Bioresource Technology最新文献

英文中文

Microbial-algal symbiotic system drives reconstruction of nitrogen, phosphorus, and methane cycles for purification of pollutants in aquaculture water 微生物-藻类共生系统推动氮、磷和甲烷循环的重建，以净化水产养殖用水中的污染物

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-14 DOI: 10.1016/j.biortech.2025.132531

Jun Gao , Qi Mang , Quanjie Li , Yi Sun , Gangchun Xu

Intensive aquaculture’s excessive nitrogen, phosphorus, and methane emissions caused environmental degradation. This study explored how algae-bacteria symbiotic systems (ABSS) enhanced water purification by regulating element cycles. We established a Chlorella pyrenoidosa-Bacillus subtilis symbiotic system. At a 1:1 bacteria-to-algae ratio, chlorophyll a and cell dry weight were highest. C. pyrenoidosa supplied organic acids, carbohydrates, and amino acids to B. subtilis, which reciprocated with amino acids, purines, and vitamins. ABSS significantly reduced total nitrogen, ammonia nitrogen (NH₄⁺-N), nitrite (NO₂⁻-N), nitrate (NO₃⁻-N), phosphate (PO₄³⁻-P), total phosphorous, dissolved organic carbon, and chemical oxygen demand in aquaculture water. It reshaped microbial communities and enriched key genus (Limnohabitans, Planktophila, Polaromonas, Methylocystis) and upregulating genes linked to organic phosphate mineralization, methane oxidation, and nitrate reduction. These changes strengthened nitrogen-phosphorus-methane cycle coupling, boosting water purification. ABSS offers an eco-engineering solution for aquaculture pollution by optimizing microbial interactions and nutrient cycling.

{"title":"Microbial-algal symbiotic system drives reconstruction of nitrogen, phosphorus, and methane cycles for purification of pollutants in aquaculture water","authors":"Jun Gao , Qi Mang , Quanjie Li , Yi Sun , Gangchun Xu","doi":"10.1016/j.biortech.2025.132531","DOIUrl":"10.1016/j.biortech.2025.132531","url":null,"abstract":"<div><div>Intensive aquaculture’s excessive nitrogen, phosphorus, and methane emissions caused environmental degradation. This study explored how algae-bacteria symbiotic systems (ABSS) enhanced water purification by regulating element cycles. We established a <em>Chlorella pyrenoidosa</em>-<em>Bacillus subtilis</em> symbiotic system. At a 1:1 bacteria-to-algae ratio, chlorophyll <em>a</em> and cell dry weight were highest. <em>C. pyrenoidosa</em> supplied organic acids, carbohydrates, and amino acids to <em>B. subtilis</em>, which reciprocated with amino acids, purines, and vitamins. ABSS significantly reduced total nitrogen, ammonia nitrogen (NH<sub>4</sub><sup>+</sup>-N), nitrite (NO<sub>2</sub><sup>−</sup>-N), nitrate (NO<sub>3</sub><sup>−</sup>-N), phosphate (PO<sub>4</sub><sup>3−</sup>-P), total phosphorous, dissolved organic carbon, and chemical oxygen demand in aquaculture water. It reshaped microbial communities and enriched key genus (<em>Limnohabitans</em>, <em>Planktophila</em>, <em>Polaromonas</em>, <em>Methylocystis</em>) and upregulating genes linked to organic phosphate mineralization, methane oxidation, and nitrate reduction. These changes strengthened nitrogen-phosphorus-methane cycle coupling, boosting water purification. ABSS offers an eco-engineering solution for aquaculture pollution by optimizing microbial interactions and nutrient cycling.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132531"},"PeriodicalIF":9.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large-sized aerobic granular biofilm: stable biotechnology to improve nitrogen removal and reduce sludge yield

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-14 DOI: 10.1016/j.biortech.2025.132543

Haibo Ma , Sihao Chen , Linhuan Lv , Zhou Ye , Jiaqi Yang , Binbin Wang , Jinte Zou , Jun Li , Ramon Ganigué

Three parallel sequencing batch reactors (control, small-sized polyurethane sponge (PUS) (3.0 mm), and large-sized PUS (10.0 mm)) were used to investigate aerobic granular biofilm (AGB) characteristics. Results show that 10.0 mm PUS facilitated rapid formation of large-sized AGB (AGB_L), which exhibited higher biomass concentration (8.5 g/L) and faster settling velocity (69.2–159.3 m/h) than aerobic granular sludge (AGS) (3.2 g/L and 38.6–80.0 m/h). The AGB_L system also maintained long-term structural stability with a lower instability coefficient (0.004–0.018 min⁻¹) than AGS (0.053–0.090 min⁻¹). Additionally, during long-term operation, the AGB_L system achieved excellent removal efficiencies for NH₄⁺-N (99.6 ± 0.4 %) and total nitrogen (92.3 ± 2.6 %), and exhibited a lower sludge yield (0.05 gVSS/gCOD) than AGS (0.14 gVSS/gCOD). The larger size and compact structure of AGB_L increased anoxic/anaerobic zones, enriching denitrifying and hydrolytic/fermentative bacteria. These findings highlight AGB_L with large PUS as a more promising biotechnology for practical applications than conventional AGS.

{"title":"Large-sized aerobic granular biofilm: stable biotechnology to improve nitrogen removal and reduce sludge yield","authors":"Haibo Ma , Sihao Chen , Linhuan Lv , Zhou Ye , Jiaqi Yang , Binbin Wang , Jinte Zou , Jun Li , Ramon Ganigué","doi":"10.1016/j.biortech.2025.132543","DOIUrl":"10.1016/j.biortech.2025.132543","url":null,"abstract":"<div><div>Three parallel sequencing batch reactors (control, small-sized polyurethane sponge (PUS) (3.0 mm), and large-sized PUS (10.0 mm)) were used to investigate aerobic granular biofilm (AGB) characteristics. Results show that 10.0 mm PUS facilitated rapid formation of large-sized AGB (AGB<sub>L</sub>), which exhibited higher biomass concentration (8.5 g/L) and faster settling velocity (69.2–159.3 m/h) than aerobic granular sludge (AGS) (3.2 g/L and 38.6–80.0 m/h). The AGB<sub>L</sub> system also maintained long-term structural stability with a lower instability coefficient (0.004–0.018 min<sup>−1</sup>) than AGS (0.053–0.090 min<sup>−1</sup>). Additionally, during long-term operation, the AGB<sub>L</sub> system achieved excellent removal efficiencies for NH<sub>4</sub><sup>+</sup>-N (99.6 ± 0.4 %) and total nitrogen (92.3 ± 2.6 %), and exhibited a lower sludge yield (0.05 gVSS/gCOD) than AGS (0.14 gVSS/gCOD). The larger size and compact structure of AGB<sub>L</sub> increased anoxic/anaerobic zones, enriching denitrifying and hydrolytic/fermentative bacteria. These findings highlight AGB<sub>L</sub> with large PUS as a more promising biotechnology for practical applications than conventional AGS.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132543"},"PeriodicalIF":9.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deuterium stress Reprograms Chlorella sorokiniana Metabolism: Coupling photosynthetic suppression with carbon Reserve Surge

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-14 DOI: 10.1016/j.biortech.2025.132548

Tianfei Li, Yixian He, Shanni Ke, Zexia Cai, Zhuo Jiang

Microalgae convert inorganic substrates into stable isotope-labeled compounds, ideal for deuterated compound production. However, the mechanism by which deuterium affects the growth and metabolism of microalgae remains unclear. This study aims to reveal the effects of deuterium on the growth and metabolic processes of Chlorella sorokiniana and to clarify the interaction between them. After deuterium treatment, cell growth and the accumulation of photosynthetic pigments were significantly inhibited, leading to reduced photosynthetic efficiency and blocked energy transfer. Under 100% D₂O conditions, the accumulation of starch and lipids was enhanced, with starch content reaching up to 52% of dry cell weight and lipid content reaching 22%. Transcriptomics revealed deuterium stress inhibited photosynthesis-related genes while upregulating pathways for starch, fatty acid, and nucleic acid synthesis. These findings reveal the adaptation mechanism of microalgae to deuterium treatment and provide valuable insights for the utilization of microalgae in the production of deuterated organic compounds.

{"title":"Deuterium stress Reprograms Chlorella sorokiniana Metabolism: Coupling photosynthetic suppression with carbon Reserve Surge","authors":"Tianfei Li, Yixian He, Shanni Ke, Zexia Cai, Zhuo Jiang","doi":"10.1016/j.biortech.2025.132548","DOIUrl":"10.1016/j.biortech.2025.132548","url":null,"abstract":"<div><div>Microalgae convert inorganic substrates into stable isotope-labeled compounds, ideal for deuterated compound production. However, the mechanism by which deuterium affects the growth and metabolism of microalgae remains unclear. This study aims to reveal the effects of deuterium on the growth and metabolic processes of <em>Chlorella sorokiniana</em> and to clarify the interaction between them. After deuterium treatment, cell growth and the accumulation of photosynthetic pigments were significantly inhibited, leading to reduced photosynthetic efficiency and blocked energy transfer. Under 100% D<sub>2</sub>O conditions, the accumulation of starch and lipids was enhanced, with starch content reaching up to 52% of dry cell weight and lipid content reaching 22%. Transcriptomics revealed deuterium stress inhibited photosynthesis-related genes while upregulating pathways for starch, fatty acid, and nucleic acid synthesis. These findings reveal the adaptation mechanism of microalgae to deuterium treatment and provide valuable insights for the utilization of microalgae in the production of deuterated organic compounds.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132548"},"PeriodicalIF":9.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-situ cascaded and selective bio-preparation of galactose-derived acids from cheese whey powder by the bifunctional regulation of resting-cell catalysis

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-14 DOI: 10.1016/j.biortech.2025.132527

Xia Hua , Rui Zhang , Yating Hu , Dylan Liu , Yong Xu

To valorize the excess galactose resources and prepare value-added chemicals for applications in the food and biomedical industries, Gluconobacter oxydans was employed to realize the cascaded and selective bio-preparation of galactose-derived acids. The bifunctional regulatory mechanism of Ca²⁺ was the in-situ controllable preparation of calcium galactoate and 2-ketogalactonate by cleverly employing solubility difference. The presence of Ca²⁺ could additionally intensify bioconversion efficiency, leading to not only increased bioconversion rate to 1.5 g/L/h, but also improved yield by 18 %. Finally, by combining enzyme hydrolysis, yeast and bacterial fermentation, 100 g cheese whey powder was effectively bio-transformed into 22.2 g bioethanol, 16.8 galactonic acid and 34.4 g 2-ketogalactonic acid, with glucose and galactose utilization rate of 83.4 % and 97.1 %. Overall, this cascading and precise regulation process not only provided a reliable idea for the downstream outlet of galactose, importantly, but also established a potential production technology for 2-ketogalactonic acid.

{"title":"In-situ cascaded and selective bio-preparation of galactose-derived acids from cheese whey powder by the bifunctional regulation of resting-cell catalysis","authors":"Xia Hua , Rui Zhang , Yating Hu , Dylan Liu , Yong Xu","doi":"10.1016/j.biortech.2025.132527","DOIUrl":"10.1016/j.biortech.2025.132527","url":null,"abstract":"<div><div>To valorize the excess galactose resources and prepare value-added chemicals for applications in the food and biomedical industries, <em>Gluconobacter oxydans</em> was employed to realize the cascaded and selective bio-preparation of galactose-derived acids. The bifunctional regulatory mechanism of Ca<sup>2+</sup> was the <em>in-situ</em> controllable preparation of calcium galactoate and 2-ketogalactonate by cleverly employing solubility difference. The presence of Ca<sup>2+</sup> could additionally intensify bioconversion efficiency, leading to not only increased bioconversion rate to 1.5 g/L/h, but also improved yield by 18 %. Finally, by combining enzyme hydrolysis, yeast and bacterial fermentation, 100 g cheese whey powder was effectively bio-transformed into 22.2 g bioethanol, 16.8 galactonic acid and 34.4 g 2-ketogalactonic acid, with glucose and galactose utilization rate of 83.4 % and 97.1 %. Overall, this cascading and precise regulation process not only provided a reliable idea for the downstream outlet of galactose, importantly, but also established a potential production technology for 2-ketogalactonic acid.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132527"},"PeriodicalIF":9.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards robust partial nitrification in low-ammonia wastewater: Electrospinning nanofiber composite-enhanced hydrogel beads immobilized comammox Nitrospira

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-13 DOI: 10.1016/j.biortech.2025.132541

Peng Tang , Liangang Hou , Meiling Yin , Feng Huang , Zhengwei Pan , Tianhao Shi , Jun Li , Yuhan Zhu , Xin Zhang , Peng Gao

Stable partial nitrification (PN) in low-ammonia wastewater has been a critical challenge, but comammox Nitrospira shows great potential in PN systems due to unique physiological characteristics. Polyvinyl alcohol/sodium alginate electrospinning nanofiber (PVA/SA-EN) and polyvinylidene fluoride EN (PVDF-EN) were used to construct EN hydrogel beads (ENHB) with sandwich and core–shell structures, respectively. Comammox HB (cHB) and three types of comammox ENHB (cENHB) were assembled by immobilizing comammox sludge and operated for 60 days in low-ammonia wastewater. Results showed a significant correlation between PN performance, HB pore structure, and comammox Nitrospira abundance. With superior pore structure and mechanical strength, PVA/SA-PVDF-cENHB achieved a nitrite accumulation rate of 55.08 %, indicating enhanced PN performance. The contribution of comammox Nitrospira to PN was 73.19 %. Its abundance in PVA/SA-PVDF-cENHB was 5.56 × 10⁶ copies/(g sludge), 1.16–1.95-fold higher than the other three HB. Nanofiber composite-enhanced hydrogel immobilizes comammox Nitrospira provides new ideas for achieving robust PN in low-ammonia wastewater.

{"title":"Towards robust partial nitrification in low-ammonia wastewater: Electrospinning nanofiber composite-enhanced hydrogel beads immobilized comammox Nitrospira","authors":"Peng Tang , Liangang Hou , Meiling Yin , Feng Huang , Zhengwei Pan , Tianhao Shi , Jun Li , Yuhan Zhu , Xin Zhang , Peng Gao","doi":"10.1016/j.biortech.2025.132541","DOIUrl":"10.1016/j.biortech.2025.132541","url":null,"abstract":"<div><div>Stable partial nitrification (PN) in low-ammonia wastewater has been a critical challenge, but comammox <em>Nitrospira</em> shows great potential in PN systems due to unique physiological characteristics. Polyvinyl alcohol/sodium alginate electrospinning nanofiber (PVA/SA-EN) and polyvinylidene fluoride EN (PVDF-EN) were used to construct EN hydrogel beads (ENHB) with sandwich and core–shell structures, respectively. Comammox HB (cHB) and three types of comammox ENHB (cENHB) were assembled by immobilizing comammox sludge and operated for 60 days in low-ammonia wastewater. Results showed a significant correlation between PN performance, HB pore structure, and comammox <em>Nitrospira</em> abundance. With superior pore structure and mechanical strength, PVA/SA-PVDF-cENHB achieved a nitrite accumulation rate of 55.08 %, indicating enhanced PN performance. The contribution of comammox <em>Nitrospira</em> to PN was 73.19 %. Its abundance in PVA/SA-PVDF-cENHB was 5.56 × 10⁶ copies/(g sludge), 1.16–1.95-fold higher than the other three HB. Nanofiber composite-enhanced hydrogel immobilizes comammox <em>Nitrospira</em> provides new ideas for achieving robust PN in low-ammonia wastewater.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132541"},"PeriodicalIF":9.7,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Degradation of organic pollutant by natural biofilm based biophotovoltaic cells: The combined role of illumination, reactive oxygen species, and enhanced electron transfer

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-13 DOI: 10.1016/j.biortech.2025.132537

Zihan Qi, Xiuyi Hua, Anfeng Li, Haiyang Liu, Deming Dong, Dapeng Liang, Zhiyong Guo, Na Zheng

Autotrophic biofilms in natural water can produce reactive oxygen species (ROS) and degrade organic pollutants. To test the feasibility of applying natural biofilms in biophotovoltaic (BPV) cells, a simple single-chamber BPV using biofilms developed in lake water as the microbial anode was constructed, and electricity production and pollutant removal capacity of the BPV were evaluated. The natural biofilm BPV (NB-BPV) established was a robust and self-sustaining BPV with positive light response in electricity generation and sustainable power generation in the dark. Under visible light illumination, the voltage of the NB-BPV reached a maximum output of 150.6 ± 0.7 mV, with a power density of 326.4 μW/m² (per electrode surface area). Meanwhile, it could effectively degrade sodium dodecylbenzene sulfonate (DBS), while generating electricity, and the removal rate of DBS and TOC in 36 h was 77.1 % and 53.2 %, respectively. Under sunlight, NB-BPV could also produce electricity steadily in lake and the removal rate of DBS in simulated lake water was 93.7 % (120 h). Visible light significantly affected the performance of NB-BPV mainly through photosynthesis. Photosynthesis of biofilm promoted electricity generation and significantly enhanced the degradation of DBS by promoting electron transfer activity and generating ROS. Compared with biofilm system, the closed-circuit in NB-BPV promoted electron transfer, allowing more efficient degradation of DBS at relatively low level of ROS. Such a novel self-sustainable BPV has the potential to degrade refractory pollutants, and to be used in natural water.

{"title":"Degradation of organic pollutant by natural biofilm based biophotovoltaic cells: The combined role of illumination, reactive oxygen species, and enhanced electron transfer","authors":"Zihan Qi, Xiuyi Hua, Anfeng Li, Haiyang Liu, Deming Dong, Dapeng Liang, Zhiyong Guo, Na Zheng","doi":"10.1016/j.biortech.2025.132537","DOIUrl":"10.1016/j.biortech.2025.132537","url":null,"abstract":"<div><div>Autotrophic biofilms in natural water can produce reactive oxygen species (ROS) and degrade organic pollutants. To test the feasibility of applying natural biofilms in biophotovoltaic (BPV) cells, a simple single-chamber BPV using biofilms developed in lake water as the microbial anode was constructed, and electricity production and pollutant removal capacity of the BPV were evaluated. The natural biofilm BPV (NB-BPV) established was a robust and self-sustaining BPV with positive light response in electricity generation and sustainable power generation in the dark. Under visible light illumination, the voltage of the NB-BPV reached a maximum output of 150.6 ± 0.7 mV, with a power density of 326.4 μW/m<sup>2</sup> (per electrode surface area). Meanwhile, it could effectively degrade sodium dodecylbenzene sulfonate (DBS), while generating electricity, and the removal rate of DBS and TOC in 36 h was 77.1 % and 53.2 %, respectively. Under sunlight, NB-BPV could also produce electricity steadily in lake and the removal rate of DBS in simulated lake water was 93.7 % (120 h). Visible light significantly affected the performance of NB-BPV mainly through photosynthesis. Photosynthesis of biofilm promoted electricity generation and significantly enhanced the degradation of DBS by promoting electron transfer activity and generating ROS. Compared with biofilm system, the closed-circuit in NB-BPV promoted electron transfer, allowing more efficient degradation of DBS at relatively low level of ROS. Such a novel self-sustainable BPV has the potential to degrade refractory pollutants, and to be used in natural water.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132537"},"PeriodicalIF":9.7,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in multi-omics technologies for identifying metabolic engineering targets and improving lipid production in microalgae 多组学技术在确定代谢工程目标和提高微藻类脂质产量方面的进展

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-12 DOI: 10.1016/j.biortech.2025.132501

Chun-xiao Yan , Shuai Zhang , Lu-wei Xu , Han Gao , Zi-xu Zhang , Wang Ma , Xiao-man Sun

Polyunsaturated fatty acids (PUFAs), such as γ-linolenic acid, arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid, are highly valued in the global market due to their physiological effects and health benefits. Concerns related to overfishing and marine ecosystem degradation have driven interest in microalgal lipids as a sustainable and eco-friendly alternative for PUFA production. Despite some success in commercializing microalgal lipid products, they still fail to meet global demand. Advances in high-throughput omics technologies, including genomics, transcriptomics, proteomics, and metabolomics, have deepened the understanding of lipid biosynthesis in microalgae. This review explores the potential of multi-omics approaches to elucidate PUFA biosynthesis pathways, identify key regulatory genes, and optimize metabolic engineering strategies for enhanced lipid production. Additionally, this review discusses how multi-omics technologies address challenges in large-scale cultivation, promoting the industrialization of microalgal lipid productions. These insights provide a foundation for improving microalgal PUFA yields to meet growing global demand.

多不饱和脂肪酸（PUFAs），如γ-亚麻酸、花生四烯酸、二十碳五烯酸和二十二碳六烯酸，由于其生理作用和健康益处，在全球市场上具有很高的价值。人们对过度捕捞和海洋生态系统退化的担忧推动了人们对微藻脂的兴趣，将其作为生产 PUFA 的可持续和生态友好型替代品。尽管微藻脂质产品的商业化取得了一些成功，但仍无法满足全球需求。包括基因组学、转录组学、蛋白质组学和代谢组学在内的高通量组学技术的进步加深了人们对微藻脂质生物合成的了解。本综述探讨了多组学方法在阐明 PUFA 生物合成途径、确定关键调控基因和优化代谢工程策略以提高脂质产量方面的潜力。此外，本综述还讨论了多组学技术如何解决大规模栽培中的挑战，促进微藻脂质生产的工业化。这些见解为提高微藻 PUFA 产量以满足日益增长的全球需求奠定了基础。

{"title":"Advances in multi-omics technologies for identifying metabolic engineering targets and improving lipid production in microalgae","authors":"Chun-xiao Yan , Shuai Zhang , Lu-wei Xu , Han Gao , Zi-xu Zhang , Wang Ma , Xiao-man Sun","doi":"10.1016/j.biortech.2025.132501","DOIUrl":"10.1016/j.biortech.2025.132501","url":null,"abstract":"<div><div>Polyunsaturated fatty acids (PUFAs), such as γ-linolenic acid, arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid, are highly valued in the global market due to their physiological effects and health benefits. Concerns related to overfishing and marine ecosystem degradation have driven interest in microalgal lipids as a sustainable and eco-friendly alternative for PUFA production. Despite some success in commercializing microalgal lipid products, they still fail to meet global demand. Advances in high-throughput omics technologies, including genomics, transcriptomics, proteomics, and metabolomics, have deepened the understanding of lipid biosynthesis in microalgae. This review explores the potential of multi-omics approaches to elucidate PUFA biosynthesis pathways, identify key regulatory genes, and optimize metabolic engineering strategies for enhanced lipid production. Additionally, this review discusses how multi-omics technologies address challenges in large-scale cultivation, promoting the industrialization of microalgal lipid productions. These insights provide a foundation for improving microalgal PUFA yields to meet growing global demand.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132501"},"PeriodicalIF":9.7,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrostatically assembled maghemite nanoparticles-Lactobacillus plantarum: A novel hybrid for enhanced antioxidant, antimicrobial, and antibiofilm efficacy

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-12 DOI: 10.1016/j.biortech.2025.132538

Jayshri A. Shingade , Navnath S. Padalkar , Jae Hwan Shin , Yeong Hyeock Kim , Tae Jung Park , Jong Pil Park , Abhinandan R. Patil

Excessive antibiotic use contributes to oxidative stress and microbial imbalance, leading to increased growth of pathogens and biofilm formation. To address this, we developed a novel electrostatically assembled hybrid of maghemite nanoparticles and Lactobacillus plantarum (MNPs-LAB) as a multifunctional agent. Structural and surface interactions were confirmed through X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS), while transmission electron microscopy (TEM) and field emission scanning electron microscopy (FESEM) analyses revealed a uniform distribution of MNPs on the LAB surface. The MNPs-LAB hybrid exhibited strong antioxidant activity (71.45 % at 500 µg/mL) and enhanced antimicrobial performance against Listeria monocytogenes. In addition, the hybrid inhibited biofilm formation and effectively eradicated preformed biofilms of Staphylococcus succinus, Listeria monocytogenes, Escherichia coli, and Salmonella thompson. Notably, the low cytotoxicity observed in Caco-2 cells indicated good biocompatibility with intestinal epithelial cells. These results highlight the potential of MNPs-LAB hybrid as a safe and effective therapeutic candidate for combating oxidative stress, microbial infections, and biofilm-associated challenges.

{"title":"Electrostatically assembled maghemite nanoparticles-Lactobacillus plantarum: A novel hybrid for enhanced antioxidant, antimicrobial, and antibiofilm efficacy","authors":"Jayshri A. Shingade , Navnath S. Padalkar , Jae Hwan Shin , Yeong Hyeock Kim , Tae Jung Park , Jong Pil Park , Abhinandan R. Patil","doi":"10.1016/j.biortech.2025.132538","DOIUrl":"10.1016/j.biortech.2025.132538","url":null,"abstract":"<div><div>Excessive antibiotic use contributes to oxidative stress and microbial imbalance, leading to increased growth of pathogens and biofilm formation. To address this, we developed a novel electrostatically assembled hybrid of maghemite nanoparticles and <em>Lactobacillus plantarum</em> (MNPs-<em>LAB</em>) as a multifunctional agent. Structural and surface interactions were confirmed through X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS), while transmission electron microscopy (TEM) and field emission scanning electron microscopy (FESEM) analyses revealed a uniform distribution of MNPs on the <em>LAB</em> surface. The MNPs-<em>LAB</em> hybrid exhibited strong antioxidant activity (71.45 % at 500 µg/mL) and enhanced antimicrobial performance against <em>Listeria monocytogenes</em>. In addition, the hybrid inhibited biofilm formation and effectively eradicated preformed biofilms of <em>Staphylococcus succinus</em>, <em>Listeria monocytogenes</em>, <em>Escherichia coli</em>, and <em>Salmonella thompson.</em> Notably, the low cytotoxicity observed in Caco-2 cells indicated good biocompatibility with intestinal epithelial cells. These results highlight the potential of MNPs-<em>LAB</em> hybrid as a safe and effective therapeutic candidate for combating oxidative stress, microbial infections, and biofilm-associated challenges.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132538"},"PeriodicalIF":9.7,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial inoculant accelerated pig slurry maturation at low-temperature anaerobic digestion: Performance and mechanism

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-12 DOI: 10.1016/j.biortech.2025.132532

Yali Yang , Zhe Zhao , Dengchao Lei , Hongbo He , Hongtu Xie , Xudong Zhang , Shijun Sun , Xuelian Bao , Yulan Zhang

This study investigated the impact of microbial inoculants on maturing pig slurry during anaerobic digestion (AD) at psychrophilic temperatures (10 °C). A simulation experiment was conducted with two treatments: pig slurry alone (CK) and pig slurry supplemented with microbial inoculants (MA). The MA treatment achieved faster maturity than CK, as evidenced by an improved germination index (achieving 70 % in advance) and a reduced content of organic pollutants, which was attributed to an increased relative abundance of hydrolytic microorganisms (7–21 %) and decreased pathogens (6–9 %) at the early stage (<42 (d)). Microbial inoculants influenced pig slurry microorganisms by serving as keystone taxa (B_OTU229 (f_Ruminococcaceae) and F_OTU76 (s_Acremonium alcalophilum)), and stimulating new keystone taxa (B_OTU423 (g_NK4A214) and F_OTU25 (g_Clavulina)) through associative interactions, thereby intensifying saprotrophic and carbon cycling processes, and accelerating maturation. These results provide a biological strategy for maturing pig slurry and help elucidate the mechanisms underlying microbial effects.

{"title":"Microbial inoculant accelerated pig slurry maturation at low-temperature anaerobic digestion: Performance and mechanism","authors":"Yali Yang , Zhe Zhao , Dengchao Lei , Hongbo He , Hongtu Xie , Xudong Zhang , Shijun Sun , Xuelian Bao , Yulan Zhang","doi":"10.1016/j.biortech.2025.132532","DOIUrl":"10.1016/j.biortech.2025.132532","url":null,"abstract":"<div><div>This study investigated the impact of microbial inoculants on maturing pig slurry during anaerobic digestion (AD) at psychrophilic temperatures (10 °C). A simulation experiment was conducted with two treatments: pig slurry alone (CK) and pig slurry supplemented with microbial inoculants (MA). The MA treatment achieved faster maturity than CK, as evidenced by an improved germination index (achieving 70 % in advance) and a reduced content of organic pollutants, which was attributed to an increased relative abundance of hydrolytic microorganisms (7–21 %) and decreased pathogens (6–9 %) at the early stage (<42 (d)). Microbial inoculants influenced pig slurry microorganisms by serving as keystone taxa (<em>B_OTU229</em> (<em>f_Ruminococcaceae</em>) and <em>F_OTU76</em> (<em>s_Acremonium alcalophilum</em>)), and stimulating new keystone taxa (<em>B_OTU423</em> (<em>g_NK4A214</em>) and <em>F_OTU25</em> (<em>g_Clavulina</em>)) through associative interactions, thereby intensifying saprotrophic and carbon cycling processes, and accelerating maturation. These results provide a biological strategy for maturing pig slurry and help elucidate the mechanisms underlying microbial effects.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132532"},"PeriodicalIF":9.7,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of potential prebiotic oligosaccharides with diverse glycosidic linkages from corn stover via simultaneous hydrolysis and glycosylation

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-12 DOI: 10.1016/j.biortech.2025.132486

Meijun Zeng , Jee-Hwan Oh , Jan-Peter van Pijkeren , Xuejun Pan

This study presents the synthesis of potential prebiotic oligosaccharides directly from corn stover using concentrated sulfuric acid as both solvent and catalyst under mild conditions. The process involves dissolving corn stover at 20 °C for 1 h, followed by simultaneous hydrolysis of cellulose and hemicellulose and glycosylation of the resulting mono- and oligo-saccharides at 50 °C for 10 min in 80 wt% H₂SO₄. A yield of 48.8 % of corn stover-derived oligosaccharides (CS-OS) was achieved, nearing the theoretical yield. Structural analysis revealed that CS-OS were primarily gluco-oligosaccharides (average degree of polymerization (DP) 4.3) and xylo-oligosaccharides (average DP 3.6) with diverse α/β-(1→6/4/3/2/1) glycosidic bonds. Preliminary in vitro evaluation demonstrated that CS-OS effectively supported the growth of three probiotic strains (Lactobacillus and Pediococcus), outperforming commercial prebiotic isomalto-oligosaccharides and matching commercial prebiotic xylo-oligosaccharides. This study offers a valuable method to convert agricultural waste lignocellulosic biomass into valuable prebiotic oligosaccharides.

{"title":"Synthesis of potential prebiotic oligosaccharides with diverse glycosidic linkages from corn stover via simultaneous hydrolysis and glycosylation","authors":"Meijun Zeng , Jee-Hwan Oh , Jan-Peter van Pijkeren , Xuejun Pan","doi":"10.1016/j.biortech.2025.132486","DOIUrl":"10.1016/j.biortech.2025.132486","url":null,"abstract":"<div><div>This study presents the synthesis of potential prebiotic oligosaccharides directly from corn stover using concentrated sulfuric acid as both solvent and catalyst under mild conditions. The process involves dissolving corn stover at 20 °C for 1 h, followed by simultaneous hydrolysis of cellulose and hemicellulose and glycosylation of the resulting mono- and oligo-saccharides at 50 °C for 10 min in 80 wt% H<sub>2</sub>SO<sub>4</sub>. A yield of 48.8 % of corn stover-derived oligosaccharides (CS-OS) was achieved, nearing the theoretical yield. Structural analysis revealed that CS-OS were primarily gluco-oligosaccharides (average degree of polymerization (DP) 4.3) and xylo-oligosaccharides (average DP 3.6) with diverse α/β-(1→6/4/3/2/1) glycosidic bonds. Preliminary <em>in vitro</em> evaluation demonstrated that CS-OS effectively supported the growth of three probiotic strains (<em>Lactobacillus</em> and <em>Pediococcus</em>), outperforming commercial prebiotic isomalto-oligosaccharides and matching commercial prebiotic xylo-oligosaccharides. This study offers a valuable method to convert agricultural waste lignocellulosic biomass into valuable prebiotic oligosaccharides.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132486"},"PeriodicalIF":9.7,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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