Pub Date : 2026-03-01Epub Date: 2025-11-11DOI: 10.1016/j.bioelechem.2025.109165
A.D. Ettiyan , Tamilarasan K , Siva P.R. Muppala
This study presents an innovative and environmentally sustainable approach for treating pharmaceutical wastewater (PWW) using a dual-chamber microbial fuel cell (DMFC) that simultaneously generates bioelectricity. The DMFC system incorporates manganese cobalt oxide-coated carbon veil (MnCo2O4-CV) electrodes to optimize organic pollutant removal and enhance power production from PWW. The novel MnCo2O4-CV electrode coating represents a significant advancement, offering superior chemical stability, electrical conductivity, durability, large surface area, and enhanced absorption capacity. Following a systematic acclimatization, various organic loadings were investigated to identify optimal operating conditions. Results demonstrated peak performance at an organic loading of 2.0 g COD/L. Under these conditions, the system exhibited remarkable removal efficiencies for total chemical oxygen demand (TCOD), soluble chemical oxygen demand (SCOD), and total suspended solids (TSS), while generating electrical output. Performance evaluation encompassed maximum voltage, current density, power density, coulombic efficiency, and pollutant removal metrics. Microbial community analysis via 16S rRNA gene sequencing revealed a diverse bacterial community in the anodic biofilm that contributed to improved system performance.
本研究提出了一种创新和环境可持续的方法，使用双室微生物燃料电池（DMFC）同时产生生物电来处理制药废水（PWW）。DMFC系统采用锰钴氧化物涂层碳膜（MnCo2O4-CV）电极，以优化有机污染物的去除并提高PWW的发电量。新型MnCo2O4-CV电极涂层代表了一项重大进步，具有卓越的化学稳定性、导电性、耐久性、大表面积和增强的吸收能力。在系统驯化后，研究了各种有机负荷，以确定最佳操作条件。结果表明，有机负荷为2.0 g COD/L时性能最佳。在这些条件下，该系统在产生电力输出的同时，对总化学需氧量（TCOD）、可溶性化学需氧量（SCOD）和总悬浮物（TSS）表现出了显著的去除效率。性能评估包括最大电压、电流密度、功率密度、库仑效率和污染物去除指标。通过16S rRNA基因测序对微生物群落进行分析，发现阳极生物膜中的细菌群落多样性有助于提高系统性能。
{"title":"Bioelectricity generation via biodegradation of pharmaceutical wastewater using MnCo2O4-CV coated electrodes in dual-chamber microbial fuel cells","authors":"A.D. Ettiyan , Tamilarasan K , Siva P.R. Muppala","doi":"10.1016/j.bioelechem.2025.109165","DOIUrl":"10.1016/j.bioelechem.2025.109165","url":null,"abstract":"<div><div>This study presents an innovative and environmentally sustainable approach for treating pharmaceutical wastewater (PWW) using a dual-chamber microbial fuel cell (DMFC) that simultaneously generates bioelectricity. The DMFC system incorporates manganese cobalt oxide-coated carbon veil (MnCo<sub>2</sub>O<sub>4</sub>-CV) electrodes to optimize organic pollutant removal and enhance power production from PWW. The novel MnCo<sub>2</sub>O<sub>4</sub>-CV electrode coating represents a significant advancement, offering superior chemical stability, electrical conductivity, durability, large surface area, and enhanced absorption capacity. Following a systematic acclimatization, various organic loadings were investigated to identify optimal operating conditions. Results demonstrated peak performance at an organic loading of 2.0 g COD/L. Under these conditions, the system exhibited remarkable removal efficiencies for total chemical oxygen demand (TCOD), soluble chemical oxygen demand (SCOD), and total suspended solids (TSS), while generating electrical output. Performance evaluation encompassed maximum voltage, current density, power density, coulombic efficiency, and pollutant removal metrics. Microbial community analysis via 16S rRNA gene sequencing revealed a diverse bacterial community in the anodic biofilm that contributed to improved system performance.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109165"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145555869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2025-11-16DOI: 10.1016/j.bioelechem.2025.109173
Cheng Huang , Haowen Liu , Bao Jiang , Guoli Li , Xinlu Qin , Yinan Hua , Yongming Deng , Yicheng Wang , Lin Zhou
Acute kidney injury (AKI), a critical clinical syndrome marked by high incidence and mortality, is currently diagnosed mainly by serum creatinine (SCr) and blood urea nitrogen (BUN), which have high miss rates. This study innovatively proposes using urinary hydrogen peroxide (H2O2) concentration changes, caused by renal oxidative stress in AKI, as a new indicator for AKI risk assessment and treatment monitoring. Results from in vitro and AKI animal models show this indicator can quickly monitor AKI onset and drug effects in mice via electrochemical sensing technology based on Bi2S3@Cu0.1, offering a novel approach for AKI diagnosis and rehabilitation monitoring.
{"title":"Electrochemical sensor for urinary H2O2 detection to aid AKI diagnosis and treatment evaluation","authors":"Cheng Huang , Haowen Liu , Bao Jiang , Guoli Li , Xinlu Qin , Yinan Hua , Yongming Deng , Yicheng Wang , Lin Zhou","doi":"10.1016/j.bioelechem.2025.109173","DOIUrl":"10.1016/j.bioelechem.2025.109173","url":null,"abstract":"<div><div>Acute kidney injury (AKI), a critical clinical syndrome marked by high incidence and mortality, is currently diagnosed mainly by serum creatinine (SCr) and blood urea nitrogen (BUN), which have high miss rates. This study innovatively proposes using urinary hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) concentration changes, caused by renal oxidative stress in AKI, as a new indicator for AKI risk assessment and treatment monitoring. Results from in vitro and AKI animal models show this indicator can quickly monitor AKI onset and drug effects in mice via electrochemical sensing technology based on Bi<sub>2</sub>S<sub>3</sub>@Cu<sub>0.1</sub>, offering a novel approach for AKI diagnosis and rehabilitation monitoring.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109173"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145555975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2025-11-14DOI: 10.1016/j.bioelechem.2025.109169
HaoXian He , JianBing Li , JianMing Liu, LongSheng Pei, LongFei Miao, YongHai Song, LiMin Liu, Li Wang
The immobilization of enzymes is crucial for enhancing their catalytic activity and stability. Covalent organic frameworks (COF), with abundant active sites and tunable pore structures, enable effective immobilization of enzymes. Here, we designed carboxyl-functionalized COF (COF-COOH) to immobilize Cytochrome C (Cyt C), aiming to regulate the perfect pairing of the COF pore (3.67 nm) and the Cyt C dimension (2.6 nm × 3.2 nm × 3.3 nm). Meanwhile, the large amount of -COOH can increase the electrostatic and hydrogen bonding forces between COF-COOH and Cyt C. Thus, the Cyt C was efficiently loaded into COF-COOH through the post-modification method (loading efficiency = 62.37 %). The catalytic activity (kcat/Km) of Cyt C@COF-COOH toward H2O2 was significantly enhanced to 309.96 s−1 M−1 as compared to free Cyt C of 105.55 s−1 M−1. The catalytic activity of Cyt C@COF-COOH toward H2O2 still exceeds 80 % in some harsh environments (acetonitrile, dimethyl sulfoxide, tetrahydrofuran and 60 °C). The detection range of electrochemical H2O2 biosensor based on Cyt C@COF-COOH is as wide as 2.0–80 μM, and the sensitivity is as high as 0.373 μA μM−1 cm−2.
{"title":"Carboxyl-functionalized covalent organic framework with precisely matched pore size achieving effective loading of cytochrome C for electrochemical biosensors","authors":"HaoXian He , JianBing Li , JianMing Liu, LongSheng Pei, LongFei Miao, YongHai Song, LiMin Liu, Li Wang","doi":"10.1016/j.bioelechem.2025.109169","DOIUrl":"10.1016/j.bioelechem.2025.109169","url":null,"abstract":"<div><div>The immobilization of enzymes is crucial for enhancing their catalytic activity and stability. Covalent organic frameworks (COF), with abundant active sites and tunable pore structures, enable effective immobilization of enzymes. Here, we designed carboxyl-functionalized COF (COF-COOH) to immobilize Cytochrome C (Cyt C), aiming to regulate the perfect pairing of the COF pore (3.67 nm) and the Cyt C dimension (2.6 nm × 3.2 nm × 3.3 nm). Meanwhile, the large amount of -COOH can increase the electrostatic and hydrogen bonding forces between COF-COOH and Cyt C. Thus, the Cyt C was efficiently loaded into COF-COOH through the post-modification method (loading efficiency = 62.37 %). The catalytic activity (<em>k</em><sub><em>cat</em></sub><em>/K</em><sub><em>m</em></sub>) of Cyt C@COF-COOH toward H<sub>2</sub>O<sub>2</sub> was significantly enhanced to 309.96 s<sup>−1</sup> M<sup>−1</sup> as compared to free Cyt C of 105.55 s<sup>−1</sup> M<sup>−1</sup>. The catalytic activity of Cyt C@COF-COOH toward H<sub>2</sub>O<sub>2</sub> still exceeds 80 % in some harsh environments (acetonitrile, dimethyl sulfoxide, tetrahydrofuran and 60 °C). The detection range of electrochemical H<sub>2</sub>O<sub>2</sub> biosensor based on Cyt C@COF-COOH is as wide as 2.0–80 μM, and the sensitivity is as high as 0.373 μA μM<sup>−1</sup> cm<sup>−2</sup>.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109169"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145555973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2025-09-23DOI: 10.1016/j.bioelechem.2025.109120
Jiaqing Wang , Youyu Li , Ning Li , Hongliang Han , Zhanfang Ma , Haijun Yang
Hydrogels are widely used in electrochemical sensors due to their unique properties, but their conductivity, influenced by water-content, is highly susceptible to external environment. Therefore, enhancing the water-retention of hydrogels while ensuring stable conductivity and analytical performance is crucial for broadening their application. In this work, a novel polyacrylamide/bacterial cellulose/sulfobetaine methacrylate/sodium alginate composite hydrogel (PBSS)-based multi-crosslinked network hydrogel was designed. The water retention of the PBSS hydrogel was improved by a factor of 1.5 compared to the unreinforced polyacrylamide (PAM) hydrogel (Water loss of hydrogel exposed for 12 h at 37 °C). With the water retention properties of itself, the PBSS hydrogel retained 86 % of its initial conductivity after 12 h of exposure at 60 °C, whereas the PAM hydrogel not only exhibited poor initial conductivity but also lost up to 47 % of its conductivity. PBSS hydrogels were designed as sensing platforms and CaCO3 spheres were designed as immunoprobes. Ca2+ released by the probe rivals Ni2+ for the signaling substance on the substrate, enabling the quantification of the target analyte. The sensor exhibited excellent analytical performance and maintained stable performance after four days of storage at 37 °C, offering a promising approach to enhance hydrogel sensor stability for clinical applications.
{"title":"Highly water-retaining conductive hydrogels based on multi-crosslinked networks for ultrasensitive sensing platform","authors":"Jiaqing Wang , Youyu Li , Ning Li , Hongliang Han , Zhanfang Ma , Haijun Yang","doi":"10.1016/j.bioelechem.2025.109120","DOIUrl":"10.1016/j.bioelechem.2025.109120","url":null,"abstract":"<div><div>Hydrogels are widely used in electrochemical sensors due to their unique properties, but their conductivity, influenced by water-content, is highly susceptible to external environment. Therefore, enhancing the water-retention of hydrogels while ensuring stable conductivity and analytical performance is crucial for broadening their application. In this work, a novel polyacrylamide/bacterial cellulose/sulfobetaine methacrylate/sodium alginate composite hydrogel (PBSS)-based multi-crosslinked network hydrogel was designed. The water retention of the PBSS hydrogel was improved by a factor of 1.5 compared to the unreinforced polyacrylamide (PAM) hydrogel (Water loss of hydrogel exposed for 12 h at 37 °C). With the water retention properties of itself, the PBSS hydrogel retained 86 % of its initial conductivity after 12 h of exposure at 60 °C, whereas the PAM hydrogel not only exhibited poor initial conductivity but also lost up to 47 % of its conductivity. PBSS hydrogels were designed as sensing platforms and CaCO<sub>3</sub> spheres were designed as immunoprobes. Ca<sup>2+</sup> released by the probe rivals Ni<sup>2+</sup> for the signaling substance on the substrate, enabling the quantification of the target analyte. The sensor exhibited excellent analytical performance and maintained stable performance after four days of storage at 37 °C, offering a promising approach to enhance hydrogel sensor stability for clinical applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109120"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2025-10-11DOI: 10.1016/j.bioelechem.2025.109135
Yuqiao Dong , Yuxuan Xu , Jinke Yin , Qianwen Jin , Guangzhou Liu
Biological damage remains a critical limiting factor that hinders the application of highly biocompatible titanium alloys in marine engineering. Exploiting the delicate electrostatic interactions at the interface between organisms and nanomaterials is of vital importance. In this study, an external direct current was applied to copper-coated capacitive TiO₂ nanotubes (TNT-Cu) to evaluate the antifouling effect on sessile biofilms and planktonic bacteria, and to elucidate the underlying mechanisms. The heterostructure exhibited high specific capacitance and superior antifouling performance. Upon electrochemical charge-discharge, TNT-Cu achieved 98.3 ± 0.9 % adhesion inhibition and 96.0 ± 2.0 % algicidal activity against Phaeodactylum tricornutum. The charged TNT-Cu achieved synergistic antifouling through surface charge–induced electrostatic sterilization and controlled Cu2+ release. Physiologically, the electrical interaction combined with Cu2+ significantly disrupted algal electron transport, induced reactive oxygen species (ROS) accumulation, and caused membrane rupture. This work provides a promising, durable, and eco-friendly antifouling strategy for marine applications of titanium-based materials.
{"title":"Electroactive TiO₂–Cu nanotube platform with synergistic charge modulation and Cu2+ release for marine antifouling","authors":"Yuqiao Dong , Yuxuan Xu , Jinke Yin , Qianwen Jin , Guangzhou Liu","doi":"10.1016/j.bioelechem.2025.109135","DOIUrl":"10.1016/j.bioelechem.2025.109135","url":null,"abstract":"<div><div>Biological damage remains a critical limiting factor that hinders the application of highly biocompatible titanium alloys in marine engineering. Exploiting the delicate electrostatic interactions at the interface between organisms and nanomaterials is of vital importance. In this study, an external direct current was applied to copper-coated capacitive TiO₂ nanotubes (TNT-Cu) to evaluate the antifouling effect on sessile biofilms and planktonic bacteria, and to elucidate the underlying mechanisms. The heterostructure exhibited high specific capacitance and superior antifouling performance. Upon electrochemical charge-discharge, TNT-Cu achieved 98.3 ± 0.9 % adhesion inhibition and 96.0 ± 2.0 % algicidal activity against <em>Phaeodactylum tricornutum</em>. The charged TNT-Cu achieved synergistic antifouling through surface charge–induced electrostatic sterilization and controlled Cu<sup>2+</sup> release. Physiologically, the electrical interaction combined with Cu<sup>2+</sup> significantly disrupted algal electron transport, induced reactive oxygen species (ROS) accumulation, and caused membrane rupture. This work provides a promising, durable, and eco-friendly antifouling strategy for marine applications of titanium-based materials.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109135"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2025-10-18DOI: 10.1016/j.bioelechem.2025.109145
Dongcheng Yang , Huan Wang , Caiyu Wang , Xinyu Liu , Lihua Hu , Hongmin Ma , Dan Wu , Xiang Ren , Yuyang Li , Qin Wei
Electrochemiluminescence (ECL) technology has become an essential analytical methodology in biomolecular detection, attaining both profound research advancements and extensive practical applications in this domain. Herein, we used carboxylated mesoporous silica (MSN-COOH) as a container to encapsulate Tris(2,2-bipyridyl)ruthenium(II)2+ (Ru(bpy)32+)—a luminophore—in its pores to achieve enrichment (RuMSN). The reactive intermediates Ru(bpy)33+ and TPrA•+ are attracted by the electro-negative carboxyl group on the MSN-COOH surface, substantially reducing the reaction distance and improving the ECL response efficiency. The flower-like NiFe layered double hydroxide (NiFe-LDH) nanostructure possesses significantly high specific surface area and good electrocatalytic performance, while gold nanoparticles also demonstrate excellent electrical conductivity and biocompatibility. To enhance sensor sensitivity, gold nanoparticles were loaded onto the NiFe-LDH surface forming the Au@NiFe-LDH composite, which improves performance by enhancing conductive properties and increasing antigen-antibody binding sites. This study established a sandwich-configuration electrochemiluminescence detection platform capable of highly sensitive PSA analysis.The biosensor demonstrated excellent stability, specificity and selectivity, with a linear detection range of 0.1 pg/mL-50 ng/mL and a detection limit as low as 63 fg/mL (S/N = 3). This confirms the clinical application value of the detection system in the early screening of prostate cancer.
{"title":"Ultrasensitive electrochemiluminescence immunosensor based on gold-functionalised NiFe layered double hydroxide and electro-negative mesoporous silica for prostate-specific antigen detection","authors":"Dongcheng Yang , Huan Wang , Caiyu Wang , Xinyu Liu , Lihua Hu , Hongmin Ma , Dan Wu , Xiang Ren , Yuyang Li , Qin Wei","doi":"10.1016/j.bioelechem.2025.109145","DOIUrl":"10.1016/j.bioelechem.2025.109145","url":null,"abstract":"<div><div>Electrochemiluminescence (ECL) technology has become an essential analytical methodology in biomolecular detection, attaining both profound research advancements and extensive practical applications in this domain. Herein, we used carboxylated mesoporous silica (MSN-COOH) as a container to encapsulate Tris(2,2-bipyridyl)ruthenium(II)<sup>2+</sup> (Ru(bpy)<sub>3</sub><sup>2+</sup>)—a luminophore—in its pores to achieve enrichment (RuMSN). The reactive intermediates Ru(bpy)<sub>3</sub><sup>3+</sup> and TPrA<sup>•+</sup> are attracted by the electro-negative carboxyl group on the MSN-COOH surface, substantially reducing the reaction distance and improving the ECL response efficiency. The flower-like Ni<img>Fe layered double hydroxide (NiFe-LDH) nanostructure possesses significantly high specific surface area and good electrocatalytic performance, while gold nanoparticles also demonstrate excellent electrical conductivity and biocompatibility. To enhance sensor sensitivity, gold nanoparticles were loaded onto the NiFe-LDH surface forming the Au@NiFe-LDH composite, which improves performance by enhancing conductive properties and increasing antigen-antibody binding sites. This study established a sandwich-configuration electrochemiluminescence detection platform capable of highly sensitive PSA analysis.The biosensor demonstrated excellent stability, specificity and selectivity, with a linear detection range of 0.1 pg/mL-50 ng/mL and a detection limit as low as 63 fg/mL (S/N = 3). This confirms the clinical application value of the detection system in the early screening of prostate cancer.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109145"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145342478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2025-09-09DOI: 10.1016/j.bioelechem.2025.109106
Namrata Upadhyay , Sudhir K. Shukla , N. Malathy , Y.V. Nancharaiah , A. Ravi Shankar , S. Ningshen
This study investigates microbiologically-influenced corrosion (MIC) aspects of borated and non-borated 304 L- stainless-steel using Bacillus subtilis SNF-1, which was isolated from the spent nuclear fuel pool (SNF). Over 28 days, electrochemical analyses revealed distinct corrosion behaviours: borated 304 L SS exhibited a more pronounced decrease in open circuit potential (from 0.03 to −0.35 V vs. Ag/AgCl) as compared to non-borated 304 L SS (from 0.05 to −0.10 V vs. Ag/AgCl) indicating higher susceptibility to MIC. Potentiodynamic polarization studies revealed an increase in passive current density (from 1.5 to 2.4 μA.cm−2 for non-borated 304 L SS and from 2.4 to 3.4 μA.cm−2 for borated 304 L SS), along with a lower pitting potential indicating the role of B. subtilis SNF-1 in MIC. Electrochemical impedance spectroscopy confirmed accelerated degradation, with polarization resistance (Rp) dropping by 69 % in borated 304 L SS and 86 % in non-borated 304 L SS. Despite higher absolute corrosion rates in borated 304 L SS, non-borated 304 L SS experienced a greater relative increase in corrosion (3.8-fold vs. 2.3-fold) due to denser biofilm coverage (95 % vs. 74 %). Surface analysis identified localized pitting beneath biofilms, exacerbated by boride-induced micro-galvanic effects. These findings underscore the dual role of alloy microstructure and biofilm dynamics in MIC severity.
本研究利用从乏燃料池（SNF）中分离出来的枯草芽孢杆菌SNF-1研究了硼化和非硼化304 L不锈钢的微生物影响腐蚀（MIC）问题。在28天的时间里，电化学分析显示了不同的腐蚀行为：与未含硼的304 L SS（从0.05到- 0.10 V）相比，含硼的304 L SS的开路电位（从0.03到- 0.35 V vs Ag/AgCl）下降更为明显，表明对MIC的敏感性更高。动电位极化研究表明，无源电流密度增加（未加硼的304 L SS从1.5 μA.cm−2增加到2.4 μA.cm−2，加硼的304 L SS从2.4 μA.cm−2增加到3.4 μA.cm−2），同时点蚀电位降低，表明枯草芽孢杆菌SNF-1在MIC中的作用。电化学阻抗谱证实了加速降解，含硼304 L SS的极化电阻（Rp）下降了69%，未含硼304 L SS的极化电阻（Rp）下降了86%。尽管含硼304 L SS的绝对腐蚀速率更高，但由于生物膜覆盖率更高（95%对74%），未含硼304 L SS的腐蚀相对增加更大（3.8倍对2.3倍）。表面分析确定了生物膜下的局部点蚀，由硼化物引起的微电效应加剧。这些发现强调了合金微观结构和生物膜动力学在MIC严重程度中的双重作用。
{"title":"Biocorrosion studies on borated and non-borated 304 L stainless steel using Bacillus subtilis SNF-1, a bacterial isolate from SNF pool","authors":"Namrata Upadhyay , Sudhir K. Shukla , N. Malathy , Y.V. Nancharaiah , A. Ravi Shankar , S. Ningshen","doi":"10.1016/j.bioelechem.2025.109106","DOIUrl":"10.1016/j.bioelechem.2025.109106","url":null,"abstract":"<div><div>This study investigates microbiologically-influenced corrosion (MIC) aspects of borated and non-borated 304 L- stainless-steel using <em>Bacillus subtilis</em> SNF-1, which was isolated from the spent nuclear fuel pool (SNF). Over 28 days, electrochemical analyses revealed distinct corrosion behaviours: borated 304 L SS exhibited a more pronounced decrease in open circuit potential (from 0.03 to −0.35 V vs. Ag/AgCl) as compared to non-borated 304 L SS (from 0.05 to −0.10 V vs. Ag/AgCl) indicating higher susceptibility to MIC. Potentiodynamic polarization studies revealed an increase in passive current density (from 1.5 to 2.4 μA.cm<sup>−2</sup> for non-borated 304 L SS and from 2.4 to 3.4 μA.cm<sup>−2</sup> for borated 304 L SS), along with a lower pitting potential indicating the role of <em>B. subtilis</em> SNF-1 in MIC. Electrochemical impedance spectroscopy confirmed accelerated degradation, with polarization resistance (R<sub>p</sub>) dropping by 69 % in borated 304 L SS and 86 % in non-borated 304 L SS. Despite higher absolute corrosion rates in borated 304 L SS, non-borated 304 L SS experienced a greater relative increase in corrosion (3.8-fold vs. 2.3-fold) due to denser biofilm coverage (95 % vs. 74 %). Surface analysis identified localized pitting beneath biofilms, exacerbated by boride-induced micro-galvanic effects. These findings underscore the dual role of alloy microstructure and biofilm dynamics in MIC severity.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109106"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The twin microbial fuel cell powered electro-Fenton system (twin-MFCⓅEFs), combining active oxygen component and microbial metabolism, was constructed to improve the treatment process of waste activated sludge (WAS). Nevertheless, the performance and mechanism of electron transfer underlying this enhancement remain poorly understood. This study investigated the performance and mechanism of electron generation and utilization in twin-MFCⓅEFs with WAS as substrate. The higher electron generation and recovery efficiency (8.25 % of coulombic efficiency) was attributed to the higher content of amino acids (such as tryptophan), humic substances and their aromatic groups and unsaturated conjugated double bonds in the soluble organic matter, which facilitated biodegradation and electron transfer. The higher electron utilization performance (52.76 % of faraday efficiency) relied on the superior electron supply system that exhibited greater free radical oxidation. Metagenomic analysis indicated that an increased secretory capacity of glycosyltransferases (including glucosyltransferases and β-glucosidases) and a reduced activity of acetate kinase and methyl-coenzyme M reductase alpha subunit in cellular metabolic processes favored signaling and electricity production. The study focused on electron flow in twin-MFCⓅEFs and offered a promising strategy for improving the sludge treatment process.
{"title":"Electron transfer performance and mechanism in twin microbial fuel cell powered electro-Fenton system with waste activated sludge as substrate","authors":"Jiaqi Lv , Qingliang Zhao , Junqiu Jiang , Jing Ding , Liangliang Wei , Jiawen Liang","doi":"10.1016/j.bioelechem.2025.109154","DOIUrl":"10.1016/j.bioelechem.2025.109154","url":null,"abstract":"<div><div>The twin microbial fuel cell powered electro-Fenton system (twin-MFCⓅEFs), combining active oxygen component and microbial metabolism, was constructed to improve the treatment process of waste activated sludge (WAS). Nevertheless, the performance and mechanism of electron transfer underlying this enhancement remain poorly understood. This study investigated the performance and mechanism of electron generation and utilization in twin-MFCⓅEFs with WAS as substrate. The higher electron generation and recovery efficiency (8.25 % of coulombic efficiency) was attributed to the higher content of amino acids (such as tryptophan), humic substances and their aromatic groups and unsaturated conjugated double bonds in the soluble organic matter, which facilitated biodegradation and electron transfer. The higher electron utilization performance (52.76 % of faraday efficiency) relied on the superior electron supply system that exhibited greater free radical oxidation. Metagenomic analysis indicated that an increased secretory capacity of glycosyltransferases (including glucosyltransferases and β-glucosidases) and a reduced activity of acetate kinase and methyl-coenzyme M reductase alpha subunit in cellular metabolic processes favored signaling and electricity production. The study focused on electron flow in twin-MFCⓅEFs and offered a promising strategy for improving the sludge treatment process.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109154"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145385494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2025-10-28DOI: 10.1016/j.bioelechem.2025.109152
Julia Czopinska , Filip Budny , Andrzej Peplowski , Anna Sobiepanek , Marta Jarczewska
Carbon – based surfaces are becoming attractive alternative to gold transducers, which are commonly applied for development of nucleic acid sensors. However, one of the challenges is the necessity of elaboration of carbon transducers modification methods, yielding stable receptor layers that are capable of binding to target analytes. Herein, we present the studies on the elaboration of aptamer layers aimed for electrochemical detection of cytokine - tumor necrosis factor-alpha (TNF-α). This protein can serve as biomarker of wound healing processes that might be the answer to need for rapid determination of the stage of the wound that can result in selection of proper diagnosis as well as treatment strategy. We aimed on functionalization of edge – plane pyrolytic graphite as well as planar screen – printed electrodes. The aptamer layers were formed through one-step procedure using pyrene and anthracene anchor groups conjugated with aptamers. The research enabled to choose ferri/ferrocyanide redox indicator that was the source of electrochemical signal, determine the range of linear response (5–200 ng/L) with LOD of 5.07 ng/L and test the sensor against complex samples of serum or simulated wound exudate. The developed aptasensor proved to be promising solution towards non-invasive monitoring of wound healing.
{"title":"Application of aptamer – carbon hybrid materials for electrochemical detection of wound healing biomarker – TNF-α protein","authors":"Julia Czopinska , Filip Budny , Andrzej Peplowski , Anna Sobiepanek , Marta Jarczewska","doi":"10.1016/j.bioelechem.2025.109152","DOIUrl":"10.1016/j.bioelechem.2025.109152","url":null,"abstract":"<div><div>Carbon – based surfaces are becoming attractive alternative to gold transducers, which are commonly applied for development of nucleic acid sensors. However, one of the challenges is the necessity of elaboration of carbon transducers modification methods, yielding stable receptor layers that are capable of binding to target analytes. Herein, we present the studies on the elaboration of aptamer layers aimed for electrochemical detection of cytokine - tumor necrosis factor-alpha (TNF-α). This protein can serve as biomarker of wound healing processes that might be the answer to need for rapid determination of the stage of the wound that can result in selection of proper diagnosis as well as treatment strategy. We aimed on functionalization of edge – plane pyrolytic graphite as well as planar screen – printed electrodes. The aptamer layers were formed through one-step procedure using pyrene and anthracene anchor groups conjugated with aptamers. The research enabled to choose ferri/ferrocyanide redox indicator that was the source of electrochemical signal, determine the range of linear response (5–200 ng/L) with LOD of 5.07 ng/L and test the sensor against complex samples of serum or simulated wound exudate. The developed aptasensor proved to be promising solution towards non-invasive monitoring of wound healing.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109152"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2025-09-19DOI: 10.1016/j.bioelechem.2025.109112
Gulam Rabbani , Akbar Mohammad , Mohammad Ehtisham Khan , Waleed Zakri , Mohsin Vahid Khan , Khurshid Ahamd , Wahid Ali , Syed Kashif Ali , Nazim Hasan , Abdulrahman Khamaj , Jintae Lee
In this study, alpha-1-acid glycoprotein (AGP), a crucial biomarker associated with various diseases and physiological conditions, was selected for detection using the developed immunosensor. The immunosensor was fabricated by depositing a synthesized Nafion/silver nanoparticles (AgNPs) nanocomposite on the glassy carbon electrode as the substrate material. The changes in the physicochemical properties after Nafion/AgNPs nanocomposite deposition were analyzed through SEM and XPS measurements. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to analyze the immunosensor fabrication's electrochemical performance. AGP was detected through the immobilized anti-AGP on the electrode surface. The developed immunosensor exhibited a wide linear detection range from 0.05 to 3.2 mg/mL and a LOD of 0.17 mg/mL. The developed immunosensor showed excellent selectivity and repeatability when subjected to different kinds of interfering proteins. The recovery of AGP ranged from 101.7 to 103.7 % and RSD was <4 %, indicating high accuracy in real samples detection. The immunosensor developed for AGP detection showed a number of beneficial characteristics, including low cost and small volume sample requirements, making it highly suitable for point-of-care applications. This study provides new insights into the precise fabrication and affordable immunosensors for diverse clinical diagnostic applications.
{"title":"Nafion-stabilized silver nanoparticles modified glassy carbon electrode for ultrasensitive detection of alpha-1-acid glycoprotein","authors":"Gulam Rabbani , Akbar Mohammad , Mohammad Ehtisham Khan , Waleed Zakri , Mohsin Vahid Khan , Khurshid Ahamd , Wahid Ali , Syed Kashif Ali , Nazim Hasan , Abdulrahman Khamaj , Jintae Lee","doi":"10.1016/j.bioelechem.2025.109112","DOIUrl":"10.1016/j.bioelechem.2025.109112","url":null,"abstract":"<div><div>In this study, alpha-1-acid glycoprotein (AGP), a crucial biomarker associated with various diseases and physiological conditions, was selected for detection using the developed immunosensor. The immunosensor was fabricated by depositing a synthesized Nafion/silver nanoparticles (AgNPs) nanocomposite on the glassy carbon electrode as the substrate material. The changes in the physicochemical properties after Nafion/AgNPs nanocomposite deposition were analyzed through SEM and XPS measurements. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to analyze the immunosensor fabrication's electrochemical performance. AGP was detected through the immobilized anti-AGP on the electrode surface. The developed immunosensor exhibited a wide linear detection range from 0.05 to 3.2 mg/mL and a LOD of 0.17 mg/mL. The developed immunosensor showed excellent selectivity and repeatability when subjected to different kinds of interfering proteins. The recovery of AGP ranged from 101.7 to 103.7 % and RSD was <4 %, indicating high accuracy in real samples detection. The immunosensor developed for AGP detection showed a number of beneficial characteristics, including low cost and small volume sample requirements, making it highly suitable for point-of-care applications. This study provides new insights into the precise fabrication and affordable immunosensors for diverse clinical diagnostic applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109112"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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