Bioelectrochemistry最新文献_第10页

Highly water-retaining conductive hydrogels based on multi-crosslinked networks for ultrasensitive sensing platform 基于多交联网络的高保水导电水凝胶用于超灵敏传感平台

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-23 DOI: 10.1016/j.bioelechem.2025.109120

Jiaqing Wang , Youyu Li , Ning Li , Hongliang Han , Zhanfang Ma , Haijun Yang

Hydrogels are widely used in electrochemical sensors due to their unique properties, but their conductivity, influenced by water-content, is highly susceptible to external environment. Therefore, enhancing the water-retention of hydrogels while ensuring stable conductivity and analytical performance is crucial for broadening their application. In this work, a novel polyacrylamide/bacterial cellulose/sulfobetaine methacrylate/sodium alginate composite hydrogel (PBSS)-based multi-crosslinked network hydrogel was designed. The water retention of the PBSS hydrogel was improved by a factor of 1.5 compared to the unreinforced polyacrylamide (PAM) hydrogel (Water loss of hydrogel exposed for 12 h at 37 °C). With the water retention properties of itself, the PBSS hydrogel retained 86 % of its initial conductivity after 12 h of exposure at 60 °C, whereas the PAM hydrogel not only exhibited poor initial conductivity but also lost up to 47 % of its conductivity. PBSS hydrogels were designed as sensing platforms and CaCO₃ spheres were designed as immunoprobes. Ca²⁺ released by the probe rivals Ni²⁺ for the signaling substance on the substrate, enabling the quantification of the target analyte. The sensor exhibited excellent analytical performance and maintained stable performance after four days of storage at 37 °C, offering a promising approach to enhance hydrogel sensor stability for clinical applications.

水凝胶因其独特的性能被广泛应用于电化学传感器中，但其电导率受水含量的影响，极易受外界环境的影响。因此，在保证稳定的电导率和分析性能的同时提高水凝胶的保水性是扩大其应用的关键。本文设计了一种新型的聚丙烯酰胺/细菌纤维素/甲基丙烯酸磺基甜菜碱/海藻酸钠复合水凝胶（PBSS）。与未增强的聚丙烯酰胺（PAM）水凝胶相比，PBSS水凝胶的保水性提高了1.5倍（水凝胶在37℃下暴露12小时的失水）。由于PBSS水凝胶本身具有保水性，在60°C下暴露12小时后，其初始电导率仍保持86%，而PAM水凝胶不仅表现出较差的初始电导率，而且电导率损失高达47%。设计PBSS水凝胶作为传感平台，CaCO3球作为免疫探针。探针释放的Ca2+与底物上的信号物质Ni2+竞争，使目标分析物的量化成为可能。该传感器表现出优异的分析性能，并在37°C下保存4天后保持稳定的性能，为提高水凝胶传感器的稳定性提供了一种有前途的方法。

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引用次数: 0

A colorimetric/electrochemical dual-mode aptasensor for Golgi protein 73 detection using TMB as a bifunctional probe 以TMB为双功能探针的高尔基蛋白73检测比色/电化学双模适体传感器

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-23 DOI: 10.1016/j.bioelechem.2025.109121

Guiyin Li , Jing Su , Min Chen , Xiaohong Tan , Lingling Fan , Zhide Zhou , Chong Hu , Jintao Liang

Golgi protein 73 (GP73) has emerged as a critical biomarker for the diagnosis of hepatocellular carcinoma (HCC), and its detection is essential for the effective treatment of this disease. In this study, we developed a colorimetric/electrochemical dual-mode aptasensor for GP73 detection using a hemin-reduced graphene oxide‑manganese oxide (H-rGO-Mn₃O₄) nanozyme and the bifunctional probe, 3,3′,5,5′-tetramethylbenzidine (TMB). The GP73-specific aptamer (Apt) was conjugated to the H-rGO-Mn₃O₄ nanozyme, which was then immobilized on the surface of a gold nanoparticle-modified screen-printed electrode (Au NPs/SPE) through hybridization with a complementary DNA strand (cDNA) attached to the electrode. TMB served as a dual-signal probe for both colorimetric and electrochemical detection. In the presence of GP73, Apt binds specifically to the target, forming a H-rGO-Mn₃O₄-Apt/GP73 complex structure. This binding event causes the nanozymes to detach from the electrode surface, reducing its peroxidase activity while simultaneously restoring the electrochemical signal. Under optimized conditions, the dual-mode aptasensor exhibited excellent linear responses for GP73 concentrations ranging from 0.0001 to 100 ng/mL, with limits of detection (LOD) of 0.1 pg/mL for both the colorimetric and electrochemical modes. The aptasensor was successfully applied to measure GP73 levels in serum samples, yielding highly consistent results. Its performance was further validated by comparison with the standard ELISA method. Compared to single-signal aptasensors, this dual-mode approach not only improves detection sensitivity but also minimizes the risk of false positives or false negatives, making it a more reliable tool for accurate GP73 detection in clinical diagnostics.

高尔基蛋白73 （GP73）已成为肝细胞癌（HCC）诊断的重要生物标志物，其检测对于有效治疗这种疾病至关重要。在这项研究中，我们开发了一种用于GP73检测的比色/电化学双模式传感器，该传感器使用血红素还原氧化石墨烯-氧化锰（H-rGO-Mn3O4）纳米酶和双功能探针3,3 ',5,5 ' -四甲基联苯胺（TMB）。将gp73特异性适配体（Apt）与H-rGO-Mn3O4纳米酶偶联，然后通过与电极上的互补DNA链（cDNA）杂交，将其固定在金纳米颗粒修饰的丝网印刷电极（Au NPs/SPE）表面。TMB作为一种双信号探针，可用于比色和电化学检测。在GP73存在的情况下，Apt特异性结合靶标，形成H-rGO-Mn3O4-Apt/GP73复合物结构。这一结合事件导致纳米酶从电极表面分离，降低其过氧化物酶活性，同时恢复电化学信号。在优化条件下，双模传感器对GP73浓度在0.0001 ~ 100 ng/mL范围内表现出良好的线性响应，比色和电化学模式的检出限（LOD）均为0.1 pg/mL。该适配体传感器成功应用于血清样品中GP73水平的测量，结果高度一致。通过与标准ELISA法的比较，进一步验证了其性能。与单信号适配传感器相比，这种双模式方法不仅提高了检测灵敏度，而且最大限度地降低了假阳性或假阴性的风险，使其成为临床诊断中准确检测GP73的更可靠工具。

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引用次数: 0

Electrolytically generated size-tunable micro-nano bubbles toward high-efficiency antibacterial applications in vitro 电解生成尺寸可调微纳气泡的体外高效抗菌应用

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-19 DOI: 10.1016/j.bioelechem.2025.109117

Qiang Mao , Kai Wei , Tianyu Li , Jiasheng Zhu , Huarui Han , Kan Hu , Changchang Ma , Sheng Feng

Bacterial contamination in water poses severe threats to global water safety, particularly in drinking water. Micro-nano bubbles (MNBs) have attracted significant interest for antibacterial applications due to their high efficiency and environmental friendliness. In this study, size-tunable MNBs were electrochemically fabricated using TiO₂ nanotube arrays (TNTAs) as electrodes. A strong positive correlation (R² = 0.965) was established between the pore diameter of TNTAs and the size of the generated MNBs, enabling precise control over bubble dimensions. Crucially, bactericidal efficiency was found to be negatively correlated with MNB size, with the smallest MNBs achieving exceptional inactivation rates of 98.8 % for Escherichia coli (E. coli) and 98.0 % for Staphylococcus aureus (S. aureus). Mechanistic studies revealed that smaller MNBs generate higher concentrations of hydroxyl radicals (•OH) upon collapse, which are primarily responsible for the enhanced antibacterial activity. This work establishes a clear structure–activity relationship: electrode pore size dictates MNB size, which in turn determines •OH generation and antibacterial performance. These findings provide a scientific basis for developing efficient, environmentally friendly disinfection technologies.

水中的细菌污染对全球水安全，特别是饮用水安全构成严重威胁。微纳气泡（MNBs）因其高效、环保的特点在抗菌领域引起了广泛的关注。在本研究中，采用TiO2纳米管阵列（TNTAs）作为电极，电化学制备了尺寸可调的mnb。tnta的孔径与生成的mnb的大小之间存在很强的正相关关系（R2 = 0.965），可以精确控制气泡的大小。至关重要的是，研究发现杀菌效率与MNB大小呈负相关，最小的MNB对大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）的失活率分别为98.8%和98.0%。机制研究表明，较小的MNBs在崩溃时产生更高浓度的羟基自由基（•OH），这是增强抗菌活性的主要原因。这项工作建立了一个明确的结构-活性关系：电极孔径决定了MNB的大小，这反过来又决定了•OH的生成和抗菌性能。这些发现为开发高效、环保的消毒技术提供了科学依据。

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引用次数: 0

Nafion-stabilized silver nanoparticles modified glassy carbon electrode for ultrasensitive detection of alpha-1-acid glycoprotein 钠稳定的纳米银修饰玻碳电极用于α -1-酸性糖蛋白的超灵敏检测

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-19 DOI: 10.1016/j.bioelechem.2025.109112

Gulam Rabbani , Akbar Mohammad , Mohammad Ehtisham Khan , Waleed Zakri , Mohsin Vahid Khan , Khurshid Ahamd , Wahid Ali , Syed Kashif Ali , Nazim Hasan , Abdulrahman Khamaj , Jintae Lee

In this study, alpha-1-acid glycoprotein (AGP), a crucial biomarker associated with various diseases and physiological conditions, was selected for detection using the developed immunosensor. The immunosensor was fabricated by depositing a synthesized Nafion/silver nanoparticles (AgNPs) nanocomposite on the glassy carbon electrode as the substrate material. The changes in the physicochemical properties after Nafion/AgNPs nanocomposite deposition were analyzed through SEM and XPS measurements. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to analyze the immunosensor fabrication's electrochemical performance. AGP was detected through the immobilized anti-AGP on the electrode surface. The developed immunosensor exhibited a wide linear detection range from 0.05 to 3.2 mg/mL and a LOD of 0.17 mg/mL. The developed immunosensor showed excellent selectivity and repeatability when subjected to different kinds of interfering proteins. The recovery of AGP ranged from 101.7 to 103.7 % and RSD was <4 %, indicating high accuracy in real samples detection. The immunosensor developed for AGP detection showed a number of beneficial characteristics, including low cost and small volume sample requirements, making it highly suitable for point-of-care applications. This study provides new insights into the precise fabrication and affordable immunosensors for diverse clinical diagnostic applications.

本研究选择与多种疾病和生理状况相关的重要生物标志物α -1-酸性糖蛋白（AGP）进行检测。将合成的纳米银纳米复合材料（AgNPs）沉积在玻碳电极上作为衬底材料制备免疫传感器。通过SEM和XPS测试分析了纳米复合材料沉积后理化性质的变化。利用循环伏安法（CV）和电化学阻抗谱法（EIS）分析了免疫传感器的电化学性能。AGP通过固定在电极表面的抗AGP检测。该免疫传感器的线性检测范围为0.05 ~ 3.2 mg/mL， LOD为0.17 mg/mL。所研制的免疫传感器对不同种类的干扰蛋白具有良好的选择性和重复性。AGP的回收率为101.7% ~ 103.7%，RSD为4%，对实际样品的检测具有较高的准确性。用于AGP检测的免疫传感器显示出许多有益的特性，包括低成本和小体积样品要求，使其非常适合于护理点应用。这项研究为精确制造和负担得起的免疫传感器提供了新的见解，用于各种临床诊断应用。

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引用次数: 0

An electrochemical aptasensor based on synergistic effect of duplex-specific nuclease and nanoporous gold for ultrasensitive detection of prostate-specific antigen 基于双特异性核酸酶和纳米孔金协同作用的电化学配体传感器用于前列腺特异性抗原的超灵敏检测

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-19 DOI: 10.1016/j.bioelechem.2025.109113

Xiaohui Wang , Xinran Li , Yanxia Xie , Hongshuai Fang , Xueru Cui , Jianwei Jiao , Yachao Zhang

Prostate cancer (PCa), the most common solid tumor in men, is typically suspected based on digital rectal examination and elevated serum prostate-specific antigen (PSA) levels. Given its clinical significance, developing a PSA biosensing system with high sensitivity and stability is essential. In this study, a novel aptasensor, leveraging the synergistic effect of duplex specific nuclease (DSN) and nanoporous gold (NP-Gold), was firstly developed for PSA detection. The aptamer, which hybridizes with its complementary DNA to form double-stranded DNA (dsDNA), was used as biorecognition element. NP-Gold served as the supporting material. By exploiting the large specific surface area and high conductivity of NP-Gold, the immobilized amount of dsDNA was increased, thereby expanding the dynamic detection range of PSA. DSN can recognize and cleave non-PSA-bound dsDNA. By reducing noise signals derived from such unbound dsDNA, it enhances the correlation between electrical signals and PSA concentration, ultimately lowering the detection limit. Benefiting from the synergistic effect of DSN and NP-Gold, the developed aptasensor showed a wide linear relationship between current values and PSA concentrations, with a detection range from 100 fg/mL to 10 ng/mL and a limit of detection (LOD) of 2.7 fg/mL. Moreover, the aptasensor demonstrated excellent resistance to interference from various proteins and retained 96 % of its current response after 21 days of storage. Additionally, the proposed aptasensor successfully detected PSA concentrations in real human serum samples, with accuracy comparable to that of the ELISA kit. These distinctive characteristics made the aptasensor a reliable choice for PSA detection and early diagnosis of PCa.

前列腺癌（PCa）是男性最常见的实体肿瘤，通常根据直肠指检和血清前列腺特异性抗原（PSA）水平升高来怀疑。鉴于其临床意义，开发一种高灵敏度、高稳定性的PSA生物传感系统至关重要。在这项研究中，利用双特异性核酸酶（DSN）和纳米孔金（NP-Gold）的协同作用，首次开发了一种用于PSA检测的新型适配体传感器。该适体与其互补DNA杂交形成双链DNA (dsDNA)，被用作生物识别元件。NP-Gold作为支撑材料。利用NP-Gold的大比表面积和高导电性，增加了dsDNA的固定化量，从而扩大了PSA的动态检测范围。DSN可以识别和切割非psa结合的dsDNA。通过降低来自这些未结合dsDNA的噪声信号，增强电信号与PSA浓度之间的相关性，最终降低检测限。利用DSN和NP-Gold的协同效应，该传感器的检测范围为100 ~ 10 ng/mL，检出限（LOD）为2.7 fg/mL，电流值与PSA浓度之间存在广泛的线性关系。此外，该配体传感器对各种蛋白质的干扰表现出优异的抵抗力，并在21天后保持了96%的当前响应。此外，所提出的适配体传感器成功地检测了真实人血清样品中的PSA浓度，其准确性与ELISA试剂盒相当。这些独特的特征使该传感器成为PSA检测和早期诊断前列腺癌的可靠选择。

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引用次数: 0

Integrated methane microbial fuel cell system for concurrent nitrogen and sulfur removal through autotrophic cathodic bioreduction 通过自养阴极生物还原同时去除氮和硫的集成甲烷微生物燃料电池系统

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-19 DOI: 10.1016/j.bioelechem.2025.109116

Jing Lu, Xinyu Li, Yating Jia, Liuyi Cai, Xinrui Wu, Shuqing Zheng, Bin Hou

The cathodic reduction of nitrate and sulfate by autotrophic organisms in microbial fuel cells (MFC) has received much attention. However, systematic investigations into the feasibility of methane-driven nitrate and sulfate reduction remain limited. In this study, an MFC integrating an autotrophic biocathode with dissolved methane (CH₄) as the electron donor was developed to couple methane-driven reduction of nitrate and sulfate with electricity generation. The biology reductive properties, microbial characteristics and functional metabolic mechanisms were investigated under single electron acceptor (NO₃⁻/SO₄²⁻) and mixed electron acceptor (NO₃⁻ + SO₄²⁻) working conditions. The results showed that with nitrate and sulfate acting as electron acceptors alone, MFC achieved the maximum removal rates of nitrate and sulfate of 89.1 % and 26 %, respectively. With mixed electron acceptors provided, the removal rates of nitrate and sulfate decreased by 62.5 % and 14.2 %, respectively. However, the anaerobic oxidation of methane (AOM) was promoted, and its output voltage reached a maximum. The anode chambers of all methane autotrophic denitrification‑sulfur removing MFCs shared similar microbial structures, with dominant functional genera including Methylocystis, Hyphomicrobium and Methylomonas, and the dominant bacteria in the cathode chamber were Pseudomonas, Nitrospira, Desulfovibrio, Hyphomicrobium and Acidovorax. The genes coding for methane metabolism were upregulated when nitrate and sulfate coexisted, while the genes related to sulfur metabolism and denitrification metabolism were downregulated. These findings provide novel insights into the application of AOM-MFC systems for the treatment of wastewater with nitrogen and sulfur contaminants.

微生物燃料电池（MFC）中自养生物阴极还原硝酸盐和硫酸盐的研究受到了广泛关注。然而，对甲烷驱动的硝酸盐和硫酸盐还原可行性的系统研究仍然有限。在本研究中，开发了一种以溶解甲烷（CH4）为电子供体的自养生物阴极MFC，将甲烷驱动的硝酸盐和硫酸盐还原与发电结合起来。研究了单电子受体（NO3−/SO42−）和混合电子受体（NO3−+ SO42−）条件下的生物还原特性、微生物特性和功能代谢机制。结果表明，当硝酸盐和硫酸盐单独作为电子受体时，MFC对硝酸盐和硫酸盐的去除率最高，分别为89.1%和26%。混合电子受体对硝酸盐和硫酸盐的去除率分别降低62.5%和14.2%。然而，甲烷（AOM）的厌氧氧化得到了促进，其输出电压达到了最大值。所有甲烷自养反硝化-脱硫mfc的阳极室微生物结构相似，优势功能属为Methylocystis、Hyphomicrobium和Methylomonas，阴极室优势菌为Pseudomonas、Nitrospira、Desulfovibrio、Hyphomicrobium和Acidovorax。硝酸盐和硫酸盐共存时，甲烷代谢基因表达上调，硫代谢和反硝化代谢基因表达下调。这些发现为AOM-MFC系统在处理含氮和硫污染物废水中的应用提供了新的见解。

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引用次数: 0

A new strategy for assessing chemo cardiotoxicity risk based on CRP recognition and the application of novel sensing technology 基于CRP识别和新型传感技术应用的化疗心脏毒性风险评估新策略

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-18 DOI: 10.1016/j.bioelechem.2025.109110

Bolu Sun , Haiying He , Zixia Wang , Yuqiong Wu , Jiali Kang , Xinlan Wang , Xuanxiu Da , Miao Zhou , Ying Lv , Chenyu Qin , Xiangdong Wang , Lin Yang , Huajie Li , Hai Wang

Cancer chemotherapy, while enhancing patient survival, is limited by cardiotoxicity. Early monitoring and risk assessment are essential. C-reactive protein (CRP) can serve as an early biomarker for predicting cardiotoxicity during chemotherapy, offering a critical opportunity to develop new cardioprotective strategies. This study used an anti-CRP antibody loaded onto an electrode modified with chitosan (CS) and functionalized carbon black (f-CB) as a target. Due to f-CB's excellent electrical conductivity and CS's high permeability and strong adhesion, an immunosensor for the early monitoring and risk assessment of chemotherapy-induced cardiotoxicity based on CRP-specific immunorecognition was successfully constructed. Under optimal experimental conditions, the immunosensor exhibited excellent linearity in the range of 3.9 × 10⁻¹ to 4 × 10² ng mL⁻¹ with a detection limit as low as 3.16 × 10⁻¹ ng mL⁻¹, making it more sensitive than ELISA. The immunosensor also demonstrated excellent selectivity, reproducibility, and stability in detecting CRP in a chemotherapy-induced cardiotoxicity model, with recovery rates ranging from 97.14 % to 107.65 %. This study presents an efficient, accurate, and practical approach for the early detection and risk assessment of chemotherapy-induced cardiotoxicity, thereby supporting a balance between therapeutic efficacy and cardiac safety.

癌症化疗虽然提高了患者的生存，但受到心脏毒性的限制。早期监测和风险评估至关重要。c反应蛋白（CRP）可以作为预测化疗期间心脏毒性的早期生物标志物，为开发新的心脏保护策略提供了重要机会。本研究将抗crp抗体负载在壳聚糖（CS）和功能化炭黑（f-CB）修饰的电极上。由于f-CB优良的导电性和CS的高通透性和强粘附性，我们成功构建了一种基于crp特异性免疫识别的化疗诱导心脏毒性早期监测和风险评估的免疫传感器。在最佳实验条件下，该免疫传感器在3.9 × 10−1 ~ 4 × 102 ng mL−1范围内具有良好的线性关系，检出限低至3.16 × 10−1 ng mL−1，灵敏度高于ELISA。该免疫传感器在化疗诱导的心脏毒性模型中检测CRP也表现出优异的选择性、重复性和稳定性，回收率为97.14%至107.65%。本研究为化疗引起的心脏毒性的早期检测和风险评估提供了一种高效、准确和实用的方法，从而支持治疗疗效和心脏安全性之间的平衡。

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引用次数: 0

Inhibition of microbiologically influenced corrosion in 90/10 CuNi alloy by a novel antibacterial peptide 新型抗菌肽对90/10 CuNi合金微生物腐蚀的抑制作用

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-16 DOI: 10.1016/j.bioelechem.2025.109114

Nianting Xue, Hao Sun, Shihang Lu, Yizhen Yu, Wenyan Ma, Guangzhou Liu, Wenwen Dou

Microbiologically influenced corrosion (MIC) poses a serious threat to the structural integrity of CuNi alloys under marine service conditions. This study proposed a novel antibacterial peptide (Tcs) combined with Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) to inhibit MIC in 90/10 CuNi alloy induced by Desulfovibrio ferrophilus IS5. Bacterial counts, environmental parameters analysis, surface morphological analyses, weight loss measurements, and electrochemical tests were conducted, through which the antibacterial and corrosion inhibition effects were demonstrated. The results showed that 180 ppb Peptide Tcs (180 Tcs) alone exerted almost no inhibitory effect on D. ferrophilus IS5, whereas a significant concentration-dependent enhancement was observed when Peptide Tcs was combined with 20 ppm ADBAC (20 ADBAC). Specifically, the 20 ppm ADBAC +180 ppb Peptide Tcs (20 ADBAC-180 Tcs) combination further reduced sessile bacterial counts by one order of magnitude compared to 20 ADBAC alone, while the weight loss rate and maximum pitting depth decreased by approximately 86 % and 61 %, respectively. These findings provide new insights into optimizing traditional biocide systems with small antimicrobial peptides and developing green, efficient strategies for controlling marine MIC.

在海洋环境下，微生物影响腐蚀（MIC）对CuNi合金的结构完整性构成严重威胁。本研究提出了一种新型抗菌肽（Tcs）与烷基二甲基苄基氯化铵（ADBAC）联合抑制嗜铁Desulfovibrio ferrophilus IS5诱导的90/10 CuNi合金MIC。通过细菌计数、环境参数分析、表面形态分析、失重测量和电化学测试，证明了抗菌和缓蚀效果。结果表明，180 ppb Peptide Tcs （180 Tcs）单独对嗜铁杆菌IS5几乎没有抑制作用，而与20 ppm ADBAC （20 ADBAC）联合使用时，对嗜铁杆菌IS5的抑制作用显著增强。具体来说，与单独使用20 ADBAC相比，20 ppm ADBAC +180 ppb Peptide Tcs （20 ADBAC-180 Tcs）组合进一步减少了一个数量级的无根细菌计数，而失重率和最大点蚀深度分别下降了约86%和61%。这些发现为优化使用小抗菌肽的传统杀菌剂系统和开发绿色、有效的海洋MIC控制策略提供了新的见解。

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引用次数: 0

Spectroelectrochemical studies of TDMQ20: A potential drug against Alzheimer's disease - part 2 - Cu-complexes 抗阿尔茨海默病潜在药物TDMQ20的光谱电化学研究——第二部分- cu络合物。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-16 DOI: 10.1016/j.bioelechem.2025.109115

Martin Perez-Estebanez , Fabiola Olmo-Alonso , Natalia Baran , Martin Jönsson-Niedziółka , Magdalena Z. Wiloch

Alzheimer's Disease (AD) has been associated with the overproduction reactive radicals through the catalytic effect of β-amyloid peptide Cu(II) complexes. In this article, we performed electrochemical characterisation of copper complexed with TDMQ20, which has been suggested as a potential drug against AD, using electrochemistry and spectroelectrochemistry (SEC). Based on voltammetry, we showed that oxidation as well as reduction processes of Cu(II)-TDMQ20 are pH-sensitive. Simulations of the CVs allowed us to estimate the kinetic parameters of the initial quasi-reversible oxidation of Cu(II) and the subsequent internal electron transfer to the Cu(III) ion from the TDMQ20 ligand (k₀ ≈ 18 s⁻¹). The electrochemical mechanism of oxidation/reduction of Cu(II)-TDM20 complex was also studied using UV-VIS absorption spectroelectrochemistry (UV-VIS SEC). UV-VIS SEC measurements showed that after oxidation of the ligand through the reduction of the Cu(III), the Cu remains complexed with the TDMQ20, but the electrochemical properties of the complex are changed. Our findings contribute to a broader understanding of the redox activity of the Cu(II)-TDMQ20 complex at physiological pH, which is a crucial aspect in evaluating the potential clinical utility of this compound.

通过β-淀粉样肽Cu（II）复合物的催化作用，阿尔茨海默病（AD）与反应性自由基的过量产生有关。在本文中，我们利用电化学和光谱电化学（SEC）对铜与TDMQ20络合进行了电化学表征，TDMQ20被认为是一种潜在的抗AD药物。基于伏安法，我们发现Cu(II)-TDMQ20的氧化和还原过程对ph敏感。模拟的cv使我们能够估计Cu（II）的初始准可逆氧化和随后从TDMQ20配体（k0≈18 s-1）向Cu（III）离子的内部电子转移的动力学参数。采用紫外-可见吸收光谱电化学（UV-VIS SEC）研究了Cu(II)-TDM20配合物氧化还原的电化学机理。UV-VIS SEC测试表明，通过还原Cu（III）氧化配体后，Cu仍然与TDMQ20络合，但络合物的电化学性能发生了变化。我们的发现有助于更广泛地了解Cu(II)-TDMQ20复合物在生理pH下的氧化还原活性，这是评估该化合物潜在临床应用的关键方面。

{"title":"Spectroelectrochemical studies of TDMQ20: A potential drug against Alzheimer's disease - part 2 - Cu-complexes","authors":"Martin Perez-Estebanez , Fabiola Olmo-Alonso , Natalia Baran , Martin Jönsson-Niedziółka , Magdalena Z. Wiloch","doi":"10.1016/j.bioelechem.2025.109115","DOIUrl":"10.1016/j.bioelechem.2025.109115","url":null,"abstract":"<div><div>Alzheimer's Disease (AD) has been associated with the overproduction reactive radicals through the catalytic effect of β-amyloid peptide Cu(II) complexes. In this article, we performed electrochemical characterisation of copper complexed with TDMQ20, which has been suggested as a potential drug against AD, using electrochemistry and spectroelectrochemistry (SEC). Based on voltammetry, we showed that oxidation as well as reduction processes of Cu(II)-TDMQ20 are pH-sensitive. Simulations of the CVs allowed us to estimate the kinetic parameters of the initial quasi-reversible oxidation of Cu(II) and the subsequent internal electron transfer to the Cu(III) ion from the TDMQ20 ligand (k<sub>0</sub> ≈ 18 s<sup>−1</sup>). The electrochemical mechanism of oxidation/reduction of Cu(II)-TDM20 complex was also studied using UV-VIS absorption spectroelectrochemistry (UV-VIS SEC). UV-VIS SEC measurements showed that after oxidation of the ligand through the reduction of the Cu(III), the Cu remains complexed with the TDMQ20, but the electrochemical properties of the complex are changed. Our findings contribute to a broader understanding of the redox activity of the Cu(II)-TDMQ20 complex at physiological pH, which is a crucial aspect in evaluating the potential clinical utility of this compound.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109115"},"PeriodicalIF":4.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a highly efficient DNA-based biosensor using a functionalized MoS₂/polypyrrole nanocomposite for mitomycin C determination 基于功能化MoS 2 /聚吡咯纳米复合材料的高效dna生物传感器的开发用于丝裂霉素C的测定。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-13 DOI: 10.1016/j.bioelechem.2025.109111

Afsaneh Mousa Pour , Zahra Garkani-Nejad , Hadi Mahmoudi-Moghaddam

In this study, plate-like Eu³⁺-doped MoS₂ nanoparticles (PL-Eu³⁺-doped MoS₂ NPs) were successfully synthesized via a simple hydrothermal method and characterized using energy-dispersive X-ray spectroscopy (EDX), field-emission scanning electron microscopy (FESEM), and X-ray diffraction (XRD). Subsequently, a novel electrochemical DNA biosensor was developed by modifying pencil graphite electrodes (PGEs) with the synthesized nanocomposite and polypyrrole (PPy) for the sensitive detection of mitomycin C (MC). The interaction between double-stranded DNA (ds-DNA) and MC was thoroughly investigated using differential pulse voltammetry (DPV), spectrophotometry, and molecular docking methods. The decrease in the oxidation peak currents of adenine base in an acetate buffer solution (pH 4.80) was employed as a reliable indicator of the interaction event. The constructed biosensor (ds-DNA/PL-Eu³⁺-doped MoS₂/PGE) exhibited a linear response toward MC concentrations ranging from 1 to 75 μg/mL, achieving a low detection limit of 0.8 μg/mL. The sensor's performance was further validated in real sample analyses, demonstrating excellent recovery values between 97 % and 104 %, thereby confirming its potential for practical applications in biomedical and environmental monitoring. UV/Vis spectroscopic data combined with molecular docking analyses indicated substantial interaction between mitomycin and DNA, with evidence supporting groove binding as the predominant binding mode.

在本研究中，通过简单的水热法成功合成了片状Eu3+掺杂MoS₂纳米颗粒（PL-Eu3+掺杂MoS₂NPs），并利用能量色散x射线能谱（EDX）、场发射扫描电镜（FESEM）和x射线衍射（XRD）对其进行了表征。随后，利用合成的纳米复合材料和聚吡咯（PPy）修饰铅笔石墨电极（PGEs），开发了一种新型电化学DNA生物传感器，用于丝裂霉素C （MC）的灵敏检测。利用差分脉冲伏安法（DPV）、分光光度法和分子对接方法对双链DNA （ds-DNA）和MC之间的相互作用进行了深入的研究。在pH为4.80的醋酸缓冲溶液中，腺嘌呤碱氧化峰电流的下降被用作相互作用事件的可靠指标。构建的生物传感器（ds-DNA/PL-Eu3+掺杂MoS₂/PGE）对MC浓度在1 ~ 75 μg/mL范围内呈线性响应，检测限低至0.8 μg/mL。在实际样品分析中进一步验证了传感器的性能，显示出97% ~ 104%的优异回收率，从而证实了其在生物医学和环境监测方面的实际应用潜力。紫外/可见光谱数据结合分子对接分析表明丝裂霉素与DNA之间存在大量相互作用，有证据支持凹槽结合是主要的结合方式。

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引用次数: 0