Pub Date : 2024-09-22DOI: 10.1016/j.bioelechem.2024.108829
Qiu-Ren Pan, Ying-Qi Ouyang, Hui-Huan Jiang, Dong-Ni Ou, Jun-Ying Zhong, Nan Li
The rational development of high-performance anode and cathode electrodes for microbial fuel cells (MFCs) is crucial for enhancing MFC performance. However, complex synthesis methods and single-performance electrode materials hinder their large-scale implementation. Here, three-dimensional hierarchical porous (3DHP) Fe3O4/Fe-N-C composites were prepared via the hard template method. Notably, Fe3O4/Fe-N-C-0.04-600 demonstrated uniformly dispersed Fe3O4 nanoparticles and abundant Fe-Nx and pyridinic nitrogen, showing excellent catalytic performance for oxygen reduction reaction (ORR) with a half-wave potential (E1/2) of 0.74 V (vs. RHE), surpassing Pt/C (0.66 V vs. RHE). Moreover, Fe3O4/Fe-N-C-0.04-600 demonstrated favorable biocompatibility as an anode material, enhancing anode biomass and extracellular electron transfer efficiency. Sequencing results confirmed its promotion of electrophilic microorganisms in the anode biofilm. MFCs employing Fe3O4/Fe-N-C-0.04-600 as both anode and cathode materials achieved a maximum power density of 831.8 ± 27.7 mW m−2, enduring operation for 38 days. This study presents a novel approach for rational MFC design, emphasizing bifunctional materials capable of serving as anode materials for microorganism growth and as cathode catalysts for ORR catalysis.
{"title":"Bifunctional electrode materials: Enhancing microbial fuel cell efficiency with 3D hierarchical porous Fe3O4/Fe-N-C structures","authors":"Qiu-Ren Pan, Ying-Qi Ouyang, Hui-Huan Jiang, Dong-Ni Ou, Jun-Ying Zhong, Nan Li","doi":"10.1016/j.bioelechem.2024.108829","DOIUrl":"10.1016/j.bioelechem.2024.108829","url":null,"abstract":"<div><div>The rational development of high-performance anode and cathode electrodes for microbial fuel cells (MFCs) is crucial for enhancing MFC performance. However, complex synthesis methods and single-performance electrode materials hinder their large-scale implementation. Here, three-dimensional hierarchical porous (3DHP) Fe<sub>3</sub>O<sub>4</sub>/Fe-N-C composites were prepared via the hard template method. Notably, Fe<sub>3</sub>O<sub>4</sub>/Fe-N-C-0.04-600 demonstrated uniformly dispersed Fe<sub>3</sub>O<sub>4</sub> nanoparticles and abundant Fe-N<sub>x</sub> and pyridinic nitrogen, showing excellent catalytic performance for oxygen reduction reaction (ORR) with a half-wave potential (E<sub>1/2</sub>) of 0.74 V (vs. RHE), surpassing Pt/C (0.66 V vs. RHE). Moreover, Fe<sub>3</sub>O<sub>4</sub>/Fe-N-C-0.04-600 demonstrated favorable biocompatibility as an anode material, enhancing anode biomass and extracellular electron transfer efficiency. Sequencing results confirmed its promotion of electrophilic microorganisms in the anode biofilm. MFCs employing Fe<sub>3</sub>O<sub>4</sub>/Fe-N-C-0.04-600 as both anode and cathode materials achieved a maximum power density of 831.8 ± 27.7 mW m<sup>−2</sup>, enduring operation for 38 days. This study presents a novel approach for rational MFC design, emphasizing bifunctional materials capable of serving as anode materials for microorganism growth and as cathode catalysts for ORR catalysis.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108829"},"PeriodicalIF":4.8,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142319741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-21DOI: 10.1016/j.bioelechem.2024.108824
Kamarajan Rajagopalan , Jackson Durairaj Selvan Christyraj , Nivetha Balamurugan , Johnson Retnaraj Samuel Selvan Christyraj , Vipin Mohan Dan , Periyasamy Radhakrishnan , Ashwin Barath Vaidhyalingham , Hari Prasath Nagaiah
Electric stimulation regulates many cellular processes like cell proliferation, differentiation, apoptosis and cellular migration. Despite its crucial role in regulating stem cells and regeneration, it remains underexplored in both in-vivo and in-vitro settings. In this study, Eudrilus eugeniae are subjected to electric stimulation (1.5 V) prior and after amputation and which augments regeneration up to double-time. Blocking epimorphosis using 2 M thymidine retracts regeneration kinetics to one-third but such inhibition was rescued by applying electric stimulation which propels an overactive morphallaxis pattern of regeneration. Excreting electric stimulation on control worms shows minimal impact, whereas it enhances the key regenerative proteins like VEGF, COX2, YAP, c-Myc, and Wnt3a on amputated worms. Upon blocking epimorphosis, all these key regenerative proteins are down-regulated but through electric stimulation, the cells are reprogrammed to express a triple fold of the mentioned regenerative proteins, that further promotes morphallaxis. In 3T3 cells, electric stimulation accelerates cell proliferation and migrations in 5 secs exposure and it exerts its function by overexpressing VEGF mediated by MEK1. Wnt3a expression was gradually upregulated in increasing exposure (5 and 25 secs) which aids in maintaining the stemness property. The molecular mechanism underlying regeneration capability can assist in designing novel therapeutic applications.
{"title":"Low-energy electric shock ameliorates cell proliferation, morphallaxis, and regeneration via driving key regenerative proteins in earthworm and 3T3 cells","authors":"Kamarajan Rajagopalan , Jackson Durairaj Selvan Christyraj , Nivetha Balamurugan , Johnson Retnaraj Samuel Selvan Christyraj , Vipin Mohan Dan , Periyasamy Radhakrishnan , Ashwin Barath Vaidhyalingham , Hari Prasath Nagaiah","doi":"10.1016/j.bioelechem.2024.108824","DOIUrl":"10.1016/j.bioelechem.2024.108824","url":null,"abstract":"<div><div>Electric stimulation regulates many cellular processes like cell proliferation, differentiation, apoptosis and cellular migration. Despite its crucial role in regulating stem cells and regeneration, it remains underexplored in both in-vivo and in-vitro settings. In this study, <em>Eudrilus eugeniae</em> are subjected to electric stimulation (1.5 V) prior and after amputation and which augments regeneration up to double-time. Blocking epimorphosis using 2 M thymidine retracts regeneration kinetics to one-third but such inhibition was rescued by applying electric stimulation which propels an overactive morphallaxis pattern of regeneration. Excreting electric stimulation on control worms shows minimal impact, whereas it enhances the key regenerative proteins like VEGF, COX2, YAP, c-Myc, and Wnt3a on amputated worms. Upon blocking epimorphosis, all these key regenerative proteins are down-regulated but through electric stimulation, the cells are reprogrammed to express a triple fold of the mentioned regenerative proteins, that further promotes morphallaxis. In 3T3 cells, electric stimulation accelerates cell proliferation and migrations in 5 secs exposure and it exerts its function by overexpressing VEGF mediated by MEK1. Wnt3a expression was gradually upregulated in increasing exposure (5 and 25 secs) which aids in maintaining the stemness property. The molecular mechanism underlying regeneration capability can assist in designing novel therapeutic applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108824"},"PeriodicalIF":4.8,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142319742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-21DOI: 10.1016/j.bioelechem.2024.108823
Hamidreza Ghaedamini, Dong-Shik Kim
This study presents the development of a novel non-enzymatic electrochemical biosensor for the real-time detection of hydrogen peroxide (H2O2) based on a composite of cerium metal–organic frameworks (Ce-MOFs), hemin, and graphene oxide (GO). The Ce-MOFs served as an efficient matrix for hemin encapsulation, while GO enhanced the conductivity of the composite. Characterization techniques including scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, UV–vis spectroscopy, and thermogravimetric analysis (TGA) confirmed the successful integration of hemin into the Ce-MOFs. The Ce-MOFs@hemin/GO-modified sensor demonstrated sensitive H2O2 detection due to the exceptional electrocatalytic activity of Ce-MOFs@hemin and the high conductivity of GO. This biosensor exhibited a linear response to H2O2 concentrations from 0.05 to 10 mM with a limit of detection (LOD) of 9.3 μM. The capability of the biosensor to detect H2O2 released from human prostate carcinoma cells was demonstrated, highlighting its potential for real-time monitoring of cellular oxidative stress in complex biological environments. To further assess its practical applicability, the sensor was tested in human serum samples, yielding promising results with recovery values ranging from 94.50 % to 103.29 %. In addition, the sensor showed excellent selectivity against common interfering compounds due to the outstanding peroxidase-like activity of the composite.
{"title":"A non-enzymatic hydrogen peroxide biosensor based on cerium metal-organic frameworks, hemin, and graphene oxide composite","authors":"Hamidreza Ghaedamini, Dong-Shik Kim","doi":"10.1016/j.bioelechem.2024.108823","DOIUrl":"10.1016/j.bioelechem.2024.108823","url":null,"abstract":"<div><div>This study presents the development of a novel non-enzymatic electrochemical biosensor for the real-time detection of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) based on a composite of cerium metal–organic frameworks (Ce-MOFs), hemin, and graphene oxide (GO). The Ce-MOFs served as an efficient matrix for hemin encapsulation, while GO enhanced the conductivity of the composite. Characterization techniques including scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, UV–vis spectroscopy, and thermogravimetric analysis (TGA) confirmed the successful integration of hemin into the Ce-MOFs. The Ce-MOFs@hemin/GO-modified sensor demonstrated sensitive H<sub>2</sub>O<sub>2</sub> detection due to the exceptional electrocatalytic activity of Ce-MOFs@hemin and the high conductivity of GO. This biosensor exhibited a linear response to H<sub>2</sub>O<sub>2</sub> concentrations from 0.05 to 10 mM with a limit of detection (LOD) of 9.3 μM. The capability of the biosensor to detect H<sub>2</sub>O<sub>2</sub> released from human prostate carcinoma cells was demonstrated, highlighting its potential for real-time monitoring of cellular oxidative stress in complex biological environments. To further assess its practical applicability, the sensor was tested in human serum samples, yielding promising results with recovery values ranging from 94.50 % to 103.29 %. In addition, the sensor showed excellent selectivity against common interfering compounds due to the outstanding peroxidase-like activity of the composite.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108823"},"PeriodicalIF":4.8,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-21DOI: 10.1016/j.bioelechem.2024.108822
Min Wang, Mingzhe Jiang, Wenjing Lai, Zihan Yan, Tingting Wang, Yu Qi, Chenglin Hong
α-Fetoprotein (AFP) is widely recognized as an important marker for monitoring hepatocellular carcinoma (HCC), and its monitoring using two different transduction mechanisms is an effective way to avoid the risk of false positives or false negatives. In this paper, Au@Cu/Cu2O-rGO was used as a photothermal converter as well as an actuator to promote the decomposition of hydrogen peroxide (H2O2), which was further designed as a probe for dual-mode detection to quantitatively assess AFP. The composite nanomaterials possessed photothermal conversion efficiencies (η) of up to 54.9 % and catalytically generated signals up to 1.6 times greater, relative to a single material. Based on the generated temperature and current signals, AFP has been sensitively detected in the range of 0.01–100 ng/mL, with limits of detection (LOD) of 5.62 pg/mL and 1.23 pg/mL, respectively. The dual-mode assay combines portability with high accuracy for the detection of human health systems.
{"title":"Dual-mode detection of α-fetoprotein using the photothermal effect and peroxidase-like activity of Au@Cu/Cu2O-rGO","authors":"Min Wang, Mingzhe Jiang, Wenjing Lai, Zihan Yan, Tingting Wang, Yu Qi, Chenglin Hong","doi":"10.1016/j.bioelechem.2024.108822","DOIUrl":"10.1016/j.bioelechem.2024.108822","url":null,"abstract":"<div><div>α-Fetoprotein (AFP) is widely recognized as an important marker for monitoring hepatocellular carcinoma (HCC), and its monitoring using two different transduction mechanisms is an effective way to avoid the risk of false positives or false negatives. In this paper, Au@Cu/Cu<sub>2</sub>O-rGO was used as a photothermal converter as well as an actuator to promote the decomposition of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), which was further designed as a probe for dual-mode detection to quantitatively assess AFP. The composite nanomaterials possessed photothermal conversion efficiencies (η) of up to 54.9 % and catalytically generated signals up to 1.6 times greater, relative to a single material. Based on the generated temperature and current signals, AFP has been sensitively detected in the range of 0.01–100 ng/mL, with limits of detection (LOD) of 5.62 pg/mL and 1.23 pg/mL, respectively. The dual-mode assay combines portability with high accuracy for the detection of human health systems.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108822"},"PeriodicalIF":4.8,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142319740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.bioelechem.2024.108826
Luís C. Almeida , Jorge F. Zeferino , Clara Branco , Guiseppe Squillaci , Alessandra Morana , Romana Santos , Petri Ihalainen , Liji Sobhana , Jorge P. Correia , Ana S. Viana
The successful fabrication of biosensors is greatly limited by the immobilization of their bioreceptor, thus we propose a facile and reproducible two-step method to modify graphite electrodes with a bacterial laccase, relying on a fast and controllable potentiostatic process to coat graphite surfaces with biomolecule-compatible thin films of polynorepinephrine (ePNE) and polydopamine (ePDA). Both polymers, synthesized with a similar thickness, were functionalized with bacterial laccase, displaying distinct electrochemical transducing behaviours at pH 5.0 and 7.0. ePNE layer enables adequate electron transfer of anionic and cationic species in acidic and neutral media, whereas transduction across ePDA strongly depends on pH and redox probe charge. ePNE stands out by improving the amperometric responses of the biosensing interface towards a phenolic acid (gallic acid) and a flavonoid (catechin), in respect to ePDA. The optimal graphite/ePNE/laccase interface outperforms biosensing interfaces based on fungal laccases at neutral pH, displaying detection sensitivities of 104 and 14.4 µA cm−2 mM−1 for gallic acid and catechin, respectively. The fine synthetic control of the ePNE bio-inspired transduction layer and the use of an alkaliphilic bacterial laccase enabled the construction of an amperometric biosensing interface with extended pH range of polyphenols detection present in food products and agro-industrial waste.
{"title":"Polynorepinephrine and polydopamine-bacterial laccase coatings for phenolic amperometric biosensors","authors":"Luís C. Almeida , Jorge F. Zeferino , Clara Branco , Guiseppe Squillaci , Alessandra Morana , Romana Santos , Petri Ihalainen , Liji Sobhana , Jorge P. Correia , Ana S. Viana","doi":"10.1016/j.bioelechem.2024.108826","DOIUrl":"10.1016/j.bioelechem.2024.108826","url":null,"abstract":"<div><div>The successful fabrication of biosensors is greatly limited by the immobilization of their bioreceptor, thus we propose a facile and reproducible two-step method to modify graphite electrodes with a bacterial laccase, relying on a fast and controllable potentiostatic process to coat graphite surfaces with biomolecule-compatible thin films of polynorepinephrine (ePNE) and polydopamine (ePDA). Both polymers, synthesized with a similar thickness, were functionalized with bacterial laccase, displaying distinct electrochemical transducing behaviours at pH 5.0 and 7.0. ePNE layer enables adequate electron transfer of anionic and cationic species in acidic and neutral media, whereas transduction across ePDA strongly depends on pH and redox probe charge. ePNE stands out by improving the amperometric responses of the biosensing interface towards a phenolic acid (gallic acid) and a flavonoid (catechin), in respect to ePDA. The optimal graphite/ePNE/laccase interface outperforms biosensing interfaces based on fungal laccases at neutral pH, displaying detection sensitivities of 104 and 14.4 µA cm<sup>−2</sup> mM<sup>−1</sup> <!-->for gallic acid and catechin, respectively. The fine synthetic control of the ePNE bio-inspired transduction layer and the use of an alkaliphilic bacterial laccase enabled the construction of an amperometric biosensing interface with extended pH range of polyphenols detection present in food products and agro-industrial waste.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108826"},"PeriodicalIF":4.8,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001889/pdfft?md5=862478700bbd86aee15c05b010a77322&pid=1-s2.0-S1567539424001889-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.bioelechem.2024.108828
Ying Li , Shiang Wang , Gaiping Li , Chi Zhang , Lina Zou
In this study, a signal-on photoelectrochemical (PEC) aptasensor for the ultrasensitive determination of kanamycin (KANA) was constructed using WO3/CdS heterojunction as photoactive material. Firstly, WO3/CdS heterojunction with excellent photoelectric response was successfully prepared by simple co-precipitation method, resulting in a strong and stable initial photocurrent. In addition, amino modified aptamers were immobilized on the electrode surface by glutaraldehyde as biological recognition components. In the presence of the target KANA, it is specifically recognized and captured by the aptamers. More importantly, KANA can act as a signal amplifier to enhance the photocurrent due to the oxidation of KANA by photogenerated holes. Therefore, a signal-on PEC aptasensor based on WO3/CdS heterojunction with high selectivity was obtained for the detection of KANA. Under optimized experimental conditions, the PEC aptasensor demonstrated a wide linear range of 10 pM to 400 nM, with a detection limit of 6.77 pM. Meanwhile, the designed PEC aptasensor had been successfully utilized for the analytical examination of milk, fish, serum, and water samples.
{"title":"A signal-on photoelectrochemical aptasensor based on WO3/CdS heterojunction for the ultrasensitive detection of kanamycin","authors":"Ying Li , Shiang Wang , Gaiping Li , Chi Zhang , Lina Zou","doi":"10.1016/j.bioelechem.2024.108828","DOIUrl":"10.1016/j.bioelechem.2024.108828","url":null,"abstract":"<div><div>In this study, a signal-on photoelectrochemical (PEC) aptasensor for the ultrasensitive determination of kanamycin (KANA) was constructed using WO<sub>3</sub>/CdS heterojunction as photoactive material. Firstly, WO<sub>3</sub>/CdS heterojunction with excellent photoelectric response was successfully prepared by simple co-precipitation method, resulting in a strong and stable initial photocurrent. In addition, amino modified aptamers were immobilized on the electrode surface by glutaraldehyde as biological recognition components. In the presence of the target KANA, it is specifically recognized and captured by the aptamers. More importantly, KANA can act as a signal amplifier to enhance the photocurrent due to the oxidation of KANA by photogenerated holes. Therefore, a signal-on PEC aptasensor based on WO<sub>3</sub>/CdS heterojunction with high selectivity was obtained for the detection of KANA. Under optimized experimental conditions, the PEC aptasensor demonstrated a wide linear range of 10 pM to 400 nM, with a detection limit of 6.77 pM. Meanwhile, the designed PEC aptasensor had been successfully utilized for the analytical examination of milk, fish, serum, and water samples.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108828"},"PeriodicalIF":4.8,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001907/pdfft?md5=2a8b0c46c2a381bf4dafb87327270136&pid=1-s2.0-S1567539424001907-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.bioelechem.2024.108827
Yu-Jie Chen , Yu-Ying Li , Bao-Lin Xiao , Lin-Lin Ma , Ke-Xin Xu , Sanad Abdalbage Mohammed Abdalsadeg , Tao Hong , Ali Akbar Moosavi-Movahedi , Reza Yousefi , Yan-Na Ning , Jun Hong
Constructing a biosensor to detect luteolin content accurately is essential, especially considering its specific health benefits at certain concentrations. In this work, the reaction of HRP catalyzed luteolin could be successfully applied in electrocatalytic processes, the oxidation process of electron loss and dehydrogenation occurring on the electrode replaced the hydrogen receptor role of H2O2 in the HRP biocatalytic process. This oxidation reaction had an apparent current response, thus achieving accurate measurement of luteolin. On this biosensor, CTAB was used to disperse MWCNTs, and BSA was used to improve the hydrophobicity of MWCNTs, which was conducive to the subsequent AuNPs fixation of HRP. Three detection methods (LSV, DPV and SWV) for the detection of luteolin were compared and showed that SWV method had a wider linear range (1 × 10−8–2 × 10−5 M) and lower detection limit (8 × 10−10 M). The determination of luteolin in Traditional Chinese Medicine (TCM) by high performance liquid chromatography (HPLC) and biosensor was almost identical. Therefore, this biosensor could successfully replace HPLC in detecting luteolin in TCM.
{"title":"Electrochemical biosensor based on functional nanomaterials and horseradish peroxidase for the determination of luteolin in peanut shell, honeysuckle and perilla","authors":"Yu-Jie Chen , Yu-Ying Li , Bao-Lin Xiao , Lin-Lin Ma , Ke-Xin Xu , Sanad Abdalbage Mohammed Abdalsadeg , Tao Hong , Ali Akbar Moosavi-Movahedi , Reza Yousefi , Yan-Na Ning , Jun Hong","doi":"10.1016/j.bioelechem.2024.108827","DOIUrl":"10.1016/j.bioelechem.2024.108827","url":null,"abstract":"<div><div>Constructing a biosensor to detect luteolin content accurately is essential, especially considering its specific health benefits at certain concentrations. In this work, the reaction of HRP catalyzed luteolin could be successfully applied in electrocatalytic processes, the oxidation process of electron loss and dehydrogenation occurring on the electrode replaced the hydrogen receptor role of H<sub>2</sub>O<sub>2</sub> in the HRP biocatalytic process. This oxidation reaction had an apparent current response, thus achieving accurate measurement of luteolin. On this biosensor, CTAB was used to disperse MWCNTs, and BSA was used to improve the hydrophobicity of MWCNTs, which was conducive to the subsequent AuNPs fixation of HRP. Three detection methods (LSV, DPV and SWV) for the detection of luteolin were compared and showed that SWV method had a wider linear range (1 × 10<sup>−8</sup>–2 × 10<sup>−5</sup> M) and lower detection limit (8 × 10<sup>−10</sup> M). The determination of luteolin in Traditional Chinese Medicine (TCM) by high performance liquid chromatography (HPLC) and biosensor was almost identical. Therefore, this biosensor could successfully replace HPLC in detecting luteolin in TCM.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108827"},"PeriodicalIF":4.8,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001890/pdfft?md5=faf1ddc86a7e01abbc3f8226967d9fab&pid=1-s2.0-S1567539424001890-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-19DOI: 10.1016/j.bioelechem.2024.108825
Marcin Szalkowski , Małgorzata Kiliszek , Ersan Harputlu , Miriam Izzo , C. Gokhan Unlu , Sebastian Mackowski , Kasim Ocakoglu , Joanna Kargul , Dorota Kowalska
We present the novel design of photosystem I (PSI)-based biosolar cell, whereby conductive transparent electrode materials, such as ITO or FTO, are replaced with glass covered with silver island film. This nanostructured metallic layer combines high electric conductance with enhancing the absorption efficiency of the PSI biocatalyst via the plasmonic effect. We demonstrate strong enhancement of the photocurrent generated in the biohybrid electrode composed of oriented layers of PSI reaction centers due to plasmonic interactions of the PSI fluorophores and redox centres with the conductive silver island film.
{"title":"Bimodal functionality of highly conductive nanostructured silver film towards improved performance of photosystem I-based graphene photocathode","authors":"Marcin Szalkowski , Małgorzata Kiliszek , Ersan Harputlu , Miriam Izzo , C. Gokhan Unlu , Sebastian Mackowski , Kasim Ocakoglu , Joanna Kargul , Dorota Kowalska","doi":"10.1016/j.bioelechem.2024.108825","DOIUrl":"10.1016/j.bioelechem.2024.108825","url":null,"abstract":"<div><div>We present the novel design of photosystem I (PSI)-based biosolar cell, whereby conductive transparent electrode materials, such as ITO or FTO, are replaced with glass covered with silver island film. This nanostructured metallic layer combines high electric conductance with enhancing the absorption efficiency of the PSI biocatalyst via the plasmonic effect. We demonstrate strong enhancement of the photocurrent generated in the biohybrid electrode composed of oriented layers of PSI reaction centers due to plasmonic interactions of the PSI fluorophores and redox centres with the conductive silver island film.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108825"},"PeriodicalIF":4.8,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-18DOI: 10.1016/j.bioelechem.2024.108819
Xin Li , Daobin Han , Xinmin Li , Chunjie Zhou , Bo Shen , Honglu Wei , Qian Lou , Changjin Liu , Tingmei Chen
The construction of simple, stable, low-cost and reproducible enzyme-free electrochemical biosensors can effectively avoid the problem of signal attenuation caused by enzyme inactivation. Hererin, we prepared a novel nanoenzymes PdPtCu mesoporous nanocubes (MNCs) to construct a label-free sandwich electrochemical immunosensor for the highly sensitivity detection of HIV-p24. PdPtCu MNCs have excellent peroxidase activity against hydrogen peroxide (H2O2) due to their synergistic ternary composition, large surface area and ability to penetrate mesoporous channels. Moreover, highly conductive and biocompatible gold nanoparticles@graphene oxide (AuNPs@GO) was introduced as a substrate to modify a glassy carbon electrode (GCE). Owing to the excellent electrochemical performance of the PdPtCu MNCs and AuNPs@GO, the developed immunosensors exhibited a good linear response from 0.04 pg/mL to 100 ng/mL with a low detection limit of 20 fg/mL. In addition, the established method exhibited excellent practical performance in human serum. This novel strategy provides a promising platform for ultrasensitive detection of the HIV-p24 in the field of clinical diagnostics.
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Pub Date : 2024-09-17DOI: 10.1016/j.bioelechem.2024.108821
Théo Le Berre , Julien Marchalot , Marie Frénéa-Robin , Jérôme Cros , Frédéric Prat , Guilhem Rival
The dielectric properties of pancreatic tissues from human healthy and tumour-bearing tissues have been extracted from impedance measurement on ex vivo, freshly excised samples. They are compared to pig pancreas samples, measured following the same protocol. The purpose is to add data to the scarce literature on the properties of the human pancreas and pancreatic tumours, for treatment planning, tissue identification and numerical simulations. The conductivity measured at 500 kHz for human healthy pancreas is 0.26 S/m, while the conductivity of tumour-bearing tissues is 0.44 S/m. Those values differ significantly from that listed in the IT IS database at 0.57 S/m, suggesting an update might be to consider. However, measures of relative permittivity are in accordance with the database with a value of approximately 2.3x103. Ex vivo porcine model, while being less conductive than human pancreas with 0.16 S/m at the same frequency, is deemed a relevant model when studying pancreatic applications of electromagnetic fields-based treatments, such as radiofrequency ablation.
通过对新鲜切除的体外样本进行阻抗测量，提取了人体健康胰腺组织和肿瘤胰腺组织的介电特性。它们与按照相同方案测量的猪胰腺样本进行了比较。目的是为有关人体胰腺和胰腺肿瘤特性的稀缺文献增添数据，以用于治疗规划、组织识别和数值模拟。人体健康胰腺在 500 kHz 频率下测得的电导率为 0.26 S/m，而肿瘤组织的电导率为 0.44 S/m。这些数值与 IT IS 数据库中列出的 0.57 S/m 有很大差异，表明可能需要考虑更新。不过，相对介电常数的测量值与数据库一致，约为 2.3x103。猪体内外模型在相同频率下的导电性低于 0.16 S/m 的人体胰腺，但在研究基于电磁场的胰腺应用治疗（如射频消融）时，猪体内外模型被认为是一个相关模型。
{"title":"Measurement and comparison of dielectric properties of human pancreatic tumours, healthy tissues and porcine tissues ex vivo between 1Hz and 1MHz","authors":"Théo Le Berre , Julien Marchalot , Marie Frénéa-Robin , Jérôme Cros , Frédéric Prat , Guilhem Rival","doi":"10.1016/j.bioelechem.2024.108821","DOIUrl":"10.1016/j.bioelechem.2024.108821","url":null,"abstract":"<div><p>The dielectric properties of pancreatic tissues from human healthy and tumour-bearing tissues have been extracted from impedance measurement on <em>ex vivo</em>, freshly excised samples. They are compared to pig pancreas samples, measured following the same protocol. The purpose is to add data to the scarce literature on the properties of the human pancreas and pancreatic tumours, for treatment planning, tissue identification and numerical simulations. The conductivity measured at 500 kHz for human healthy pancreas is 0.26 S/m, while the conductivity of tumour-bearing tissues is 0.44 S/m. Those values differ significantly from that listed in the IT IS database at 0.57 S/m, suggesting an update might be to consider. However, measures of relative permittivity are in accordance with the database with a value of approximately 2.3x10<sup>3</sup>. <em>Ex vivo</em> porcine model, while being less conductive than human pancreas with 0.16 S/m at the same frequency, is deemed a relevant model when studying pancreatic applications of electromagnetic fields-based treatments, such as radiofrequency ablation.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108821"},"PeriodicalIF":4.8,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S156753942400183X/pdfft?md5=923a6632fbe84dbd4e3e70d111d5b20d&pid=1-s2.0-S156753942400183X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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