Pub Date : 2025-11-03DOI: 10.1016/j.bioelechem.2025.109161
R. Divya Mohan , Shashanka Rajendrachari , Habdias de Araujo Silva Neto , A. Santhy , R. Rejithamol
The presence of elevated glutamate levels is linked to a variety of neurological disorders, creating an urgent demand for advancements in glutamate detection technologies. This review underscores the major advancements and breakthroughs that have influenced the development of L-glutamate biosensing technologies, showcasing the significant progress made over time. Electrochemical glutamate sensors are widely used for real-time in vivo monitoring of glutamate with high temporal resolution. Electrochemical sensors can detect rapid glutamate changes on the order of seconds or faster, providing sub-second temporal resolution that captures transient neurotransmitter release events. Enzyme-based microsensors have demonstrated detection limits in the low micromolar or hundreds or tens-of-nanomolar range, suitable for physiological extracellular glutamate levels. This review focuses on the advances in enzymatic electrochemical sensors for the neurotransmitter L-Glutamate in brain fluids.
{"title":"Exploration and advances in enzymatic electrochemical biosensors for in vivo detection of brain glutamate","authors":"R. Divya Mohan , Shashanka Rajendrachari , Habdias de Araujo Silva Neto , A. Santhy , R. Rejithamol","doi":"10.1016/j.bioelechem.2025.109161","DOIUrl":"10.1016/j.bioelechem.2025.109161","url":null,"abstract":"<div><div>The presence of elevated glutamate levels is linked to a variety of neurological disorders, creating an urgent demand for advancements in glutamate detection technologies. This review underscores the major advancements and breakthroughs that have influenced the development of L-glutamate biosensing technologies, showcasing the significant progress made over time. Electrochemical glutamate sensors are widely used for real-time in vivo monitoring of glutamate with high temporal resolution. Electrochemical sensors can detect rapid glutamate changes on the order of seconds or faster, providing sub-second temporal resolution that captures transient neurotransmitter release events. Enzyme-based microsensors have demonstrated detection limits in the low micromolar or hundreds or tens-of-nanomolar range, suitable for physiological extracellular glutamate levels. This review focuses on the advances in enzymatic electrochemical sensors for the neurotransmitter L-Glutamate in brain fluids.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109161"},"PeriodicalIF":4.5,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145457042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-03DOI: 10.1016/j.bioelechem.2025.109160
Pengwei Jing , Xinhua Yang , Haokang Zhang , Guishi Li , Zengliang Wang
Osteosarcoma, a prevalent age-related condition, often goes undiagnosed due to expensive and invasive detection methods. This study presents a novel, cost-effective, non-invasive electrochemical sensor for Osteosarcoma detection, leveraging peptide probes to selectively recognize key biomarkers like iron ions and osteocalcin. Using a phospholipid monolayer and a conductive substrate, the sensor utilizes peptide probes containing a tripeptide iron-binding sequence and an osteocalcin sequence to detect ferroptosis-induced iron ions and elevated osteocalcin levels, both indicative of early-stage Osteosarcoma. Electrochemical modulation facilitates the covalent assembly of osteocalcin into nanoscale aggregates, significantly amplifying the sensor's signal. This design avoids the need for complex antibodies or nanomaterials, enhancing affordability and simplicity. By integrating everyday components like toothpaste to form a low baseline signal and utilizing saliva as the sample, the sensor offers high sensitivity and a low-cost alternative to traditional diagnostic methods. This innovative approach combines bioinspired materials and electrochemical techniques to provide a promising solution for early Osteosarcoma detection, addressing the pressing need for accessible diagnostics in aging populations.
{"title":"Bioinspired electrochemical supercapacitor: peptide-driven osteosarcoma detection via hydroxyapatite formation and ferroptosis modulation","authors":"Pengwei Jing , Xinhua Yang , Haokang Zhang , Guishi Li , Zengliang Wang","doi":"10.1016/j.bioelechem.2025.109160","DOIUrl":"10.1016/j.bioelechem.2025.109160","url":null,"abstract":"<div><div>Osteosarcoma, a prevalent age-related condition, often goes undiagnosed due to expensive and invasive detection methods. This study presents a novel, cost-effective, non-invasive electrochemical sensor for Osteosarcoma detection, leveraging peptide probes to selectively recognize key biomarkers like iron ions and osteocalcin. Using a phospholipid monolayer and a conductive substrate, the sensor utilizes peptide probes containing a tripeptide iron-binding sequence and an osteocalcin sequence to detect ferroptosis-induced iron ions and elevated osteocalcin levels, both indicative of early-stage Osteosarcoma. Electrochemical modulation facilitates the covalent assembly of osteocalcin into nanoscale aggregates, significantly amplifying the sensor's signal. This design avoids the need for complex antibodies or nanomaterials, enhancing affordability and simplicity. By integrating everyday components like toothpaste to form a low baseline signal and utilizing saliva as the sample, the sensor offers high sensitivity and a low-cost alternative to traditional diagnostic methods. This innovative approach combines bioinspired materials and electrochemical techniques to provide a promising solution for early Osteosarcoma detection, addressing the pressing need for accessible diagnostics in aging populations.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109160"},"PeriodicalIF":4.5,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145547454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-03DOI: 10.1016/j.bioelechem.2025.109162
Beihua Wen , Zhenyu Zhu , Junjie Zeng , Shiwei Wan , Chaoxin Zhang , Yingzhan Chen , Liwei Wang , Man Zhang , Kefu Yu
Recurring infestations of crown-of-thorns starfish (COTS) inflict severe harm on coral reef ecosystems. To prevent irreversible harm to coral ecosystems, early monitoring of COTS distribution before outbreaks is crucial for timely intervention and effective management. However, traditional monitoring methods are time-consuming, labor-intensive, and lack the sensitivity required for early warning and proactive control. In this study, a ratiometric electrochemical DNA biosensor was constructed for the detection of environmental DNA (eDNA) from COTS. This biosensor combines a dual-signal detection mechanism with internal calibration provided by a reference probe, along with a target recycling amplification strategy mediated by exonuclease III (Exo III). The biosensor exhibited outstanding analytical performance, with a low detection limit (LOD)of 93 fM and a linear response range spanning from 300 fM to 10 nM. Moreover, digital droplet PCR (ddPCR) verified the biosensor's accuracy and reliability (P > 0.05). The biosensor was successfully applied to detect COTS eDNA in environmental samples collected from the Paracel Islands. Therefore, the electrochemical biosensor is promising for detecting low-density COTS individuals or larvae before outbreaks, enabling early warning and facilitating timely ecological intervention.
{"title":"A ratiometric electrochemical DNA biosensor for the detection of crown-of-thorns starfish eDNA","authors":"Beihua Wen , Zhenyu Zhu , Junjie Zeng , Shiwei Wan , Chaoxin Zhang , Yingzhan Chen , Liwei Wang , Man Zhang , Kefu Yu","doi":"10.1016/j.bioelechem.2025.109162","DOIUrl":"10.1016/j.bioelechem.2025.109162","url":null,"abstract":"<div><div>Recurring infestations of crown-of-thorns starfish (COTS) inflict severe harm on coral reef ecosystems. To prevent irreversible harm to coral ecosystems, early monitoring of COTS distribution before outbreaks is crucial for timely intervention and effective management. However, traditional monitoring methods are time-consuming, labor-intensive, and lack the sensitivity required for early warning and proactive control. In this study, a ratiometric electrochemical DNA biosensor was constructed for the detection of environmental DNA (eDNA) from COTS. This biosensor combines a dual-signal detection mechanism with internal calibration provided by a reference probe, along with a target recycling amplification strategy mediated by exonuclease III (Exo III). The biosensor exhibited outstanding analytical performance, with a low detection limit (LOD)of 93 fM and a linear response range spanning from 300 fM to 10 nM. Moreover, digital droplet PCR (ddPCR) verified the biosensor's accuracy and reliability (<em>P</em> > 0.05). The biosensor was successfully applied to detect COTS eDNA in environmental samples collected from the Paracel Islands. Therefore, the electrochemical biosensor is promising for detecting low-density COTS individuals or larvae before outbreaks, enabling early warning and facilitating timely ecological intervention.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109162"},"PeriodicalIF":4.5,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145456978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-01DOI: 10.1016/j.bioelechem.2025.109158
Sadaf Shakeel , Mohammad Zain Khan , Mariya Shakeel
Development of highly stable, conductive, biocompatible and cost-effective cathode is crucial for scaling up microbial electrosynthesis (MES). Bimetallic cathodes have gain importance in recent times due wide application. Current study utilized a bimetallic (nickel and iron) impregnated granular activated carbon (Ni-Fe-GAC) cathode in microbial electrosynthesis. Ni-Fe-GAC cathode was prepared via treating GACs in a mixed solution of nickel chloride and iron sulphate. Deposition of metals on GAC facilitates the improved electron transfer and biofilm formation on the GAC surface, giving 1.4 times higher acetate production than plain GAC (control). Scanning electron microscopy (SEM) confirmed the well adaptation of anaerobic microbes on the surface of Ni-Fe-GAC cathode. Linear sweep voltammetry (LSV) confirmed the better electrochemical performance of Ni-Fe-GAC cathode. A regeneration test was also conducted via acid washing to remove metals from Ni-Fe-GAC followed by reloading. Regeneration successfully restored cathode performance, making Ni-Fe-GAC suitable for long-term application.
{"title":"Microbial electrosynthesis of acetic acid from carbon dioxide using a bimetallic-granular activated carbon cathode","authors":"Sadaf Shakeel , Mohammad Zain Khan , Mariya Shakeel","doi":"10.1016/j.bioelechem.2025.109158","DOIUrl":"10.1016/j.bioelechem.2025.109158","url":null,"abstract":"<div><div>Development of highly stable, conductive, biocompatible and cost-effective cathode is crucial for scaling up microbial electrosynthesis (MES). Bimetallic cathodes have gain importance in recent times due wide application. Current study utilized a bimetallic (nickel and iron) impregnated granular activated carbon (Ni-Fe-GAC) cathode in microbial electrosynthesis. Ni-Fe-GAC cathode was prepared via treating GACs in a mixed solution of nickel chloride and iron sulphate. Deposition of metals on GAC facilitates the improved electron transfer and biofilm formation on the GAC surface, giving 1.4 times higher acetate production than plain GAC (control). Scanning electron microscopy (SEM) confirmed the well adaptation of anaerobic microbes on the surface of Ni-Fe-GAC cathode. Linear sweep voltammetry (LSV) confirmed the better electrochemical performance of Ni-Fe-GAC cathode. A regeneration test was also conducted via acid washing to remove metals from Ni-Fe-GAC followed by reloading. Regeneration successfully restored cathode performance, making Ni-Fe-GAC suitable for long-term application.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109158"},"PeriodicalIF":4.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145463040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-30DOI: 10.1016/j.bioelechem.2025.109156
Dmitry Yu. Amsheev , Alexei V. Kashevskii , Igor A. Ponomarenko , Alexander Yu. Safronov , Vladlen V. Akimov , Vladimir L. Tauson , Olga Yu. Belozerova , Igor V. Klimenkov , Irina A. Shurygina , Natalya N. Dremina , Irina S. Trukhan , Evgeniya A. Lozovskaya , Alena S. Zavyalova , Sergey B. Nikiforov
An anodic polarization of the commercially pure titanium samples in 0.1 M phosphate buffer solution (pH 7.4) in the range of potentials 0.00 to 1.00 V provides an electrochemical surface modification due to controlled passivation. The titanium oxide film thickness calculated using electrochemical impedance spectroscopy falls in two ranges: 8–11 nm for experiments conducted at open circuit potentials, and 12–26 nm for experiments under conditions of anodic polarization. The maximum value of the root mean square roughness (Ra = 80 nm) was obtained using a polarization potential of 0.75 V. At the same potential, minimal interaction of rat's fibroblasts with the titanium samples surface was observed. Thus, the analysis methods used in the work made it possible to quantify the inverse relationship between the surface heterogeneity and the adhesion of model cells in vitro.
{"title":"Morphology and biological characteristics of the electrochemically modified titanium surface","authors":"Dmitry Yu. Amsheev , Alexei V. Kashevskii , Igor A. Ponomarenko , Alexander Yu. Safronov , Vladlen V. Akimov , Vladimir L. Tauson , Olga Yu. Belozerova , Igor V. Klimenkov , Irina A. Shurygina , Natalya N. Dremina , Irina S. Trukhan , Evgeniya A. Lozovskaya , Alena S. Zavyalova , Sergey B. Nikiforov","doi":"10.1016/j.bioelechem.2025.109156","DOIUrl":"10.1016/j.bioelechem.2025.109156","url":null,"abstract":"<div><div>An anodic polarization of the commercially pure titanium samples in 0.1 M phosphate buffer solution (pH 7.4) in the range of potentials 0.00 to 1.00 V provides an electrochemical surface modification due to controlled passivation. The titanium oxide film thickness calculated using electrochemical impedance spectroscopy falls in two ranges: 8–11 nm for experiments conducted at open circuit potentials, and 12–26 nm for experiments under conditions of anodic polarization. The maximum value of the root mean square roughness (R<sub>a</sub> = 80 nm) was obtained using a polarization potential of 0.75 V. At the same potential, minimal interaction of rat's fibroblasts with the titanium samples surface was observed. Thus, the analysis methods used in the work made it possible to quantify the inverse relationship between the surface heterogeneity and the adhesion of model cells <em>in vitro</em>.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109156"},"PeriodicalIF":4.5,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145457036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-29DOI: 10.1016/j.bioelechem.2025.109155
Olha Demkiv , Nataliya Stasyuk , Marcin Holdynski , Wojciech Nogala , Mykhailo Gonchar
A novel bioelectrochemical sensor has been developed for the selective detection of homovanillic acid (HVA), an important urinary biomarker of neuroblastoma. The sensor integrates green-synthesized bimetallic gold–manganese nanoparticles (nAuMnOx NPs) with laccase-like catalytic activity into a molecularly imprinted polymer (MIP) layer on a graphite electrode (MIP/nAuMnOx/GE). The nanozyme was synthesized using gallic acid as a biocompatible reducing and stabilizing agent. The MIP was formed from (3-aminopropyl)triethoxysilane as a functional monomer providing amine groups for HVA binding, tetramethyl orthosilicate as a cross-linker forming a stable silica network, and β-cyclodextrin as a supramolecular host enabling selective inclusion of the aromatic part of HVA. This composition imparted excellent molecular recognition and electrocatalytic activity. The MIP/nAuMnOx/GE sensor showed a high response at −200 mV with a limit of detection 0.12 μM and a linear range of 1–30 μM. Sensitivity reached up to 1.39 × 104 A·M−1·m−2, representing a 14-fold enhancement over natural laccase-based sensors. The sensor demonstrated outstanding selectivity toward HVA against dopamine, 5-hydroxyindoleacetic acid, vanillylmandelic acid, uric, and ascorbic acids, and enabled direct HVA quantification in untreated human urine with recovery rates of 97.5–102 %. The synergistic integration of nAuMnOx nanozyme catalysis and MIP recognition establishes a robust platform for noninvasive neuroblastoma diagnostics.
{"title":"Laccase-mimicking nAuMnOx integrated into a molecularly imprinted electrochemical sensor for selective homovanillic acid detection in human urine","authors":"Olha Demkiv , Nataliya Stasyuk , Marcin Holdynski , Wojciech Nogala , Mykhailo Gonchar","doi":"10.1016/j.bioelechem.2025.109155","DOIUrl":"10.1016/j.bioelechem.2025.109155","url":null,"abstract":"<div><div>A novel bioelectrochemical sensor has been developed for the selective detection of homovanillic acid (HVA), an important urinary biomarker of neuroblastoma. The sensor integrates green-synthesized bimetallic gold–manganese nanoparticles (nAuMnO<sub>x</sub> NPs) with laccase-like catalytic activity into a molecularly imprinted polymer (MIP) layer on a graphite electrode (MIP/nAuMnO<sub>x</sub>/GE). The nanozyme was synthesized using gallic acid as a biocompatible reducing and stabilizing agent. The MIP was formed from (3-aminopropyl)triethoxysilane as a functional monomer providing amine groups for HVA binding, tetramethyl orthosilicate as a cross-linker forming a stable silica network, and β-cyclodextrin as a supramolecular host enabling selective inclusion of the aromatic part of HVA. This composition imparted excellent molecular recognition and electrocatalytic activity. The MIP/nAuMnO<sub>x</sub>/GE sensor showed a high response at −200 mV with a limit of detection 0.12 μM and a linear range of 1–30 μM. Sensitivity reached up to 1.39 × 10<sup>4</sup> A·M<sup>−1</sup>·m<sup>−2</sup>, representing a 14-fold enhancement over natural laccase-based sensors. The sensor demonstrated outstanding selectivity toward HVA against dopamine, 5-hydroxyindoleacetic acid, vanillylmandelic acid, uric, and ascorbic acids, and enabled direct HVA quantification in untreated human urine with recovery rates of 97.5–102 %. The synergistic integration of nAuMnO<sub>x</sub> nanozyme catalysis and MIP recognition establishes a robust platform for noninvasive neuroblastoma diagnostics.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109155"},"PeriodicalIF":4.5,"publicationDate":"2025-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145457087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-28DOI: 10.1016/j.bioelechem.2025.109152
Julia Czopinska , Filip Budny , Andrzej Peplowski , Anna Sobiepanek , Marta Jarczewska
Carbon – based surfaces are becoming attractive alternative to gold transducers, which are commonly applied for development of nucleic acid sensors. However, one of the challenges is the necessity of elaboration of carbon transducers modification methods, yielding stable receptor layers that are capable of binding to target analytes. Herein, we present the studies on the elaboration of aptamer layers aimed for electrochemical detection of cytokine - tumor necrosis factor-alpha (TNF-α). This protein can serve as biomarker of wound healing processes that might be the answer to need for rapid determination of the stage of the wound that can result in selection of proper diagnosis as well as treatment strategy. We aimed on functionalization of edge – plane pyrolytic graphite as well as planar screen – printed electrodes. The aptamer layers were formed through one-step procedure using pyrene and anthracene anchor groups conjugated with aptamers. The research enabled to choose ferri/ferrocyanide redox indicator that was the source of electrochemical signal, determine the range of linear response (5–200 ng/L) with LOD of 5.07 ng/L and test the sensor against complex samples of serum or simulated wound exudate. The developed aptasensor proved to be promising solution towards non-invasive monitoring of wound healing.
{"title":"Application of aptamer – carbon hybrid materials for electrochemical detection of wound healing biomarker – TNF-α protein","authors":"Julia Czopinska , Filip Budny , Andrzej Peplowski , Anna Sobiepanek , Marta Jarczewska","doi":"10.1016/j.bioelechem.2025.109152","DOIUrl":"10.1016/j.bioelechem.2025.109152","url":null,"abstract":"<div><div>Carbon – based surfaces are becoming attractive alternative to gold transducers, which are commonly applied for development of nucleic acid sensors. However, one of the challenges is the necessity of elaboration of carbon transducers modification methods, yielding stable receptor layers that are capable of binding to target analytes. Herein, we present the studies on the elaboration of aptamer layers aimed for electrochemical detection of cytokine - tumor necrosis factor-alpha (TNF-α). This protein can serve as biomarker of wound healing processes that might be the answer to need for rapid determination of the stage of the wound that can result in selection of proper diagnosis as well as treatment strategy. We aimed on functionalization of edge – plane pyrolytic graphite as well as planar screen – printed electrodes. The aptamer layers were formed through one-step procedure using pyrene and anthracene anchor groups conjugated with aptamers. The research enabled to choose ferri/ferrocyanide redox indicator that was the source of electrochemical signal, determine the range of linear response (5–200 ng/L) with LOD of 5.07 ng/L and test the sensor against complex samples of serum or simulated wound exudate. The developed aptasensor proved to be promising solution towards non-invasive monitoring of wound healing.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109152"},"PeriodicalIF":4.5,"publicationDate":"2025-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-28DOI: 10.1016/j.bioelechem.2025.109147
Yemin Han , Haotian Yu , Manman Lv , Han Gao , Weiming Lin , Weihao Li , Mengqing Cheng , Yan Huang , Dianhuai Meng , Tian Wen , Zuhong Lu , Quanjun Liu
Bacterial infections are highly prevalent globally, and the health issues they induce often lead to numerous severe problems for human well-being, demanding timely and accurate detection strategies. Herein, We developed a universal electrochemical biosensor for pathogen screening, offering high sensitivity, specificity, rapidity, and multiplex detection. The platform integrates interdigitated electrodes for low-voltage pathogen lysis and nucleic acid release with asymmetric recombinase polymerase amplification (aRPA) to produce single-stranded DNA, simplifying extraction and reducing detection time. Screen-printed electrodes were carboxylated using diazonium salts to immobilize Fc-labeled hairpin DNA via amide bonds. Upon applying a positive voltage, amplified DNA hybridizes with the hairpin probes, distancing Fc molecules from the electrode surface and diminishing electrochemical signals, effectively eliminating false positives. Optimized conditions enabled detection sensitivities of 10 CFU/mL for Staphylococcus aureus and 5 CFU/mL for Acinetobacter baumannii. Additionally, Spiked testing in tap water, milk, and lake water demonstrated consistency with plate counting, validating the rapid system's accuracy and applicability. Remarkably, the assay time was reduced from 6 to 8 h to 25 min while maintaining pathogen specificity. This biosensor shows promise for foodborne pathogen surveillance, environmental monitoring, and point-of-care diagnostics, offering a streamlined platform for rapid, accurate pathogen identification.
{"title":"Dual-mode electro-driven biosensor: Low-voltage lysis and hybridization synergy for rapid and sensitive pathogen screening","authors":"Yemin Han , Haotian Yu , Manman Lv , Han Gao , Weiming Lin , Weihao Li , Mengqing Cheng , Yan Huang , Dianhuai Meng , Tian Wen , Zuhong Lu , Quanjun Liu","doi":"10.1016/j.bioelechem.2025.109147","DOIUrl":"10.1016/j.bioelechem.2025.109147","url":null,"abstract":"<div><div>Bacterial infections are highly prevalent globally, and the health issues they induce often lead to numerous severe problems for human well-being, demanding timely and accurate detection strategies. Herein, We developed a universal electrochemical biosensor for pathogen screening, offering high sensitivity, specificity, rapidity, and multiplex detection. The platform integrates interdigitated electrodes for low-voltage pathogen lysis and nucleic acid release with asymmetric recombinase polymerase amplification (aRPA) to produce single-stranded DNA, simplifying extraction and reducing detection time. Screen-printed electrodes were carboxylated using diazonium salts to immobilize Fc-labeled hairpin DNA via amide bonds. Upon applying a positive voltage, amplified DNA hybridizes with the hairpin probes, distancing Fc molecules from the electrode surface and diminishing electrochemical signals, effectively eliminating false positives. Optimized conditions enabled detection sensitivities of 10 CFU/mL for <em>Staphylococcus aureus</em> and 5 CFU/mL for <em>Acinetobacter baumannii</em>. Additionally, Spiked testing in tap water, milk, and lake water demonstrated consistency with plate counting, validating the rapid system's accuracy and applicability. Remarkably, the assay time was reduced from 6 to 8 h to 25 min while maintaining pathogen specificity. This biosensor shows promise for foodborne pathogen surveillance, environmental monitoring, and point-of-care diagnostics, offering a streamlined platform for rapid, accurate pathogen identification.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109147"},"PeriodicalIF":4.5,"publicationDate":"2025-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The twin microbial fuel cell powered electro-Fenton system (twin-MFCⓅEFs), combining active oxygen component and microbial metabolism, was constructed to improve the treatment process of waste activated sludge (WAS). Nevertheless, the performance and mechanism of electron transfer underlying this enhancement remain poorly understood. This study investigated the performance and mechanism of electron generation and utilization in twin-MFCⓅEFs with WAS as substrate. The higher electron generation and recovery efficiency (8.25 % of coulombic efficiency) was attributed to the higher content of amino acids (such as tryptophan), humic substances and their aromatic groups and unsaturated conjugated double bonds in the soluble organic matter, which facilitated biodegradation and electron transfer. The higher electron utilization performance (52.76 % of faraday efficiency) relied on the superior electron supply system that exhibited greater free radical oxidation. Metagenomic analysis indicated that an increased secretory capacity of glycosyltransferases (including glucosyltransferases and β-glucosidases) and a reduced activity of acetate kinase and methyl-coenzyme M reductase alpha subunit in cellular metabolic processes favored signaling and electricity production. The study focused on electron flow in twin-MFCⓅEFs and offered a promising strategy for improving the sludge treatment process.
{"title":"Electron transfer performance and mechanism in twin microbial fuel cell powered electro-Fenton system with waste activated sludge as substrate","authors":"Jiaqi Lv , Qingliang Zhao , Junqiu Jiang , Jing Ding , Liangliang Wei , Jiawen Liang","doi":"10.1016/j.bioelechem.2025.109154","DOIUrl":"10.1016/j.bioelechem.2025.109154","url":null,"abstract":"<div><div>The twin microbial fuel cell powered electro-Fenton system (twin-MFCⓅEFs), combining active oxygen component and microbial metabolism, was constructed to improve the treatment process of waste activated sludge (WAS). Nevertheless, the performance and mechanism of electron transfer underlying this enhancement remain poorly understood. This study investigated the performance and mechanism of electron generation and utilization in twin-MFCⓅEFs with WAS as substrate. The higher electron generation and recovery efficiency (8.25 % of coulombic efficiency) was attributed to the higher content of amino acids (such as tryptophan), humic substances and their aromatic groups and unsaturated conjugated double bonds in the soluble organic matter, which facilitated biodegradation and electron transfer. The higher electron utilization performance (52.76 % of faraday efficiency) relied on the superior electron supply system that exhibited greater free radical oxidation. Metagenomic analysis indicated that an increased secretory capacity of glycosyltransferases (including glucosyltransferases and β-glucosidases) and a reduced activity of acetate kinase and methyl-coenzyme M reductase alpha subunit in cellular metabolic processes favored signaling and electricity production. The study focused on electron flow in twin-MFCⓅEFs and offered a promising strategy for improving the sludge treatment process.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109154"},"PeriodicalIF":4.5,"publicationDate":"2025-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145385494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The treatment of lipid-rich wastewater using microbial fuel cells (MFCs) is often limited by the low solubility and bioavailability of hydrophobic substrates such as fat, oil, and grease (FOG). In this study, we present a sustainable and circular strategy wherein biosurfactants (BSFs) are produced from FOG using Bacillus velezensis and used to enhance FOG bioavailability and consequent degradation in MFCs. BSF production (2.3 g/L) was confirmed via foaming, drop collapse, oil displacement, CTAB-methylene blue agar, and emulsification index assays. When used to increase the bioavailability of FOG, the presence of BSFs improved all key MFC performance metrics. Notably, a 5.5-fold increase in maximum power density was observed from 0.08 to 0.44 W/m2 when BSFs were added and FOG was used as the sole organic substrate at a 0.27% (v/v) concentration. Current density (1.1 A/m2), COD removal (81.1%), and coulombic efficiency (7.9%) also improved when BSFs were present. The control MFCs operated without BSF showed significant performance deterioration, attributed to poor emulsification, substrate accumulation, and limited availability of substrate. Comparative tests using triolein as a model lipidic substrate highlighted the effectiveness of BSF-assisted FOG degradation.
{"title":"A circular valorization of fat, oil, grease (FOG): Integrating FOG-derived biosurfactant production and FOG-fed microbial fuel cells","authors":"Min-Gu Sim , Ruggero Rossi , Geon-Soo Ha , Gahyun Baek","doi":"10.1016/j.bioelechem.2025.109151","DOIUrl":"10.1016/j.bioelechem.2025.109151","url":null,"abstract":"<div><div>The treatment of lipid-rich wastewater using microbial fuel cells (MFCs) is often limited by the low solubility and bioavailability of hydrophobic substrates such as fat, oil, and grease (FOG). In this study, we present a sustainable and circular strategy wherein biosurfactants (BSFs) are produced from FOG using <em>Bacillus velezensis</em> and used to enhance FOG bioavailability and consequent degradation in MFCs. BSF production (2.3 g/L) was confirmed via foaming, drop collapse, oil displacement, CTAB-methylene blue agar, and emulsification index assays. When used to increase the bioavailability of FOG, the presence of BSFs improved all key MFC performance metrics. Notably, a 5.5-fold increase in maximum power density was observed from 0.08 to 0.44 W/m<sup>2</sup> when BSFs were added and FOG was used as the sole organic substrate at a 0.27% (v/v) concentration. Current density (1.1 A/m<sup>2</sup>), COD removal (81.1%), and coulombic efficiency (7.9%) also improved when BSFs were present. The control MFCs operated without BSF showed significant performance deterioration, attributed to poor emulsification, substrate accumulation, and limited availability of substrate. Comparative tests using triolein as a model lipidic substrate highlighted the effectiveness of BSF-assisted FOG degradation.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109151"},"PeriodicalIF":4.5,"publicationDate":"2025-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145407769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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