Bioelectrochemistry最新文献_第10页

Influence of initial cell counts on the microbiologically influenced corrosion of L245N steel in shale gas environments 初始细胞数对页岩气环境中L245N钢微生物腐蚀的影响

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-11-08 DOI: 10.1016/j.bioelechem.2025.109157

Yanran Wang , Hongfa Huang , Yongfan Tang , Shaomu Wen , Xulin Hou

Microbiologically influenced corrosion (MIC) caused by sulfate-reducing bacteria (SRB) poses a major threat to pipeline integrity in shale gas operations. Current industry standards set a control threshold of 25 cells/mL for SRB, but its scientific validity remains unclear. This study investigates the corrosion behavior of L245N steel under varying initial SRB concentrations (10⁴ to 25 cells/mL) with 50 ppm THPS biocide presence. Results reveal a distinct threshold effect between 10³ and 10² cells/mL, where corrosion severity, biofilm thickness, and sessile cell count drop sharply. Further reductions below 10² cells/mL produce negligible corrosion impact. However, there is no substantial difference between 100, 50, and 25 cells/mL, indicating a plateau effect. Sessile cell counts, biofilm morphology, electrochemical parameters, and corrosion rates all support this finding. Additionally, mixed microbial consortia enhanced SRB survival under biocidal conditions. These results suggest that the current standard may not be sufficient for MIC prevention and highlight the need for revised standards based on corrosion behavior rather than planktonic cell counts alone.

在页岩气作业中，由硫酸盐还原菌（SRB）引起的微生物影响腐蚀（MIC）对管道完整性构成了重大威胁。目前的行业标准为SRB设定了25个细胞/mL的控制阈值，但其科学有效性尚不清楚。本研究研究了L245N钢在不同初始SRB浓度（104 ~ 25 cells/mL）和50 ppm THPS杀菌剂存在下的腐蚀行为。结果显示，在103和102细胞/mL之间存在明显的阈值效应，腐蚀严重程度、生物膜厚度和无根细胞计数急剧下降。进一步降低到102格/毫升以下，腐蚀影响可以忽略不计。然而，在100、50和25个细胞/mL之间没有实质性差异，表明存在平台效应。无孔细胞计数、生物膜形态、电化学参数和腐蚀速率都支持这一发现。此外，混合菌群可提高SRB在杀生物剂条件下的存活率。这些结果表明，目前的标准可能不足以预防MIC，并强调需要根据腐蚀行为修订标准，而不仅仅是浮游细胞计数。

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引用次数: 0

Investigations of potential therapeutic targets from high-throughput, label-free, quantitative proteomic studies in MDA-MB-468 cells treated with metformin and electrical pulses 二甲双胍和电脉冲处理MDA-MB-468细胞的高通量、无标记、定量蛋白质组学研究的潜在治疗靶点研究。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-10-24 DOI: 10.1016/j.bioelechem.2025.109153

Praveen Sahu , Ignacio G. Camarillo , Raji Sundararajan

High-throughput, label-free quantitative proteomic analyses, along with cell viability and reactive oxygen species (ROS) studies, were performed on MDA-MB-468 human triple-negative breast cancer (TNBC) cells, to gain mechanistic insights into therapeutic actions. TNBC was chosen as it is the most lethal subtype of breast cancer, highly aggressive, prone to recurrence and metastasis, with the highest prevalence in black women. The absence of specific biomarkers limits targeted therapies. This unmet need was studied using proteomics, after treating the cells with electrical pulses (EP) combined with metformin (met). With the EP + met treatment (1000 V/cm, 1 mM), cell viability dropped to 25.6 % after 24 h and ROS increased to 179 %, compared to control at 100 %. Proteomics revealed 125 upregulated and 37 downregulated proteins in EP + met, compared to met alone, involving enzymes, proliferation markers, and kinases. Key gene changes included upregulation of ALAD, MKI67, and LRCH4, and downregulation of EIF1AX, NSUN5, and GNS. LRCH4 and GNS are suggested to be potential novel therapeutic targets, as LRCH4 upregulation links to inhibition of the mTOR/PI3K/Akt pathway, reducing proliferation, while GNS downregulation suppresses tumor growth and metastasis. Overall, proteomics-based preliminary findings suggest that EP + met modulate TNBC pathways, identifying potential biomarkers and providing a foundation for future validation

在MDA-MB-468人三阴性乳腺癌（TNBC）细胞上进行了高通量、无标记的定量蛋白质组学分析，以及细胞活力和活性氧（ROS）研究，以获得治疗作用的机制见解。选择TNBC是因为它是乳腺癌中最致命的亚型，具有高度侵袭性，容易复发和转移，在黑人妇女中发病率最高。特异性生物标志物的缺乏限制了靶向治疗。在电脉冲（EP）联合二甲双胍（met）处理细胞后，使用蛋白质组学研究了这种未满足的需求。EP + met （1000 V/cm, 1 mM）处理24 h后，细胞活力下降到25.6%，ROS增加到179%，而对照组为100%。蛋白质组学显示，与单独使用EP + met相比，EP + met中有125个蛋白上调，37个蛋白下调，涉及酶、增殖标志物和激酶。关键基因变化包括ALAD、MKI67和LRCH4上调，EIF1AX、NSUN5和GNS下调。LRCH4和GNS被认为是潜在的新治疗靶点，因为LRCH4上调与抑制mTOR/PI3K/Akt通路有关，减少增殖，而GNS下调抑制肿瘤生长和转移。总的来说，基于蛋白质组学的初步研究结果表明，EP + met调节TNBC通路，识别潜在的生物标志物，并为未来的验证提供基础。

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引用次数: 0

Bioelectricity generation via biodegradation of pharmaceutical wastewater using MnCo2O4-CV coated electrodes in dual-chamber microbial fuel cells 利用MnCo2O4-CV包覆电极在双室微生物燃料电池中生物降解制药废水产生生物电。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-11-11 DOI: 10.1016/j.bioelechem.2025.109165

A.D. Ettiyan , Tamilarasan K , Siva P.R. Muppala

This study presents an innovative and environmentally sustainable approach for treating pharmaceutical wastewater (PWW) using a dual-chamber microbial fuel cell (DMFC) that simultaneously generates bioelectricity. The DMFC system incorporates manganese cobalt oxide-coated carbon veil (MnCo₂O₄-CV) electrodes to optimize organic pollutant removal and enhance power production from PWW. The novel MnCo₂O₄-CV electrode coating represents a significant advancement, offering superior chemical stability, electrical conductivity, durability, large surface area, and enhanced absorption capacity. Following a systematic acclimatization, various organic loadings were investigated to identify optimal operating conditions. Results demonstrated peak performance at an organic loading of 2.0 g COD/L. Under these conditions, the system exhibited remarkable removal efficiencies for total chemical oxygen demand (TCOD), soluble chemical oxygen demand (SCOD), and total suspended solids (TSS), while generating electrical output. Performance evaluation encompassed maximum voltage, current density, power density, coulombic efficiency, and pollutant removal metrics. Microbial community analysis via 16S rRNA gene sequencing revealed a diverse bacterial community in the anodic biofilm that contributed to improved system performance.

本研究提出了一种创新和环境可持续的方法，使用双室微生物燃料电池（DMFC）同时产生生物电来处理制药废水（PWW）。DMFC系统采用锰钴氧化物涂层碳膜（MnCo2O4-CV）电极，以优化有机污染物的去除并提高PWW的发电量。新型MnCo2O4-CV电极涂层代表了一项重大进步，具有卓越的化学稳定性、导电性、耐久性、大表面积和增强的吸收能力。在系统驯化后，研究了各种有机负荷，以确定最佳操作条件。结果表明，有机负荷为2.0 g COD/L时性能最佳。在这些条件下，该系统在产生电力输出的同时，对总化学需氧量（TCOD）、可溶性化学需氧量（SCOD）和总悬浮物（TSS）表现出了显著的去除效率。性能评估包括最大电压、电流密度、功率密度、库仑效率和污染物去除指标。通过16S rRNA基因测序对微生物群落进行分析，发现阳极生物膜中的细菌群落多样性有助于提高系统性能。

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引用次数: 0

Electrochemical sensor for urinary H2O2 detection to aid AKI diagnosis and treatment evaluation 电化学传感器尿液H2O2检测辅助AKI诊断和治疗评价。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-11-16 DOI: 10.1016/j.bioelechem.2025.109173

Cheng Huang , Haowen Liu , Bao Jiang , Guoli Li , Xinlu Qin , Yinan Hua , Yongming Deng , Yicheng Wang , Lin Zhou

Acute kidney injury (AKI), a critical clinical syndrome marked by high incidence and mortality, is currently diagnosed mainly by serum creatinine (SCr) and blood urea nitrogen (BUN), which have high miss rates. This study innovatively proposes using urinary hydrogen peroxide (H₂O₂) concentration changes, caused by renal oxidative stress in AKI, as a new indicator for AKI risk assessment and treatment monitoring. Results from in vitro and AKI animal models show this indicator can quickly monitor AKI onset and drug effects in mice via electrochemical sensing technology based on Bi₂S₃@Cu_0.1, offering a novel approach for AKI diagnosis and rehabilitation monitoring.

急性肾损伤（Acute kidney injury， AKI）是一种发病率高、死亡率高的临床重症综合征，目前主要通过血清肌酐（SCr）和血尿素氮（BUN）进行诊断，漏检率高。本研究创新性地提出将肾氧化应激引起的尿中过氧化氢（H2O2）浓度变化作为AKI风险评估和治疗监测的新指标。体外和AKI动物模型结果表明，该指标可以通过基于Bi2S3@Cu0.1的电化学传感技术快速监测小鼠AKI的发病和药物效应，为AKI的诊断和康复监测提供了一种新的方法。

引用次数: 0

Carboxyl-functionalized covalent organic framework with precisely matched pore size achieving effective loading of cytochrome C for electrochemical biosensors 具有精确匹配孔径的羧基功能化共价有机骨架实现了电化学生物传感器细胞色素C的有效负载。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-11-14 DOI: 10.1016/j.bioelechem.2025.109169

HaoXian He , JianBing Li , JianMing Liu, LongSheng Pei, LongFei Miao, YongHai Song, LiMin Liu, Li Wang

The immobilization of enzymes is crucial for enhancing their catalytic activity and stability. Covalent organic frameworks (COF), with abundant active sites and tunable pore structures, enable effective immobilization of enzymes. Here, we designed carboxyl-functionalized COF (COF-COOH) to immobilize Cytochrome C (Cyt C), aiming to regulate the perfect pairing of the COF pore (3.67 nm) and the Cyt C dimension (2.6 nm × 3.2 nm × 3.3 nm). Meanwhile, the large amount of -COOH can increase the electrostatic and hydrogen bonding forces between COF-COOH and Cyt C. Thus, the Cyt C was efficiently loaded into COF-COOH through the post-modification method (loading efficiency = 62.37 %). The catalytic activity (k_cat/K_m) of Cyt C@COF-COOH toward H₂O₂ was significantly enhanced to 309.96 s⁻¹ M⁻¹ as compared to free Cyt C of 105.55 s⁻¹ M⁻¹. The catalytic activity of Cyt C@COF-COOH toward H₂O₂ still exceeds 80 % in some harsh environments (acetonitrile, dimethyl sulfoxide, tetrahydrofuran and 60 °C). The detection range of electrochemical H₂O₂ biosensor based on Cyt C@COF-COOH is as wide as 2.0–80 μM, and the sensitivity is as high as 0.373 μA μM⁻¹ cm⁻².

酶的固定化是提高酶的催化活性和稳定性的关键。共价有机框架（COF）具有丰富的活性位点和可调节的孔结构，可以有效地固定酶。我们设计了羧基功能化COF （COF- cooh）来固定细胞色素C (Cyt C)，旨在调节COF孔（3.67 nm）和Cyt C尺寸（2.6 nm × 3.2 nm × 3.3 nm）的完美配对。同时，大量的-COOH增加了COF-COOH与Cyt C之间的静电力和氢键力，从而通过后修饰的方法将Cyt C高效加载到COF-COOH中（加载效率为62.37%）。与游离Cyt C的105.55 s-1 M-1相比，Cyt C@COF-COOH对H2O2的催化活性（kcat/Km）显著提高至309.96 s-1 M-1。在一些恶劣环境（乙腈、二甲亚砜、四氢呋喃、60℃）下，Cyt C@COF-COOH对H2O2的催化活性仍然超过80%。基于Cyt C@COF-COOH的电化学H2O2生物传感器的检测范围为2.0 ~ 80 μM，灵敏度高达0.373 μA μM-1 cm-2。

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引用次数: 0

The assessment of interaction kinetics between specific monoclonal antibody and immobilized SARS-CoV-2 nucleoprotein 特异性单克隆抗体与固定化SARS-CoV-2核蛋白相互作用动力学评价

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-08-25 DOI: 10.1016/j.bioelechem.2025.109090

Yana Karnitskaya , Maryia Drobysh , Almira Ramanaviciene , Agne Rimkute , Indre Kucinskaite-Kodze , Greta Zvirzdine , Rimantas Slibinskas , Oksana Pogorielova , Viktoriia Korniienko , Arunas Ramanavicius

The emergence of SARS-CoV-2 has posed significant global health challenges. The nucleocapsid protein (N-protein) is a structural part of the SARS-CoV-2 virus and an important immunogenic target of specific antibodies, which are developed in the organism during the infection by this virus. Artificially designed specific (monoclonal and polyclonal) antibodies are also used for therapeutic and bioanalytical purposes, therefore, the assessment and characterization of newly designed antibodies is an important analytical issue. This study reports an electrochemical biosensing system for the assessment of the interaction between newly designed specific antibody and SARS-CoV-2 recombinant N-protein, against which the antibody was designed, and which was used as a target in biological recognition part of the biosensing system. The biosensing system was applied for the determination of the interaction kinetics between immobilized N-protein and a newly derived monoclonal antibody (mAb) 16D9 (mAb-16D9). Cyclic voltammetry was employed to evaluate the kinetics of the interaction between the recombinant N-protein and mAb-16D9. The binding constant (Kc) was determined to be 50.99 μg/mL, demonstrating a strong affinity, while the limit of detection was 4.3 × 10⁻⁴ μg/mL, highlighting sufficient affinity of mAb-16D9 towards N-protein, which determined good sensitivity of the developed biosensors. These findings highlight the potential application of the here-reported electrochemical biosensor for rapid testing of antigen-antibody interaction kinetics and the characterization of newly designed antibody.

SARS-CoV-2的出现构成了重大的全球卫生挑战。核衣壳蛋白（n蛋白）是SARS-CoV-2病毒的结构组成部分，是机体在感染该病毒过程中产生的特异性抗体的重要免疫原性靶点。人工设计的特异性（单克隆和多克隆）抗体也用于治疗和生物分析目的，因此，新设计抗体的评估和表征是一个重要的分析问题。本研究报道了一种电化学生物传感系统，用于评估新设计的特异性抗体与SARS-CoV-2重组n蛋白之间的相互作用，并将其作为生物传感系统生物识别部分的靶点。应用该生物传感系统测定了固定化n蛋白与新获得的单克隆抗体（mAb） 16D9 （mAb-16D9）的相互作用动力学。采用循环伏安法评价重组n蛋白与mAb-16D9相互作用的动力学。结合常数（Kc）为50.99 μg/mL，具有较强的亲和力，检出限为4.3 × 10−4 μg/mL，表明单克隆抗体- 16d9对n蛋白具有较强的亲和力，具有较好的灵敏度。这些发现突出了本文报道的电化学生物传感器在快速检测抗原-抗体相互作用动力学和新设计抗体表征方面的潜在应用。

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引用次数: 0

Biocorrosion studies on borated and non-borated 304 L stainless steel using Bacillus subtilis SNF-1, a bacterial isolate from SNF pool 利用枯草芽孢杆菌SNF-1对含硼和非含硼304 L不锈钢的生物腐蚀研究

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-09-09 DOI: 10.1016/j.bioelechem.2025.109106

Namrata Upadhyay , Sudhir K. Shukla , N. Malathy , Y.V. Nancharaiah , A. Ravi Shankar , S. Ningshen

This study investigates microbiologically-influenced corrosion (MIC) aspects of borated and non-borated 304 L- stainless-steel using Bacillus subtilis SNF-1, which was isolated from the spent nuclear fuel pool (SNF). Over 28 days, electrochemical analyses revealed distinct corrosion behaviours: borated 304 L SS exhibited a more pronounced decrease in open circuit potential (from 0.03 to −0.35 V vs. Ag/AgCl) as compared to non-borated 304 L SS (from 0.05 to −0.10 V vs. Ag/AgCl) indicating higher susceptibility to MIC. Potentiodynamic polarization studies revealed an increase in passive current density (from 1.5 to 2.4 μA.cm⁻² for non-borated 304 L SS and from 2.4 to 3.4 μA.cm⁻² for borated 304 L SS), along with a lower pitting potential indicating the role of B. subtilis SNF-1 in MIC. Electrochemical impedance spectroscopy confirmed accelerated degradation, with polarization resistance (R_p) dropping by 69 % in borated 304 L SS and 86 % in non-borated 304 L SS. Despite higher absolute corrosion rates in borated 304 L SS, non-borated 304 L SS experienced a greater relative increase in corrosion (3.8-fold vs. 2.3-fold) due to denser biofilm coverage (95 % vs. 74 %). Surface analysis identified localized pitting beneath biofilms, exacerbated by boride-induced micro-galvanic effects. These findings underscore the dual role of alloy microstructure and biofilm dynamics in MIC severity.

本研究利用从乏燃料池（SNF）中分离出来的枯草芽孢杆菌SNF-1研究了硼化和非硼化304 L不锈钢的微生物影响腐蚀（MIC）问题。在28天的时间里，电化学分析显示了不同的腐蚀行为：与未含硼的304 L SS（从0.05到- 0.10 V）相比，含硼的304 L SS的开路电位（从0.03到- 0.35 V vs Ag/AgCl）下降更为明显，表明对MIC的敏感性更高。动电位极化研究表明，无源电流密度增加（未加硼的304 L SS从1.5 μA.cm−2增加到2.4 μA.cm−2，加硼的304 L SS从2.4 μA.cm−2增加到3.4 μA.cm−2），同时点蚀电位降低，表明枯草芽孢杆菌SNF-1在MIC中的作用。电化学阻抗谱证实了加速降解，含硼304 L SS的极化电阻（Rp）下降了69%，未含硼304 L SS的极化电阻（Rp）下降了86%。尽管含硼304 L SS的绝对腐蚀速率更高，但由于生物膜覆盖率更高（95%对74%），未含硼304 L SS的腐蚀相对增加更大（3.8倍对2.3倍）。表面分析确定了生物膜下的局部点蚀，由硼化物引起的微电效应加剧。这些发现强调了合金微观结构和生物膜动力学在MIC严重程度中的双重作用。

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引用次数: 0

Electron transfer performance and mechanism in twin microbial fuel cell powered electro-Fenton system with waste activated sludge as substrate 以废活性污泥为底物的双微生物燃料电池电fenton系统的电子传递性能及机理。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-10-25 DOI: 10.1016/j.bioelechem.2025.109154

Jiaqi Lv , Qingliang Zhao , Junqiu Jiang , Jing Ding , Liangliang Wei , Jiawen Liang

The twin microbial fuel cell powered electro-Fenton system (twin-MFCⓅEFs), combining active oxygen component and microbial metabolism, was constructed to improve the treatment process of waste activated sludge (WAS). Nevertheless, the performance and mechanism of electron transfer underlying this enhancement remain poorly understood. This study investigated the performance and mechanism of electron generation and utilization in twin-MFCⓅEFs with WAS as substrate. The higher electron generation and recovery efficiency (8.25 % of coulombic efficiency) was attributed to the higher content of amino acids (such as tryptophan), humic substances and their aromatic groups and unsaturated conjugated double bonds in the soluble organic matter, which facilitated biodegradation and electron transfer. The higher electron utilization performance (52.76 % of faraday efficiency) relied on the superior electron supply system that exhibited greater free radical oxidation. Metagenomic analysis indicated that an increased secretory capacity of glycosyltransferases (including glucosyltransferases and β-glucosidases) and a reduced activity of acetate kinase and methyl-coenzyme M reductase alpha subunit in cellular metabolic processes favored signaling and electricity production. The study focused on electron flow in twin-MFCⓅEFs and offered a promising strategy for improving the sludge treatment process.

为改进废活性污泥（was）的处理工艺，构建了将活性氧组分与微生物代谢相结合的双微生物燃料电池供电电fenton系统（twin- mfcⓅEFs）。然而，这种增强背后的电子转移的性能和机制仍然知之甚少。本文研究了以WAS为底物的双mfcⓅ电子产生和利用的性能和机制。可溶有机物中氨基酸（如色氨酸）、腐殖质及其芳香基团和不饱和共轭双键的含量较高，有利于生物降解和电子传递，电子生成和回收效率较高（库仑效率为8.25%）。较高的电子利用性能（52.76%的法拉第效率）依赖于表现出更强自由基氧化的优越电子供应系统。宏基因组分析表明，细胞代谢过程中糖基转移酶（包括葡萄糖基转移酶和β-葡萄糖苷酶）分泌能力的增加和乙酸激酶和甲基辅酶M还原酶α亚基活性的降低有利于信号传导和电力产生。研究了双mfcⓅEFs中的电子流，为改善污泥处理工艺提供了一种有希望的策略。

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引用次数: 0

Electroactive TiO₂–Cu nanotube platform with synergistic charge modulation and Cu2+ release for marine antifouling 具有协同电荷调制和Cu2+释放的电活性tio2 -Cu纳米管平台用于船舶防污。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-10-11 DOI: 10.1016/j.bioelechem.2025.109135

Yuqiao Dong , Yuxuan Xu , Jinke Yin , Qianwen Jin , Guangzhou Liu

Biological damage remains a critical limiting factor that hinders the application of highly biocompatible titanium alloys in marine engineering. Exploiting the delicate electrostatic interactions at the interface between organisms and nanomaterials is of vital importance. In this study, an external direct current was applied to copper-coated capacitive TiO₂ nanotubes (TNT-Cu) to evaluate the antifouling effect on sessile biofilms and planktonic bacteria, and to elucidate the underlying mechanisms. The heterostructure exhibited high specific capacitance and superior antifouling performance. Upon electrochemical charge-discharge, TNT-Cu achieved 98.3 ± 0.9 % adhesion inhibition and 96.0 ± 2.0 % algicidal activity against Phaeodactylum tricornutum. The charged TNT-Cu achieved synergistic antifouling through surface charge–induced electrostatic sterilization and controlled Cu²⁺ release. Physiologically, the electrical interaction combined with Cu²⁺ significantly disrupted algal electron transport, induced reactive oxygen species (ROS) accumulation, and caused membrane rupture. This work provides a promising, durable, and eco-friendly antifouling strategy for marine applications of titanium-based materials.

生物损伤是制约高生物相容性钛合金在海洋工程中应用的重要因素。利用生物与纳米材料界面上微妙的静电相互作用是至关重要的。本研究通过对铜包覆的电容性纳米管（TNT-Cu）施加外部直流电，考察其对固定式生物膜和浮游细菌的防污作用，并探讨其机制。该异质结构具有较高的比电容和优异的防污性能。电化学充放电后，TNT-Cu对三角褐指藻的粘附抑制率为98.3%±0.9%，杀藻活性为96.0%±2.0%。带电荷的TNT-Cu通过表面电荷诱导的静电灭菌和控制Cu2+释放来实现协同防污。生理上，电相互作用与Cu2+的结合显著破坏了藻类的电子传递，诱导活性氧（ROS）积累，并导致膜破裂。这项工作为钛基材料的海洋应用提供了一种有前途的、耐用的、环保的防污策略。

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引用次数: 0

Ultrasensitive electrochemiluminescence immunosensor based on gold-functionalised NiFe layered double hydroxide and electro-negative mesoporous silica for prostate-specific antigen detection 基于金功能化NiFe层状双氢氧化物和电负介孔二氧化硅的超灵敏电化学发光免疫传感器用于前列腺特异性抗原检测。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-10-18 DOI: 10.1016/j.bioelechem.2025.109145

Dongcheng Yang , Huan Wang , Caiyu Wang , Xinyu Liu , Lihua Hu , Hongmin Ma , Dan Wu , Xiang Ren , Yuyang Li , Qin Wei

Electrochemiluminescence (ECL) technology has become an essential analytical methodology in biomolecular detection, attaining both profound research advancements and extensive practical applications in this domain. Herein, we used carboxylated mesoporous silica (MSN-COOH) as a container to encapsulate Tris(2,2-bipyridyl)ruthenium(II)²⁺ (Ru(bpy)₃²⁺)—a luminophore—in its pores to achieve enrichment (RuMSN). The reactive intermediates Ru(bpy)₃³⁺ and TPrA^•+ are attracted by the electro-negative carboxyl group on the MSN-COOH surface, substantially reducing the reaction distance and improving the ECL response efficiency. The flower-like NiFe layered double hydroxide (NiFe-LDH) nanostructure possesses significantly high specific surface area and good electrocatalytic performance, while gold nanoparticles also demonstrate excellent electrical conductivity and biocompatibility. To enhance sensor sensitivity, gold nanoparticles were loaded onto the NiFe-LDH surface forming the Au@NiFe-LDH composite, which improves performance by enhancing conductive properties and increasing antigen-antibody binding sites. This study established a sandwich-configuration electrochemiluminescence detection platform capable of highly sensitive PSA analysis.The biosensor demonstrated excellent stability, specificity and selectivity, with a linear detection range of 0.1 pg/mL-50 ng/mL and a detection limit as low as 63 fg/mL (S/N = 3). This confirms the clinical application value of the detection system in the early screening of prostate cancer.

电化学发光（ECL）技术已成为生物分子检测中必不可少的分析方法，在该领域取得了深刻的研究进展和广泛的实际应用。本文采用羧化介孔二氧化硅（MSN-COOH）作为容器，将Tris（2,2-联吡啶基）钌（II）2+ (Ru(bpy)32+)-发光团包封在其孔隙中，实现富集（RuMSN）。反应中间体Ru(bpy)33+和TPrA•+被MSN-COOH表面的电负羧基吸引，大大缩短了反应距离，提高了ECL响应效率。这种花状NiFe层状双氢氧化物（NiFe- ldh）纳米结构具有显著的高比表面积和良好的电催化性能，而金纳米颗粒也具有优异的导电性和生物相容性。为了提高传感器的灵敏度，将金纳米颗粒加载到nfe - ldh表面形成Au@NiFe-LDH复合材料，通过增强导电性能和增加抗原-抗体结合位点来提高性能。本研究建立了一个具有高灵敏度PSA分析能力的三明治结构电化学发光检测平台。该传感器具有良好的稳定性、特异性和选择性，线性检测范围为0.1 pg/mL ~ 50 ng/mL，检出限低至63 fg/mL （S/N = 3）。这证实了该检测系统在前列腺癌早期筛查中的临床应用价值。

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引用次数: 0