Bioelectrochemistry最新文献_第6页

Iron-based anodes in CW-MFCs: mechanisms and performance enhancement for contaminant removal and sulfamethoxazole elimination cw - mfc中的铁基阳极：去除污染物和磺胺甲恶唑的机制和性能增强

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-11-23 DOI: 10.1016/j.bioelechem.2025.109177

Feng Liu , Panpan Cui , Yun Zhang , Wanyou Qian , Jiancheng Zha , Lei Sun , Yijian Li , Yue Shen , Mi Deng , Jieran Xie

In recent years, the constructed wetland–microbial fuel cell (CW-MFC) had emerged as a promising technology for antibiotic removal from water, with anode material playing a critical role in system performance. This study investigated the effects of three anode materials—activated carbon (ACCW-MFC), iron-based biochar (FCW-MFC), and pyrite (PCW-MFC)—on the removal of conventional pollutants and sulfamethoxazole (SMX), power generation, and microbial community composition. Results demonstrated that FCW–MFC achieved exceptional removal rates for COD (96.4 ± 0.68 %), TP (100 %), and SMX (100 %). FCW–MFC exhibited superior resilience to shock loads, while PCW–MFC displayed peak voltages of 402 and 399 mV before and after SMX addition, respectively. Pseudomonadota was the dominant bacterial phylum in all three MFCs (ACCW–MFC, FCW–MFC, and PCW–MFC), with relative abundances of 71.34 %, 67.26 %, and 63.35 %, respectively. Notably, FCW–MFC and PCW–MFC supported substantial populations of the denitrifying genus Thauera (27.96 % and 15.58 %, respectively). The study demonstrated that iron amendment in the anode significantly enriches the electroactive microbial community and enhanced the comprehensive performance of CW–MFCs, providing an effective strategy for simultaneous wastewater treatment and energy recovery.

近年来，人工湿地微生物燃料电池（CW-MFC）作为一种很有前途的水中抗生素去除技术，其负极材料对系统性能起着至关重要的作用。本研究考察了活性炭（ACCW-MFC）、铁基生物炭（FCW-MFC）和黄铁矿（PCW-MFC）三种负极材料对常规污染物和磺胺甲恶唑（SMX）去除、发电量和微生物群落组成的影响。结果表明，FCW-MFC对COD（96.4±0.68%）、TP（100%）和SMX（100%）的去除率较高。FCW-MFC对冲击载荷的恢复能力较强，加入SMX前后PCW-MFC的峰值电压分别为402 mV和399 mV。ACCW-MFC、FCW-MFC和PCW-MFC的优势菌门均为假单胞菌，相对丰度分别为71.34%、67.26%和63.35%。值得注意的是，FCW-MFC和PCW-MFC支持大量的反硝化属Thauera（分别为27.96%和15.58%）。研究表明，阳极铁改性显著丰富了电活性微生物群落，提高了化粪池- mfc的综合性能，为污水处理和能量回收同步提供了有效的策略。

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引用次数: 0

Functionalized laser-induced graphene enabled ultrasensitive electroimmunoassay for rapid hepatitis B virus detection 功能化激光诱导石墨烯超灵敏电免疫分析法用于乙型肝炎病毒的快速检测

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-11-22 DOI: 10.1016/j.bioelechem.2025.109179

Akkapol Suea-Ngam , Tidawan Ngamyoo , Orawon Chailapakul , Nadnudda Rodthongkum , Pisit Tangkijvanich , Pumidech Puthongkham

Hepatitis B virus (HBV) infection remains a major global health challenge, necessitating the development of rapid, sensitive, and cost-effective diagnostics for disease and public health management. Herein, a high-performance electrochemical immunosensor for the ultratrace detection of hepatitis B surface antigen (HBsAg) is presented, leveraging laser-induced graphene (LIG) to address critical limitations of conventional diagnostics. A facile CO₂ laser process fabricates a porous, three-electrode graphene sensor on polyimide, which is subsequently functionalized via electrochemical oxidation to introduce carboxylic groups for covalent antibody immobilization. The assay operates on the steric-hindrance principle, where immunocomplex formation impedes redox probe diffusion, quantifiable via square wave voltammetry. The platform demonstrates exceptional analytical performance: a wide linear range (1 × 10⁻⁴ to 1 × 10¹ ng mL⁻¹), an ultra-low detection limit of 0.04 pg mL⁻¹ in buffer, and robust functionality in human serum with detection limit of 0.18 pg mL⁻¹. It also exhibits outstanding selectivity against viral interferents and strong storage stability over 30 days. Combining laboratory-level sensitivity with point-of-care practicality, this biosensor offers a promising tool for early HBV screening, particularly in resource-limited settings.

乙型肝炎病毒（HBV）感染仍然是一项重大的全球卫生挑战，需要为疾病和公共卫生管理开发快速、敏感和具有成本效益的诊断方法。本文提出了一种用于乙型肝炎表面抗原（HBsAg）超痕量检测的高性能电化学免疫传感器，利用激光诱导石墨烯（LIG）来解决传统诊断的关键局限性。一种简单的CO2激光工艺在聚酰亚胺上制备了多孔的三电极石墨烯传感器，随后通过电化学氧化将其功能化以引入羧基以固定共价抗体。该分析操作的立体位阻原理，其中免疫复合物的形成阻碍氧化还原探针扩散，可通过方波伏安法量化。该平台具有卓越的分析性能：宽线性范围（1 × 10−4至1 × 101 ng mL−1），缓冲液的超低检出限为0.04 pg mL−1，在人血清中的检测限为0.18 pg mL−1，功能强大。对病毒干扰素具有较强的选择性和30天以上的储存稳定性。结合实验室级别的灵敏度和护理点实用性，这种生物传感器为早期HBV筛查提供了一个有前途的工具，特别是在资源有限的环境中。

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引用次数: 0

Electrochemical sensor for direct HIV-1 DNA detection using ruthenium assembled on a pyrrolidinyl peptide nucleic acid-DNA hybrid 将钌组装在吡咯烷基肽核酸-DNA杂交体上，用于直接检测HIV-1 DNA的电化学传感器。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-11-22 DOI: 10.1016/j.bioelechem.2025.109172

Khanut Chittuam , Nantanat Lersanansit , Pisit Tangkijvanich , Natthaya Chuaypen , Anchalee Avihingsanon , Tirayut Vilaivan , Mohini Sain , Nattaya Ngamrojanavanich , Sakda Jampasa , Orawon Chailapakul

This study presents an electrochemical DNA biosensor designed for the direct, label-free, and sensitive detection of human immunodeficiency virus type 1 (HIV-1) DNA. The biosensor utilized pyrrolidinyl peptide nucleic acid (acpcPNA) probes immobilized on chitosan/glutaraldehyde-modified screen-printed carbon electrodes to facilitate the duplex invasion process with the target DNA. In the presence of the target DNA backbone, the formed duplex invasion triggered the electrostatic accumulation of a cationic hexaammineruthenium(III) redox indicator, resulting in an increased current response monitored by square wave voltammetry. The biosensor detected synthetic HIV-1 DNA within a linear range of 5–75 nM (limit of detection (LOD): 1.34 nM; limit of quantification (LOQ): 4.44 nM) and clinical HIV-1 cDNA within 10³–10⁵ copies/mL (LOD: 2.25 × 10² copies/mL; LOQ: 7.50 × 10² copies/mL), with correlation coefficients of 0.9988 and 0.9905, respectively. Importantly, the proposed platform eliminated the need for thermal denaturation and amplification while demonstrating strong selectivity against mismatched and non-target sequences, as confirmed by RT-PCR. In addition, this flexible platform enabled on-site analysis by integrating successfully with Bluetooth potentiostats and near-field communication reader chips. Given its advantages, this biosensor could serve as a promising prototype and a valuable tool for resource-limited settings requiring the detection of clinically relevant nucleic acid markers.

本研究提出了一种电化学DNA生物传感器，用于直接、无标记、灵敏地检测人类免疫缺陷病毒1型（HIV-1） DNA。该生物传感器利用吡咯烷酰肽核酸（acpcPNA）探针固定在壳聚糖/戊二醛修饰的丝网印刷碳电极上，以促进与目标DNA的双工侵入过程。在目标DNA主链存在的情况下，形成的双工侵入触发了阳离子六胺酸（III）氧化还原指示剂的静电积累，导致方波伏安法监测的电流响应增加。该生物传感器在5 ~ 75 nM的线性范围内检测合成HIV-1 DNA（检出限：1.34 nM）；定量限（LOQ）： 4.44 nM)和临床HIV-1 cDNA在103 ~ 105 copies/mL范围内（LOD: 2.25 × 102 copies/mL; LOQ: 7.50 × 102 copies/mL），相关系数分别为0.9988和0.9905。重要的是，该平台消除了热变性和扩增的需要，同时显示出对不匹配和非目标序列的强选择性，正如RT-PCR所证实的那样。此外，这个灵活的平台通过与蓝牙电位器和近场通信读取器芯片成功集成，实现了现场分析。鉴于其优点，这种生物传感器可以作为一个有前途的原型，并在资源有限的情况下需要检测临床相关的核酸标记物。

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引用次数: 0

Optimized hydrogel environment for a tyrosinase-based sandwich-type phenol biosensor: Application of modeling results to real samples 基于酪氨酸酶的三明治型苯酚生物传感器的优化水凝胶环境：建模结果在实际样品中的应用

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-11-22 DOI: 10.1016/j.bioelechem.2025.109176

Marianela Zoratti , Valeria Pfaffen , Fernando Garay

This work reports the development and optimization of a tyrosinase-based amperometric biosensor for the detection of oxidizable phenols in tea infusions. The enzyme was immobilized within a hydrogel matrix composed of chitosan and mucin, crosslinked with diluted glutaraldehyde. Response Surface Methodology combined with the Desirability Function was employed to optimize the enzymatic matrix, maximizing sensitivity while minimizing response time. The optimal matrix composition corresponds to 50 % chitosan and 50 % mucin crosslinked with 5 % diluted glutaraldehyde. Different enzyme loadings were evaluated to improve the trade-off between sensitivity and linear range. Experimental and theoretical results indicated that high enzyme loadings enhanced sensitivity but restricted the linear range due to oxygen depletion and promoted phenolic polymerization near the active sites, affecting stability. Conversely, lower loadings provided extended linear ranges with only a minor effect on the detection limit. An enzymatic loading of 13 U/sensor was selected as the best compromise between sensitivity, stability, and cost. The optimized biosensor enabled rapid and reproducible quantification of oxidizable phenols in green tea, black tea, and yerba mate infusions, with analysis times below one hour. Results were consistent with literature values and highlighted the necessity of applying a standard addition method to account for matrix effects.

本工作报道了一种基于酪氨酸酶的安培生物传感器的开发和优化，用于检测茶叶中可氧化酚。将酶固定在由壳聚糖和粘蛋白组成的水凝胶基质中，并与稀释的戊二醛交联。采用响应面法结合可取性函数对酶解基质进行优化，实现灵敏度最大化和响应时间最小化。最佳基质组成为50%壳聚糖和50%粘蛋白与5%稀释戊二醛交联。评估了不同的酶载量，以改善灵敏度和线性范围之间的权衡。实验和理论结果表明，高酶载量提高了灵敏度，但由于缺氧限制了线性范围，并促进了活性位点附近的酚醛聚合，影响了稳定性。相反，较低的载荷提供了扩展的线性范围，对检测极限只有很小的影响。在灵敏度、稳定性和成本之间选择了13 U/传感器的酶负载作为最佳折衷方案。优化后的生物传感器能够快速、可重复地定量测定绿茶、红茶和马黛茶中的可氧化酚，分析时间低于1小时。结果与文献值一致，并强调了应用标准加法方法来解释矩阵效应的必要性。

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引用次数: 0

Integrating cyclic voltammetry, fluorescence, and docking to elucidate DNA-Schiff-base ligand interactions 整合循环伏安法，荧光，对接阐明dna -希夫碱配体相互作用

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-11-21 DOI: 10.1016/j.bioelechem.2025.109175

Sudabeh Shokrollahi, Ahmad Amiri

Schiff-base ligands are versatile coordination compounds with notable biological and electrochemical importance and understanding their interactions with DNA is essential for exploring their potential pharmacological and sensing applications. This study investigates the binding mechanisms between twelve structurally distinct Schiff-base ligands and calf thymus DNA (ct-DNA), emphasizing the influence of electronic and steric factors on binding affinity. Fluorescence spectroscopy, cyclic voltammetry (CV), and molecular docking were combined to evaluate binding constants, quenching behavior, and molecular interactions. Fluorescence titrations revealed moderate-to-strong binding affinities (K_b = 2.07–9.61 × 10³ M⁻¹) with predominantly static quenching. Ligands containing planar aromatic systems and electron-withdrawing substituents exhibited stronger binding, whereas bulky or methoxy-substituted analogues showed weaker interactions. These observations were further supported by CV studies, showing decreased redox currents accompanied by potential shifts upon formation of DNA-ligand complex. Molecular docking supported the experimental findings, highlighting hydrogen bonding, π-π stacking, and hydrophobic interactions as dominant stabilizing forces. Overall, the integrated spectroscopic, electrochemical, and computational analyses demonstrate a clear structure-activity relationship, establishing that ligand planarity and electronic properties are key determinants of DNA-binding strength and illustrating the potential of cyclic voltammetry as a complementary tool for studying biomolecular interactions.

希夫碱配体是一种多功能的配位化合物，具有重要的生物学和电化学意义，了解它们与DNA的相互作用对于探索其潜在的药理学和传感应用至关重要。本研究探讨了12种结构不同的希夫碱基配体与小牛胸腺DNA （ct-DNA）的结合机制，强调了电子和空间因素对结合亲和力的影响。荧光光谱、循环伏安法（CV）和分子对接相结合来评估结合常数、猝灭行为和分子相互作用。荧光滴定显示了中等至强的结合亲和力（Kb = 2.07-9.61 × 103 M−1），主要是静态猝灭。含有平面芳香族体系和吸电子取代基的配体具有较强的结合，而体积大的或甲氧基取代的类似物具有较弱的相互作用。这些观察结果进一步得到CV研究的支持，表明氧化还原电流降低伴随着dna -配体复合物形成的电位变化。分子对接支持实验结果，强调氢键、π-π堆叠和疏水相互作用是主要的稳定力。总的来说，综合光谱、电化学和计算分析表明了明确的结构-活性关系，建立了配体的平面性和电子性质是dna结合强度的关键决定因素，并说明了循环伏安法作为研究生物分子相互作用的补充工具的潜力。

{"title":"Integrating cyclic voltammetry, fluorescence, and docking to elucidate DNA-Schiff-base ligand interactions","authors":"Sudabeh Shokrollahi, Ahmad Amiri","doi":"10.1016/j.bioelechem.2025.109175","DOIUrl":"10.1016/j.bioelechem.2025.109175","url":null,"abstract":"<div><div>Schiff-base ligands are versatile coordination compounds with notable biological and electrochemical importance and understanding their interactions with DNA is essential for exploring their potential pharmacological and sensing applications. This study investigates the binding mechanisms between twelve structurally distinct Schiff-base ligands and calf thymus DNA (ct-DNA), emphasizing the influence of electronic and steric factors on binding affinity. Fluorescence spectroscopy, cyclic voltammetry (CV), and molecular docking were combined to evaluate binding constants, quenching behavior, and molecular interactions. Fluorescence titrations revealed moderate-to-strong binding affinities (K<sub>b</sub> = 2.07–9.61 × 10<sup>3</sup> M<sup>−1</sup>) with predominantly static quenching. Ligands containing planar aromatic systems and electron-withdrawing substituents exhibited stronger binding, whereas bulky or methoxy-substituted analogues showed weaker interactions. These observations were further supported by CV studies, showing decreased redox currents accompanied by potential shifts upon formation of DNA-ligand complex. Molecular docking supported the experimental findings, highlighting hydrogen bonding, π-π stacking, and hydrophobic interactions as dominant stabilizing forces. Overall, the integrated spectroscopic, electrochemical, and computational analyses demonstrate a clear structure-activity relationship, establishing that ligand planarity and electronic properties are key determinants of DNA-binding strength and illustrating the potential of cyclic voltammetry as a complementary tool for studying biomolecular interactions.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109175"},"PeriodicalIF":4.5,"publicationDate":"2025-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145616678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flexible glucose biosensor via co-electrodeposition of methylene blue and glucose oxidase on carbon nanotube yarns 亚甲基蓝与葡萄糖氧化酶在碳纳米管纱线上共电沉积的柔性葡萄糖生物传感器

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-11-19 DOI: 10.1016/j.bioelechem.2025.109174

Jifan Zhao, Xiaorui He, Linghui Tang, Yue Wang, Shaoyan Wang

In this study, we report a flexible and highly sensitive glucose sensor (CNTYs-MB/GOx) for the first time based on methylene blue (MB) and glucose oxidase (GOx) co-electrodeposited onto carbon nanotube yarns (CNTYs). Unlike conventional MB/GOx coatings on planar carbon electrodes, the three-dimensional CNTY network provides a large electroactive surface area and efficient electron transport pathways, enabling dense and uniform immobilization of the MB/GOx complex. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) confirmed the effective capture and stabilization of GOx by MB and its homogeneous distribution on the CNTYs. This co-deposition strategy increased the active surface area 1.3-fold compared with physically adsorbed MB/GOx, resulting in significantly enhanced electrocatalytic activity. The sensor achieved a low detection limit of 25.2 μM, a wide linear range of 0.1–65 mM, excellent repeatability (RSD = 4.12 %), high selectivity (interference <5.4 %), and good long-term stability (86 % signal retention after 15 days). Moreover, it maintained a stable current response (RSD = 1.22 %) under repeated bending and mechanical deformation. In comparison of sequential electrodeposition and physical adsorption methods, this one-step co-electrodeposition enables MB and GOx to assemble synergistically on the CNTY surface, improving enzyme retention, increasing electroactive surface area, and facilitating efficient electron transfer. These results highlight the advantages of the CNTY-based co-electrodeposition approach and its potential for developing flexible, wearable glucose monitoring devices beyond traditional MB/GOx platforms.

在这项研究中，我们首次报道了一种基于亚甲基蓝（MB）和葡萄糖氧化酶（GOx）共电沉积在碳纳米管纱线（CNTYs）上的柔性高灵敏度葡萄糖传感器（CNTYs-MB/GOx）。与传统的平面碳电极上的MB/GOx涂层不同，三维CNTY网络提供了大的电活性表面积和高效的电子传递途径，从而实现了MB/GOx复合物的密集和均匀固定。扫描电镜（SEM）和傅里叶变换红外光谱（FT-IR）证实了MB对氧化石墨烯的有效捕获和稳定，以及其在CNTYs上的均匀分布。与物理吸附的MB/GOx相比，这种共沉积策略使活性表面积增加了1.3倍，从而显著提高了电催化活性。该传感器的检测限为25.2 μM，线性范围为0.1 ~ 65 mM，重复性好（RSD = 4.12%），选择性高（干扰率5.4%），长期稳定性好（15天后信号保留率86%）。在反复弯曲和机械变形下，保持稳定的电流响应（RSD = 1.22%）。与顺序电沉积和物理吸附方法相比，这种一步共电沉积使MB和GOx在CNTY表面协同组装，提高了酶保留率，增加了电活性表面积，促进了有效的电子转移。这些结果突出了基于cnty的共电沉积方法的优势，以及它在开发超越传统MB/GOx平台的灵活、可穿戴血糖监测设备方面的潜力。

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引用次数: 0

Electrochemical sensor for urinary H2O2 detection to aid AKI diagnosis and treatment evaluation 电化学传感器尿液H2O2检测辅助AKI诊断和治疗评价。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-11-16 DOI: 10.1016/j.bioelechem.2025.109173

Cheng Huang , Haowen Liu , Bao Jiang , Guoli Li , Xinlu Qin , Yinan Hua , Yongming Deng , Yicheng Wang , Lin Zhou

Acute kidney injury (AKI), a critical clinical syndrome marked by high incidence and mortality, is currently diagnosed mainly by serum creatinine (SCr) and blood urea nitrogen (BUN), which have high miss rates. This study innovatively proposes using urinary hydrogen peroxide (H₂O₂) concentration changes, caused by renal oxidative stress in AKI, as a new indicator for AKI risk assessment and treatment monitoring. Results from in vitro and AKI animal models show this indicator can quickly monitor AKI onset and drug effects in mice via electrochemical sensing technology based on Bi₂S₃@Cu_0.1, offering a novel approach for AKI diagnosis and rehabilitation monitoring.

急性肾损伤（Acute kidney injury， AKI）是一种发病率高、死亡率高的临床重症综合征，目前主要通过血清肌酐（SCr）和血尿素氮（BUN）进行诊断，漏检率高。本研究创新性地提出将肾氧化应激引起的尿中过氧化氢（H2O2）浓度变化作为AKI风险评估和治疗监测的新指标。体外和AKI动物模型结果表明，该指标可以通过基于Bi2S3@Cu0.1的电化学传感技术快速监测小鼠AKI的发病和药物效应，为AKI的诊断和康复监测提供了一种新的方法。

引用次数: 0

Carboxyl-functionalized covalent organic framework with precisely matched pore size achieving effective loading of cytochrome C for electrochemical biosensors 具有精确匹配孔径的羧基功能化共价有机骨架实现了电化学生物传感器细胞色素C的有效负载。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-11-14 DOI: 10.1016/j.bioelechem.2025.109169

HaoXian He , JianBing Li , JianMing Liu, LongSheng Pei, LongFei Miao, YongHai Song, LiMin Liu, Li Wang

The immobilization of enzymes is crucial for enhancing their catalytic activity and stability. Covalent organic frameworks (COF), with abundant active sites and tunable pore structures, enable effective immobilization of enzymes. Here, we designed carboxyl-functionalized COF (COF-COOH) to immobilize Cytochrome C (Cyt C), aiming to regulate the perfect pairing of the COF pore (3.67 nm) and the Cyt C dimension (2.6 nm × 3.2 nm × 3.3 nm). Meanwhile, the large amount of -COOH can increase the electrostatic and hydrogen bonding forces between COF-COOH and Cyt C. Thus, the Cyt C was efficiently loaded into COF-COOH through the post-modification method (loading efficiency = 62.37 %). The catalytic activity (k_cat/K_m) of Cyt C@COF-COOH toward H₂O₂ was significantly enhanced to 309.96 s⁻¹ M⁻¹ as compared to free Cyt C of 105.55 s⁻¹ M⁻¹. The catalytic activity of Cyt C@COF-COOH toward H₂O₂ still exceeds 80 % in some harsh environments (acetonitrile, dimethyl sulfoxide, tetrahydrofuran and 60 °C). The detection range of electrochemical H₂O₂ biosensor based on Cyt C@COF-COOH is as wide as 2.0–80 μM, and the sensitivity is as high as 0.373 μA μM⁻¹ cm⁻².

酶的固定化是提高酶的催化活性和稳定性的关键。共价有机框架（COF）具有丰富的活性位点和可调节的孔结构，可以有效地固定酶。我们设计了羧基功能化COF （COF- cooh）来固定细胞色素C (Cyt C)，旨在调节COF孔（3.67 nm）和Cyt C尺寸（2.6 nm × 3.2 nm × 3.3 nm）的完美配对。同时，大量的-COOH增加了COF-COOH与Cyt C之间的静电力和氢键力，从而通过后修饰的方法将Cyt C高效加载到COF-COOH中（加载效率为62.37%）。与游离Cyt C的105.55 s-1 M-1相比，Cyt C@COF-COOH对H2O2的催化活性（kcat/Km）显著提高至309.96 s-1 M-1。在一些恶劣环境（乙腈、二甲亚砜、四氢呋喃、60℃）下，Cyt C@COF-COOH对H2O2的催化活性仍然超过80%。基于Cyt C@COF-COOH的电化学H2O2生物传感器的检测范围为2.0 ~ 80 μM，灵敏度高达0.373 μA μM-1 cm-2。

{"title":"Carboxyl-functionalized covalent organic framework with precisely matched pore size achieving effective loading of cytochrome C for electrochemical biosensors","authors":"HaoXian He , JianBing Li , JianMing Liu, LongSheng Pei, LongFei Miao, YongHai Song, LiMin Liu, Li Wang","doi":"10.1016/j.bioelechem.2025.109169","DOIUrl":"10.1016/j.bioelechem.2025.109169","url":null,"abstract":"<div><div>The immobilization of enzymes is crucial for enhancing their catalytic activity and stability. Covalent organic frameworks (COF), with abundant active sites and tunable pore structures, enable effective immobilization of enzymes. Here, we designed carboxyl-functionalized COF (COF-COOH) to immobilize Cytochrome C (Cyt C), aiming to regulate the perfect pairing of the COF pore (3.67 nm) and the Cyt C dimension (2.6 nm × 3.2 nm × 3.3 nm). Meanwhile, the large amount of -COOH can increase the electrostatic and hydrogen bonding forces between COF-COOH and Cyt C. Thus, the Cyt C was efficiently loaded into COF-COOH through the post-modification method (loading efficiency = 62.37 %). The catalytic activity (<em>k</em><sub><em>cat</em></sub><em>/K</em><sub><em>m</em></sub>) of Cyt C@COF-COOH toward H<sub>2</sub>O<sub>2</sub> was significantly enhanced to 309.96 s<sup>−1</sup> M<sup>−1</sup> as compared to free Cyt C of 105.55 s<sup>−1</sup> M<sup>−1</sup>. The catalytic activity of Cyt C@COF-COOH toward H<sub>2</sub>O<sub>2</sub> still exceeds 80 % in some harsh environments (acetonitrile, dimethyl sulfoxide, tetrahydrofuran and 60 °C). The detection range of electrochemical H<sub>2</sub>O<sub>2</sub> biosensor based on Cyt C@COF-COOH is as wide as 2.0–80 μM, and the sensitivity is as high as 0.373 μA μM<sup>−1</sup> cm<sup>−2</sup>.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109169"},"PeriodicalIF":4.5,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145555973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effect of ternary nanoparticles ag@Zn-In2S3-(3-MPA): enhancement of catalyst electrocatalytic activity and luminophore electrochemiluminescence efficiency 三元纳米颗粒ag@Zn-In2S3-(3-MPA)的协同效应：增强催化剂电催化活性和发光团电化学发光效率

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-11-13 DOI: 10.1016/j.bioelechem.2025.109171

Shaopeng Zhang, Wenjin Liang, Wenjing Lai, Yingying Cheng, Chulei Zhao, Min Wang, Chenglin Hong, Hongling Li

Zn-In₂S₃ has attracted much attention in electrochemiluminescence (ECL) due to its electronic properties, but severe carrier recombination and low content of sulfur vacancy (Sv) limit its electrocatalytic activity in the ECL process. In this study, the catalytic performance of Zn-In₂S₃ for ECL process was enhanced by synthesizing Ag@Zn-In₂S₃-(3-MPA). The composite of Ag increased sulfur vacancies via electronic metal-support interaction (EMSI), which in turn enhanced the electrocatalytic activity of Zn-In₂S₃. Meanwhile the grafting of 3-mercaptopropionic acid (3-MPA) was to further enhance the electrocatalytic activity through bonding interaction and amorphization effect. All these improvements enhance the carrier separation efficiency and reduce their recombination, while facilitating the electron transfer during the generation of reactive oxygen species (ROS, e.g., *OH). This process further accelerates the electron transfer between luminol^•- and ROS, which significantly enhances the ECL signal of luminol. A sensor for the detection of carcinoembryonic antigen (CEA) was successfully constructed using Ag@Zn-In₂S₃-(3-MPA)/luminol as a secondary antibody marker with a wide detection range and high sensitivity. The study solves the problem of low catalytic activity of Zn-In₂S₃ and provides new ideas for the application of Zn-In₂S₃ in the fields of electrocatalysis, photocatalysis and electrochemiluminescence.

Zn-In2S3具有良好的电子性能，在电化学发光（ECL）中受到广泛关注，但严重的载流子复合和硫空位（Sv）含量低限制了其电催化活性。本研究通过合成Ag@Zn-In2S3-(3-MPA)来提高Zn-In2S3在ECL过程中的催化性能。Ag的复合材料通过电子金属-载体相互作用（EMSI）增加了硫空位，从而提高了Zn-In2S3的电催化活性。同时接枝3-巯基丙酸（3-MPA）通过成键作用和非晶化效应进一步提高了电催化活性。这些改进提高了载流子分离效率，减少了载流子的重组，同时促进了活性氧（ROS，如*OH）生成过程中的电子转移。这一过程进一步加速了鲁米诺•-与ROS之间的电子转移，显著增强了鲁米诺的ECL信号。以Ag@Zn-In2S3-(3-MPA)/鲁米诺为二抗标记物，成功构建了一种检测范围广、灵敏度高的癌胚抗原（CEA）传感器。该研究解决了Zn-In2S3催化活性低的问题，为Zn-In2S3在电催化、光催化和电化学发光等领域的应用提供了新的思路。

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引用次数: 0

Integrating amplification strategies and functional nanomaterials for advanced electrochemical biosensing of MicroRNA 集成放大策略和功能纳米材料用于先进的微rna电化学生物传感。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-11-13 DOI: 10.1016/j.bioelechem.2025.109170

Xi Liu , Minjie Yang , Dong Sun , Chengyi Lu , Yuanhui Ma , Yuqin Jiang , Ruizhuo Ouyang , Yuqing Miao

MicroRNAs (miRNAs) are crucial disease biomarkers, yet their short length, low abundance, and high sequence homology pose significant challenges for sensitive detection. Electrochemical biosensing presents a promising alternative, though effective signal amplification remains essential. This review summarizes recent advances in amplification strategies for electrochemical miRNA detection, covering nucleic acid–based techniques—such as hybridization chain reaction (HCR), rolling circle amplification (RCA), and catalytic hairpin assembly (CHA)—as well as nanomaterial-assisted approaches using metal–organic frameworks and transition metal dichalcogenides. Key mechanisms, advantages, and limitations of each method are discussed, along with performance metrics (e.g., detection limit and linear range) and emerging hybrid systems like RCA–CRISPR/Cas. Current challenges, including probe complexity and nanomaterial aggregation, are also addressed. Finally, the review highlights future directions involving multi-mechanism integration and clinical translation, offering insights for the development of highly sensitive and reliable electrochemical biosensors to advance precision medicine.

MicroRNAs （miRNAs）是重要的疾病生物标志物，但它们的短长度、低丰度和高序列同源性给敏感检测带来了重大挑战。电化学生物传感提出了一个有希望的替代方案，尽管有效的信号放大仍然是必不可少的。本文综述了电化学miRNA检测的扩增策略的最新进展，包括基于核酸的技术，如杂交链反应（HCR）、滚圈扩增（RCA）和催化发夹组装（CHA），以及利用金属有机框架和过渡金属二硫族化合物的纳米材料辅助方法。讨论了每种方法的关键机制、优势和局限性，以及性能指标（例如，检测极限和线性范围）和新兴的混合系统，如RCA-CRISPR/Cas。目前的挑战，包括探针的复杂性和纳米材料聚集，也解决了。最后，综述了多机制集成和临床转化的未来发展方向，为开发高灵敏度、高可靠性的电化学生物传感器以推进精准医疗提供参考。

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引用次数: 0