Bioresource Technology最新文献_第4页

Perfluorooctane sulfonate (PFOS) inhibits methane production during sludge anaerobic digestion by breaking the carbon-transfer bridge between methanogenesis and acidogenesis 全氟辛烷磺酸（PFOS）通过破坏甲烷生成和酸生成之间的碳传递桥梁，抑制污泥厌氧消化过程中的甲烷生成

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-04 DOI: 10.1016/j.biortech.2025.133775

Zhaobin Liu, Yijing Gao, Jiayi Wang, Xinyu Jing, Xue Chen, Weijie Hu, Xueqin Lu, Meng Liu, Xiaosong He, Gopalakrishnan Kumar, Guangyin Zhen

Per- or polyfluoroalkyl compounds (PFASs) are recognized as emerging contaminant, with perfluorooctane sulfonate (PFOS) being one of the most extensively utilized PFASs due to its great chemical stability. However, knowledge of the bio-chemical behavior, the toxicity of PFOS and its mechanisms of interfacial binding to microorganisms remain inadequately validated. In this study, the biotoxicity of PFOS and its molecular interfacial adsorption mechanism in anaerobic digestion were investigated. The results showed that the tightly bound EPS (TB-EPS) of anaerobic microorganisms could defend against the biotoxicity of PFOS to some extent by physical adsorption and chemical binding, the exposure to PFOS might produce a greater disturbance to methanogenic archaea. With the increase of PFOS, acid-producing bacteria (APB) and methanogenic archaea showed different resistance to PFOS, suppressing cumulative methane production by up to 91.64 %. On the contrary, APBs were more tolerant, and fatty acids accumulated up to 2194.27 mg/L. Metagenomics analysis further confirmed that functional genes associated with fatty acid biosynthesis (fas, FAS2, fabK, etc.) were significantly enriched (approximately 85.29 %) whereas the relative abundance of genes associated with methanogenesis (acs, comA, mcrB, etc.) were decreased (up to 65.96 %). Molecular docking results suggested a potential route for PFOS cellular entry, as it was observed to bind to the substrate-binding protein of the ATP-binding cassette (ABC) transporter and interact with key functional enzymes, which led to the inhibition of methanogens. This study provides novel insights into the molecular blocking mechanism by which PFOS disrupts carbon metabolic flux through the selective inhibition of methanogenic archaea, rather than through a general suppression of acidogenic bacteria.

全氟烷基或多氟烷基化合物（PFASs）是公认的新兴污染物，全氟辛烷磺酸（PFOS）因其具有很强的化学稳定性而成为使用最广泛的全氟烷基化合物之一。然而，对全氟辛烷磺酸的生化行为、毒性及其与微生物的界面结合机制的了解仍然不够充分。研究了全氟辛烷磺酸的生物毒性及其在厌氧消化中的分子界面吸附机理。结果表明，厌氧微生物紧密结合的EPS （TB-EPS）能够通过物理吸附和化学结合在一定程度上抵御全氟辛烷磺酸的生物毒性，暴露于全氟辛烷磺酸可能对产甲烷古菌产生较大的干扰。随着PFOS浓度的升高，产酸菌（APB）和产甲烷古菌对PFOS表现出不同的抗性，对累积产甲烷的抑制幅度高达91.64 %。相反，APBs的耐受性更强，脂肪酸累积量高达2194.27 mg/L。宏基因组学分析进一步证实，与脂肪酸生物合成相关的功能基因（fas, FAS2， fabK等）显著富集（约85.29 %），而与甲烷生成相关的基因（acs, comA， mcrB等）的相对丰度降低（高达65.96 %）。分子对接结果提示了PFOS进入细胞的潜在途径，因为观察到PFOS与atp结合盒（ABC）转运体的底物结合蛋白结合，并与关键功能酶相互作用，从而抑制产甲烷菌。这项研究为全氟辛烷磺酸通过选择性抑制产甲烷古菌而不是通过普遍抑制产酸细菌来破坏碳代谢通量的分子阻断机制提供了新的见解。

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引用次数: 0

The role of artificial intelligence in biomass pyrolysis: Advancing predictive modelling and mechanistic understanding through machine learning − A comprehensive review 人工智能在生物质热解中的作用：通过机器学习推进预测建模和机制理解-综合综述

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-04 DOI: 10.1016/j.biortech.2025.133777

Luchen Yang , Brahim El Allaoui , Xinyun Wu , Tao Wu , Wai Siong Chai , Cheng Heng Pang

Biomass pyrolysis is a promising thermochemical pathway for producing renewable fuels and chemicals, yet its optimization remains challenging due to the complex interactions among feedstock properties, operating conditions, and reactor configurations. Artificial intelligence (AI), particularly machine learning (ML), is emerging as a powerful tool to improve predictive modelling and mechanistic understanding of pyrolysis. This review critically examines four pyrolysis regimes (slow, intermediate, fast, and flash) and advanced strategies such as co-pyrolysis, catalytic enhancement, and microwave heating, before discussing how ML algorithms are reshaping data-driven modelling. Supervised models, including random forests (RF), extreme gradient boosting (XGBoost), and artificial neural networks (ANNs), have achieved high accuracy in predicting product yields, while unsupervised and reinforcement learning approaches reveal hidden trends and enable adaptive process control. Furthermore, explainable AI frameworks (XAI), such as Shapley Additive Explanations (SHAP) and partial dependence plots (PDP), provide mechanistic insights that bridge data with reaction pathways. By combining AI with reactor optimization and mechanistic explanation, this field offers a pathway to efficient and scalable pyrolysis systems. Finally, we outline research gaps and future directions, emphasising the need for interpretable and generalisable models that can guide large-scale deployment.

生物质热解是一种很有前途的生产可再生燃料和化学品的热化学途径，但由于原料性质、操作条件和反应器配置之间复杂的相互作用，其优化仍然具有挑战性。人工智能（AI），特别是机器学习（ML），正在成为改进预测建模和对热解机理理解的有力工具。在讨论机器学习算法如何重塑数据驱动建模之前，本文对四种热解方式（慢速、中间、快速和快速）和高级策略（如共热解、催化增强和微波加热）进行了严格的研究。有监督模型，包括随机森林（RF）、极端梯度增强（XGBoost）和人工神经网络（ann），在预测产品产量方面取得了很高的准确性，而无监督和强化学习方法揭示了隐藏的趋势，并实现了自适应过程控制。此外，可解释的人工智能框架（XAI），如Shapley加性解释（SHAP）和部分依赖图（PDP），提供了将数据与反应途径联系起来的机制见解。通过将人工智能与反应器优化和机理解释相结合，该领域为高效、可扩展的热解系统提供了一条途径。最后，我们概述了研究差距和未来的方向，强调需要可解释和可推广的模型，以指导大规模部署。

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引用次数: 0

Untreated molasses efficiently Enhances 2,3-Butanediol synthesis using engineered Aureobasidium melanogenum P8AC-4 未经处理的糖蜜有效地提高了2,3-丁二醇的合成，利用工程的黑素短毛霉P8AC-4

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-04 DOI: 10.1016/j.biortech.2025.133776

Kai Wang , Xiancheng Wang , Enpeng Zhang , Haixia Yang , Qinggele Caiyin , Mingzhang Wen , Jianjun Qiao

2,3-Butanediol, an important platform chemical, possesses extensive applications in chemical engineering, pharmaceuticals, and materials science. To address the challenges of resource depletion and environmental impact associated with conventional petroleum-based synthesis, this study focuses on the sustainable production of 2,3-BDO via microbial fermentation. Aureobasidium melanogenum TSYW-58 was employed as the chassis for metabolic engineering. In previous work, genes responsible for the biosynthesis of pullulan, polymalic acid, melanin, and liamocin were deleted, yielding strain PPLM-8, characterized by high gluconic acid titer and reduced by-product formation. Building upon this platform, genes encoding α-acetolactate synthase and α-acetolactate decarboxylase from Bacillus subtilis were heterologously expressed, along with the endogenous butanediol dehydrogenase, leading to the successful construction of strain P8AC-4. This strain is capable of efficient 2,3-BDO production with minimal by-products. Shake-flask fermentation yielded 43.21 ± 1.23 g/L of 2,3-BDO, with a stereoisomer composition of 77.07 % meso- and 22.93 % (R,R)-isomer. Fermentation kinetic analysis in a 5 L bioreactor revealed that 2,3-BDO biosynthesis is tightly coupled with the logarithmic growth phase of the microorganism. By leveraging this metabolic trait, growth-inhibiting components present in untreated straw hydrolysate and molasses were employed to modulate fungal growth rates. Notably, cultivation with untreated molasses as the carbon source effectively extended the product synthesis phase, achieving a 2,3-BDO concentration of 110.37 ± 4.43 g/L in batch culture, representing a 155.42 % increase compared to that obtained with the glucose-based medium. These findings offer an efficient and cost-effective biomanufacturing strategy for the industrial-scale production of 2,3-BDO, contributing to the advancement of sustainable chemical synthesis.

2,3-丁二醇是一种重要的平台化学品，在化学工程、制药和材料科学等领域有着广泛的应用。为了解决与传统石油基合成相关的资源枯竭和环境影响的挑战，本研究侧重于通过微生物发酵可持续生产2,3- bdo。以黑素小孢子菌TSYW-58为基础进行代谢工程。在之前的研究中，我们删除了负责普鲁兰、聚苹果酸、黑色素和利亚霉素生物合成的基因，产生了菌株PPLM-8，其特点是葡萄糖酸滴度高，副产物形成减少。在此平台上，将枯草芽孢杆菌α-乙酰乳酸合成酶和α-乙酰乳酸脱羧酶基因与内源性丁二醇脱氢酶进行异源表达，成功构建菌株P8AC-4。该菌株能够以最少的副产物高效生产2,3- bdo。摇瓶发酵产2,3- bdo 43.21±1.23 g/L，立体异构体组成为77.07 %中位异构体和22.93 % （R，R）异构体。在5l生物反应器中发酵动力学分析表明，2,3- bdo的生物合成与微生物的对数生长阶段紧密耦合。利用这一代谢特性，未经处理的秸秆水解物和糖蜜中存在的生长抑制成分被用来调节真菌的生长速率。值得注意的是，以未经处理的糖蜜为碳源的培养有效地延长了产物合成阶段，在批量培养中达到2,3- bdo浓度为110.37±4.43 g/L，与葡萄糖基培养基相比，增加了155.42 %。这些发现为2,3- bdo的工业规模生产提供了一种高效且具有成本效益的生物制造策略，有助于推进可持续化学合成。

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引用次数: 0

Enhanced azithromycin removal in microalgae-microbial fuel cells: comparative analysis of anode and cathode configurations 微藻-微生物燃料电池对阿奇霉素的去除：阳极和阴极配置的比较分析。

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-03 DOI: 10.1016/j.biortech.2025.133771

Jesna Fathima , P.R. Sreekutty , Pritha Chatterjee

Azithromycin (AZ), a broad-spectrum antibiotic, is commonly found in aquatic habitats. This study investigated two microalgae-microbial fuel cell (m-MFC) configurations, A-AZ-MFC (AZ in the anode) and C-AZ-MFC (AZ in the cathode), which were run in fed-batch mode under open- and closed-circuit conditions (10–200 mg/L AZ). AZ removal increased from 43 % in an open circuit to 83 % when the circuit was closed in A-AZ-MFC and from 68 % to 84 % in C-AZ-MFC. While both A-AZ-MFC and C-AZ-MFC achieved comparable AZ degradation (83–84 %), A-AZ-MFC demonstrated superior electrochemical output (Power density: 275 mW/m³; Net energy recovery: 0.11 kWh/kg COD; Coulombic efficiency: 26 %) and higher microbial tolerance (IC₅₀ = 77.02 mg/L), indicating effective electron transfer and steady biofilm activity. Both designs achieved successful detoxification, as evidenced by comparable transformation product profiles and lower effluent toxicity. These findings demonstrate m-MFCs, especially anode-optimized systems, as sustainable platforms for the removal of antibiotics and the production of bioenergy.

阿奇霉素（AZ）是一种广谱抗生素，常见于水生生境。本研究研究了两种微藻-微生物燃料电池（m-MFC）配置，A-AZ-MFC（阳极AZ）和C-AZ-MFC（阴极AZ），在开路和闭路条件下（10-200 mg/L AZ）以进料间歇模式运行。在A-AZ-MFC中，AZ去除率从开路时的43 %增加到83 %，在C-AZ-MFC中从68 %增加到84 %。虽然A-AZ-MFC和C-AZ-MFC对AZ的降解效果相当（83-84 %），但A-AZ-MFC表现出更好的电化学输出（功率密度：275 mW/m3；净能量回收率：0.11 kWh/kg COD；库仑效率：26 %）和更高的微生物耐受性（IC50 = 77.02 mg/L），表明有效的电子转移和稳定的生物膜活性。两种设计都实现了成功的解毒，如可比的转化产品概况和较低的出水毒性所证明的那样。这些发现表明，m- mfc，特别是阳极优化系统，是去除抗生素和生产生物能源的可持续平台。

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引用次数: 0

Electrocoagulation-enhanced pretreatment for efficient operation of packed bed anaerobic filter treating leachate: kinetic and performance evaluation 填料床厌氧滤池处理渗滤液高效运行的电混凝强化预处理：动力学与性能评价。

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-03 DOI: 10.1016/j.biortech.2025.133770

Ashmita Kundu, Ajay S. Kalamdhad

A study was carried out on the sequential treatment of young high-strength landfill leachate using an aluminium electrode in a batch electrochemical cell reactor as a pretreatment, followed by a packed-bed upflow anaerobic filter (PBUAF) as a treatment method. Two landfill-simulating reactors, made of iron sheets having sizes of 1 m × 1 m × 1.1 m (Length × Width × Height), were operated under varying hydrological conditions where one was operated without rain (S1) and another with simulated rain (S2). The reactors were loaded with 450 kg of municipal solid waste, comprised of 51 % wet and 49 % dry fractions, typical of Indian waste. Electrocoagulation was performed as a pretreatment process with aluminium electrodes for 10 min, and it achieved 40–50 % removal efficiency, which preconditioned the leachate to be treated further by biological means. The pretreated leachate, supplemented with de-oiled rice bran (DORB), was introduced to the PBUAF. The PBUAF was able to achieve substantial pollutant removal, attaining 93.9 % of Chemical Oxygen Demand (COD), 97.46 % of Total Organic Carbon (TOC), and 94.24 % of Total Kjeldahl Nitrogen (TKN), in addition to other recalcitrant pollutants at an optimized organic loading rate (OLR) of 5.107 g COD/L/day and a hydraulic retention time of 8 days. Kinetic analysis indicated that the Stover–Kincannon and Monod models were highly consistent with experimental data. The Electrocoagulation-PBUAF hybrid system effectively removed persistent organic pollutants, including naphthalene, phenols, benzene derivatives, halogenated compounds, alcohols, and phthalates, demonstrating its capacity to treat both prevalent and resistant pollutants efficiently.

采用铝电极在间歇电化学电池反应器中进行预处理，然后采用填料床上流式厌氧过滤器（PBUAF）进行处理，研究了新型高强度垃圾渗滤液的顺序处理方法。两个landfill-simulating反应堆,由铁皮有大小1 m × 1 m × 1.1 米(长度 × 宽度 × 高度),在不同水文条件下操作,一个操作没有雨(S1)和另一个模拟雨(S2)。反应器装载了450 公斤的城市固体废物，包括51 %湿馏分和49 %干馏分，这是典型的印度废物。铝电极电絮凝预处理时间为10 min，去除率达到40-50 %，为进一步生物处理渗滤液做好了准备。将预处理后的渗滤液添加去油米糠（DORB），引入PBUAF。PBUAF在优化的有机负荷率（OLR）为5.107 g COD/L/天，水力停留时间为8 天的情况下，除其他难降解污染物外，PBUAF的化学需氧量（COD）去除率为93.9 %，总有机碳（TOC）去除率为97.46 %，总凯氏氮（TKN）去除率为94.24 %。动力学分析表明，Stover-Kincannon和Monod模型与实验数据高度一致。电混凝- pbuaf混合系统有效去除持久性有机污染物，包括萘、酚类、苯衍生物、卤化化合物、醇类和邻苯二甲酸盐，证明了其有效处理常见和抗性污染物的能力。

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引用次数: 0

Upcycling eucalyptus kraft lignin into fluorescent graphene quantum dots for reproductive cell imaging in aquaculture biotechnology. 将桉树硫酸盐木质素升级为荧光石墨烯量子点，用于水产生物技术中的生殖细胞成像。

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-03 DOI: 10.1016/j.biortech.2025.133769

Anna Octavera, Harits Atika Ariyanta, Khanesisya Luthfiyah Zahra, Diana Faoziah Hakim, Fajar Maulana, Novia Amalia Sholeha, Dian Hardiantho, Nurlatiha Khairun Nisa, Widya Fatriasari, Alimuddin

The Kraft pulping industry produces significant amounts of lignin-rich black liquor, a largely underutilized renewable resource. We report a scalable method to synthesize lignin-derived graphene quantum dots from raw eucalyptus kraft black liquor (EL-GQDs) through sequential acid oxidation and hydrothermal carbonization. Structural and surface analyses confirm the formation of graphitic nanostructures with oxygen- and nitrogen-rich functional groups, exhibiting strong visible fluorescence, good aqueous dispersibility, and high photostability, with uniform morphology and lattice fringes characteristic of crystalline GQDs. The bioimaging potential of EL-GQDs was tested using Nile tilapia testicular cells in vitro and in vivo, achieving efficient cell labeling with low toxicity. Labeled cells remained viable and localized within the gonads up to 30 days post-transplantation. These results demonstrate that EL-GQDs are biocompatible, photostable, and sustainable nanoprobes suitable for long-term cell tracking in aquatic reproductive research. Further studies on uptake pathways, cross-species application, and long-term biosafety are needed to advance this technology.

卡夫制浆工业生产大量的富含木质素的黑液，这是一种未充分利用的可再生资源。我们报道了一种可扩展的方法，通过连续酸氧化和水热碳化，以桉木硫酸盐黑液（EL-GQDs）为原料合成木质素衍生的石墨烯量子点。结构和表面分析证实了石墨纳米结构的形成，具有富氧和富氮官能团，具有强的可见荧光，良好的水分散性和高的光稳定性，具有晶体GQDs的均匀形态和晶格条纹特征。利用尼罗罗非鱼睾丸细胞在体内和体外测试了EL-GQDs的生物成像潜力，实现了低毒性的高效细胞标记。标记的细胞在移植后30 天内仍能存活并定位在性腺内。这些结果表明，EL-GQDs是一种生物相容性、光稳定性和可持续性的纳米探针，适合用于水生生殖研究中的长期细胞跟踪。需要进一步研究其吸收途径、跨物种应用和长期生物安全性。

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引用次数: 0

Using the hydrolysate of Chinese medicine residues as nutrient source improves the performance of oil-producing algae: Phenomena and mechanistic elucidation. 以中药残渣水解液为营养源可提高产油藻类的生产性能：现象及机理解析。

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-03 DOI: 10.1016/j.biortech.2025.133737

Shiyi Chen, Ruixin Guo, Zixuan Zheng, Wenling Chen, Cheng Ma, Yanhua Liu, Jianqiu Chen, Jian Ke

The development of microalgae-based biofuels has long been a research focus for achieving green and sustainable development. Chinese medicine residues, an abundant organic waste rich in cellulose, hemicellulose, lignin, proteins, polysaccharides, and microminerals, offer scalable feedstock. Here, their hydrolysate (HCMR) was used as an additional nutrient source for cultivating oil-producing algae. The hydrolysate promotes the growth and productivity of oil-producing algae such as Chlorococcum sp., Tribonema aequale, and Scenedesmus sp., while also indicating its feasibility for scaling up Scenedesmus sp. cultivation. The HCMR addition increased biomass by 1.58-fold and fatty‑acid content by 1.27‑fold relative to BG11 medium. Analysis of the fatty acid composition in Scenedesmus sp. confirmed that the addition of hydrolysate from Chinese medicine residues significantly increased the proportions of high-quality fatty acids, including palmitoleic acid (C16:1) and oleic acid (C18:1), while reducing the proportions of linoleic acid (C18:2) and α-linolenic acid (C18:3), thereby improving the cetane number and oxidative stability of the biodiesel. Metabolomics analysis reveals potential regulatory pathways by which HCMR enhances lipid accumulation in Scenedesmus sp., implicating transamination in glutamate metabolism and offering a sustainable route for utilizing similar organic waste as an additional source of nutrients in oil-producing microalgae cultivation.

微藻生物燃料的开发一直是实现绿色可持续发展的研究热点。中药残渣是一种富含纤维素、半纤维素、木质素、蛋白质、多糖和微量矿物质的有机废物，是可扩展的原料。在这里，它们的水解物（HCMR）被用作培养产油藻类的额外营养来源。该水解液对产油藻类如chlorcoccum sp.、Tribonema aequale和Scenedesmus sp.的生长和产量有促进作用，同时也表明了扩大Scenedesmus sp.养殖的可行性。与BG11培养基相比，添加HCMR可使生物量提高1.58倍，脂肪酸含量提高1.27倍。通过对Scenedesmus sp.脂肪酸组成的分析，证实中药残液水解液的加入显著提高了棕榈油酸（C16:1）和油酸（C18:1）等优质脂肪酸的比例，同时降低了亚油酸（C18:2）和α-亚麻酸（C18:3）的比例，从而提高了十六烷值和氧化稳定性。代谢组学分析揭示了HCMR促进Scenedesmus sp.脂质积累的潜在调控途径，暗示谷氨酸代谢中的转氨酶作用，并为利用类似的有机废物作为产油微藻培养的额外营养来源提供了可持续的途径。

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引用次数: 0

Regulation of antibiotic resistance genes dissemination by in situ formed iron sulfide nanoparticles in anaerobic microbial communities 原位形成的硫化铁纳米颗粒在厌氧微生物群落中对抗生素抗性基因传播的调控。

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-03 DOI: 10.1016/j.biortech.2025.133773

Yaru Zhang , WanKai Ma , Bobing Lu , Hui Wang , Qiang Zhang , Zhaoyong Bian

The aim of this study was to investigate the mechanisms of in situ-synthesized iron sulfide nanoparticles (bio-FeS NPs) on the dissemination of antibiotic resistance genes (ARGs) in anaerobic microbial consortia. The absolute abundance of plasmid RP4-associated extracellular ARGs (eARGs) decreased by more than 10³ copies/ng DNA in the anaerobic consortia containing in situ-formed bio-FeS NPs (AnBR-FeS NPs). Mechanistic analysis revealed that the inhibitory effect was related to the absence of a significant correlation between functional bacteria such as Nitratidesulfovibrio in AnBR-FeS NPs and Escherichia carrying the RP4 plasmid, as well as a 2.21-fold increase in calcium/magnesium adenosine triphosphatase and a 1.86-fold reduction in reactive oxygen species levels. Notably, co-occurrence analysis indicated that bio-FeS NPs preferentially maintained cellular homeostasis, reducing the dependence of ARG propagation on mobile genetic elements. This study provides new insights into the role of in situ nanominerals in suppressing ARG dissemination in anaerobic wastewater treatment systems.

本研究旨在探讨原位合成的硫化铁纳米颗粒（biofes NPs）在厌氧菌群中抗生素耐药基因（ARGs）传播的机制。在含有原位形成的生物fes NPs （AnBR-FeS NPs）的厌氧菌群中，质粒rp4相关的胞外ARGs （eARGs）的绝对丰度降低了103拷贝/ng DNA以上。机制分析表明，其抑制作用与AnBR-FeS NPs中硝基脱硫弧菌等功能菌与携带RP4质粒的埃希氏菌之间不存在显著相关性，以及钙/镁腺苷三磷酸酶增加2.21倍，活性氧减少1.86倍有关。值得注意的是，共发生分析表明，生物fes NPs优先维持细胞稳态，降低了ARG繁殖对移动遗传元件的依赖。该研究为原位纳米矿物在抑制厌氧废水处理系统中ARG扩散中的作用提供了新的见解。

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引用次数: 0

Stress-tolerant heterotrophic nitrification-aerobic denitrification strains: nitrogen removal performance, applications, and mechanisms. 耐应力异养硝化-好氧反硝化菌株：脱氮性能、应用和机制。

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-03 DOI: 10.1016/j.biortech.2025.133768

Heng Wu, Guoqiang Zhan, Huaiwen Zhang, Xuanmin Yang

Stress-resistant heterotrophic nitrification-aerobic denitrification (HN-AD) strains can efficiently remove nitrogen from wastewater with high ammonia, high acidity, high salinity, low temperature, and heavy metals. This study systematically reviews the nitrogen removal characteristics, application performance, metabolic mechanisms and future prospects of stress-resistant HN-AD strains. As a result, (1) Stress-resistant HN-AD strains have gained growing interest, especially multi-stress-resistant strains. (2) HN-AD genera Acinetobacter (resistant to high ammonia and low temperature) and Pseudomonas (resistant to high ammonia and salinity) can improve bioreactors' tolerance, requiring further engineering applications. (3) Stress-resistant HN-AD strains resist stress via three mechanisms: upregulating nitrogen metabolism genes, enhancing membrane transport, and modulating signaling molecules, which results in enhanced bioreactor performance. (4) Screening multi-stress-resistant strains should remain a priority, focusing on large-scale application, conductive material immobilization, colonization and genetic mechanism verification, and life cycle/economic analysis. This study provides vital references for HN-AD strain screening and applications under extreme conditions.

抗逆性异养硝化-好氧反硝化（HN-AD）菌株对高氨、高酸性、高盐度、低温、重金属废水的脱氮效果显著。本文系统综述了抗逆性HN-AD菌株的脱氮特性、应用性能、代谢机制及未来发展前景。因此，(1)抗逆性强的HN-AD菌株受到越来越多的关注，特别是多重抗逆性菌株。(2) HN-AD属不动杆菌（耐高氨和低温）和假单胞菌（耐高氨和盐度）可以提高生物反应器的耐受性，需要进一步的工程应用。(3)抗逆性HN-AD菌株通过上调氮代谢基因、增强膜转运和调节信号分子三种机制抵抗胁迫，从而提高生物反应器性能。(4)筛选多抗逆性菌株仍应优先考虑，重点是大规模应用、导电材料固定化、定植和遗传机制验证以及生命周期/经济分析。本研究为HN-AD菌株的筛选及在极端条件下的应用提供了重要参考。

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引用次数: 0

Integrated production to digestion of a mycelium lignocellulosic biocomposite: Effects of temperature and co-digestion 菌丝体木质纤维素生物复合材料的综合生产与消化：温度和共消化的影响。

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-03 DOI: 10.1016/j.biortech.2025.133772

Seongbong Heo , Jihea Lee , Woo Jin Nam , Suhyun Kim , Kyeong Hwan Kang , Jong-Rok Jeon , Young Mo Kim

The current study developed a mycelium lignocellulosic biocomposite (MLB) from rice husk and tangerine peel and evaluated its potential for resource recovery through anaerobic digestion (AD) under different temperatures. The MLB was cultivated with G. lucidum without the use of synthetic binders. Several substrate-to-water ratios were examined, with a 1:2 ratio yielding the most favorable material properties. After production, the MLB was subjected to AD at 36 °C and 55 °C using two operational strategies: mono-digestion and co-digestion with sewage sludge (SL). Under mesophilic co-digestion, methane yields reached 284 mL CH₄/g∙VS_feed, accompanied by efficient conversion of volatile fatty acids (VFAs) to methane. However, thermophilic operation enriched heat-adapted consortia but led to VFA accumulation and reduced methane production. These results highlight a substrate-dependent temperature effect: thermophilic conditions enhance sludge biodegradation but do not sufficiently overcome the recalcitrance of MLB, whereas mesophilic co-digestion promotes syntrophic interactions and achieves higher conversion to methane.

本研究以稻壳和陈皮为原料制备了一种木质纤维素生物复合材料（MLB），并对其在不同温度下通过厌氧消化（AD）回收的潜力进行了评价。用灵芝培养MLB，不使用合成黏合剂。测试了几种基材与水的比例，其中1:2的比例产生最有利的材料性能。生产后，MLB在36 °C和55 °C的条件下进行AD处理，采用两种操作策略：单消化和与污水污泥（SL）共消化。在中温共消化条件下，甲烷产率达到284 mL CH4/g∙VSfeed，挥发性脂肪酸（VFAs）高效转化为甲烷。然而，嗜热操作丰富了热适应菌群，但导致VFA积累和甲烷产量降低。这些结果强调了一个依赖于底物的温度效应：嗜热条件增强了污泥的生物降解，但不能充分克服MLB的顽固性，而中温共生消化促进了共生相互作用，并实现了更高的甲烷转化。

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