Bioelectrochemistry最新文献_第6页

An electrochemical biosensing platform based on α‑iron trioxide/ferriferrous oxide magnetic nanocomposites for early diagnosis of hepatocellular carcinoma 基于α -三氧化铁/氧化亚铁磁性纳米复合材料的肝细胞癌早期诊断电化学生物传感平台。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-06-01 Epub Date: 2025-12-15 DOI: 10.1016/j.bioelechem.2025.109203

Haoda Zhang , Hezhong Ouyang , Zishu Zhou , Tiebin Wan , Yongjin Li , Ruijiang Liu

The detection of biologically active proteins in complex biological matrices such as serum remains challenging. To enhance the sensitivity and accuracy of detecting the hepatocellular carcinoma biomarker stress-induced phosphoprotein 1 (STIP1), we proposed a label-free “Turn-off” electrochemical aptamer-based biosensing platform without requiring amplification steps. The biosensing platform integrated magnetic α-Fe₂O₃/Fe₃O₄ (α‑iron trioxide/ferriferrous oxide, αITFO) nanocomposites synthesized via urea hydrolysis followed by calcination-reduction processes and further functionalized with Au nanoparticles (NPs), achieving significantly enhanced electrochemical activity. The moderate magnetism of the αITFO MNCs enabled efficient magnetic separation, while their compatibility with the magnetic glassy carbon electrode (MGCE) allowed rapid sensor fabrication and highly sensitive detection at a low cost. Another notable characteristic of the STIP1 biosensing platform lay in its exceptional detection capabilities, exhibiting excellent sensitivity and a broad detection range of 10 pg mL⁻¹ to 10 μg mL⁻¹, with a remarkably low detection limit (LOD) of 3.29 pg mL⁻¹. Furthermore, the STIP1 biosensing platform was successfully implemented for analyzing clinical spiked serum samples, demonstrating recovery rates ranging from 102.2 % to 114.2 %. This validated its accuracy and effectiveness for applications in early disease diagnosis and prognostic evaluation.

在复杂的生物基质（如血清）中检测生物活性蛋白仍然具有挑战性。为了提高检测肝癌生物标志物应激诱导磷酸化蛋白1 （STIP1）的灵敏度和准确性，我们提出了一种无需标记的“关闭”电化学适配体生物传感平台，无需扩增步骤。该生物传感平台集成了磁性α- fe2o3 /Fe3O4 （α -三氧化铁/氧化亚铁，α itfo）纳米复合材料，该复合材料通过尿素水解-煅烧-还原工艺合成，并进一步被金纳米粒子（NPs）功能化，获得了显著增强的电化学活性。αITFO MNCs的中等磁性使其能够实现高效的磁分离，而它们与磁性玻璃碳电极（MGCE）的相容性使其能够以低成本快速制造传感器和高灵敏度检测。STIP1生物传感平台的另一个显著特点是其出色的检测能力，具有出色的灵敏度和10 pg mL-1 ~ 10 μg mL-1的广泛检测范围，极低的检出限（LOD）为3.29 pg mL-1。此外，STIP1生物传感平台已成功用于分析临床加标血清样本，其回收率为102.2%至114.2%。这证实了其在早期疾病诊断和预后评估中的准确性和有效性。

{"title":"An electrochemical biosensing platform based on α‑iron trioxide/ferriferrous oxide magnetic nanocomposites for early diagnosis of hepatocellular carcinoma","authors":"Haoda Zhang , Hezhong Ouyang , Zishu Zhou , Tiebin Wan , Yongjin Li , Ruijiang Liu","doi":"10.1016/j.bioelechem.2025.109203","DOIUrl":"10.1016/j.bioelechem.2025.109203","url":null,"abstract":"<div><div>The detection of biologically active proteins in complex biological matrices such as serum remains challenging. To enhance the sensitivity and accuracy of detecting the hepatocellular carcinoma biomarker stress-induced phosphoprotein 1 (STIP1), we proposed a label-free “Turn-off” electrochemical aptamer-based biosensing platform without requiring amplification steps. The biosensing platform integrated magnetic α-Fe<sub>2</sub>O<sub>3</sub>/Fe<sub>3</sub>O<sub>4</sub> (α‑iron trioxide/ferriferrous oxide, αITFO) nanocomposites synthesized via urea hydrolysis followed by calcination-reduction processes and further functionalized with Au nanoparticles (NPs), achieving significantly enhanced electrochemical activity. The moderate magnetism of the αITFO MNCs enabled efficient magnetic separation, while their compatibility with the magnetic glassy carbon electrode (MGCE) allowed rapid sensor fabrication and highly sensitive detection at a low cost. Another notable characteristic of the STIP1 biosensing platform lay in its exceptional detection capabilities, exhibiting excellent sensitivity and a broad detection range of 10 pg mL<sup>−1</sup> to 10 μg mL<sup>−1</sup>, with a remarkably low detection limit (LOD) of 3.29 pg mL<sup>−1</sup>. Furthermore, the STIP1 biosensing platform was successfully implemented for analyzing clinical spiked serum samples, demonstrating recovery rates ranging from 102.2 % to 114.2 %. This validated its accuracy and effectiveness for applications in early disease diagnosis and prognostic evaluation.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109203"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145773062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the nature of the electrochemical response in mediated bioanodes with yeast cells 酵母细胞介导生物阳极的电化学响应性质研究。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-06-01 Epub Date: 2025-12-13 DOI: 10.1016/j.bioelechem.2025.109197

Ekaterina V. Zolotukhina , Konstantin V. Gor'kov , Maria V. Dmitrieva , Maria G. Levchenko , Yuliya E. Silina

Herein, the electrochemical responses of Saccharomyces cerevisiae cultivated in YPD medium were tested in amperometric mode using microanalytical screen-printed electrodes modified with potassium ferricyanide and 1,10-phenanthroline-5,6-dione as mediators. The electrochemical signals obtained correlated well with the yeast growth curve.

Electrochemical analysis of the cell-free supernatants (CFSs), obtained after removing S. cerevisiae cells, indicated that the mediator response, whether using potassium ferricyanide or phenanthroline-5,6-dione, depends more on the composition of the CFSs than on the intrinsic electroactivity of the cells.

FT-IR and GC–MS investigations confirmed the formation of several electroactive compounds in the CFSs, such as hydroquinones and organohydrazines. Model electrochemical tests using ferricyanide as redox mediator indicated that quinones and organohydrazines are the most electroactive components formed during microbial activity.

These results suggest that the electrochemical response in mediated bioanodes containing living cells is primarily driven by interactions between the mediator and electroactive metabolic products, rather than by direct interactions between the mediator and the cells.

本研究采用铁氰化钾和1,10-菲罗啉-5,6-二酮修饰的微分析丝网印刷电极作为介质，在电流模式下测试了在YPD培养基中培养的酿酒酵母的电化学响应。得到的电化学信号与酵母生长曲线具有良好的相关性。去除酵母细胞后获得的无细胞上清（CFSs）的电化学分析表明，无论是使用铁氰化钾还是菲罗啉-5,6-二酮，介质响应更多地取决于CFSs的组成，而不是细胞的固有电活性。FT-IR和GC-MS研究证实在CFSs中形成了几种电活性化合物，如对苯二酚和有机肼。以铁氰化物为氧化还原介质的模型电化学试验表明，醌类和有机肼是微生物活动过程中形成的最具电活性的成分。这些结果表明，在含有活细胞的介导生物阳极中，电化学反应主要是由介质和电活性代谢产物之间的相互作用驱动的，而不是由介质和细胞之间的直接相互作用驱动的。

{"title":"On the nature of the electrochemical response in mediated bioanodes with yeast cells","authors":"Ekaterina V. Zolotukhina , Konstantin V. Gor'kov , Maria V. Dmitrieva , Maria G. Levchenko , Yuliya E. Silina","doi":"10.1016/j.bioelechem.2025.109197","DOIUrl":"10.1016/j.bioelechem.2025.109197","url":null,"abstract":"<div><div>Herein, the electrochemical responses of <em>Saccharomyces cerevisiae</em> cultivated in YPD medium were tested in amperometric mode using microanalytical screen-printed electrodes modified with potassium ferricyanide and 1,10-phenanthroline-5,6-dione as mediators. The electrochemical signals obtained correlated well with the yeast growth curve.</div><div>Electrochemical analysis of the cell-free supernatants (CFSs), obtained after removing <em>S. cerevisiae</em> cells, indicated that the mediator response, whether using potassium ferricyanide or phenanthroline-5,6-dione, depends more on the composition of the CFSs than on the intrinsic electroactivity of the cells.</div><div>FT-IR and GC–MS investigations confirmed the formation of several electroactive compounds in the CFSs, such as hydroquinones and organohydrazines. Model electrochemical tests using ferricyanide as redox mediator indicated that quinones and organohydrazines are the most electroactive components formed during microbial activity.</div><div>These results suggest that the electrochemical response in mediated bioanodes containing living cells is primarily driven by interactions between the mediator and electroactive metabolic products, rather than by direct interactions between the mediator and the cells.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109197"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145779664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phage-based dual-mode sensor using ECL and EIS for sensitive detection of Pseudomonas Aeruginosa 基于噬菌体的ECL和EIS双模传感器对铜绿假单胞菌的灵敏检测

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-06-01 Epub Date: 2025-12-31 DOI: 10.1016/j.bioelechem.2025.109212

Maryam Allahyari , Emre Dokuzparmak , Arzum Erdem , Alper AKKAYA , Bahattin Tanyolac

Pseudomonas aeruginosa is a major pathogen responsible for severe infections, particularly in immunocompromised patients and those with chronic conditions. Its increasing resistance to antibiotics underscores the urgent need for effective detection systems. This study introduces a novel biosensor based on bacteriophage (M-PAP1) in combination with MWCNT-COOH modified SPE and its application for the sensitive identification of Pseudomonas aeruginosa. The phage provides high specificity and strong target binding, while the combined ECL and EIS modes offer complementary signal outputs, enhancing analytical reliability and reducing false responses. The ECL sensor achieved LoD of 0.755 CFU ml⁻¹and demonstrated a broad linear working range of 2.28 to 10¹⁰ CFU ml⁻¹ (R²: 0.9981). In artificial urine samples, the sensor demonstrated a recovery rate of 92% to 97%, indicating its effectiveness in real biological matrices. The dual functionality for both ECL and EIS measurements highlights the system's versatility and potential for real-time clinical applications. The system also exhibited exceptional selectivity and minimal interference from non-specific bacteria such as Escherichia coli and Pseudomonas putida and Pseudomonas fluorescens. Overall, the dual-mode strategy significantly strengthens diagnostic accuracy by enabling cross-validated detection signals, offering a robust platform for rapid monitoring of P. aeruginosa in the context of rising antimicrobial resistance.

铜绿假单胞菌是造成严重感染的主要病原体，特别是在免疫功能低下患者和慢性病患者中。它对抗生素的耐药性日益增加，这突出表明迫切需要有效的检测系统。本研究介绍了一种基于噬菌体（M-PAP1）结合MWCNT-COOH修饰的SPE的新型生物传感器及其在铜绿假单胞菌敏感鉴定中的应用。噬菌体具有高特异性和强靶标结合性，而ECL和EIS结合模式提供互补的信号输出，提高了分析可靠性，减少了错误反应。ECL传感器的LoD为0.755 CFU ml - 1，线性工作范围为2.28至1010 CFU ml - 1 （R2: 0.9981）。在人工尿液样本中，该传感器的回收率为92%至97%，表明其在真实生物基质中的有效性。ECL和EIS测量的双重功能突出了系统的多功能性和实时临床应用的潜力。该系统还表现出卓越的选择性和最小的干扰非特异性细菌，如大肠杆菌、恶臭假单胞菌和荧光假单胞菌。总体而言，双模式策略通过启用交叉验证的检测信号显着增强了诊断准确性，为在抗菌素耐药性上升的背景下快速监测铜绿假单胞菌提供了一个强大的平台。

{"title":"Phage-based dual-mode sensor using ECL and EIS for sensitive detection of Pseudomonas Aeruginosa","authors":"Maryam Allahyari , Emre Dokuzparmak , Arzum Erdem , Alper AKKAYA , Bahattin Tanyolac","doi":"10.1016/j.bioelechem.2025.109212","DOIUrl":"10.1016/j.bioelechem.2025.109212","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em> is a major pathogen responsible for severe infections, particularly in immunocompromised patients and those with chronic conditions. Its increasing resistance to antibiotics underscores the urgent need for effective detection systems. This study introduces a novel biosensor based on bacteriophage (M-PAP1) in combination with MWCNT-COOH modified SPE and its application for the sensitive identification of <em>Pseudomonas aeruginosa</em>. The phage provides high specificity and strong target binding, while the combined ECL and EIS modes offer complementary signal outputs, enhancing analytical reliability and reducing false responses. The ECL sensor achieved LoD of 0.755 CFU ml<sup>−1</sup>and demonstrated a broad linear working range of 2.28 to 10<sup>10</sup> CFU ml<sup>−1</sup> (R<sup>2</sup>: 0.9981). In artificial urine samples, the sensor demonstrated a recovery rate of 92% to 97%, indicating its effectiveness in real biological matrices. The dual functionality for both ECL and EIS measurements highlights the system's versatility and potential for real-time clinical applications. The system also exhibited exceptional selectivity and minimal interference from non-specific bacteria such as <em>Escherichia coli</em> and <em>Pseudomonas putida</em> and <em>Pseudomonas fluorescens.</em> Overall, the dual-mode strategy significantly strengthens diagnostic accuracy by enabling cross-validated detection signals, offering a robust platform for rapid monitoring of <em>P. aeruginosa</em> in the context of rising antimicrobial resistance.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109212"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Minimal bamboo biochar dosing as sediment additive in sediment microbial fuel cells for bioelectricity production and benthic nutrient removal 竹炭作为沉积物微生物燃料电池中用于生物发电和底栖生物营养物去除的沉积物添加剂的最小剂量

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-06-01 Epub Date: 2025-11-29 DOI: 10.1016/j.bioelechem.2025.109182

Nurfarhana Nabila Mohd Noor, Kyunghoi Kim

Sediment resistivity limits the performance of sediment microbial fuel cells (SMFCs) by hindering mass electron transfer in the anodic region. This microcosm study evaluates the effect of bamboo biochar as a conductive additive at different dosages: SMFC-0 (0%), SMFC-0.1 (0.02%), SMFC-1 (0.2%), and SMFC-10 (2%). The study examines the current density, polarization behavior, redox activity, elemental composition, textural properties, anodic biofilm morphology and nutrient removal. The results show that 2% biochar (SMFC-10) increase sediment conductivity by 1.2-fold and reduces ohmic loss for mass electron transfer, achieving the highest power density (26.01 mW/m²) and current output (171 mA/m²). Field emission scanning electron microscopy (FESEM) analysis reveals dense anodic biofilm formation in SMFC-10, supporting higher bioelectricity generation. SMFC-10 improves pollutant removal and mitigates pollutant release into the overlying water, reducing ammonia‑nitrogen (NH₃−N) from 5 to 1 mg/L and chemical oxygen demand (COD) from 163 to 33 mg/L. High specific BET surface area (430.15 m²/g), small pore size (1.62 nm) and high carbon content (79.46%) of biochar contribute to improve performance and long-term stability (165 days) without nutrient replenishment. These findings demonstrate that bamboo biochar is a promising sediment additive to enhance SMFC power generation and water quality.

沉积物电阻率限制了沉积物微生物燃料电池（smfc）的性能，阻碍了阳极区质量电子的传递。本微观研究评价了竹炭作为导电添加剂在SMFC-0（0%）、SMFC-0.1（0.02%）、SMFC-1（0.2%）和SMFC-10（2%）不同剂量下的效果。该研究考察了电流密度、极化行为、氧化还原活性、元素组成、结构特性、阳极生物膜形态和营养物去除。结果表明，添加2%生物炭（SMFC-10）可使沉积物电导率提高1.2倍，降低质量电子传递的欧姆损失，获得最高的功率密度（26.01 mW/m2）和电流输出（171 mA/m2）。场发射扫描电镜（FESEM）分析显示SMFC-10形成致密的阳极生物膜，支持更高的生物发电量。SMFC-10改善了污染物的去除并减轻了污染物释放到上覆水，将氨氮（NH3 - N）从5 mg/L降低到1 mg/L，将化学需氧量（COD）从163 mg/L降低到33 mg/L。生物炭的高比BET表面积（430.15 m2/g）、小孔径（1.62 nm）和高含碳量（79.46%）有助于提高生产性能和长期稳定性（165天）。这些研究结果表明，竹生物炭是一种很有前途的沉淀物添加剂，可以提高SMFC发电效率和水质。

{"title":"Minimal bamboo biochar dosing as sediment additive in sediment microbial fuel cells for bioelectricity production and benthic nutrient removal","authors":"Nurfarhana Nabila Mohd Noor, Kyunghoi Kim","doi":"10.1016/j.bioelechem.2025.109182","DOIUrl":"10.1016/j.bioelechem.2025.109182","url":null,"abstract":"<div><div>Sediment resistivity limits the performance of sediment microbial fuel cells (SMFCs) by hindering mass electron transfer in the anodic region. This microcosm study evaluates the effect of bamboo biochar as a conductive additive at different dosages: SMFC-0 (0%), SMFC-0.1 (0.02%), SMFC-1 (0.2%), and SMFC-10 (2%). The study examines the current density, polarization behavior, redox activity, elemental composition, textural properties, anodic biofilm morphology and nutrient removal. The results show that 2% biochar (SMFC-10) increase sediment conductivity by 1.2-fold and reduces ohmic loss for mass electron transfer, achieving the highest power density (26.01 mW/m<sup>2</sup>) and current output (171 mA/m<sup>2</sup>). Field emission scanning electron microscopy (FESEM) analysis reveals dense anodic biofilm formation in SMFC-10, supporting higher bioelectricity generation. SMFC-10 improves pollutant removal and mitigates pollutant release into the overlying water, reducing ammonia‑nitrogen (NH<sub>3</sub>−N) from 5 to 1 mg/L and chemical oxygen demand (COD) from 163 to 33 mg/L. High specific BET surface area (430.15 m<sup>2</sup>/g), small pore size (1.62 nm) and high carbon content (79.46%) of biochar contribute to improve performance and long-term stability (165 days) without nutrient replenishment. These findings demonstrate that bamboo biochar is a promising sediment additive to enhance SMFC power generation and water quality.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109182"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145681734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical sensor for direct HIV-1 DNA detection using ruthenium assembled on a pyrrolidinyl peptide nucleic acid-DNA hybrid 将钌组装在吡咯烷基肽核酸-DNA杂交体上，用于直接检测HIV-1 DNA的电化学传感器。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-06-01 Epub Date: 2025-11-22 DOI: 10.1016/j.bioelechem.2025.109172

Khanut Chittuam , Nantanat Lersanansit , Pisit Tangkijvanich , Natthaya Chuaypen , Anchalee Avihingsanon , Tirayut Vilaivan , Mohini Sain , Nattaya Ngamrojanavanich , Sakda Jampasa , Orawon Chailapakul

This study presents an electrochemical DNA biosensor designed for the direct, label-free, and sensitive detection of human immunodeficiency virus type 1 (HIV-1) DNA. The biosensor utilized pyrrolidinyl peptide nucleic acid (acpcPNA) probes immobilized on chitosan/glutaraldehyde-modified screen-printed carbon electrodes to facilitate the duplex invasion process with the target DNA. In the presence of the target DNA backbone, the formed duplex invasion triggered the electrostatic accumulation of a cationic hexaammineruthenium(III) redox indicator, resulting in an increased current response monitored by square wave voltammetry. The biosensor detected synthetic HIV-1 DNA within a linear range of 5–75 nM (limit of detection (LOD): 1.34 nM; limit of quantification (LOQ): 4.44 nM) and clinical HIV-1 cDNA within 10³–10⁵ copies/mL (LOD: 2.25 × 10² copies/mL; LOQ: 7.50 × 10² copies/mL), with correlation coefficients of 0.9988 and 0.9905, respectively. Importantly, the proposed platform eliminated the need for thermal denaturation and amplification while demonstrating strong selectivity against mismatched and non-target sequences, as confirmed by RT-PCR. In addition, this flexible platform enabled on-site analysis by integrating successfully with Bluetooth potentiostats and near-field communication reader chips. Given its advantages, this biosensor could serve as a promising prototype and a valuable tool for resource-limited settings requiring the detection of clinically relevant nucleic acid markers.

本研究提出了一种电化学DNA生物传感器，用于直接、无标记、灵敏地检测人类免疫缺陷病毒1型（HIV-1） DNA。该生物传感器利用吡咯烷酰肽核酸（acpcPNA）探针固定在壳聚糖/戊二醛修饰的丝网印刷碳电极上，以促进与目标DNA的双工侵入过程。在目标DNA主链存在的情况下，形成的双工侵入触发了阳离子六胺酸（III）氧化还原指示剂的静电积累，导致方波伏安法监测的电流响应增加。该生物传感器在5 ~ 75 nM的线性范围内检测合成HIV-1 DNA（检出限：1.34 nM）；定量限（LOQ）： 4.44 nM)和临床HIV-1 cDNA在103 ~ 105 copies/mL范围内（LOD: 2.25 × 102 copies/mL; LOQ: 7.50 × 102 copies/mL），相关系数分别为0.9988和0.9905。重要的是，该平台消除了热变性和扩增的需要，同时显示出对不匹配和非目标序列的强选择性，正如RT-PCR所证实的那样。此外，这个灵活的平台通过与蓝牙电位器和近场通信读取器芯片成功集成，实现了现场分析。鉴于其优点，这种生物传感器可以作为一个有前途的原型，并在资源有限的情况下需要检测临床相关的核酸标记物。

{"title":"Electrochemical sensor for direct HIV-1 DNA detection using ruthenium assembled on a pyrrolidinyl peptide nucleic acid-DNA hybrid","authors":"Khanut Chittuam , Nantanat Lersanansit , Pisit Tangkijvanich , Natthaya Chuaypen , Anchalee Avihingsanon , Tirayut Vilaivan , Mohini Sain , Nattaya Ngamrojanavanich , Sakda Jampasa , Orawon Chailapakul","doi":"10.1016/j.bioelechem.2025.109172","DOIUrl":"10.1016/j.bioelechem.2025.109172","url":null,"abstract":"<div><div>This study presents an electrochemical DNA biosensor designed for the direct, label-free, and sensitive detection of human immunodeficiency virus type 1 (HIV-1) DNA. The biosensor utilized pyrrolidinyl peptide nucleic acid (acpcPNA) probes immobilized on chitosan/glutaraldehyde-modified screen-printed carbon electrodes to facilitate the duplex invasion process with the target DNA. In the presence of the target DNA backbone, the formed duplex invasion triggered the electrostatic accumulation of a cationic hexaammineruthenium(III) redox indicator, resulting in an increased current response monitored by square wave voltammetry. The biosensor detected synthetic HIV-1 DNA within a linear range of 5–75 nM (limit of detection (LOD): 1.34 nM; limit of quantification (LOQ): 4.44 nM) and clinical HIV-1 cDNA within 10<sup>3</sup>–10<sup>5</sup> copies/mL (LOD: 2.25 × 10<sup>2</sup> copies/mL; LOQ: 7.50 × 10<sup>2</sup> copies/mL), with correlation coefficients of 0.9988 and 0.9905, respectively. Importantly, the proposed platform eliminated the need for thermal denaturation and amplification while demonstrating strong selectivity against mismatched and non-target sequences, as confirmed by RT-PCR. In addition, this flexible platform enabled on-site analysis by integrating successfully with Bluetooth potentiostats and near-field communication reader chips. Given its advantages, this biosensor could serve as a promising prototype and a valuable tool for resource-limited settings requiring the detection of clinically relevant nucleic acid markers.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109172"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145601621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electropriming-mediated modulation of seed germination: Effects on morphology, water uptake, and oxidative status 电激发介导的种子萌发调控：对形态、水分吸收和氧化状态的影响

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-06-01 Epub Date: 2025-12-09 DOI: 10.1016/j.bioelechem.2025.109195

Gema Cárdenas-Flores , Erika Bustos-Bustos , Efigenia Montalvo-González , Marcelo Victorio-De los Santos , Victor M. Zamora-Gasga , Martina A. Chacón-López , Ulises M. López-García

Electropriming, the exposure of seeds to direct current electric fields (DCEF) prior to germination, is emerging as a sustainable physical priming strategy to enhance seed vigor and crop performance. In this study, we demonstrated that electropriming markedly accelerated tomato (Solanum lycopersicum) seed germination and induced early physiological adjustments. Treated seeds germinated faster, absorbed water more efficiently, and showed distinct morphological changes compared with non-primed controls. Electropriming also modulated membrane integrity, as evidenced by increased electrolyte leakage, and influenced redox metabolism, indicated by enhanced production of reactive species and activation of antioxidant enzymes. Together, these responses suggest that direct-current electropriming promotes a controlled oxidative burst that primes seeds for faster and more robust germination. Our findings highlight electropriming as a promising chemical-free approach to improve seed vigor, offering new opportunities for sustainable agriculture.

电激发是在种子萌发前将种子暴露在直流电场（DCEF）中，作为一种可持续的物理激发策略正在兴起，以提高种子活力和作物性能。在这项研究中，我们证明了电激发能显著加速番茄种子萌发并诱导早期生理调节。与未处理的对照相比，处理后的种子萌发速度更快，吸收水分效率更高，形态变化明显。电引发还调节了膜的完整性，这可以通过增加电解质泄漏来证明，并影响氧化还原代谢，这可以通过增强活性物质的产生和抗氧化酶的激活来证明。总之，这些反应表明，直流电激发促进了一种受控的氧化爆发，为种子更快、更强的发芽做好准备。我们的研究结果强调了电激发作为一种有前途的无化学物质的方法来提高种子活力，为可持续农业提供了新的机会。

{"title":"Electropriming-mediated modulation of seed germination: Effects on morphology, water uptake, and oxidative status","authors":"Gema Cárdenas-Flores , Erika Bustos-Bustos , Efigenia Montalvo-González , Marcelo Victorio-De los Santos , Victor M. Zamora-Gasga , Martina A. Chacón-López , Ulises M. López-García","doi":"10.1016/j.bioelechem.2025.109195","DOIUrl":"10.1016/j.bioelechem.2025.109195","url":null,"abstract":"<div><div>Electropriming, the exposure of seeds to direct current electric fields (DCEF) prior to germination, is emerging as a sustainable physical priming strategy to enhance seed vigor and crop performance. In this study, we demonstrated that electropriming markedly accelerated tomato (<em>Solanum lycopersicum</em>) seed germination and induced early physiological adjustments. Treated seeds germinated faster, absorbed water more efficiently, and showed distinct morphological changes compared with non-primed controls. Electropriming also modulated membrane integrity, as evidenced by increased electrolyte leakage, and influenced redox metabolism, indicated by enhanced production of reactive species and activation of antioxidant enzymes. Together, these responses suggest that direct-current electropriming promotes a controlled oxidative burst that primes seeds for faster and more robust germination. Our findings highlight electropriming as a promising chemical-free approach to improve seed vigor, offering new opportunities for sustainable agriculture.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109195"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 2D/3D nanoplatform for electrochemical diagnosis of miRNA-122 in breast cancer cell lines 乳腺癌细胞系miRNA-122电化学诊断的二维/三维纳米平台

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-06-01 Epub Date: 2025-12-22 DOI: 10.1016/j.bioelechem.2025.109209

Ali Esmaeilian , Nastaran Arab , Fatemeh Yazdian , Morteza Hosseini , Seyed Mohammad Reza Mortazavi , Mohammad Reza Ganjali

A novel, label-free electrochemical biosensor was precise engineered for the ultrasensitive and selective detection of miRNA-122, a pivotal biomarker in breast cancer diagnostics. This innovative sensing platform harnesses the exceptional conductivity of Ti₃C₂ MXene nanosheets, synergistically integrated with Au-decorated NH₂-UiO-66 metal-organic frameworks, yielding a structurally stable and electrochemically active hybrid interface. A thiolated DNA capture probe was immobilized onto the gold-modified MOF, enabling precise hybridization with the target miRNA-122. Methylene blue functioned as a robust redox indicator, exhibiting distinct electrochemical shifts upon duplex formation through π–π stacking and electrostatic interactions. The biosensor demonstrated outstanding analytical performance with dual linear response ranges (1 pM–1 nM and 1 nM–750.0 nM) and an impressively low limit of detection of 503 fM. High sequence specificity was achieved, successfully differentiating miRNA-122 from non-complementary and closely related sequences. Validation with lysates from three distinct breast cancer cell lines (MCF-7, MDA-MB-231, and SKBR3) confirmed its efficacy in complex biological matrices. Altogether, this biosensor platform offers a powerful and reliable tool for early breast cancer detection and holds substantial potential for clinical applications in miRNA-based diagnostics.

一种新型的无标记电化学生物传感器被精确设计用于超灵敏和选择性检测miRNA-122，这是乳腺癌诊断中的关键生物标志物。这种创新的传感平台利用Ti3C2 MXene纳米片的优异导电性，与au修饰的NH2-UiO-66金属有机框架协同集成，产生结构稳定且电化学活性的杂化界面。将巯基化的DNA捕获探针固定在金修饰的MOF上，实现与目标miRNA-122的精确杂交。亚甲基蓝作为一种强大的氧化还原指示剂，通过π -π堆叠和静电相互作用在双相形成时表现出明显的电化学位移。该生物传感器具有良好的双线性响应范围（1 pM-1 nM和1 nM - 750.0 nM）和令人印象深刻的低检测限503 fM。获得了高序列特异性，成功地将miRNA-122与非互补和密切相关的序列区分开来。用三种不同的乳腺癌细胞系（MCF-7、MDA-MB-231和SKBR3）的裂解物验证了其在复杂生物基质中的有效性。总之，这个生物传感器平台为早期乳腺癌检测提供了一个强大而可靠的工具，并在基于mirna的诊断中具有巨大的临床应用潜力。

{"title":"A 2D/3D nanoplatform for electrochemical diagnosis of miRNA-122 in breast cancer cell lines","authors":"Ali Esmaeilian , Nastaran Arab , Fatemeh Yazdian , Morteza Hosseini , Seyed Mohammad Reza Mortazavi , Mohammad Reza Ganjali","doi":"10.1016/j.bioelechem.2025.109209","DOIUrl":"10.1016/j.bioelechem.2025.109209","url":null,"abstract":"<div><div>A novel, label-free electrochemical biosensor was precise engineered for the ultrasensitive and selective detection of miRNA-122, a pivotal biomarker in breast cancer diagnostics. This innovative sensing platform harnesses the exceptional conductivity of Ti<sub>3</sub>C<sub>2</sub> MXene nanosheets, synergistically integrated with Au-decorated NH<sub>2</sub>-UiO-66 metal-organic frameworks, yielding a structurally stable and electrochemically active hybrid interface. A thiolated DNA capture probe was immobilized onto the gold-modified MOF, enabling precise hybridization with the target miRNA-122. Methylene blue functioned as a robust redox indicator, exhibiting distinct electrochemical shifts upon duplex formation through π–π stacking and electrostatic interactions. The biosensor demonstrated outstanding analytical performance with dual linear response ranges (1 pM–1 nM and 1 nM–750.0 nM) and an impressively low limit of detection of 503 fM. High sequence specificity was achieved, successfully differentiating miRNA-122 from non-complementary and closely related sequences. Validation with lysates from three distinct breast cancer cell lines (MCF-7, MDA-MB-231, and SKBR3) confirmed its efficacy in complex biological matrices. Altogether, this biosensor platform offers a powerful and reliable tool for early breast cancer detection and holds substantial potential for clinical applications in miRNA-based diagnostics.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109209"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Iron-based anodes in CW-MFCs: mechanisms and performance enhancement for contaminant removal and sulfamethoxazole elimination cw - mfc中的铁基阳极：去除污染物和磺胺甲恶唑的机制和性能增强

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-06-01 Epub Date: 2025-11-23 DOI: 10.1016/j.bioelechem.2025.109177

Feng Liu , Panpan Cui , Yun Zhang , Wanyou Qian , Jiancheng Zha , Lei Sun , Yijian Li , Yue Shen , Mi Deng , Jieran Xie

In recent years, the constructed wetland–microbial fuel cell (CW-MFC) had emerged as a promising technology for antibiotic removal from water, with anode material playing a critical role in system performance. This study investigated the effects of three anode materials—activated carbon (ACCW-MFC), iron-based biochar (FCW-MFC), and pyrite (PCW-MFC)—on the removal of conventional pollutants and sulfamethoxazole (SMX), power generation, and microbial community composition. Results demonstrated that FCW–MFC achieved exceptional removal rates for COD (96.4 ± 0.68 %), TP (100 %), and SMX (100 %). FCW–MFC exhibited superior resilience to shock loads, while PCW–MFC displayed peak voltages of 402 and 399 mV before and after SMX addition, respectively. Pseudomonadota was the dominant bacterial phylum in all three MFCs (ACCW–MFC, FCW–MFC, and PCW–MFC), with relative abundances of 71.34 %, 67.26 %, and 63.35 %, respectively. Notably, FCW–MFC and PCW–MFC supported substantial populations of the denitrifying genus Thauera (27.96 % and 15.58 %, respectively). The study demonstrated that iron amendment in the anode significantly enriches the electroactive microbial community and enhanced the comprehensive performance of CW–MFCs, providing an effective strategy for simultaneous wastewater treatment and energy recovery.

近年来，人工湿地微生物燃料电池（CW-MFC）作为一种很有前途的水中抗生素去除技术，其负极材料对系统性能起着至关重要的作用。本研究考察了活性炭（ACCW-MFC）、铁基生物炭（FCW-MFC）和黄铁矿（PCW-MFC）三种负极材料对常规污染物和磺胺甲恶唑（SMX）去除、发电量和微生物群落组成的影响。结果表明，FCW-MFC对COD（96.4±0.68%）、TP（100%）和SMX（100%）的去除率较高。FCW-MFC对冲击载荷的恢复能力较强，加入SMX前后PCW-MFC的峰值电压分别为402 mV和399 mV。ACCW-MFC、FCW-MFC和PCW-MFC的优势菌门均为假单胞菌，相对丰度分别为71.34%、67.26%和63.35%。值得注意的是，FCW-MFC和PCW-MFC支持大量的反硝化属Thauera（分别为27.96%和15.58%）。研究表明，阳极铁改性显著丰富了电活性微生物群落，提高了化粪池- mfc的综合性能，为污水处理和能量回收同步提供了有效的策略。

{"title":"Iron-based anodes in CW-MFCs: mechanisms and performance enhancement for contaminant removal and sulfamethoxazole elimination","authors":"Feng Liu , Panpan Cui , Yun Zhang , Wanyou Qian , Jiancheng Zha , Lei Sun , Yijian Li , Yue Shen , Mi Deng , Jieran Xie","doi":"10.1016/j.bioelechem.2025.109177","DOIUrl":"10.1016/j.bioelechem.2025.109177","url":null,"abstract":"<div><div>In recent years, the constructed wetland–microbial fuel cell (CW-MFC) had emerged as a promising technology for antibiotic removal from water, with anode material playing a critical role in system performance. This study investigated the effects of three anode materials—activated carbon (ACCW-MFC), iron-based biochar (FCW-MFC), and pyrite (PCW-MFC)—on the removal of conventional pollutants and sulfamethoxazole (SMX), power generation, and microbial community composition. Results demonstrated that FCW–MFC achieved exceptional removal rates for COD (96.4 ± 0.68 %), TP (100 %), and SMX (100 %). FCW–MFC exhibited superior resilience to shock loads, while PCW–MFC displayed peak voltages of 402 and 399 mV before and after SMX addition, respectively. <em>Pseudomonadota</em> was the dominant bacterial phylum in all three MFCs (ACCW–MFC, FCW–MFC, and PCW–MFC), with relative abundances of 71.34 %, 67.26 %, and 63.35 %, respectively. Notably, FCW–MFC and PCW–MFC supported substantial populations of the denitrifying genus <em>Thauera</em> (27.96 % and 15.58 %, respectively). The study demonstrated that iron amendment in the anode significantly enriches the electroactive microbial community and enhanced the comprehensive performance of CW–MFCs, providing an effective strategy for simultaneous wastewater treatment and energy recovery.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109177"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145616679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating cyclic voltammetry, fluorescence, and docking to elucidate DNA-Schiff-base ligand interactions 整合循环伏安法，荧光，对接阐明dna -希夫碱配体相互作用

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-06-01 Epub Date: 2025-11-21 DOI: 10.1016/j.bioelechem.2025.109175

Sudabeh Shokrollahi, Ahmad Amiri

Schiff-base ligands are versatile coordination compounds with notable biological and electrochemical importance and understanding their interactions with DNA is essential for exploring their potential pharmacological and sensing applications. This study investigates the binding mechanisms between twelve structurally distinct Schiff-base ligands and calf thymus DNA (ct-DNA), emphasizing the influence of electronic and steric factors on binding affinity. Fluorescence spectroscopy, cyclic voltammetry (CV), and molecular docking were combined to evaluate binding constants, quenching behavior, and molecular interactions. Fluorescence titrations revealed moderate-to-strong binding affinities (K_b = 2.07–9.61 × 10³ M⁻¹) with predominantly static quenching. Ligands containing planar aromatic systems and electron-withdrawing substituents exhibited stronger binding, whereas bulky or methoxy-substituted analogues showed weaker interactions. These observations were further supported by CV studies, showing decreased redox currents accompanied by potential shifts upon formation of DNA-ligand complex. Molecular docking supported the experimental findings, highlighting hydrogen bonding, π-π stacking, and hydrophobic interactions as dominant stabilizing forces. Overall, the integrated spectroscopic, electrochemical, and computational analyses demonstrate a clear structure-activity relationship, establishing that ligand planarity and electronic properties are key determinants of DNA-binding strength and illustrating the potential of cyclic voltammetry as a complementary tool for studying biomolecular interactions.

希夫碱配体是一种多功能的配位化合物，具有重要的生物学和电化学意义，了解它们与DNA的相互作用对于探索其潜在的药理学和传感应用至关重要。本研究探讨了12种结构不同的希夫碱基配体与小牛胸腺DNA （ct-DNA）的结合机制，强调了电子和空间因素对结合亲和力的影响。荧光光谱、循环伏安法（CV）和分子对接相结合来评估结合常数、猝灭行为和分子相互作用。荧光滴定显示了中等至强的结合亲和力（Kb = 2.07-9.61 × 103 M−1），主要是静态猝灭。含有平面芳香族体系和吸电子取代基的配体具有较强的结合，而体积大的或甲氧基取代的类似物具有较弱的相互作用。这些观察结果进一步得到CV研究的支持，表明氧化还原电流降低伴随着dna -配体复合物形成的电位变化。分子对接支持实验结果，强调氢键、π-π堆叠和疏水相互作用是主要的稳定力。总的来说，综合光谱、电化学和计算分析表明了明确的结构-活性关系，建立了配体的平面性和电子性质是dna结合强度的关键决定因素，并说明了循环伏安法作为研究生物分子相互作用的补充工具的潜力。

{"title":"Integrating cyclic voltammetry, fluorescence, and docking to elucidate DNA-Schiff-base ligand interactions","authors":"Sudabeh Shokrollahi, Ahmad Amiri","doi":"10.1016/j.bioelechem.2025.109175","DOIUrl":"10.1016/j.bioelechem.2025.109175","url":null,"abstract":"<div><div>Schiff-base ligands are versatile coordination compounds with notable biological and electrochemical importance and understanding their interactions with DNA is essential for exploring their potential pharmacological and sensing applications. This study investigates the binding mechanisms between twelve structurally distinct Schiff-base ligands and calf thymus DNA (ct-DNA), emphasizing the influence of electronic and steric factors on binding affinity. Fluorescence spectroscopy, cyclic voltammetry (CV), and molecular docking were combined to evaluate binding constants, quenching behavior, and molecular interactions. Fluorescence titrations revealed moderate-to-strong binding affinities (K<sub>b</sub> = 2.07–9.61 × 10<sup>3</sup> M<sup>−1</sup>) with predominantly static quenching. Ligands containing planar aromatic systems and electron-withdrawing substituents exhibited stronger binding, whereas bulky or methoxy-substituted analogues showed weaker interactions. These observations were further supported by CV studies, showing decreased redox currents accompanied by potential shifts upon formation of DNA-ligand complex. Molecular docking supported the experimental findings, highlighting hydrogen bonding, π-π stacking, and hydrophobic interactions as dominant stabilizing forces. Overall, the integrated spectroscopic, electrochemical, and computational analyses demonstrate a clear structure-activity relationship, establishing that ligand planarity and electronic properties are key determinants of DNA-binding strength and illustrating the potential of cyclic voltammetry as a complementary tool for studying biomolecular interactions.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109175"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145616678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferutinin can form transient pores in lipid membranes in the presence of magnesium or calcium ions 在镁离子或钙离子存在的情况下，阿魏素可以在脂质膜上形成短暂的孔隙。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-06-01 Epub Date: 2025-12-15 DOI: 10.1016/j.bioelechem.2025.109201

Zaret G. Denieva , Alexander M. Firsov , Ljudmila S. Khailova , Sergey A. Akimov , Elena A. Kotova , Oleg V. Batishchev , Yuri N. Antonenko

The natural phytoestrogen ferutinin acts as a divalent cation-selective ionophore, promoting the opening of Ca²⁺-activated permeability transition pore (PTP) in mitochondria. Here, we found that Mg²⁺ cations, known to suppress PTP, markedly augmented the ferutinin-induced permeabilization of rat liver mitochondria. To uncover the permeabilization mechanism, we measured calcein flow from large (LUV) and giant (GUV) unilamellar vesicles. In both cases, the ferutinin-induced calcein efflux was significantly enhanced in the presence of Ca²⁺ or Mg²⁺. Calcein influx into GUV stopped within 15-min incubation with ferutinin and Ca²⁺. In electrophysiological experiments, ferutinin caused a smooth increase in the electrical current through planar bilayer lipid membranes (BLM) in the presence of Ca²⁺ or Mg²⁺, followed by burst of fluctuations in the current at its high level. In patch-clamp BLM experiments, we detected transient channel activity upon the addition of ferutinin together with Ca²⁺, diminishing over minutes time scale. We propose permeabilization mechanism which involves adsorption of ferutinin on lipid membranes, its dimerization upon binding Mg²⁺ or Ca²⁺, leading to mechanical stress induction, and ultimately to membrane pore formation. The mechanical stress is then gradually relieved due to translocation of ferutinin dimers and lipid molecules to the opposite lipid monolayer, resulting in pore closure.

天然植物雌激素阿铁素作为一种二价阳离子选择性离子载体，促进线粒体中Ca2+活化的通透性过渡孔（PTP）的开放。在这里，我们发现Mg2+阳离子，已知抑制PTP，显著增强阿韦丁诱导的大鼠肝脏线粒体的通透性。为了揭示钙素的渗透机制，我们测量了大（LUV）和大（GUV）单层囊泡的钙素流量。在这两种情况下，阿魏素诱导的钙黄蛋白外排在Ca2+或Mg2+的存在下显着增强。钙黄蛋白内流到GUV与阿魏素和Ca2+孵育15分钟内停止。在电生理实验中，在Ca2+或Mg2+存在的情况下，阿威素引起平面双层脂质膜（BLM）电流的平滑增加，随后在其高水平时电流波动爆发。在膜片钳BLM实验中，我们检测到阿魏素与Ca2+一起添加后的瞬时通道活性，在几分钟的时间尺度上减弱。我们提出了渗透机制，包括阿威素在脂质膜上的吸附，其结合Mg2+或Ca2+二聚化，导致机械应力诱导，并最终形成膜孔。然后，由于阿魏素二聚体和脂质分子向相反的脂质单层易位，导致孔关闭，机械应力逐渐减轻。

{"title":"Ferutinin can form transient pores in lipid membranes in the presence of magnesium or calcium ions","authors":"Zaret G. Denieva , Alexander M. Firsov , Ljudmila S. Khailova , Sergey A. Akimov , Elena A. Kotova , Oleg V. Batishchev , Yuri N. Antonenko","doi":"10.1016/j.bioelechem.2025.109201","DOIUrl":"10.1016/j.bioelechem.2025.109201","url":null,"abstract":"<div><div>The natural phytoestrogen ferutinin acts as a divalent cation-selective ionophore, promoting the opening of Ca<sup>2+</sup>-activated permeability transition pore (PTP) in mitochondria. Here, we found that Mg<sup>2+</sup> cations, known to suppress PTP, markedly augmented the ferutinin-induced permeabilization of rat liver mitochondria. To uncover the permeabilization mechanism, we measured calcein flow from large (LUV) and giant (GUV) unilamellar vesicles. In both cases, the ferutinin-induced calcein efflux was significantly enhanced in the presence of Ca<sup>2+</sup> or Mg<sup>2+</sup>. Calcein influx into GUV stopped within 15-min incubation with ferutinin and Ca<sup>2+</sup>. In electrophysiological experiments, ferutinin caused a smooth increase in the electrical current through planar bilayer lipid membranes (BLM) in the presence of Ca<sup>2+</sup> or Mg<sup>2+</sup>, followed by burst of fluctuations in the current at its high level. In patch-clamp BLM experiments, we detected transient channel activity upon the addition of ferutinin together with Ca<sup>2+</sup>, diminishing over minutes time scale. We propose permeabilization mechanism which involves adsorption of ferutinin on lipid membranes, its dimerization upon binding Mg<sup>2+</sup> or Ca<sup>2+</sup>, leading to mechanical stress induction, and ultimately to membrane pore formation. The mechanical stress is then gradually relieved due to translocation of ferutinin dimers and lipid molecules to the opposite lipid monolayer, resulting in pore closure.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109201"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145779673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0