Pub Date : 2026-02-02DOI: 10.1016/j.biortech.2026.134140
Tianyi Zhang, Yanyan Li, Shen Cui, Feng Tian, Min Ye, Yu-You Li
{"title":"Insights into the future applications of Ca. Nitrosoglobus-driven acidic partial nitritation based on its unique characteristics: A critical review","authors":"Tianyi Zhang, Yanyan Li, Shen Cui, Feng Tian, Min Ye, Yu-You Li","doi":"10.1016/j.biortech.2026.134140","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.134140","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"87 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146109839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-02DOI: 10.1016/j.biortech.2026.134146
Xin Li, Shanshan Wu, Xueyu Tang
{"title":"Integrated omics-based analysis reveals the role of ferulic acid activating a co-production of 4-vinyl guaiacol and fumaric acid by Rhizopus oryzae","authors":"Xin Li, Shanshan Wu, Xueyu Tang","doi":"10.1016/j.biortech.2026.134146","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.134146","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"35 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146109840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Kraft pulping industry produces significant amounts of lignin-rich black liquor, a largely underutilized renewable resource. We report a scalable method to synthesize lignin-derived graphene quantum dots from raw eucalyptus kraft black liquor (EL-GQDs) through sequential acid oxidation and hydrothermal carbonization. Structural and surface analyses confirm the formation of graphitic nanostructures with oxygen- and nitrogen-rich functional groups, exhibiting strong visible fluorescence, good aqueous dispersibility, and high photostability, with uniform morphology and lattice fringes characteristic of crystalline GQDs. The bioimaging potential of EL-GQDs was tested using Nile tilapia testicular cells in vitro and in vivo, achieving efficient cell labeling with low toxicity. Labeled cells remained viable and localized within the gonads up to 30 days post-transplantation. These results demonstrate that EL-GQDs are biocompatible, photostable, and sustainable nanoprobes suitable for long-term cell tracking in aquatic reproductive research. Further studies on uptake pathways, cross-species application, and long-term biosafety are needed to advance this technology.
{"title":"Upcycling eucalyptus kraft lignin into fluorescent graphene quantum dots for reproductive cell imaging in aquaculture biotechnology.","authors":"Anna Octavera, Harits Atika Ariyanta, Khanesisya Luthfiyah Zahra, Diana Faoziah Hakim, Fajar Maulana, Novia Amalia Sholeha, Dian Hardiantho, Nurlatiha Khairun Nisa, Widya Fatriasari, Alimuddin","doi":"10.1016/j.biortech.2025.133769","DOIUrl":"10.1016/j.biortech.2025.133769","url":null,"abstract":"<p><p>The Kraft pulping industry produces significant amounts of lignin-rich black liquor, a largely underutilized renewable resource. We report a scalable method to synthesize lignin-derived graphene quantum dots from raw eucalyptus kraft black liquor (EL-GQDs) through sequential acid oxidation and hydrothermal carbonization. Structural and surface analyses confirm the formation of graphitic nanostructures with oxygen- and nitrogen-rich functional groups, exhibiting strong visible fluorescence, good aqueous dispersibility, and high photostability, with uniform morphology and lattice fringes characteristic of crystalline GQDs. The bioimaging potential of EL-GQDs was tested using Nile tilapia testicular cells in vitro and in vivo, achieving efficient cell labeling with low toxicity. Labeled cells remained viable and localized within the gonads up to 30 days post-transplantation. These results demonstrate that EL-GQDs are biocompatible, photostable, and sustainable nanoprobes suitable for long-term cell tracking in aquatic reproductive research. Further studies on uptake pathways, cross-species application, and long-term biosafety are needed to advance this technology.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133769"},"PeriodicalIF":9.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145686630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-01DOI: 10.1016/j.biortech.2026.134136
Sasha D. Hafner , Estelle Leca , Cecilia Sambusiti , Sergi Astals , Olivier Azam , Konrad Koch , Jing Liu , Henrik B. Møller , Mihaela Nistor , Mario Olaya-Rincon , Christine Peyrelasse , Alastair J. Ward , Bastien Zennaro , Florian Monlau
Thirty-one laboratories measured biochemical methane potential (BMP) of six substrates in a project aimed at improving BMP measurement quality. Laboratories used their established measurement procedures, with limited standardization. As in earlier studies, reproducibility was quantified, but here laboratories came from a wide geographic range, and the accuracy of BMP measurements was assessed by comparison with 6 experienced “reference” laboratories. Measurement reproducibility between the remaining “evaluation” laboratories was poor in the first test; relative reproducibility standard deviation was 21–33% for four substrates. Values improved to 9–19% in the second test following review of a detailed analysis of submitted data, troubleshooting meetings, and method modifications by some laboratories. Reference laboratories performed better, with reproducibility standard deviation of 5–12%. Repeatability within laboratories was also better for the reference group, with relative standard deviation of 2–4% vs. 3–7% for evaluation laboratories. Evaluation laboratory results showed a persistent negative bias of 4–16% compared to reference laboratories. Application of cellulose validation criteria improved reproducibility but not accuracy. Most of the extreme BMP values were associated with abnormal methane production curves, and a subjective visual evaluation proved valuable as a screening tool. The cause of measurement error was generally unknown, although data processing and volatile solids measurement errors caused some extreme results, and changes in inoculum source improved performance in two cases. There were no consistent differences among the diverse measurement methods and the sources of error probably vary among laboratories. Consistently accurate BMP measurement is possible for laboratories that follow established guidelines and develop experience.
{"title":"Reliable biochemical methane potential testing: insights and recommendations from global interlaboratory study","authors":"Sasha D. Hafner , Estelle Leca , Cecilia Sambusiti , Sergi Astals , Olivier Azam , Konrad Koch , Jing Liu , Henrik B. Møller , Mihaela Nistor , Mario Olaya-Rincon , Christine Peyrelasse , Alastair J. Ward , Bastien Zennaro , Florian Monlau","doi":"10.1016/j.biortech.2026.134136","DOIUrl":"10.1016/j.biortech.2026.134136","url":null,"abstract":"<div><div>Thirty-one laboratories measured biochemical methane potential (BMP) of six substrates in a project aimed at improving BMP measurement quality. Laboratories used their established measurement procedures, with limited standardization. As in earlier studies, reproducibility was quantified, but here laboratories came from a wide geographic range, and the accuracy of BMP measurements was assessed by comparison with 6 experienced “reference” laboratories. Measurement reproducibility between the remaining “evaluation” laboratories was poor in the first test; relative reproducibility standard deviation was 21–33% for four substrates. Values improved to 9–19% in the second test following review of a detailed analysis of submitted data, troubleshooting meetings, and method modifications by some laboratories. Reference laboratories performed better, with reproducibility standard deviation of 5–12%. Repeatability within laboratories was also better for the reference group, with relative standard deviation of 2–4% vs. 3–7% for evaluation laboratories. Evaluation laboratory results showed a persistent negative bias of 4–16% compared to reference laboratories. Application of cellulose validation criteria improved reproducibility but not accuracy. Most of the extreme BMP values were associated with abnormal methane production curves, and a subjective visual evaluation proved valuable as a screening tool. The cause of measurement error was generally unknown, although data processing and volatile solids measurement errors caused some extreme results, and changes in inoculum source improved performance in two cases. There were no consistent differences among the diverse measurement methods and the sources of error probably vary among laboratories. Consistently accurate BMP measurement is possible for laboratories that follow established guidelines and develop experience.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"446 ","pages":"Article 134136"},"PeriodicalIF":9.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146098426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-01Epub Date: 2025-12-18DOI: 10.1016/j.biortech.2025.133791
Zhan-Ling Wang, Bin Yang, Jing Cao, De-Peng Wang, Xu-Xiang Zhang, Mei Li
{"title":"Erratum to \"Molecular insights into anammox inhibition induced by environmentally relevant sulfamonomethoxine and functional recovery via voltage stimulation\" [Bioresour. Technol. 436 (2025) 133018].","authors":"Zhan-Ling Wang, Bin Yang, Jing Cao, De-Peng Wang, Xu-Xiang Zhang, Mei Li","doi":"10.1016/j.biortech.2025.133791","DOIUrl":"10.1016/j.biortech.2025.133791","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133791"},"PeriodicalIF":9.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145779833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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