Pub Date : 2024-11-10DOI: 10.1016/j.biortech.2024.131799
Yuliang Zhu , Dong Li , Ben Ma , Huiping Zeng , Jie Zhang
The stability of anaerobic ammonium oxidation (anammox) performance is inseparably linked to the dynamic equilibrium of microbial interactions. However, understanding of the key microbes within anammox systems remains limited. Through the analysis of 186 16S rRNA datasets combined with various ecological analysis methods, this study identified key microbes in the anammox process. Interactions between Candidatus_Kuenenia and other key microbes are the most significant with greater tolerance to differing water quality, while Candidatus_Jettenia have higher habitat specificity. Under adverse conditions, anammox bacteria can reduce the impact of unfavorable environments by enhancing interactions with certain microbes. This study comprehensively reviews the main functions of key microbes in the anammox system and their interactions, and summarizes several common interaction mechanisms, providing new insights for understanding the startup and stable operation of the anammox process.
{"title":"Deciphering key microbes and their interactions within anaerobic ammonia oxidation systems","authors":"Yuliang Zhu , Dong Li , Ben Ma , Huiping Zeng , Jie Zhang","doi":"10.1016/j.biortech.2024.131799","DOIUrl":"10.1016/j.biortech.2024.131799","url":null,"abstract":"<div><div>The stability of anaerobic ammonium oxidation (anammox) performance is inseparably linked to the dynamic equilibrium of microbial interactions. However, understanding of the key microbes within anammox systems remains limited. Through the analysis of 186 16S rRNA datasets combined with various ecological analysis methods, this study identified key microbes in the anammox process. Interactions between <em>Candidatus_Kuenenia</em> and other key microbes are the most significant with greater tolerance to differing water quality, while <em>Candidatus_Jettenia</em> have higher habitat specificity. Under adverse conditions, anammox bacteria can reduce the impact of unfavorable environments by enhancing interactions with certain microbes. This study comprehensively reviews the main functions of key microbes in the anammox system and their interactions, and summarizes several common interaction mechanisms, providing new insights for understanding the startup and stable operation of the anammox process.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131799"},"PeriodicalIF":9.7,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-10DOI: 10.1016/j.biortech.2024.131789
Shiteng Tan , Ruikun Wang , Jialiang Dong , Kai Zhang , Zhenghui Zhao , Qianqian Yin , Jingwei Liu , Weijie Yang , Jun Cheng
Nitrogen-doped carbon materials are deemed promising cathode catalysts for microbial fuel cells (MFCs). The challenge lies in reducing costs and enhancing the proportion of electrocatalytically active nitrogenous functional groups. This study proposes a hydrothermal-mediated in-situ doping method to produce nitrogen-doped biochar from aquatic plants. The nitrogen atoms are anchored in the carbon structure during hydrothermal treatment. Subsequent pyrolysis converts the hydrochar into a catalyst with highly catalytically active aromatic ring structure (HC-N+PY). The as-prepared HC-N+PY electrocatalyst demonstrates superior oxygen reduction reaction activity with half-wave potentials of 0.82 V. The MFC with HC-N+PY exhibits excellent performance, with a peak power density of 1444 mW/m2. Theoretical calculations demonstrate that the synergistic effect of graphitic nitrogen and C–O groups at defect sites enhances O2 adsorption and protonation. This work highlights the potential of utilizing nitrogen-doped biochar derived from aquatic plants as an effective catalyst for enhancing the performance of microbial fuel cells.
{"title":"Hydrothermal-mediated in-situ nitrogen doping to prepare biochar for enhancing oxygen reduction reactions in microbial fuel cells","authors":"Shiteng Tan , Ruikun Wang , Jialiang Dong , Kai Zhang , Zhenghui Zhao , Qianqian Yin , Jingwei Liu , Weijie Yang , Jun Cheng","doi":"10.1016/j.biortech.2024.131789","DOIUrl":"10.1016/j.biortech.2024.131789","url":null,"abstract":"<div><div>Nitrogen-doped carbon materials are deemed promising cathode catalysts for microbial fuel cells (MFCs). The challenge lies in reducing costs and enhancing the proportion of electrocatalytically active nitrogenous functional groups. This study proposes a hydrothermal-mediated in-situ doping method to produce nitrogen-doped biochar from aquatic plants. The nitrogen atoms are anchored in the carbon structure during hydrothermal treatment. Subsequent pyrolysis converts the hydrochar into a catalyst with highly catalytically active aromatic ring structure (HC-N+PY). The as-prepared HC-N+PY electrocatalyst demonstrates superior oxygen reduction reaction activity with half-wave potentials of 0.82 V. The MFC with HC-N+PY exhibits excellent performance, with a peak power density of 1444 mW/m<sup>2</sup>. Theoretical calculations demonstrate that the synergistic effect of graphitic nitrogen and C–O groups at defect sites enhances O<sub>2</sub> adsorption and protonation. This work highlights the potential of utilizing nitrogen-doped biochar derived from aquatic plants as an effective catalyst for enhancing the performance of microbial fuel cells.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131789"},"PeriodicalIF":9.7,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-10DOI: 10.1016/j.biortech.2024.131796
Qi He , Qian Zhang , Meng Li , Jing He , Bing Lin , Nan-Ping Wu , Jia-Jing Chen , Xun-Hao Liu , Xiao-Qian Dong
To assess the inherent effects of light–dark cycle on the aniline degradation and nitrogen removal in algal-bacterial symbiotic system, three groups with different photoperiods (0L:12D;6L:6D;12L:0D) were set up. The results revealed that the aniline degradation rate of the three systems all surpassed 99 %, the total nitrogen removal rate of Z2-6L:6D was approximately 36 % higher than Z1-0L:12D eventually, the Z1-0L:12D was restrained by NH4+-N assimilation and nitrification while anoxic denitrification in Z3-12L:0D. The disappearance of microalgae biomass was accompanied by the sharp decreased of polysaccharide in Z1 and longer illumination suppressed the secretion of extracellular polymeric substances, the Z3 yielded slightly superior biomass production despite the double illumination compared with Z2. Moreover, high throughput sequencing analysis illustrated that the microbial community structure in Z2 was more abundant and even than Z3, the TM7a, norank_f__norank_o__Saccharimonadales, Ellin6067 and Scenedesmus proliferated wildly and the photoinhibition to functional genus was effectively alleviated in Z2.
{"title":"Harnessing diurnal dynamics: Understanding the influence of light–dark cycle on algal-bacterial symbiotic system under aniline stress","authors":"Qi He , Qian Zhang , Meng Li , Jing He , Bing Lin , Nan-Ping Wu , Jia-Jing Chen , Xun-Hao Liu , Xiao-Qian Dong","doi":"10.1016/j.biortech.2024.131796","DOIUrl":"10.1016/j.biortech.2024.131796","url":null,"abstract":"<div><div>To assess the inherent effects of light–dark cycle on the aniline degradation and nitrogen removal in algal-bacterial symbiotic system, three groups with different photoperiods (0L:12D;6L:6D;12L:0D) were set up. The results revealed that the aniline degradation rate of the three systems all surpassed 99 %, the total nitrogen removal rate of Z2-6L:6D was approximately 36 % higher than Z1-0L:12D eventually, the Z1-0L:12D was restrained by NH<sub>4</sub><sup>+</sup>-N assimilation and nitrification while anoxic denitrification in Z3-12L:0D. The disappearance of microalgae biomass was accompanied by the sharp decreased of polysaccharide in Z1 and longer illumination suppressed the secretion of extracellular polymeric substances, the Z3 yielded slightly superior biomass production despite the double illumination compared with Z2. Moreover, high throughput sequencing analysis illustrated that the microbial community structure in Z2 was more abundant and even than Z3, the <em>TM7a</em>, <em>norank_f__norank_o__Saccharimonadales</em>, <em>Ellin6067</em> and <em>Scenedesmus</em> proliferated wildly and the photoinhibition to functional genus was effectively alleviated in Z2.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131796"},"PeriodicalIF":9.7,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-10DOI: 10.1016/j.biortech.2024.131784
Yanjun Zhu , Dong Li , Jie Zhang
In an anammox biofilm reactor, long-term operation inevitably leads to the repeated formation of localized dead zones. Once these dead zones (DZs) occur, the anammox reactor’s nitrogen removal efficiency is severely reduced. However, the mechanisms and intrinsic reasons for the transformation of DZs remain unexplored. In this study, the pilot-scale biofilters were classified into biologically active zones (BZs), transition zones (TZs), and DZs. The results indicated that microbial communities undergo accelerated succession from the TZ. Biofilms respond to environmental stress from the DZs by altering the levels of signaling molecules, triggering a series of cascading reactions. These reactions alter the abundance of genes involved in nitrogen removal, promote substance transformation, and speed up the succession of microbial communities. This study demonstrates the objectives and self-healing mechanisms of the anammox biofilm process in the presence of dead zones, which could support the long-term application of anammox technology.
{"title":"Deciphering the dead zone on anammox system in biofilters","authors":"Yanjun Zhu , Dong Li , Jie Zhang","doi":"10.1016/j.biortech.2024.131784","DOIUrl":"10.1016/j.biortech.2024.131784","url":null,"abstract":"<div><div>In an anammox biofilm reactor, long-term operation inevitably leads to the repeated formation of localized dead zones. Once these dead zones (DZs) occur, the anammox reactor’s nitrogen removal efficiency is severely reduced. However, the mechanisms and intrinsic reasons for the transformation of DZs remain unexplored. In this study, the pilot-scale biofilters were classified into biologically active zones (BZs), transition zones (TZs), and DZs. The results indicated that microbial communities undergo accelerated succession from the TZ. Biofilms respond to environmental stress from the DZs by altering the levels of signaling molecules, triggering a series of cascading reactions. These reactions alter the abundance of genes involved in nitrogen removal, promote substance transformation, and speed up the succession of microbial communities. This study demonstrates the objectives and self-healing mechanisms of the anammox biofilm process in the presence of dead zones, which could support the long-term application of anammox technology.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131784"},"PeriodicalIF":9.7,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-09DOI: 10.1016/j.biortech.2024.131792
Zilong Hou , Wenyi Dong , Yanchen Li , Shuo Chen , Huaguang Liu , Qi Han , Zilong Zhao , Jie Liu , Liang Zhang , Hongjie Wang , Yongzhen Peng
The primary challenge of applying partial denitrification/anammox (PD/A) to municipal wastewater treatment lied in the enrichment of functional bacteria with a considerable autotrophic nitrogen removal performance. The results showed influent NO3−-N: NH4+-N, reaction time and temperature would influence anammox nitrogen removal contribution. 15N isotopic tracing technology further revealed the average anammox contribution rate was up to 94.8 %. Extending reaction time was an effective measure to improve simultaneously PD and anammox activity. Microbial community indicated partial denitrifying bacteria (Bacillus) and anammox bacteria (Candidatus Brocadia) were enriched with abundance of 27.27 % and 7.09 % at NO3−-N: NH4+-N of 1:1. The correlation analysis showed that NO3−-N: NH4+-N ratio played the positive role for Bacillus enrichment, and low temperature was favorable to the enrichment of Thauera and Candidatus Jettenia. Overall, this study demonstrated the reasonable operational strategy would strengthen anammox contribution and facilitate enrichment of functional bacteria.
{"title":"Achieving efficient anammox contribution and the enrichment of functional bacteria in partial denitrification/anammox system: Performance, microbial evolution and correlation analysis","authors":"Zilong Hou , Wenyi Dong , Yanchen Li , Shuo Chen , Huaguang Liu , Qi Han , Zilong Zhao , Jie Liu , Liang Zhang , Hongjie Wang , Yongzhen Peng","doi":"10.1016/j.biortech.2024.131792","DOIUrl":"10.1016/j.biortech.2024.131792","url":null,"abstract":"<div><div>The primary challenge of applying partial denitrification/anammox (PD/A) to municipal wastewater treatment lied in the enrichment of functional bacteria with a considerable autotrophic nitrogen removal performance. The results showed influent NO<sub>3</sub><sup>−</sup>-N: NH<sub>4</sub><sup>+</sup>-N, reaction time and temperature would influence anammox nitrogen removal contribution. <sup>15</sup>N isotopic tracing technology further revealed the average anammox contribution rate was up to 94.8 %. Extending reaction time was an effective measure to improve simultaneously PD and anammox activity. Microbial community indicated partial denitrifying bacteria (<em>Bacillus</em>) and anammox bacteria (<em>Candidatus Brocadia</em>) were enriched with abundance of 27.27 % and 7.09 % at NO<sub>3</sub><sup>−</sup>-N: NH<sub>4</sub><sup>+</sup>-N of 1:1. The correlation analysis showed that NO<sub>3</sub><sup>−</sup>-N: NH<sub>4</sub><sup>+</sup>-N ratio played the positive role for <em>Bacillus</em> enrichment, and low temperature was favorable to the enrichment of <em>Thauera</em> and <em>Candidatus Jettenia</em>. Overall, this study demonstrated the reasonable operational strategy would strengthen anammox contribution and facilitate enrichment of functional bacteria.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131792"},"PeriodicalIF":9.7,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-09DOI: 10.1016/j.biortech.2024.131793
Ding Ma, Lisha Shi, Qiangqiang Xia, Tao Xu
Pseudomonas and Bacillus are dominant microorganisms to widely distributes in asphalt pavement structure. Microbial degradation leads to asphalt aging, and causes its performance deterioration, reducing the durability of asphalt pavement. To better understand the degradation behaviors of dominant microorganisms on asphalt, and reveals their microbial aging mechanisms on asphalt, the effects of microbial degradation on micromorphology, chemical functional group, component and microstructure of asphalt were discussed. Results indicate that main damages of microbial degradation start from asphalt surface and then permeates into its interior. Microorganisms degrade light components of asphalt as nutrients and decompose them into CO2 and H2O through oxidation reactions, but exhibit limited degradation ability to macromolecular components. Microbial degradation causes the content changes of asphalt components, altering the colloidal structure of asphalt. Microorganisms destroy the continuity of asphalt surface and increase surface roughness of asphalt. This study provides a new insight into microbial aging mechanism of asphalt.
假单胞菌和芽孢杆菌是广泛分布于沥青路面结构中的主要微生物。微生物降解会导致沥青老化，使其性能下降，降低沥青路面的耐久性。为了更好地了解优势微生物对沥青的降解行为，揭示其对沥青的微生物老化机理，探讨了微生物降解对沥青微观形态、化学官能团、组分和微观结构的影响。结果表明，微生物降解的主要破坏是从沥青表面开始，然后向内部渗透。微生物将沥青中的轻质成分作为营养物质降解，并通过氧化反应将其分解为 CO2 和 H2O，但对大分子成分的降解能力有限。微生物降解会引起沥青成分的含量变化，改变沥青的胶体结构。微生物会破坏沥青表面的连续性，增加沥青表面的粗糙度。这项研究为沥青的微生物老化机理提供了新的见解。
{"title":"Degradation behaviors of asphalt by microorganisms in asphalt pavement structure","authors":"Ding Ma, Lisha Shi, Qiangqiang Xia, Tao Xu","doi":"10.1016/j.biortech.2024.131793","DOIUrl":"10.1016/j.biortech.2024.131793","url":null,"abstract":"<div><div><em>Pseudomonas</em> and <em>Bacillus</em> are dominant microorganisms to widely distributes in asphalt pavement structure. Microbial degradation leads to asphalt aging, and causes its performance deterioration, reducing the durability of asphalt pavement. To better understand the degradation behaviors of dominant microorganisms on asphalt, and reveals their microbial aging mechanisms on asphalt, the effects of microbial degradation on micromorphology, chemical functional group, component and microstructure of asphalt were discussed. Results indicate that main damages of microbial degradation start from asphalt surface and then permeates into its interior. Microorganisms degrade light components of asphalt as nutrients and decompose them into CO<sub>2</sub> and H<sub>2</sub>O through oxidation reactions, but exhibit limited degradation ability to macromolecular components. Microbial degradation causes the content changes of asphalt components, altering the colloidal structure of asphalt. Microorganisms destroy the continuity of asphalt surface and increase surface roughness of asphalt. This study provides a new insight into microbial aging mechanism of asphalt.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131793"},"PeriodicalIF":9.7,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The surge in population growth, urbanization, and shifts in food consumption patterns have resulted in a rise in the global production of organic waste. This waste material must be repurposed and effectively managed to minimize environmental footprints. The generation of abundant biowaste, especially from marine sources, may have detrimental impacts on the environment and human health if left untreated. In recent years, substantial efforts have been made to valorize seafood waste, contributing significantly to the sustainability of the blue economy through the repurposing of marine discards. Seafood waste can be transformed into different by-products which can be applied as soil amendment to enhance soil quality and health, demonstrating a holistic approach to repurposing and waste utilization. The extraction of bioactive metabolites from these waste materials has opened avenues for developing nanofertilizers. This intersection of waste valorization and nanotechnology is pertinent in the context of sustainable agriculture. While conventional fertilizers improve soil fertility with significant leaching and gaseous losses, the advent of nanofertilizers introduces a paradigm shift with their targeted and controlled delivery mechanisms, rendering them significantly more efficient in resource utilization and mitigation of environmental crises. This review delves into the global issue of seafood waste accumulation, offering an overview of various methods for repurposing. The primary aim of this review is to bring into limelight the recent efforts in developing a portfolio of carbon-based nanofertilizers derived from organic waste, replacing previous valorization methods due to their sustainability, efficiency, and eco-friendliness. There are immense opportunities for future work in this direction by exploring innovative nanoengineering approach owing to the potential of carbon nanofertilizers in enhancing the production of value-added products and reduction of environmental pollution.
{"title":"Valorization and repurposing of seafood waste to next-generation carbon nanofertilizers","authors":"Rabia Javed , Uzma Sharafat , Ayesha Rathnayake , Lakshman Galagedara , Gurpreet Singh Selopal , Raymond Thomas , Mumtaz Cheema","doi":"10.1016/j.biortech.2024.131783","DOIUrl":"10.1016/j.biortech.2024.131783","url":null,"abstract":"<div><div>The surge in population growth, urbanization, and shifts in food consumption patterns have resulted in a rise in the global production of organic waste. This waste material must be repurposed and effectively managed to minimize environmental footprints. The generation of abundant biowaste, especially from marine sources, may have detrimental impacts on the environment and human health if left untreated. In recent years, substantial efforts have been made to valorize seafood waste, contributing significantly to the sustainability of the blue economy through the repurposing of marine discards. Seafood waste can be transformed into different by-products which can be applied as soil amendment to enhance soil quality and health, demonstrating a holistic approach to repurposing and waste utilization. The extraction of bioactive metabolites from these waste materials has opened avenues for developing nanofertilizers. This intersection of waste valorization and nanotechnology is pertinent in the context of sustainable agriculture. While conventional fertilizers improve soil fertility with significant leaching and gaseous losses, the advent of nanofertilizers introduces a paradigm shift with their targeted and controlled delivery mechanisms, rendering them significantly more efficient in resource utilization and mitigation of environmental crises. This review delves into the global issue of seafood waste accumulation, offering an overview of various methods for repurposing. The primary aim of this review is to bring into limelight the recent efforts in developing a portfolio of carbon-based nanofertilizers derived from organic waste, replacing previous valorization methods due to their sustainability, efficiency, and eco-friendliness. There are immense opportunities for future work in this direction by exploring innovative nanoengineering approach owing to the potential of carbon nanofertilizers in enhancing the production of value-added products and reduction of environmental pollution.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131783"},"PeriodicalIF":9.7,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-09DOI: 10.1016/j.biortech.2024.131771
Xueyao Zhang , Zhaohui An , Jiefu Wang , Stephanie Lansing , Naresh Kumar Amradi , Md. Sazzadul Haque , Zhi-Wu Wang
Food waste digestate was fed into a sequencing batch reactor (SBR) for Haloferax mediterranei (HM) to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). This SBR was operated uninterruptedly for 450 days to test its stability, during which the cycle time and volume exchange ratio were varied to understand their impacts on the PHBV fermentation performance under ranged organic loading rates (OLR). Results showed that 1) PHBV productivity was proportional to OLR of food waste digestate; 2) substrate and product inhibitions were two limiting factors constraining substrate utilization and PHBV yields; 3) PHBV titer was dependent on the hydraulic retention time of the SBR while a volume exchange ratio lower than 0.5 is unfavorable due to the product inhibitor accumulation. This study for the first time demonstrated that the long-term stability of food waste-fed PHBV production by HM and revealed that inhibition effects could be barriers in SBR limiting the full-scale application of the technology.
{"title":"Long-term effects of cycle time and volume exchange ratio on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production from food waste digestate by Haloferax mediterranei cultivated in sequencing batch reactors for 450 days","authors":"Xueyao Zhang , Zhaohui An , Jiefu Wang , Stephanie Lansing , Naresh Kumar Amradi , Md. Sazzadul Haque , Zhi-Wu Wang","doi":"10.1016/j.biortech.2024.131771","DOIUrl":"10.1016/j.biortech.2024.131771","url":null,"abstract":"<div><div>Food waste<!--> <!-->digestate<!--> <!-->was fed into a sequencing batch reactor (SBR) for<!--> <em>Haloferax mediterranei</em> <!-->(<em>HM</em>) to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). This SBR was operated uninterruptedly for 450 days to test its stability, during which the cycle time and volume exchange ratio<!--> <!-->were varied to understand their impacts on the PHBV fermentation performance under ranged organic loading rates (OLR). Results showed that 1) PHBV productivity was proportional to OLR of food waste<!--> <!-->digestate; 2) substrate and product inhibitions were two limiting factors constraining substrate utilization and PHBV yields; 3) PHBV titer was dependent on the hydraulic retention time of the SBR while a volume exchange ratio lower than 0.5 is unfavorable due to the product inhibitor accumulation. This study for the first time demonstrated that the long-term stability of food waste-fed PHBV production by<!--> <em>HM</em> <!-->and revealed that inhibition effects could be barriers in SBR limiting the full-scale application of the technology.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131771"},"PeriodicalIF":9.7,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-09DOI: 10.1016/j.biortech.2024.131791
Nan Zhang , Dong Zhu , Zhiyuan Yao , David Z. Zhu
As a vital part of microbial communities, viruses in constructed wetlands (CWs) remain poorly explored, yet they could significantly affect pollutant removal. Here, two pilot-scale CWs were built to investigate the viral community under different hydraulic loading rates (HLRs) using in-depth metagenomic analysis. Gene-sharing networks suggested that the CWs were pools of unexplored viruses. A higher abundance of prokaryotic functional genes related to sulfur cycling and denitrification was observed in the higher HLR condition, which was associated with greater removal of total nitrogen and nitrate nitrogen compared to the lower HLR condition. Viruses also affect nitrogen pollutant removal by potentially infecting functional prokaryotes, such as denitrification bacteria and ammonia-oxidizing bacteria, and by providing auxiliary metabolic genes involved in sulfur and nitrogen cycling. These findings reveal the significance of viruses in pollutant removal in CWs and enhance the understanding of the relationship between engineering design parameters and performance from microbial perspectives.
{"title":"Virus-prokaryote interactions assist pollutant removal in constructed wetlands","authors":"Nan Zhang , Dong Zhu , Zhiyuan Yao , David Z. Zhu","doi":"10.1016/j.biortech.2024.131791","DOIUrl":"10.1016/j.biortech.2024.131791","url":null,"abstract":"<div><div>As a vital part of microbial communities, viruses in constructed wetlands (CWs) remain poorly explored, yet they could significantly affect pollutant removal. Here, two pilot-scale CWs were built to investigate the viral community under different hydraulic loading rates (HLRs) using in-depth metagenomic analysis. Gene-sharing networks suggested that the CWs were pools of unexplored viruses. A higher abundance of prokaryotic functional genes related to sulfur cycling and denitrification was observed in the higher HLR condition, which was associated with greater removal of total nitrogen and nitrate nitrogen compared to the lower HLR condition. Viruses also affect nitrogen pollutant removal by potentially infecting functional prokaryotes, such as denitrification bacteria and ammonia-oxidizing bacteria, and by providing auxiliary metabolic genes involved in sulfur and nitrogen cycling. These findings reveal the significance of viruses in pollutant removal in CWs and enhance the understanding of the relationship between engineering design parameters and performance from microbial perspectives.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131791"},"PeriodicalIF":9.7,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pioneer facilities for Sustainable Aviation Fuels (SAF) convert fats, oils, and grease into hydrocarbons using the Hydroprocessed Esters and Fatty Acids (HEFA) technology. However, limited feedstock availability and sustainability concerns may restrict broader adoption. Biotechnology offers an alternative by enabling microbial oil production from sugars, expanding the feedstock portfolio with more productive biomass sources or waste materials. This study assessed the economic and environmental impacts of SAF production through HEFA using microbial oil from sugarcane, combining achievable fermentation performance with mature catalytic conversion. The results demonstrated SAF costs between $1.83 and $3.00 per liter and over 50 % reduction in greenhouse gas emissions compared to fossil fuels. Sensitivity analysis identified fermentation performance as the key factor driving these outcomes. Additionally, this approach yielded higher SAF per hectare than soybean-oil-based HEFA, potentially reducing emissions from land-use change.
可持续航空燃料（SAF）的先驱设施利用加氢处理酯和脂肪酸（HEFA）技术将脂肪、油和油脂转化为碳氢化合物。然而，有限的原料供应和可持续性问题可能会限制更广泛的应用。生物技术提供了一种替代方法，即利用微生物从糖类中生产油脂，并利用更高产的生物质来源或废料扩大原料组合。本研究评估了利用甘蔗中的微生物油通过 HEFA 生产 SAF 的经济和环境影响，将可实现的发酵性能与成熟的催化转化相结合。结果表明，与化石燃料相比，SAF 的成本在每升 1.83 美元到 3.00 美元之间，温室气体排放量减少了 50%以上。敏感性分析表明，发酵性能是产生这些结果的关键因素。此外，与基于大豆油的 HEFA 相比，这种方法的每公顷 SAF 产量更高，有可能减少因土地使用变化而产生的排放。
{"title":"Alternative feedstocks for sustainable aviation fuels: Assessment of sugarcane-derived microbial oil","authors":"Andressa Neves Marchesan, Isabelle Lobo de Mesquita Sampaio, Mateus Ferreira Chagas, Wesley Cardoso Generoso, Thayse Aparecida Dourado Hernandes, Edvaldo Rodrigo Morais, Tassia Lopes Junqueira","doi":"10.1016/j.biortech.2024.131772","DOIUrl":"10.1016/j.biortech.2024.131772","url":null,"abstract":"<div><div>Pioneer facilities for Sustainable Aviation Fuels (SAF) convert fats, oils, and grease into hydrocarbons using the Hydroprocessed Esters and Fatty Acids (HEFA) technology. However, limited feedstock availability and sustainability concerns may restrict broader adoption. Biotechnology offers an alternative by enabling microbial oil production from sugars, expanding the feedstock portfolio with more productive biomass sources or waste materials. This study assessed the economic and environmental impacts of SAF production through HEFA using microbial oil from sugarcane, combining achievable fermentation performance with mature catalytic conversion. The results demonstrated SAF costs between $1.83 and $3.00 per liter and over 50 % reduction in greenhouse gas emissions compared to fossil fuels. Sensitivity analysis identified fermentation performance as the key factor driving these outcomes. Additionally, this approach yielded higher SAF per hectare than soybean-oil-based HEFA, potentially reducing emissions from land-use change.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131772"},"PeriodicalIF":9.7,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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