Pub Date : 2025-02-25DOI: 10.1016/j.bioelechem.2025.108952
Fuyan Kang , Guangyu Zhu , Xiaoya Ding , Yabei Zhang , Zilong Zhao , Tao Zhang , Zhongyi He , Fa-Qian Liu
Marine biofouling and corrosion are serious impediments to the promotion and development of the marine industry. The short service life and limited application of single coatings have greatly increased the economic burden on the industry. The development of multi-functional composite coatings has become a particularly pressing issue. The combination of Cu-BTC and Ti3C2Tx as a specific resin filler represents a new strategy (Ti3C2Tx@Cu-BTC@EP). HAADF-STEM, PXRD and XPS were used to verify the successful synthesis of the materials. Ti3C2Tx@Cu-BTC@EP was able to achieve 100 % lethality of E. coli under the condition of light exposure within 24 h. In addition, the impedance modulus of the coating in the low-frequency range was increased by about 3.15 times compared to the blank group with the addition of 1 wt% filler, reaching as high as 7.06 × 108 Ω. Overall, the novel Ti3C2Tx@Cu-BTC@EP composite coating is expected to promote new advances in epoxy resin research.
{"title":"In situ growth of two-dimensional MXene/Nano-copper metal-organic framework composites for antimicrobial applications in epoxy coatings","authors":"Fuyan Kang , Guangyu Zhu , Xiaoya Ding , Yabei Zhang , Zilong Zhao , Tao Zhang , Zhongyi He , Fa-Qian Liu","doi":"10.1016/j.bioelechem.2025.108952","DOIUrl":"10.1016/j.bioelechem.2025.108952","url":null,"abstract":"<div><div>Marine biofouling and corrosion are serious impediments to the promotion and development of the marine industry. The short service life and limited application of single coatings have greatly increased the economic burden on the industry. The development of multi-functional composite coatings has become a particularly pressing issue. The combination of Cu-BTC and Ti<sub>3</sub>C<sub>2</sub>Tx as a specific resin filler represents a new strategy (Ti<sub>3</sub>C<sub>2</sub>Tx@Cu-BTC@EP). HAADF-STEM, PXRD and XPS were used to verify the successful synthesis of the materials. Ti<sub>3</sub>C<sub>2</sub>Tx@Cu-BTC@EP was able to achieve 100 % lethality of <em>E. coli</em> under the condition of light exposure within 24 h. In addition, the impedance modulus of the coating in the low-frequency range was increased by about 3.15 times compared to the blank group with the addition of 1 wt% filler, reaching as high as 7.06 × 10<sup>8</sup> Ω. Overall, the novel Ti<sub>3</sub>C<sub>2</sub>Tx@Cu-BTC@EP composite coating is expected to promote new advances in epoxy resin research.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108952"},"PeriodicalIF":4.8,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-24DOI: 10.1016/j.bioelechem.2025.108949
Denise Demurtas , Julia Alvarez-Malmagro , Arvind Rathore , Tanushree Mandal , Andrés Felipe Quintero-Jaime , Serguei Belochapkine , Anna Lielpetere , Kavita Jayakumar , Dónal Leech , Wolfgang Schuhmann , Nicolas Mano , Claudine Boiziau , Edmond Magner
The development of implantable glucose sensors is of significant interest in the management of diabetes. This work focuses on developing an implantable, biocompatible nanoporous gold electrode prototype based on Kapton® for the subcutaneous detection of glucose. The electrodes were first modified with a layer containing glucose oxidase and Os(2,2′-bipyridine)2Cl·PVI (Os(bpy)2Cl PVI). An additional polymeric layer containing poly(2-methacryloyloxyethyl phosphorylcholine-co-glycidyl methacrylate) was then added to reduce biofouling and foreign body reaction effects. The modified electrode had a VMAX of 211 ± 13 μA cm−2 and a KMapp of 6.1 ± 0.8 mM in pseudo physiological conditions, with a linear detection range from 1 to 4 mM and a sensitivity of 28.6 ± 2.1 μA cm−2 mM−1. In artificial plasma, the response of the sensor was saturated at 3 mM, with a VMAX of 113 ± 10 μA cm−2 and a KMapp of 2.1 ± 0.4 mM with a linear detection range from 1 to 2.5 mM and a sensitivity of 14.6 ± 3.3 μA cm−2 mM−1. Mechanical stress testing demonstrated that there was a 40 % reduction of the redox polymer coverage after 320 deformation events, however the catalytic activity was still detectable after 160 events. Minimal cytotoxicity effects of the electrodes were observed. When subcutaneously implanted the electrodes showed fairly good mechanical stability after one week and detachment of the metallic layer on some electrodes after 21 days, probably due to electrode bending. A limited foreign body reaction was observed. These results indicated that the electrodes could be implanted for a period of up to 1 week.
{"title":"Development of flexible nanoporous gold electrodes for the detection of glucose","authors":"Denise Demurtas , Julia Alvarez-Malmagro , Arvind Rathore , Tanushree Mandal , Andrés Felipe Quintero-Jaime , Serguei Belochapkine , Anna Lielpetere , Kavita Jayakumar , Dónal Leech , Wolfgang Schuhmann , Nicolas Mano , Claudine Boiziau , Edmond Magner","doi":"10.1016/j.bioelechem.2025.108949","DOIUrl":"10.1016/j.bioelechem.2025.108949","url":null,"abstract":"<div><div>The development of implantable glucose sensors is of significant interest in the management of diabetes. This work focuses on developing an implantable, biocompatible nanoporous gold electrode prototype based on Kapton® for the subcutaneous detection of glucose. The electrodes were first modified with a layer containing glucose oxidase and Os(2,2′-bipyridine)<sub>2</sub>Cl·PVI (Os(bpy)<sub>2</sub>Cl PVI). An additional polymeric layer <em>co</em>ntaining poly(2-methacryloyloxyethyl phosphorylcholine-co-glycidyl methacrylate) was then added to reduce biofouling and foreign body reaction effects. The modified electrode had a V<sub>MAX</sub> of 211 ± 13 μA cm<sup>−2</sup> and a K<sub>Mapp</sub> of 6.1 ± 0.8 mM in pseudo physiological conditions, with a linear detection range from 1 to 4 mM and a sensitivity of 28.6 ± 2.1 μA cm<sup>−2</sup> mM<sup>−1</sup>. In artificial plasma, the response of the sensor was saturated at 3 mM, with a V<sub>MAX</sub> of 113 ± 10 μA cm<sup>−2</sup> and a K<sub>Mapp</sub> of 2.1 ± 0.4 mM with a linear detection range from 1 to 2.5 mM and a sensitivity of 14.6 ± 3.3 μA cm<sup>−2</sup> mM<sup>−1</sup>. Mechanical stress testing demonstrated that there was a 40 % reduction of the redox polymer coverage after 320 deformation events, however the catalytic activity was still detectable after 160 events. Minimal cytotoxicity effects of the electrodes were observed. When subcutaneously implanted the electrodes showed fairly good mechanical stability after one week and detachment of the metallic layer on some electrodes after 21 days, probably due to electrode bending. A limited foreign body reaction was observed. These results indicated that the electrodes could be implanted for a period of up to 1 week.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108949"},"PeriodicalIF":4.8,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-22DOI: 10.1016/j.bioelechem.2025.108941
Philip N. Bartlett, M. Hashim Khan
There has been an enormous increase in the computational power readily available since the first numerical treatments of electrochemical problems in the early 1960s. This development has been accompanied by the development of powerful, widely available, commercial software modelling tools. Despite this, approximate analytical treatments remain extremely useful in the modelling of coupled diffusion/reaction problems in electrochemistry because of the insights they provide into the different possible behaviours of the system. In this paper we discuss the modelling of amperometric enzyme electrodes, taking as our exemplar redox hydrogel-based enzyme electrodes in which the enzyme is immobilized in a redox active polymer which wires the enzyme to the electrode. In this system the measured current is related to many different experimental variables including substrate concentration and diffusion coefficient, reaction rate constants, and film properties and thickness. The interplay of these factors is described and the role of Case diagrams in understanding coupled diffusion/reaction problems of this type is discussed.
{"title":"Modelling enzyme electrodes – What do we learn and how is it useful?","authors":"Philip N. Bartlett, M. Hashim Khan","doi":"10.1016/j.bioelechem.2025.108941","DOIUrl":"10.1016/j.bioelechem.2025.108941","url":null,"abstract":"<div><div>There has been an enormous increase in the computational power readily available since the first numerical treatments of electrochemical problems in the early 1960s. This development has been accompanied by the development of powerful, widely available, commercial software modelling tools. Despite this, approximate analytical treatments remain extremely useful in the modelling of coupled diffusion/reaction problems in electrochemistry because of the insights they provide into the different possible behaviours of the system. In this paper we discuss the modelling of amperometric enzyme electrodes, taking as our exemplar redox hydrogel-based enzyme electrodes in which the enzyme is immobilized in a redox active polymer which wires the enzyme to the electrode. In this system the measured current is related to many different experimental variables including substrate concentration and diffusion coefficient, reaction rate constants, and film properties and thickness. The interplay of these factors is described and the role of Case diagrams in understanding coupled diffusion/reaction problems of this type is discussed.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108941"},"PeriodicalIF":4.8,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-21DOI: 10.1016/j.bioelechem.2025.108944
Parastoo Vahdatiyekta , Ville Yrjänä , Emil Rosqvist , Xavier Cetó , Manel del Valle , Tan-Phat Huynh
This study evaluates different electrochemical behaviors of modified glassy carbon electrodes (GCEs) for detecting urinary biomarkers related to breast cancer, namely homovanillic acid (HVA) and 4-hydroxyphenylacetic acid (4HPA). The analysis was performed in the presence of common urinary interferents, creatinine and urea. Modification of bare GCEs was done through the electropolymerization of N,N′-bis (2-thienylmethylene)-1,X-diaminobenzene (X = 2, 3, 4) isomers, so-called BTMD. The formation and characteristics of these polymeric layers were investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), atomic force microscopy (AFM), and scanning electron microscopy (SEM). Differential pulse voltammetry (DPV) was used to measure responses of the electrodes to HVA and 4HPA, assessing their sensitivity and selectivity. Results showed that the developed electrodes effectively detected both biomarkers, with peak currents increasing proportionally to biomarker concentrations and minimal interference from creatinine and urea. The modified electrodes exhibited better linearity at higher concentrations; however, saturation was observed for 4HPA at high concentrations with the p-BTMD/GCE. Each electrode displayed unique peak current, potential, and response profiles, highlighting their promise for cross-reactive sensing systems, such as electronic tongues, to analyze complex matrices such as urine.
{"title":"Electrochemical Behavior of Glassy Carbon Electrodes Modified with Electropolymerized Film of N,N′-bis (2-thienylmethylene)-1,X-diaminobenzene toward Homovanillic Acid and 4-Hydroxyphenylacetic Acid","authors":"Parastoo Vahdatiyekta , Ville Yrjänä , Emil Rosqvist , Xavier Cetó , Manel del Valle , Tan-Phat Huynh","doi":"10.1016/j.bioelechem.2025.108944","DOIUrl":"10.1016/j.bioelechem.2025.108944","url":null,"abstract":"<div><div>This study evaluates different electrochemical behaviors of modified glassy carbon electrodes (GCEs) for detecting urinary biomarkers related to breast cancer, namely homovanillic acid (HVA) and 4-hydroxyphenylacetic acid (4HPA). The analysis was performed in the presence of common urinary interferents, creatinine and urea. Modification of bare GCEs was done through the electropolymerization of <em>N,N′-</em>bis (2-thienylmethylene)-1<em>,X-</em>diaminobenzene (<em>X =</em> 2, 3, 4) isomers, so-called BTMD. The formation and characteristics of these polymeric layers were investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), atomic force microscopy (AFM), and scanning electron microscopy (SEM). Differential pulse voltammetry (DPV) was used to measure responses of the electrodes to HVA and 4HPA, assessing their sensitivity and selectivity. Results showed that the developed electrodes effectively detected both biomarkers, with peak currents increasing proportionally to biomarker concentrations and minimal interference from creatinine and urea. The modified electrodes exhibited better linearity at higher concentrations; however, saturation was observed for 4HPA at high concentrations with the <em>p</em>-BTMD/GCE. Each electrode displayed unique peak current, potential, and response profiles, highlighting their promise for cross-reactive sensing systems, such as electronic tongues, to analyze complex matrices such as urine.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108944"},"PeriodicalIF":4.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-21DOI: 10.1016/j.bioelechem.2025.108946
Petra Hellwig
Terminal oxidases are critical for aerobic respiratory chains of prokaryotes and eukaryotes, responsible for the final step in the electron transport chain. These enzymes catalyze the transfer of electrons from reduced electron carriers (such as cytochrome c or quinols) to the terminal electron acceptor, molecular oxygen (O₂), thereby reducing it to water. They play a pivotal role in aerobic respiration and energy metabolism, adapting to diverse environmental and physiological needs across different organisms. This review summarizes the electrochemical properties of terminal oxidases from different organisms and reveals their high degree of adaptivity with redox potentials spanning more than 500 mV. The electrocatalytic response in direct electrochemical approaches is described giving insight into the rich and complex electron and proton transfer catalysed by these essential enzymes.
{"title":"The electrochemical properties of the highly diverse terminal oxidases from different organisms","authors":"Petra Hellwig","doi":"10.1016/j.bioelechem.2025.108946","DOIUrl":"10.1016/j.bioelechem.2025.108946","url":null,"abstract":"<div><div>Terminal oxidases are critical for aerobic respiratory chains of prokaryotes and eukaryotes, responsible for the final step in the electron transport chain. These enzymes catalyze the transfer of electrons from reduced electron carriers (such as cytochrome <em>c</em> or quinols) to the terminal electron acceptor, molecular oxygen (O₂), thereby reducing it to water. They play a pivotal role in aerobic respiration and energy metabolism, adapting to diverse environmental and physiological needs across different organisms. This review summarizes the electrochemical properties of terminal oxidases from different organisms and reveals their high degree of adaptivity with redox potentials spanning more than 500 mV. The electrocatalytic response in direct electrochemical approaches is described giving insight into the rich and complex electron and proton transfer catalysed by these essential enzymes.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108946"},"PeriodicalIF":4.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study presents a novel, label-free electrochemical immunosensor for the detection of vascular endothelial growth factor (VEGF), a crucial tumor biomarker. The immunosensor was developed by electrochemical deposition of gold nanoparticles-reduced graphene oxide (AuNPs-rGO) nanocomposite on glassy carbon (GC) and screen-printed carbon (SPC) electrodes. A specific monoclonal antibody against VEGF was immobilized on the electrode surface through a carbodiimide coupling reaction. Field Emission Scanning Electron Microscopy (FE-SEM), X-ray Diffraction (XRD), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) have been used to characterize the developed immunosensor. For quantitative measurement of VEGF, fast Fourier transformation admittance voltammetry was employed by applying a special potential waveform on the immunosensor and sampling the currents. The response was determined by measuring changes in the electrode admittance caused by the adsorption of VEGF molecules, without the use of a redox probe. Under optimal conditions, the immunosensor responses were within a linear detection range for VEGF from 0.1 to 10,000 pg/ml and from 10 to 10,000 pg/ml, with notably low detection limits of 29.1 fg/ml and 352 fg/ml for the modified GC and SPC electrodes, respectively. The sensor exhibits minimal interference from common serum proteins, making it a promising candidate for sensitive, low-cost commercialization.
{"title":"A novel label-free immunosensor for detection of VEGF using FFT admittance voltammetry","authors":"Negar Heidari , Reza Hassan Sajedi , Ali Nemati Kharat , Alireza Bonakdar , Jalil Mirzazadeh , Kianoush Kazemi Qaraei , Parviz Norouzi","doi":"10.1016/j.bioelechem.2025.108948","DOIUrl":"10.1016/j.bioelechem.2025.108948","url":null,"abstract":"<div><div>This study presents a novel, label-free electrochemical immunosensor for the detection of vascular endothelial growth factor (VEGF), a crucial tumor biomarker. The immunosensor was developed by electrochemical deposition of gold nanoparticles-reduced graphene oxide (AuNPs-rGO) nanocomposite on glassy carbon (GC) and screen-printed carbon (SPC) electrodes. A specific monoclonal antibody against VEGF was immobilized on the electrode surface through a carbodiimide coupling reaction. Field Emission Scanning Electron Microscopy (FE-SEM), X-ray Diffraction (XRD), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) have been used to characterize the developed immunosensor. For quantitative measurement of VEGF, fast Fourier transformation admittance voltammetry was employed by applying a special potential waveform on the immunosensor and sampling the currents. The response was determined by measuring changes in the electrode admittance caused by the adsorption of VEGF molecules, without the use of a redox probe. Under optimal conditions, the immunosensor responses were within a linear detection range for VEGF from 0.1 to 10,000 pg/ml and from 10 to 10,000 pg/ml, with notably low detection limits of 29.1 fg/ml and 352 fg/ml for the modified GC and SPC electrodes, respectively. The sensor exhibits minimal interference from common serum proteins, making it a promising candidate for sensitive, low-cost commercialization.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108948"},"PeriodicalIF":4.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-19DOI: 10.1016/j.bioelechem.2025.108945
Tingting Tian , Lixia Chen , Taiguang Li , Xiang Wang , Sen Yang , Huiqun Wang , Yujie Jiang , Xin Yao , Hong Zhao , Dengchao Wang , Xiangjun Li
Electrochemiluminescence (ECL) is highly recommended in chiral recognition. ECL-based chiral sensors highly desire a sensitive sensing interface for signal conversion and absolute chiral discrimination. The ECL emission based on a luminol-dissolved O2 system received much attention due to its nontoxicity and stability. However, the drawback of weak ECL emission hinders the fast signal conversion from chiral discrimination to ECL response. Herein, the amplification strategy of ECL emission is proposed based on the electrochemical reduction enhanced O2 reduction reaction (ORR). Cadmium sulfide decorated on carbon-nanotubes (CdS/CNTs) with easy synthesis, wide-pH operation, and suitable valence-conduction band position is employed. Upon cathodic scan, the electrons transfer from electrochemically reduced-CdS/CNTs to O2 and H2O2, thus accelerating the generation of reactive oxide species (ROS) and furthering ECL emission. Furthermore, the chiral ECL sensing interface is well-designed by combining the chiral recognition of D-amino acid oxidase (DAAO) with the signal transduction and amplification of CdS/CNTs-enhanced ECL emission. During DAAO-catalyzed enantioselective-oxidations of alanine, the O2 is converted to H2O2, which tunes the ROS generation. With synergetic regulations of ROS generation by nano-derived CdS/CNTs and bio-derived DAAO, alanine enantiomers are highly discriminated and the L-alanine is quantitatively detected with the most competitive detection limit so far (0.014 fM).
{"title":"Electrochemical reduction boosted Luminol cathodic electrochemiluminescence for trace chiral recognition of alanine enantiomers","authors":"Tingting Tian , Lixia Chen , Taiguang Li , Xiang Wang , Sen Yang , Huiqun Wang , Yujie Jiang , Xin Yao , Hong Zhao , Dengchao Wang , Xiangjun Li","doi":"10.1016/j.bioelechem.2025.108945","DOIUrl":"10.1016/j.bioelechem.2025.108945","url":null,"abstract":"<div><div>Electrochemiluminescence (ECL) is highly recommended in chiral recognition. ECL-based chiral sensors highly desire a sensitive sensing interface for signal conversion and absolute chiral discrimination. The ECL emission based on a luminol-dissolved O<sub>2</sub> system received much attention due to its nontoxicity and stability. However, the drawback of weak ECL emission hinders the fast signal conversion from chiral discrimination to ECL response. Herein, the amplification strategy of ECL emission is proposed based on the electrochemical reduction enhanced O<sub>2</sub> reduction reaction (ORR). Cadmium sulfide decorated on carbon-nanotubes (CdS/CNTs) with easy synthesis, wide-pH operation, and suitable valence-conduction band position is employed. Upon cathodic scan, the electrons transfer from electrochemically reduced-CdS/CNTs to O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub>, thus accelerating the generation of reactive oxide species (ROS) and furthering ECL emission. Furthermore, the chiral ECL sensing interface is well-designed by combining the chiral recognition of D-amino acid oxidase (DAAO) with the signal transduction and amplification of CdS/CNTs-enhanced ECL emission. During DAAO-catalyzed enantioselective-oxidations of alanine, the O<sub>2</sub> is converted to H<sub>2</sub>O<sub>2</sub>, which tunes the ROS generation. With synergetic regulations of ROS generation by nano-derived CdS/CNTs and bio-derived DAAO, alanine enantiomers are highly discriminated and the L-alanine is quantitatively detected with the most competitive detection limit so far (0.014 fM).</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108945"},"PeriodicalIF":4.8,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143480362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-14DOI: 10.1016/j.bioelechem.2025.108928
Tani Chen , Robert Langer , James C. Weaver
{"title":"Corrigendum to “Charged microbeads are not transported across the human stratum corneum in vitro by short high-voltage pulses” [Bioelectrochem. Bioenerget. 48(1) (1999) 181–192]","authors":"Tani Chen , Robert Langer , James C. Weaver","doi":"10.1016/j.bioelechem.2025.108928","DOIUrl":"10.1016/j.bioelechem.2025.108928","url":null,"abstract":"","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108928"},"PeriodicalIF":4.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-13DOI: 10.1016/j.bioelechem.2025.108942
Ding Guo , Jizhou Duan
In the seawater-sediment simulated immersion system, nitrate affected microbial corrosion of steel. The research studied the corrosion processes of Q235 steel influenced by nitrate exposure from aspects such as mineral evolution, environmental microbial cultivation, and interfacial electrochemistry. Nitrate pollution affected the corrosion acceleration (0.11 ± 0.01 mm*y−1, pitmax = 21.11 μm). Severe iron corrosion might not originate from the acidification of the interface microenvironment or the bioactivity of sulfate-reducing bacteria controlled by diffusion of inorganic nitrogen in the rust layer, but rather from the microbial metabolism of nitrate-reducing bacteria. The nitrate-addition had altered the composition of the microbial community attached to the steel surface, with a significant increase in the abundance of Achromobacter. The attached microorganisms regulated the Fe0 oxidation and the NO3− reduction on the Q235 steel surface to increase the pitting corrosion sensitivity and live bacteria number. The effect of nitrate on microbial corrosion of Fe0 in aerobic environment showed different understandings from the proposed corrosion phenomenological model.
{"title":"Nitrate pollution accelerated the microbial corrosion of Fe0: A simulated corrosion verification for understanding marine corrosion phenomenological model","authors":"Ding Guo , Jizhou Duan","doi":"10.1016/j.bioelechem.2025.108942","DOIUrl":"10.1016/j.bioelechem.2025.108942","url":null,"abstract":"<div><div>In the seawater-sediment simulated immersion system, nitrate affected microbial corrosion of steel. The research studied the corrosion processes of Q235 steel influenced by nitrate exposure from aspects such as mineral evolution, environmental microbial cultivation, and interfacial electrochemistry. Nitrate pollution affected the corrosion acceleration (0.11 ± 0.01 mm*y<sup>−1</sup>, pit<sub>max</sub> = 21.11 μm). Severe iron corrosion might not originate from the acidification of the interface microenvironment or the bioactivity of sulfate-reducing bacteria controlled by diffusion of inorganic nitrogen in the rust layer, but rather from the microbial metabolism of nitrate-reducing bacteria. The nitrate-addition had altered the composition of the microbial community attached to the steel surface, with a significant increase in the abundance of <em>Achromobacter</em>. The attached microorganisms regulated the Fe<sup>0</sup> oxidation and the NO<sub>3</sub><sup>−</sup> reduction on the Q235 steel surface to increase the pitting corrosion sensitivity and live bacteria number. The effect of nitrate on microbial corrosion of Fe<sup>0</sup> in aerobic environment showed different understandings from the proposed corrosion phenomenological model.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108942"},"PeriodicalIF":4.8,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号