Pub Date : 2025-04-10DOI: 10.1016/j.biortech.2025.132528
Ulises Andrés Salas-Villalobos , Diego Homma-Dueñas , Mario A. Torres-Acosta , Oscar Aguilar
The demand for natural and safe products has fueled interest in molecules with bioactive properties and low toxicity. Prodigiosin, a red alkaloid produced by various bacterial strains, has gained attention for its antimicrobial and antitumor properties. Traditional production methods often face challenges such as inhibition by the product and high costs. This study explores multistage continuous reactors (MSCR) for competitive prodigiosin production via extractive fermentation. A factorial design was conducted to evaluate dilution rates (0.042 – 0.050 h−1) and mixed reactor volume configurations (1 L – 0.5 L). The optimal system was modeled using kinetic equations, and its economic viability was assessed at different dilution rates. Results demonstrate that the separation of growth and production/extraction stages improved productivity by 30 % and reduced costs by 20 % compared to a single continuous system. These findings suggest that MSCR, particularly in extractive fermentations, holds promise for intensifying prodigiosin production, leading to more economically competitive bioprocesses.
{"title":"Intensification and technoeconomic assessment of prodigiosin extractive fermentation using multistage continuous fermentation","authors":"Ulises Andrés Salas-Villalobos , Diego Homma-Dueñas , Mario A. Torres-Acosta , Oscar Aguilar","doi":"10.1016/j.biortech.2025.132528","DOIUrl":"10.1016/j.biortech.2025.132528","url":null,"abstract":"<div><div>The demand for natural and safe products has fueled interest in molecules with bioactive properties and low toxicity. Prodigiosin, a red alkaloid produced by various bacterial strains, has gained attention for its antimicrobial and antitumor properties. Traditional production methods often face challenges such as inhibition by the product and high costs. This study explores multistage continuous reactors (MSCR) for competitive prodigiosin production via extractive fermentation. A factorial design was conducted to evaluate dilution rates (0.042 – 0.050 h<sup>−1</sup>) and mixed reactor volume configurations (1 L – 0.5 L). The optimal system was modeled using kinetic equations, and its economic viability was assessed at different dilution rates. Results demonstrate that the separation of growth and production/extraction stages improved productivity by 30 % and reduced costs by 20 % compared to a single continuous system. These findings suggest that MSCR, particularly in extractive fermentations, holds promise for intensifying prodigiosin production, leading to more economically competitive bioprocesses.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132528"},"PeriodicalIF":9.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-10DOI: 10.1016/j.biortech.2025.132503
Sifang Wang , Shu Wang , Kaoming Zhang , Zerong Jiang , Ziyao Chen , Yu Miao , Kailong Huang , Chun Hu , Zhu Wang
Sulfamethoxazole (SMX) present in pharmaceutical wastewater may pose significant risks to ecological health. This study evaluated the role of redox mediator nano Fe3O4-modified graphene (GF) to facilitate SMX biotransformation in a sulfate reduction reactor (Rs) and an anaerobic digestion reactor (Ra). The results revealed that the SMX removal in Rs and Ra after GF addition reached 92% and 97%, respectively. By stimulating the secretion of humus-like substances (containing quinone group), riboflavin, and conductive proteins, GF enhanced direct interspecies electron transfer (DIET) among microorganisms in both Rs and Ra. Additionally, in both systems, the relative abundance of genes encoding cytochrome c oxidase and type IV pilus assembly proteins decreased. These metabolic shifts reduced the reliance of DIET on cytochrome c and ciliates while enhancing energy utilization. The results confirmed that GF can serve as an effective additive for enhancing SMX degradation in anaerobic systems.
{"title":"Nano Fe3O4-modified graphene enhancing the removal of sulfamethoxazole under anaerobic digestion and sulfate reduction conditions through improved direct interspecies electron transfer","authors":"Sifang Wang , Shu Wang , Kaoming Zhang , Zerong Jiang , Ziyao Chen , Yu Miao , Kailong Huang , Chun Hu , Zhu Wang","doi":"10.1016/j.biortech.2025.132503","DOIUrl":"10.1016/j.biortech.2025.132503","url":null,"abstract":"<div><div>Sulfamethoxazole (SMX) present in pharmaceutical wastewater may pose significant risks to ecological health. This study evaluated the role of redox mediator nano Fe<sub>3</sub>O<sub>4</sub>-modified graphene (GF) to facilitate SMX biotransformation in a sulfate reduction reactor (Rs) and an anaerobic digestion reactor (Ra). The results revealed that the SMX removal in Rs and Ra after GF addition reached 92% and 97%, respectively. By stimulating the secretion of humus-like substances (containing quinone group), riboflavin, and conductive proteins, GF enhanced direct interspecies electron transfer (DIET) among microorganisms in both Rs and Ra. Additionally, in both systems, the relative abundance of genes encoding cytochrome <em>c</em> oxidase and type IV pilus assembly proteins decreased. These metabolic shifts reduced the reliance of DIET on cytochrome <em>c</em> and ciliates while enhancing energy utilization. The results confirmed that GF can serve as an effective additive for enhancing SMX degradation in anaerobic systems.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132503"},"PeriodicalIF":9.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A modified fruit blender reactor (M−FBR) was applied to synthesize monoacylglycerols (MAGs) and diacylglycerols (DAGs) through biphasic esterification of glycerol (Gly) with oleic acid (OA). The high stirring speed in the M−FBR provided a high mixing efficiency to generate significantly small droplets and cavitation compared to those in conventional processes. The increased interfacial surface area and distribution of dispersed phases into continuous phases promote momentum, heat, and mass transfer to improve mixing efficiency and high glycerol esterification rate. The reaction time and methanesulfonic acid (MSA) concentration were the most significant factors, affecting both OA conversion and MAG-DAG yields, while the Gly/OA molar ratio exhibited a less significant effect. The highest yields for MAG-DAG in 60 min using the M−FBR were 80.4 and 82.9% at 110 and 130 °C, respectively. The values of yield efficiency of M−FBR were 67.3 x 10−4 g/J at 110 °C and 69.8 x 10−4 g/J at 130 °C, which were up to 25–fold higher than that of the mechanical stirred reactor. The M−FBR generated small glycerol droplet (46–56 µm) to enhance the glycerol solubility in the OA phase, allowing the MAG and DAG synthesis to be conducted at a lower Gly/OA molar ratio. This offered higher selectivities of MAG and DAG and prevent undesirable TAG. Additionally, the simplified evolution of MAG-DAG formation during the glycerol esterification of OA was elucidated by ATR-FTIR spectroscopy.
{"title":"Intensified modified fruit blender reactor for emulsifier synthesis via glycerol esterification of free fatty acids at mild conditions","authors":"Narita Chanthon , Kanokwan Ngaosuwan , Worapon Kiatkittipong , Doonyapong Wongsawaeng , Weerinda Mens , Nopphon Weeranoppanant , Apinan Soottitantawat , Pongtorn Charoensuppanimit , Samuel Lalthazuala Rokhum , Suttichai Assabumrungrat","doi":"10.1016/j.biortech.2025.132510","DOIUrl":"10.1016/j.biortech.2025.132510","url":null,"abstract":"<div><div>A modified fruit blender reactor (M−FBR) was applied to synthesize monoacylglycerols (MAGs) and diacylglycerols (DAGs) through biphasic esterification of glycerol (Gly) with oleic acid (OA). The high stirring speed in the M−FBR provided a high mixing efficiency to generate significantly small droplets and cavitation compared to those in conventional processes. The increased interfacial surface area and distribution of dispersed phases into continuous phases promote momentum, heat, and mass transfer to improve mixing efficiency and high glycerol esterification rate. The reaction time and methanesulfonic acid (MSA) concentration were the most significant factors, affecting both OA conversion and MAG-DAG yields, while the Gly/OA molar ratio exhibited a less significant effect. The highest yields for MAG-DAG in 60 min using the M−FBR were 80.4 and 82.9% at 110 and 130 °C, respectively. The values of yield efficiency of M−FBR were 67.3 x 10<sup>−4</sup> g/J at 110 °C and 69.8 x 10<sup>−4</sup> g/J at 130 °C, which were up to 25–fold higher than that of the mechanical stirred reactor. The M−FBR generated small glycerol droplet (46–56 µm) to enhance the glycerol solubility in the OA phase, allowing the MAG and DAG synthesis to be conducted at a lower Gly/OA molar ratio. This offered higher selectivities of MAG and DAG and prevent undesirable TAG. Additionally, the simplified evolution of MAG-DAG formation during the glycerol esterification of OA was elucidated by ATR-FTIR spectroscopy.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132510"},"PeriodicalIF":9.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hydrothermal carbonization facilitates microalgae utilization but suffers from low product quality, with no effective solutions. This study investigated the product characteristics and quality enhancement methods of hydrochar, bio-oil, and carbon dots prepared through hydrothermal carbonization of microalgae assisted by non-polar organic solvents (petroleum ether and n-hexane) and polar organic solvents (ethyl acetate, isopropanol, and ethanol). The results showed that non-polar organic solvents were more effective than polar organic solvents in extracting fatty acids hydrolyzed from lipids into the organic phase, reducing amidation with amino acids and lowering bio-oil nitrogen content by 47.0%, with n-hexane exhibiting the best effect. They also promoted Maillard reactions, increasing hydrochar and carbon dots yields by 41.2% and 63.3% while boosting nitrogen content by 48.9% and 15.7%, respectively. This study explored the co-production and upgrading of multiple products in microalgae hydrothermal carbonization with organic solvent assistance, offering insights into process optimization for efficient utilization of microalgae.
{"title":"Co-production and upgrading of multiple products from hydrothermal carbonization of microalgae with organic solvent assistance","authors":"Bin Zhang , Jingmiao Zhang , Ao Xia , Xinru Tang , Xianqing Zhu , Yun Huang , Xun Zhu , Qiang Liao","doi":"10.1016/j.biortech.2025.132514","DOIUrl":"10.1016/j.biortech.2025.132514","url":null,"abstract":"<div><div>Hydrothermal carbonization facilitates microalgae utilization but suffers from low product quality, with no effective solutions. This study investigated the product characteristics and quality enhancement methods of hydrochar, bio-oil, and carbon dots prepared through hydrothermal carbonization of microalgae assisted by non-polar organic solvents (petroleum ether and <em>n</em>-hexane) and polar organic solvents (ethyl acetate, isopropanol, and ethanol). The results showed that non-polar organic solvents were more effective than polar organic solvents in extracting fatty acids hydrolyzed from lipids into the organic phase, reducing amidation with amino acids and lowering bio-oil nitrogen content by 47.0%, with <em>n</em>-hexane exhibiting the best effect. They also promoted Maillard reactions, increasing hydrochar and carbon dots yields by 41.2% and 63.3% while boosting nitrogen content by 48.9% and 15.7%, respectively. This study explored the co-production and upgrading of multiple products in microalgae hydrothermal carbonization with organic solvent assistance, offering insights into process optimization for efficient utilization of microalgae.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132514"},"PeriodicalIF":9.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biomass is the most plentiful renewable source of energy. Biomass has many uses, one of which is the production of activated carbon (AC), which is then utilised as an electrode in energy storage devices. The goal of this work is to synthesize a novel AC that is compatible with room temperature triazolium-based ionic liquids. The prepared AC was characterized by BET, SEM, XRD, and RAMAN techniques. The surface area, pore volume, and pore radius of the AC were 780 m2/g, 0.519 cc/g, and 1.33 nm, respectively. Additionally, novel symmetrically and asymmetrically substituted triazolium ionic liquids (IL) are synthesized and characterized. Cyclic voltammetry, electrochemical impedance spectroscopy, and galvanostatic charge–discharge studies were used to examine the electrochemical performance of the device. The symmetrical IL provided an excellent specific capacitance of 62.29F/g at 2 mV/s, an energy density of 1.06 W h/kg, a power density of 229.5 W/kg, and maintained 76 % retention after 1500 cycles. For asymmetrically substituted IL, a reversible potential window of around 2 V was obtained along with a capacitance of 91.31F/g at 2 mV/s, an energy density of 2.8 W h/kg, a power density of 420 W/kg, and maintained 73.9 % of capacitance after 1500 cycles.
生物质是最丰富的可再生能源。生物质有许多用途，其中之一是生产活性炭（AC），然后将其用作储能设备的电极。这项工作的目标是合成一种新型活性炭，它与室温三唑基离子液体兼容。制备的交流电通过 BET、SEM、XRD 和 RAMAN 技术进行表征。交流电的表面积、孔体积和孔半径分别为 780 m2/g、0.519 cc/g 和 1.33 nm。此外，还合成并表征了新型对称和不对称取代的三唑离子液体（IL）。使用循环伏安法、电化学阻抗光谱法和电静态充放电研究来检验该装置的电化学性能。对称IL在2 mV/s条件下的比电容为62.29F/g，能量密度为1.06 W h/kg，功率密度为229.5 W/kg，循环1500次后仍能保持76%的电容量。对于不对称取代的 IL，获得了约 2 V 的可逆电位窗口，在 2 mV/s 时电容为 91.31F/g，能量密度为 2.8 W h/kg，功率密度为 420 W/kg，1500 次循环后电容保持率为 73.9%。
{"title":"Sustainable activated carbon derived from biomass of Borassus flabellifer: Unveiling their potential as electrode in supercapacitors for triazolium ionic liquid-based systems","authors":"Chaithanya Vijay , E.P. Abhijith , Sushmita Sushil , Anjitha Satheesh , Elango Kandasamy","doi":"10.1016/j.biortech.2025.132520","DOIUrl":"10.1016/j.biortech.2025.132520","url":null,"abstract":"<div><div>Biomass is the most plentiful renewable source of energy. Biomass has many uses, one of which is the production of activated carbon (AC), which is then utilised as an electrode in energy storage devices. The goal of this work is to synthesize a novel AC that is compatible with room temperature triazolium-based ionic liquids. The prepared AC was characterized by BET, SEM, XRD, and RAMAN techniques. The surface area, pore volume, and pore radius of the AC were 780 m<sup>2</sup>/g, 0.519 cc/g, and 1.33 nm, respectively. Additionally, novel symmetrically and asymmetrically substituted triazolium ionic liquids (IL) are synthesized and characterized. Cyclic voltammetry, electrochemical impedance spectroscopy, and galvanostatic charge–discharge studies were used to examine the electrochemical performance of the device. The symmetrical IL provided an excellent specific capacitance of 62.29F/g at 2 mV/s, an energy density of 1.06 W h/kg, a power density of 229.5 W/kg, and maintained 76 % retention after 1500 cycles. For asymmetrically substituted IL, a reversible potential window of around 2 V was obtained along with a capacitance of 91.31F/g at 2 mV/s, an energy density of 2.8 W h/kg, a power density of 420 W/kg, and maintained 73.9 % of capacitance after 1500 cycles.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132520"},"PeriodicalIF":9.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-08DOI: 10.1016/j.biortech.2025.132507
Siyao Dai , Weiying Feng , Fanhao Song , Tingting Li , Yingru Tao , Fang Yang , Qingfeng Miao , Pengcheng Duan , Haiqing Liao , Haibin Shi , José Manuel Gonçalves , Isabel Maria Duarte
Periphyton-based biofertilizer have a high potential for soil remediation, particularly for controlling soil salinization. This global environmental problem leads to low soil utilization and insufficient crop yields. Efficient and sustainable methods of managing saline soils are needed to reduce salinization and improve soil fertility and crop quality. Traditional methods such as physical mulching and chemical amendments, while improving soil conditions, exhibit limited effectiveness and may damage soil structure. This study aims to evaluate the feasibility of algae-based fertilizers in remediating saline-alkali soils and improving crop performance. The review delves into the and application prospects of algae-based fertilizers, highlighting their potential from both sustainable development and economic perspectives. It further advocates integrating other emerging technologies with the production and application of algae-based fertilizers to address the increasingly severe challenges posed by degraded soil resources and environmental instability. The review found that algal fertilizers are more environmentally friendly than traditional chemical fertilizers but are not inferior in function. This approach offers more efficient and sustainable solutions for managing saline-alkaline soils and effectively achieves sustainable agricultural production. Furthermore, it is necessary to conduct experimental research and monitoring evaluations of algal fertilizers to formulate scientific and rational fertilization plans to meet the increasingly serious challenges facing soil resources and unstable environments. The findings of this study will provide theoretical and technical support for using algae biofertilizers for soil remediation, improving crop quality and sequestering carbon.
{"title":"Review of biological algal fertilizer technology: Alleviating salinization, sequestering carbon, and improving crop productivity","authors":"Siyao Dai , Weiying Feng , Fanhao Song , Tingting Li , Yingru Tao , Fang Yang , Qingfeng Miao , Pengcheng Duan , Haiqing Liao , Haibin Shi , José Manuel Gonçalves , Isabel Maria Duarte","doi":"10.1016/j.biortech.2025.132507","DOIUrl":"10.1016/j.biortech.2025.132507","url":null,"abstract":"<div><div>Periphyton-based biofertilizer have a high potential for soil remediation, particularly for controlling soil salinization. This global environmental problem leads to low soil utilization and insufficient crop yields. Efficient and sustainable methods of managing saline soils are needed to reduce salinization and improve soil fertility and crop quality. Traditional methods such as physical mulching and chemical amendments, while improving soil conditions, exhibit limited effectiveness and may damage soil structure. This study aims to evaluate the feasibility of algae-based fertilizers in remediating saline-alkali soils and improving crop performance. The review delves into the and application prospects of algae-based fertilizers, highlighting their potential from both sustainable development and economic perspectives. It further advocates integrating other emerging technologies with the production and application of algae-based fertilizers to address the increasingly severe challenges posed by degraded soil resources and environmental instability. The review found that algal fertilizers are more environmentally friendly than traditional chemical fertilizers but are not inferior in function. This approach offers more efficient and sustainable solutions for managing saline-alkaline soils and effectively achieves sustainable agricultural production. Furthermore, it is necessary to conduct experimental research and monitoring evaluations of algal fertilizers to formulate scientific and rational fertilization plans to meet the increasingly serious challenges facing soil resources and unstable environments. The findings of this study will provide theoretical and technical support for using algae biofertilizers for soil remediation, improving crop quality and sequestering carbon.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132507"},"PeriodicalIF":9.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-08DOI: 10.1016/j.biortech.2025.132504
Xuekang Cao , Yinguang Chen , Hao Zheng , Yong Liao , Lihua Feng , Jiacheng Feng , Chao Liu , Fangying Ji
Constructed wetlands (CWs) are widely used for nitrogen pollution control in rural aquatic environments, yet their nitrogen removal efficiency often remains suboptimal. This study firstly examines how zeolite robustly stimulates Fe-utilization of steelmaking waste (i.e., steel slag) to improve nitrification and autotrophic denitrification of low carbon-to-nitrogen (C/N) ratio wastewater (C/N ≈ 1). Steel slag, by providing alkalinity for nitrification, also serves as an electron donor for denitrification due to its low-valent iron content. As a result, the total nitrogen (TN) removal efficiency was increased by 153.5% compared to the control group. Zeolite reshaped the microbial consortia, enriching iron autotrophic denitrifying bacteria and aerobic denitrifying bacteria. More importantly, zeolite facilitated microbial iron utilization by enhancing transmembrane iron transport and intracellular iron oxidation to boost nitrification and autotrophic denitrification without additional aeration, external carbon sources, or pH regulation. Our work advances understanding the development of low carbon technologies for wastewater nitrogen removal.
{"title":"Integration of steel slag and zeolite enhances simultaneous nitrification and autotrophic denitrification in ultra-low carbon/nitrogen ratio wastewater: Remodeling microbiota and iron metabolism","authors":"Xuekang Cao , Yinguang Chen , Hao Zheng , Yong Liao , Lihua Feng , Jiacheng Feng , Chao Liu , Fangying Ji","doi":"10.1016/j.biortech.2025.132504","DOIUrl":"10.1016/j.biortech.2025.132504","url":null,"abstract":"<div><div>Constructed wetlands (CWs) are widely used for nitrogen pollution control in rural aquatic environments, yet their nitrogen removal efficiency often remains suboptimal. This study firstly examines how zeolite robustly stimulates Fe-utilization of steelmaking waste (<em>i.e.</em>, steel slag) to improve nitrification and autotrophic denitrification of low carbon-to-nitrogen (C/N) ratio wastewater (C/N ≈ 1). Steel slag, by providing alkalinity for nitrification, also serves as an electron donor for denitrification due to its low-valent iron content. As a result, the total nitrogen (TN) removal efficiency was increased by 153.5% compared to the control group. Zeolite reshaped the microbial consortia, enriching iron autotrophic denitrifying bacteria and aerobic denitrifying bacteria. More importantly, zeolite facilitated microbial iron utilization by enhancing transmembrane iron transport and intracellular iron oxidation to boost nitrification and autotrophic denitrification without additional aeration, external carbon sources, or pH regulation. Our work advances understanding the development of low carbon technologies for wastewater nitrogen removal.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132504"},"PeriodicalIF":9.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-08DOI: 10.1016/j.biortech.2025.132511
Pengyu Hu , Yunchuan Dai , Tingting Chu , Jiliang Ma , Xing Wang , Zhiwei Wang , Pedram Fatehi , Yanzhu Guo
The currently reported carbon dots with oxidase-like activity, as substitutes for natural enzymes, are generally prepared using organics with small molecules as carbon precursors. However, renewable cellulose as a feedstock is more conducive to the sustainable production of carbon dots. Herein, the Mn and N elements doped carbon dots with oxidase-like activity were prepared from carboxymethylcellulose, ethylenediamine, and MnCl2, and the effects of these three elements on the structure of the carbon dots were comprehensively studied and verified by advanced techniques. Moreover, the increase of ethylenediamine dosage and the prolongation of hydrothermal time promoted the coordination of O-based and N-based groups to Mn ions. The enhancement of the Mn-N and Mn-O bond content strengthened the oxidation-like activity of the carbon dots. The maximum reaction velocity and the Michaelis constant for 3, 3′, 5, 5′-tetramethylbenzidine were 19.8 × 10-5 mM s−1 and 0.159 mM, respectively. Finally, a platform for the dual-mode detection of nitrite was established based on the catalytic ability of the carbon dots for 3, 3′, 5, 5′-tetramethylbenzidine and the diazotization reaction of 3, 3′, 5, 5′-tetramethylbenzidine with nitrite. The response ranges of ratiometric colorimetric and fluorescence detection for nitrite were 0–200 μM and 0–100 μM, respectively. This work verified the feasibility of constructing carbon dots with oxidase-like activity and fluorescence property from carboxymethylcellulose, which can stimulate more efforts to explore the application of biomass-based carbon dots with oxidase-like activity in the field of nitrite detection.
{"title":"Cellulose-based carbon dots with fluorescence and oxidase-like activities: A tunable and sustainable substitute for natural enzymes to detect nitrite","authors":"Pengyu Hu , Yunchuan Dai , Tingting Chu , Jiliang Ma , Xing Wang , Zhiwei Wang , Pedram Fatehi , Yanzhu Guo","doi":"10.1016/j.biortech.2025.132511","DOIUrl":"10.1016/j.biortech.2025.132511","url":null,"abstract":"<div><div>The currently reported carbon dots with oxidase-like activity, as substitutes for natural enzymes, are generally prepared using organics with small molecules as carbon precursors. However, renewable cellulose as a feedstock is more conducive to the sustainable production of carbon dots. Herein, the Mn and N elements doped carbon dots with oxidase-like activity were prepared from carboxymethylcellulose, ethylenediamine, and MnCl<sub>2</sub>, and the effects of these three elements on the structure of the carbon dots were comprehensively studied and verified by advanced techniques. Moreover, the increase of ethylenediamine dosage and the prolongation of hydrothermal time promoted the coordination of O-based and <em>N</em>-based groups to Mn ions. The enhancement of the Mn-N and Mn-O bond content strengthened the oxidation-like activity of the carbon dots. The maximum reaction velocity and the Michaelis constant for 3, 3′, 5, 5′-tetramethylbenzidine were 19.8 × 10<sup>-5</sup> mM s<sup>−1</sup> and 0.159 mM, respectively. Finally, a platform for the dual-mode detection of nitrite was established based on the catalytic ability of the carbon dots for 3, 3′, 5, 5′-tetramethylbenzidine and the diazotization reaction of 3, 3′, 5, 5′-tetramethylbenzidine with nitrite. The response ranges of ratiometric colorimetric and fluorescence detection for nitrite were 0–200 μM and 0–100 μM, respectively. This work verified the feasibility of constructing carbon dots with oxidase-like activity and fluorescence property from carboxymethylcellulose, which can stimulate more efforts to explore the application of biomass-based carbon dots with oxidase-like activity in the field of nitrite detection.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132511"},"PeriodicalIF":9.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Poly (ethylene terephthalate) (PET) is a widely used plastic that leads to significant environmental pollution due to its durability. Enzymatic degradation of PET presents an eco-friendly disposal approach, with potential scalability for industrial applications. This review examines key crucial factors influencing PET enzymatic degradation, including the catalytic efficiency of PET hydrolase, production scalability of PET hydrolase, and recyclability of degraded PET. We outline major advancements in PET hydrolase development, including discovery techniques, functional enhancement strategies, and degradation optimization. Additionally, it assesses the preparation methodologies for PET hydrolase, covering bacterial expression systems, high-density fermentation technologies, and approaches for sustainable catalytic use. The review also discusses upcycling processes for PET hydrolysates, focusing on repolymerization into new plastics or bioconversion into valuable chemicals. Successful achievement of waste PET bio-disposal in industrial-scale n hinges on balancing degradation costs with revenue from upcycling products. Aim at this target, the review further points out the critical challenges, and proposes targeted solutions and expectations.
聚对苯二甲酸乙二醇酯（PET）是一种广泛使用的塑料，由于其耐久性而导致严重的环境污染。对 PET 进行酶降解是一种生态友好型处理方法，具有工业应用的潜在可扩展性。本综述探讨了影响 PET 酶降解的关键因素，包括 PET水解酶的催化效率、PET水解酶的生产可扩展性以及降解 PET 的可回收性。我们概述了 PET水解酶开发的主要进展，包括发现技术、功能增强策略和降解优化。此外，报告还评估了 PET水解酶的制备方法，包括细菌表达系统、高密度发酵技术和可持续催化使用方法。该综述还讨论了 PET 水解产物的升级再循环工艺，重点是将其重新聚合为新塑料或生物转化为有价值的化学品。能否成功实现工业规模的废弃 PET 生物处置，取决于降解成本与升级再循环产品收益之间的平衡。针对这一目标，综述进一步指出了关键挑战，并提出了有针对性的解决方案和期望。
{"title":"Current state and sustainable management of waste polyethylene terephthalate bio-disposal: enzymatic degradation to upcycling","authors":"Xiao-Qian Chen , De-Ming Rao , Xu-Yang Zhu , Xiao-Min Zhao , Qing-Song Huang , Jing Wu , Zheng-Fei Yan","doi":"10.1016/j.biortech.2025.132492","DOIUrl":"10.1016/j.biortech.2025.132492","url":null,"abstract":"<div><div>Poly (ethylene terephthalate) (PET) is a widely used plastic that leads to significant environmental pollution due to its durability. Enzymatic degradation of PET presents an eco-friendly disposal approach, with potential scalability for industrial applications. This review examines key crucial factors influencing PET enzymatic degradation, including the catalytic efficiency of PET hydrolase, production scalability of PET hydrolase, and recyclability of degraded PET. We outline major advancements in PET hydrolase development, including discovery techniques, functional enhancement strategies, and degradation optimization. Additionally, it assesses the preparation methodologies for PET hydrolase, covering bacterial expression systems, high-density fermentation technologies, and approaches for sustainable catalytic use. The review also discusses upcycling processes for PET hydrolysates, focusing on repolymerization into new plastics or bioconversion into valuable chemicals. Successful achievement of waste PET bio-disposal in industrial-scale n hinges on balancing degradation costs with revenue from upcycling products. Aim at this target, the review further points out the critical challenges, and proposes targeted solutions and expectations.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132492"},"PeriodicalIF":9.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-08DOI: 10.1016/j.biortech.2025.132506
Naroa Balsebre , Néstor Rojas , Felipe A. Díaz-Alvarado , Ana L. Prieto
We developed a novel integrated model for describing the A2MBR system, treating municipal wastewater for resource recovery. The model couples an anaerobic membrane bioreactor (AnMBR) with an algal photo membrane bioreactor (APMBR). The AnMBR details organic matter fractionation and its effect on SMP and EPS, while the APMBR incorporates alga-bacteria interaction, light, temperature, and membrane separation effects. While showing good agreement between simulated and measured values, results suggest that higher sludge retention time (SRT) drives COD removal, promoting biogas production in the AnMBR (2.5–3 days HRT and 60–90 days SRT not to exceed a 25 g MLSS/l). For the APMBR, shorter HRTs and longer SRTs promote microalgae growth, and longer HRTs enhance nutrient removal (maximum nutrient removal at 3 days HRT and 10 days SRT). Long-term operational data are needed to validate the proposed model. This modeling tool is valuable for modelers describing anaerobic/algal membrane processes for multi-purpose wastewater treatment.
{"title":"Integrated model of sequential anaerobic and algal membrane bioreactor (A2MBR) system for wastewater reuse and resource recovery","authors":"Naroa Balsebre , Néstor Rojas , Felipe A. Díaz-Alvarado , Ana L. Prieto","doi":"10.1016/j.biortech.2025.132506","DOIUrl":"10.1016/j.biortech.2025.132506","url":null,"abstract":"<div><div>We developed a novel integrated model for describing the A<sup>2</sup>MBR system, treating municipal wastewater for resource recovery. The model couples an anaerobic membrane bioreactor (AnMBR) with an algal photo membrane bioreactor (APMBR). The AnMBR details organic matter fractionation and its effect on SMP and EPS, while the APMBR incorporates alga-bacteria interaction, light, temperature, and membrane separation effects. While showing good agreement between simulated and measured values, results suggest that higher sludge retention time (SRT) drives COD removal, promoting biogas production in the AnMBR (2.5–3 days HRT and 60–90 days SRT not to exceed a 25 g MLSS/l). For the APMBR, shorter HRTs and longer SRTs promote microalgae growth, and longer HRTs enhance nutrient removal (maximum nutrient removal at 3 days HRT and 10 days SRT). Long-term operational data are needed to validate the proposed model. This modeling tool is valuable for modelers describing anaerobic/algal membrane processes for multi-purpose wastewater treatment.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132506"},"PeriodicalIF":9.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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