Bioelectrochemistry最新文献_第2页

Laccase mimetics as sensing elements for amperometric assay of 5-hydroxyindoleacetic acid in urine 将漆酶模拟物作为安培法测定尿液中 5-羟基吲哚乙酸的传感元件。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-10-24 DOI: 10.1016/j.bioelechem.2024.108839

Olha Demkiv , Wojciech Nogala , Nataliya Stasyuk , Marcin Holdynski , Nina Dimcheva , Taras Danysh , Monika Asztemborska , Mykhailo Gonchar

Monitoring of the levels of 5-hydroxyindole-3-acetic acid (5-HIAA) is of significant importance for diagnostics of carcinoid tumors. We propose simple catalytic electrochemical sensors for the determination of 5-HIAA in urine using laccase and its mimetics. Laccase-like nanozymes (LacNZs) were synthesized via a chemical reduction, and resulting PtMn and MnO₂ nanoflowers (NFs) demonstrated laccase-like activity similar to the laccase from the Trametes zonata. In addition, these LacNZs showed enhanced stability under a wide range of pH (3.0–7.5), temperatures (4–70 °C), and ionic strengths (up to 500 mM NaCl). The developed PtMn NF/graphite electrode, similar to a laccase/graphite electrode, can detect 5-HIAA with a high sensitivity (25 000 ± 12 A·M⁻¹·m⁻² and 1900 ± 9 A·M⁻¹·m⁻², respectively) and have linear ranges of 0.3 – 15 μM and 2 – 50 μM. The sensors work at low working potentials with a detection limit of 0.16 and 1.4 μM, covering the normal and pathologic ranges of 5-HIAA (1 – 50 μM) content in urine. They have been successfully applied to 5-HIAA assay in urine samples of people with various diseases and revealed good recovery values and reproducibility. Additionally, the LacNZ-sensor has the best stability and can be used up to 20 days.

监测 5- 羟基吲哚-3-乙酸（5-HIAA）的水平对于类癌的诊断具有重要意义。我们提出了利用漆酶及其模拟物测定尿液中 5-HIAA 的简单催化电化学传感器。我们通过化学还原法合成了类似漆酶的纳米酶（LacNZs），所得到的铂锰和二氧化锰纳米花（NFs）表现出了类似漆酶的活性，其活性类似于来自瘤葡萄的漆酶。此外，这些 LacNZs 在广泛的 pH 值（3.0-7.5）、温度（4-70 °C）和离子强度（高达 500 mM NaCl）条件下显示出更强的稳定性。所开发的铂锰 NF/石墨电极类似于漆包线/石墨电极，可以高灵敏度（分别为 25 000 ± 12 A-M-1-m-2 和 1900 ± 9 A-M-1-m-2）检测 5-HIAA，线性范围分别为 0.3 - 15 μM 和 2 - 50 μM。传感器在低工作电位下工作，检测限分别为 0.16 和 1.4 μM，涵盖了尿液中 5-HIAA（1 - 50 μM）含量的正常和病理范围。它们已成功应用于各种疾病患者尿样中 5-HIAA 的检测，并显示出良好的回收率和重现性。此外，LacNZ 传感器的稳定性最好，可使用长达 20 天。

{"title":"Laccase mimetics as sensing elements for amperometric assay of 5-hydroxyindoleacetic acid in urine","authors":"Olha Demkiv , Wojciech Nogala , Nataliya Stasyuk , Marcin Holdynski , Nina Dimcheva , Taras Danysh , Monika Asztemborska , Mykhailo Gonchar","doi":"10.1016/j.bioelechem.2024.108839","DOIUrl":"10.1016/j.bioelechem.2024.108839","url":null,"abstract":"<div><div>Monitoring of the levels of 5-hydroxyindole-3-acetic acid (5-HIAA) is of significant importance for diagnostics of carcinoid tumors. We propose simple catalytic electrochemical sensors for the determination of 5-HIAA in urine using laccase and its mimetics. Laccase-like nanozymes (LacNZs) were synthesized <em>via</em> a chemical reduction, and resulting PtMn and MnO<sub>2</sub> nanoflowers (NFs) demonstrated laccase-like activity similar to the laccase from the <em>Trametes zonata</em>. In addition, these LacNZs showed enhanced stability under a wide range of pH (3.0–7.5), temperatures (4–70 °C), and ionic strengths (up to 500 mM NaCl). The developed PtMn NF/graphite electrode, similar to a laccase/graphite electrode, can detect 5-HIAA with a high sensitivity (25 000 ± 12 A·M<sup>−1</sup>·m<sup>−2</sup> and 1900 ± 9 A·M<sup>−1</sup>·m<sup>−2</sup>, respectively) and have linear ranges of 0.3 – 15 μM and 2 – 50 μM. The sensors work at low working potentials with a detection limit of 0.16 and 1.4 μM, covering the normal and pathologic ranges of 5-HIAA (1 – 50 μM) content in urine. They have been successfully applied to 5-HIAA assay in urine samples of people with various diseases and revealed good recovery values and reproducibility. Additionally, the LacNZ-sensor has the best stability and can be used up to 20 days.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108839"},"PeriodicalIF":4.8,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Demyelination detection in CSF based on electrochemical monitoring of myelin basic protein in comparison between Apta vs. Immuno sensing strategies 基于髓鞘碱性蛋白电化学监测的脑脊液脱髓鞘检测：Apta 与免疫传感策略的比较

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-10-18 DOI: 10.1016/j.bioelechem.2024.108834

Marina Serin , Pınar Kara

Multiple sclerosis (MS) is a recurrent inflammatory, demyelinating disease of the white matter in central nervous system (CNS). The number of MS patients is increasing, but the diagnostic process is still quite difficult, costly and requires combination of several methods. Myelin basic protein (MBP) makes up to 30 % of the myelin in CNS. It is known that MBP is released into the cerebrospinal fluid (CSF) as MS bioindicator. Herein, myelin specific DNA aptamer earlier developed for possible therapeutic purposes and anti-MBP antibody were applied as bioreceptors for MBP recognition on the same nanomodified sensor surfaces and their performances were compared. Biosensors were developed by using graphene oxide (GO) nanoparticles integrated onto pencil graphite electrodes (PGE) and bioreceptor molecules immobilized to create a bioactive layer for MBP binding. The measurements were run with electrochemical impedance spectroscopy (EIS). Selectivity of the biosensors was evaluated using human serum albumin (HSA). After optimization of binding parameters, biosensors were validated in artificial CSF. It was shown that LJM-5708 based aptasensor had LOD 0.65 ng/mL that was comparable to immunosensor LOD (0.36 ng/mL) in artificial CSF and showed its applicability in the clinical concentration range between 1 and 128 ng/mL.

多发性硬化症（MS）是一种反复发作的中枢神经系统（CNS）白质脱髓鞘炎症性疾病。多发性硬化症患者的人数在不断增加，但诊断过程仍然相当困难、昂贵，需要结合多种方法。髓鞘碱性蛋白（MBP）占中枢神经系统髓鞘的 30%。众所周知，MBP 释放到脑脊液（CSF）中可作为 MS 的生物指标。在此，早先为可能的治疗目的开发的髓鞘特异性 DNA 类似物和抗 MBP 抗体被用作生物受体，在相同的纳米改性传感器表面上识别 MBP，并比较了它们的性能。开发生物传感器的方法是将氧化石墨烯（GO）纳米颗粒集成到铅笔石墨电极（PGE）上，并固定生物受体分子以形成生物活性层，从而与 MBP 结合。测量采用电化学阻抗光谱（EIS）进行。使用人血清白蛋白（HSA）对生物传感器的选择性进行了评估。优化结合参数后，在人工 CSF 中对生物传感器进行了验证。结果表明，基于 LJM-5708 的适配传感器在人工 CSF 中的 LOD 为 0.65 纳克/毫升，与免疫传感器的 LOD（0.36 纳克/毫升）相当，表明其适用于 1 至 128 纳克/毫升的临床浓度范围。

{"title":"Demyelination detection in CSF based on electrochemical monitoring of myelin basic protein in comparison between Apta vs. Immuno sensing strategies","authors":"Marina Serin , Pınar Kara","doi":"10.1016/j.bioelechem.2024.108834","DOIUrl":"10.1016/j.bioelechem.2024.108834","url":null,"abstract":"<div><div>Multiple sclerosis (MS) is a recurrent inflammatory, demyelinating disease of the white matter in central nervous system (CNS). The number of MS patients is increasing, but the diagnostic process is still quite difficult, costly and requires combination of several methods. Myelin basic protein (MBP) makes up to 30 % of the myelin in CNS. It is known that MBP is released into the cerebrospinal fluid (CSF) as MS bioindicator. Herein, myelin specific DNA aptamer earlier developed for possible therapeutic purposes and anti-MBP antibody were applied as bioreceptors for MBP recognition on the same nanomodified sensor surfaces and their performances were compared. Biosensors were developed by using graphene oxide (GO) nanoparticles integrated onto pencil graphite electrodes (PGE) and bioreceptor molecules immobilized to create a bioactive layer for MBP binding. The measurements were run with electrochemical impedance spectroscopy (EIS). Selectivity of the biosensors was evaluated using human serum albumin (HSA). After optimization of binding parameters, biosensors were validated in artificial CSF. It was shown that LJM-5708 based aptasensor had LOD 0.65 ng/mL that was comparable to immunosensor LOD (0.36 ng/mL) in artificial CSF and showed its applicability in the clinical concentration range between 1 and 128 ng/mL.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108834"},"PeriodicalIF":4.8,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of endothelial nitric oxide synthase haplotypes on nitric oxide and peroxynitrite productions 内皮一氧化氮合酶单倍型对一氧化氮和过氧化亚硝酸盐生成的影响

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-10-16 DOI: 10.1016/j.bioelechem.2024.108837

Seham O. Alsulami , Tadeusz Malinski , Howard D. Dewald

The impact of four clinically significant genetic variants of endothelial nitric oxide synthase (eNOS) polymorphisms on the concentrations of nitric oxide [NO] and peroxynitrite [ONOO^–] has been given scant consideration. This study utilized a [NO]/[ONOO^–] ratio to determine the extent of endothelial dysfunction caused by these variations in the eNOS gene. The single nucleotide polymorphisms (T-786C, C-665T, and Glu298Asp) and a variable number of tandem repeats (intron 4 a/b/c) were genotyped in human umbilical vein endothelial cells (HUVEC), using sanger sequencing and DNA electrophoresis, respectively. Nanosensors were used to determine the maximal [NO] and [ONOO^–], while traditional and low-temperature SDS-PAGE were used to evaluate the expression of eNOS and the eNOS dimer-to-monomer ratio, respectively. The study results indicate that the eNOS haplotype H3 (G T/C C 4a/c allele) may have a protective effect against cardiovascular disease (CVD) with the [NO]/[ONOO^–] ratio higher than 2. However, the eNOS haplotypes H2 (G T/C C 4a/b) and H5 (T T/C C 4b) increase the susceptibility to CVD with [NO]/[ONOO^–] ratio lower than 1. The results suggest that certain eNOS genetic variants may influence susceptibility to cardiovascular disease (CVD) while other variants may have a protective effect.

内皮一氧化氮合酶（eNOS）多态性的四种具有临床意义的基因变异对一氧化氮[NO]和过氧化亚硝酸盐[ONOO-]浓度的影响一直鲜有研究。本研究利用[NO]/[ONOO-]比值来确定这些 eNOS 基因变异引起的内皮功能障碍的程度。利用桑格测序法和 DNA 电泳法分别对人脐静脉内皮细胞（HUVEC）中的单核苷酸多态性（T-786C、C-665T 和 Glu298Asp）和可变串联重复序列（内含子 4 a/b/c）进行了基因分型。纳米传感器用于测定最大[NO]和[ONOO-]，传统SDS-PAGE和低温SDS-PAGE分别用于评估eNOS的表达和eNOS二聚体与单体的比例。研究结果表明，当[NO]/[ONOO-]比值高于2时，eNOS单倍型H3（G T/C C 4a/c等位基因）可能对心血管疾病（CVD）有保护作用。研究结果表明，某些 eNOS 单倍型 H2（G T/C C 4a/b）和 H5（T T/C C 4b）可能会增加对[NO]/[ONOO-]比值低于 1 的心血管疾病的易感性。

{"title":"Influence of endothelial nitric oxide synthase haplotypes on nitric oxide and peroxynitrite productions","authors":"Seham O. Alsulami , Tadeusz Malinski , Howard D. Dewald","doi":"10.1016/j.bioelechem.2024.108837","DOIUrl":"10.1016/j.bioelechem.2024.108837","url":null,"abstract":"<div><div>The impact of four clinically significant genetic variants of endothelial nitric oxide synthase (eNOS) polymorphisms on the concentrations of nitric oxide [NO] and peroxynitrite [ONOO<sup>–</sup>] has been given scant consideration. This study utilized a [NO]/[ONOO<sup>–</sup>] ratio to determine the extent of endothelial dysfunction caused by these variations in the eNOS gene. The single nucleotide polymorphisms (T-786C, C-665T, and Glu298Asp) and a variable number of tandem repeats (intron 4 a/b/c) were genotyped in human umbilical vein endothelial cells (HUVEC), using sanger sequencing and DNA electrophoresis, respectively. Nanosensors were used to determine the maximal [NO] and [ONOO<sup>–</sup>], while traditional and low-temperature SDS-PAGE were used to evaluate the expression of eNOS and the eNOS dimer-to-monomer ratio, respectively. The study results indicate that the eNOS haplotype H3 (G T/C C 4a/c allele) may have a protective effect against cardiovascular disease (CVD) with the [NO]/[ONOO<sup>–</sup>] ratio higher than 2. However, the eNOS haplotypes H2 (G T/C C 4a/b) and H5 (T T/C C 4b) increase the susceptibility to CVD with [NO]/[ONOO<sup>–</sup>] ratio lower than 1. The results suggest that certain eNOS genetic variants may influence susceptibility to cardiovascular disease (CVD) while other variants may have a protective effect.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108837"},"PeriodicalIF":4.8,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sensitive electrochemical biosensor based on Pd@PdPtCo mesoporous nanopolyhedras as signal amplifiers for assay of cardiac troponin I 基于 Pd@PdPtCo 介孔纳米多面体作为信号放大器的灵敏电化学生物传感器，用于检测心肌肌钙蛋白 I。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-10-16 DOI: 10.1016/j.bioelechem.2024.108838

Miao Wang , He-Nan Sun , Xing-Yu Liu , Mingjun Liu , Shan-Shan Li

Cardiac troponin I (cTnI) has been widely used in clinical diagnosis of acute myocardial infarction (AMI). Herein, a sensitive electrochemical biosensor for cTnI analysis was designed, in which the simple synthesized Pd@PdPtCo mesoporous nanopolyhedras (MNPs) were utilized as signal amplifiers. The mesoporous polyhedral structure of Pd@PdPtCo MNPs endows them with more specific surface area and more active sites, as well as the synergistic effect between multiple metal elements, all of which increase the electrocatalytic performance of Pd@PdPtCo MNPs in efficiently oxidizing hydroquinone (HQ) to benzoquinone (BQ). Experimental results showed that Pd@PdPtCo MNPs had better performance in oxidation of HQ to BQ compared with their corresponding monometallic and bimetallic nanomaterials. With the aid of the interaction between antigens and antibodies, the peak current of HQ to BQ showed an upward trend with increasing concentration of cTnI, thus the quantitative detection of cTnI could be achieved. Under optimal conditions, the biosensor prepared in this work has a wider linear range (1.0 × 10⁻⁴–200 ng mL⁻¹) and a lower detection limit (0.031 pg mL⁻¹) than other sensors reported in literatures, coupled by good stability and high sensitivity. More importantly, it also performed well in complex serum environment, proving that the electrochemical sensor has a practical application potential in this field.

心肌肌钙蛋白 I（cTnI）已被广泛应用于急性心肌梗死（AMI）的临床诊断。本文设计了一种用于分析 cTnI 的灵敏电化学生物传感器，利用简单合成的 Pd@PdPtCo 介孔纳米多面体（MNPs）作为信号放大器。Pd@PdPtCo MNPs的介孔多面体结构使其具有更大的比表面积和更多的活性位点，以及多种金属元素之间的协同效应，所有这些都提高了Pd@PdPtCo MNPs高效氧化对苯二酚（HQ）为苯醌（BQ）的电催化性能。实验结果表明，与相应的单金属和双金属纳米材料相比，Pd@PdPtCo MNPs 在将对苯二酚（HQ）氧化为苯醌（BQ）的过程中具有更好的性能。借助抗原与抗体之间的相互作用，HQ 转化为 BQ 的峰值电流随 cTnI 浓度的增加而呈上升趋势，从而实现了 cTnI 的定量检测。在最佳条件下，与其他文献报道的传感器相比，本研究制备的生物传感器具有更宽的线性范围（1.0 × 10-4-200 ng mL-1）和更低的检测限（0.031 pg mL-1），同时还具有良好的稳定性和高灵敏度。更重要的是，它在复杂的血清环境中也有良好的表现，证明该电化学传感器在这一领域具有实际应用潜力。

{"title":"A sensitive electrochemical biosensor based on Pd@PdPtCo mesoporous nanopolyhedras as signal amplifiers for assay of cardiac troponin I","authors":"Miao Wang , He-Nan Sun , Xing-Yu Liu , Mingjun Liu , Shan-Shan Li","doi":"10.1016/j.bioelechem.2024.108838","DOIUrl":"10.1016/j.bioelechem.2024.108838","url":null,"abstract":"<div><div>Cardiac troponin I (cTnI) has been widely used in clinical diagnosis of acute myocardial infarction (AMI). Herein, a sensitive electrochemical biosensor for cTnI analysis was designed, in which the simple synthesized Pd@PdPtCo mesoporous nanopolyhedras (MNPs) were utilized as signal amplifiers. The mesoporous polyhedral structure of Pd@PdPtCo MNPs endows them with more specific surface area and more active sites, as well as the synergistic effect between multiple metal elements, all of which increase the electrocatalytic performance of Pd@PdPtCo MNPs in efficiently oxidizing hydroquinone (HQ) to benzoquinone (BQ). Experimental results showed that Pd@PdPtCo MNPs had better performance in oxidation of HQ to BQ compared with their corresponding monometallic and bimetallic nanomaterials. With the aid of the interaction between antigens and antibodies, the peak current of HQ to BQ showed an upward trend with increasing concentration of cTnI, thus the quantitative detection of cTnI could be achieved. Under optimal conditions, the biosensor prepared in this work has a wider linear range (1.0 × 10<sup>−4</sup>–200 ng mL<sup>−1</sup>) and a lower detection limit (0.031 pg mL<sup>−1</sup>) than other sensors reported in literatures, coupled by good stability and high sensitivity. More importantly, it also performed well in complex serum environment, proving that the electrochemical sensor has a practical application potential in this field.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108838"},"PeriodicalIF":4.8,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redox potential shapes spatial heterogeneity of mixed-cultured electroactive biofilm treating wastewater 氧化还原电位塑造了处理废水的混合培养电活性生物膜的空间异质性。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-10-15 DOI: 10.1016/j.bioelechem.2024.108836

Xian Wu , Guiqin Yang , Junhui Guo , Li Zhuang

The core of bioelectrochemical systems (BESs) is electrochemically active microorganisms (EAMs), which exert spatial heterogeneity on electrode surface and influences BESs performance. Setting an optimal potential is an effective strategy for improving and optimizing BESs performance, however, how the electrode potential affects spatial structure of microbial community within anode biofilm is not known. Using a complex substrate-fed BES with a wastewater inoculum, this study investigated the community structure and composition of the stratified biofilm developed under the potential of −0.3 V, 0 V, +0.3 V and +0.6 V (vs. saturated calomel electrode) by freezing microtome method and high-throughput sequencing analysis. The spatial heterogeneity of biofilm community was found to be dependent on the electrode potential and a less stratified community structure was observed for +0.6 V than other potentials. Within the biofilms, the inner layers selected more Geobacter and the outer layers enriched more Acinetobacter and Serratia, potentially suggested a stratification of electron transfer pathway and metabolite-based interspecies communications. The results demonstrated the response of spatial heterogeneity of anode biofilm community to the change of electrode potential, which helps to understand the selectivity and enrichment of kinetically efficient anodic microbiome by electron potential.

生物电化学系统（BES）的核心是电化学活性微生物（EAMs），它们对电极表面产生空间异质性并影响 BES 的性能。设置最佳电位是改善和优化 BES 性能的有效策略，但电极电位如何影响阳极生物膜内微生物群落的空间结构尚不清楚。本研究利用一种复杂的基质馈电 BES 和废水接种物，通过冷冻显微切片法和高通量测序分析，研究了在 -0.3V、0V、+0.3V 和 +0.6 V（相对于饱和甘汞电极）电位下形成的分层生物膜的群落结构和组成。研究发现，生物膜群落的空间异质性与电极电位有关，与其他电位相比，+0.6 V 的生物膜群落结构分层较少。在生物膜内，内层选择了更多的革兰氏菌，而外层则富集了更多的醋酐菌和沙雷氏菌，这可能表明了电子传递途径的分层和基于代谢物的种间通讯。研究结果表明了阳极生物膜群落的空间异质性对电极电位变化的响应，有助于理解电子电位对高效阳极微生物群落的选择性和富集性。

{"title":"Redox potential shapes spatial heterogeneity of mixed-cultured electroactive biofilm treating wastewater","authors":"Xian Wu , Guiqin Yang , Junhui Guo , Li Zhuang","doi":"10.1016/j.bioelechem.2024.108836","DOIUrl":"10.1016/j.bioelechem.2024.108836","url":null,"abstract":"<div><div>The core of bioelectrochemical systems (BESs) is electrochemically active microorganisms (EAMs), which exert spatial heterogeneity on electrode surface and influences BESs performance. Setting an optimal potential is an effective strategy for improving and optimizing BESs performance, however, how the electrode potential affects spatial structure of microbial community within anode biofilm is not known. Using a complex substrate-fed BES with a wastewater inoculum, this study investigated the community structure and composition of the stratified biofilm developed under the potential of −0.3 V, 0 V, +0.3 V and +0.6 V (vs. saturated calomel electrode) by freezing microtome method and high-throughput sequencing analysis. The spatial heterogeneity of biofilm community was found to be dependent on the electrode potential and a less stratified community structure was observed for +0.6 V than other potentials. Within the biofilms, the inner layers selected more <em>Geobacter</em> and the outer layers enriched more <em>Acinetobacter</em> and <em>Serratia</em>, potentially suggested a stratification of electron transfer pathway and metabolite-based interspecies communications. The results demonstrated the response of spatial heterogeneity of anode biofilm community to the change of electrode potential, which helps to understand the selectivity and enrichment of kinetically efficient anodic microbiome by electron potential.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108836"},"PeriodicalIF":4.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Gram-positive and Gram-negative bacteria membrane permeabilization induced by pulsed electric field using electrochemical admittance spectroscopy 利用电化学导纳光谱检测脉冲电场诱导的革兰氏阳性菌和革兰氏阴性菌膜渗透。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-10-11 DOI: 10.1016/j.bioelechem.2024.108835

Mindaugas Visockis, Paulius Ruzgys, Simona Gelažunaitė, Salvijus Vykertas, Saulius Šatkauskas

Electrochemical impedance or admittance spectroscopy (EIS or EAS) has been widely used for decades, offering a label-free, rapid, real-time, and non-destructive assay for optically opaque and turbid bacterial solutions. However, PEF-induced changes in the bacterial envelope can present challenges in detecting the extent of membrane permeabilization in both Gram-positive and Gram-negative bacteria due to their distinct morphological properties. Here, we used a new approach for detecting bacterial membrane permeabilization induced by PEF using electrochemical admittance spectroscopy (EAS). The metabolic activity results have shown that the larger L. d. bulgaricus bacteria was found to be significantly more resistant to PEF strengths ranging from 4 to 16 kV/cm than the smaller E. coli bacteria at shorter PEF treatment durations (10 × 10 µs pulses). Interestingly, the difference in the increase of the admittance magnitude and a decrease in phase angle between the PEF treatment times of 10 × 10 µs and 10 × 100 µs pulses at different PEF strengths was more pronounced for E. coli bacteria samples. Our results demonstrate that EAS is more effective in comparing the degree of membrane permeabilization of Gram-positive and Gram-negative bacteria when longer PEF treatment durations are applied.

几十年来，电化学阻抗或导纳光谱法（EIS 或 EAS）一直被广泛使用，它为光学不透明和浑浊的细菌溶液提供了一种无标记、快速、实时和非破坏性的检测方法。然而，由于革兰氏阳性菌和革兰氏阴性菌形态特性不同，PEF 诱导的细菌包膜变化会给检测它们的膜渗透程度带来挑战。在此，我们采用了一种新方法，利用电化学导纳光谱（EAS）检测 PEF 诱导的细菌膜渗透。代谢活动结果表明，在较短的 PEF 处理持续时间（10 × 10 µs 脉冲）下，较大的保加利亚乳杆菌对 4 至 16 kV/cm 的 PEF 强度的耐受性明显高于较小的大肠杆菌。有趣的是，在不同的 PEF 强度下，PEF 处理时间为 10 × 10 µs 和 10 × 100 µs 时，大肠杆菌样本的导纳幅度增加和相位角减小的差异更为明显。我们的研究结果表明，当采用较长的 PEF 处理时间时，EAS 能更有效地比较革兰氏阳性菌和革兰氏阴性菌的膜渗透程度。

{"title":"Detection of Gram-positive and Gram-negative bacteria membrane permeabilization induced by pulsed electric field using electrochemical admittance spectroscopy","authors":"Mindaugas Visockis, Paulius Ruzgys, Simona Gelažunaitė, Salvijus Vykertas, Saulius Šatkauskas","doi":"10.1016/j.bioelechem.2024.108835","DOIUrl":"10.1016/j.bioelechem.2024.108835","url":null,"abstract":"<div><div>Electrochemical impedance or admittance spectroscopy (EIS or EAS) has been widely used for decades, offering a label-free, rapid, real-time, and non-destructive assay for optically opaque and turbid bacterial solutions. However, PEF-induced changes in the bacterial envelope can present challenges in detecting the extent of membrane permeabilization in both Gram-positive and Gram-negative bacteria due to their distinct morphological properties. Here, we used a new approach for detecting bacterial membrane permeabilization induced by PEF using electrochemical admittance spectroscopy (EAS). The metabolic activity results have shown that the larger <em>L. d. bulgaricus</em> bacteria was found to be significantly more resistant to PEF strengths ranging from 4 to 16 kV/cm than the smaller <em>E. coli</em> bacteria at shorter PEF treatment durations (10 × 10 µs pulses). Interestingly, the difference in the increase of the admittance magnitude and a decrease in phase angle between the PEF treatment times of 10 × 10 µs and 10 × 100 µs pulses at different PEF strengths was more pronounced for <em>E. coli</em> bacteria samples. Our results demonstrate that EAS is more effective in comparing the degree of membrane permeabilization of Gram-positive and Gram-negative bacteria when longer PEF treatment durations are applied.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108835"},"PeriodicalIF":4.8,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical response mechanism of DNA damaged cells: DNA damage repair and purine metabolism activation DNA 损伤细胞的电化学反应机制：DNA 损伤修复和嘌呤代谢激活

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-10-09 DOI: 10.1016/j.bioelechem.2024.108832

Shulan Qi , Jiaqi Fu , Yue Li , Chaoqun Fei , Jiahuan Zhang , Liyuan Sui , Shi Zhou , Jinlian Li , Yanli Zhao , Dongmei Wu

In modern society, due to the sharp increase in pollutants that cause DNA damage, there is a growing demand for innovative detection techniques and biomarkers. In this paper, the electrochemical behavior of HepG2 cells exposed to CdCl₂ was investigated, and the electrochemical response mechanism of DNA damage was identified by exploring the correlation between the DNA damage response and purine metabolism. Western blot analysis revealed that the expression levels of ATM and Ku70 increased at 0.3 μM CdCl₂, indicating a DNA damage response and activation of DNA repair processes. Simultaneously, elevated expression levels of PRPP aminotransferase, HPRT, and XOD were observed, leading to an increase in intracellular purine levels and electrochemical signals. The expression of Ku70 peaked at 0.5 μM CdCl₂, indicating the highest DNA repair activity. The expression profiles of these purine metabolism proteins mirrored those of Ku70, suggesting a strong correlation between the activation of purine metabolism and DNA damage repair. Consistently, intracellular purine levels exhibited a similar trend, leading to corresponding changes in electrochemical signals. In summary, electrochemical using intracellular purines as biomarkers has the potential to emerge as a novel method for detecting early DNA damage.

在现代社会，由于导致DNA损伤的污染物急剧增加，人们对创新检测技术和生物标志物的需求与日俱增。本文研究了暴露于 CdCl2 的 HepG2 细胞的电化学行为，并通过探索 DNA 损伤反应与嘌呤代谢之间的相关性，确定了 DNA 损伤的电化学反应机制。Western 印迹分析显示，在 0.3 μM CdCl2 条件下，ATM 和 Ku70 的表达水平升高，表明存在 DNA 损伤应答并激活了 DNA 修复过程。同时，观察到 PRPP 转氨酶、HPRT 和 XOD 的表达水平升高，导致细胞内嘌呤水平和电化学信号增加。Ku70 的表达在 0.5 μM CdCl2 时达到峰值，表明其 DNA 修复活性最高。这些嘌呤代谢蛋白的表达谱与 Ku70 的表达谱相似，表明嘌呤代谢的激活与 DNA 损伤修复之间存在密切联系。同样，细胞内的嘌呤水平也呈现出类似的趋势，从而导致电化学信号发生相应的变化。总之，利用细胞内嘌呤作为生物标记物的电化学方法有可能成为检测早期 DNA 损伤的一种新方法。

{"title":"Electrochemical response mechanism of DNA damaged cells: DNA damage repair and purine metabolism activation","authors":"Shulan Qi , Jiaqi Fu , Yue Li , Chaoqun Fei , Jiahuan Zhang , Liyuan Sui , Shi Zhou , Jinlian Li , Yanli Zhao , Dongmei Wu","doi":"10.1016/j.bioelechem.2024.108832","DOIUrl":"10.1016/j.bioelechem.2024.108832","url":null,"abstract":"<div><div>In modern society, due to the sharp increase in pollutants that cause DNA damage, there is a growing demand for innovative detection techniques and biomarkers. In this paper, the electrochemical behavior of HepG2 cells exposed to CdCl<sub>2</sub> was investigated, and the electrochemical response mechanism of DNA damage was identified by exploring the correlation between the DNA damage response and purine metabolism. Western blot analysis revealed that the expression levels of ATM and Ku70 increased at 0.3 μM CdCl<sub>2</sub>, indicating a DNA damage response and activation of DNA repair processes. Simultaneously, elevated expression levels of PRPP aminotransferase, HPRT, and XOD were observed, leading to an increase in intracellular purine levels and electrochemical signals. The expression of Ku70 peaked at 0.5 μM CdCl<sub>2</sub>, indicating the highest DNA repair activity. The expression profiles of these purine metabolism proteins mirrored those of Ku70, suggesting a strong correlation between the activation of purine metabolism and DNA damage repair. Consistently, intracellular purine levels exhibited a similar trend, leading to corresponding changes in electrochemical signals. In summary, electrochemical using intracellular purines as biomarkers has the potential to emerge as a novel method for detecting early DNA damage.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108832"},"PeriodicalIF":4.8,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical/fluorescent dual-mode aptasensor based on 3D porous AuNPs/MXene for detection of ultra-trace mercury (Hg2+) 基于三维多孔 AuNPs/MXene 的电化学/荧光双模式灵敏传感器，用于检测超痕量汞 (Hg2+)。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-10-05 DOI: 10.1016/j.bioelechem.2024.108833

Luo Li , Xiaoyi Yan , Yu Liu , Yue Xing , Puyu Zhao , Yunxue Zhu , Ning Liu , Kaiping Sun , Zhiquan Zhang , Shengyong Zhai

In this work, the dual-mode aptasensor based on 3D porous AuNPs/MXene using “turn-on” electrochemical method and “turn-off” fluorescent strategy was fabricated. Here, 2D MXene was processed into 3D porous MXene by sacrificial polymethylmethacrylate (PMMA) spherical template. And the meteor hammer-like AuNPs which had good electrochemical properties and quenching effect on fluorescence was synthesized by single electrodeposition. Dual-signal labeled Nile Blue (NB) was in situ grafted to the Hg²⁺ aptamer ends of 3D porous AuNPs/MXene/GCE, and an efficient and sensitive signal interface was constructed to realize the sensitive detection of Hg²⁺. 3D porous AuNPs/MXene had the advantages of large specific surface area, excellent electron transmission performance and signal amplification. The experimental results indicated that this sensor exhibited high sensitivity to Hg²⁺ in both electrochemical and fluorescent sensing, with detection limits of 2.69 fM and 1.60 fM, respectively. Further, the dual-mode aptasensor can ensure the detection accuracy and target quantization. The dual-mode aptasensor has been successfully applied to the ultra-trace detection of Hg²⁺ in actual water samples, which shows the potential of aptamer sensor in detecting heavy metal ions in environmental monitoring.

本研究采用 "开启 "电化学方法和 "关闭 "荧光策略，制备了基于三维多孔 AuNPs/MXene 的双模式灵敏传感器。在这里，二维 MXene 通过牺牲聚甲基丙烯酸甲酯（PMMA）球形模板加工成三维多孔 MXene。通过单次电沉积合成了具有良好电化学性能和荧光淬灭效果的流星锤状 AuNPs。将双信号标记的尼罗蓝（NB）原位接枝到三维多孔 AuNPs/MXene/GCE 的 Hg2+ 通感末端，构建了高效灵敏的信号接口，实现了对 Hg2+ 的灵敏检测。三维多孔 AuNPs/MXene/GCE 具有比表面积大、电子传输性能好和信号放大等优点。实验结果表明，该传感器在电化学传感和荧光传感中均表现出对 Hg2+ 的高灵敏度，检测限分别为 2.69 fM 和 1.60 fM。此外，该双模式传感器还能确保检测精度和目标量化。该双模适配体传感器已成功应用于实际水样中 Hg2+ 的超痕量检测，显示了适配体传感器在环境监测中检测重金属离子的潜力。

{"title":"Electrochemical/fluorescent dual-mode aptasensor based on 3D porous AuNPs/MXene for detection of ultra-trace mercury (Hg2+)","authors":"Luo Li , Xiaoyi Yan , Yu Liu , Yue Xing , Puyu Zhao , Yunxue Zhu , Ning Liu , Kaiping Sun , Zhiquan Zhang , Shengyong Zhai","doi":"10.1016/j.bioelechem.2024.108833","DOIUrl":"10.1016/j.bioelechem.2024.108833","url":null,"abstract":"<div><div>In this work, the dual-mode aptasensor based on 3D porous AuNPs/MXene using “turn-on” electrochemical method and “turn-off” fluorescent strategy was fabricated. Here, 2D MXene was processed into 3D porous MXene by sacrificial polymethylmethacrylate (PMMA) spherical template. And the meteor hammer-like AuNPs which had good electrochemical properties and quenching effect on fluorescence was synthesized by single electrodeposition. Dual-signal labeled Nile Blue (NB) was in situ grafted to the Hg<sup>2+</sup> aptamer ends of 3D porous AuNPs/MXene/GCE, and an efficient and sensitive signal interface was constructed to realize the sensitive detection of Hg<sup>2+</sup>. 3D porous AuNPs/MXene had the advantages of large specific surface area, excellent electron transmission performance and signal amplification. The experimental results indicated that this sensor exhibited high sensitivity to Hg<sup>2+</sup> in both electrochemical and fluorescent sensing, with detection limits of 2.69 fM and 1.60 fM, respectively. Further, the dual-mode aptasensor can ensure the detection accuracy and target quantization. The dual-mode aptasensor has been successfully applied to the ultra-trace detection of Hg<sup>2+</sup> in actual water samples, which shows the potential of aptamer sensor in detecting heavy metal ions in environmental monitoring.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108833"},"PeriodicalIF":4.8,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitigation of biocorrosion of X80 carbon steel by a shale microbiome biofilm using a green biocide enhanced by d-amino acids 使用由 d-氨基酸增强的绿色杀菌剂，通过页岩微生物群生物膜缓解 X80 碳钢的生物腐蚀。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-09-30 DOI: 10.1016/j.bioelechem.2024.108831

Di Wang , Shengbei Wen , Hanjun Liu , Pan Liu , Jiahao Xiong , Yalin Wu , Zhilin Li , Zehong Tian , Bei Liu , Dake Xu , Tingyue Gu , Fuhui Wang

Microbiologically influenced corrosion (MIC) in shale gas field is a major threat with the hydraulic fracturing fluid injected into the subsurface. In this study, the microbiome collected from a shale gas produced water sample was extracted and cultivated in ATCC 1249 medium modified with 10 g/L NaCl anaerobically at 30 °C. d-amino acids, which were reported as biocide enhancers, were found to enhance 2,2-dibromo-3-nitrilopropionamide (DBNPA) biocide on the mitigation of shale microbiome MIC on X80 carbon steel. The combination of 50 ppm (w/w) d-leucine + 50 ppm d-alanine + 1 ppm d-tyrosine had the best enhancement effect on 50 ppm DBNPA with 84 % less weight loss, and 67 % lower corrosion current density (i_corr) compared to 50 ppm DBNPA alone. The corrosion data were consistent with the enhanced biofilm inhibition observation. The experimental data also indicated that d-tyrosine used alone at a low dosage of 1 ppm enhanced DBNPA considerably, with 44 % less weight loss and 47 % less i_corr. The electrochemical results showed the positive response of shale gas microbiome biofilm to the injected magnetite nanoparticles indicating the extracellular electron transfer might be a main mechanism for its corrosion.

页岩气田中的微生物腐蚀（MIC）是注入地下的水力压裂液的一个主要威胁。在这项研究中，从页岩气产水样中提取了微生物组，并在 ATCC 1249 培养基中用 10 g/L NaCl 在 30 °C 下厌氧培殖。50 ppm（w/w）d-亮氨酸 + 50 ppm d-丙氨酸 + 1 ppm d-酪氨酸的组合对 50 ppm DBNPA 的增强效果最好，与单独使用 50 ppm DBNPA 相比，重量损失减少了 84%，腐蚀电流密度（icorr）降低了 67%。腐蚀数据与增强的生物膜抑制观察结果一致。实验数据还表明，以 1 ppm 的低剂量单独使用 d- 酪氨酸可显著增强 DBNPA 的效果，重量损失减少 44%，icorr 减少 47%。电化学结果表明，页岩气微生物群生物膜对注入的磁铁矿纳米粒子有积极反应，这表明胞外电子转移可能是其腐蚀的主要机制。

{"title":"Mitigation of biocorrosion of X80 carbon steel by a shale microbiome biofilm using a green biocide enhanced by d-amino acids","authors":"Di Wang , Shengbei Wen , Hanjun Liu , Pan Liu , Jiahao Xiong , Yalin Wu , Zhilin Li , Zehong Tian , Bei Liu , Dake Xu , Tingyue Gu , Fuhui Wang","doi":"10.1016/j.bioelechem.2024.108831","DOIUrl":"10.1016/j.bioelechem.2024.108831","url":null,"abstract":"<div><div>Microbiologically influenced corrosion (MIC) in shale gas field is a major threat with the hydraulic fracturing fluid injected into the subsurface. In this study, the microbiome collected from a shale gas produced water sample was extracted and cultivated in ATCC 1249 medium modified with 10 g/L NaCl anaerobically at 30 °C. <span>d</span>-amino acids, which were reported as biocide enhancers, were found to enhance 2,2-dibromo-3-nitrilopropionamide (DBNPA) biocide on the mitigation of shale microbiome MIC on X80 carbon steel. The combination of 50 ppm (w/w) <span>d</span>-leucine + 50 ppm <span>d</span>-alanine + 1 ppm <span>d</span>-tyrosine had the best enhancement effect on 50 ppm DBNPA with 84 % less weight loss, and 67 % lower corrosion current density (<em>i</em><sub>corr</sub>) compared to 50 ppm DBNPA alone. The corrosion data were consistent with the enhanced biofilm inhibition observation. The experimental data also indicated that <span>d</span>-tyrosine used alone at a low dosage of 1 ppm enhanced DBNPA considerably, with 44 % less weight loss and 47 % less <em>i</em><sub>corr</sub>. The electrochemical results showed the positive response of shale gas microbiome biofilm to the injected magnetite nanoparticles indicating the extracellular electron transfer might be a main mechanism for its corrosion.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108831"},"PeriodicalIF":4.8,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inside out: Exploring edible biocatalytic biosensors for health monitoring 由内而外：探索用于健康监测的可食用生物催化生物传感器。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-09-27 DOI: 10.1016/j.bioelechem.2024.108830

Verdiana Marchianò , Angelo Tricase , Alessandra Cimino , Blanca Cassano , Michele Catacchio , Eleonora Macchia , Luisa Torsi , Paolo Bollella

Edible biosensors can measure a wide range of physiological and biochemical parameters, including temperature, pH, gases, gastrointestinal biomarkers, enzymes, hormones, glucose, and drug levels, providing real-time data. Edible biocatalytic biosensors represent a new frontier within healthcare technology available for remote medical diagnosis. The main challenges to develop edible biosensors are: i) finding edible materials (i.e. redox mediators, conductive materials, binders and biorecognition elements such as enzymes) complying with Food and Drug Administration (FDA), European Food Safety Authority (EFSA) and European Medicines Agency (EMEA) regulations; ii) developing bioelectronics able to operate in extreme working conditions such as low pH (∼pH 1.5 gastric fluids etc.), body temperature (between 37 °C and 40 °C) and highly viscous bodily fluids that may cause surface biofouling issues. Nowadays, advanced printing techniques can revolutionize the design and manufacturing of edible biocatalytic biosensors.

This review outlines recent research on biomaterials suitable for creating edible biocatalytic biosensors, focusing on their electrochemical properties such as electrical conductivity and redox potential. It also examines biomaterials as substrates for printing and discusses various printing methods, highlighting challenges and perspectives for edible biocatalytic biosensors.

可食用生物传感器可测量各种生理和生化参数，包括温度、pH 值、气体、胃肠道生物标志物、酶、激素、葡萄糖和药物水平，并提供实时数据。可食用生物催化生物传感器代表了医疗保健技术的一个新领域，可用于远程医疗诊断。开发可食用生物传感器的主要挑战是：i) 寻找符合食品和药物管理局（FDA）、欧洲食品安全局（EFSA）和欧洲药品管理局（EMEA）规定的可食用材料（即氧化还原介质、导电材料、粘合剂和生物识别元素，如酶）；ii) 开发能够在极端工作条件下运行的生物电子器件，如低 pH 值（pH 1.5 胃液等）、体温（37 °C至40 °C）和高粘度体液等极端工作条件下工作的生物电子器件，这些条件可能会导致表面生物污染问题。如今，先进的打印技术可以彻底改变可食用生物催化生物传感器的设计和制造。本综述概述了有关适合制造可食用生物催化生物传感器的生物材料的最新研究，重点关注其电化学特性，如导电性和氧化还原电位。它还研究了作为印刷基底的生物材料，并讨论了各种印刷方法，突出强调了可食用生物催化生物传感器所面临的挑战和前景。

{"title":"Inside out: Exploring edible biocatalytic biosensors for health monitoring","authors":"Verdiana Marchianò , Angelo Tricase , Alessandra Cimino , Blanca Cassano , Michele Catacchio , Eleonora Macchia , Luisa Torsi , Paolo Bollella","doi":"10.1016/j.bioelechem.2024.108830","DOIUrl":"10.1016/j.bioelechem.2024.108830","url":null,"abstract":"<div><div>Edible biosensors can measure a wide range of physiological and biochemical parameters, including temperature, pH, gases, gastrointestinal biomarkers, enzymes, hormones, glucose, and drug levels, providing real-time data. Edible biocatalytic biosensors represent a new frontier within healthcare technology available for remote medical diagnosis. The main challenges to develop edible biosensors are: <em>i)</em> finding edible materials (i.e. redox mediators, conductive materials, binders and biorecognition elements such as enzymes) complying with Food and Drug Administration (FDA), European Food Safety Authority (EFSA) and European Medicines Agency (EMEA) regulations; ii) developing bioelectronics able to operate in extreme working conditions such as low pH (∼pH 1.5 gastric fluids etc.), body temperature (between 37 °C and 40 °C) and highly viscous bodily fluids that may cause surface biofouling issues. Nowadays, advanced printing techniques can revolutionize the design and manufacturing of edible biocatalytic biosensors.</div><div>This review outlines recent research on biomaterials suitable for creating edible biocatalytic biosensors, focusing on their electrochemical properties such as electrical conductivity and redox potential. It also examines biomaterials as substrates for printing and discusses various printing methods, highlighting challenges and perspectives for edible biocatalytic biosensors.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108830"},"PeriodicalIF":4.8,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0