Bioelectrochemistry最新文献

英文中文

Emerging screening platform characterises aminoquinoline structure–activity relationships with phospholipid layers

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-02-01 DOI: 10.1016/j.bioelechem.2025.108927

Bethany Crow , Roland Grafstrom , Vesa Hongisto , Mitali Kamat , Nikil Kapur , Ross Kelly , Josh Owen , Ashi Rashid , William Stokes , Nicola William , Jeanine Williams , Andrew Nelson

Aminoquinolines (AQ) and substituted aminoquinolines (s-AQ) interact with electrochemically monitored supported dioleoyl phosphatidylcholine (DOPC) monolayers and immobilised artificial membranes (IAM) on HPLC column. The electrochemical sensor records adsorption/partition of the compound on and into the layer as well as specific interactions due to the location of the compound in the layer. HPLC-IAM technology measures the partition coefficient between the solution and phospholipid including partition due to interaction of the positive molecular charge with the phospholipid polar heads. The monolayer interaction results were combined and normalised for the neutral compounds’ lipophilicity as a log biomembrane affinity index (‘log BAI’) to exemplify charge and structural features in the interaction. A ChimeraX molecular modelling procedure was used to aid in the results interpretation. A compound ToxScore value was derived from 5 in vitro assays. The ‘log BAI’ exhibited a linear relationship with the AQ pK_a values showing that the interaction was related to the molecular positive charge and to the electron donating properties of the –NH₂ group. The correlation outliers showed a tendency/no tendency to H-bonding with the polar groups and a superficial/deeper location respectively in the phospholipid layer. The s-AQ ‘log BAI’ value displayed a power correlation with the compounds’ ToxScore values.

氨基喹啉（AQ）和取代氨基喹啉（s-AQ）与高效液相色谱柱上的电化学监测支撑二油酰磷脂酰胆碱（DOPC）单层和固定人工膜（IAM）相互作用。电化学传感器记录化合物在层上和层中的吸附/分离情况，以及化合物在层中的位置所导致的特定相互作用。HPLC-IAM 技术测量溶液与磷脂之间的分配系数，包括正分子电荷与磷脂极性头相互作用产生的分配。结合单层相互作用结果，并根据中性化合物的亲脂性将其归一化为生物膜亲和指数对数（"log BAI"），以体现相互作用中的电荷和结构特征。ChimeraX 分子建模程序用于帮助解释结果。从 5 项体外检测中得出了化合物 ToxScore 值。对数 BAI "与 AQ pKa 值呈线性关系，表明相互作用与分子正电荷和 -NH2 基团的电子捐赠特性有关。相关离群值分别显示了与极性基团的 H 键作用倾向/无倾向，以及在磷脂层中的浅层/深层位置。s-AQ 的 "对数 BAI "值与化合物的 ToxScore 值呈幂相关性。

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引用次数: 0

Corrigendum to “Analysis of electromagnetic response of cells and lipid membranes using a model-free method” [Bioelectrochemistry 152 (2023) 108444] 对 "利用无模型方法分析细胞和脂膜的电磁响应"[生物电化学 152 (2023) 108444] 的更正。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-02-01 DOI: 10.1016/j.bioelechem.2024.108841

Yingxian Lu , Xiaping Tang , Yanyu Zhao , Tianyu Jiang , Jiayao Zhou , Xiaofei Wang , Bing Huang , Lingyu Liu , Hu Deng , Yujing Huang , Yigong Shi

引用次数: 0

CeO2@Co3O4-decorated carbon felt anode with mesoporous nanoparticles for enhanced POM wastewater treatment and electricity generation in microbial fuel cells

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-01-31 DOI: 10.1016/j.bioelechem.2025.108924

Zhiguo Gao, Ping Xue, Linmeng Ma, Keren Shi, Haibo Wang

Microbial fuel cells (MFCs) have been widely used in the treatment of organic wastewater because of its advantages of high efficiency, environmental friendliness, and no secondary pollution. In this study, we developed a composite anode, CeO₂@Co₃O₄-PEDOT/CF (PEDOT: poly(3,4-ethylenedioxythiophene), CF: carbon felt), by incorporating bimesoporous CeO₂@Co₃O₄ nanoparticles and PEDOT layer. The MFCs equipped with this novel anode had demonstrated exceptional catalytic performance in the treatment of Polyoxymethylene (POM) wastewater. Specifically, it achieved a 74.9 % increase in output voltage (586 ± 23 mV), a 2.5-fold increase in power density (3564 ± 27 mW/m²), and enhancements of 41.8 %, 40.1 %, and 47.0 % in the degradation rates of formaldehyde (HCHO, 97.2 %), 1,3,5-trioxane (TOX, 93.6 %), and chemical oxygen demand (COD) removal efficiency (84.4 %), respectively, when compared to a bare CF anode. Furthermore, the MFC with the composite anode demonstrated high degradation efficiency and COD removal efficiency across various industrial water environments. Additionally, the wheat seed germination experiments indicated a significant reduction in the toxicity of the degraded solution. This enhanced performance could be attributed to the synergistic effects of the bimesoporous CeO₂@Co₃O₄ nanoparticles and the PEDOT layer. This study presents a promising strategy for the degradation of organic pollutants and the generation of bioelectricity in practical applications.

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引用次数: 0

Promotion of quiescence and maintenance of function of mesenchymal stem cells on substrates with surface potential

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-01-31 DOI: 10.1016/j.bioelechem.2025.108920

Xiaoshuai Peng , Guojian Li , Jiu Zhao , Huatao Liu , Changhua Wu , Zepeng Su , Zhidong Liu , Shuai Fan , Yuanquan Chen , Yanfeng Wu , Wenjie Liu , Huiyong Shen , Guan Zheng

The widespread use of human mesenchymal stem cells(hMSCs) is impeded by functional loss during prolonged expansion. Although multiple approaches have been attempted to preserve hMSCs stemness, a suitable culture system remains to be modified. The interaction between electrical signals and stem cells is expected to better maintain the function of stem cells. However, it remains unclear whether the surface potential of substrates has the potential to preserve stem cell function during in vitro expansion. In our study, hMSCs cultured on materials with different surface potentials could be induced into a reversible quiescent state, and we demonstrated that quiescent hMSCs could be reactivated and transitioned back into the proliferation cell cycle. hMSCs cultured under appropriate potential displayed superior differentiation and proliferation abilities within the same generation compared to conventional conditions. These findings underscore the importance of surface potential as a critical physical factor regulating hMSCs stemness. Manipulating the surface potential of hMSCs culture substrates holds promise for optimising preservation and culture conditions, thereby enhancing their application in tissue repair and regeneration engineering.

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引用次数: 0

Electrochemical and computational studies on the interaction between calf-thymus DNA and skin whitening agent arbutin

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-01-30 DOI: 10.1016/j.bioelechem.2025.108923

Maryam M.M. Mahdi, Alper Fitoz, Ceren Yıldız, Dilek Eskiköy Bayraktepe, Zehra Yazan

The interaction between double-stranded calf thymus DNA (ctDNA) and the skin whitening agent arbutin (AR) examined by applying electrochemical and computational methods for the first time in literature. A single-use pencil graphite electrode via cyclic (CV) and differential pulse voltammetry (DPV) techniques were applied to determine the kinetic and thermodynamic parameters in the absence and presence of ctDNA. To examine the interaction process, oxidation peak currents and potentials of AR were observed prior to the addition of various ctDNA concentrations. The binding constants (K_AR-DNA) and Gibbs free energy (ΔG°) values for the AR-DNA complex were determined as 1.82 × 10⁴ L/mol and −24.30 kJ/mol at 298 K, respectively. Temperature evaluation of the interaction was examined using thermodynamic parameters (ΔH°: −30.30 kJ/mol and ΔS°: −0.00197 kJ/mol) applying the Van’t Hoff equation. The local interaction sites in the molecule structure were determined by applying Fukui functions and second-order perturbation theory in view of potential hydrogen binding centers. The optimized structure of AR was applied with a DNA structure revealing the binding position for AR-DNA complex. Experimental and computational examinations suggested that AR-DNA binds to ctDNA through a minor groove mode via conventional hydrogen bonds, hydrophobic interactions and van der Waals forces.

{"title":"Electrochemical and computational studies on the interaction between calf-thymus DNA and skin whitening agent arbutin","authors":"Maryam M.M. Mahdi, Alper Fitoz, Ceren Yıldız, Dilek Eskiköy Bayraktepe, Zehra Yazan","doi":"10.1016/j.bioelechem.2025.108923","DOIUrl":"10.1016/j.bioelechem.2025.108923","url":null,"abstract":"<div><div>The interaction between double-stranded calf thymus DNA (<em>ctDNA</em>) and the skin whitening agent arbutin (<em>AR</em>) examined by applying electrochemical and computational methods for the first time in literature. A single-use pencil graphite electrode via cyclic (<em>CV</em>) and differential pulse voltammetry (<em>DPV</em>) techniques were applied to determine the kinetic and thermodynamic parameters in the absence and presence of <em>ctDNA</em>. To examine the interaction process, oxidation peak currents and potentials of <em>AR</em> were observed prior to the addition of various <em>ctDNA</em> concentrations. The binding constants (<em>K<sub>AR-DNA</sub></em>) and Gibbs free energy (Δ<em>G°</em>) values for the <em>AR-DNA</em> complex were determined as <em>1.82 × 10</em><sup>4</sup> <em>L/mol</em> and <em>−24.30 kJ/mol</em> at 298 K, respectively. Temperature evaluation of the interaction was examined using thermodynamic parameters (Δ<em>H°: −30.30 kJ/mol</em> and Δ<em>S°: −0.00197 kJ/mol</em>) applying the Van’t Hoff equation. The local interaction sites in the molecule structure were determined by applying Fukui functions and second-order perturbation theory in view of potential hydrogen binding centers. The optimized structure of <em>AR</em> was applied with a <em>DNA</em> structure revealing the binding position for <em>AR-DNA</em> complex. Experimental and computational examinations suggested that <em>AR-DNA</em> binds to <em>ctDNA</em> through a minor groove mode via conventional hydrogen bonds, hydrophobic interactions and van der Waals forces.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108923"},"PeriodicalIF":4.8,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing water toxicity determination sensitivity by using TMAO as electron acceptor of inward extracellular electron transfer in electrochemically active bacteria

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-01-30 DOI: 10.1016/j.bioelechem.2025.108925

Yuxuan Zang , Bo Cao , Xuemei Yi , Fan Zha , Yanhong Ge , Hong Liu , Yue Yi

Toxicity determination based on electrochemically active bacteria (EAB) shows great prospects for early warning of sudden water pollution. However, the main bottleneck for practical application is the low sensitivity. Extracellular electron transfer (EET) is a key parameter influencing sensitivity. Our previous research has demonstrated that EAB exhibit higher sensitivity when performing inward EET compared with outward EET. Inward EET relies on electron acceptors, but the effects of electron acceptors on sensitivity remain unclear. In this study, the sensitivity of toxicity determination with different electron acceptors was compared. Results indicated that the choice of electron acceptors significantly changed the sensitivity. When Trimethylamine N-oxide (TMAO) was chosen as the electron acceptor, EAB exhibited the highest sensitivity, with a lower response limit of 0.05 mg/L Cd²⁺. The main reason was that the utilization of TMAO for inward EET increases the membrane permeability of EAB cells, facilitates toxic pollutant penetration, and results in high mortality after toxicity exposure.

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引用次数: 0

Whole-cell redox biosensor for triclosan detection: Integrating spectrophotometric and electrochemical detection

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-01-28 DOI: 10.1016/j.bioelechem.2025.108921

Himanshu Khandelwal , Sakuntala Mutyala , Minsoo Kim , Da Seul Kong , Jung Rae Kim

Organic pollutants like bisphenol, acetaminophen, and triclosan, widely used in healthcare products, pose environmental risks and act as endocrine disruptors. These pollutants can alter the intracellular redox balance, making engineered whole-cell redox biosensors valuable for their detection. This study utilized the SoxRS regulatory system in bacteria, which responds to oxidative stress through NADP⁺/NADPH levels by modulating gene expression of SoxS through the SoxS promoter (pSoxS). A plasmid containing SoxR-pSoxS and the LacZ reporter gene was constructed and introduced into E. coli BL21 (ΔLacZ SoxRS+). The LacZ gene enabled dual detection using O-nitrophenyl-β-galactopyranoside (ONPG) for spectrophotometric detection or p-aminophenyl β-D-galactopyranoside (PAPG) for electrochemical detection. The whole-cell pRUSL12 redox biosensor was activated by redox inducers such as pyocyanin and methyl viologen, measurable via β-galactosidase assays. Among pollutants tested, triclosan specifically repressed SoxR:pSoxS::lacZ activity in the presence of pyocyanin or methyl viologen. Optimization identified pyocyanin as the more effective inducer for triclosan detection, with the biosensor capable of detecting triclosan in the 100–400 µg/L range. These redox-based biosensors offer a powerful tool for monitoring metabolic redox changes and identifying specific organic pollutants in the environment.

{"title":"Whole-cell redox biosensor for triclosan detection: Integrating spectrophotometric and electrochemical detection","authors":"Himanshu Khandelwal , Sakuntala Mutyala , Minsoo Kim , Da Seul Kong , Jung Rae Kim","doi":"10.1016/j.bioelechem.2025.108921","DOIUrl":"10.1016/j.bioelechem.2025.108921","url":null,"abstract":"<div><div>Organic pollutants like bisphenol, acetaminophen, and triclosan, widely used in healthcare products, pose environmental risks and act as endocrine disruptors. These pollutants can alter the intracellular redox balance, making engineered whole-cell redox biosensors valuable for their detection. This study utilized the SoxRS regulatory system in bacteria, which responds to oxidative stress through NADP<sup>+</sup>/NADPH levels by modulating gene expression of SoxS through the SoxS promoter (pSoxS). A plasmid containing SoxR-pSoxS and the LacZ reporter gene was constructed and introduced into <em>E. coli</em> BL21 (ΔLacZ SoxRS+). The LacZ gene enabled dual detection using O-nitrophenyl-β-galactopyranoside (ONPG) for spectrophotometric detection or p-aminophenyl β-D-galactopyranoside (PAPG) for electrochemical detection. The whole-cell pRUSL12 redox biosensor was activated by redox inducers such as pyocyanin and methyl viologen, measurable via β-galactosidase assays. Among pollutants tested, triclosan specifically repressed SoxR:pSoxS::lacZ activity in the presence of pyocyanin or methyl viologen. Optimization identified pyocyanin as the more effective inducer for triclosan detection, with the biosensor capable of detecting triclosan in the 100–400 µg/L range. These redox-based biosensors offer a powerful tool for monitoring metabolic redox changes and identifying specific organic pollutants in the environment.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108921"},"PeriodicalIF":4.8,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143155966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bidirectional electro-enzymatic reaction of coenzyme F420 using benzyl viologen and F420-dependent sulfite reductase

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-01-28 DOI: 10.1016/j.bioelechem.2025.108922

Satoshi Furota , Masanori Kaneko , Seiya Tsujimura , Daijiro Takeshita , Yusuke Nakamichi , Kensuke Igarashi , Masaru K. Nobu , Miho Yoshikawa , Kenta Asahina , Chie Fukaya , Toshie Ishitsuka , Kazuma Shimada

Coenzyme F₄₂₀ is recognized as a crucial electron carrier in methane-generating metabolism but, beyond this, has garnered significant attention for its role in diverse microbial physiologies and relevance in industrial, medical, and environmental applications. However, one limitation of current application of F₄₂₀ is the necessity of chemical electron donors for its reduction. In this study, an electrochemical reaction system was designed to facilitate electron transfer between the electrode and F₄₂₀ using F₄₂₀-dependent sulfite reductase (Fsr) as the catalyst and benzyl viologen (BV) as the redox mediator. Photometric analysis and cyclic potential scanning demonstrated that the occurrence of bidirectional (reversible) electrochemical oxidation and reduction of F₄₂₀ in this system depended on the electrode potential. The formal redox potential of F₄₂₀ in this system was −540 mV vs. Ag|AgCl|sat. KCl, which aligned with values previously determined using biochemical assays.

{"title":"Bidirectional electro-enzymatic reaction of coenzyme F420 using benzyl viologen and F420-dependent sulfite reductase","authors":"Satoshi Furota , Masanori Kaneko , Seiya Tsujimura , Daijiro Takeshita , Yusuke Nakamichi , Kensuke Igarashi , Masaru K. Nobu , Miho Yoshikawa , Kenta Asahina , Chie Fukaya , Toshie Ishitsuka , Kazuma Shimada","doi":"10.1016/j.bioelechem.2025.108922","DOIUrl":"10.1016/j.bioelechem.2025.108922","url":null,"abstract":"<div><div>Coenzyme F<sub>420</sub> is recognized as a crucial electron carrier in methane-generating metabolism but, beyond this, has garnered significant attention for its role in diverse microbial physiologies and relevance in industrial, medical, and environmental applications. However, one limitation of current application of F<sub>420</sub> is the necessity of chemical electron donors for its reduction. In this study, an electrochemical reaction system was designed to facilitate electron transfer between the electrode and F<sub>420</sub> using F<sub>420</sub>-dependent sulfite reductase (Fsr) as the catalyst and benzyl viologen (BV) as the redox mediator. Photometric analysis and cyclic potential scanning demonstrated that the occurrence of bidirectional (reversible) electrochemical oxidation and reduction of F<sub>420</sub> in this system depended on the electrode potential. The formal redox potential of F<sub>420</sub> in this system was −540 mV vs. Ag|AgCl|sat. KCl, which aligned with values previously determined using biochemical assays.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108922"},"PeriodicalIF":4.8,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of boron doped diamond electrodes with engineered sp2 carbon content and their application to structure-dependent DNA hybridization

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-01-27 DOI: 10.1016/j.bioelechem.2025.108910

Ondrej Hesko , Hana Pivoňková , Lukáš Fojt , Andrew Taylor , Jaromír Kopeček , Karolina Schwarzová-Pecková , Miroslav Fojta

Boron doped diamond electrodes brought a new potential in bioanalytical chemistry including studies of structure and interactions of nucleic acids. Herein, deposition conditions were optimized to produce a set of polycrystalline BDD electrodes with comparable boron concentration in solid phase of (1.8 − 2.1) · 10²¹ cm⁻³ akin to metallic-type conductivity but with increasing sp² carbon content. Increase of [CH₄]/[H₂] from 0.25 % to 2.0 % during deposition led to an obvious decrease in grain size from ca. 300 nm (BDD_0.25) to < 100 nm (BDD_2.0). Adsorption of oligodeoxynucleotides and their structural changes in the presence of K⁺ and Li⁺ ions were evaluated through enzyme-linked DNA hybridization assay in which oxidizable 1-naphthol was released from its phosphoester by streptavidin–alkaline phosphatase conjugate upon successful hybridization of the target oligodeoxynucleotide with a biotinylated complementary probe. With increasing sp² carbon content, the hybridization assay showed improved discrimination between a target forming guanine quadruplex (stabilized by K⁺ ions), yielding by 40 % – 60 % lower hybridization signal with the complementary probe, compared to the same but unstructured target oligodeoxynucleotide in the presence of Li⁺ ions that don’t stabilize the quadruplex structure. Such behaviour was observed also for commercial BDD electrode with surface roughness < 10 nm.

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引用次数: 0

Label-free electrochemical immunosensors based on Cu-Ni metal–organic framework and carbon nanotube composite for carcinoembryonic antigen detection

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-01-27 DOI: 10.1016/j.bioelechem.2025.108918

Shi Wang , Jialin Ao , Saiwen Ding , Ting Shu

Monitoring cancer biomarkers is of great significance in clinical diagnosis. In this work, a label-free MWCNTs-COOH/CuNi-BTC/FTO electrochemical immunosensor was developed to quantitatively detect carcinoembryonic antigen (CEA). The bimetallic CuNi-BTC showed enhanced current than singe Ni-BTC, and the addition of the MWCNTs-COOH increased the conductivity and further amplified the current signal. The electrode was further modified with CEA antigen (Ag) and bovine serum albumin (BSA) was used to block the non-specific binding sites. Using the emplified current signal of CuNi-BTC, CEA was detected by a DPV method through the current change caused by the specific recognition reaction of Ag and Ab. Under optimal conditions, a range of 0.80–140 ng/mL and a detection limit of 0.046 ng/mL for CEA was obtained. This electrochemical immunosensor possessed good selectivity, reproducibility and long-term stability.

引用次数: 0

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