Sustainable graphene production remains challenging due to the high energy and environmental costs of conventional methods. This study demonstrates a renewable microalgae-based route for flash Joule heating synthesis of turbostratic graphene, elucidating the energy–structure–performance correlation toward low-carbon graphene manufacturing. Among the tested durations, a 500 ms flash yielded the best-performing sample (MDTG-500), which has a low defect density (Raman ID/IG = 0.38), a large interlayer spacing (0.344 nm), and a turbostratic few-layer structure confirmed by Raman, XRD, and TEM analyses. MDTG-500 also demonstrates excellent dispersibility, high electrical conductivity (63.8 S cm−1), strong broadband absorbance (∼89 %), and good thermal stability. When applied in supercapacitors, MDTG-500 exhibits ideal electric double-layer capacitance behavior, with a capacitance retention of 79.8 % from 1–100 mV s−1, a high areal capacitance of 84 µF cm−2, and outstanding cycling stability (106 % capacitance retention after 1000 CV cycles). This work provides a sustainable, efficient, and tunable strategy for producing high-purity turbostratic graphene from microalgae, offering new insights into biomass valorization and advanced carbon material design.
由于传统方法的高能源和环境成本，可持续的石墨烯生产仍然具有挑战性。本研究展示了一种基于可再生微藻的闪速焦耳加热合成涡轮石墨烯的途径，阐明了低碳石墨烯制造的能量-结构-性能相关性。在测试时间中，500 ms闪制得到了性能最好的样品（MDTG-500），该样品具有低缺陷密度（拉曼ID/IG = 0.38），层间间距大（0.344 nm），并且通过拉曼，XRD和TEM分析证实了其涡层结构。MDTG-500还具有优异的分散性、高导电性（63.8 S cm−1）、强宽带吸光度（~ 89%）和良好的热稳定性。当应用于超级电容器时，MDTG-500表现出理想的双电层电容行为，在1 - 100 mV s - 1范围内电容保持率为79.8%，面积电容高达84µF cm - 2，并且具有出色的循环稳定性（1000 CV循环后电容保持率为106%）。这项工作为从微藻中生产高纯度涡轮层石墨烯提供了一种可持续、高效和可调的策略，为生物质增值和先进的碳材料设计提供了新的见解。
{"title":"Sustainable production of Microalgae-Derived turbostratic graphene via flash Joule heating","authors":"Fuyuan Wang, Rui Huang, Yujie Yu, Shaojunyan Tan, Ruijie Yuan, Jianyong Yin, Shijie Zhang","doi":"10.1016/j.biortech.2025.133729","DOIUrl":"10.1016/j.biortech.2025.133729","url":null,"abstract":"<div><div>Sustainable graphene production remains challenging due to the high energy and environmental costs of conventional methods. This study demonstrates a renewable microalgae-based route for flash Joule heating synthesis of turbostratic graphene, elucidating the energy–structure–performance correlation toward low-carbon graphene manufacturing. Among the tested durations, a 500 ms flash yielded the best-performing sample (MDTG-500), which has a low defect density (Raman I<sub>D</sub>/I<sub>G</sub> = 0.38), a large interlayer spacing (0.344 nm), and a turbostratic few-layer structure confirmed by Raman, XRD, and TEM analyses. MDTG-500 also demonstrates excellent dispersibility, high electrical conductivity (63.8 S cm<sup>−1</sup>), strong broadband absorbance (∼89 %), and good thermal stability. When applied in supercapacitors, MDTG-500 exhibits ideal electric double-layer capacitance behavior, with a capacitance retention of 79.8 % from 1–100 mV s<sup>−1</sup>, a high areal capacitance of 84 µF cm<sup>−2</sup>, and outstanding cycling stability (106 % capacitance retention after 1000 CV cycles). This work provides a sustainable, efficient, and tunable strategy for producing high-purity turbostratic graphene from microalgae, offering new insights into biomass valorization and advanced carbon material design.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"442 ","pages":"Article 133729"},"PeriodicalIF":9.0,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145613853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-29DOI: 10.1016/j.biortech.2025.133749
Lu Mou , Runze Pan , Lianjie Wang , Lala Huang , Min Qiu , Wankui Jiang , Wenming Zhang , Fengxue Xin , Yujia Jiang , Min Jiang
The balanced hydrolase complexes are critical for efficient lignocellulose degradation, yet low β-glucosidase (BGL) activity is considered as the primary rate-limiting factor for cellulose hydrolysis by Trichoderma species. In this study, a dual-plate screening strategy with over 85 % positive mutation rate was designed, and a hypercellulolytic T. asperellum mutant ML02 was successfully isolated with approximately 2-fold increase of BGL activity after two rounds of ethyl methanesulfonate (EMS) mutagenesis. Phenotypic characterizations combined with genomic and transcriptomic analysis revealed that hyperbranching morphology, sporulation defects, and impaired cell wall integrity collectively contributed to the improved cellulase activity and cellulose degradation efficiency. Additionally, by constructing a microbial consortium composed of mutant ML02 and Lactobacillus paracasei, LA production from Avicel reached 72.65 g/L, with a cellulose degradation efficiency of 90 %. Besides, the LA yield from cellulose achieved 0.97 g/g, and over 87 % carbon flux from Avicel was channeled into LA synthesis. Importantly, the consortium also achieved an LA production of 60.74 g/L when used delignified rice straw as the sole carbon source, representing the highest reported performance for lignocellulosic LA production. These findings support that strain ML02 has strong potential to serve as an efficient microbial platform for cost-effective lignocellulose biorefining.
{"title":"Isolation of a Trichoderma asperellum mutant with high-level β-glucosidase activity and application for lignocellulose bioconversion","authors":"Lu Mou , Runze Pan , Lianjie Wang , Lala Huang , Min Qiu , Wankui Jiang , Wenming Zhang , Fengxue Xin , Yujia Jiang , Min Jiang","doi":"10.1016/j.biortech.2025.133749","DOIUrl":"10.1016/j.biortech.2025.133749","url":null,"abstract":"<div><div>The balanced hydrolase complexes are critical for efficient lignocellulose degradation, yet low β-glucosidase (BGL) activity is considered as the primary rate-limiting factor for cellulose hydrolysis by <em>Trichoderma</em> species. In this study, a dual-plate screening strategy with over 85 % positive mutation rate was designed, and a hypercellulolytic <em>T. asperellum</em> mutant ML02 was successfully isolated with approximately 2-fold increase of BGL activity after two rounds of ethyl methanesulfonate (EMS) mutagenesis. Phenotypic characterizations combined with genomic and transcriptomic analysis revealed that hyperbranching morphology, sporulation defects, and impaired cell wall integrity collectively contributed to the improved cellulase activity and cellulose degradation efficiency. Additionally, by constructing a microbial consortium composed of mutant ML02 and <em>Lactobacillus paracasei</em>, LA production from Avicel reached 72.65 g/L, with a cellulose degradation efficiency of 90 %. Besides, the LA yield from cellulose achieved 0.97 g/g, and over 87 % carbon flux from Avicel was channeled into LA synthesis. Importantly, the consortium also achieved an LA production of 60.74 g/L when used delignified rice straw as the sole carbon source, representing the highest reported performance for lignocellulosic LA production. These findings support that strain ML02 has strong potential to serve as an efficient microbial platform for cost-effective lignocellulose biorefining.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"442 ","pages":"Article 133749"},"PeriodicalIF":9.0,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-29DOI: 10.1016/j.biortech.2025.133742
Weiyuan Huang, Kunquan Li, Shengsheng Miao
The precise prediction of product distribution during crop biomass pyrolysis is critical for its valorization but remains challenging due to complex nonlinear interactions between feedstock heterogeneity and process parameters. This study developed an interpretable machine learning (ML) framework integrating Gradient Boosting Decision Trees (GBDT) optimized via Bayesian hyperparameter tuning (Optuna) to elucidate the coupling effects of proximate/ultimate composition and temperature on biochar, bio-oil, and gas yields. The GBDT model, trained on 265 data points from 28 types of crop residues, demonstrated the highest accuracy (test set R2 ≥ 0.89). Mechanistic interpretation using Shapley Additive exPlanations (SHAP) and Partial Dependence Plots (PDP) identified the O/C molar ratio as the most critical feature governing product distribution, with a threshold (O/C > 0.4) triggering pronounced deoxygenation towards gases. Ash content catalytically promoted secondary cracking reactions via inherent alkali metal oxides (e.g., K2O), reducing bio-oil yield, while pyrolysis temperature governed the selection of reaction pathways. Through the innovative integration of ML with Response Surface Methodology (RSM), distinct optimal process windows were established for maximizing each product: biochar (300 °C, 12 °C/min), bio-oil (523 °C, 79 °C/min), and gas (713 °C, 103 °C/min). Furthermore, an online predictive tool was developed to enable intelligent real-time regulation, effectively bridging the gap between model-based insight and industrial implementation of optimized pyrolysis processes.
{"title":"Real-time process design enabled by an interpretable GBDT model for high-fidelity prediction of pyrolysis products from crop residues","authors":"Weiyuan Huang, Kunquan Li, Shengsheng Miao","doi":"10.1016/j.biortech.2025.133742","DOIUrl":"10.1016/j.biortech.2025.133742","url":null,"abstract":"<div><div>The precise prediction of product distribution during crop biomass pyrolysis is critical for its valorization but remains challenging due to complex nonlinear interactions between feedstock heterogeneity and process parameters. This study developed an interpretable machine learning (ML) framework integrating Gradient Boosting Decision Trees (GBDT) optimized via Bayesian hyperparameter tuning (Optuna) to elucidate the coupling effects of proximate/ultimate composition and temperature on biochar, bio-oil, and gas yields. The GBDT model, trained on 265 data points from 28 types of crop residues, demonstrated the highest accuracy (test set R<sup>2</sup> ≥ 0.89). Mechanistic interpretation using Shapley Additive exPlanations (SHAP) and Partial Dependence Plots (PDP) identified the O/C molar ratio as the most critical feature governing product distribution, with a threshold (O/C > 0.4) triggering pronounced deoxygenation towards gases. Ash content catalytically promoted secondary cracking reactions via inherent alkali metal oxides (e.g., K<sub>2</sub>O), reducing bio-oil yield, while pyrolysis temperature governed the selection of reaction pathways. Through the innovative integration of ML with Response Surface Methodology (RSM), distinct optimal process windows were established for maximizing each product: biochar (300 °C, 12 °C/min), bio-oil (523 °C, 79 °C/min), and gas (713 °C, 103 °C/min). Furthermore, an online predictive tool was developed to enable intelligent real-time regulation, effectively bridging the gap between model-based insight and industrial implementation of optimized pyrolysis processes.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"442 ","pages":"Article 133742"},"PeriodicalIF":9.0,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-29DOI: 10.1016/j.biortech.2025.133748
Zirui Yin, Jiachen Ma, Ruoxuan Bian, Yiran Wang, Kaoming Zhang, Yanyan Ma, Xuxiang Zhang, Lin Ye
Activated sludge in wastewater treatment bioreactors plays a pivotal role in xenobiotic degradation but is also regarded as a hotspot for the dissemination of antibiotic resistance genes (ARGs). Yet, it remains unclear whether pollutant degradation itself also creates conditions that facilitate ARG spread. To address this, we developed a xenobiotic degradation gene (XDG) database covering 22 degradation pathways. Using this database, we analyzed over 30,000 complete bacterial genomes and identified widespread co-occurrence of ARGs and XDGs, particularly within Pseudomonadota and Campylobacterota. Metagenomic profiling of 119 activated sludge samples further revealed strong positive correlations (Pearson’s r > 0.8) between XDG and intrinsic ARGs, especially, modules involved in aromatic ring cleavage showed the highest correlations with ARGs. However, only 30.9 % of ARG–HGT events were found in MAGs carrying XDGs, and genome-level proximity analysis indicated that such microorganisms did not exhibit higher horizontal transfer potential. Cultivation-based experiments revealed that Pseudomonas strains with high degradation capacity carried intrinsic but not mobile ARGs. Together, these results demonstrate that xenobiotic degradation promotes ARG enrichment primarily through shifts in community composition rather than by enhancing gene mobility.
废水处理生物反应器中的活性污泥在外源生物降解中起着关键作用，但也被认为是抗生素耐药基因（ARGs）传播的热点。然而，尚不清楚污染物降解本身是否也创造了促进ARG传播的条件。为了解决这个问题，我们开发了一个涵盖22种降解途径的异种降解基因（XDG）数据库。利用该数据库，我们分析了超过30,000个完整的细菌基因组，并确定了ARGs和xdg广泛共存，特别是在假单胞菌和弯曲杆菌中。119个活性污泥样品的宏基因组分析进一步揭示了XDG与内在ARGs之间的强正相关（Pearson’s r > 0.8），特别是参与芳香环切割的模块与ARGs的相关性最高。然而，只有30.9%的ARG-HGT事件发生在携带xdg的MAGs中，基因组水平接近分析表明这些微生物没有表现出更高的水平转移潜力。基于培养的实验表明，具有高降解能力的假单胞菌菌株携带固有而非移动的ARGs。总之，这些结果表明，外源降解促进ARG富集主要是通过改变群落组成，而不是通过增强基因迁移。
{"title":"Xenobiotic degradation promotes enrichment but not dissemination of antibiotic resistance genes in activated sludge","authors":"Zirui Yin, Jiachen Ma, Ruoxuan Bian, Yiran Wang, Kaoming Zhang, Yanyan Ma, Xuxiang Zhang, Lin Ye","doi":"10.1016/j.biortech.2025.133748","DOIUrl":"10.1016/j.biortech.2025.133748","url":null,"abstract":"<div><div>Activated sludge in wastewater treatment bioreactors plays a pivotal role in xenobiotic degradation but is also regarded as a hotspot for the dissemination of antibiotic resistance genes (ARGs). Yet, it remains unclear whether pollutant degradation itself also creates conditions that facilitate ARG spread. To address this, we developed a xenobiotic degradation gene (XDG) database covering 22 degradation pathways. Using this database, we analyzed over 30,000 complete bacterial genomes and identified widespread co-occurrence of ARGs and XDGs, particularly within <em>Pseudomonadota</em> and <em>Campylobacterota</em>. Metagenomic profiling of 119 activated sludge samples further revealed strong positive correlations (Pearson’s r > 0.8) between XDG and intrinsic ARGs, especially, modules involved in aromatic ring cleavage showed the highest correlations with ARGs. However, only 30.9 % of ARG–HGT events were found in MAGs carrying XDGs, and genome-level proximity analysis indicated that such microorganisms did not exhibit higher horizontal transfer potential. Cultivation-based experiments revealed that <em>Pseudomonas</em> strains with high degradation capacity carried intrinsic but not mobile ARGs. Together, these results demonstrate that xenobiotic degradation promotes ARG enrichment primarily through shifts in community composition rather than by enhancing gene mobility.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"442 ","pages":"Article 133748"},"PeriodicalIF":9.0,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-29DOI: 10.1016/j.biortech.2025.133746
Haohao Mao , Ying Deng , Xuepeng Wang , Qilin Yu , Zonglu Yao , Zhiqiang Zhao , Ruixia Shen , Yaobin Zhang
Sub-thermophilic temperatures (41–49 °C) have been reported to improve anaerobic digestion, but the reason remains unclear. In this study, sub-thermophilic conditions were employed to promote the anaerobic digestion of cattle manure. The results showed that the methane production at 45 °C was 28.5 % higher than that at 37 °C. Under the sub-thermophilic temperatures, hydrolytic-acidogenic bacteria were enriched to increase the production of ethanol, acetate and butyrate and decrease the production of propionate, which provided available substrates for methanogenesis. It might compensate for the decrease in the key enzyme activity of methanogens at 45 °C. Also, the increase in ethanol helped the establishment of direct interspecies electron transfer and the activation of electron bifurcation mechanisms to maintain the survival of methanogens under the unfavorable temperature. Furthermore, the sub-thermophilic temperature also accelerated humification to improve digestate maturity. The study indicated that the two-phase anaerobic reactors might operate independently at their respective optimal temperatures.
{"title":"Sub-thermophilic anaerobic digestion: generation of favorable substrates for methanogenesis","authors":"Haohao Mao , Ying Deng , Xuepeng Wang , Qilin Yu , Zonglu Yao , Zhiqiang Zhao , Ruixia Shen , Yaobin Zhang","doi":"10.1016/j.biortech.2025.133746","DOIUrl":"10.1016/j.biortech.2025.133746","url":null,"abstract":"<div><div>Sub-thermophilic temperatures (41–49 °C) have been reported to improve anaerobic digestion, but the reason remains unclear. In this study, sub-thermophilic conditions were employed to promote the anaerobic digestion of cattle manure. The results showed that the methane production at 45 °C was 28.5 % higher than that at 37 °C. Under the sub-thermophilic temperatures, hydrolytic-acidogenic bacteria were enriched to increase the production of ethanol, acetate and butyrate and decrease the production of propionate, which provided available substrates for methanogenesis. It might compensate for the decrease in the key enzyme activity of methanogens at 45 °C. Also, the increase in ethanol helped the establishment of direct interspecies electron transfer and the activation of electron bifurcation mechanisms to maintain the survival of methanogens under the unfavorable temperature. Furthermore, the sub-thermophilic temperature also accelerated humification to improve digestate maturity. The study indicated that the two-phase anaerobic reactors might operate independently at their respective optimal temperatures.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"442 ","pages":"Article 133746"},"PeriodicalIF":9.0,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-29DOI: 10.1016/j.biortech.2025.133738
Lichong Duan , Shuyue Han , Zhiwen Zhang , Jiazhen Xu , Dan Wang , Shuang Chen , Jiqin Wang , Jinghua Lv , Chao Guo , Yunbei Li
The high water-holding capacity of extracellular polymeric substances (EPS) is a key factor contributing to sludge dewatering challenges. Intra- and inter-protein hydrogen bond networks within EPS play a critical role in water retention. In this study, a deep eutectic solvent (DES) was synthesized from choline chloride and ferric chloride hexahydrate to enhance activated sludge dewatering. Spectroscopic analysis and theoretical calculations were conducted to investigate changes in EPS composition and structure and the mechanisms underlying DES-induced modifications of the secondary structure and hydrophilicity of extracellular proteins. Under optimal conditions (200 rpm, 0.41 mL/g DES, 60 °C, 70 s), the capillary suction time of the conditioned sludge significantly decreased from 71.0 s to 29.7 s (approximately 58 % reduction). DES-coupled thermal treatment disrupted the sludge floc structure, neutralized the particle surface charge, and significantly enhanced surface hydrophobicity. Consequently, the contact angle of the treated sludge increased from approximately10° to over 35°. DES weakened the binding capacity of EPS, causing proteins and polysaccharides to transition from a bound state into the liquid phase. The treatment disrupted hydrogen bonds between protein backbones, reduced α-helix content, and increased β-sheet content, thereby altering the protein secondary structure. These structural changes exposed hydrophobic residues, which further enhanced sludge dewatering performance. Density-functional theory confirmed that DES disrupted protein backbone hydrogen bonds via non-covalent interactions, leading to structural rearrangement. This hydrogen bond-regulating strategy provides a green, efficient approach for sludge dewatering, with theoretical support for sustainable resource utilization.
{"title":"Hydrogen bond driven deep eutectic solvent strategy for enhanced sludge dewatering via protein network remodeling","authors":"Lichong Duan , Shuyue Han , Zhiwen Zhang , Jiazhen Xu , Dan Wang , Shuang Chen , Jiqin Wang , Jinghua Lv , Chao Guo , Yunbei Li","doi":"10.1016/j.biortech.2025.133738","DOIUrl":"10.1016/j.biortech.2025.133738","url":null,"abstract":"<div><div>The high water-holding capacity of extracellular polymeric substances (EPS) is a key factor contributing to sludge dewatering challenges. Intra- and inter-protein hydrogen bond networks within EPS play a critical role in water retention. In this study, a deep eutectic solvent (DES) was synthesized from choline chloride and ferric chloride hexahydrate to enhance activated sludge dewatering. Spectroscopic analysis and theoretical calculations were conducted to investigate changes in EPS composition and structure and the mechanisms underlying DES-induced modifications of the secondary structure and hydrophilicity of extracellular proteins. Under optimal conditions (200 rpm, 0.41 mL/g DES, 60 °C, 70 s), the capillary suction time of the conditioned sludge significantly decreased from 71.0 s to 29.7 s (approximately 58 % reduction). DES-coupled thermal treatment disrupted the sludge floc structure, neutralized the particle surface charge, and significantly enhanced surface hydrophobicity. Consequently, the contact angle of the treated sludge increased from approximately10° to over 35°. DES weakened the binding capacity of EPS, causing proteins and polysaccharides to transition from a bound state into the liquid phase. The treatment disrupted hydrogen bonds between protein backbones, reduced α-helix content, and increased β-sheet content, thereby altering the protein secondary structure. These structural changes exposed hydrophobic residues, which further enhanced sludge dewatering performance. Density-functional theory confirmed that DES disrupted protein backbone hydrogen bonds via non-covalent interactions, leading to structural rearrangement. This hydrogen bond-regulating strategy provides a green, efficient approach for sludge dewatering, with theoretical support for sustainable resource utilization.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"442 ","pages":"Article 133738"},"PeriodicalIF":9.0,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145613854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-29DOI: 10.1016/j.biortech.2025.133741
Bing Han , Zetao Huang , Zuhao Li , Zhige Zhang , Tao Tan , Jun Xie , Yong Chen
Methane reforming yields syngas with H2/CO ratios of 1 or 3, whereas the optimal ratio for methanol synthesis is 2. However, during long-term reforming, catalysts suffer deactivation from sintering and carbon deposition. Consequently, a general catalyst design principle tailored to industrial methanol synthesis is urgently needed. This study synthesized a high-entropy catalyst, NiLaMgAlCaCeOx, via mechanochemical ball milling, employing entropy engineering for Gibbs free energy reduction. The reforming performance of this high-entropy catalyst was evaluated using simulated biogas. An H2/CO ratio suitable for methanol production was achieved by adjusting H2O addition, and performance was compared with control catalysts. XPS, SEM, TEM, H2-TPD, TG, and FTIR techniques elucidated the high-entropy catalyst’s anti-sintering and carbon deposition resistance mechanisms. Results indicated that the high-entropy oxide structure in NiLaMgAlCaCeOx possesses high configurational entropy, which effectively restricts high-temperature active component migration and efficiently facilitates deposited carbon removal, preventing deactivation. This structure confers superior activity and stability compared to the control NiLaCeOx. Specifically, its reforming stability under simulated biogas surpassed that of control catalysts NiLaMgAlCeOx, NiLaMgCeOx, and NiLaCeOx. The key resistance against sintering and carbon deposition lies in high CO2 and H2O adsorption energy on the highentropy catalyst, coupled with multi-metal composition limiting Ni migration at elevated temperatures. This high-entropy stabilization mechanism, promoting resistance to sintering and mitigation of carbon deposition, offers valuable insights for future industrial methanol catalyst design.
{"title":"Design of highly efficient and carbon-resistant catalysts for biogas reforming using entropy engineering strategy","authors":"Bing Han , Zetao Huang , Zuhao Li , Zhige Zhang , Tao Tan , Jun Xie , Yong Chen","doi":"10.1016/j.biortech.2025.133741","DOIUrl":"10.1016/j.biortech.2025.133741","url":null,"abstract":"<div><div>Methane reforming yields syngas with H<sub>2</sub>/CO ratios of 1 or 3, whereas the optimal ratio for methanol synthesis is 2. However, during long-term reforming, catalysts suffer deactivation from sintering and carbon deposition. Consequently, a general catalyst design principle tailored to industrial methanol synthesis is urgently needed. This study synthesized a high-entropy catalyst, NiLaMgAlCaCeO<sub>x</sub>, via mechanochemical ball milling, employing entropy engineering for Gibbs free energy reduction. The reforming performance of this high-entropy catalyst was evaluated using simulated biogas. An H<sub>2</sub>/CO ratio suitable for methanol production was achieved by adjusting H<sub>2</sub>O addition, and performance was compared with control catalysts. XPS, SEM, TEM, H<sub>2</sub>-TPD, TG, and FTIR techniques elucidated the high-entropy catalyst’s anti-sintering and carbon deposition resistance mechanisms. Results indicated that the high-entropy oxide structure in NiLaMgAlCaCeO<sub>x</sub> possesses high configurational entropy, which effectively restricts high-temperature active component migration and efficiently facilitates deposited carbon removal, preventing deactivation. This structure confers superior activity and stability compared to the control NiLaCeO<sub>x</sub>. Specifically, its reforming stability under simulated biogas surpassed that of control catalysts NiLaMgAlCeO<sub>x</sub>, NiLaMgCeO<sub>x</sub>, and NiLaCeO<sub>x</sub>. The key resistance against sintering and carbon deposition lies in high CO<sub>2</sub> and H<sub>2</sub>O adsorption energy on the highentropy catalyst, coupled with multi-metal composition limiting Ni migration at elevated temperatures. This high-entropy stabilization mechanism, promoting resistance to sintering and mitigation of carbon deposition, offers valuable insights for future industrial methanol catalyst design.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"442 ","pages":"Article 133741"},"PeriodicalIF":9.0,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-29DOI: 10.1016/j.biortech.2025.133743
Fengjie Cui , Yuzhou Cao , Xuan Lou , Jun Liu , Aiqun Yu , Ning Xu
The non-conventional yeast Kluyveromyces marxianus is emerging as a versatile food-grade host with exceptional potential in industrial biotechnology, due to its rapid growth, thermotolerance, and metabolic flexibility. Its broad substrate utilization capacity and GRAS status have spurred growing interest in its application for recombinant protein expression and single-cell protein (SCP) production. However, a comprehensive understanding of the functional genomics and metabolic networks in K. marxianus remains limited. Nevertheless, ongoing efforts to develop diverse genetic engineering tools for K. marxianus have greatly strengthened its potential as a promising platform. In this review, we provide an extensive overview of the genetic, physiological, and biotechnological characteristics that establishes K. marxianus as an ideal host for efficient protein expression and SCP production. Recent advances and representative examples of engineering strategies aimed at unlocking its industrial potential for bioproduction were discussed. Finally, this review highlights the challenges and future directions for biotechnological innovations.
{"title":"Unlocking Kluyveromyces marxianus for efficient protein expression and single-cell protein production","authors":"Fengjie Cui , Yuzhou Cao , Xuan Lou , Jun Liu , Aiqun Yu , Ning Xu","doi":"10.1016/j.biortech.2025.133743","DOIUrl":"10.1016/j.biortech.2025.133743","url":null,"abstract":"<div><div>The non-conventional yeast <em>Kluyveromyces marxianus</em> is emerging as a versatile food-grade host with exceptional potential in industrial biotechnology, due to its rapid growth, thermotolerance, and metabolic flexibility. Its broad substrate utilization capacity and GRAS status have spurred growing interest in its application for recombinant protein expression and single-cell protein (SCP) production. However, a comprehensive understanding of the functional genomics and metabolic networks in <em>K. marxianus</em> remains limited. Nevertheless, ongoing efforts to develop diverse genetic engineering tools for <em>K. marxianus</em> have greatly strengthened its potential as a promising platform. In this review, we provide an extensive overview of the genetic, physiological, and biotechnological characteristics that establishes <em>K. marxianus</em> as an ideal host for efficient protein expression and SCP production. Recent advances and representative examples of engineering strategies aimed at unlocking its industrial potential for bioproduction were discussed. Finally, this review highlights the challenges and future directions for biotechnological innovations.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"442 ","pages":"Article 133743"},"PeriodicalIF":9.0,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-29DOI: 10.1016/j.biortech.2025.133734
Lucas Previtali Ferraz, Eric Keven Silva
Efficient recovery of oils and phenolics from fruit pomace using food-grade solvents is challenged by composition shifts and extraction kinetics. Here, a multi-stage ethanol–water biorefining of pomegranate pomace was benchmarked under stirred liquid (SLE), ultrasound-assisted (UAE), and pressurized liquid extraction (PLE) regimes. Early alcoholic fractions preserved punicic acid (C18:3n-5 ≈ 78 %), while later ones shifted toward higher saturation. PLE maximized phenolic yield (11.96 mg GAE g−1) and concentration per solvent unit, whereas UAE achieved faster concentration ratios (CR = 0.8 in 7.5 min). Hydroalcoholic extracts were dominated by punicalagin and ellagic acid, showing time-dependent hydrolysis. Representative extracts maintained antioxidant capacity during 28 days at 25 °C. Residual biomass exhibited higher protein retention after PLE and lignin-enriched signatures. Path2Green screening identified solvent recycling and energy efficiency as key sustainability levers. This kinetic mapping defines actionable operating windows for targeting oil quality, phenolic enrichment, and greener bioprocessing.
{"title":"Multi-stage ethanol–water biorefining of pomegranate pomace by-product enables time-resolved fractionation of fatty acids, phenolics and protein-rich residual biomass","authors":"Lucas Previtali Ferraz, Eric Keven Silva","doi":"10.1016/j.biortech.2025.133734","DOIUrl":"10.1016/j.biortech.2025.133734","url":null,"abstract":"<div><div>Efficient recovery of oils and phenolics from fruit pomace using food-grade solvents is challenged by composition shifts and extraction kinetics. Here, a multi-stage ethanol–water biorefining of pomegranate pomace was benchmarked under stirred liquid (SLE), ultrasound-assisted (UAE), and pressurized liquid extraction (PLE) regimes. Early alcoholic fractions preserved punicic acid (C18:3n-5 ≈ 78 %), while later ones shifted toward higher saturation. PLE maximized phenolic yield (11.96 mg GAE g<sup>−1</sup>) and concentration per solvent unit, whereas UAE achieved faster concentration ratios (CR = 0.8 in 7.5 min). Hydroalcoholic extracts were dominated by punicalagin and ellagic acid, showing time-dependent hydrolysis. Representative extracts maintained antioxidant capacity during 28 days at 25 °C. Residual biomass exhibited higher protein retention after PLE and lignin-enriched signatures. Path2Green screening identified solvent recycling and energy efficiency as key sustainability levers. This kinetic mapping defines actionable operating windows for targeting oil quality, phenolic enrichment, and greener bioprocessing.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"442 ","pages":"Article 133734"},"PeriodicalIF":9.0,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-29DOI: 10.1016/j.biortech.2025.133736
Hyeok Kim , Chungseob Lee , Myung Hee Kim , Go Eun Choi , Roent Dune A. Cayetano , Byung-Kook Hwang , Hyun-Suk Oh
Quorum quenching (QQ) technology, widely recognized for its effectiveness in mitigating membrane fouling in membrane bioreactors (MBRs), has been predominantly studied in laboratory-scale setups, with fewer applications in pilot-scale systems. Furthermore, most research in this field has been focused on using MBRs to treat synthetic wastewater or municipal sewage, with limited investigations into industrial wastewater treatment. In this study, we examined the feasibility of integrating QQ technology into a semi-pilot-scale MBR to treat wastewater from the display manufacturing industry and evaluated its antifouling performance. In semi-pilot-scale MBR operations, the QQ effect was associated with a significant delay in the increase in transmembrane pressure seen in an MBR without QQ. Simultaneously, we observed a reduction in N-acyl-homoserine lactone concentration in the sludge and extracellular polymeric substances in the biofilm attached to the membrane. The activity of the QQ beads remained stable and maintained their antifouling capability over 66 days of MBR operation. Microbial community analysis revealed that the QQ effect was correlated with changes in the abundance of specific microbial taxa (such as Rhizobiales) rather than causing significant shifts in the overall phylogenetic structure. The results of a functional gene analysis of acyl-homoserine lactone-related pathways indicate substantial reductions in related genes such as luxO, cciR, lasI, and bjaR1 in the QQ-MBR. These findings demonstrate that QQ technology can be effectively applied to full-scale MBR systems to treat industrial wastewater and offers a sustainable and efficient solution to mitigate biofouling challenges in wastewater treatment processes.
{"title":"Quorum quenching for biofouling control in a semi-pilot-scale membrane bioreactor treating display manufacturing wastewater","authors":"Hyeok Kim , Chungseob Lee , Myung Hee Kim , Go Eun Choi , Roent Dune A. Cayetano , Byung-Kook Hwang , Hyun-Suk Oh","doi":"10.1016/j.biortech.2025.133736","DOIUrl":"10.1016/j.biortech.2025.133736","url":null,"abstract":"<div><div>Quorum quenching (QQ) technology, widely recognized for its effectiveness in mitigating membrane fouling in membrane bioreactors (MBRs), has been predominantly studied in laboratory-scale setups, with fewer applications in pilot-scale systems. Furthermore, most research in this field has been focused on using MBRs to treat synthetic wastewater or municipal sewage, with limited investigations into industrial wastewater treatment. In this study, we examined the feasibility of integrating QQ technology into a semi-pilot-scale MBR to treat wastewater from the display manufacturing industry and evaluated its antifouling performance. In semi-pilot-scale MBR operations, the QQ effect was associated with a significant delay in the increase in transmembrane pressure seen in an MBR without QQ. Simultaneously, we observed a reduction in <em>N</em>-acyl-homoserine lactone concentration in the sludge and extracellular polymeric substances in the biofilm attached to the membrane. The activity of the QQ beads remained stable and maintained their antifouling capability over 66 days of MBR operation. Microbial community analysis revealed that the QQ effect was correlated with changes in the abundance of specific microbial taxa (such as Rhizobiales) rather than causing significant shifts in the overall phylogenetic structure. The results of a functional gene analysis of acyl-homoserine lactone-related pathways indicate substantial reductions in related genes such as <em>luxO</em>, <em>cciR</em>, <em>lasI</em>, and <em>bjaR1</em> in the QQ-MBR. These findings demonstrate that QQ technology can be effectively applied to full-scale MBR systems to treat industrial wastewater and offers a sustainable and efficient solution to mitigate biofouling challenges in wastewater treatment processes.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"442 ","pages":"Article 133736"},"PeriodicalIF":9.0,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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