Bioelectrochemistry最新文献_第7页

Investigations of potential therapeutic targets from high-throughput, label-free, quantitative proteomic studies in MDA-MB-468 cells treated with metformin and electrical pulses 二甲双胍和电脉冲处理MDA-MB-468细胞的高通量、无标记、定量蛋白质组学研究的潜在治疗靶点研究。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-10-24 DOI: 10.1016/j.bioelechem.2025.109153

Praveen Sahu , Ignacio G. Camarillo , Raji Sundararajan

High-throughput, label-free quantitative proteomic analyses, along with cell viability and reactive oxygen species (ROS) studies, were performed on MDA-MB-468 human triple-negative breast cancer (TNBC) cells, to gain mechanistic insights into therapeutic actions. TNBC was chosen as it is the most lethal subtype of breast cancer, highly aggressive, prone to recurrence and metastasis, with the highest prevalence in black women. The absence of specific biomarkers limits targeted therapies. This unmet need was studied using proteomics, after treating the cells with electrical pulses (EP) combined with metformin (met). With the EP + met treatment (1000 V/cm, 1 mM), cell viability dropped to 25.6 % after 24 h and ROS increased to 179 %, compared to control at 100 %. Proteomics revealed 125 upregulated and 37 downregulated proteins in EP + met, compared to met alone, involving enzymes, proliferation markers, and kinases. Key gene changes included upregulation of ALAD, MKI67, and LRCH4, and downregulation of EIF1AX, NSUN5, and GNS. LRCH4 and GNS are suggested to be potential novel therapeutic targets, as LRCH4 upregulation links to inhibition of the mTOR/PI3K/Akt pathway, reducing proliferation, while GNS downregulation suppresses tumor growth and metastasis. Overall, proteomics-based preliminary findings suggest that EP + met modulate TNBC pathways, identifying potential biomarkers and providing a foundation for future validation

在MDA-MB-468人三阴性乳腺癌（TNBC）细胞上进行了高通量、无标记的定量蛋白质组学分析，以及细胞活力和活性氧（ROS）研究，以获得治疗作用的机制见解。选择TNBC是因为它是乳腺癌中最致命的亚型，具有高度侵袭性，容易复发和转移，在黑人妇女中发病率最高。特异性生物标志物的缺乏限制了靶向治疗。在电脉冲（EP）联合二甲双胍（met）处理细胞后，使用蛋白质组学研究了这种未满足的需求。EP + met （1000 V/cm, 1 mM）处理24 h后，细胞活力下降到25.6%，ROS增加到179%，而对照组为100%。蛋白质组学显示，与单独使用EP + met相比，EP + met中有125个蛋白上调，37个蛋白下调，涉及酶、增殖标志物和激酶。关键基因变化包括ALAD、MKI67和LRCH4上调，EIF1AX、NSUN5和GNS下调。LRCH4和GNS被认为是潜在的新治疗靶点，因为LRCH4上调与抑制mTOR/PI3K/Akt通路有关，减少增殖，而GNS下调抑制肿瘤生长和转移。总的来说，基于蛋白质组学的初步研究结果表明，EP + met调节TNBC通路，识别潜在的生物标志物，并为未来的验证提供基础。

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引用次数: 0

Mediated electron transfer in five prevalent human oral microbial species 五种流行的人类口腔微生物物种介导的电子转移。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-10-24 DOI: 10.1016/j.bioelechem.2025.109149

David Hernández-Villamor , Aya Jeaidi , Riet Boydens , Korneel Rabaey , Tom Van de Wiele , Antonin Prévoteau

An increasing number of microbial species within the human body, many of which are pathogenic, are being reported as “electroactive”. However, the mechanisms and kinetics of extracellular electron transfer (EET) and its putative ecological relevance remain understudied. We utilized rotating disk electrodes (RDEs) to assess mediated electron transfer (MET) in five oral species via their ability to reduce riboflavin and ferricyanide. The use of both mediators was confirmed in Streptococcus mutans, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis, while A. viscosus only reduced riboflavin. Kinetics of EET (turnover rate per cell) were slow with riboflavin (k_{cat, RF}

<

10⁴ s⁻¹) in all species but F. nucleatum, whereas ferricyanide resulted in fast kinetics (k_{cat, Ferri(app)}

>

10⁴ s⁻¹) in all but S. mutans. Due to its central role in oral biofilms and association to systemic diseases, MET was further characterized in F. nucleatum. Apparent Michaelis–Menten kinetics showed K_m values of (0.57 ± 0.16 and 10.43 ± 0.91)

μ

M for ferricyanide and riboflavin. The presence of mediators enhanced acetate production compared to mediator-free controls; when ferricyanide was used, butyrate and formate production was triggered only after its depletion. Finally, the putative molecular mechanisms enabling MET in F. nucleatum are discussed.

据报道，人体内越来越多的微生物物种具有“电活性”，其中许多是致病性的。然而，细胞外电子转移（EET）的机制和动力学及其假定的生态相关性仍未得到充分研究。我们利用旋转圆盘电极（RDEs）通过其减少核黄素和铁氰化物的能力来评估五种口腔物种的介导电子转移（MET）。在变形链球菌、核梭杆菌、放线菌聚集杆菌和牙龈卟啉单胞菌中证实了这两种介质的作用，而粘杆菌仅能降低核黄素。核黄素（kcat， RF< 104 s-1）对所有菌种的EET（每个细胞的周转率）的影响较慢，而铁氰化物（kcat， RF< 104 s-1）对所有菌种的EET（每个细胞的周转率）的影响较快，但对变形链球菌的影响较小。由于其在口腔生物膜中的核心作用和与全身性疾病的关联，MET在具核梭菌中得到了进一步的表征。表观Michaelis-Menten动力学表明，铁氰化物和核黄素的Km值分别为（0.57±0.16和10.43±0.91）μM。与无介质对照相比，介质的存在提高了乙酸酯的产量；当使用铁氰化物时，只有在耗尽后才会触发丁酸盐和甲酸盐的生产。最后，讨论了可能的分子机制，使MET在F. nucleatum。

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引用次数: 0

Development of peroxidase-modified zeolite carbon paste electrodes (Prx-Zeo/CPE) for the biosensing of hydroquinone in pharmaceutical skin cream 过氧化物酶修饰的沸石碳糊电极（Prx-Zeo/CPE）在药用护肤霜中对苯二酚的生物传感研究。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-10-22 DOI: 10.1016/j.bioelechem.2025.109150

Roberta C. Martins , Pedro A. da Silva Pereira , Alexandre L.B. Baccaro , Lúcio Angnes , Adelir A. Saczk , Fabiana S. Felix

Peroxidases are oxidoreductase enzymes with intense catalytic activity toward the peroxidation of hydrogen donors in the presence of hydrogen peroxide. These enzymes are appealing for application as the biomolecular recognition step of electrochemical biosensors, since they can be easily extracted from vegetable tissues such as Solanum aethiopicum (popularly known as “Gilo”), considerably reducing the costs of sensing devices. Meanwhile, zeolites are hydrated aluminosilicates arranged in 3D tetrahedral crystalline reticules that form microporous inner structures with high internal and external surface areas, very convenient for the immobilization of high amounts of enzymes to possibly improve the electrochemical biosensors sensitivity. In this study, a peroxidase biosensor utilizing enzymes extracted from raw Gilo tissue was developed. The high adsorption capacity of zeolites (MFI-based NaY) was explored to immobilize the peroxidase enzymes. The extraction efficiency was evaluated using three different types of polymeric protectors: polyvinylpyrrolidone (PVP K90), latex, or polyvinyl alcohol (PVOH. Among them, PVOH was identified as the most effective protector. The peroxidase enzyme was immobilized on NaY zeolite, forming the peroxidase-modified zeolite that was added to carbon paste electrodes to assemble the Prx-Zeo/CPE bioelectrode. The effects of graphite powder, zeolite, mineral oil, and peroxidase concentration in the paste composition were investigated using factorial planning with the premises of the Design of Experiments (DOE) to optimize cathodic current values for hydroquinone detection. Finally, the Prx-Zeo/CPE biosensor was applied to determine the hydroquinone content in two different of skin cream samples. Hydroquinone were determined by direct interpolation of the sample analytical signals onto an analytical curve (0.2–1.0 mmol L⁻¹) and subsequently compared with the label nominal values provided by the manufacturers, as well as with those obtained through parallel analysis using an HPLC reference method.

过氧化物酶是一种氧化还原酶，在过氧化氢存在下对供体氢的过氧化具有强烈的催化活性。这些酶作为电化学生物传感器的生物分子识别步骤很有吸引力，因为它们可以很容易地从植物组织中提取，如埃塞俄比亚茄（俗称“Gilo”），大大降低了传感设备的成本。同时，沸石是水合铝硅酸盐，排列在三维四面体网状晶体中，形成具有高内外表面积的微孔内部结构，非常便于固定化大量酶，可能提高电化学生物传感器的灵敏度。在本研究中，利用从原始Gilo组织中提取的酶开发了过氧化物酶生物传感器。利用高吸附量的沸石（mfi基NaY）固定化过氧化物酶。采用三种不同类型的聚合物保护剂：聚乙烯吡咯烷酮（PVP K90）、乳胶或聚乙烯醇（PVOH）来评估提取效率。其中，PVOH被认为是最有效的保护剂。将过氧化物酶固定在NaY沸石上，形成过氧化物酶修饰的沸石，并将其添加到碳糊电极上组装Prx-Zeo/CPE生物电极。以实验设计（DOE）为前提，研究了石墨粉、沸石、矿物油和过氧化物酶浓度对膏体组成的影响，以优化对苯二酚检测的阴极电流值。最后，应用Prx-Zeo/CPE生物传感器测定两种不同护肤霜样品中对苯二酚的含量。将样品的分析信号直接插值到0.2 ~ 1.0 mmol L-1的分析曲线上，测定对苯二酚的含量，并与生产商提供的标签标称值进行比较，并与HPLC参考法平行分析得到的对苯二酚含量进行比较。

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引用次数: 0

Bio-cathodic treatment of toluene and nutrients in an integrated bubble column-microbial fuel cell: Electricity generation and microbial community shift 集成泡柱-微生物燃料电池中甲苯和营养物的生物阴极处理：发电和微生物群落转移

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-10-22 DOI: 10.1016/j.bioelechem.2025.109148

Raoof Rabiee , Seyed Morteza Zamir , Niusha Safari , Mohammad Ramezani , Mahsa Sedighi

An integrated bubble column reactor–microbial fuel cell (BCR-MFC) was developed for simultaneous aerobic toluene vapor biodegradation, nitrification, heterotrophic denitrification, and electricity generation. The BCR-MFC was tested under various inlet loading rates (ILRs) of toluene to the bio-cathode chamber. The maximum elimination capacity (EC_max) reached 248 g m⁻³ h⁻¹ at an ILR of 456 g m⁻³ h⁻¹. Optimal performance occurred at an ILR of 306 g m⁻³ h⁻¹, yielding an EC of 208 g m⁻³ h⁻¹ and a voltage output of 522 mV based on the integrated efficiency parameter (ε). The highest power density (4.8 W m⁻³) was obtained at the lowest ILR (70 ± 6 g m⁻³ h⁻¹). At an ILR of 316 ± 30 g m⁻³ h⁻¹, NH₄⁺–N decreased from 1.3 g L⁻¹ to 0.134 g L⁻¹, and NOₓ⁻–N from 1.3 g L⁻¹ to 0.239 g L⁻¹. Microbial community analysis revealed increased abundance of toluene-degrading bacteria, heterotrophic denitrifiers, and nitrifiers, including Pseudomonas, Paracoccus, Zoogloea, and Bacillus. These results highlight the BCR-MFC ability to couple efficient toluene degradation with nitrogen compound removal while producing bioelectricity, facilitated by the coexistence of essential microbial species in the bio-cathode chamber.

开发了一种集成式泡塔反应器-微生物燃料电池（BCR-MFC），用于同时进行好氧甲苯蒸汽生物降解、硝化、异养反硝化和发电。对BCR-MFC进行了不同进口甲苯负载量（ILRs）下的生物阴极室测试。在ILR为456 g m−3 h−1时，最大消能（ECmax）达到248 g m−3 h−1。基于综合效率参数（ε），最佳性能出现在ILR为306 g m−3 h−1时，产生EC为208 g m−3 h−1，输出电压为522 mV。在最小ILR（70±6 g m−3 h−1）下获得最高的功率密度（4.8 W m−3）。当ILR为316±30 g m−3 h−1时，nh4 + -N从1.3 g L−1降至0.134 g L−1，NOₓ−-N从1.3 g L−1降至0.239 g L−1。微生物群落分析显示，甲苯降解菌、异养反硝化菌和硝化菌的丰度增加，包括假单胞菌、副球菌、动物芽孢杆菌和芽孢杆菌。这些结果强调了BCR-MFC在产生生物电的同时能够高效地降解甲苯和去除氮化合物，这得益于生物阴极室中必需微生物物种的共存。

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引用次数: 0

A POC label-free biosensor to detect point mutations in the rpoB gene of Mycobacterium tuberculosis from RR-TB raw sputum samples 一种无POC标记的生物传感器检测结核分枝杆菌rpoB基因点突变

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-10-21 DOI: 10.1016/j.bioelechem.2025.109138

Muhammad Nauman Sharif , Saman Taufiq , Manzar Sohail , Shah Rukh Abbas

Rifampicin-resistant and multidrug-resistant tuberculosis (RR/MDR-TB) remain pressing global health concerns. Conventional diagnostics such as culture and GeneXpert are limited by high cost, infrastructure requirements, and lengthy procedures, highlighting the need for rapid and reliable molecular detection platforms. We developed an electrochemical biosensor for the selective detection of wild-type and clinically relevant point mutations (codons 516 and 531) in the rpoB gene of Mycobacterium tuberculosis. Thiolated gene-specific ssDNA probes were immobilized on gold nanoparticle-modified carbon screen-printed electrodes. The working principle of the designed biosensor relies on hybridization between the immobilized ssDNA probes and their complementary target sequences, which induces a marked decrease in electrochemical signals measured using Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV), thereby confirming high mutation specificity. The biosensor achieved detection limits of 6.01 pM and 6.48 pM for codons 516 and 531, respectively, with strong linearity (R² = 0.963 and 0.957). It successfully identified MDR-TB directly from raw sputum samples, eliminating the need for sample pretreatment. This highly sensitive (∼6 pM), mutation-specific electrochemical platform offers an accurate and rapid molecular diagnostic for RR/MDR-TB. Its ability to distinguish specific mutations and operate directly on clinical samples underscores its promise for point-of-care application.

耐利福平和耐多药结核病（RR/MDR-TB）仍然是紧迫的全球卫生问题。传统的诊断方法，如培养和GeneXpert，受到高成本、基础设施要求和冗长程序的限制，突出了对快速可靠的分子检测平台的需求。我们开发了一种电化学生物传感器，用于选择性检测结核分枝杆菌rpoB基因野生型和临床相关的点突变（密码子516和531）。将硫代基因特异性ssDNA探针固定在金纳米颗粒修饰的碳丝网印刷电极上。所设计的生物传感器的工作原理依赖于固定的ssDNA探针与其互补的靶序列之间的杂交，这使得使用循环伏安法（CV）和差分脉冲伏安法（DPV）测量的电化学信号显着减少，从而证实了高突变特异性。该传感器对密码子516和531的检出限分别为6.01 pM和6.48 pM，线性关系良好（R2 = 0.963和0.957）。它成功地直接从原始痰样本中鉴定出耐多药结核病，消除了样品预处理的需要。这种高度敏感（~ 6 pM）、突变特异性的电化学平台为耐药/耐多药结核病提供了准确、快速的分子诊断。它能够区分特定的突变并直接对临床样本进行操作，这强调了它在护理点应用的前景。

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引用次数: 0

Self-charging half-cell biocapacitor utilizing short-circuit current for enhanced sensitivity and simplified operation of an enzymatic biosensor 利用短路电流的自充电半电池生物电容器，用于提高酶生物传感器的灵敏度和简化操作

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-10-21 DOI: 10.1016/j.bioelechem.2025.109146

Yihang Jiao , Shu Shimotoyodome , Hideo Shimodaira , Kenta Takano , Haruta Togashi , Keita Mitsui , Aika Saito , Kuniaki Nagamine

This study demonstrates for the first time the operational principle of a highly sensitive half-cell enzymatic self-charging biocapacitor that utilizes short-circuit current in glucose detection. The sensor consists of two electrodes: flavin adenine dinucleotide-dependent glucose dehydrogenase/mediator-modified electrode and a Ag/AgCl electrode. When these electrodes are disconnected in an aqueous solution containing glucose (as a model analyte), charge accumulates over time due to the potential difference between the electrodes. Upon reconnecting the electrodes (short-circuiting), a discharge current is generated, which depends on the glucose concentration and the charging time. Notably, the detection limit of this glucose sensor reached 0.092 μM with a 5-min charging time. This work introduces an innovative approach to electrochemical sensing with significant ultrasensitive analyte detection.

本研究首次展示了利用短路电流进行葡萄糖检测的高灵敏度半细胞酶自充电生物电容器的工作原理。该传感器由两个电极组成：黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶/介质修饰电极和Ag/AgCl电极。当这些电极在含有葡萄糖的水溶液中断开时（作为模型分析物），由于电极之间的电位差，电荷随着时间的推移而积累。在重新连接电极（短路）时，会产生放电电流，放电电流取决于葡萄糖浓度和充电时间。值得注意的是，该传感器的检测限为0.092 μM，充电时间为5 min。这项工作介绍了一种创新的电化学传感方法，具有重要的超灵敏分析物检测。

引用次数: 0

Ultrasensitive electrochemiluminescence immunosensor based on gold-functionalised NiFe layered double hydroxide and electro-negative mesoporous silica for prostate-specific antigen detection 基于金功能化NiFe层状双氢氧化物和电负介孔二氧化硅的超灵敏电化学发光免疫传感器用于前列腺特异性抗原检测。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-10-18 DOI: 10.1016/j.bioelechem.2025.109145

Dongcheng Yang , Huan Wang , Caiyu Wang , Xinyu Liu , Lihua Hu , Hongmin Ma , Dan Wu , Xiang Ren , Yuyang Li , Qin Wei

Electrochemiluminescence (ECL) technology has become an essential analytical methodology in biomolecular detection, attaining both profound research advancements and extensive practical applications in this domain. Herein, we used carboxylated mesoporous silica (MSN-COOH) as a container to encapsulate Tris(2,2-bipyridyl)ruthenium(II)²⁺ (Ru(bpy)₃²⁺)—a luminophore—in its pores to achieve enrichment (RuMSN). The reactive intermediates Ru(bpy)₃³⁺ and TPrA^•+ are attracted by the electro-negative carboxyl group on the MSN-COOH surface, substantially reducing the reaction distance and improving the ECL response efficiency. The flower-like NiFe layered double hydroxide (NiFe-LDH) nanostructure possesses significantly high specific surface area and good electrocatalytic performance, while gold nanoparticles also demonstrate excellent electrical conductivity and biocompatibility. To enhance sensor sensitivity, gold nanoparticles were loaded onto the NiFe-LDH surface forming the Au@NiFe-LDH composite, which improves performance by enhancing conductive properties and increasing antigen-antibody binding sites. This study established a sandwich-configuration electrochemiluminescence detection platform capable of highly sensitive PSA analysis.The biosensor demonstrated excellent stability, specificity and selectivity, with a linear detection range of 0.1 pg/mL-50 ng/mL and a detection limit as low as 63 fg/mL (S/N = 3). This confirms the clinical application value of the detection system in the early screening of prostate cancer.

电化学发光（ECL）技术已成为生物分子检测中必不可少的分析方法，在该领域取得了深刻的研究进展和广泛的实际应用。本文采用羧化介孔二氧化硅（MSN-COOH）作为容器，将Tris（2,2-联吡啶基）钌（II）2+ (Ru(bpy)32+)-发光团包封在其孔隙中，实现富集（RuMSN）。反应中间体Ru(bpy)33+和TPrA•+被MSN-COOH表面的电负羧基吸引，大大缩短了反应距离，提高了ECL响应效率。这种花状NiFe层状双氢氧化物（NiFe- ldh）纳米结构具有显著的高比表面积和良好的电催化性能，而金纳米颗粒也具有优异的导电性和生物相容性。为了提高传感器的灵敏度，将金纳米颗粒加载到nfe - ldh表面形成Au@NiFe-LDH复合材料，通过增强导电性能和增加抗原-抗体结合位点来提高性能。本研究建立了一个具有高灵敏度PSA分析能力的三明治结构电化学发光检测平台。该传感器具有良好的稳定性、特异性和选择性，线性检测范围为0.1 pg/mL ~ 50 ng/mL，检出限低至63 fg/mL （S/N = 3）。这证实了该检测系统在前列腺癌早期筛查中的临床应用价值。

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引用次数: 0

Biofunctionalized reduced (graphene quantum dots-graphene oxide) nanohybrid modified screen printed electrode for electrochemical detection of thyroid stimulating hormone 生物功能化还原（石墨烯量子点-氧化石墨烯）纳米杂化修饰的丝网印刷电极用于促甲状腺激素的电化学检测

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-10-17 DOI: 10.1016/j.bioelechem.2025.109141

Sudesh Yadav , Vikash Sharma , Rohit Kumar , Gajjala Sumana , Rajesh

A selective and sensitive surface modified screen printed carbon electrode (SPCE) with reduced (graphene quantum dots-graphene oxide), r(GQD/GO) nanohybrid, is reported for the electrochemical detection of thyroid stimulating hormone (TSH). TSH specific monoclonal antibody was immobilized on r(GQD/GO)/SPCE by wet method to obtain a bioelectrode. The bioelectrode was characterized with microscopic, spectroscopic and electrochemical techniques and the differential pulse voltammetry (DPV) measurement was used for the detection of TSH in phosphate buffer saline 0.1 M PBS (pH 7.4; 0.1 M KCl). The bioelectrode showed a linear DPV response at 0.16 V to TSH over the concentration range of 1 nIU mL⁻¹-100 μIU mL⁻¹ with a lowest detection limit of 0.18 nIU mL⁻¹ and sensitivity of 2.15 μA per decade of TSH concentration. The biosensing response with potential interferents indicates that the sensing platform is highly specific for the detection of TSH. The wide range of DPV response with good sensitivity and specificity make this bioelectrode potentially useful for abnormal upper and lower TSH level detection pertaining to both hypo and hyperthyroidism.

报道了一种具有还原（石墨烯量子点-氧化石墨烯），r（GQD/GO）纳米杂化物的表面修饰丝网印刷碳电极（SPCE），用于促甲状腺激素（TSH）的电化学检测。将TSH特异性单克隆抗体湿法固定在r(GQD/GO)/SPCE上，获得生物电极。采用显微、光谱和电化学技术对生物电极进行了表征，并采用差分脉冲伏安法（DPV）测定了0.1 M PBS （pH 7.4; 0.1 M KCl）中磷酸缓冲盐水中TSH的含量。该生物电极在0.16 V电压下，在1 nIU mL−1 ~ 100 μIU mL−1浓度范围内对TSH具有线性DPV响应，最低检测限为0.18 nIU mL−1，灵敏度为2.15 μA / 10年TSH浓度。具有潜在干扰的生物传感响应表明，该传感平台对TSH检测具有高度特异性。DPV反应范围广，具有良好的敏感性和特异性，使该生物电极可用于检测甲状腺功能低下和甲状腺功能亢进的异常TSH水平。

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引用次数: 0

Resolving nanosecond kinetics of the optical membrane potential in pulsed electric fields 解析脉冲电场中光膜电位的纳秒动力学

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-10-17 DOI: 10.1016/j.bioelechem.2025.109143

Iurii Semenov , Giedre Silkuniene , Mantas Silkunas , Olga N. Pakhomova , Joel N. Bixler , Bennett L. Ibey , Mark A. Keppler , Andrei G. Pakhomov

The primary bioeffect of pulsed electric fields (PEFs) is cell membrane charging, which determines a plethora of downstream events. While the kinetics of de- and hyperpolarization at the cathode- and anode-facing cell hemispheres should be the same, studies using pulsed laser strobe microscopy have reported asymmetrical charging in mammalian cells exposed to brief PEFs. To validate these anomalies, we measured charging kinetics with FluoVolt and Di-8-ANEPPS potentiometric dyes in spheroidal CHO cells exposed to 1-μs PEFs at 0–350 V/cm. Both dyes exhibited emission changes dependent on the electric field incidence angle, symmetrically at cathodic and anodic hemispheres. The changes scaled linearly with electric field strength up to at least 350 V/cm for Di-8-ANEPPS, but only to ∼200 V/cm for FluoVolt. Time constants measured from the optical membrane potential kinetics did not differ between the dyes, between cathodic and anodic cell poles, or between membrane charging and relaxation. The time constants followed the theoretical dependence on cell radius for 1.6 μF/cm² specific membrane capacitance and 0.4 S/m intracellular conductance. No charging anomalies were observed. Both dyes proved suitable for characterizing nanosecond charging kinetics, with FluoVolt being more sensitive for low-noise readings, while Di-8-ANEPPS allowed for dynamic measurements within a broader range.

脉冲电场（PEFs）的主要生物效应是细胞膜充电，它决定了大量的下游事件。虽然阴极和阳极细胞半球的去极化和超极化动力学应该是相同的，但使用脉冲激光频闪显微镜的研究已经报道了哺乳动物细胞暴露于短暂的pef下的不对称充电。为了验证这些异常，我们用FluoVolt和Di-8-ANEPPS电位染料测量了球形CHO细胞在0-350 V/cm下暴露于1 μs PEFs的充电动力学。两种染料的发射随电场入射角的变化而变化，在阴极和阳极半球对称。对于Di-8-ANEPPS，变化随电场强度呈线性增长，至少达到350 V/cm，但对于FluoVolt，变化仅为~ 200 V/cm。从光学膜电位动力学测量的时间常数在染料之间、阴极和阳极电池极点之间、膜充电和弛豫之间没有差异。在1.6 μF/cm2比膜电容和0.4 S/m胞内电导上，时间常数遵循细胞半径的理论依赖关系。未观察到电荷异常。事实证明，这两种染料都适合表征纳秒级充电动力学，其中FluoVolt对低噪声读数更敏感，而Di-8-ANEPPS允许在更宽的范围内进行动态测量。

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引用次数: 0

Engineering conducting polymer-based interfaces for high-performance microbial electrochemical systems 工程导电聚合物为基础的高性能微生物电化学系统的界面。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-10-17 DOI: 10.1016/j.bioelechem.2025.109142

Abdullah , Divine Yufetar Shyntum , Sara Shakibania , Taral Patel , Katarzyna Krukiewicz

Poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) is a widely recognized conducting polymer, especially in the field of electromicrobiology. Nonetheless, its inherently moderately hydrophobic and smooth surface poses a challenge for bacterial adhesion and biofilm formation, thereby restricting the growth and charge transfer of electroactive bacteria. To overcome these limitations, PEDOT:PSS films were modified with selected salts (FeCl₃, MgSO₄, MnCl₂, CaCl₂, BaCl₂) to improve bacterial attachment, biofilm formation and electrochemical performance, including charge storage capacity and charge transfer efficiency. Among the formed coatings, PEDOT:PSS@Ca demonstrated the highest charge storage capacity of 5.1 ± 1.0 mC/cm² in the presence of S. oneidensis MR-1, representing a three orders of magnitude increase when compared with pristine PEDOT:PSS. The improvement in biofilm formation was significant, with PEDOT:PSS@Ca attaining 55.0 ± 1.3 % biofilm formation and 92.8 ± 3.1 % bacterial viability, representing a considerable increase compared to unmodified PEDOT:PSS (4.1 ± 1.2 % biofilm formation and 63.1 ± 7.2 % viability). Calcium modification notably enhanced electron transfer by decreasing the charge transfer resistance, thereby promoting more effective electron exchange at the electrode interface. The findings indicate that functionalization of PEDOT:PSS with metal salts, and particularly CaCl₂, is an effective approach for enhancing microbial fuel cell performance.

聚（3,4-乙烯二氧噻吩）：聚（苯乙烯磺酸酯）（PEDOT:PSS）是一种广泛认可的导电聚合物，特别是在电微生物学领域。然而，其固有的适度疏水性和光滑表面对细菌的粘附和生物膜的形成提出了挑战，从而限制了电活性细菌的生长和电荷转移。为了克服这些限制，PEDOT:PSS薄膜用选定的盐（FeCl3, MgSO4, MnCl2, CaCl2, BaCl2）修饰，以改善细菌附着，生物膜形成和电化学性能，包括电荷存储容量和电荷转移效率。在形成的涂层中，PEDOT:PSS@Ca在S. oneidensis MR-1存在下的电荷存储容量最高，为5.1±1.0 mC/cm2，与原始PEDOT:PSS相比提高了3个数量级。生物膜形成的改善是显著的，PEDOT:PSS@Ca达到55.0±1.3%的生物膜形成和92.8±3.1%的细菌活力，与未经修饰的PEDOT:PSS（4.1±1.2%的生物膜形成和63.1±7.2%的活力）相比，有了显著的提高。钙改性通过降低电荷转移阻力显著增强电子转移，从而促进电极界面更有效的电子交换。研究结果表明，金属盐特别是CaCl2功能化PEDOT:PSS是提高微生物燃料电池性能的有效途径。

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