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Substrate preference triggers metabolic patterns of indigenous microbiome during initial composting stages
IF 11.4 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2025-01-04 DOI: 10.1016/j.biortech.2024.132034
Yi Ren, Chen Liu, Jiayu Luo, Xuhui Deng, Daoyue Zheng, Jiahui Shao, Zhihui Xu, Nan Zhang, Wu Xiong, Hongjun Liu, Rong Li, Youzhi Miao, Ruifu Zhang, Qirong Shen, Weibing Xun
Composting organic waste is a sustainable recycling method in agricultural systems, yet the microbial preferences for different substrates and their influence on composting efficiency remain underexplored. Here, 210 datasets of published 16S ribosomal DNA amplicon sequences from straw and manure composts worldwide were analyzed, and a database of 278 bacterial isolates was compiled. Substrate-driven microbiome variations were most prominent during the initial composting stages. Indigenous synthetic communities exhibit substrate-specific adaptations, increasing compost temperatures by 2 %-10 %, microbial abundance by 44 %–233 %, and microbial activity by 26 %-60 %. Key dissolved substrates, such as choline and succinic acid in straw compost, and phloretin and uric acid in manure compost, drive these microbial preferences. These findings highlight how substrate-specific microbiomes can be engineered to enhance microbial activity, accelerate temperature rise, and extend the thermophilic phase, providing a targeted framework to improve composting efficiency and tailor strategies to different organic waste types.
{"title":"Substrate preference triggers metabolic patterns of indigenous microbiome during initial composting stages","authors":"Yi Ren, Chen Liu, Jiayu Luo, Xuhui Deng, Daoyue Zheng, Jiahui Shao, Zhihui Xu, Nan Zhang, Wu Xiong, Hongjun Liu, Rong Li, Youzhi Miao, Ruifu Zhang, Qirong Shen, Weibing Xun","doi":"10.1016/j.biortech.2024.132034","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.132034","url":null,"abstract":"Composting organic waste is a sustainable recycling method in agricultural systems, yet the microbial preferences for different substrates and their influence on composting efficiency remain underexplored. Here, 210 datasets of published 16S ribosomal DNA amplicon sequences from straw and manure composts worldwide were analyzed, and a database of 278 bacterial isolates was compiled. Substrate-driven microbiome variations were most prominent during the initial composting stages. Indigenous synthetic communities exhibit substrate-specific adaptations, increasing compost temperatures by 2 %-10 %, microbial abundance by 44 %–233 %, and microbial activity by 26 %-60 %. Key dissolved substrates, such as choline and succinic acid in straw compost, and phloretin and uric acid in manure compost, drive these microbial preferences. These findings highlight how substrate-specific microbiomes can be engineered to enhance microbial activity, accelerate temperature rise, and extend the thermophilic phase, providing a targeted framework to improve composting efficiency and tailor strategies to different organic waste types.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"74 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142936028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibiotic resistance reduction mechanisms during thermophilic anaerobic digestion of microalgae-bacteria aggregates.
IF 9.7 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2025-01-03 DOI: 10.1016/j.biortech.2025.132037
Julián O Ovis-Sánchez, Miguel Vital-Jácome, Germán Buitrón, Pabel Cervantes-Avilés, Julián Carrillo-Reyes

Microalgae-bacteria-based systems are an emerging and promising approach for wastewater treatment plants (WWTP), having nutrient and antibiotic resistance removal comparable to conventional technologies. Still, antibiotic-resistance genes and bacteria (ARG and ARB) can proliferate in microalga-bacteria aggregates (MABA), a concern to control. Different temperature regimes of MABA continuous anaerobic digestion (AD), thermophilic (55 °C), and mesophilic (35 °C) were evaluated in this study as a strategy to eliminate ARB and ARGs. Plate counting techniques and metagenomic-based analysis revealed that thermophilic temperature had a better performance, achieving ARB log reductions of 1.1 to 1.7 for various antibiotics and significantly reduced ARG abundance up to 19.5 ± 0.8 ppm. The microbiome selection, the mobilome restriction, and directed functionality to thermal stress resistance were the main mechanisms responsible for resistome reduction at thermophilic conditions. Thermophilic AD effectively manages antibiotic resistance in microalgae-bacteria aggregates, which has important implications for wastewater treatment and reduces environmental risks.

{"title":"Antibiotic resistance reduction mechanisms during thermophilic anaerobic digestion of microalgae-bacteria aggregates.","authors":"Julián O Ovis-Sánchez, Miguel Vital-Jácome, Germán Buitrón, Pabel Cervantes-Avilés, Julián Carrillo-Reyes","doi":"10.1016/j.biortech.2025.132037","DOIUrl":"10.1016/j.biortech.2025.132037","url":null,"abstract":"<p><p>Microalgae-bacteria-based systems are an emerging and promising approach for wastewater treatment plants (WWTP), having nutrient and antibiotic resistance removal comparable to conventional technologies. Still, antibiotic-resistance genes and bacteria (ARG and ARB) can proliferate in microalga-bacteria aggregates (MABA), a concern to control. Different temperature regimes of MABA continuous anaerobic digestion (AD), thermophilic (55 °C), and mesophilic (35 °C) were evaluated in this study as a strategy to eliminate ARB and ARGs. Plate counting techniques and metagenomic-based analysis revealed that thermophilic temperature had a better performance, achieving ARB log reductions of 1.1 to 1.7 for various antibiotics and significantly reduced ARG abundance up to 19.5 ± 0.8 ppm. The microbiome selection, the mobilome restriction, and directed functionality to thermal stress resistance were the main mechanisms responsible for resistome reduction at thermophilic conditions. Thermophilic AD effectively manages antibiotic resistance in microalgae-bacteria aggregates, which has important implications for wastewater treatment and reduces environmental risks.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132037"},"PeriodicalIF":9.7,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Engineering an Mn(II)-oxidizing Pseudomonas whole-cell catalyst chassis to efficiently biosynthesize 2,5-furandicarboxylic acid from hydroxymethylfurfural.
IF 9.7 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2025-01-03 DOI: 10.1016/j.biortech.2025.132036
Yongxuan Liu, Zhicheng Zhou, Jingjing Xu, Shiwei Li, Yu Xiao, Xun Yu, Tan Wang, Jie Zeng, Lin Li

2,5-Furandicarboxylic acid (FDCA) is a high-value chemical extensively used in the production of bio-based polymers, but bioconversion of furan derivatives like 5-hydroxymethylfurfural (HMF) into FDCA remains challenging owing to substrate cytotoxicity. Here, we engineered an Mn(II)-oxidizing Pseudomonas sp. MB04B for efficient FDCA biosynthesis from HMF. We deleted 4.6 % of the MB04B genome to generate the engineered MB04C-6 chassis, then introduced two exogenous gene cassettes, PMP00-hmfH and PJ23119-hmfH'. Using the resulting MB04C-6/pHMF as a whole-cell catalyst, optimizing the reaction system, and incorporating CaCO3 increased the FDCA yield by approximately 63.4-fold compared to MB04C-6. We also enhanced the CRISPR-associated transposases system for single-step chromosomal integration of exogenous genes. The optimal chassis strain MB04S-HMF8, rapidly produced 97 mmol/L FDCA from 100 mmol/L HMF in 12 h, with an FDCA production rate of 1.26 g L-1h-1, showcasing its potential as a robust, cost-effective, and environmentally sustainable whole-cell biocatalyst for industrial-scale FDCA production.

{"title":"Engineering an Mn(II)-oxidizing Pseudomonas whole-cell catalyst chassis to efficiently biosynthesize 2,5-furandicarboxylic acid from hydroxymethylfurfural.","authors":"Yongxuan Liu, Zhicheng Zhou, Jingjing Xu, Shiwei Li, Yu Xiao, Xun Yu, Tan Wang, Jie Zeng, Lin Li","doi":"10.1016/j.biortech.2025.132036","DOIUrl":"10.1016/j.biortech.2025.132036","url":null,"abstract":"<p><p>2,5-Furandicarboxylic acid (FDCA) is a high-value chemical extensively used in the production of bio-based polymers, but bioconversion of furan derivatives like 5-hydroxymethylfurfural (HMF) into FDCA remains challenging owing to substrate cytotoxicity. Here, we engineered an Mn(II)-oxidizing Pseudomonas sp. MB04B for efficient FDCA biosynthesis from HMF. We deleted 4.6 % of the MB04B genome to generate the engineered MB04C-6 chassis, then introduced two exogenous gene cassettes, P<sub>MP00</sub>-hmfH and P<sub>J23119</sub>-hmfH'. Using the resulting MB04C-6/pHMF as a whole-cell catalyst, optimizing the reaction system, and incorporating CaCO<sub>3</sub> increased the FDCA yield by approximately 63.4-fold compared to MB04C-6. We also enhanced the CRISPR-associated transposases system for single-step chromosomal integration of exogenous genes. The optimal chassis strain MB04S-HMF8, rapidly produced 97 mmol/L FDCA from 100 mmol/L HMF in 12 h, with an FDCA production rate of 1.26 g L<sup>-1</sup>h<sup>-1</sup>, showcasing its potential as a robust, cost-effective, and environmentally sustainable whole-cell biocatalyst for industrial-scale FDCA production.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132036"},"PeriodicalIF":9.7,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polyphenolic compounds mitigate the oxidative damage of anammox sludge under long-term light irradiation.
IF 9.7 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2025-01-03 DOI: 10.1016/j.biortech.2025.132038
Zhi-Qi Ren, Rong-Rong Chang, Hao Wang, Gui-Feng Li, Bao-Cheng Huang, Ren-Cun Jin

Continuous high-intensity light exposure can inhibit anaerobic ammonium oxidation (anammox) bacteria activity, though the specific impacts on anammox reactor performance remain unclear. This study investigates the effects of long-term light stress on anammox sludge reactors and explores the use of tea polyphenols as an engineering interventions to mitigate photo oxidation damage. The results showed that the nitrogen removal efficiency (NRE) of the reactor rapidly deteriorated to 41.4 % under 10,000 lx light conditions. However, reactors supplemented with 1 mg·L-1 and 5 mg·L-1 tea polyphenols sustained NREs of 75.2 % and 82.5 %, respectively. The addition of tea polyphenols alleviated oxidative stress by scavenging reactive oxygen species such as ·OH and H2O2, and by enhancing the activities of antioxidant enzymes including total superoxide dismutase and glutathione peroxidase. Candidatus Kuenenia was negatively impacted by light, while unclassified_f__Brocadiaceae thrived under light stress. These findings provide insights for the development of stable nitrogen removal systems under light exposure.

{"title":"Polyphenolic compounds mitigate the oxidative damage of anammox sludge under long-term light irradiation.","authors":"Zhi-Qi Ren, Rong-Rong Chang, Hao Wang, Gui-Feng Li, Bao-Cheng Huang, Ren-Cun Jin","doi":"10.1016/j.biortech.2025.132038","DOIUrl":"10.1016/j.biortech.2025.132038","url":null,"abstract":"<p><p>Continuous high-intensity light exposure can inhibit anaerobic ammonium oxidation (anammox) bacteria activity, though the specific impacts on anammox reactor performance remain unclear. This study investigates the effects of long-term light stress on anammox sludge reactors and explores the use of tea polyphenols as an engineering interventions to mitigate photo oxidation damage. The results showed that the nitrogen removal efficiency (NRE) of the reactor rapidly deteriorated to 41.4 % under 10,000 lx light conditions. However, reactors supplemented with 1 mg·L<sup>-1</sup> and 5 mg·L<sup>-1</sup> tea polyphenols sustained NREs of 75.2 % and 82.5 %, respectively. The addition of tea polyphenols alleviated oxidative stress by scavenging reactive oxygen species such as ·OH and H<sub>2</sub>O<sub>2</sub>, and by enhancing the activities of antioxidant enzymes including total superoxide dismutase and glutathione peroxidase. Candidatus Kuenenia was negatively impacted by light, while unclassified_f__Brocadiaceae thrived under light stress. These findings provide insights for the development of stable nitrogen removal systems under light exposure.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132038"},"PeriodicalIF":9.7,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced butanol tolerance and production from puerariae slag hydrolysate by Clostridium beijerinckii through metabolic engineering and process regulation strategies.
IF 9.7 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2025-01-02 DOI: 10.1016/j.biortech.2025.132035
Zhiyou Zhou, Huanhuan Ding, Chaoyue Shi, Shuaiyin Peng, Biao Zhu, Xuejiao An, Hanguang Li

Butanol is a more desirable second-generation biomass energy source. Acetone-butanol-ethanol (ABE) fermentation using Clostridium spp. is a promising method for butanol production. However, the toxicity of butanol to the producing strains leading to its low yield and the high cost of feedstock are the main obstacles limiting the ABE fermentation industry. In this study, to enhance the butanol tolerance and production in Clostridium beijerinckii D9, the strategies of metabolic engineering and process regulation were employed. With this effort, a recombinant strain D9/pykA was successfully developed. Furthermore, the effect of exogenous fermentation waste streams and their two-stage addition strategy on ABE fermentation was also investigated. Under the optimal condition, the highest butanol and total solvent production of 11.20 ± 0.58 g/L and 13.65 ± 0.51 g/L was achieved in C. beijerinckii D9/pykA, representing increases of 40.70 % and 37.05 %, respectively, compared to the original strain D9. Additionally, the results of the physiological mechanism revealed that the two-stage fermentation waste stream addition improved NADH synthesis and upregulated key genes involved in butanol biosynthesis, and thus enhancing the production. These insights could provide a foundation for further optimization of ABE fermentation processes and offer promising avenues for improving other similar research.

{"title":"Enhanced butanol tolerance and production from puerariae slag hydrolysate by Clostridium beijerinckii through metabolic engineering and process regulation strategies.","authors":"Zhiyou Zhou, Huanhuan Ding, Chaoyue Shi, Shuaiyin Peng, Biao Zhu, Xuejiao An, Hanguang Li","doi":"10.1016/j.biortech.2025.132035","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132035","url":null,"abstract":"<p><p>Butanol is a more desirable second-generation biomass energy source. Acetone-butanol-ethanol (ABE) fermentation using Clostridium spp. is a promising method for butanol production. However, the toxicity of butanol to the producing strains leading to its low yield and the high cost of feedstock are the main obstacles limiting the ABE fermentation industry. In this study, to enhance the butanol tolerance and production in Clostridium beijerinckii D9, the strategies of metabolic engineering and process regulation were employed. With this effort, a recombinant strain D9/pykA was successfully developed. Furthermore, the effect of exogenous fermentation waste streams and their two-stage addition strategy on ABE fermentation was also investigated. Under the optimal condition, the highest butanol and total solvent production of 11.20 ± 0.58 g/L and 13.65 ± 0.51 g/L was achieved in C. beijerinckii D9/pykA, representing increases of 40.70 % and 37.05 %, respectively, compared to the original strain D9. Additionally, the results of the physiological mechanism revealed that the two-stage fermentation waste stream addition improved NADH synthesis and upregulated key genes involved in butanol biosynthesis, and thus enhancing the production. These insights could provide a foundation for further optimization of ABE fermentation processes and offer promising avenues for improving other similar research.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132035"},"PeriodicalIF":9.7,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High-throughput screening of acetogenic strains for growth and metabolite profiles on readily available biomass.
IF 9.7 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2025-01-02 DOI: 10.1016/j.biortech.2024.132026
Natálie Palucha, Koen Quataert, Elodie Vlaeminck, Eliot Schröder, Karel De Winter, Wim Soetaert

Abundant biomass, including industrial waste streams and second-generation (2G) and third-generation (3G) feedstocks, offers significant potential for sustainable bioconversion, nevertheless challenges such as fermentation inhibitors, CO2 losses and substrate selectivity of traditional microbial hosts hinder process efficiency. In this study, we address these challenges by exploring acetogenic bacteria as alternative microbial hosts. Using a newly established high-throughput method, acetogens were evaluated for their capacity to hydrolyse and metabolize variety of substrates derived from 2G and 3G feedstocks and industrial waste streams. Our findings demonstrate metabolic versatility of acetogens in converting biomass-derived substrates into a wide array of products while also exhibiting resilience to common fermentation inhibitors. These unique capabilities position acetogens as promising alternatives that could potentially outperform conventional production hosts in achieving 100% biomass valorization while underscoring the need for further research into critical areas, such as the utilization of mixed substrates under industrially relevant conditions.

{"title":"High-throughput screening of acetogenic strains for growth and metabolite profiles on readily available biomass.","authors":"Natálie Palucha, Koen Quataert, Elodie Vlaeminck, Eliot Schröder, Karel De Winter, Wim Soetaert","doi":"10.1016/j.biortech.2024.132026","DOIUrl":"10.1016/j.biortech.2024.132026","url":null,"abstract":"<p><p>Abundant biomass, including industrial waste streams and second-generation (2G) and third-generation (3G) feedstocks, offers significant potential for sustainable bioconversion, nevertheless challenges such as fermentation inhibitors, CO<sub>2</sub> losses and substrate selectivity of traditional microbial hosts hinder process efficiency. In this study, we address these challenges by exploring acetogenic bacteria as alternative microbial hosts. Using a newly established high-throughput method, acetogens were evaluated for their capacity to hydrolyse and metabolize variety of substrates derived from 2G and 3G feedstocks and industrial waste streams. Our findings demonstrate metabolic versatility of acetogens in converting biomass-derived substrates into a wide array of products while also exhibiting resilience to common fermentation inhibitors. These unique capabilities position acetogens as promising alternatives that could potentially outperform conventional production hosts in achieving 100% biomass valorization while underscoring the need for further research into critical areas, such as the utilization of mixed substrates under industrially relevant conditions.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132026"},"PeriodicalIF":9.7,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lignin nano/micro-particles from agricultural biomasses: Developing direct precipitation for integrated biorefinery.
IF 9.7 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2024-12-31 DOI: 10.1016/j.biortech.2024.132025
Tuyen B Ly, Dat D B Nguyen, Anh M H Trinh, Nhi T T Tran, Thi H M Truong, Kien A Le, Ha V Le, Phung K Le

The state-of-the-art, simple and scalable methods for lignin micro-/nano-particles recovery from agricultural biomasses were evaluated in this review. Being non-wood biomasses, these materials can be easily fibrillated, supporting the usage of mild soda or organic solvent pretreatment. Different approaches in particle recovery were compared to conclude that the bottom-up approach facilitates smaller particles towards the nano-size range whereas mechanical treatment can act as a supporting method to increase uniformity and reduce particle sizes after bottom-up precipitation. By combining with the pretreatment steps, direct one-pot lignin micro-/nano-particle recovery can be achieved using the lignin-containing black liquor or organosolv liquor. These lignin micro-/nano-particles can then be applied as high-value functional products in cosmetics, pharmaceuticals, environmental remediation, and energy sectors. The systematic evaluation of lignin micro-/nano-particles recovery from agricultural biomasses in this review can support the full utilization of these natural resources to aim towards a circular agriculture.

本综述评估了从农业生物质中回收木质素微/纳米颗粒的最先进、简单和可扩展的方法。作为非木材生物质,这些材料很容易纤维化,因此可以使用温和的苏打水或有机溶剂进行预处理。对颗粒回收的不同方法进行了比较,得出的结论是,自下而上的方法可使颗粒更小,达到纳米尺寸范围,而机械处理可作为一种辅助方法,在自下而上沉淀后提高均匀性并减小颗粒尺寸。通过与预处理步骤相结合,可利用含木质素的黑液或有机溶液实现直接的一锅式木质素微/纳米颗粒回收。这些木质素微/纳米颗粒可作为高价值功能性产品应用于化妆品、医药、环境修复和能源领域。本综述对从农业生物质中回收木质素微粒/纳米颗粒进行了系统评估,有助于充分利用这些自然资源,实现循环农业。
{"title":"Lignin nano/micro-particles from agricultural biomasses: Developing direct precipitation for integrated biorefinery.","authors":"Tuyen B Ly, Dat D B Nguyen, Anh M H Trinh, Nhi T T Tran, Thi H M Truong, Kien A Le, Ha V Le, Phung K Le","doi":"10.1016/j.biortech.2024.132025","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.132025","url":null,"abstract":"<p><p>The state-of-the-art, simple and scalable methods for lignin micro-/nano-particles recovery from agricultural biomasses were evaluated in this review. Being non-wood biomasses, these materials can be easily fibrillated, supporting the usage of mild soda or organic solvent pretreatment. Different approaches in particle recovery were compared to conclude that the bottom-up approach facilitates smaller particles towards the nano-size range whereas mechanical treatment can act as a supporting method to increase uniformity and reduce particle sizes after bottom-up precipitation. By combining with the pretreatment steps, direct one-pot lignin micro-/nano-particle recovery can be achieved using the lignin-containing black liquor or organosolv liquor. These lignin micro-/nano-particles can then be applied as high-value functional products in cosmetics, pharmaceuticals, environmental remediation, and energy sectors. The systematic evaluation of lignin micro-/nano-particles recovery from agricultural biomasses in this review can support the full utilization of these natural resources to aim towards a circular agriculture.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132025"},"PeriodicalIF":9.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Roles of waste iron scraps in anammox system treating sulfide-containing wastewater: Alleviating sulfide inhibition, promoting novel anammox bacteria enrichment, and enhancing nitrogen removal capacity
IF 11.4 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2024-12-31 DOI: 10.1016/j.biortech.2024.132033
Yu Feng, Xingyu Zhang, Caiwei Zhang, Haozhe Xu, Xiaoming Ji, Jianfang Wang, Peng Wu, Feiyue Qian, Chongjun Chen, Yaoliang Shen, Wenru Liu
In this study, waste iron scraps (WIS) were exerted to alleviate sulfide inhibition on anammox bacteria and promote anammox nitrogen removal from sulfide-containing wastewater.Short-term batch experiments showed that WIS-addition led to the anammox bacteria activity increasing by 124.8 % at an initial sulfide concentration of 40 mgS/L. During the long-term experiments, the nitrogen removal rate (NRR) reached to 8.76 kg/(m3·d) in the WIS-added reactor, while the maximum NRR was only 3.77 ± 0.31 kg/(m3·d) in the non-WIS reactor. In contrast to anammox bacteria development in the non-WIS reactor, the relative abundance of Candidatus Kuenenia (1.4–3.7 %) declined significantly in the WIS-added reactor, but novel potential anammox bacteria Brocadiaceae_unclassified (60.1 %-78.6 %) were highly enriched. Overall, the experimental evidence suggested that WIS-addition not only mitigated the sulfide inhibition on anammox bacteria, but also promoted novel anammox bacteria proliferation. The findings of this work provide a promising solution for wide engineering applications of anammox treating sulfide-containing wastewater.
{"title":"Roles of waste iron scraps in anammox system treating sulfide-containing wastewater: Alleviating sulfide inhibition, promoting novel anammox bacteria enrichment, and enhancing nitrogen removal capacity","authors":"Yu Feng, Xingyu Zhang, Caiwei Zhang, Haozhe Xu, Xiaoming Ji, Jianfang Wang, Peng Wu, Feiyue Qian, Chongjun Chen, Yaoliang Shen, Wenru Liu","doi":"10.1016/j.biortech.2024.132033","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.132033","url":null,"abstract":"In this study, waste iron scraps (WIS) were exerted to alleviate sulfide inhibition on anammox bacteria and promote anammox nitrogen removal from sulfide-containing wastewater.<ce:hsp sp=\"0.25\"></ce:hsp>Short-term batch experiments showed that WIS-addition led to the anammox bacteria activity increasing by 124.8 % at an initial sulfide concentration of 40 mgS/L. During the long-term experiments, the nitrogen removal rate (NRR) reached to 8.76 kg/(m<ce:sup loc=\"post\">3</ce:sup>·d) in the WIS-added reactor, while the maximum NRR was only 3.77 ± 0.31 kg/(m<ce:sup loc=\"post\">3</ce:sup>·d) in the non-WIS reactor. In contrast to anammox bacteria development in the non-WIS reactor, the relative abundance of <ce:italic>Candidatus Kuenenia</ce:italic> (1.4–3.7 %) declined significantly in the WIS-added reactor, but novel potential anammox bacteria <ce:italic>Brocadiaceae_unclassified</ce:italic> (60.1 %-78.6 %) were highly enriched. Overall, the experimental evidence suggested that WIS-addition not only mitigated the sulfide inhibition on anammox bacteria, but also promoted novel anammox bacteria proliferation. The findings of this work provide a promising solution for wide engineering applications of anammox treating sulfide-containing wastewater.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"16 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142911930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Review on synthesis of lactic acid and lactates from biomass derived carbohydrates via chemocatalysis routes
IF 11.4 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2024-12-31 DOI: 10.1016/j.biortech.2024.132031
Ruite Lai, Fei Qu, Meiting Ju, Chao Xie, Hengli Qian, Tianliang Xia, Chengxu Wang, Guanjie Yu, Yao Tang, Xinyu Bai, Qidong Hou
The utilization of renewable lignocellulosic biomass resources is a promising solution to deal with the deficit of fossil resources and the associated environmental concerns. Among diverse biomass-derived products, lactic acid (LA) stands out as one of the most successful commodities and also a platform to connect raw biomass feedstocks with value-added chemicals and degradable polymers. Herein, we critically review the recent advances in the design and development of base, acid, and multifunctional catalytic systems for the conversion of different carbohydrates to LA and alkyl lactates via chemical routes. In addition to critically evaluating the advantages and disadvantages of different catalytic systems, we provide deep insights into the reaction mechanisms, including the reaction pathways of different feedstocks, the catalytic roles of different kinds of active sites, and the structure–activity relationship. We conclude with our perspective on the key challenges and future opportunities.
{"title":"Review on synthesis of lactic acid and lactates from biomass derived carbohydrates via chemocatalysis routes","authors":"Ruite Lai, Fei Qu, Meiting Ju, Chao Xie, Hengli Qian, Tianliang Xia, Chengxu Wang, Guanjie Yu, Yao Tang, Xinyu Bai, Qidong Hou","doi":"10.1016/j.biortech.2024.132031","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.132031","url":null,"abstract":"The utilization of renewable lignocellulosic biomass resources is a promising solution to deal with the deficit of fossil resources and the associated environmental concerns. Among diverse biomass-derived products<ce:bold>,</ce:bold> lactic acid (LA) stands out as one of the most successful commodities and also a platform to connect raw biomass feedstocks with value-added chemicals and degradable polymers. Herein, we critically review the recent advances in the design and development of base, acid, and multifunctional catalytic systems for the conversion of different carbohydrates to LA and alkyl lactates via chemical routes. In addition to critically evaluating the advantages and disadvantages of different catalytic systems, we provide deep insights into the reaction mechanisms, including the reaction pathways of different feedstocks, the catalytic roles of different kinds of active sites, and the structure–activity relationship. We conclude with our perspective on the key challenges and future opportunities.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"34 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142911931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced cyanophycin accumulation in diazotrophic cyanobacterium through random mutagenesis and tailored selection under varying phosphorus availability.
IF 9.7 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2024-12-31 DOI: 10.1016/j.biortech.2024.132018
Marta Carletti, Eleonora Sforza, Albert Batushansky, Sammy Boussiba, Alberto Bertucco, Inna Khozin-Goldberg, Boris Zorin

This study explored a sustainable alternative to the Haber-Bosch process by enhancing the production of the nitrogen-rich polymer cyanophycin (CGP) in the diazotrophic cyanobacterium Nostoc sp. PCC 7120. Applying UV-mutagenesis followed by canavanine selection, we isolate an initial mutant with enhanced CGP accumulation. Subsequently, a secondary selection under phosphorus-limited conditions was employed to decrease cellular ploidy, yielding stable mutants. Among these, strain 44 exhibited an improved CGP accumulation, achieving up to 34 % of cellular dry weight in batch cultures. Under continuous phosphorus-limited cultivation, this mutant demonstrated a CGP productivity of 63 mg L-1 day-1, approximately a fourfold improvement over the wild type. Genomic analysis of the mutants revealed mutations unrelated to known CGP biosynthetic pathways, suggesting that the observed enhancement in CGP may arise from complex, synergistic effects of multiple genetic changes. This integrative approach-combining mutagenesis, screening, and cultivation techniques-successfully increased CGP accumulation from atmospheric nitrogen over threefold compared to the wild-type.

本研究通过提高重氮蓝藻 Nostoc sp. PCC7120 中富氮聚合物蓝藻霉素(CGP)的产量,探索了哈伯-博什工艺的可持续替代方法。通过紫外线诱变和卡纳瓦宁选择,我们分离出了一个具有增强 CGP 积累能力的初始突变体。随后,我们在磷限制条件下进行了二次选择,以降低细胞倍性,从而获得了稳定的突变体。在这些突变体中,菌株 44 的 CGP 积累能力有所提高,在批量培养中可达到细胞干重的 34%。在连续磷限制培养条件下,该突变体的 CGP 生产率达到 63 mg L-1 day-1,比野生型提高了约四倍。对突变体的基因组分析表明,突变与已知的 CGP 生物合成途径无关,这表明观察到的 CGP 增强可能来自多种基因变化的复杂协同效应。这种综合方法结合了诱变、筛选和培养技术,成功地将来自大气氮的 CGP 积累提高到野生型的三倍以上。
{"title":"Enhanced cyanophycin accumulation in diazotrophic cyanobacterium through random mutagenesis and tailored selection under varying phosphorus availability.","authors":"Marta Carletti, Eleonora Sforza, Albert Batushansky, Sammy Boussiba, Alberto Bertucco, Inna Khozin-Goldberg, Boris Zorin","doi":"10.1016/j.biortech.2024.132018","DOIUrl":"10.1016/j.biortech.2024.132018","url":null,"abstract":"<p><p>This study explored a sustainable alternative to the Haber-Bosch process by enhancing the production of the nitrogen-rich polymer cyanophycin (CGP) in the diazotrophic cyanobacterium Nostoc sp. PCC 7120. Applying UV-mutagenesis followed by canavanine selection, we isolate an initial mutant with enhanced CGP accumulation. Subsequently, a secondary selection under phosphorus-limited conditions was employed to decrease cellular ploidy, yielding stable mutants. Among these, strain 44 exhibited an improved CGP accumulation, achieving up to 34 % of cellular dry weight in batch cultures. Under continuous phosphorus-limited cultivation, this mutant demonstrated a CGP productivity of 63 mg L<sup>-1</sup> day<sup>-1</sup>, approximately a fourfold improvement over the wild type. Genomic analysis of the mutants revealed mutations unrelated to known CGP biosynthetic pathways, suggesting that the observed enhancement in CGP may arise from complex, synergistic effects of multiple genetic changes. This integrative approach-combining mutagenesis, screening, and cultivation techniques-successfully increased CGP accumulation from atmospheric nitrogen over threefold compared to the wild-type.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132018"},"PeriodicalIF":9.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Bioresource Technology
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