Bioelectrochemistry最新文献_第4页

The journey toward microbial photo-electrochemical biosensors: harnessing photosynthetic organisms for next-generation environmental sensing 迈向微生物光电化学生物传感器之旅：利用光合生物进行下一代环境传感。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-12-22 DOI: 10.1016/j.bioelechem.2025.109205

Rosa Maria Matteucci , Elisabetta Fanizza , Matteo Grattieri

The urgent need for cost-effective and reliable environmental monitoring systems has sparked interest in developing innovative biosensing platforms. Among these, microbial photoelectrochemical biosensors, which leverage the unique properties of photosynthetic microorganisms, have emerged as promising tools for environmental analysis. This perspective examines recent advances in microbial photoelectrochemical biosensor technology, focusing on the fundamental mechanisms of photosynthetic organisms and their integration with materials science. The current limitations in the implementation of microbial photoelectrochemical biosensors will be discussed, highlighting emerging solutions through nanomaterial integration and exploring how these biological systems can be engineered to detect environmental pollutants. Accordingly, a roadmap to transform these biological systems into practical environmental monitoring tools is presented, paving the way to unprecedented opportunities for the development of sustainable, sensitive, and targeted microbial biosensing platforms for real-world pollutant detection. To fully utilize the promise of these next-generation biosensing platforms, future research should concentrate on enhancing signal transduction and its stability over time, optimizing biointerface engineering, and encouraging interdisciplinary collaboration.

对具有成本效益和可靠的环境监测系统的迫切需求激发了人们对开发创新生物传感平台的兴趣。其中，微生物光电化学生物传感器利用光合微生物的独特特性，已成为环境分析的有前途的工具。这一视角考察了微生物光电化学生物传感器技术的最新进展，重点关注光合生物的基本机制及其与材料科学的结合。将讨论目前在微生物光电化学生物传感器实施中的限制，强调通过纳米材料集成的新兴解决方案，并探索如何设计这些生物系统来检测环境污染物。因此，本文提出了将这些生物系统转化为实际环境监测工具的路线图，为开发可持续的、敏感的、有针对性的微生物生物传感平台提供了前所未有的机会，用于现实世界的污染物检测。为了充分利用这些下一代生物传感平台的前景，未来的研究应集中在增强信号转导及其随时间的稳定性，优化生物界面工程，鼓励跨学科合作。

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引用次数: 0

Gold nanoparticle-modified graphene oxide electrochemical aptasensor for ultrasensitive detection of interferon gamma in kidney transplants 金纳米粒子修饰氧化石墨烯电化学感应传感器用于肾移植干扰素γ的超灵敏检测

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-12-22 DOI: 10.1016/j.bioelechem.2025.109207

Nashmin Hosseini , Sattar Akbari Nakhjavani , Mohammadreza Ardalan , Abdollah Salimi , Khosro Adibkia , Yadollah Omidi

The early and accurate detection of interferon gamma (IFN-γ), a key cytokine in acute cellular rejection following kidney transplantation, is essential for effective immunosuppressive management. Current methods often lack the sensitivity, speed, or simplicity for routine clinical immunomonitoring. Here, we developed a novel electrochemical aptasensor that employs a thiolated reduced graphene oxide/gold nanoparticles (TrGO–AuNPs) nanohybrid interface for IFN-γ detection. The platform integrates an anthraquinone-labeled hairpin aptamer immobilized onto the TrGO/AuNPs-modified glassy carbon electrode, through a binding-induced conformational switching mechanism to enhance signal transduction efficiency. Upon differential pulse voltammetry, the sensor exhibited a wide linear range from 0.28 to 900 pg mL⁻¹ and an ultra-low limit of detection (67 fg mL⁻¹). These analytical values surpass clinically relevant IFN-γ levels observed during acute cellular rejection. The synergistic TrGO/AuNPs combination significantly enhanced aptamer anchoring, surface conductivity, and signal resolution. The sensor demonstrated excellent selectivity in complex matrices and reliable recovery in spiked human serum, with a total analysis time of less than 45 min. To the best of our knowledge, this is the first report to utilize the TrGO–AuNPs nanohybrid architecture for electrochemical quantification of IFN-γ, offering a simplified, rapid, and sensitive strategy for point-of-care transplant immunomonitoring and highlighting the translational utility of the platform.

干扰素γ (IFN-γ)是肾移植后急性细胞排斥反应的关键细胞因子，早期准确检测干扰素γ对于有效的免疫抑制治疗至关重要。目前的方法往往缺乏敏感性，速度，或简单的常规临床免疫监测。在这里，我们开发了一种新型的电化学感应传感器，该传感器采用巯基化还原氧化石墨烯/金纳米颗粒（TrGO-AuNPs）纳米混合界面用于IFN-γ检测。该平台将蒽醌标记的发夹适配体固定在TrGO/ aunps修饰的玻璃碳电极上，通过结合诱导的构象切换机制提高信号转导效率。在差分脉冲伏安法上，传感器显示出从0.28到900 pg mL−1的宽线性范围和超低检测限（67 fg mL−1）。这些分析值超过急性细胞排斥反应期间观察到的临床相关IFN-γ水平。TrGO/AuNPs的协同组合显著增强了适体锚定、表面导电性和信号分辨率。该传感器在复杂基质中具有出色的选择性，在加标人血清中具有可靠的回收率，总分析时间小于45分钟。据我们所知，这是第一份利用TrGO-AuNPs纳米混合结构进行IFN-γ电化学定量的报告，为即时移植免疫监测提供了一种简化、快速和敏感的策略，并突出了该平台的转化效用。

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引用次数: 0

A 2D/3D nanoplatform for electrochemical diagnosis of miRNA-122 in breast cancer cell lines 乳腺癌细胞系miRNA-122电化学诊断的二维/三维纳米平台

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-12-22 DOI: 10.1016/j.bioelechem.2025.109209

Ali Esmaeilian , Nastaran Arab , Fatemeh Yazdian , Morteza Hosseini , Seyed Mohammad Reza Mortazavi , Mohammad Reza Ganjali

A novel, label-free electrochemical biosensor was precise engineered for the ultrasensitive and selective detection of miRNA-122, a pivotal biomarker in breast cancer diagnostics. This innovative sensing platform harnesses the exceptional conductivity of Ti₃C₂ MXene nanosheets, synergistically integrated with Au-decorated NH₂-UiO-66 metal-organic frameworks, yielding a structurally stable and electrochemically active hybrid interface. A thiolated DNA capture probe was immobilized onto the gold-modified MOF, enabling precise hybridization with the target miRNA-122. Methylene blue functioned as a robust redox indicator, exhibiting distinct electrochemical shifts upon duplex formation through π–π stacking and electrostatic interactions. The biosensor demonstrated outstanding analytical performance with dual linear response ranges (1 pM–1 nM and 1 nM–750.0 nM) and an impressively low limit of detection of 503 fM. High sequence specificity was achieved, successfully differentiating miRNA-122 from non-complementary and closely related sequences. Validation with lysates from three distinct breast cancer cell lines (MCF-7, MDA-MB-231, and SKBR3) confirmed its efficacy in complex biological matrices. Altogether, this biosensor platform offers a powerful and reliable tool for early breast cancer detection and holds substantial potential for clinical applications in miRNA-based diagnostics.

一种新型的无标记电化学生物传感器被精确设计用于超灵敏和选择性检测miRNA-122，这是乳腺癌诊断中的关键生物标志物。这种创新的传感平台利用Ti3C2 MXene纳米片的优异导电性，与au修饰的NH2-UiO-66金属有机框架协同集成，产生结构稳定且电化学活性的杂化界面。将巯基化的DNA捕获探针固定在金修饰的MOF上，实现与目标miRNA-122的精确杂交。亚甲基蓝作为一种强大的氧化还原指示剂，通过π -π堆叠和静电相互作用在双相形成时表现出明显的电化学位移。该生物传感器具有良好的双线性响应范围（1 pM-1 nM和1 nM - 750.0 nM）和令人印象深刻的低检测限503 fM。获得了高序列特异性，成功地将miRNA-122与非互补和密切相关的序列区分开来。用三种不同的乳腺癌细胞系（MCF-7、MDA-MB-231和SKBR3）的裂解物验证了其在复杂生物基质中的有效性。总之，这个生物传感器平台为早期乳腺癌检测提供了一个强大而可靠的工具，并在基于mirna的诊断中具有巨大的临床应用潜力。

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引用次数: 0

Wearable (bio)sensors for wound healing monitoring: recent achievements, challenges, and future directions 用于伤口愈合监测的可穿戴（生物）传感器：最近的成就、挑战和未来方向

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-12-18 DOI: 10.1016/j.bioelechem.2025.109204

J. Czopinska, I. Zaras, M. Jarczewska

Wound treatment costs are estimated to reach over 20 billion USD in the case of chronic wounds. Hence, the methods of monitoring the stage of healing are of high importance. It should be emphasized that traditionally applied methods, including visual examination as well as bacterial cell cultivation, are not precise and might be lengthy. Therefore, there is a great need for elaboration of novel approaches in controlling the healing process as well as enhancing it through novel drug delivery systems that can be realized using wearable sensors. Such an approach contributes to the minimization of patient discomfort and enables the live determination of the actual stage of wound healing. Herein, we present a critical review of current examples of such devices (presented from 2021 to 2025), as well as the characterization of basics such as the wound healing process and materials applied for sensors' fabrication. In the final part, we depict the challenges, including signal drift or nonspecific adsorption on the sensor surface, and future perspectives of the application of such noninvasive sensors in clinical diagnostics.

据估计，慢性伤口的伤口治疗费用超过200亿美元。因此，监测愈合阶段的方法是非常重要的。应该强调的是，传统应用的方法，包括目视检查和细菌细胞培养，是不精确的，可能是冗长的。因此，非常需要阐述控制愈合过程的新方法，并通过使用可穿戴传感器实现的新型药物输送系统来增强愈合过程。这种方法有助于最大限度地减少患者的不适，并使现场确定伤口愈合的实际阶段。在此，我们对此类设备的当前示例（从2021年到2025年）进行了批判性回顾，以及诸如伤口愈合过程和用于传感器制造的材料等基础特征。在最后一部分，我们描述了挑战，包括传感器表面的信号漂移或非特异性吸附，以及这种非侵入性传感器在临床诊断中应用的未来前景。

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引用次数: 0

Facile integration of NiO nanoflowers and Ketjen Black as a robust electrochemical aptasensing platform for tramadol detection in human biofluids NiO纳米花与Ketjen Black的快速集成作为人体生物体液中曲马多检测的强大电化学适体感应平台。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-12-15 DOI: 10.1016/j.bioelechem.2025.109199

Seyed Ali Akbar Sajadi, Zeynab Khorablou

Given the high potential for abuse and significant toxicological risks of tramadol (TRM), accurate monitoring of this opioid in human biofluids is of critical medico-legal importance. Despite substantial progress in analytical technologies, few biosensing platforms successfully combine high selectivity, operational simplicity, and clinical applicability. Herein, we report a novel electrochemical aptasensor based on differential pulse voltammetry (DPV), constructed using a hybrid nanocomposite of Ketjen Black (KB) and structurally disrupted nickel oxide nanoflowers (NiO NFs). The synergistic integration of conductive KB and defect-rich NiO nanostructures provides a robust matrix for stable aptamer immobilization and efficient signal amplification. The proposed aptasensor exhibited an exceptionally broad linear range (10 pM–750 nM) with an ultra-low limit of detection of 37 pM. It maintained excellent performance in complex biological matrices, including human serum, urine and saliva, offering high recovery rates and negligible cross-reactivity with structurally related opioids. Importantly, the fabrication strategy aligns with the principles of green analytical chemistry, utilizing microliter-scale reagent volumes, avoiding hazardous chemicals, and enabling a one-pot, scalable process. Collectively, these combined features underscore the platform's practical utility and ecological soundness, making it highly promising for real-time TRM detection in both clinical diagnostics and forensic investigations.

鉴于曲马多极有可能被滥用和具有重大毒理学风险，在人体生物体液中准确监测这种阿片类药物具有重要的医学法律意义。尽管分析技术取得了实质性进展，但很少有生物传感平台成功地将高选择性、操作简单性和临床适用性结合起来。在此，我们报道了一种基于差分脉冲伏安法（DPV）的新型电化学感应传感器，该传感器使用Ketjen Black （KB）和结构破坏的氧化镍纳米花（NiO NFs）的混合纳米复合材料构建。导电KB和富含缺陷的NiO纳米结构的协同集成为稳定的适配体固定和有效的信号放大提供了一个强大的矩阵。该传感器具有非常宽的线性范围（10 pM-750 nM），超低检测限为37 pM。它在复杂的生物基质（包括人血清、尿液和唾液）中保持优异的性能，具有高回收率和与结构相关的阿片类药物可忽略不计的交叉反应性。重要的是，制造策略符合绿色分析化学的原则，利用微升规模的试剂体积，避免危险化学品，并实现一锅，可扩展的过程。总的来说，这些综合功能强调了该平台的实用性和生态合理性，使其在临床诊断和法医调查中的实时TRM检测具有很大的前景。

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引用次数: 0

A bio-inspired strategy using hydrophobic bacterial EPS for the design of ZIF-8-based anti-corrosion coatings with secondary sealing function 利用疏水性细菌EPS设计具有二次密封功能的zif -8基防腐涂料的仿生策略

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-12-15 DOI: 10.1016/j.bioelechem.2025.109202

Jiajie Wu , Huanhuan Wei , Tingting Zhu , Zhen Li , Guang Li , Yuanyuan Guo , Zhuangzhuang Bi , Bo Wu , Qing Qu

Developing sustainable corrosion-resistant coatings remains a key challenge for protecting metals in marine environments. Here, we report a bio-inspired hybrid coating that integrates naturally hydrophobic soluble extracellular polymeric substances (s-EPS) derived from marine Bacillus subtilis with the metal-organic framework ZIF-8. The s-EPS@ZIF-8 composite is formed through electrostatic attraction and coordination between Zn²⁺ nodes of ZIF-8 and the functional groups (-COOH, -OH, -NH₂) of s-EPS, enabling a uniform and compact organic-inorganic network. The hydrophobicity of s-EPS originates from amphiphilic protein components such as BslA and the molecular rearrangement that exposes hydrophobic domains during film formation. This synergy produces a dense superhydrophobic layer on X70 carbon steel with a water contact angle of 131.7^°. Structural and chemical analyses confirm coherent incorporation of s-EPS within the ZIF-8 framework without compromising its crystallinity. Electrochemical tests reveal superior corrosion resistance, with inhibition efficiencies of 94.54 % after 2 days and 94.84 % after 30 days in artificial seawater. The coating functions via a cooperative formation-stabilisation-reinforcement mechanism, providing durable, self-reinforcing barrier protection. This strategy demonstrates the potential of combining naturally hydrophobic marine biopolymers with MOF frameworks for sustainable, high-performance anticorrosion coatings in marine environments.

开发可持续的耐腐蚀涂层仍然是海洋环境中保护金属的关键挑战。在这里，我们报道了一种生物启发的混合涂层，该涂层将来自海洋枯草芽孢杆菌的天然疏水可溶性细胞外聚合物（s-EPS）与金属有机框架ZIF-8结合在一起。ZIF-8的Zn2+节点与s-EPS的官能团（-COOH, -OH, -NH2）通过静电吸引和配位形成s-EPS@ZIF-8复合材料，形成均匀紧凑的有机-无机网络。s-EPS的疏水性源于两亲性蛋白组分，如BslA和在膜形成过程中暴露疏水结构域的分子重排。这种协同作用在X70碳钢表面形成致密的超疏水层，水接触角为131.7°。结构和化学分析证实了s-EPS在ZIF-8框架内的一致结合，而不影响其结晶度。电化学测试结果表明，在人工海水中，2 d和30 d的缓蚀效率分别为94.54%和94.84%。该涂层通过协同形成-稳定-加固机制发挥作用，提供持久的，自我增强的屏障保护。该策略展示了将天然疏水性海洋生物聚合物与MOF框架相结合，在海洋环境中实现可持续、高性能防腐涂层的潜力。

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引用次数: 0

An electrochemical biosensing platform based on α‑iron trioxide/ferriferrous oxide magnetic nanocomposites for early diagnosis of hepatocellular carcinoma 基于α -三氧化铁/氧化亚铁磁性纳米复合材料的肝细胞癌早期诊断电化学生物传感平台。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-12-15 DOI: 10.1016/j.bioelechem.2025.109203

Haoda Zhang , Hezhong Ouyang , Zishu Zhou , Tiebin Wan , Yongjin Li , Ruijiang Liu

The detection of biologically active proteins in complex biological matrices such as serum remains challenging. To enhance the sensitivity and accuracy of detecting the hepatocellular carcinoma biomarker stress-induced phosphoprotein 1 (STIP1), we proposed a label-free “Turn-off” electrochemical aptamer-based biosensing platform without requiring amplification steps. The biosensing platform integrated magnetic α-Fe₂O₃/Fe₃O₄ (α‑iron trioxide/ferriferrous oxide, αITFO) nanocomposites synthesized via urea hydrolysis followed by calcination-reduction processes and further functionalized with Au nanoparticles (NPs), achieving significantly enhanced electrochemical activity. The moderate magnetism of the αITFO MNCs enabled efficient magnetic separation, while their compatibility with the magnetic glassy carbon electrode (MGCE) allowed rapid sensor fabrication and highly sensitive detection at a low cost. Another notable characteristic of the STIP1 biosensing platform lay in its exceptional detection capabilities, exhibiting excellent sensitivity and a broad detection range of 10 pg mL⁻¹ to 10 μg mL⁻¹, with a remarkably low detection limit (LOD) of 3.29 pg mL⁻¹. Furthermore, the STIP1 biosensing platform was successfully implemented for analyzing clinical spiked serum samples, demonstrating recovery rates ranging from 102.2 % to 114.2 %. This validated its accuracy and effectiveness for applications in early disease diagnosis and prognostic evaluation.

在复杂的生物基质（如血清）中检测生物活性蛋白仍然具有挑战性。为了提高检测肝癌生物标志物应激诱导磷酸化蛋白1 （STIP1）的灵敏度和准确性，我们提出了一种无需标记的“关闭”电化学适配体生物传感平台，无需扩增步骤。该生物传感平台集成了磁性α- fe2o3 /Fe3O4 （α -三氧化铁/氧化亚铁，α itfo）纳米复合材料，该复合材料通过尿素水解-煅烧-还原工艺合成，并进一步被金纳米粒子（NPs）功能化，获得了显著增强的电化学活性。αITFO MNCs的中等磁性使其能够实现高效的磁分离，而它们与磁性玻璃碳电极（MGCE）的相容性使其能够以低成本快速制造传感器和高灵敏度检测。STIP1生物传感平台的另一个显著特点是其出色的检测能力，具有出色的灵敏度和10 pg mL-1 ~ 10 μg mL-1的广泛检测范围，极低的检出限（LOD）为3.29 pg mL-1。此外，STIP1生物传感平台已成功用于分析临床加标血清样本，其回收率为102.2%至114.2%。这证实了其在早期疾病诊断和预后评估中的准确性和有效性。

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引用次数: 0

Ferutinin can form transient pores in lipid membranes in the presence of magnesium or calcium ions 在镁离子或钙离子存在的情况下，阿魏素可以在脂质膜上形成短暂的孔隙。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-12-15 DOI: 10.1016/j.bioelechem.2025.109201

Zaret G. Denieva , Alexander M. Firsov , Ljudmila S. Khailova , Sergey A. Akimov , Elena A. Kotova , Oleg V. Batishchev , Yuri N. Antonenko

The natural phytoestrogen ferutinin acts as a divalent cation-selective ionophore, promoting the opening of Ca²⁺-activated permeability transition pore (PTP) in mitochondria. Here, we found that Mg²⁺ cations, known to suppress PTP, markedly augmented the ferutinin-induced permeabilization of rat liver mitochondria. To uncover the permeabilization mechanism, we measured calcein flow from large (LUV) and giant (GUV) unilamellar vesicles. In both cases, the ferutinin-induced calcein efflux was significantly enhanced in the presence of Ca²⁺ or Mg²⁺. Calcein influx into GUV stopped within 15-min incubation with ferutinin and Ca²⁺. In electrophysiological experiments, ferutinin caused a smooth increase in the electrical current through planar bilayer lipid membranes (BLM) in the presence of Ca²⁺ or Mg²⁺, followed by burst of fluctuations in the current at its high level. In patch-clamp BLM experiments, we detected transient channel activity upon the addition of ferutinin together with Ca²⁺, diminishing over minutes time scale. We propose permeabilization mechanism which involves adsorption of ferutinin on lipid membranes, its dimerization upon binding Mg²⁺ or Ca²⁺, leading to mechanical stress induction, and ultimately to membrane pore formation. The mechanical stress is then gradually relieved due to translocation of ferutinin dimers and lipid molecules to the opposite lipid monolayer, resulting in pore closure.

天然植物雌激素阿铁素作为一种二价阳离子选择性离子载体，促进线粒体中Ca2+活化的通透性过渡孔（PTP）的开放。在这里，我们发现Mg2+阳离子，已知抑制PTP，显著增强阿韦丁诱导的大鼠肝脏线粒体的通透性。为了揭示钙素的渗透机制，我们测量了大（LUV）和大（GUV）单层囊泡的钙素流量。在这两种情况下，阿魏素诱导的钙黄蛋白外排在Ca2+或Mg2+的存在下显着增强。钙黄蛋白内流到GUV与阿魏素和Ca2+孵育15分钟内停止。在电生理实验中，在Ca2+或Mg2+存在的情况下，阿威素引起平面双层脂质膜（BLM）电流的平滑增加，随后在其高水平时电流波动爆发。在膜片钳BLM实验中，我们检测到阿魏素与Ca2+一起添加后的瞬时通道活性，在几分钟的时间尺度上减弱。我们提出了渗透机制，包括阿威素在脂质膜上的吸附，其结合Mg2+或Ca2+二聚化，导致机械应力诱导，并最终形成膜孔。然后，由于阿魏素二聚体和脂质分子向相反的脂质单层易位，导致孔关闭，机械应力逐渐减轻。

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引用次数: 0

Highly sensitive detection of HER2 DNA based on enzyme-free target-triggered biped DNA walker combined with ARGET ATRP 基于无酶靶触发双足DNA行走器联合ARGET ATRP的HER2 DNA高灵敏度检测。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-12-13 DOI: 10.1016/j.bioelechem.2025.109196

Xiaohui Zhai, Xiaohua He, Fengzhi Li, Yaping Zhang, Huaixia Yang, Yanju Liu

Breast cancer (BC) is one of the most common cancers in women and a major global health challenge. Human epidermal growth factor receptor 2 (HER2) an important biomarker of BC, and its early detection is of great significance for the early diagnosis and treatment of BC patients. Here, a bipedal DNA walker was synthesized using an enzyme-free target-triggered catalytic hairpin assembly (CHA) strategy that is straightforward. And then an electrochemical DNA nanobiosensor for the ultrasensitive detection of HER2 was constructed based on the bipedal DNA walker and activators regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP), an electron-transfer regeneration catalyst with signal-amplifying effects. While moving, DNA walker operates without enzymatic involvement, consequently escaping enzymatic degradation in unfavorable environmental settings. The ARGET ATRP reaction introduces a large number of polymers into the sensing system, thus amplifying the signal. Notably, the method has a low detection limit of 0.04 pM and a wide detection range of 10⁻¹ pM to 10² nM. In addition, the biped DNA walker biosensor showed high selectivity and good reproducibility. More importantly, this strategy can be used for the detection of HER2 in human serum, proving its high practical value in biological analysis.

乳腺癌（BC）是女性最常见的癌症之一，也是一项重大的全球健康挑战。人表皮生长因子受体2 （HER2）是BC的重要生物标志物，其早期检测对BC患者的早期诊断和治疗具有重要意义。在这里，一个双足DNA行走器是使用无酶的目标触发的催化发夹组装（CHA）策略合成的，这是直接的。在此基础上，利用具有信号放大效应的电子转移再生催化剂——电子转移原子转移自由基聚合再生活化剂（ARGET ATRP）和双足DNA助行器构建了用于HER2超灵敏检测的电化学DNA纳米生物传感器。当移动时，DNA行走器在没有酶参与的情况下运行，因此在不利的环境设置中逃避酶降解。ARGET ATRP反应将大量聚合物引入传感系统，从而放大了信号。值得注意的是，该方法具有0.04 pM的低检出限和10-1 pM至102 nM的宽检出范围。此外，该生物传感器具有高选择性和良好的重现性。更重要的是，该策略可用于人血清中HER2的检测，证明了其在生物学分析中的高实用价值。

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引用次数: 0

On the nature of the electrochemical response in mediated bioanodes with yeast cells 酵母细胞介导生物阳极的电化学响应性质研究。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-12-13 DOI: 10.1016/j.bioelechem.2025.109197

Ekaterina V. Zolotukhina , Konstantin V. Gor'kov , Maria V. Dmitrieva , Maria G. Levchenko , Yuliya E. Silina

Herein, the electrochemical responses of Saccharomyces cerevisiae cultivated in YPD medium were tested in amperometric mode using microanalytical screen-printed electrodes modified with potassium ferricyanide and 1,10-phenanthroline-5,6-dione as mediators. The electrochemical signals obtained correlated well with the yeast growth curve.

Electrochemical analysis of the cell-free supernatants (CFSs), obtained after removing S. cerevisiae cells, indicated that the mediator response, whether using potassium ferricyanide or phenanthroline-5,6-dione, depends more on the composition of the CFSs than on the intrinsic electroactivity of the cells.

FT-IR and GC–MS investigations confirmed the formation of several electroactive compounds in the CFSs, such as hydroquinones and organohydrazines. Model electrochemical tests using ferricyanide as redox mediator indicated that quinones and organohydrazines are the most electroactive components formed during microbial activity.

These results suggest that the electrochemical response in mediated bioanodes containing living cells is primarily driven by interactions between the mediator and electroactive metabolic products, rather than by direct interactions between the mediator and the cells.

本研究采用铁氰化钾和1,10-菲罗啉-5,6-二酮修饰的微分析丝网印刷电极作为介质，在电流模式下测试了在YPD培养基中培养的酿酒酵母的电化学响应。得到的电化学信号与酵母生长曲线具有良好的相关性。去除酵母细胞后获得的无细胞上清（CFSs）的电化学分析表明，无论是使用铁氰化钾还是菲罗啉-5,6-二酮，介质响应更多地取决于CFSs的组成，而不是细胞的固有电活性。FT-IR和GC-MS研究证实在CFSs中形成了几种电活性化合物，如对苯二酚和有机肼。以铁氰化物为氧化还原介质的模型电化学试验表明，醌类和有机肼是微生物活动过程中形成的最具电活性的成分。这些结果表明，在含有活细胞的介导生物阳极中，电化学反应主要是由介质和电活性代谢产物之间的相互作用驱动的，而不是由介质和细胞之间的直接相互作用驱动的。

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引用次数: 0