Pub Date : 2024-12-28DOI: 10.1016/j.bioelechem.2024.108895
Linhong Cao , Tianyu Wang , Jingling Xie , Yihua Wang , Yaxin Huang , Sijian Luo , Xiaoting Zhan , Hui Jiang , Liuxin Ran , Xing Jin , Jinbo Liu , Baolin Li
In this study, an innovative electrochemical biosensor was developed for the rapid, specific, and sensitive detection of Acinetobacter baumannii without the need for sample pretreatment. The biosensor utilized an aptamer as a specific capture probe for A. baumannii and employed a self-powered DNAzyme walker cleavage cycle reaction to achieve signal amplification. Upon introduction of the target bacteria, the aptamer captured the bacteria and released the Trigger, activating the DNAzyme to cleave the substrate chain containing methylene blue (MB). This led to the release of MB-labeled DNA fragments from the electrode surface, resulting in a significant decrease in the square wave voltammetry (SWV) signal of MB on the sensing platform. The limit of detection (LOD) for A. baumannii was determined to be 30 CFU/mL, enabling discrimination of the target bacteria from other common clinical isolates. Furthermore, the biosensor’s potential for real sample analysis was demonstrated in cerebrospinal fluid (CSF), showcasing its efficacy and versatility as a biosensing tool with wide-ranging applications in disease diagnosis and bioanalysis.
{"title":"Aptamer-based DNAzyme walker electrochemical biosensing strategy for Acinetobacter baumannii detection","authors":"Linhong Cao , Tianyu Wang , Jingling Xie , Yihua Wang , Yaxin Huang , Sijian Luo , Xiaoting Zhan , Hui Jiang , Liuxin Ran , Xing Jin , Jinbo Liu , Baolin Li","doi":"10.1016/j.bioelechem.2024.108895","DOIUrl":"10.1016/j.bioelechem.2024.108895","url":null,"abstract":"<div><div>In this study, an innovative electrochemical biosensor was developed for the rapid, specific, and sensitive detection of <em>Acinetobacter baumannii</em> without the need for sample pretreatment. The biosensor utilized an aptamer as a specific capture probe for <em>A. baumannii</em> and employed a self-powered DNAzyme walker cleavage cycle reaction to achieve signal amplification. Upon introduction of the target bacteria, the aptamer captured the bacteria and released the Trigger, activating the DNAzyme to cleave the substrate chain containing methylene blue (MB). This led to the release of MB-labeled DNA fragments from the electrode surface, resulting in a significant decrease in the square wave voltammetry (SWV) signal of MB on the sensing platform. The limit of detection (LOD) for <em>A. baumannii</em> was determined to be 30 CFU/mL, enabling discrimination of the target bacteria from other common clinical isolates. Furthermore, the biosensor’s potential for real sample analysis was demonstrated in cerebrospinal fluid (CSF), showcasing its efficacy and versatility as a biosensing tool with wide-ranging applications in disease diagnosis and bioanalysis.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108895"},"PeriodicalIF":4.8,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Accurate quantification of specific biomarkers is essential for clinical diagnosis and evaluating therapeutic efficacy. A self-signal-amplifying poly(acrylic acid) (PAA)/polyaniline (PANI) film-modified disposable and cost-effective screen-printed carbon electrode (SPCE) has been developed for constructing new label-free immunosensors targeting two model biomarkers: human immunoglobulin G (IgG) and alpha-fetoprotein (AFP). The electrochemically deposited PAA/PANI film on the SPCE serves a dual function: both a bio-immobilization support and a signal amplifier, enhancing biomarker detection sensitivity and efficiency. The self-signal amplification properties of PANI streamline the detection process. At the same time, the high-density surface carboxyl groups from embedded PAA enable covalent conjugation with capture antibodies (anti-IgG and anti-AFP). Subsequently, antibody-immobilized PAA/PANI film-modified SPCEs, as immunosensors, successfully detect IgG and AFP without the need for external redox probes. The reductions in the electrochemical PANI signals of the immunosensors are linearly proportional to the logarithm of IgG and AFP concentrations. The proposed immunosensors exhibit sufficiently wide ranges of calibration curves from 0.10 to 50 ng mL−1, with limits of detection of 0.080 ng mL−1 for IgG and 0.090 ng mL−1 for AFP. The sensors exhibit satisfactory sensitivity and selectivity, indicating their potential for accurate and reliable detection.
特异性生物标志物的准确定量对临床诊断和评估治疗效果至关重要。制备了一种自信号放大的聚丙烯酸(PAA)/聚苯胺（PANI）薄膜修饰的一次性丝网印刷碳电极（SPCE），用于构建新的无标记免疫传感器，该电极针对两种模型生物标志物：人免疫球蛋白G （IgG）和甲胎蛋白（AFP）。电化学沉积在SPCE上的PAA/PANI膜具有双重功能：生物固定支架和信号放大器，提高了生物标志物检测的灵敏度和效率。聚苯胺的自信号放大特性简化了检测过程。同时，包埋PAA的高密度表面羧基使其能够与捕获抗体（抗igg和抗afp）共价偶联。随后，固定化PAA/PANI膜修饰的spce作为免疫传感器，成功检测IgG和AFP，而无需外部氧化还原探针。免疫传感器电化学PANI信号的减少与IgG和AFP浓度的对数成线性比例。所提出的免疫传感器具有足够宽的校准曲线范围，从0.10到50 ng mL-1， IgG的检测限为0.080 ng mL-1， AFP的检测限为0.090 ng mL-1。传感器表现出令人满意的灵敏度和选择性，表明它们具有准确可靠检测的潜力。
{"title":"Leveraging self-signal amplifying poly(acrylic acid)/polyaniline electrodes for label-free electrochemical immunoassays in protein biomarker detection","authors":"Supakeit Chanarsa , Sopit Phetsang , Wiradej Thongsuwan , Thunwadee Limtharakul , Jidapha Tinoi , Jaroon Jakmunee , Kontad Ounnunkad","doi":"10.1016/j.bioelechem.2024.108894","DOIUrl":"10.1016/j.bioelechem.2024.108894","url":null,"abstract":"<div><div>Accurate quantification of specific biomarkers is essential for clinical diagnosis and evaluating therapeutic efficacy. A self-signal-amplifying poly(acrylic acid) (PAA)/polyaniline (PANI) film-modified disposable and cost-effective screen-printed carbon electrode (SPCE) has been developed for constructing new label-free immunosensors targeting two model biomarkers: human immunoglobulin G (IgG) and alpha-fetoprotein (AFP). The electrochemically deposited PAA/PANI film on the SPCE serves a dual function: both a bio-immobilization support and a signal amplifier, enhancing biomarker detection sensitivity and efficiency. The self-signal amplification properties of PANI streamline the detection process. At the same time, the high-density surface carboxyl groups from embedded PAA enable covalent conjugation with capture antibodies (anti-IgG and anti-AFP). Subsequently, antibody-immobilized PAA/PANI film-modified SPCEs, as immunosensors, successfully detect IgG and AFP without the need for external redox probes. The reductions in the electrochemical PANI signals of the immunosensors are linearly proportional to the logarithm of IgG and AFP concentrations. The proposed immunosensors exhibit sufficiently wide ranges of calibration curves from 0.10 to 50 ng mL<sup>−1</sup>, with limits of detection of 0.080 ng mL<sup>−1</sup> for IgG and 0.090 ng mL<sup>−1</sup> for AFP. The sensors exhibit satisfactory sensitivity and selectivity, indicating their potential for accurate and reliable detection.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108894"},"PeriodicalIF":4.8,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-27DOI: 10.1016/j.bioelechem.2024.108893
Sora Lee , Myung-Min Oh
Numerous studies in various species have demonstrated that the application of an electric field can improve plant growth. However, plants showed inconsistent responses and the background mechanism for responses to electric fields remain unclear. Here, to deepen our understanding of the mechanisms involved in electric field–induced changes in physiology, we investigated the effects of electric fields on the growth and development of Arabidopsis (Arabidopsis thaliana). To this end, we cultivated Arabidopsis plants under 5 kV/m electric fields oriented vertically or horizontally. Regardless of the electric field direction, the exposed plants had significantly more leaves and greater biomass compared to the control group, which was not exposed to an additional electric field. Exposure to the electric fields also accelerated flowering. Auxin concentration in Arabidopsis leaves exhibited significant increase following exposure to electric field, supporting the enhanced shoot growth. Moreover, well-developed xylem and phloem under vertical electric fields facilitated increased absorption of water and nutrients. Gene ontology annotation and KEGG pathway analysis identified numerous electric field–responsive genes. Overall, this study elucidates mechanisms of the plant response to electric fields and represents a step towards developing technologies that enhance crop productivity.
{"title":"Electrocultivation of Arabidopsis thaliana increases water and mineral absorption, electric charge and auxin accumulation, enhancing growth and development","authors":"Sora Lee , Myung-Min Oh","doi":"10.1016/j.bioelechem.2024.108893","DOIUrl":"10.1016/j.bioelechem.2024.108893","url":null,"abstract":"<div><div>Numerous studies in various species have demonstrated that the application of an electric field can improve plant growth. However, plants showed inconsistent responses and the background mechanism for responses to electric fields remain unclear. Here, to deepen our understanding of the mechanisms involved in electric field–induced changes in physiology, we investigated the effects of electric fields on the growth and development of Arabidopsis (<em>Arabidopsis thaliana</em>). To this end, we cultivated Arabidopsis plants under 5 kV/m electric fields oriented vertically or horizontally. Regardless of the electric field direction, the exposed plants had significantly more leaves and greater biomass compared to the control group, which was not exposed to an additional electric field. Exposure to the electric fields also accelerated flowering. Auxin concentration in Arabidopsis leaves exhibited significant increase following exposure to electric field, supporting the enhanced shoot growth. Moreover, well-developed xylem and phloem under vertical electric fields facilitated increased absorption of water and nutrients. Gene ontology annotation and KEGG pathway analysis identified numerous electric field–responsive genes. Overall, this study elucidates mechanisms of the plant response to electric fields and represents a step towards developing technologies that enhance crop productivity.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108893"},"PeriodicalIF":4.8,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-26DOI: 10.1016/j.bioelechem.2024.108888
Yuan Ming , Yujie Liu , Daxiu Li , Bingying Jiang , Yun Xiang , Ruo Yuan
The concentration variation of luteinizing hormone (LH) regulates the cell cycle of oocyte meiosis and significantly affect the whole reproductive cycle. Sensitively quantifying the LH biomarker therefore plays an important role for reproductive disease diagnosis. By coupling a new low background catalytic redox recycling strategy with hybridization chain reaction (HCR), we propose a highly sensitive bio-electrochemical aptamer LH sensing method. LH analyte molecules bind aptamer strands in duplex DNAs to liberate ssDNAs, which trigger HCR generation of [Ru(NH3)6]Cl3 (RuHex)-modified dsDNA polymers on sensor electrode. Subsequent electrochemical redox recycling of RuHex mediated by K3[Fe(CN)6] thus exhibits greatly magnified currents for ultrasensitive LH assay. The synergistic integration of HCR signal amplification with low background redox recycling leads to highly enhanced signal-to-noise ratio and sensitivity for detecting LH down to 6.03 pM. In addition, LH sensing in diluted human serums has been tested and verified, making such sensor a robust detection platform for monitoring diverse biomarkers at low levels for early diagnosing diseases.
{"title":"Low background catalytic redox recycling coupled with hybridization chain reaction amplification for highly sensitive electrochemical aptamer luteinizing hormone assay","authors":"Yuan Ming , Yujie Liu , Daxiu Li , Bingying Jiang , Yun Xiang , Ruo Yuan","doi":"10.1016/j.bioelechem.2024.108888","DOIUrl":"10.1016/j.bioelechem.2024.108888","url":null,"abstract":"<div><div>The concentration variation of luteinizing hormone (LH) regulates the cell cycle of oocyte meiosis and significantly affect the whole reproductive cycle. Sensitively quantifying the LH biomarker therefore plays an important role for reproductive disease diagnosis. By coupling a new low background catalytic redox recycling strategy with hybridization chain reaction (HCR), we propose a highly sensitive bio-electrochemical aptamer LH sensing method. LH analyte molecules bind aptamer strands in duplex DNAs to liberate ssDNAs, which trigger HCR generation of [Ru(NH<sub>3</sub>)<sub>6</sub>]Cl<sub>3</sub> (RuHex)-modified dsDNA polymers on sensor electrode. Subsequent electrochemical redox recycling of RuHex mediated by K<sub>3</sub>[Fe(CN)<sub>6</sub>] thus exhibits greatly magnified currents for ultrasensitive LH assay. The synergistic integration of HCR signal amplification with low background redox recycling leads to highly enhanced signal-to-noise ratio and sensitivity for detecting LH down to 6.03 pM. In addition, LH sensing in diluted human serums has been tested and verified, making such sensor a robust detection platform for monitoring diverse biomarkers at low levels for early diagnosing diseases.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108888"},"PeriodicalIF":4.8,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-26DOI: 10.1016/j.bioelechem.2024.108891
Haitang Yang , Siming Pu , Penghua Shu , Jiapan Wang , YuYu Chen , Xinshuo Yang , Yuce Hou , Wei Wei
Early diagnosis of tumors is becoming increasingly important in modern healthcare. As studies have demonstrated, Poly(ADP)ribose polymerase-1 (PARP-1) is overexpressed in more aggressive tumors. Consequently, sensitive detection of PARP-1 activity holds significant practical importance in clinical diagnostics and biomedical research. Herein, an electrochemical biosensor for the sensitive monitoring of the PARP-1 activity have been proposed. The presence of target PARP-1 firstly triggers enzyme-initiated auto-PARylation and formed negatively charged polymer consisting of a few to 200 ADP-ribose units. Due to electrostatic adsorption, negatively charged PAR will bind with a large number of positively charged methylene blue (MB) electroactive molecules. By detecting the electrochemical signal of MB on the indium tin oxide (ITO) electrode, PARP-1 activity detection was achieved with a linear detection range of 0–1.0 U and a detection limit as low as 0.003 U. The proposed biosensor shows great prospects of clinical application.
{"title":"A label-free electrochemical biosensor for sensitive analysis of the PARP-1 activity","authors":"Haitang Yang , Siming Pu , Penghua Shu , Jiapan Wang , YuYu Chen , Xinshuo Yang , Yuce Hou , Wei Wei","doi":"10.1016/j.bioelechem.2024.108891","DOIUrl":"10.1016/j.bioelechem.2024.108891","url":null,"abstract":"<div><div>Early diagnosis of tumors is becoming increasingly important in modern healthcare. <u>As studies have demonstrated, Poly(ADP)ribose polymerase-1 (PARP-1) is overexpressed in more aggressive tumors. Consequently, sensitive detection of PARP-1 activity holds significant practical importance in clinical diagnostics and biomedical research</u>. Herein, an electrochemical biosensor for the sensitive monitoring of the PARP-1 activity have been proposed. The presence of target PARP-1 firstly triggers enzyme-initiated auto-PARylation and formed negatively charged polymer consisting of a few to 200 ADP-ribose units. Due to electrostatic adsorption, negatively charged PAR will bind with a large number of positively charged methylene blue (MB) electroactive molecules. By detecting the electrochemical signal of MB on the <u>indium tin oxide (ITO)</u> electrode, PARP-1 activity detection was achieved with a linear detection range of 0–1.0 U and a detection limit as low as 0.003 U. The proposed biosensor shows great prospects of clinical application.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108891"},"PeriodicalIF":4.8,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study investigates the corrosion of 90/10 copper-nickel (Cu-Ni) alloy caused by sulfate-reducing bacteria (SRB) in the presence of aluminum anodes, with particular emphasis on the role of electron supply in microbial corrosion and the resulting local corrosion failures. The study reveals that the electron supply from the anode supports SRB growth on the Cu-Ni alloy through an “Electrons-siphoning” mechanism. However, the supply is insufficient to sustain the SRB population, resulting in ineffective cathodic protection (icorr = 2.34 × 10−6 A cm−2). The addition of 20 ppm riboflavin (RF) to the SRB biofilm enhances electrical activity and increases the electron donor density, thereby restoring the anode’s protective effect. As a result, the icorr of the 90/10 Cu-Ni alloy decreases by an order of magnitude (to 3.5 × 10−7 A cm−2). These findings provide valuable new insights into the mechanisms of microbial corrosion.
本研究研究了在铝阳极存在的情况下，硫酸盐还原菌（SRB）对90/10铜镍（Cu-Ni）合金的腐蚀，特别强调了电子供应在微生物腐蚀中的作用以及由此导致的局部腐蚀失效。研究表明，阳极的电子供给通过“电子虹吸”机制支持SRB在Cu-Ni合金上的生长。然而，供应不足以维持SRB种群，导致无效的阴极保护（icorr = 2.34 × 10-6 A cm-2）。在SRB生物膜中加入20ppm的核黄素（RF）可以增强电活性，增加电子供体密度，从而恢复阳极的保护作用。因此，90/10 Cu-Ni合金的icorr降低了一个数量级（为3.5 × 10-7 a cm-2）。这些发现为微生物腐蚀的机理提供了有价值的新见解。
{"title":"Sulfate reducing bacteria corrosion of a 90/10 Cu-Ni alloy coupled to an Al sacrificial anode","authors":"Huixuan Qian , Tianguan Wang , Peng Xu , Zhiyuan Feng , Bing Lei , Ping Zhang , Honglei Guo , Guozhe Meng","doi":"10.1016/j.bioelechem.2024.108892","DOIUrl":"10.1016/j.bioelechem.2024.108892","url":null,"abstract":"<div><div>This study investigates the corrosion of 90/10 copper-nickel (Cu-Ni) alloy caused by sulfate-reducing bacteria (SRB) in the presence of aluminum anodes, with particular emphasis on the role of electron supply in microbial corrosion and the resulting local corrosion failures. The study reveals that the electron supply from the anode supports SRB growth on the Cu-Ni alloy through an “Electrons-siphoning” mechanism. However, the supply is insufficient to sustain the SRB population, resulting in ineffective cathodic protection (<em>i</em><sub>corr</sub> = 2.34 × 10<sup>−6</sup> A cm<sup>−2</sup>). The addition of 20 ppm riboflavin (RF) to the SRB biofilm enhances electrical activity and increases the electron donor density, thereby restoring the anode’s protective effect. As a result, the <em>i</em><sub>corr</sub> of the 90/10 Cu-Ni alloy decreases by an order of magnitude (to 3.5 × 10<sup>−7</sup> A cm<sup>−2</sup>). These findings provide valuable new insights into the mechanisms of microbial corrosion.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108892"},"PeriodicalIF":4.8,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-25DOI: 10.1016/j.bioelechem.2024.108889
Xiaojun Liu , Huihui Dong , Qinyu Wang , Jing Yang , Xinru Zhai , Mingzhen Lin , Kaixin Liu , Qinzheng Yang
Sediment microbial fuel cell (SMFC) is a device for biological denitrification, in which electrons produced by sediment microorganisms can be transferred to the upper layer of the water column lacking electron donors. However, the low efficiency of denitrifying bacteria in acquiring electrons and enriching at the cathode greatly hinders the application of SMFC for nitrogen removal. In this study, we report a novel method of constructing a high-performance biocathode by modifying electrodes with zero-valent iron to enhance the enrichment and electron transfer of electroactive bacteria. The surface chemical and biological analysis of the biocathode revealed that the ZVI gradually oxidized to form magnetite and goethite, and finally stabilized into better crystallized lepidocrocite. On the other hand, the microbial community of the biocathode gradually evolved into a community dominated by denitrifying bacteria, specifically Clostridium. The co-evolved “Clostridium-lepidocrocite” composite endows the sediment microbial fuel cell with a 99% nitrate removal capacity. These results indicate that the cathode constructed by using ZVI modified electrode achieves efficient nitrate reduction by denitrifying bacteria. Furthermore, the construction method of biocathode may also have the potential application in water remediation and the geochemical cycling of elements.
{"title":"Efficient nitrate removal via microorganism-iron oxide co-evolution on biocathode surface","authors":"Xiaojun Liu , Huihui Dong , Qinyu Wang , Jing Yang , Xinru Zhai , Mingzhen Lin , Kaixin Liu , Qinzheng Yang","doi":"10.1016/j.bioelechem.2024.108889","DOIUrl":"10.1016/j.bioelechem.2024.108889","url":null,"abstract":"<div><div>Sediment microbial fuel cell (SMFC) is a device for biological denitrification, in which electrons produced by sediment microorganisms can be transferred to the upper layer of the water column lacking electron donors. However, the low efficiency of denitrifying bacteria in acquiring electrons and enriching at the cathode greatly hinders the application of SMFC for nitrogen removal. In this study, we report a novel method of constructing a high-performance biocathode by modifying electrodes with zero-valent iron to enhance the enrichment and electron transfer of electroactive bacteria. The surface chemical and biological analysis of the biocathode revealed that the ZVI gradually oxidized to form magnetite and goethite, and finally stabilized into better crystallized lepidocrocite. On the other hand, the microbial community of the biocathode gradually evolved into a community dominated by denitrifying bacteria, specifically <em>Clostridium</em>. The co-evolved “<em>Clostridium</em>-lepidocrocite” composite endows the sediment microbial fuel cell with a 99% nitrate removal capacity. These results indicate that the cathode constructed by using ZVI modified electrode achieves efficient nitrate reduction by denitrifying bacteria. Furthermore, the construction method of biocathode may also have the potential application in water remediation and the geochemical cycling of elements.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108889"},"PeriodicalIF":4.8,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-24DOI: 10.1016/j.bioelechem.2024.108885
Lu Yang , Yang Wu , Songpei Hu , Jiafeng Yao , Fangming Chen
An electrical characteristics extraction and analysis method for membrane of medaka embryo during its development using Electrical Impedance Spectroscopy (EIS) is proposed. The proposed method is non-invasive, it doesn’t affect the embryo’s development. Embryo’s equivalent electrical circuit (EEC) model is established to extract membrane’s electrical characteristics, it is used to fit the embryo’s electrical impedance curves in different developmental stages. The equivalent resistances and capacitances of membrane are regarded as the electrical characteristics to analyze the membrane’s physiological changes during embryo’s development. To achieve the embryo’s electrical impedance curve from the measurement system exactly, an EIT-assisted electrical impedance curve extraction method is innovatively introduced. The extracted electrical characteristics of the membrane reflect its physiological changes well in both simulation and experiment. In experiment, the equivalent capacitances of embryo’s membrane are increasing, while the equivalent resistances show a downward trend from neurula stage to hatching stage. The experiment results indicate that the permeability of membrane becomes higher and the thickness of membrane becomes thinner from neurula stage to hatching stage.
{"title":"An electrical characteristics extraction and analysis method for the membrane of medaka embryo during its development using electrical impedance spectroscopy","authors":"Lu Yang , Yang Wu , Songpei Hu , Jiafeng Yao , Fangming Chen","doi":"10.1016/j.bioelechem.2024.108885","DOIUrl":"10.1016/j.bioelechem.2024.108885","url":null,"abstract":"<div><div>An electrical characteristics extraction and analysis method for membrane of medaka embryo during its development using Electrical Impedance Spectroscopy (EIS) is proposed. The proposed method is non-invasive, it doesn’t affect the embryo’s development. Embryo’s equivalent electrical circuit (EEC) model is established to extract membrane’s electrical characteristics, it is used to fit the embryo’s electrical impedance curves in different developmental stages. The equivalent resistances and capacitances of membrane are regarded as the electrical characteristics to analyze the membrane’s physiological changes during embryo’s development. To achieve the embryo’s electrical impedance curve from the measurement system exactly, an EIT-assisted electrical impedance curve extraction method is innovatively introduced. The extracted electrical characteristics of the membrane reflect its physiological changes well in both simulation and experiment. In experiment, the equivalent capacitances of embryo’s membrane are increasing, while the equivalent resistances show a downward trend from neurula stage to hatching stage. The experiment results indicate that the permeability of membrane becomes higher and the thickness of membrane becomes thinner from neurula stage to hatching stage.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108885"},"PeriodicalIF":4.8,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bismuth tungstate perovskite has been identified as a promising photoelectric material. Nevertheless, the wide band gap of bismuth tungstate leads to short-wavelength absorption of a single material with an attenuated photocurrent response, hindering its realization in biosensing applications. In this study, F, S co-doped Bi2WO6 was synthesized by heat treatment and combined with SnS2 and CdS to form a ternary heterojunction composite. The resulting composite material, marked as F, S-Bi2WO6@SnS2@CdS, has excellent photoelectric characteristics. F, S co-doping can increase the number of oxygen vacancies, effectively reducing the band gap, and the introduction of narrow band gap metal-sulfur compounds can form ternary heterojunctions with them, further red-shifting the optical absorption wavelength, while greatly improving the photocurrent response through good energy level matching. The excellent level matching between AgInS2 and F,S-Bi2WO6@SnS2@CdS results in photocurrent enhancement. The competition between AgInS2-Ab-TB and AgInS2-Ab for limited binding sites leads to changes in the photocurrent signal, which can sensibly detect TB. The prepared PEC biosensor has excellent photocurrent response in the range of 0.1 pg/mL − 100 ng/mL, and the detection limit is 28.9 fg/mL. This study broadens the application of bismuth tungstate chalcogenide in biosensing and provides new ideas for the modification of other optoelectronic materials.
{"title":"Enhancement of bismuth tungstate perovskite photoelectrical performance using elemental co-doping and construction of ternary heterojunction for sensitive detection of Trenbolone","authors":"Yanan Wu, Weixuan Xu, Feng Jiang, Shanghua Liu, Yueyuan Li, Shujun Wang, Zhen Xu, Yueyun Li","doi":"10.1016/j.bioelechem.2024.108887","DOIUrl":"10.1016/j.bioelechem.2024.108887","url":null,"abstract":"<div><div>Bismuth tungstate perovskite has been identified as a promising photoelectric material. Nevertheless, the wide band gap of bismuth tungstate leads to short-wavelength absorption of a single material with an attenuated photocurrent response, hindering its realization in biosensing applications. In this study, F, S co-doped Bi<sub>2</sub>WO<sub>6</sub> was synthesized by heat treatment and combined with SnS<sub>2</sub> and CdS to form a ternary heterojunction composite. The resulting composite material, marked as F, S-Bi<sub>2</sub>WO<sub>6</sub>@SnS<sub>2</sub>@CdS, has excellent photoelectric characteristics. F, S co-doping can increase the number of oxygen vacancies, effectively reducing the band gap, and the introduction of narrow band gap metal-sulfur compounds can form ternary heterojunctions with them, further red-shifting the optical absorption wavelength, while greatly improving the photocurrent response through good energy level matching. The excellent level matching between AgInS<sub>2</sub> and F,S-Bi<sub>2</sub>WO<sub>6</sub>@SnS<sub>2</sub>@CdS results in photocurrent enhancement. The competition between AgInS<sub>2</sub>-Ab-TB and AgInS<sub>2</sub>-Ab for limited binding sites leads to changes in the photocurrent signal, which can sensibly detect TB. The prepared PEC biosensor has excellent photocurrent response in the range of 0.1 pg/mL − 100 ng/mL, and the detection limit is 28.9 fg/mL. This study broadens the application of bismuth tungstate chalcogenide in biosensing and provides new ideas for the modification of other optoelectronic materials.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108887"},"PeriodicalIF":4.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this investigation, a novel tetradentate Schiff base ligand, (ligand L) was synthesized using a simple chemical route assisted by triethylenetetramine with 4-dimethylaminocinnamaldehyde in ethanol. The chemical structure of the as-synthesized ligand was characterized using nuclear magnetic resonance (NMR) and UV–visible spectroscopy. This ligand was then employed to modify the working electrode of screen-printed carbon electrode (SPCE) for developing a modified L/SPCE sensor finalized to detection of lead ions (Pb2+). The electrochemical characteristics of the sensor were assessed by Square Wave Anodic Stripping Voltammetry technique (SWASV). To further enhance the sensitivity, gold nanoparticles (AuNps) were deposited on the surface of the working electrode for obtaining an AuNps-L/SPCE sensor. This device shows a linear response to Pb2+ until to 0.6 µM, a sensitivity of 897 µA µM−1 cm−2 and a limit of detection (LOD) of 0.38 μM. This successful strategy offers promising avenues for lead ion detection also in urine.
本研究以四乙基四胺和4-二甲氨基肉桂醛为辅助剂，在乙醇中合成了一种新型的四齿席夫碱配体（配体L）。利用核磁共振（NMR）和紫外可见光谱对合成配体的化学结构进行了表征。然后利用该配体修饰丝网印刷碳电极（SPCE）的工作电极，开发出用于检测铅离子（Pb2+）的改性L/SPCE传感器。采用方波阳极溶出伏安法（SWASV）对传感器的电化学特性进行了评价。为了进一步提高灵敏度，在工作电极表面沉积了金纳米粒子（AuNps），获得了AuNps- l /SPCE传感器。该器件对Pb2+的线性响应范围为0.6µM，灵敏度为897µaµM-1 cm-2，检测限（LOD）为0.38 μM。这一成功的策略也为尿液中的铅离子检测提供了有希望的途径。
{"title":"Novel gold nanoparticles-Schiff base electrochemical sensor for the determination of lead (II) ions in biological samples","authors":"Zahra Akbari , Khouloud Abid , Daniela Iannazzo , Morteza Montazerozohori , Giovanni Neri","doi":"10.1016/j.bioelechem.2024.108886","DOIUrl":"10.1016/j.bioelechem.2024.108886","url":null,"abstract":"<div><div>In this investigation, a novel tetradentate Schiff base ligand, (ligand L) was synthesized using a simple chemical route assisted by triethylenetetramine with 4-dimethylaminocinnamaldehyde in ethanol. The chemical structure of the as-synthesized ligand was characterized using nuclear magnetic resonance (NMR) and UV–visible spectroscopy. This ligand was then employed to modify the working electrode of screen-printed carbon electrode (SPCE) for developing a modified L/SPCE sensor finalized to detection of lead ions (Pb<sup>2+</sup>). The electrochemical characteristics of the sensor were assessed by Square Wave Anodic Stripping Voltammetry technique (SWASV). To further enhance the sensitivity, gold nanoparticles (AuNps) were deposited on the surface of the working electrode for obtaining an AuNps-L/SPCE sensor. This device shows a linear response to Pb<sup>2+</sup> until to 0.6 µM, a sensitivity of 897 µA µM<sup>−1</sup> cm<sup>−2</sup> and a limit of detection (LOD) of 0.38 μM. This successful strategy offers promising avenues for lead ion detection also in urine.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108886"},"PeriodicalIF":4.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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