Bioelectrochemistry最新文献_第5页

Electrochemical biosensor based on composite of gold nanoparticle/reduced-graphene oxide/graphitic carbon nitride and a caprolactone polymer for highly sensitive detection of CEA

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-12-31 DOI: 10.1016/j.bioelechem.2024.108897

Yunpeng Li, Xia Wang, Xinling Wang, Zhe Qin, Chong Li, Jing Yang, Mengmeng Cao

Carcinoembryonic antigen (CEA) is a broad-spectrum biomarker, and its accurate detection and analysis is important for early clinical diagnosis and treatment. This study aimed to develop a highly sensitive and selective sandwich-type immunosensor based on electrochemical impedance spectroscopy (EIS) for the accurate detection of CEA. A novel composite material, gold nanoparticle/reduced-graphene oxide/graphitic carbon nitride (AuNPs/rGO/g-C₃N₄), was synthesized with excellent electrical conductivity and a large specific surface area to immobilize biological probes. And ab1-CEA-ab2 formed a sandwich structure of ‘antibody-antigen-antibody’, which ensured the high selectivity of the biosensor. Furthermore, the introduction of caprolactone polymer (DMPA-PCL) significantly amplifies the impedance signal and improves the sensitivity of the analytical method. Scanning electron microscopy, x-ray diffraction, transmission electron microscopy Fourier transform infrared spectroscopy, and ultraviolet–visible spectrophotometry were used to characterise the prepared AuNPs/rGO/g-C₃N₄ and DMPA-PCL. Under the optimal conditions, the sensor showed good analytical performance for the detection of CEA with a linear range of 100 fg mL⁻¹–100 ng mL⁻¹ and a detection limit of 83.2 fg mL⁻¹. And the sandwich-type immunosensor showed good selectivity and stability for the recognition of CEA in real samples.

{"title":"Electrochemical biosensor based on composite of gold nanoparticle/reduced-graphene oxide/graphitic carbon nitride and a caprolactone polymer for highly sensitive detection of CEA","authors":"Yunpeng Li, Xia Wang, Xinling Wang, Zhe Qin, Chong Li, Jing Yang, Mengmeng Cao","doi":"10.1016/j.bioelechem.2024.108897","DOIUrl":"10.1016/j.bioelechem.2024.108897","url":null,"abstract":"<div><div>Carcinoembryonic antigen (CEA) is a broad-spectrum biomarker, and its accurate detection and analysis is important for early clinical diagnosis and treatment. This study aimed to develop a highly sensitive and selective sandwich-type immunosensor based on electrochemical impedance spectroscopy (EIS) for the accurate detection of CEA. A novel composite material, gold nanoparticle/reduced-graphene oxide/graphitic carbon nitride (AuNPs/rGO/g-C<sub>3</sub>N<sub>4</sub>), was synthesized with excellent electrical conductivity and a large specific surface area to immobilize biological probes. And ab1-CEA-ab2 formed a sandwich structure of ‘antibody-antigen-antibody’, which ensured the high selectivity of the biosensor. Furthermore, the introduction of caprolactone polymer (DMPA-PCL) significantly amplifies the impedance signal and improves the sensitivity of the analytical method. Scanning electron microscopy, x-ray diffraction, transmission electron microscopy Fourier transform infrared spectroscopy, and ultraviolet–visible spectrophotometry were used to characterise the prepared AuNPs/rGO/g-C<sub>3</sub>N<sub>4</sub> and DMPA-PCL. Under the optimal conditions, the sensor showed good analytical performance for the detection of CEA with a linear range of 100 fg mL<sup>−1</sup>–100 ng mL<sup>−1</sup> and a detection limit of 83.2 fg mL<sup>−1</sup>. And the sandwich-type immunosensor showed good selectivity and stability for the recognition of CEA in real samples.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108897"},"PeriodicalIF":4.8,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143155938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual signal amplification in ECL biosensors: A novel approach for argonaute2 detection using SAHARA CRISPR-Cas12a technology ECL生物传感器中的双信号放大：利用SAHARA CRISPR-Cas12a技术检测argonaute2的新方法

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-12-30 DOI: 10.1016/j.bioelechem.2024.108896

Yuanxun Gong , Jiayi Zhang , Zhao Lu , Jiahui Cai , Zichun Song , Jihua Wei , Chenyi Zhuo , Qianli Tang , Kai Zhang , Xianjiu Liao

Argonaute 2 (Ago2) is a crucial enzyme in the RNA interference (RNAi) pathway, essential for gene silencing via the cleavage of target messenger RNA (mRNA) mediated by microRNA (miRNA) or small interfering RNA (siRNA). The activity of Ago2 is a significant biomarker for various diseases, including cancer and viral infections, necessitating precise monitoring techniques. Traditional methods for detecting Ago2 activity are often cumbersome and lack the necessary sensitivity and specificity for low-abundance targets in complex samples. This study presents an innovative biosensor utilizing electrochemiluminescence (ECL) technology combined with the SAHARA (Split Activator for Highly Accessible RNA Analysis) CRISPR-Cas12a system to detect Ago2 activity with high sensitivity and specificity. The introduction of Blocker RNA in the activation mechanism enhances the specificity of CRISPR-Cas12a, ensuring accurate signal generation. The dual signal amplification strategy, combining RISC-assisted and CRISPR-Cas12a-mediated cleavage, enhances the biosensor’s sensitivity. The developed ECL biosensor demonstrated a remarkable limit of detection (LOD) of 0.145 aM, along with excellent precision, stability, and specificity. These attributes make it a powerful tool for detecting Ago2 activity in clinical diagnostics and research settings.

Argonaute 2 （Ago2）是RNA干扰（RNAi）途径中的一种重要酶，通过microRNA （miRNA）或小干扰RNA （siRNA）介导的靶信使RNA （mRNA）的裂解实现基因沉默。Ago2的活性是多种疾病的重要生物标志物，包括癌症和病毒感染，需要精确的监测技术。传统的检测Ago2活性的方法通常是繁琐的，并且缺乏对复杂样品中低丰度目标的必要灵敏度和特异性。本研究提出了一种创新的生物传感器，利用电化学发光（ECL）技术结合SAHARA (Split Activator for Highly Accessible RNA Analysis) CRISPR-Cas12a系统，以高灵敏度和特异性检测Ago2活性。在激活机制中引入阻断RNA，增强了CRISPR-Cas12a的特异性，保证了准确的信号生成。双信号扩增策略，结合risc辅助和crispr - cas12a介导的切割，提高了生物传感器的灵敏度。所开发的ECL生物传感器的检测限（LOD）为0.145 aM，具有良好的精度、稳定性和特异性。这些特性使其成为临床诊断和研究设置中检测Ago2活性的强大工具。

{"title":"Dual signal amplification in ECL biosensors: A novel approach for argonaute2 detection using SAHARA CRISPR-Cas12a technology","authors":"Yuanxun Gong , Jiayi Zhang , Zhao Lu , Jiahui Cai , Zichun Song , Jihua Wei , Chenyi Zhuo , Qianli Tang , Kai Zhang , Xianjiu Liao","doi":"10.1016/j.bioelechem.2024.108896","DOIUrl":"10.1016/j.bioelechem.2024.108896","url":null,"abstract":"<div><div>Argonaute 2 (Ago2) is a crucial enzyme in the RNA interference (RNAi) pathway, essential for gene silencing via the cleavage of target messenger RNA (mRNA) mediated by microRNA (miRNA) or small interfering RNA (siRNA). The activity of Ago2 is a significant biomarker for various diseases, including cancer and viral infections, necessitating precise monitoring techniques. Traditional methods for detecting Ago2 activity are often cumbersome and lack the necessary sensitivity and specificity for low-abundance targets in complex samples. This study presents an innovative biosensor utilizing electrochemiluminescence (ECL) technology combined with the SAHARA (Split Activator for Highly Accessible RNA Analysis) CRISPR-Cas12a system to detect Ago2 activity with high sensitivity and specificity. The introduction of Blocker RNA in the activation mechanism enhances the specificity of CRISPR-Cas12a, ensuring accurate signal generation. The dual signal amplification strategy, combining RISC-assisted and CRISPR-Cas12a-mediated cleavage, enhances the biosensor’s sensitivity. The developed ECL biosensor demonstrated a remarkable limit of detection (LOD) of 0.145 aM, along with excellent precision, stability, and specificity. These attributes make it a powerful tool for detecting Ago2 activity in clinical diagnostics and research settings.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108896"},"PeriodicalIF":4.8,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aptamer-based DNAzyme walker electrochemical biosensing strategy for Acinetobacter baumannii detection 基于核酸适配体的DNAzyme walker电化学生物传感技术检测鲍曼不动杆菌。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-12-28 DOI: 10.1016/j.bioelechem.2024.108895

Linhong Cao , Tianyu Wang , Jingling Xie , Yihua Wang , Yaxin Huang , Sijian Luo , Xiaoting Zhan , Hui Jiang , Liuxin Ran , Xing Jin , Jinbo Liu , Baolin Li

In this study, an innovative electrochemical biosensor was developed for the rapid, specific, and sensitive detection of Acinetobacter baumannii without the need for sample pretreatment. The biosensor utilized an aptamer as a specific capture probe for A. baumannii and employed a self-powered DNAzyme walker cleavage cycle reaction to achieve signal amplification. Upon introduction of the target bacteria, the aptamer captured the bacteria and released the Trigger, activating the DNAzyme to cleave the substrate chain containing methylene blue (MB). This led to the release of MB-labeled DNA fragments from the electrode surface, resulting in a significant decrease in the square wave voltammetry (SWV) signal of MB on the sensing platform. The limit of detection (LOD) for A. baumannii was determined to be 30 CFU/mL, enabling discrimination of the target bacteria from other common clinical isolates. Furthermore, the biosensor’s potential for real sample analysis was demonstrated in cerebrospinal fluid (CSF), showcasing its efficacy and versatility as a biosensing tool with wide-ranging applications in disease diagnosis and bioanalysis.

本研究开发了一种新型电化学生物传感器，可快速、特异、灵敏地检测鲍曼不动杆菌，而无需对样品进行预处理。该生物传感器利用适体作为鲍曼不动杆菌的特异性捕获探针，采用自供电的DNAzyme walker裂解循环反应实现信号放大。在引入目标细菌后，适体捕获细菌并释放触发器，激活DNAzyme裂解含有亚甲基蓝（MB）的底物链。这导致MB标记的DNA片段从电极表面释放，导致传感平台上MB的方波伏安（SWV）信号显著降低。鲍曼不动杆菌的检出限（LOD）为30 CFU/mL，可与其他常见临床分离菌区分。此外，该生物传感器在脑脊液（CSF）中进行真实样本分析的潜力得到了证明，展示了其作为一种生物传感工具的有效性和多功能性，在疾病诊断和生物分析中有着广泛的应用。

{"title":"Aptamer-based DNAzyme walker electrochemical biosensing strategy for Acinetobacter baumannii detection","authors":"Linhong Cao , Tianyu Wang , Jingling Xie , Yihua Wang , Yaxin Huang , Sijian Luo , Xiaoting Zhan , Hui Jiang , Liuxin Ran , Xing Jin , Jinbo Liu , Baolin Li","doi":"10.1016/j.bioelechem.2024.108895","DOIUrl":"10.1016/j.bioelechem.2024.108895","url":null,"abstract":"<div><div>In this study, an innovative electrochemical biosensor was developed for the rapid, specific, and sensitive detection of <em>Acinetobacter baumannii</em> without the need for sample pretreatment. The biosensor utilized an aptamer as a specific capture probe for <em>A. baumannii</em> and employed a self-powered DNAzyme walker cleavage cycle reaction to achieve signal amplification. Upon introduction of the target bacteria, the aptamer captured the bacteria and released the Trigger, activating the DNAzyme to cleave the substrate chain containing methylene blue (MB). This led to the release of MB-labeled DNA fragments from the electrode surface, resulting in a significant decrease in the square wave voltammetry (SWV) signal of MB on the sensing platform. The limit of detection (LOD) for <em>A. baumannii</em> was determined to be 30 CFU/mL, enabling discrimination of the target bacteria from other common clinical isolates. Furthermore, the biosensor’s potential for real sample analysis was demonstrated in cerebrospinal fluid (CSF), showcasing its efficacy and versatility as a biosensing tool with wide-ranging applications in disease diagnosis and bioanalysis.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108895"},"PeriodicalIF":4.8,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leveraging self-signal amplifying poly(acrylic acid)/polyaniline electrodes for label-free electrochemical immunoassays in protein biomarker detection 利用自信号放大聚（丙烯酸）/聚苯胺电极在蛋白质生物标志物检测中的无标记电化学免疫分析。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-12-28 DOI: 10.1016/j.bioelechem.2024.108894

Supakeit Chanarsa , Sopit Phetsang , Wiradej Thongsuwan , Thunwadee Limtharakul , Jidapha Tinoi , Jaroon Jakmunee , Kontad Ounnunkad

Accurate quantification of specific biomarkers is essential for clinical diagnosis and evaluating therapeutic efficacy. A self-signal-amplifying poly(acrylic acid) (PAA)/polyaniline (PANI) film-modified disposable and cost-effective screen-printed carbon electrode (SPCE) has been developed for constructing new label-free immunosensors targeting two model biomarkers: human immunoglobulin G (IgG) and alpha-fetoprotein (AFP). The electrochemically deposited PAA/PANI film on the SPCE serves a dual function: both a bio-immobilization support and a signal amplifier, enhancing biomarker detection sensitivity and efficiency. The self-signal amplification properties of PANI streamline the detection process. At the same time, the high-density surface carboxyl groups from embedded PAA enable covalent conjugation with capture antibodies (anti-IgG and anti-AFP). Subsequently, antibody-immobilized PAA/PANI film-modified SPCEs, as immunosensors, successfully detect IgG and AFP without the need for external redox probes. The reductions in the electrochemical PANI signals of the immunosensors are linearly proportional to the logarithm of IgG and AFP concentrations. The proposed immunosensors exhibit sufficiently wide ranges of calibration curves from 0.10 to 50 ng mL⁻¹, with limits of detection of 0.080 ng mL⁻¹ for IgG and 0.090 ng mL⁻¹ for AFP. The sensors exhibit satisfactory sensitivity and selectivity, indicating their potential for accurate and reliable detection.

特异性生物标志物的准确定量对临床诊断和评估治疗效果至关重要。制备了一种自信号放大的聚丙烯酸(PAA)/聚苯胺（PANI）薄膜修饰的一次性丝网印刷碳电极（SPCE），用于构建新的无标记免疫传感器，该电极针对两种模型生物标志物：人免疫球蛋白G （IgG）和甲胎蛋白（AFP）。电化学沉积在SPCE上的PAA/PANI膜具有双重功能：生物固定支架和信号放大器，提高了生物标志物检测的灵敏度和效率。聚苯胺的自信号放大特性简化了检测过程。同时，包埋PAA的高密度表面羧基使其能够与捕获抗体（抗igg和抗afp）共价偶联。随后，固定化PAA/PANI膜修饰的spce作为免疫传感器，成功检测IgG和AFP，而无需外部氧化还原探针。免疫传感器电化学PANI信号的减少与IgG和AFP浓度的对数成线性比例。所提出的免疫传感器具有足够宽的校准曲线范围，从0.10到50 ng mL-1， IgG的检测限为0.080 ng mL-1， AFP的检测限为0.090 ng mL-1。传感器表现出令人满意的灵敏度和选择性，表明它们具有准确可靠检测的潜力。

{"title":"Leveraging self-signal amplifying poly(acrylic acid)/polyaniline electrodes for label-free electrochemical immunoassays in protein biomarker detection","authors":"Supakeit Chanarsa , Sopit Phetsang , Wiradej Thongsuwan , Thunwadee Limtharakul , Jidapha Tinoi , Jaroon Jakmunee , Kontad Ounnunkad","doi":"10.1016/j.bioelechem.2024.108894","DOIUrl":"10.1016/j.bioelechem.2024.108894","url":null,"abstract":"<div><div>Accurate quantification of specific biomarkers is essential for clinical diagnosis and evaluating therapeutic efficacy. A self-signal-amplifying poly(acrylic acid) (PAA)/polyaniline (PANI) film-modified disposable and cost-effective screen-printed carbon electrode (SPCE) has been developed for constructing new label-free immunosensors targeting two model biomarkers: human immunoglobulin G (IgG) and alpha-fetoprotein (AFP). The electrochemically deposited PAA/PANI film on the SPCE serves a dual function: both a bio-immobilization support and a signal amplifier, enhancing biomarker detection sensitivity and efficiency. The self-signal amplification properties of PANI streamline the detection process. At the same time, the high-density surface carboxyl groups from embedded PAA enable covalent conjugation with capture antibodies (anti-IgG and anti-AFP). Subsequently, antibody-immobilized PAA/PANI film-modified SPCEs, as immunosensors, successfully detect IgG and AFP without the need for external redox probes. The reductions in the electrochemical PANI signals of the immunosensors are linearly proportional to the logarithm of IgG and AFP concentrations. The proposed immunosensors exhibit sufficiently wide ranges of calibration curves from 0.10 to 50 ng mL<sup>−1</sup>, with limits of detection of 0.080 ng mL<sup>−1</sup> for IgG and 0.090 ng mL<sup>−1</sup> for AFP. The sensors exhibit satisfactory sensitivity and selectivity, indicating their potential for accurate and reliable detection.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108894"},"PeriodicalIF":4.8,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrocultivation of Arabidopsis thaliana increases water and mineral absorption, electric charge and auxin accumulation, enhancing growth and development 电栽培增加拟南芥对水分和矿物质的吸收、电荷和生长素的积累，促进生长发育。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-12-27 DOI: 10.1016/j.bioelechem.2024.108893

Sora Lee , Myung-Min Oh

Numerous studies in various species have demonstrated that the application of an electric field can improve plant growth. However, plants showed inconsistent responses and the background mechanism for responses to electric fields remain unclear. Here, to deepen our understanding of the mechanisms involved in electric field–induced changes in physiology, we investigated the effects of electric fields on the growth and development of Arabidopsis (Arabidopsis thaliana). To this end, we cultivated Arabidopsis plants under 5 kV/m electric fields oriented vertically or horizontally. Regardless of the electric field direction, the exposed plants had significantly more leaves and greater biomass compared to the control group, which was not exposed to an additional electric field. Exposure to the electric fields also accelerated flowering. Auxin concentration in Arabidopsis leaves exhibited significant increase following exposure to electric field, supporting the enhanced shoot growth. Moreover, well-developed xylem and phloem under vertical electric fields facilitated increased absorption of water and nutrients. Gene ontology annotation and KEGG pathway analysis identified numerous electric field–responsive genes. Overall, this study elucidates mechanisms of the plant response to electric fields and represents a step towards developing technologies that enhance crop productivity.

对不同物种的大量研究表明，施加电场可以促进植物生长。然而，植物表现出不一致的反应，电场反应的背景机制尚不清楚。为了进一步了解电场诱导生理变化的机制，我们研究了电场对拟南芥（Arabidopsis thaliana）生长发育的影响。为此，我们在5 kV/m的垂直或水平电场下培养拟南芥植株。无论电场的方向如何，与没有暴露于额外电场的对照组相比，暴露的植物有更多的叶子和更大的生物量。暴露在电场中也加速了开花。电场处理后，拟南芥叶片中生长素浓度显著升高，支持了叶片生长的增强。此外，在垂直电场作用下，木质部和韧皮部发育良好，有利于增加水分和养分的吸收。基因本体注释和KEGG通路分析鉴定出大量电场响应基因。总的来说，这项研究阐明了植物对电场的反应机制，并代表着朝着开发提高作物生产力的技术迈出了一步。

{"title":"Electrocultivation of Arabidopsis thaliana increases water and mineral absorption, electric charge and auxin accumulation, enhancing growth and development","authors":"Sora Lee , Myung-Min Oh","doi":"10.1016/j.bioelechem.2024.108893","DOIUrl":"10.1016/j.bioelechem.2024.108893","url":null,"abstract":"<div><div>Numerous studies in various species have demonstrated that the application of an electric field can improve plant growth. However, plants showed inconsistent responses and the background mechanism for responses to electric fields remain unclear. Here, to deepen our understanding of the mechanisms involved in electric field–induced changes in physiology, we investigated the effects of electric fields on the growth and development of Arabidopsis (<em>Arabidopsis thaliana</em>). To this end, we cultivated Arabidopsis plants under 5 kV/m electric fields oriented vertically or horizontally. Regardless of the electric field direction, the exposed plants had significantly more leaves and greater biomass compared to the control group, which was not exposed to an additional electric field. Exposure to the electric fields also accelerated flowering. Auxin concentration in Arabidopsis leaves exhibited significant increase following exposure to electric field, supporting the enhanced shoot growth. Moreover, well-developed xylem and phloem under vertical electric fields facilitated increased absorption of water and nutrients. Gene ontology annotation and KEGG pathway analysis identified numerous electric field–responsive genes. Overall, this study elucidates mechanisms of the plant response to electric fields and represents a step towards developing technologies that enhance crop productivity.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108893"},"PeriodicalIF":4.8,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low background catalytic redox recycling coupled with hybridization chain reaction amplification for highly sensitive electrochemical aptamer luteinizing hormone assay 低背景催化氧化还原循环耦合杂交链反应扩增高灵敏度电化学适体黄体生成素测定。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-12-26 DOI: 10.1016/j.bioelechem.2024.108888

Yuan Ming , Yujie Liu , Daxiu Li , Bingying Jiang , Yun Xiang , Ruo Yuan

The concentration variation of luteinizing hormone (LH) regulates the cell cycle of oocyte meiosis and significantly affect the whole reproductive cycle. Sensitively quantifying the LH biomarker therefore plays an important role for reproductive disease diagnosis. By coupling a new low background catalytic redox recycling strategy with hybridization chain reaction (HCR), we propose a highly sensitive bio-electrochemical aptamer LH sensing method. LH analyte molecules bind aptamer strands in duplex DNAs to liberate ssDNAs, which trigger HCR generation of [Ru(NH₃)₆]Cl₃ (RuHex)-modified dsDNA polymers on sensor electrode. Subsequent electrochemical redox recycling of RuHex mediated by K₃[Fe(CN)₆] thus exhibits greatly magnified currents for ultrasensitive LH assay. The synergistic integration of HCR signal amplification with low background redox recycling leads to highly enhanced signal-to-noise ratio and sensitivity for detecting LH down to 6.03 pM. In addition, LH sensing in diluted human serums has been tested and verified, making such sensor a robust detection platform for monitoring diverse biomarkers at low levels for early diagnosing diseases.

黄体生成素（LH）的浓度变化调节卵母细胞减数分裂的细胞周期，显著影响整个生殖周期。因此，对LH生物标志物的敏感量化对生殖疾病的诊断具有重要作用。通过将低背景催化氧化还原回收策略与杂化链反应（HCR）相结合，提出了一种高灵敏度的生物电化学适体LH检测方法。LH分析物分子结合双链dna的适体链释放ssdna，触发传感器电极上的[Ru(NH3)6]Cl3 （RuHex）修饰的dsDNA聚合物HCR生成。随后由K3[Fe(CN)6]介导的RuHex的电化学氧化还原循环因此显示出超灵敏LH测定的极大放大电流。HCR信号放大与低背景氧化还原循环的协同集成，使检测LH的信噪比和灵敏度提高到6.03 pM。此外，在稀释的人血清中检测LH已经得到了测试和验证，使这种传感器成为一个强大的检测平台，可以监测低水平的多种生物标志物，用于疾病的早期诊断。

{"title":"Low background catalytic redox recycling coupled with hybridization chain reaction amplification for highly sensitive electrochemical aptamer luteinizing hormone assay","authors":"Yuan Ming , Yujie Liu , Daxiu Li , Bingying Jiang , Yun Xiang , Ruo Yuan","doi":"10.1016/j.bioelechem.2024.108888","DOIUrl":"10.1016/j.bioelechem.2024.108888","url":null,"abstract":"<div><div>The concentration variation of luteinizing hormone (LH) regulates the cell cycle of oocyte meiosis and significantly affect the whole reproductive cycle. Sensitively quantifying the LH biomarker therefore plays an important role for reproductive disease diagnosis. By coupling a new low background catalytic redox recycling strategy with hybridization chain reaction (HCR), we propose a highly sensitive bio-electrochemical aptamer LH sensing method. LH analyte molecules bind aptamer strands in duplex DNAs to liberate ssDNAs, which trigger HCR generation of [Ru(NH<sub>3</sub>)<sub>6</sub>]Cl<sub>3</sub> (RuHex)-modified dsDNA polymers on sensor electrode. Subsequent electrochemical redox recycling of RuHex mediated by K<sub>3</sub>[Fe(CN)<sub>6</sub>] thus exhibits greatly magnified currents for ultrasensitive LH assay. The synergistic integration of HCR signal amplification with low background redox recycling leads to highly enhanced signal-to-noise ratio and sensitivity for detecting LH down to 6.03 pM. In addition, LH sensing in diluted human serums has been tested and verified, making such sensor a robust detection platform for monitoring diverse biomarkers at low levels for early diagnosing diseases.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108888"},"PeriodicalIF":4.8,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A label-free electrochemical biosensor for sensitive analysis of the PARP-1 activity 用于PARP-1活性敏感分析的无标记电化学生物传感器。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-12-26 DOI: 10.1016/j.bioelechem.2024.108891

Haitang Yang , Siming Pu , Penghua Shu , Jiapan Wang , YuYu Chen , Xinshuo Yang , Yuce Hou , Wei Wei

Early diagnosis of tumors is becoming increasingly important in modern healthcare. As studies have demonstrated, Poly(ADP)ribose polymerase-1 (PARP-1) is overexpressed in more aggressive tumors. Consequently, sensitive detection of PARP-1 activity holds significant practical importance in clinical diagnostics and biomedical research. Herein, an electrochemical biosensor for the sensitive monitoring of the PARP-1 activity have been proposed. The presence of target PARP-1 firstly triggers enzyme-initiated auto-PARylation and formed negatively charged polymer consisting of a few to 200 ADP-ribose units. Due to electrostatic adsorption, negatively charged PAR will bind with a large number of positively charged methylene blue (MB) electroactive molecules. By detecting the electrochemical signal of MB on the indium tin oxide (ITO) electrode, PARP-1 activity detection was achieved with a linear detection range of 0–1.0 U and a detection limit as low as 0.003 U. The proposed biosensor shows great prospects of clinical application.

肿瘤的早期诊断在现代医疗保健中变得越来越重要。研究表明，聚（ADP）核糖聚合酶-1 （PARP-1）在侵袭性更强的肿瘤中过表达。因此，PARP-1活性的灵敏检测在临床诊断和生物医学研究中具有重要的实际意义。本文提出了一种用于灵敏监测PARP-1活性的电化学生物传感器。目标PARP-1的存在首先触发酶启动的自parp化，形成由几个到200个adp核糖单位组成的带负电荷的聚合物。由于静电吸附作用，带负电的PAR会与大量带正电的亚甲基蓝（MB）电活性分子结合。通过在氧化铟锡（ITO）电极上检测MB的电化学信号，实现了PARP-1活性的检测，线性检测范围为0-1.0 U，检出限低至0.003 U，该生物传感器具有广阔的临床应用前景。

{"title":"A label-free electrochemical biosensor for sensitive analysis of the PARP-1 activity","authors":"Haitang Yang , Siming Pu , Penghua Shu , Jiapan Wang , YuYu Chen , Xinshuo Yang , Yuce Hou , Wei Wei","doi":"10.1016/j.bioelechem.2024.108891","DOIUrl":"10.1016/j.bioelechem.2024.108891","url":null,"abstract":"<div><div>Early diagnosis of tumors is becoming increasingly important in modern healthcare. <u>As studies have demonstrated, Poly(ADP)ribose polymerase-1 (PARP-1) is overexpressed in more aggressive tumors. Consequently, sensitive detection of PARP-1 activity holds significant practical importance in clinical diagnostics and biomedical research</u>. Herein, an electrochemical biosensor for the sensitive monitoring of the PARP-1 activity have been proposed. The presence of target PARP-1 firstly triggers enzyme-initiated auto-PARylation and formed negatively charged polymer consisting of a few to 200 ADP-ribose units. Due to electrostatic adsorption, negatively charged PAR will bind with a large number of positively charged methylene blue (MB) electroactive molecules. By detecting the electrochemical signal of MB on the <u>indium tin oxide (ITO)</u> electrode, PARP-1 activity detection was achieved with a linear detection range of 0–1.0 U and a detection limit as low as 0.003 U. The proposed biosensor shows great prospects of clinical application.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108891"},"PeriodicalIF":4.8,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sulfate reducing bacteria corrosion of a 90/10 Cu-Ni alloy coupled to an Al sacrificial anode 90/10 Cu-Ni合金与Al牺牲阳极的硫酸盐还原细菌腐蚀。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-12-26 DOI: 10.1016/j.bioelechem.2024.108892

Huixuan Qian , Tianguan Wang , Peng Xu , Zhiyuan Feng , Bing Lei , Ping Zhang , Honglei Guo , Guozhe Meng

This study investigates the corrosion of 90/10 copper-nickel (Cu-Ni) alloy caused by sulfate-reducing bacteria (SRB) in the presence of aluminum anodes, with particular emphasis on the role of electron supply in microbial corrosion and the resulting local corrosion failures. The study reveals that the electron supply from the anode supports SRB growth on the Cu-Ni alloy through an “Electrons-siphoning” mechanism. However, the supply is insufficient to sustain the SRB population, resulting in ineffective cathodic protection (i_corr = 2.34 × 10⁻⁶ A cm⁻²). The addition of 20 ppm riboflavin (RF) to the SRB biofilm enhances electrical activity and increases the electron donor density, thereby restoring the anode’s protective effect. As a result, the i_corr of the 90/10 Cu-Ni alloy decreases by an order of magnitude (to 3.5 × 10⁻⁷ A cm⁻²). These findings provide valuable new insights into the mechanisms of microbial corrosion.

本研究研究了在铝阳极存在的情况下，硫酸盐还原菌（SRB）对90/10铜镍（Cu-Ni）合金的腐蚀，特别强调了电子供应在微生物腐蚀中的作用以及由此导致的局部腐蚀失效。研究表明，阳极的电子供给通过“电子虹吸”机制支持SRB在Cu-Ni合金上的生长。然而，供应不足以维持SRB种群，导致无效的阴极保护（icorr = 2.34 × 10-6 A cm-2）。在SRB生物膜中加入20ppm的核黄素（RF）可以增强电活性，增加电子供体密度，从而恢复阳极的保护作用。因此，90/10 Cu-Ni合金的icorr降低了一个数量级（为3.5 × 10-7 a cm-2）。这些发现为微生物腐蚀的机理提供了有价值的新见解。

{"title":"Sulfate reducing bacteria corrosion of a 90/10 Cu-Ni alloy coupled to an Al sacrificial anode","authors":"Huixuan Qian , Tianguan Wang , Peng Xu , Zhiyuan Feng , Bing Lei , Ping Zhang , Honglei Guo , Guozhe Meng","doi":"10.1016/j.bioelechem.2024.108892","DOIUrl":"10.1016/j.bioelechem.2024.108892","url":null,"abstract":"<div><div>This study investigates the corrosion of 90/10 copper-nickel (Cu-Ni) alloy caused by sulfate-reducing bacteria (SRB) in the presence of aluminum anodes, with particular emphasis on the role of electron supply in microbial corrosion and the resulting local corrosion failures. The study reveals that the electron supply from the anode supports SRB growth on the Cu-Ni alloy through an “Electrons-siphoning” mechanism. However, the supply is insufficient to sustain the SRB population, resulting in ineffective cathodic protection (<em>i</em><sub>corr</sub> = 2.34 × 10<sup>−6</sup> A cm<sup>−2</sup>). The addition of 20 ppm riboflavin (RF) to the SRB biofilm enhances electrical activity and increases the electron donor density, thereby restoring the anode’s protective effect. As a result, the <em>i</em><sub>corr</sub> of the 90/10 Cu-Ni alloy decreases by an order of magnitude (to 3.5 × 10<sup>−7</sup> A cm<sup>−2</sup>). These findings provide valuable new insights into the mechanisms of microbial corrosion.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108892"},"PeriodicalIF":4.8,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient nitrate removal via microorganism-iron oxide co-evolution on biocathode surface 生物阴极表面微生物-氧化铁协同进化高效去除硝酸盐。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-12-25 DOI: 10.1016/j.bioelechem.2024.108889

Xiaojun Liu , Huihui Dong , Qinyu Wang , Jing Yang , Xinru Zhai , Mingzhen Lin , Kaixin Liu , Qinzheng Yang

Sediment microbial fuel cell (SMFC) is a device for biological denitrification, in which electrons produced by sediment microorganisms can be transferred to the upper layer of the water column lacking electron donors. However, the low efficiency of denitrifying bacteria in acquiring electrons and enriching at the cathode greatly hinders the application of SMFC for nitrogen removal. In this study, we report a novel method of constructing a high-performance biocathode by modifying electrodes with zero-valent iron to enhance the enrichment and electron transfer of electroactive bacteria. The surface chemical and biological analysis of the biocathode revealed that the ZVI gradually oxidized to form magnetite and goethite, and finally stabilized into better crystallized lepidocrocite. On the other hand, the microbial community of the biocathode gradually evolved into a community dominated by denitrifying bacteria, specifically Clostridium. The co-evolved “Clostridium-lepidocrocite” composite endows the sediment microbial fuel cell with a 99% nitrate removal capacity. These results indicate that the cathode constructed by using ZVI modified electrode achieves efficient nitrate reduction by denitrifying bacteria. Furthermore, the construction method of biocathode may also have the potential application in water remediation and the geochemical cycling of elements.

沉积物微生物燃料电池（SMFC）是一种生物反硝化装置，其中沉积物微生物产生的电子可以转移到没有电子供体的水柱上层。然而，反硝化细菌在阴极获取电子和富集的效率较低，极大地阻碍了SMFC在脱氮中的应用。在这项研究中，我们报告了一种通过用零价铁修饰电极来构建高性能生物阴极的新方法，以增强电活性细菌的富集和电子转移。生物阴极的表面化学和生物学分析表明，ZVI逐渐氧化形成磁铁矿和针铁矿，最终稳定为结晶较好的鳞状云母。另一方面，生物阴极的微生物群落逐渐演变为以反硝化细菌为主的群落，特别是梭状芽胞杆菌。共同进化的“梭状芽孢杆菌”复合材料赋予沉积物微生物燃料电池99%的硝酸盐去除能力。这些结果表明，用ZVI修饰电极构建的阴极可以实现反硝化细菌对硝酸盐的高效还原。此外，生物阴极的构建方法在水体修复和元素的地球化学循环中也有潜在的应用前景。

{"title":"Efficient nitrate removal via microorganism-iron oxide co-evolution on biocathode surface","authors":"Xiaojun Liu , Huihui Dong , Qinyu Wang , Jing Yang , Xinru Zhai , Mingzhen Lin , Kaixin Liu , Qinzheng Yang","doi":"10.1016/j.bioelechem.2024.108889","DOIUrl":"10.1016/j.bioelechem.2024.108889","url":null,"abstract":"<div><div>Sediment microbial fuel cell (SMFC) is a device for biological denitrification, in which electrons produced by sediment microorganisms can be transferred to the upper layer of the water column lacking electron donors. However, the low efficiency of denitrifying bacteria in acquiring electrons and enriching at the cathode greatly hinders the application of SMFC for nitrogen removal. In this study, we report a novel method of constructing a high-performance biocathode by modifying electrodes with zero-valent iron to enhance the enrichment and electron transfer of electroactive bacteria. The surface chemical and biological analysis of the biocathode revealed that the ZVI gradually oxidized to form magnetite and goethite, and finally stabilized into better crystallized lepidocrocite. On the other hand, the microbial community of the biocathode gradually evolved into a community dominated by denitrifying bacteria, specifically <em>Clostridium</em>. The co-evolved “<em>Clostridium</em>-lepidocrocite” composite endows the sediment microbial fuel cell with a 99% nitrate removal capacity. These results indicate that the cathode constructed by using ZVI modified electrode achieves efficient nitrate reduction by denitrifying bacteria. Furthermore, the construction method of biocathode may also have the potential application in water remediation and the geochemical cycling of elements.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108889"},"PeriodicalIF":4.8,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrical characteristics extraction and analysis method for the membrane of medaka embryo during its development using electrical impedance spectroscopy 建立了一种利用电阻抗谱法提取和分析藻胚发育过程中膜电特性的方法。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-12-24 DOI: 10.1016/j.bioelechem.2024.108885

Lu Yang , Yang Wu , Songpei Hu , Jiafeng Yao , Fangming Chen

An electrical characteristics extraction and analysis method for membrane of medaka embryo during its development using Electrical Impedance Spectroscopy (EIS) is proposed. The proposed method is non-invasive, it doesn’t affect the embryo’s development. Embryo’s equivalent electrical circuit (EEC) model is established to extract membrane’s electrical characteristics, it is used to fit the embryo’s electrical impedance curves in different developmental stages. The equivalent resistances and capacitances of membrane are regarded as the electrical characteristics to analyze the membrane’s physiological changes during embryo’s development. To achieve the embryo’s electrical impedance curve from the measurement system exactly, an EIT-assisted electrical impedance curve extraction method is innovatively introduced. The extracted electrical characteristics of the membrane reflect its physiological changes well in both simulation and experiment. In experiment, the equivalent capacitances of embryo’s membrane are increasing, while the equivalent resistances show a downward trend from neurula stage to hatching stage. The experiment results indicate that the permeability of membrane becomes higher and the thickness of membrane becomes thinner from neurula stage to hatching stage.

提出了一种利用电阻抗谱（EIS）提取和分析medaka胚胎发育过程中膜电特性的方法。该方法是非侵入性的，不影响胚胎的发育。建立胚胎等效电路（EEC）模型提取膜的电特性，用于拟合胚胎在不同发育阶段的电阻抗曲线。以膜的等效电阻和等效电容作为电特性，分析胚胎发育过程中膜的生理变化。为了准确地从测量系统中获得胚胎的电阻抗曲线，创新性地介绍了一种eit辅助的电阻抗曲线提取方法。提取的膜电特性在模拟和实验中都很好地反映了膜的生理变化。在实验中，胚膜的等效电容逐渐增大，而从神经胚期到孵化期，等效电阻呈下降趋势。实验结果表明，从神经胚期到孵化期，膜的通透性变高，膜的厚度变薄。

{"title":"An electrical characteristics extraction and analysis method for the membrane of medaka embryo during its development using electrical impedance spectroscopy","authors":"Lu Yang , Yang Wu , Songpei Hu , Jiafeng Yao , Fangming Chen","doi":"10.1016/j.bioelechem.2024.108885","DOIUrl":"10.1016/j.bioelechem.2024.108885","url":null,"abstract":"<div><div>An electrical characteristics extraction and analysis method for membrane of medaka embryo during its development using Electrical Impedance Spectroscopy (EIS) is proposed. The proposed method is non-invasive, it doesn’t affect the embryo’s development. Embryo’s equivalent electrical circuit (EEC) model is established to extract membrane’s electrical characteristics, it is used to fit the embryo’s electrical impedance curves in different developmental stages. The equivalent resistances and capacitances of membrane are regarded as the electrical characteristics to analyze the membrane’s physiological changes during embryo’s development. To achieve the embryo’s electrical impedance curve from the measurement system exactly, an EIT-assisted electrical impedance curve extraction method is innovatively introduced. The extracted electrical characteristics of the membrane reflect its physiological changes well in both simulation and experiment. In experiment, the equivalent capacitances of embryo’s membrane are increasing, while the equivalent resistances show a downward trend from neurula stage to hatching stage. The experiment results indicate that the permeability of membrane becomes higher and the thickness of membrane becomes thinner from neurula stage to hatching stage.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108885"},"PeriodicalIF":4.8,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0