Bioelectrochemistry最新文献

英文中文

Non-invasive alcohol biosensor based on gold nanoparticles and carbon nanotubes network for dynamic monitoring of sweat alcohol

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-02-13 DOI: 10.1016/j.bioelechem.2025.108943

Wenjing Peng , Shan Li , Hui Gao , Mengjie Su , Yaqiu Zhou , Zhengyuan Ding , Qiyu Jiang , Chunmei Yu

Intemperance can lead to health issues or other potential harms to society. Consequently, accurate detection of alcohol concentration in human fluid is an essential and challenging task. In this paper, we reported an efficient and reliable method for highly sensitive and selective monitoring of alcohol in sweat. This stretchable alcohol biosensor has been fabricated by transferring multi-walled carbon nanotubes (MWCNTs) film on polydimethylsiloxane (PDMS) substrate followed by immobilization of Au nanoparticles (AuNPs) and alcohol oxidase enzyme (AOx). The biosensor possesses satisfactory mechanical stability, including excellent resistance to stretching, bending and twisting. Sandwich structure formed on the electrode surface by MWCNTs and AuNPs provides excellent electrical conductivity and electrochemical performance for biosensors. The biosensor exhibited a wide linear range from 1.5 μM to 30 mM toward alcohol and the detection limit was 0.5 μM. Furthermore, owing to the specificity of the AOx, the biosensor displayed splendid selectivity. The real sample tests show that the constructed biosensor has the ability to monitor sweat alcohol, and the results were consistent with those obtained by gas chromatography. This research offers a versatile method for the development of flexible electrochemical biosensors, which has promising applications in noninvasive and accurate detection of alcohol in human sweat.

{"title":"Non-invasive alcohol biosensor based on gold nanoparticles and carbon nanotubes network for dynamic monitoring of sweat alcohol","authors":"Wenjing Peng , Shan Li , Hui Gao , Mengjie Su , Yaqiu Zhou , Zhengyuan Ding , Qiyu Jiang , Chunmei Yu","doi":"10.1016/j.bioelechem.2025.108943","DOIUrl":"10.1016/j.bioelechem.2025.108943","url":null,"abstract":"<div><div>Intemperance can lead to health issues or other potential harms to society. Consequently, accurate detection of alcohol concentration in human fluid is an essential and challenging task. In this paper, we reported an efficient and reliable method for highly sensitive and selective monitoring of alcohol in sweat. This stretchable alcohol biosensor has been fabricated by transferring multi-walled carbon nanotubes (MWCNTs) film on polydimethylsiloxane (PDMS) substrate followed by immobilization of Au nanoparticles (AuNPs) and alcohol oxidase enzyme (AOx). The biosensor possesses satisfactory mechanical stability, including excellent resistance to stretching, bending and twisting. Sandwich structure formed on the electrode surface by MWCNTs and AuNPs provides excellent electrical conductivity and electrochemical performance for biosensors. The biosensor exhibited a wide linear range from 1.5 μM to 30 mM toward alcohol and the detection limit was 0.5 μM. Furthermore, owing to the specificity of the AOx, the biosensor displayed splendid selectivity. The real sample tests show that the constructed biosensor has the ability to monitor sweat alcohol, and the results were consistent with those obtained by gas chromatography. This research offers a versatile method for the development of flexible electrochemical biosensors, which has promising applications in noninvasive and accurate detection of alcohol in human sweat.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108943"},"PeriodicalIF":4.8,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bystander effect of metal byproducts released from electroporated cells after electroporation in vitro

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-02-12 DOI: 10.1016/j.bioelechem.2025.108940

Alenka Maček Lebar , Tjaša Potočnik , Janez Ščančar , Stefan Marković , Tamara Polajžer

Electrodes dissolution during electroporation releases metal ions into the medium, altering the microenvironment of electroporated cells and allowing metal ions to penetrate cell membrane. During cell membrane repair, homeostasis restoration or activation of cell death pathways, cells eliminate excess metals from the cytoplasm and membrane. This study assessed the effects of post-electroporation metal byproducts on untreated (non-electroporated) cells in vitro.

CHO and HCT116 cells were electroporated with three pulse protocols (unipolar: 100 μs, 5 ms; bipolar: 2 μs) using either aluminum or stainless-steel electrodes. After electroporation, cells were transferred to fresh growth medium and incubated for 2 or 4 h. Incubation period allowed either cell recovery or the activation of cell death pathways, leading to the accumulation of metal byproducts in the incubation medium.

Stainless-steel electrodes with the 5 ms pulse protocol caused a considerable increase in iron, chromium and nickel ions in incubation medium compared to aluminum electrodes or other protocols. Metal ions in incubation medium caused toxicity in non-electroporated cells, disrupting cell cycle function or inducing cell death. The observed toxicity results from combined effects of metal ions on cellular functions and the mechanisms the cells use to protect themselves from metal overload.

{"title":"Bystander effect of metal byproducts released from electroporated cells after electroporation in vitro","authors":"Alenka Maček Lebar , Tjaša Potočnik , Janez Ščančar , Stefan Marković , Tamara Polajžer","doi":"10.1016/j.bioelechem.2025.108940","DOIUrl":"10.1016/j.bioelechem.2025.108940","url":null,"abstract":"<div><div>Electrodes dissolution during electroporation releases metal ions into the medium, altering the microenvironment of electroporated cells and allowing metal ions to penetrate cell membrane. During cell membrane repair, homeostasis restoration or activation of cell death pathways, cells eliminate excess metals from the cytoplasm and membrane. This study assessed the effects of post-electroporation metal byproducts on untreated (non-electroporated) cells <em>in vitro</em>.</div><div>CHO and HCT116 cells were electroporated with three pulse protocols (unipolar: 100 μs, 5 ms; bipolar: 2 μs) using either aluminum or stainless-steel electrodes. After electroporation, cells were transferred to fresh growth medium and incubated for 2 or 4 h. Incubation period allowed either cell recovery or the activation of cell death pathways, leading to the accumulation of metal byproducts in the incubation medium.</div><div>Stainless-steel electrodes with the 5 ms pulse protocol caused a considerable increase in iron, chromium and nickel ions in incubation medium compared to aluminum electrodes or other protocols. Metal ions in incubation medium caused toxicity in non-electroporated cells, disrupting cell cycle function or inducing cell death. The observed toxicity results from combined effects of metal ions on cellular functions and the mechanisms the cells use to protect themselves from metal overload.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108940"},"PeriodicalIF":4.8,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrodeposition of carbon nanotubes and conjugation of arginyl-glycyl-aspartic acid for the following of glioblastoma cells on bionanocomposites

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-02-07 DOI: 10.1016/j.bioelechem.2025.108937

Belguzar Karadag, Simge Er Zeybekler, Sultan Sacide Gelen, Leila Sabour-Takanlou, Maryam Sabour-Takanlou, Cigir Biray Avci, Dilek Odaci

The improvement of surface treatment methods that permit the tuning of cell adhesion on the surface of biomaterials and devices is of considerable importance. Here, multi-walled carbon nanotubes (MWCNT) were modified with 4-aminothiophenol (4ATP). Then, electrodeposition of MWCNT-4ATP was carried out on 4ATP-modified screen-printed gold electrodes (SP-Au). After conjugation of Arginyl-glycyl-aspartic acid (RGD)-peptide on Poly(MWCNT-4ATP), the adhesion of U-87MG glioblastoma cells was examined by differential pulse voltammetry (DPV) technique. The synthesized MWCNT-4ATP and the obtained Poly(MWCNT-4ATP)/RGD surfaces were characterized using Scanning Electron Microscopy-Energy Dispersive X-Ray Spectrometer (SEM-EDS), Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR), X-Ray Photoelectron Spectrometer (XPS). The linear range for U-87MG glioblastoma cells was 10²–10⁶ cells/mL. The developed Poly(MWCNT-4ATP)/RGD cell adhesion platform provided monitoring of U-87MG glioblastoma cells using DPV technique and fluorescent imaging.

{"title":"Electrodeposition of carbon nanotubes and conjugation of arginyl-glycyl-aspartic acid for the following of glioblastoma cells on bionanocomposites","authors":"Belguzar Karadag, Simge Er Zeybekler, Sultan Sacide Gelen, Leila Sabour-Takanlou, Maryam Sabour-Takanlou, Cigir Biray Avci, Dilek Odaci","doi":"10.1016/j.bioelechem.2025.108937","DOIUrl":"10.1016/j.bioelechem.2025.108937","url":null,"abstract":"<div><div>The improvement of surface treatment methods that permit the tuning of cell adhesion on the surface of biomaterials and devices is of considerable importance. Here, multi-walled carbon nanotubes (MWCNT) were modified with 4-aminothiophenol (4ATP). Then, electrodeposition of MWCNT-4ATP was carried out on 4ATP-modified screen-printed gold electrodes (SP-Au). After conjugation of Arginyl-glycyl-aspartic acid <strong>(</strong>RGD)-peptide on Poly(MWCNT-4ATP), the adhesion of U-87MG glioblastoma cells was examined by differential pulse voltammetry (DPV) technique. The synthesized MWCNT-4ATP and the obtained Poly(MWCNT-4ATP)/RGD surfaces were characterized using Scanning Electron Microscopy-Energy Dispersive X-Ray Spectrometer (SEM-EDS), Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR), X-Ray Photoelectron Spectrometer (XPS). The linear range for U-87MG glioblastoma cells was 10<sup>2</sup>–10<sup>6</sup> cells/mL. The developed Poly(MWCNT-4ATP)/RGD cell adhesion platform provided monitoring of U-87MG glioblastoma cells using DPV technique and fluorescent imaging.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108937"},"PeriodicalIF":4.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Numerical modeling of giant pore formation in vesicles under msPEF-induced electroporation: Role of charging time and waveform

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-02-07 DOI: 10.1016/j.bioelechem.2025.108926

Nalinikanta Behera, Rochish M. Thaokar

Giant unilamellar vesicle is the closest prototypical model for investigating membrane electrodeformation and electroporation in biological cells. This work employs numerical simulations to investigate the effect of membrane charging time on vesicle electroporation under milli-second pulsed-electric-field (msPEF) of different waveforms. Our numerical approach, which implements the effect of electric stretching on membrane tension and precise calculation of pore energy, successfully predicts the formation of giant pores of

O (1)

μ m

size as observed in previous experiments. The poration zone is found to extend up to certain angles as measured from the poles, termed critical angles. An increase in charging time delays pore formation, decreases the pore density, and trims down the poration zone. Counterintuitively, this effect promotes significant pore growth. Moreover, there exists a cut-off charging time above which pore formation is completely inhibited. This phenomenon is particularly pronounced with square bipolar pulses. Comparisons with the previous experimental results reveal that electrodeformation-poration-induced membrane surface area variation and that induced only by electroporation evolves in a similar fashion. Therefore, although the agreements are qualitative, the present electroporation model can be used as the simplest tool to predict the evolution of vesicles under electric pulses in laboratory experiments.

{"title":"Numerical modeling of giant pore formation in vesicles under msPEF-induced electroporation: Role of charging time and waveform","authors":"Nalinikanta Behera, Rochish M. Thaokar","doi":"10.1016/j.bioelechem.2025.108926","DOIUrl":"10.1016/j.bioelechem.2025.108926","url":null,"abstract":"<div><div>Giant unilamellar vesicle is the closest prototypical model for investigating membrane electrodeformation and electroporation in biological cells. This work employs numerical simulations to investigate the effect of membrane charging time on vesicle electroporation under milli-second pulsed-electric-field (msPEF) of different waveforms. Our numerical approach, which implements the effect of electric stretching on membrane tension and precise calculation of pore energy, successfully predicts the formation of giant pores of <span><math><mrow><mi>O</mi><mrow><mo>(</mo><mn>1</mn><mo>)</mo></mrow></mrow></math></span> <span><math><mrow><mi>μ</mi><mi>m</mi></mrow></math></span> size as observed in previous experiments. The poration zone is found to extend up to certain angles as measured from the poles, termed critical angles. An increase in charging time delays pore formation, decreases the pore density, and trims down the poration zone. Counterintuitively, this effect promotes significant pore growth. Moreover, there exists a cut-off charging time above which pore formation is completely inhibited. This phenomenon is particularly pronounced with square bipolar pulses. Comparisons with the previous experimental results reveal that electrodeformation-poration-induced membrane surface area variation and that induced only by electroporation evolves in a similar fashion. Therefore, although the agreements are qualitative, the present electroporation model can be used as the simplest tool to predict the evolution of vesicles under electric pulses in laboratory experiments.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108926"},"PeriodicalIF":4.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid bacterial detection through microfluidic integration with a glucometer

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-02-06 DOI: 10.1016/j.bioelechem.2025.108936

Merve Eryilmaz , Sibel Ilbasmis-Tamer , Sallahuddin Panhwar , Emine Kübra Tayyarcan , İsmail Hakkı Boyaci , Zekiye Suludere , Demet Çetin , Adem Zengin , Ender Yıldırım , Uğur Tamer

We present a novel approach for sensitive and portable detection of pathogenic bacteria, which is crucial for household and clinical practice. Our method employs immunoliposomes, antibodies, and microchip to detect specific pathogens quantitatively. Gold and metal metal–organic nanoparticles and liposomes were characterized using high-resolution techniques like TEM and SEM. Utilizing a commercial, personal glucose meter (PGM), we initially detected released glucose from antibody-modified liposomes and microchips with MOF-NPs. Detection on the microchip was achieved within 30 min, while the PGM analysis took only one minute for targeted bacteria, yielding glucose signals of 66 mg/dL and 69 mg/dL, respectively. Serial dilutions with group A-Streptococcus pyogenes (GAS) (1.4 × 10^{^4}–1.4 × 10^{^8} CFU/mL) demonstrated quantitative measurement applicability. This innovative approach and a portable PGM hold promise for various industries, including physician labs, hospitals, and households.

{"title":"Rapid bacterial detection through microfluidic integration with a glucometer","authors":"Merve Eryilmaz , Sibel Ilbasmis-Tamer , Sallahuddin Panhwar , Emine Kübra Tayyarcan , İsmail Hakkı Boyaci , Zekiye Suludere , Demet Çetin , Adem Zengin , Ender Yıldırım , Uğur Tamer","doi":"10.1016/j.bioelechem.2025.108936","DOIUrl":"10.1016/j.bioelechem.2025.108936","url":null,"abstract":"<div><div>We present a novel approach for sensitive and portable detection of pathogenic bacteria, which is crucial for household and clinical practice. Our method employs immunoliposomes, antibodies, and microchip to detect specific pathogens quantitatively. Gold and metal metal–organic nanoparticles and liposomes were characterized using high-resolution techniques like TEM and SEM. Utilizing a commercial, personal glucose meter (PGM), we initially detected released glucose from antibody-modified liposomes and microchips with MOF-NPs. Detection on the microchip was achieved within 30 min, while the PGM analysis took only one minute for targeted bacteria, yielding glucose signals of 66 mg/dL and 69 mg/dL, respectively. Serial dilutions with group A-<em>Streptococcus pyogenes</em> (GAS) (1.4 × 10<sup>^4</sup>–1.4 × 10<sup>^8</sup> CFU/mL) demonstrated quantitative measurement applicability. This innovative approach and a portable PGM hold promise for various industries, including physician labs, hospitals, and households.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108936"},"PeriodicalIF":4.8,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of pulsed electric field (PEF) as a strategy to enhance aminoglycosides efficacy against Gram-negative bacteria

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-02-05 DOI: 10.1016/j.bioelechem.2025.108935

Mindaugas Visockis, Paulius Ruzgys, Simona Gelažunaitė, Salvijus Vykertas, Saulius Šatkauskas

In this study, two aminoglycosides (AGs), Kanamycin and Gentamicin, with similar modes of action and molecular weights, were combined with PEF treatment to enhance the inactivation of E. coli cells. Various PEF strengths were applied to assess the combined effect. To compare the inactivation efficacy of different AGs, bacterial growth measurements in suspension were performed at 3 and 10 h intervals over a 10-h period after PEF treatment. Interestingly, it was found that the additive effect of PEF treatment on E. coli growth inhibition was significantly greater with Kanamycin (IC₅₀) than with Gentamicin (IC₅₀). Further analysis revealed that the combined treatment with Kanamycin (IC₅₀) was most effective within a timeframe of around 3 h. Our findings suggest that PEF treatment can significantly enhance the efficacy of AGs against Gram-negative bacteria; however, the extent of the additive effect varies depending on the specific antibiotic and the intensity of the applied PEF treatment.

{"title":"Application of pulsed electric field (PEF) as a strategy to enhance aminoglycosides efficacy against Gram-negative bacteria","authors":"Mindaugas Visockis, Paulius Ruzgys, Simona Gelažunaitė, Salvijus Vykertas, Saulius Šatkauskas","doi":"10.1016/j.bioelechem.2025.108935","DOIUrl":"10.1016/j.bioelechem.2025.108935","url":null,"abstract":"<div><div>In this study, two aminoglycosides (AGs), Kanamycin and Gentamicin, with similar modes of action and molecular weights, were combined with PEF treatment to enhance the inactivation of <em>E. coli</em> cells. Various PEF strengths were applied to assess the combined effect. To compare the inactivation efficacy of different AGs, bacterial growth measurements in suspension were performed at 3 and 10 h intervals over a 10-h period after PEF treatment. Interestingly, it was found that the additive effect of PEF treatment on <em>E. coli</em> growth inhibition was significantly greater with Kanamycin (IC<sub>50</sub>) than with Gentamicin (IC<sub>50</sub>). Further analysis revealed that the combined treatment with Kanamycin (IC<sub>50</sub>) was most effective within a timeframe of around 3 h. Our findings suggest that PEF treatment can significantly enhance the efficacy of AGs against Gram-negative bacteria; however, the extent of the additive effect varies depending on the specific antibiotic and the intensity of the applied PEF treatment.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108935"},"PeriodicalIF":4.8,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143376932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A microfluidic co-culture platform for lung cancer cells electrotaxis study under the existence of stromal cells

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-02-03 DOI: 10.1016/j.bioelechem.2025.108917

Lina Zheng, Keying Li, Xianmei Tang, Cuiping Li, Hailiang Nie, Lirong Han, Yaping Li

Tumor metastasis is an important reason for the poor prognosis and high mortality in cancer patients. As major component of stromal cells in tumor microenvironment, cancer-associated fibroblasts (CAFs) secreted various factors to promote tumor metastasis. Studies have indicated that endogenous direct current electric field (dcEF) around tumor tissue induced directional migration of cancer cells. However, the regulatory effect of CAFs on cancer migration under dcEF stimulation is still unknown. In this study, a two-layers polydimethylsiloxane (PDMS)-based microfluidic chip was fabricated. The introduction of concave structures achieved the non-contacted co-culture of different cell types, and parallel channels in the chip provided stable and homogeneous dcEF. Cells electrotactic response was evaluated under co-culture circumstance. The results showed that CAFs exhibited directional migration towards anode under dcEF stimulation, while A549 cells had a trend of directional migration towards cathode. The co-existence of CAFs and dcEF significantly enhanced the motility and cathodal migration of A549 cells, suggesting synergistic influences of chemotaxis from CAFs and electrotaxis from dcEF stimulation. Moreover, we demonstrated that lung normal fibroblasts acquired CAFs properties after stimulated by dcEF, characterizing by increasing gene expression of α-SMA and IL-6. Overall, Our device and study provide new insight for tumor electrotaxis in complex microenvironment.

{"title":"A microfluidic co-culture platform for lung cancer cells electrotaxis study under the existence of stromal cells","authors":"Lina Zheng, Keying Li, Xianmei Tang, Cuiping Li, Hailiang Nie, Lirong Han, Yaping Li","doi":"10.1016/j.bioelechem.2025.108917","DOIUrl":"10.1016/j.bioelechem.2025.108917","url":null,"abstract":"<div><div>Tumor metastasis is an important reason for the poor prognosis and high mortality in cancer patients. As major component of stromal cells in tumor microenvironment, cancer-associated fibroblasts (CAFs) secreted various factors to promote tumor metastasis. Studies have indicated that endogenous direct current electric field (dcEF) around tumor tissue induced directional migration of cancer cells. However, the regulatory effect of CAFs on cancer migration under dcEF stimulation is still unknown. In this study, a two-layers polydimethylsiloxane (PDMS)-based microfluidic chip was fabricated. The introduction of concave structures achieved the non-contacted co-culture of different cell types, and parallel channels in the chip provided stable and homogeneous dcEF. Cells electrotactic response was evaluated under co-culture circumstance. The results showed that CAFs exhibited directional migration towards anode under dcEF stimulation, while A549 cells had a trend of directional migration towards cathode. The co-existence of CAFs and dcEF significantly enhanced the motility and cathodal migration of A549 cells, suggesting synergistic influences of chemotaxis from CAFs and electrotaxis from dcEF stimulation. Moreover, we demonstrated that lung normal fibroblasts acquired CAFs properties after stimulated by dcEF, characterizing by increasing gene expression of α-SMA and IL-6. Overall, Our device and study provide new insight for tumor electrotaxis in complex microenvironment.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108917"},"PeriodicalIF":4.8,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143155968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Molecular monolayers on silicon as substrates for biosensors” [Bioelectrochem. 80(1) (2010) 17–25] 硅上的分子单层作为生物传感器的基底"[Bioelectrochem.

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-02-01 DOI: 10.1016/j.bioelechem.2024.108849

L. Touahir , P. Allongue , D. Aureau , R. Boukherroub , J.-N. Chazalviel , E. Galopin , A.C. Gouget-Laemmel , C. Henry de Villeneuve , A. Moraillon , J. Niedziółka-Jönsson , F. Ozanam , J. Salvador Andresa , S. Sam , I. Solomon , S. Szunerits

引用次数: 0

Emerging screening platform characterises aminoquinoline structure–activity relationships with phospholipid layers

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-02-01 DOI: 10.1016/j.bioelechem.2025.108927

Bethany Crow , Roland Grafstrom , Vesa Hongisto , Mitali Kamat , Nikil Kapur , Ross Kelly , Josh Owen , Ashi Rashid , William Stokes , Nicola William , Jeanine Williams , Andrew Nelson

Aminoquinolines (AQ) and substituted aminoquinolines (s-AQ) interact with electrochemically monitored supported dioleoyl phosphatidylcholine (DOPC) monolayers and immobilised artificial membranes (IAM) on HPLC column. The electrochemical sensor records adsorption/partition of the compound on and into the layer as well as specific interactions due to the location of the compound in the layer. HPLC-IAM technology measures the partition coefficient between the solution and phospholipid including partition due to interaction of the positive molecular charge with the phospholipid polar heads. The monolayer interaction results were combined and normalised for the neutral compounds’ lipophilicity as a log biomembrane affinity index (‘log BAI’) to exemplify charge and structural features in the interaction. A ChimeraX molecular modelling procedure was used to aid in the results interpretation. A compound ToxScore value was derived from 5 in vitro assays. The ‘log BAI’ exhibited a linear relationship with the AQ pK_a values showing that the interaction was related to the molecular positive charge and to the electron donating properties of the –NH₂ group. The correlation outliers showed a tendency/no tendency to H-bonding with the polar groups and a superficial/deeper location respectively in the phospholipid layer. The s-AQ ‘log BAI’ value displayed a power correlation with the compounds’ ToxScore values.

氨基喹啉（AQ）和取代氨基喹啉（s-AQ）与高效液相色谱柱上的电化学监测支撑二油酰磷脂酰胆碱（DOPC）单层和固定人工膜（IAM）相互作用。电化学传感器记录化合物在层上和层中的吸附/分离情况，以及化合物在层中的位置所导致的特定相互作用。HPLC-IAM 技术测量溶液与磷脂之间的分配系数，包括正分子电荷与磷脂极性头相互作用产生的分配。结合单层相互作用结果，并根据中性化合物的亲脂性将其归一化为生物膜亲和指数对数（"log BAI"），以体现相互作用中的电荷和结构特征。ChimeraX 分子建模程序用于帮助解释结果。从 5 项体外检测中得出了化合物 ToxScore 值。对数 BAI "与 AQ pKa 值呈线性关系，表明相互作用与分子正电荷和 -NH2 基团的电子捐赠特性有关。相关离群值分别显示了与极性基团的 H 键作用倾向/无倾向，以及在磷脂层中的浅层/深层位置。s-AQ 的 "对数 BAI "值与化合物的 ToxScore 值呈幂相关性。

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Corrigendum to “Analysis of electromagnetic response of cells and lipid membranes using a model-free method” [Bioelectrochemistry 152 (2023) 108444] 对 "利用无模型方法分析细胞和脂膜的电磁响应"[生物电化学 152 (2023) 108444] 的更正。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-02-01 DOI: 10.1016/j.bioelechem.2024.108841

Yingxian Lu , Xiaping Tang , Yanyu Zhao , Tianyu Jiang , Jiayao Zhou , Xiaofei Wang , Bing Huang , Lingyu Liu , Hu Deng , Yujing Huang , Yigong Shi

引用次数: 0

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