Bioelectrochemistry最新文献

{"title":"Cytochrome P450 electrochemical biosensors transforming in vitro metabolism testing – Opportunities and challenges","authors":"Carina S.P. Vieira,&nbsp;Marcela A. Segundo,&nbsp;Alberto N. Araújo","doi":"10.1016/j.bioelechem.2025.108913","DOIUrl":"10.1016/j.bioelechem.2025.108913","url":null,"abstract":"<div><div>The ability of the living world to flourish in the face of constant exposure to dangerous chemicals depends on the management ability of a widespread group of enzymes known as heme-thiolate monooxygenases or cytochrome P450 superfamily. About three-quarters of all reactions determining the metabolism of endogenous compounds, of those carried in foods, of taken drugs, or even of synthetic chemicals discarded into the environment depend on their catalytic performance. The chromatographic and (photo)luminometric methods routinely used as predictive and analytical tools in laboratories have significant drawbacks ranging from limited shelf-life of reagents, use of synthetic substrates, laborious and tedious procedures for highly sensitive detection. In this review, alternative electrochemical biosensors using the cytochrome P450 enzymes as bio-element are emphasized in their main aspects as well regarding their implementation and usefulness. Despite the various schemes proposed for the implementation, reports on real applications are scant for several reasons, including low reaction rates, broad substrate specificity, uncoupling reactions occurrence, and the need for expensive electron transfer partners to promote electron transfer. Finally, the prospect for future developments is introduced, focusing on integrating miniaturized systems with electrochemical techniques, alongside optimizing enzyme immobilization methods and electrode modifications to improve enzymatic stability and enhance sensor reliability. This progress represents a crucial step towards the creation of portable biosensors that mimic human physiological responses, supporting the precision medicine approach.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108913"},"PeriodicalIF":4.8,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-time prediction of heparin concentration in blood extracorporeal circulation by relaxation time distribution (RTD)","authors":"Soichiro Ueno ,&nbsp;Daisuke Kawashima ,&nbsp;Katsuhiro Matsuura ,&nbsp;Hiromichi Obara ,&nbsp;Ryou Tanaka ,&nbsp;Masahiro Takei","doi":"10.1016/j.bioelechem.2025.108912","DOIUrl":"10.1016/j.bioelechem.2025.108912","url":null,"abstract":"&lt;div&gt;&lt;div&gt;Heparin concentration &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;c&lt;/mi&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; in a blood extracorporeal circulation has been real-timely predicted based on the relaxation strength &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mrow&gt;&lt;mi&gt;Δ&lt;/mi&gt;&lt;mi&gt;ε&lt;/mi&gt;&lt;/mrow&gt;&lt;mi&gt;m&lt;/mi&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; at relaxation frequency &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;f&lt;/mi&gt;&lt;mi&gt;m&lt;/mi&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; extracted by relaxation time distribution (RTD). The simulated extracorporeal circulation was conducted to optimize the number of &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mrow&gt;&lt;mi&gt;Δ&lt;/mi&gt;&lt;mi&gt;ε&lt;/mi&gt;&lt;/mrow&gt;&lt;mi&gt;m&lt;/mi&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; for the prediction of &lt;em&gt;c&lt;/em&gt; using the porcine whole blood (WB) and low-leukocyte and −platelet blood (LLPB) under the condition of the gradual increment of &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;c&lt;/mi&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; from 0 to 8 U/mL with constant flow rate and blood temperature. The experimental results show that among the three relaxation strengths &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mrow&gt;&lt;mi&gt;Δ&lt;/mi&gt;&lt;mi&gt;ε&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;1&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;, &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mrow&gt;&lt;mi&gt;Δ&lt;/mi&gt;&lt;mi&gt;ε&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; and &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mrow&gt;&lt;mi&gt;Δ&lt;/mi&gt;&lt;mi&gt;ε&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; (in ascending order of frequency), &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mrow&gt;&lt;mi&gt;Δ&lt;/mi&gt;&lt;mi&gt;ε&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; at &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;f&lt;/mi&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; = 5.2 ∼ 6.2 MHz and &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mrow&gt;&lt;mi&gt;Δ&lt;/mi&gt;&lt;mi&gt;ε&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; at &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;f&lt;/mi&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; = 42 ∼ 50 MHz were correlated to &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;c&lt;/mi&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;. The &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mrow&gt;&lt;mi&gt;Δ&lt;/mi&gt;&lt;mi&gt;ε&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; was decreasing with increasing &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;c&lt;/mi&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; in both cases, which was influenced by the plasma macromolecular concentrations, while the &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mrow&gt;&lt;mi&gt;Δ&lt;/mi&gt;&lt;mi&gt;ε&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; was increased with increasing &lt;em&gt;c&lt;/em&gt; in WB case but was hardly changed in LLPB case because the &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mrow&gt;&lt;mi&gt;Δ&lt;/mi&gt;&lt;mi&gt;ε&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; is influenced by the blood cell concentrations and the shape changes of blood cell membranes. Heparin concentration &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;c&lt;/mi&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; is estimated by the linear regression formula &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msup&gt;&lt;mrow&gt;&lt;mi&gt;c&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow&gt;&lt;mi&gt;P&lt;/mi&gt;&lt;mi&gt;R&lt;/mi&gt;&lt;mi&gt;E&lt;/mi&gt;&lt;/mrow&gt;&lt;/msup&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;msub&gt;&lt;mi&gt;a&lt;/mi&gt;&lt;mn&gt;1&lt;/mn&gt;&lt;/msub&gt;&lt;msub&gt;&lt;mrow&gt;&lt;mo&gt;(&lt;/mo&gt;&lt;mi&gt;Δ&lt;/mi&gt;&lt;mi&gt;ε&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;mo&gt;-&lt;/mo&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;Δ&lt;/mi&gt;&lt;mi&gt;ε&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/mrow&gt;&lt;mrow&gt;&lt;mi&gt;c&lt;/mi&gt;&lt;mo&gt;=&lt;/mo&gt;&lt;mn&gt;0&lt;/mn&gt;&lt;/mrow&gt;&lt;/msubsup&gt;&lt;mrow&gt;&lt;mo&gt;)&lt;/mo&gt;&lt;mo&gt;+&lt;/mo&gt;&lt;/mrow&gt;&lt;msub&gt;&lt;mi&gt;a&lt;/mi&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;mrow&gt;&lt;mo&gt;(&lt;/mo&gt;&lt;msub&gt;&lt;mrow&gt;&lt;mi&gt;Δ&lt;/mi&gt;&lt;mi&gt;ε&lt;/mi&gt;&lt;/mrow&gt;&lt;mn&gt;3&lt;/mn&gt;&lt;/msub&gt;&lt;mo&gt;-&lt;/mo&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108912"},"PeriodicalIF":4.8,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of micro-nanostructured film with antibacterial, anticorrosive and thermal conductivity properties on copper surface","authors":"Li Lai,&nbsp;Muqiu Xia,&nbsp;Mengyu Fu,&nbsp;Yuanyuan Gao,&nbsp;Jiahao Sun,&nbsp;Guangzhou Liu,&nbsp;Shiqiang Chen","doi":"10.1016/j.bioelechem.2025.108905","DOIUrl":"10.1016/j.bioelechem.2025.108905","url":null,"abstract":"<div><div>A micro-nano sharkskin like film (Cu-MNS-FA) was synthesized on copper surface through chemical etching followed by formate passivation, and its anticorrosive, antibacterial and thermal conductivity properties were investigated. Results show that after 7 d of exposure to nature, <em>Pseudomonas aeruginosa</em> and <em>Desulfovibrio vulgaris</em> seawater, the charge transfer resistance of Cu-MNS-FA is more than three times higher than that of unmodified copper. In particular, in <em>D. vulgaris</em> seawater, the <em>R</em>_ct value of modified copper is 7 times higher than that of unmodified copper after the same exposure duration. The counts of sessile cells, specifically <em>P. aeruginosa</em> and <em>D. vulgaris</em>, on the surface of modified copper are reduced by &gt; 88 % after 3 days of immersion. Furthermore, thermal conductivity remains 10 % higher than that of untreated copper after 7 d of immersion. This film improves the performance characteristics of copper in seawater heat exchange systems.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108905"},"PeriodicalIF":4.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Print-Light-Synthesis of ruthenium oxide thin film electrodes for electrochemical sensing applications","authors":"Stefano Gianvittorio ,&nbsp;Marco Malferrari ,&nbsp;Horst Pick ,&nbsp;Stefania Rapino ,&nbsp;Andreas Lesch","doi":"10.1016/j.bioelechem.2025.108909","DOIUrl":"10.1016/j.bioelechem.2025.108909","url":null,"abstract":"<div><div>Print-Light-Synthesis (PLS) combines the inkjet printing of a ruthenium precursor ink with the simultaneous photo-induced generation of ruthenium oxide films. During PLS, inkjet-printing generates on conductive as well as insulating substrates micrometer-thin reaction volumes that contain with high precision defined precursor loadings. Upon direct UV light irradiation, the Ru precursor converts to RuO₂ while all other ink components escape in the gas phase. No post PLS processes are required, and the as-obtained RuO₂ films can be immediately used as electrochemical devices. Two-dimensional RuO₂ patterns with micrometric resolution and highly-controlled ruthenium loadings (few µg/cm²) are realized. Thin RuO₂ films are generated on insulating substrates, such as polyimide, as well as individual RuO₂ particles on conductive substrates, such as graphene layers. The RuO₂ films are characterized by electron microscopy and spectroscopic techniques. The sensoristic applicability of the PLS-RuO₂ electrodes is demonstrated by potentiometric pH sensing in cell cultures and amperometric detection of L-cysteine. For pH sensing the RuO₂ film electrodes show Nernstian sensitivity. L-cysteine detection of RuO₂-modified graphene electrodes showed an electrocatalytical effect and resulted in the possibility of selectively detecting L-Cysteine also in presence of the interfering compound uric acid.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108909"},"PeriodicalIF":4.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced detection of Cystatin C for predicting adverse outcomes in gestational diabetes mellitus using a point-of-care immunosensor","authors":"Ya Jin ,&nbsp;Yongmei Chen ,&nbsp;Xiaoqin Li ,&nbsp;Songjun Han ,&nbsp;Lei Mou ,&nbsp;Nan Li","doi":"10.1016/j.bioelechem.2025.108907","DOIUrl":"10.1016/j.bioelechem.2025.108907","url":null,"abstract":"<div><div>Cystatin C (Cys-C) is emerging as a critical biomarker for assess gestational diabetes mellitus (GDM), a condition that significantly impacts maternal and fetal health. In this study, we developed a novel label-free electrochemical immunosensor designed for point-of-care applications, offering lower reagent consumption and rapid detection of Cys-C in pregnant women with GDM. Compared to traditional enzyme-linked immunosorbent assays (ELISA), the sensor demonstrates enhanced sensitivity, reduced reagent usage, and faster detection. In a cohort study involving 150 pregnant women with GDM and 150 healthy controls, serum Cys-C levels were analyzed using the developed sensor. Serum samples were collected and analyzed for Cys-C levels using our immunosensor. Serum Cys-C levels were significantly elevated in GDM patients compared to controls (P &lt; 0.05), and higher levels were observed in GDM complicated by pregnancy-induced hypertension (PIH) and fetal growth restriction (FGR). Furthermore, elevated Cys-C levels were positively correlated with adverse pregnancy outcomes, including premature birth, fetal distress, and cesarean section (P &lt; 0.05). This study underscores the potential of Cys-C as a reliable biomarker for GDM and highlights the advantages of our novel sensor for quick, accurate, and scalable GDM diagnosis and management.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108907"},"PeriodicalIF":4.8,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conductive and flexible gold-coated polylactic acid nanofiber-based electrochemical aptasensor for monitoring cortisol level in sweat and saliva","authors":"Mkliwa Koumbia,&nbsp;Takai Madoka","doi":"10.1016/j.bioelechem.2025.108908","DOIUrl":"10.1016/j.bioelechem.2025.108908","url":null,"abstract":"<div><div>Conductive nanofibers can exhibit excellent mechanical properties such as flexibility, elasticity, porosity, large surface area-to-volume ratio, etc making them suitable for a wide range of applications including biosensor development. Their large surface area provides more active sites for immobilization of large amount of bioreceptors enabling more interaction sites with the target analytes, enhancing sensitivity and detection capabilities. However, engineering conductive nanofibers with such excellent properties is challenging limiting their effective deployment for intended applications. In this research, we propose a novel approach for easy fabrication of highly conductive and flexible nanofiber leveraging the electrospinning, electroless deposition and have applied it to cortisol monitoring; a common biomarker for stress which is often quantified through enzyme-linked immunoassays using blood or saliva samples. By adopting the nanofiber sheet as a transducer for aptamer immobilization and cortisol sensing our developed biosensor was able to detect cortisol in buffer, artificial saliva, and artificial sweat within five minutes, from 10 pg/mL to 10 µg/mL (27.59 pM to 27.59 µM) with a low detection limit of 1 pg/ml (2.76 pM). The Au-coated PLA nanofiber-based electrochemical biosensor’s flexibility allows for compact manufacturing, rendering it an optimal choice for integration into point-of-care testing and wearable systems.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108908"},"PeriodicalIF":4.8,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized detection of calcium ion in serum using constant potential coulometry with metastable liquid–liquid contact doping enhanced PEDOT: PSS ink","authors":"Suyun Wei ,&nbsp;Jie Zhao ,&nbsp;Shaojun Ke ,&nbsp;Mingjia Zou ,&nbsp;Qingze Han ,&nbsp;Guofeng Cui","doi":"10.1016/j.bioelechem.2025.108903","DOIUrl":"10.1016/j.bioelechem.2025.108903","url":null,"abstract":"<div><div>Highly stable calcium ion selective electrodes (Ca²⁺-ISEs) were developed by drop-casting a layer of poly(3,4-ethylenedioxythiophene): polystyrene sulfonate (PEDOT: PSS) as an ion-to-electron transfer layer onto Au electrode. The conductive PEDOT: PSS ink was prepared using a metastable liquid–liquid contact (MLLC) doping method, which induced phase separation, removed excess PSS, and significantly enhanced charge transfer kinetics and conductivity. The resulting Ca²⁺-ISEs exhibited excellent electrochemical performance. Potentiometric studies revealed a significant sensitivity of 33.1 ± 0.98 mV/decade (N = 3) with a high potential stability of 3.16 ± 2.53 μV/h. Importantly, Ca²⁺-ISEs combined with the constant potential coulometry method, the lower detection limit was optimized to 8.527 × 10⁻⁸ M (LOD = 3σ/s, N = 3). The performance of the Ca²⁺-ISE system was evaluated in inactivated fetal bovine serum using constant potential coulometry, demonstrating the highest measurement accuracy compared to potentiometric and chronoamperometric. The enhanced PEDOT: PSS-MLLC based Ca²⁺-ISEs combined with the constant potential coulometry method developed in this research demonstrate considerable potential for clinical applications in blood ion analysis.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108903"},"PeriodicalIF":4.8,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile preparation of bifunctional monolayers through diazonium grafting and “click” postfunctionalization: A first step towards efficient aptasensing interfaces","authors":"Andra Mihaela Onaş ,&nbsp;Andreea Mădălina Pandele ,&nbsp;Anamaria Hanganu ,&nbsp;Ciprian Victor Florea ,&nbsp;Horia Iovu ,&nbsp;Matei D. Raicopol ,&nbsp;Luisa Pilan","doi":"10.1016/j.bioelechem.2025.108904","DOIUrl":"10.1016/j.bioelechem.2025.108904","url":null,"abstract":"<div><div>Herein, we present an efficient approach for developing electrochemical aptasensing interfaces, by “click” postfunctionalization of phenylethynyl-grafted glassy carbon substrates with mixed monolayers containing biorecognition elements and phosphorylcholine zwitterionic groups. Typically, controlling the composition of multicomponent surface layers by grafting from a mixture of aryldiazonium salts is challenging due to differences in their chemical reactivity. Our approach circumvents this issue by employing the electrochemical reduction of a single aryldiazonium salt containing a silyl-protected alkyne group followed by deprotection, to create phenylethynyl monolayers which can subsequently accommodate the concurrent immobilization of bioreceptors and zwitterionic groups through “click” postfunctionalization. We show that the surface ratio of the components in the bifunctional monolayers, estimated through XPS and electrochemical methods, can be accurately controlled by adjusting the mole ratio of the corresponding azide reagents in the “click” coupling solution. Moreover, electrochemical impedance spectroscopy and fluorescence microscopy investigations on bifunctional monolayers containing ssDNA and phosphorylcholine groups reveal that they effectively prevent nonspecific protein adsorption, while maintaining sufficiently low impedance to facilitate electrochemical detection. Finally, we demonstrate that proof of concept aptasensing interfaces based on binary layers containing a ferrocene-tagged cocaine/quinine aptamer and phosphorylcholine groups exhibit a trade-off between an improved analytical response and antifouling efficiency.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108904"},"PeriodicalIF":4.8,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and receptor-free Prussian blue electrochemical sensor for the detection of pathogenic bacteria in blood","authors":"Sriramprabha Ramasamy,&nbsp;Sekar Madhu,&nbsp;Jungil Choi","doi":"10.1016/j.bioelechem.2025.108902","DOIUrl":"10.1016/j.bioelechem.2025.108902","url":null,"abstract":"<div><div>Bloodstream bacterial infections, a major health concern due to rising sepsis rates, require prompt, cost-effective diagnostics. Conventional methods, like CO₂-based transduction, face challenges such as volatile metabolites, delayed gas-phase signaling, and the need for additional instruments, whereas electrochemical sensors provide rapid, sensitive, and efficient real-time detection. In this study, we developed a bioreceptor-free Prussian blue (PB) sensor platform for real-time bacterial growth monitoring in blood culture. PB thin films were electrodeposited onto a screen-printed carbon electrode (SPCE) via cyclic voltammetry (CV) technique under optimal conditions. The electrochemical performance of PB/SPCE was assessed using differential pulse voltammetry (DPV) against exoelectrogenic bacteria, including <em>E. coli</em>, <em>P. aeruginosa</em>, <em>S. aureus,</em> and <em>E. faecalis</em>. The proposed sensor exhibited surface-controlled electrochemical kinetics and bacteria-driven metal reduction from PB to Prussian white (PW), facilitated by extracellular electron transfer (EET). It showed significant sensitivity with an extensive detection range of 10²–10⁸ CFU/mL for <em>E. coli</em> and <em>S. aureus</em>, and 10³–10⁸ CFU/mL for <em>P. aeruginosa</em> and <em>E. faecalis</em>, with reliable detection limits. The sensor accessed the viability of the pathogen within 3 hrs, offering a rapid, efficient alternative to traditional, labor-intensive methods for blood-based diagnostics.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108902"},"PeriodicalIF":4.8,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A carbon fiber modified with tin oxide/graphitic carbon nitride as an electrochemical indirect competitive immuno-sensor for ultrasensitive aflatoxin M1 detection","authors":"Iram Naz ,&nbsp;Muhammad Nasir ,&nbsp;Mian Hasnain Nawaz ,&nbsp;Silvana Andreescu ,&nbsp;Akhtar Hayat ,&nbsp;Farhat Jubeen","doi":"10.1016/j.bioelechem.2025.108898","DOIUrl":"10.1016/j.bioelechem.2025.108898","url":null,"abstract":"<div><div>The importance of developing multifunctional nanomaterials for sensing technologies is increasing with the arrival of nanotechnology. In this study, we describe the introduction of novel nanoprobe electro-active material into the architecture of an electrochemical immuno-sensor. Based on the electrochemical immuno-sensor, functionalized tin oxide/graphitic carbon nitride nanocomposite (fSnO₂/g-C₃N₄) was synthesized and then analyte specific anti-aflatoxin M₁ monoclonal antibody (AFM₁-ab) combined to form an electro-active nanoprobe (fSnO₂/g-C₃N₄/AFM₁-ab). First, aflatoxin M₁ (AFM₁) conjugated bovine serum albumin (BSA-AFM₁) was electro-oxidized on the surface of carbon fiber (CF) followed by the consequent addition of nanoprobe. The formation of nanocomposite was substantiated through various characterization techniques, Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy, X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Dynamic light scattering (DLS). Immuno-sensor fabrication was characterized via Field emission scanning electron microscopy (FE-SEM), optical microscope images, cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). This immuno-sensor demonstrated good reproducibility, selectivity, specificity and sensitivity for AFM₁ (LOD of 0.03 ng mL⁻¹). Following spiking, this immuno-sensor produced good recovery values in the range of 94–96 % against real sample, such as milk. The development of sophisticated sensing methods for a range of analytes can greatly benefit from the widespread application of this innovative immuno-sensing approach.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108898"},"PeriodicalIF":4.8,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}