Bioelectrochemistry最新文献_第5页

Fluorescence imaging of hydrogel-immobilised microalgae combined with simulations as a novel platform to study electropermeabilisation 水凝胶固定微藻的荧光成像与模拟相结合作为研究电渗透的新平台

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-12-11 DOI: 10.1016/j.bioelechem.2025.109200

Julia Baumgartner , Peter Bigler , Robert Axelrod , Dionys Breu , Tobias Hofmann , Byron Perez , Sing Teng Chua , Michael H.-P. Studer , Fengzheng Gao , Alexander Mathys

Cell electropermeabilisation in pulsed electric field (PEF) treatments depends on complex interactions among chamber geometry, treatment parameters, biological and environmental conditions. Traditional PEF optimisation methods, such as numerical simulations or flow cytometry (FCM), typically address only isolated aspects of these interactions. Here, we present an experimental-computational platform that combines fluorescence imaging of hydrogel-immobilised Chlamydomonas reinhardtii with electric field simulations. Thereby, local treatment intensity in complex chamber geometries can be assessed, and experimental workload is reduced by probing multiple field strengths in a single treatment. Cells embedded in a transparent hydrogel matrix were exposed to PEF using a custom grid electrode that creates spatial field gradients. After staining with Sytox Green, a marker of membrane damage, fluorescence microscopy images of the hydrogel were overlaid with simulated electric field maps. Quantitative image analysis enabled an estimation of the electropermeabilisation threshold within the semi-solid matrix and could be validated against conventional FCM in cell suspensions. During long-pulse (35 μs) exposures, some fluorescence patterns not captured by simulations highlighted the need for further investigation. Nevertheless, the platform provides a consistent, image-based method for evaluating local PEF treatment intensity and inhomogeneity, adaptable to diverse PEF chamber designs and biological systems.

脉冲电场（PEF）处理中的细胞电渗透性取决于腔室几何形状、处理参数、生物和环境条件之间复杂的相互作用。传统的PEF优化方法，如数值模拟或流式细胞术（FCM），通常只能解决这些相互作用的孤立方面。在这里，我们提出了一个实验计算平台，将水凝胶固定的莱茵衣藻的荧光成像与电场模拟相结合。因此，可以评估复杂腔室几何形状的局部处理强度，并且通过在一次处理中探测多个场强来减少实验工作量。嵌入在透明水凝胶基质中的细胞使用定制的网格电极暴露在PEF中，该电极可产生空间场梯度。用Sytox Green（一种膜损伤标记物）染色后，水凝胶的荧光显微镜图像被模拟电场图覆盖。定量图像分析可以估计半固体基质内的电渗透阈值，并可以与细胞悬浮液中的传统FCM进行验证。在长脉冲（35 μs）暴露期间，一些荧光模式没有被模拟捕获，这突出了进一步研究的必要性。然而，该平台提供了一种一致的、基于图像的方法来评估局部PEF处理强度和不均匀性，适用于不同的PEF室设计和生物系统。

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引用次数: 0

A bioelectrocatalytic glucose oxidation cascade for energy-efficient electrocatalysis applications 用于高能效电催化应用的生物电催化葡萄糖氧化级联

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-12-10 DOI: 10.1016/j.bioelechem.2025.109198

Yuxia Zhang , Yili Zhang , Yan Zheng , Hongfen Deng , Chen Li , Zhi Li , Cuixing Xu , Zhaohui Hou , Gangyong Li

Hydrogen energy and metal-air batteries, particularly zinc-air batteries (ZABs), have garnered significant interest as clean energy vectors and energy storage devices, respectively. However, their efficiency is constrained by the high overpotential and sluggish kinetics associated with the oxygen evolution reaction (OER). In this study, we propose a green and efficient bioelectrocatalytic cascade system designed to overcome the energy efficiency limitations of conventional OER. The system employs nitrogen-doped carbon nanotubes (N-CNTs) as both supporting material and electrocatalyst for immobilizing glucose oxidase (GOx) and for the in situ catalytic decomposition of H₂O₂ produced during the GOx-catalyzed oxidation of glucose. This approach not only significantly reduces the overpotentials required for water splitting and ZAB charging but also facilitates the co-production of high-value gluconic acid. Electrochemical evaluations demonstrate that the bioelectrocatalytic hydrogen evolution system achieves a current density of 10 mA cm⁻² at just 1.60 V. Furthermore, ZABs incorporating this system exhibit high power density and exceptional cycling stability. These findings underscore the potential of designing efficient and stable bifunctional bioelectrochemical catalysts as an energy-saving and high-efficiency strategy for hydrogen production and biomass valorization.

氢能电池和金属空气电池，特别是锌空气电池（ZABs）分别作为清洁能源载体和储能设备获得了极大的兴趣。然而，它们的效率受到与析氧反应（OER）相关的高过电位和缓慢动力学的限制。在这项研究中，我们提出了一种绿色高效的生物电催化级联系统，旨在克服传统OER的能源效率限制。该系统采用氮掺杂碳纳米管（N-CNTs）作为固定化葡萄糖氧化酶（GOx）和原位催化分解葡萄糖氧化过程中产生的H2O2的载体材料和电催化剂。该方法不仅显著降低了水裂解和ZAB充电所需的过电位，而且有利于协同生产高价值葡萄糖酸。电化学评价表明，生物电催化析氢系统在1.60 V下电流密度达到10 mA cm−2。此外，采用该系统的ZABs具有高功率密度和卓越的循环稳定性。这些发现强调了设计高效和稳定的双功能生物电化学催化剂作为节能和高效的制氢和生物质增值策略的潜力。

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引用次数: 0

Electropriming-mediated modulation of seed germination: Effects on morphology, water uptake, and oxidative status 电激发介导的种子萌发调控：对形态、水分吸收和氧化状态的影响

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-12-09 DOI: 10.1016/j.bioelechem.2025.109195

Gema Cárdenas-Flores , Erika Bustos-Bustos , Efigenia Montalvo-González , Marcelo Victorio-De los Santos , Victor M. Zamora-Gasga , Martina A. Chacón-López , Ulises M. López-García

Electropriming, the exposure of seeds to direct current electric fields (DCEF) prior to germination, is emerging as a sustainable physical priming strategy to enhance seed vigor and crop performance. In this study, we demonstrated that electropriming markedly accelerated tomato (Solanum lycopersicum) seed germination and induced early physiological adjustments. Treated seeds germinated faster, absorbed water more efficiently, and showed distinct morphological changes compared with non-primed controls. Electropriming also modulated membrane integrity, as evidenced by increased electrolyte leakage, and influenced redox metabolism, indicated by enhanced production of reactive species and activation of antioxidant enzymes. Together, these responses suggest that direct-current electropriming promotes a controlled oxidative burst that primes seeds for faster and more robust germination. Our findings highlight electropriming as a promising chemical-free approach to improve seed vigor, offering new opportunities for sustainable agriculture.

电激发是在种子萌发前将种子暴露在直流电场（DCEF）中，作为一种可持续的物理激发策略正在兴起，以提高种子活力和作物性能。在这项研究中，我们证明了电激发能显著加速番茄种子萌发并诱导早期生理调节。与未处理的对照相比，处理后的种子萌发速度更快，吸收水分效率更高，形态变化明显。电引发还调节了膜的完整性，这可以通过增加电解质泄漏来证明，并影响氧化还原代谢，这可以通过增强活性物质的产生和抗氧化酶的激活来证明。总之，这些反应表明，直流电激发促进了一种受控的氧化爆发，为种子更快、更强的发芽做好准备。我们的研究结果强调了电激发作为一种有前途的无化学物质的方法来提高种子活力，为可持续农业提供了新的机会。

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引用次数: 0

Sillenite-type multiferroic Bi12.5Fe0.5O19.5 decorated ReSe2 as an electrochemical platform for sensitive and selective detection of stress hormones in human biological fluids 硅长岩型多铁质Bi12.5Fe0.5O19.5修饰了ReSe2作为灵敏和选择性检测人体生物体液中应激激素的电化学平台

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-12-05 DOI: 10.1016/j.bioelechem.2025.109194

Rajalakshmi Sakthivel , Hsuan-I Chen , Akash Ashokrao Jagtap , Sayee Kannan Ramaraj , Yu-Chien Lin , Udesh Dhawan , Ting-Yu Liu , Ren-Jei Chung

Norepinephrine (NEP) or noradrenaline is a key catecholamine that functions both as a neurotransmitter and hormone, playing a pivotal role in mood regulation, cardiovascular function, and the stress response. Reliable and sensitive detection of NEP is crucial for clinical diagnosis and health monitoring. Herein, we describe a novel electrochemical sensor that enables efficient and affordable detection of NEP based on Sillenite-type multiferroic bismuth ferrite integrated onto rhenium diselenide nanosheets (Bi_12.5Fe_0.5O_19.5@ReSe₂) nanohybrid. The ReSe₂ nanosheets were synthesized by the hot injection method, and the Bi_12.5Fe_0.5O_19.5@ReSe₂ nanohybrid was synthesized using a hydrothermal route and uniformly deposited on the GCE via drop-casting. Comprehensive structural and morphological characterizations were conducted using advanced spectroscopic techniques. The electrochemical characteristics of the sensor were inspected via voltammetry and impedance analysis. The synergistic interaction within the nanocomposite enhances electrochemical performance. The sensor exhibited a linear detection response to NEP in the 0.5–993 μM range, with a detection limit of 0.112 μM and a sensitivity of 0.154 μA μM⁻¹ cm⁻². It showed excellent precision, stability, and strong resistance to interference from common biomolecules. Its successful application to NEP detection in human fluids (serum and urine) with satisfactory recovery confirms its promise for use in clinical diagnostics and real-time physiological monitoring.

去甲肾上腺素（NEP）或去甲肾上腺素是一种重要的儿茶酚胺，既是一种神经递质，也是一种激素，在情绪调节、心血管功能和应激反应中起着关键作用。可靠、灵敏的NEP检测对临床诊断和健康监测至关重要。在此，我们描述了一种新型电化学传感器，该传感器基于硅矿型多铁铋铁氧体集成到二硒化铼纳米片上（Bi12.5Fe0.5O19.5@ReSe2）纳米杂化物，能够高效且经济地检测NEP。采用热注射法制备了ReSe2纳米片，采用水热法制备了Bi12.5Fe0.5O19.5@ReSe2纳米杂化物，并通过滴铸法均匀沉积在GCE上。利用先进的光谱技术进行了全面的结构和形态表征。通过伏安法和阻抗分析对传感器的电化学特性进行了检测。纳米复合材料内部的协同作用提高了电化学性能。该传感器在0.5 ~ 993 μM范围内对NEP具有良好的线性检测响应，检测限为0.112 μM，灵敏度为0.154 μA μM−1 cm−2。该方法具有良好的精度、稳定性和较强的抗普通生物分子干扰能力。它在人体体液（血清和尿液）中NEP检测的成功应用，回收率令人满意，证实了它在临床诊断和实时生理监测中的应用前景。

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引用次数: 0

A flexible carbon nanotube field-effect transistor-based immunosensor for the selective and sensitive detection of salivary lysozyme: A biomarker of Alzheimer's disease 一种基于柔性碳纳米管场效应晶体管的免疫传感器，用于选择性和敏感检测唾液溶菌酶：阿尔茨海默病的生物标志物。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-12-02 DOI: 10.1016/j.bioelechem.2025.109193

Gulam Rabbani , Akbar Mohammad , Mohammad Ehtisham Khan , Waleed Zakri , Abrar Ahmad , Glowi Alasiri , Wahid Ali , Syed Kashif Ali , Nazim Hasan , Abdulrahman Khamaj , Jintae Lee

In the modern era, Alzheimer's disease (AD) is one of the most common causes of dementia, with its prevalence increasing over time. Collecting brain tissues for research related to neurodegenerative diseases is challenging due to the need for highly trained neurosurgeons to perform surgery and extract brain tissue. Access to human brain tissue is limited, as sampling faces technical, ethical and cost obstacles. Therefore, developing a noninvasive method to detect biomarkers for an accurate diagnosis of AD is crucial. In this study, we selected lysozyme (LYZ) as a model analyte to quantify in saliva because LYZ levels are upregulated in AD patients. Nafion-anchored carbon nanotubes (Nafion/CNTs) nanocomposite was successfully synthesized and deposited on the polyethylene terephthalate (PET) substrate and utilized in the fabrication of FET-based immunosensor, with anti-LYZ covalently immobilized. Under the optimized conditions, the immunosensor displayed a linear correlation in the range of 0.05 to 100 μg/mL with a LOD of 0.05 μg/mL. The immunosensor exhibited high specificity against interfering molecules and was effectively employed in detecting LYZ in human saliva sample, yielding satisfactory recoveries. This noninvasive method for quantifying LYZ as AD biomarker offers a straightforward and sensitive approach to detection, suggesting significant potential applications in clinical diagnostics.

在现代，阿尔茨海默病（AD）是痴呆症最常见的原因之一，其患病率随着时间的推移而增加。收集脑组织用于与神经退行性疾病相关的研究是具有挑战性的，因为需要训练有素的神经外科医生进行手术并提取脑组织。人类脑组织的获取是有限的，因为采样面临技术、伦理和成本障碍。因此，开发一种无创的方法来检测生物标志物，以准确诊断AD是至关重要的。在本研究中，我们选择溶菌酶（LYZ）作为模型分析物来量化唾液中的LYZ水平，因为AD患者的LYZ水平上调。成功地合成了固定碳纳米管（Nafion/CNTs）纳米复合材料，并将其沉积在聚对苯二甲酸乙二醇酯（PET）衬底上，用于制备抗lyz共价固定的基于fet的免疫传感器。在优化条件下，免疫传感器在0.05 ~ 100 μg/mL范围内呈线性相关，检出限为0.05 μg/mL。该免疫传感器对干扰分子具有高特异性，可有效检测人唾液样品中的LYZ，回收率高。这种无创的LYZ作为AD生物标志物的定量方法提供了一种简单、敏感的检测方法，在临床诊断中具有重要的潜在应用价值。

{"title":"A flexible carbon nanotube field-effect transistor-based immunosensor for the selective and sensitive detection of salivary lysozyme: A biomarker of Alzheimer's disease","authors":"Gulam Rabbani , Akbar Mohammad , Mohammad Ehtisham Khan , Waleed Zakri , Abrar Ahmad , Glowi Alasiri , Wahid Ali , Syed Kashif Ali , Nazim Hasan , Abdulrahman Khamaj , Jintae Lee","doi":"10.1016/j.bioelechem.2025.109193","DOIUrl":"10.1016/j.bioelechem.2025.109193","url":null,"abstract":"<div><div>In the modern era, Alzheimer's disease (AD) is one of the most common causes of dementia, with its prevalence increasing over time. Collecting brain tissues for research related to neurodegenerative diseases is challenging due to the need for highly trained neurosurgeons to perform surgery and extract brain tissue. Access to human brain tissue is limited, as sampling faces technical, ethical and cost obstacles. Therefore, developing a noninvasive method to detect biomarkers for an accurate diagnosis of AD is crucial. In this study, we selected lysozyme (LYZ) as a model analyte to quantify in saliva because LYZ levels are upregulated in AD patients. Nafion-anchored carbon nanotubes (Nafion/CNTs) nanocomposite was successfully synthesized and deposited on the polyethylene terephthalate (PET) substrate and utilized in the fabrication of FET-based immunosensor, with anti-LYZ covalently immobilized. Under the optimized conditions, the immunosensor displayed a linear correlation in the range of 0.05 to 100 μg/mL with a LOD of 0.05 μg/mL. The immunosensor exhibited high specificity against interfering molecules and was effectively employed in detecting LYZ in human saliva sample, yielding satisfactory recoveries. This noninvasive method for quantifying LYZ as AD biomarker offers a straightforward and sensitive approach to detection, suggesting significant potential applications in clinical diagnostics.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109193"},"PeriodicalIF":4.5,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145712939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroformation of Giant Unilamellar vesicles: Novel electrode design and parameters for enhanced GUVs production 巨型单层囊泡的电形成：新型电极设计和参数用于增强guv的生产。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-11-30 DOI: 10.1016/j.bioelechem.2025.109192

Davide Romanini , Miriam Di Martino , Lucia Sessa , Simona Concilio , Stefano Piotto

The formation of Giant Unilamellar Vesicles (GUVs) is a critical technology with applications in drug delivery and the study of cellular membranes. This work presents optimized electrode designs and parameters for the electroformation of GUVs. Conventional indium tin oxide (ITO) electrodes are fragile and have limited lifespans. Meanwhile, stainless steel offers mechanical robustness, reusability, and chemical stability due to its chromium oxide layer, particularly in aqueous buffers at near-neutral pH. Here, stainless steel electrodes with different geometries were tested as a cost-effective alternative. The influence of electrode shape, alternating current (AC) frequency, and applied voltage on vesicle yield and size was systematically investigated. Four chamber configurations were evaluated and optimized for electrical resistance. Broad stainless steel mesh 30 electrodes produced the highest vesicle yield, associated with larger surface area and favorable voltage–frequency combinations. Results indicate that electrode shape and electroformation parameters significantly affect GUV characteristics. Stainless steel electrodes can replace ITO electrodes, enabling robust and scalable GUV production. This approach supports applications in biophysics, drug delivery, and biosensor development while reducing material costs and improving operational durability.

巨型单层囊泡（GUVs）的形成是一项在药物传递和细胞膜研究中应用的关键技术。本文提出了guv电生成的优化电极设计和参数。传统的氧化铟锡（ITO）电极很脆弱，寿命有限。同时，由于不锈钢的氧化铬层，特别是在接近中性ph的水缓冲液中，不锈钢具有机械坚固性、可重复使用性和化学稳定性。在这里，不同几何形状的不锈钢电极作为一种经济有效的替代方案进行了测试。系统地研究了电极形状、交流频率和施加电压对囊泡产率和大小的影响。对四种腔室结构进行了电阻评估和优化。宽不锈钢网状30电极产生最高的囊泡产量，与更大的表面积和有利的电压频率组合有关。结果表明，电极形状和电形成参数对GUV特性有显著影响。不锈钢电极可以取代ITO电极，实现坚固和可扩展的GUV生产。这种方法支持生物物理学、药物输送和生物传感器开发的应用，同时降低材料成本并提高操作耐久性。

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引用次数: 0

Minimal bamboo biochar dosing as sediment additive in sediment microbial fuel cells for bioelectricity production and benthic nutrient removal 竹炭作为沉积物微生物燃料电池中用于生物发电和底栖生物营养物去除的沉积物添加剂的最小剂量

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-11-29 DOI: 10.1016/j.bioelechem.2025.109182

Nurfarhana Nabila Mohd Noor, Kyunghoi Kim

Sediment resistivity limits the performance of sediment microbial fuel cells (SMFCs) by hindering mass electron transfer in the anodic region. This microcosm study evaluates the effect of bamboo biochar as a conductive additive at different dosages: SMFC-0 (0%), SMFC-0.1 (0.02%), SMFC-1 (0.2%), and SMFC-10 (2%). The study examines the current density, polarization behavior, redox activity, elemental composition, textural properties, anodic biofilm morphology and nutrient removal. The results show that 2% biochar (SMFC-10) increase sediment conductivity by 1.2-fold and reduces ohmic loss for mass electron transfer, achieving the highest power density (26.01 mW/m²) and current output (171 mA/m²). Field emission scanning electron microscopy (FESEM) analysis reveals dense anodic biofilm formation in SMFC-10, supporting higher bioelectricity generation. SMFC-10 improves pollutant removal and mitigates pollutant release into the overlying water, reducing ammonia‑nitrogen (NH₃−N) from 5 to 1 mg/L and chemical oxygen demand (COD) from 163 to 33 mg/L. High specific BET surface area (430.15 m²/g), small pore size (1.62 nm) and high carbon content (79.46%) of biochar contribute to improve performance and long-term stability (165 days) without nutrient replenishment. These findings demonstrate that bamboo biochar is a promising sediment additive to enhance SMFC power generation and water quality.

沉积物电阻率限制了沉积物微生物燃料电池（smfc）的性能，阻碍了阳极区质量电子的传递。本微观研究评价了竹炭作为导电添加剂在SMFC-0（0%）、SMFC-0.1（0.02%）、SMFC-1（0.2%）和SMFC-10（2%）不同剂量下的效果。该研究考察了电流密度、极化行为、氧化还原活性、元素组成、结构特性、阳极生物膜形态和营养物去除。结果表明，添加2%生物炭（SMFC-10）可使沉积物电导率提高1.2倍，降低质量电子传递的欧姆损失，获得最高的功率密度（26.01 mW/m2）和电流输出（171 mA/m2）。场发射扫描电镜（FESEM）分析显示SMFC-10形成致密的阳极生物膜，支持更高的生物发电量。SMFC-10改善了污染物的去除并减轻了污染物释放到上覆水，将氨氮（NH3 - N）从5 mg/L降低到1 mg/L，将化学需氧量（COD）从163 mg/L降低到33 mg/L。生物炭的高比BET表面积（430.15 m2/g）、小孔径（1.62 nm）和高含碳量（79.46%）有助于提高生产性能和长期稳定性（165天）。这些研究结果表明，竹生物炭是一种很有前途的沉淀物添加剂，可以提高SMFC发电效率和水质。

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引用次数: 0

Voltage-induced closure of β-barrel channels as electrochemical gating 作为电化学门控的β桶通道的电压诱导闭合。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-11-25 DOI: 10.1016/j.bioelechem.2025.109181

Laidy M. Alvero-González , D. Aurora Perini , M. Lidón López , Antonio Alcaraz , María Queralt-Martín

Most β-barrel channels exhibit voltage gating, transitioning from high- to low-conducting states under high transmembrane potentials. Unlike flexible alpha-helical channels in which a physical occlusion appears, these rigid structures lack a clear gating mechanism. Using the bacterial porin OmpF from E. coli as a model system, we reveal a non-linear dependence of gating kinetics on electrolyte concentration, explained by a model based on Debye screening with high-concentration adjustments. Also, we demonstrate a large variability in low-conducting state conductances and a striking inversion in ion selectivity, switching from cationic in the high-conducting states to anionic in the low-conducting ones. Based on this and previous data, like the lack of a defined closed-state structure with major structural changes or narrowing of the pore, we hypothesize that OmpF channel closure could be understood as an electrochemical gating process. We suggest a non-steric mechanism in which low-conducting states arise from the disruption of the electrochemical gradient occurring when the external voltage causes subtle, collective reorganizations of channel residues, leading to surface dewetting at diverse locations of the channel. This model brings ideas from solid state nanopores were gating occurs without structural movements, offering a fresh perspective on β-barrel channel closure.

大多数β桶通道表现出电压门控，在高跨膜电位下从高导态过渡到低导态。与出现物理阻塞的柔性α螺旋通道不同，这些刚性结构缺乏明确的门控机制。利用大肠杆菌的细菌孔蛋白OmpF作为模型系统，我们揭示了门控动力学对电解质浓度的非线性依赖，并通过基于Debye筛选的高浓度调节模型来解释。此外，我们还证明了低导态电导率的大变异性和离子选择性的惊人反转，从高导态的阳离子切换到低导态的阴离子。基于这一数据和之前的数据，如缺乏具有主要结构变化或孔变窄的确定的闭合状态结构，我们假设OmpF通道闭合可以理解为电化学门控过程。我们提出了一种非位阻机制，在这种机制中，当外部电压引起细微的沟道残留物集体重组，导致沟道不同位置的表面脱湿时，发生电化学梯度的破坏，从而产生低导态。该模型引入了固态纳米孔的思想，即在没有结构运动的情况下发生门控，为β-桶通道关闭提供了新的视角。

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引用次数: 0

A low-voltage alternating electric field strategy against Phaeodactylum tricornutum: Anti-biofouling mechanism under electrical stimulation 低压交变电场对抗三角褐指藻的策略：电刺激下的抗生物结垢机制

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-11-25 DOI: 10.1016/j.bioelechem.2025.109180

Zhihao Zhou , Xuan Huang , Dongbai Sun , Hongying Yu , Xuzhou Jiang

Biofouling is significantly harmful to marine engineering equipment. Utilizing electric fields for biofouling prevention is a promising approach. Current research on electric field anti-biofouling mechanisms primarily focuses on high-voltage fields, while low-voltage alternating electric fields exhibit excellent anti-biofouling efficacy and potential. This study employs low-voltage alternating electric fields, characterized by low energy consumption, high controllability, and minimal environmental impact, to repel the typical fouling microorganisms (Phaeodactylum tricornutum). The parameters of electric field were optimized to achieve a repulsion efficiency of 97.2 %. With DCFH-DA fluorescent probes, the elevated intracellular reactive oxygen species (ROS) levels were observed after electric field treatment. Increased malondialdehyde (MDA) content confirmed the lipid peroxidation, revealing the induction of oxidative stress. Rhodamine 123 staining revealed the damage to the mitochondrial membrane in alternating electric field-treated cells, directly resulting in reduced adenosine triphosphate (ATP) levels. These results indicate that low-voltage alternating electric fields disrupts energy homeostasis via ROS-mediated mitochondrial damage, thereby inhibiting diatom adhesion. This study investigates the biological mechanism of an electric field-based anti-biofouling strategy from the perspective of cellular energy supply, highlighting the critical role of energy pathways in microbial adhesion. The findings provide theoretical support for developing next-generation anti-biofouling systems and advancing sustainable marine engineering.

生物污染对海洋工程设备的危害很大。利用电场预防生物污染是一种很有前途的方法。目前对电场抗生物污垢机理的研究主要集中在高压电场，而低压交变电场具有良好的抗生物污垢效果和潜力。本研究采用低能耗、高可控性、对环境影响小的低压交变电场，对典型的污染微生物（三角褐指藻）进行击退。对电场参数进行优化，排斥力达到97.2%。用DCFH-DA荧光探针观察电场处理后细胞内活性氧（ROS）水平升高。丙二醛（MDA）含量的增加证实了脂质过氧化，揭示了氧化应激的诱导。罗丹明123染色显示交变电场处理细胞线粒体膜损伤，直接导致三磷酸腺苷（ATP）水平降低。这些结果表明，低压交变电场通过ros介导的线粒体损伤破坏能量稳态，从而抑制硅藻粘附。本研究从细胞能量供应的角度探讨了基于电场的抗生物污垢策略的生物学机制，强调了能量途径在微生物粘附中的关键作用。研究结果为开发下一代抗生物污染系统和推进可持续海洋工程提供了理论支持。

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引用次数: 0

Interfacing nitrogen biochemistry with electrochemical output in Saccharomyces cerevisiae microbial fuel cells 酿酒酵母微生物燃料电池中氮生化与电化学输出的界面研究

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-11-23 DOI: 10.1016/j.bioelechem.2025.109178

Marcelinus Christwardana , Muhammad Fahrul Riza , Purbowatiningrum Ria Sarjono

Microbial fuel cells (MFCs) utilize microbial metabolism to convert organic substrates into electrical energy. Saccharomyces cerevisiae presents a promising eukaryotic biocatalyst due to its fermentative capacity and non-pathogenic nature, yet its electron transfer efficiency remains a major bottleneck. This study evaluates the influence of nitrogen source variation, peptone, tryptone, and bovine serum albumin (BSA) at concentrations of 1, 2.5, and 5 mg.mL⁻¹ on the electrochemical performance of Saccharomyces cerevisiae-based MFCs. Half-cell analyses, including cyclic voltammetry and rate-determining step (RDS) assessments, revealed diffusion-controlled electron transfer via cytochromes. The highest electron transfer rate constant (K_s) was obtained with peptone 5 mg.mL⁻¹ (1.61 ± 0.285 s⁻¹), followed by tryptone 1 mg.mL⁻¹ (1.53 ± 0.332 s⁻¹) and BSA 1 mg.mL⁻¹ (0.95 ± 0.055 s⁻¹). Full-cell experiments showed maximum voltage outputs of 0.132 V (peptone 5 mg.mL⁻¹), 0.117 V (tryptone 1 mg.mL⁻¹), and 0.039 V (BSA 1 mg.mL⁻¹), and corresponding peak power densities of 46.6, 44.0, and 7.1 mW m⁻². SEM confirmed enhanced biofilm formation with increased nitrogen concentration, supporting stronger electrochemical activity. These results highlight nitrogen source optimization as a strategic approach to enhance microbial electron transfer and energy yield in yeast-based MFC systems.

微生物燃料电池（MFCs）利用微生物代谢将有机底物转化为电能。酿酒酵母因其具有良好的发酵能力和非致病性而成为一种很有发展前景的真核生物催化剂，但其电子传递效率一直是制约其发展的主要瓶颈。本研究评估了氮源变化、蛋白胨、色氨酸和牛血清白蛋白（BSA）在1、2.5和5 mg.mL−1浓度下对基于酵母的mfc电化学性能的影响。半细胞分析，包括循环伏安法和速率决定步骤（RDS）评估，揭示了扩散控制的电子转移通过细胞色素。蛋白胨的电子传递速率常数（Ks）最高，为5 mg.mL - 1(1.61±0.285 s - 1)，其次是色氨酸1 mg.mL - 1（1.53±0.332 s - 1）和牛血清白蛋白1 mg.mL - 1（0.95±0.055 s - 1）。在全细胞实验中，最大输出电压分别为0.132 V（蛋白胨5 mg.mL−1）、0.117 V（色氨酸1 mg.mL−1）和0.039 V（牛血清白蛋白1 mg.mL−1），峰值功率密度分别为46.6、44.0和7.1 mW m−2。扫描电镜证实，随着氮浓度的增加，生物膜的形成增强，支持更强的电化学活性。这些结果表明氮源优化是提高酵母MFC系统中微生物电子转移和能量产量的战略途径。

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