0000 productivity in Ossimi sheep as a marker-assisted selection (MAS).","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129265475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shahin Khan Shah, H. Ali, S. Hayat, Sohail Ahmad, M. Ibrahim, Syed Haider Adnan, I. Ahmad, Haq Ul Ihtesham
Pregnancy recognition in ruminant affects numerous genes regulation. Interferon-stimulatory gene (ISG15), an ubiquitin-like protein that mediates theconjugation of different proteins through its ISGylation enzymes UBE1L and UBCH8, is also differentially expressed during early pregnancy. The purpose of this study was to investigate the role of ISG-15 in the establishment of pregnancy and conceptus elongation during early post conception periods and to ascertain the presence of ISGylation enzymes UBE1L and UBCH8. Therefore, sheep were synchronized through cloprostenol sodium and gonadotrophin releasing hormone-1 and serviced by rams. The blood was collected on the post-mating days 6, 11, 12, 13, 14, 16, 17, 21, 23 and 25. The ISG-15, UBE1L and UBCH8 primers were used to amplify the corresponding transcriptomic region using PCR. Recovery rate of each transcriptomic fragment was compared with the housekeeping gene GAPDH. ISG-15 expression was higher on day 12, contrary to UBE1L were higher on day 6 and UBCH8 on day 21. Furthermore, the ISG-15 is ubiquitin-like protein, mediates UBE1L and UBCH8 enzymes to guard the conceptus against viral pathogenicity during early pregnancy.
{"title":"Expression of isg15 and conjugating enzyme during peri-implanation period in sheep","authors":"Shahin Khan Shah, H. Ali, S. Hayat, Sohail Ahmad, M. Ibrahim, Syed Haider Adnan, I. Ahmad, Haq Ul Ihtesham","doi":"10.2298/bah2002215k","DOIUrl":"https://doi.org/10.2298/bah2002215k","url":null,"abstract":"Pregnancy recognition in ruminant affects numerous genes regulation.\u0000 Interferon-stimulatory gene (ISG15), an ubiquitin-like protein that mediates\u0000 theconjugation of different proteins through its ISGylation enzymes UBE1L\u0000 and UBCH8, is also differentially expressed during early pregnancy. The\u0000 purpose of this study was to investigate the role of ISG-15 in the\u0000 establishment of pregnancy and conceptus elongation during early post\u0000 conception periods and to ascertain the presence of ISGylation enzymes UBE1L\u0000 and UBCH8. Therefore, sheep were synchronized through cloprostenol sodium\u0000 and gonadotrophin releasing hormone-1 and serviced by rams. The blood was\u0000 collected on the post-mating days 6, 11, 12, 13, 14, 16, 17, 21, 23 and 25.\u0000 The ISG-15, UBE1L and UBCH8 primers were used to amplify the corresponding\u0000 transcriptomic region using PCR. Recovery rate of each transcriptomic\u0000 fragment was compared with the housekeeping gene GAPDH. ISG-15 expression\u0000 was higher on day 12, contrary to UBE1L were higher on day 6 and UBCH8 on\u0000 day 21. Furthermore, the ISG-15 is ubiquitin-like protein, mediates UBE1L\u0000 and UBCH8 enzymes to guard the conceptus against viral pathogenicity during\u0000 early pregnancy.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131081121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Petrović, V. Caro-Petrovic, D. Ružić-Muslić, Nevena Maksimović, Vukasin Stefanovic, Bogdan Cekić, I. Ćosić
Quantitative genetic analysis of variability and relationship of lambs body weight traits in indigenous pirot sheep population are done. The examined lambs had high variability which very suitable for selection on a larger weight. There is a high and very significant correlation between the body weight of lambs with 30 and 60 days (.969 **) and the weight with 30 and 90 days (.914 **). There is also a highly significant correlation between the weights of lambs with 60 and 90 days of age (.904 **). From our research, we can see that the first month of the lamb's life is very important for the further development of the body. There is different level of correlation between weight of lambs. This gives us an idea to say that many paragenetic factors are crucial for the growth of lambs from birth to weaning. The coefficient of multiple determination (R2) is 0.845 which means that 8.45% of the lamb's body weight variance at 90 days is determined by the variance of the set of predictor variables (PI-60, 30, 1). Each increase in lamb body weight during the observed periods of age is associated with an increase in the score of depending variable PI90. In particular, any increase in lamb body weight at birth by 1 kg is associated with an increase in lamb body weight from 90 days by 0.238 kg.
{"title":"Quantitative genetic analysis of variability and relationship of lambs body weight traits in population of indigenous Pirot sheep","authors":"M. Petrović, V. Caro-Petrovic, D. Ružić-Muslić, Nevena Maksimović, Vukasin Stefanovic, Bogdan Cekić, I. Ćosić","doi":"10.2298/bah2003271p","DOIUrl":"https://doi.org/10.2298/bah2003271p","url":null,"abstract":"Quantitative genetic analysis of variability and relationship of lambs body\u0000 weight traits in indigenous pirot sheep population are done. The\u0000 examined lambs had high variability which very suitable for selection on a\u0000 larger weight. There is a high and very significant correlation between the\u0000 body weight of lambs with 30 and 60 days (.969 **) and the weight with 30\u0000 and 90 days (.914 **). There is also a highly significant correlation\u0000 between the weights of lambs with 60 and 90 days of age (.904 **). From our\u0000 research, we can see that the first month of the lamb's life is very\u0000 important for the further development of the body. There is different level\u0000 of correlation between weight of lambs. This gives us an idea to say that\u0000 many paragenetic factors are crucial for the growth of lambs from birth to\u0000 weaning. The coefficient of multiple determination (R2) is 0.845 which means\u0000 that 8.45% of the lamb's body weight variance at 90 days is determined by\u0000 the variance of the set of predictor variables (PI-60, 30, 1). Each increase\u0000 in lamb body weight during the observed periods of age is associated with an\u0000 increase in the score of depending variable PI90. In particular, any\u0000 increase in lamb body weight at birth by 1 kg is associated with an increase\u0000 in lamb body weight from 90 days by 0.238 kg.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"127 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115238068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Trbovic, M. Stanković, I. Živić, V. Djordjević, J. Ćirić, M. Velebit, Z. Markovic
The aim of this study was to replace fish meal (FM) with soy protein concentrate (SPC) in carp diets. During a carp feeding trial, the replacement of FM with SPC in four diets 100% replacement (SPC100); 50% replacement (SPC50); 25% replacement (SPC25), and; 0% replacement (SPC0) had no negative effects on the feed conversion ratio or the feed efficiency ratio of the live carp. However, significant differences in the specific growth rate and condition factor were found. The data obtained showed the four different carp diets led to differences in the chemical composition of the resultant carp meat. Between dietary treatments, significant differences were seen in the level of saturated fatty acids (FAs) in carp meat (P <